CN104067936A - Method for promoting nervilia fordii corms to grow more leaf buds - Google Patents
Method for promoting nervilia fordii corms to grow more leaf buds Download PDFInfo
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- CN104067936A CN104067936A CN201410272750.4A CN201410272750A CN104067936A CN 104067936 A CN104067936 A CN 104067936A CN 201410272750 A CN201410272750 A CN 201410272750A CN 104067936 A CN104067936 A CN 104067936A
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- ford nervilia
- nervilia leaf
- bud
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 241000174387 Nervilia fordii Species 0.000 title abstract description 7
- 230000001737 promoting effect Effects 0.000 title abstract description 4
- 229940088597 hormone Drugs 0.000 claims abstract description 10
- 239000005556 hormone Substances 0.000 claims abstract description 10
- 241001314539 Nervilia Species 0.000 claims description 79
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 31
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 31
- 229960001669 kinetin Drugs 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 150000001875 compounds Chemical class 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 4
- 238000003475 lamination Methods 0.000 claims description 4
- 230000003020 moisturizing effect Effects 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 230000000284 resting effect Effects 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 210000002615 epidermis Anatomy 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 206010013786 Dry skin Diseases 0.000 claims description 2
- 244000037666 field crops Species 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 3
- 241000510678 Falcaria vulgaris Species 0.000 description 8
- 206010011224 Cough Diseases 0.000 description 2
- 241000233855 Orchidaceae Species 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 201000007227 lymph node tuberculosis Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for promoting nervilia fordii corms to grow more leaf buds. The method comprises the following steps: 1, carrying out earlier stage processing on the nervilia fordii corms; 2, preparing a hormone solution with a certain concentration; 3, immersing the corms; 4, cultivating the corms; 5, cutting off buds from the corms which only have one bud; 6, immersing the corms without the buds into the hormone solution; 7, cultivating the corms without the buds; and 8, transplanting. The method has the advantages that the method provided by the invention enables more than 84.8% of the nervilia fordii corms to annually grow two or more than two leaves from a previous condition of annually growing one leaf, so that the annual output and the yield of the unit area of nervilia fordii are improved; a technical support is provided for increasing the output of nervilia fordii medicinal materials and promoting the sustainable development.
Description
Technical field
The invention belongs to agronomy, biology, pharmaceutical field.Relate to a kind of how long method of leaf bud of plant bulb of impelling, specifically a kind of how long method of leaf bud of ford nervilia leaf bulb of impelling.
Background technology
Ford nervilia leaf is the dried leaves of the orchid family (Orchidaceae) plant Nervilia (Nervilia) Nervilia fordii Nervilia fordii (Heance) Schltr. or the leaf with bulb, belongs to perennial root herbelet.Be distributed in the ground such as Guangxi, Guangdong, Sichuan, Yunnan.Cool in nature, taste is sweet, have moisten the lung and relieve the cough, invigorating the spleen disappears long-pending, clearing heat and detoxicating, blood stasis removing analgesic, cures mainly the diseases such as tuberculosis, cough hemoptysis, scrofula, bronchitis.Because the market demand is large and ford nervilia leaf self leans on propagation by corm, reproduction rate is extremely low, and the destruction of ecotope, is rare medicinal material in imminent danger at present.Therefore, improving the culture technique of ford nervilia leaf, increase year output and the unit are output of ford nervilia leaf, is a kind of effective way that solves ford nervilia leaf MED SUP deficiency and sustainable use.But the culture technique of ford nervilia leaf is with propagation by corm at present, bulb is only grown once for 1 year, and only long a slice leaf, the general Only Six Months of growth time (March is then to August), bulb is in resting state At All Other Times, and for how impelling ford nervilia leaf bulb, within breeding time, how long leaf bud there is no bibliographical information at present.This patent impels how long leaf bud of ford nervilia leaf bulb, and leaf bud develops into blade, and bulb of ford nervilia leaf can grow two and two with blade, for further investigation, improve ford nervilia leaf output and Sustainable Development and Utilization provides reference.
Summary of the invention
The present invention provides a kind of how long method of leaf bud of ford nervilia leaf bulb of impelling for making up the deficiencies in the prior art.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
Impel a how long method for leaf bud of ford nervilia leaf bulb, it is characterized in that, method comprises the following steps:
1. process the early stage of ford nervilia leaf bulb
From the matrix of field or cultivation ford nervilia leaf, dig out in the full ford nervilia leaf bulb without mildew or stain of resting state bulb, rinse out the silt above bulb with running water, dry, bulb epidermis is slightly dry anhydrous.
2. compound concentration is 10ppm～80ppm hormone solution
Compound method, get the kinetin powder of-20 DEG C, accurately take 0.01g and pour in test tube, then adding 2ml volumetric concentration is that 95% ethanol dissolves, after kinetin dissolves completely, the kinetin solution that is 10ppm～80ppm with distilled water or pure water compound concentration.
3. bulb soaks
The ford nervilia leaf bulb that step 1 is processed is put into and the hormone solution that step 2 prepares is housed is soaked, and hormone solution need soak all ford nervilia leaf bulbs completely; Solution concentration is respectively 5ppm, 10ppm, 20ppm, 40ppm and 80ppm; Soak time is 10 minutes, 30 minutes, 1 hour, 6 hours and 12 hours.
The ford nervilia leaf bulb that step 1 is handled well is put into and is filled the kinetin solution that step 2 prepares and soak, and kinetin solution need soak all ford nervilia leaf bulbs completely, and hormone concentration is 10ppm～80ppm, and soak time is 10 minutes～12 hours.
4. bulb is cultivated
Soaked step 3 ford nervilia leaf bulb is put into clear glass culture dish to be placed with on 2～3 layers of filter paper being soaked into by pure water or distilled water, bulb can not be piled up lamination, vessel cover upper cover, moisturizing, and be put in incubator and cultivate, incubator temperature is 18 DEG C, 22 DEG C, 25 DEG C, 28 DEG C, 31 DEG C, wherein temperature 25 DEG C～31 DEG C better, best with 28 DEG C.In the process of cultivating, often observe, keep filter paper moistening, keep humidity 80～95%.The general ford nervilia leaf bulb of cultivating through this stage how long the probability of leaf bud more than 24%.
Above-mentioned incubation time: bulb is put in incubator and cultivates, time length determines depending on cultivation temperature and germinating growth situation, in 18 DEG C and 22 DEG C of incubators, cultivates, bulb germinating time is longer, within ten days, just has above leaf bud to grow from bulb, and poor growth.Relatively in the incubator of 25 DEG C, 28 DEG C and 31 DEG C, cultivate, bulb germinating time was at 8～10 days, and 100% germinates, and bud grows fine.
5. cut bud
The ford nervilia leaf bulb of cultivating through incubator, more than 24% bulb grows polygerm, to only growing the ford nervilia leaf bulb of a leaf bud, needs with the blade excision leaf bud through sterilization, and excision is that bud length exceedes i.e. excision after 0.3cm opportunity.
6. cut gemmule stem soaks in hormone solution
By step 5) the ford nervilia leaf bulb processed puts that indoor to dry bulb skin dry anhydrous, and being then immersed in concentration is in 10ppm～80ppm kinetin solution, and soak time is 10 minutes～12 hours.
7. cutting gemmule stem cultivates
By step 6) the gemmule stem of cutting after soaking puts in incubator and cultivates, and cultural method is with step 4.
Cut gemmule stem and in incubator, cultivate 18 days～20 days, now cut germinate 2 buds and more than 2 buds of gemmule stem;
8. polygerm bulb and the transplanting of cutting bud polygerm bulb
By step 4) the gemmule stem of cutting cultivated of the polygerm bulb cultivated and step 7. germinates after the sterilization of 2 buds and ford nervilia leaf bulbs more than 2 buds and transplants land for growing field crops, and ford nervilia leaf bulb sum polygerm probability reaches more than 84.8%.
Advantage of the present invention:
Raw material of the present invention be easy to get and nontoxic, method of operating is easy, cost is low;
2. long more than 2 and 2 the leaf bud probability of ford nervilia leaf bulb of the present invention is high, and how long leaf bud probability is up to more than 84.8% for bulb;
3. tool of the present invention is stronger practicality and promotional value.Application of the present invention, can make ford nervilia leaf bulb a slice leaf of only growing for a year change growth two and two into blade, and improve the yield per unit area of ford nervilia leaf and improve annual earnings, be to solve current ford nervilia leaf medicinal material a kind of method in short supply.
Embodiment
Below in conjunction with the embodiment of the present invention, the invention will be further described.
Embodiment 1
Impel the how long method of leaf bud of ford nervilia leaf bulb
1. process the early stage of ford nervilia leaf bulb
From the matrix of field or cultivation ford nervilia leaf, dig out in the full ford nervilia leaf bulb without mildew or stain of resting state bulb, rinse out the silt above bulb with running water, dry, bulb epidermis is slightly dry anhydrous;
2. compound concentration is 10ppm kinetin solution
Compound method, gets the kinetin powder of-20 DEG C of preservations, accurately takes 0.01g and pours in test tube, and then adding 2ml volumetric concentration is that 95% ethanol dissolves, after kinetin dissolves completely, and the kinetin solution that is 10ppm with distilled water or pure water compound concentration;
3. bulb soaks
The ford nervilia leaf bulb that step 1 is handled well is put into and is filled the kinetin solution that step 2 prepares and soak, and kinetin solution need soak all ford nervilia leaf bulbs completely, and soak time is respectively 10 minutes, and 30 minutes, 1 hour, 6 hours, 12 hours;
4. bulb is cultivated
Soaked step 3 ford nervilia leaf bulb is put into clear glass culture dish, in ware, be placed with 2～3 layers of filter paper being soaked into by pure water or distilled water, ford nervilia leaf bulb after immersion is placed on filter paper, and bulb can not be piled up lamination, vessel cover upper cover, moisturizing, then being put into temperature is to cultivate 20 days in 25 DEG C of incubators, in the process of cultivating, often observes, keep filter paper moistening, humidity is controlled at 80～95%;
5. observe soak time to the how long impact of leaf bud of ford nervilia leaf bulb
From the result of the test of table 1, bulb soaks the ford nervilia leaf bulb of 10 minutes how long leaf bud probability is minimum is 40%, and bulb soaks how long leaf bud maximum probability was for reaching 70% in 6 hours and 12 hours.
Table 1 soak time is on the how long impact of leaf bud of ford nervilia leaf bulb
Embodiment 2
Impel the how long method of leaf bud of ford nervilia leaf bulb:
1. process the early stage of ford nervilia leaf bulb
With embodiment 1 step 1
2. preparation variable concentrations kinetin solution
Compound method, get the kinetin powder of-20 DEG C of preservations, accurately taking 0.01g pours in test tube, then adding 2ml volumetric concentration is that 95% ethanol dissolves, after kinetin dissolves completely, the kinetin solution that is 10ppm with distilled water or pure water compound concentration, 20ppm, the kinetin solution concentration of 40ppm and 80ppm is prepared with reference to the method;
3. bulb soaks
The ford nervilia leaf bulb that step 1 is handled well is put into and is filled the kinetin solution that step 2 prepares and soak, and soaks 30 minutes, and kinetin solution need soak all ford nervilia leaf bulbs completely;
4. bulb is cultivated
Soaked step 3 ford nervilia leaf bulb is put into respectively to clear glass culture dish, in ware, be placed with 2～3 layers of filter paper being soaked into by pure water or distilled water, ford nervilia leaf bulb after immersion is placed on filter paper, and bulb can not be piled up lamination, vessel cover upper cover, moisturizing, then being put into temperature is to cultivate 20 days in 28 DEG C of incubators, in the process of cultivating, often observes, keep filter paper moistening, humidity is controlled at 80～95%;
5. observe different kinetin concentration to the how long impact of leaf bud of ford nervilia leaf bulb
From the result of the test of table 2, through the cultivation of 20 days, how long the germination rate of leaf bud was minimum 60% to soak 10ppm ford nervilia leaf bulb, soak 20ppm and 80ppm bulb how long the germination rate of leaf bud be up to 80%.Therefore under the condition of culture of 28 DEG C, two factors of soak time and kinetin solution concentration to ford nervilia leaf bulb how long the minimum probability of leaf bud be 24% (40% × 60%).
Table 2 hormon concentration is on the how long impact of leaf bud of ford nervilia leaf bulb
Embodiment 3
Impel the how long method of leaf bud of ford nervilia leaf bulb:
1. process the early stage of ford nervilia leaf bulb
With embodiment 1 step 1
2. compound concentration is 10ppm kinetin solution
With embodiment 1 step 2
3. bulb soaks
With embodiment 2 steps 3
4. bulb is cultivated
With embodiment 1 step 4, just incubator temperature is respectively 18 DEG C, and 21 DEG C, 25 DEG C, 28 DEG C and 31 DEG C
5. observe cultivation temperature to the how long impact of leaf bud of ford nervilia leaf bulb
From the result of the test of table 3, how long leaf bud is under the cultivation temperature of 18 DEG C and 21 DEG C for ford nervilia leaf bulb, and growth rate is slower, cultivate how long leaf bud probability was 20% in 20 days for 21 DEG C, and 25 DEG C, 28 DEG C and 31 DEG C cultivate 20 days how long the probability of leaf bud be respectively 60%, 63% and 80%.Therefore, how long the cultivation temperature of leaf bud generally should be more than 25 DEG C for ford nervilia leaf bulb.
Table 3 different temperatures is on the how long impact of leaf bud of ford nervilia leaf bulb
Embodiment 4
Impel the how long method of leaf bud of ford nervilia leaf bulb:
1. process the early stage of ford nervilia leaf bulb
With embodiment 1 step 1
2. preparation variable concentrations kinetin solution
With embodiment 2 steps 2
3. bulb soaks
With embodiment 2 steps 3
4. bulb is cultivated
With embodiment 2 steps 4
5. observe different kinetin concentration ford nervilia leaf is cut to the how long impact of leaf bud of gemmule stem
Table 4 variable concentrations kinetin is on cutting the how long impact of leaf bud of gemmule stem
From the result of the test of table 4, to cut gemmule stem and cultivate the 8th day, the ford nervilia leaf bulb how long minimum probability of leaf bud reaches 80%, and how long leaf bud minimum probability is 60.8% (76% × 80%=60.8%) to cut gemmule stem.
By above embodiment, the probability that ford nervilia leaf bulb utilizes its polygerm bulb of this patent method and cuts bud polygerm bulb sum is more than 84.8% (24%+60.8%).
Claims (1)
1. impel a how long method for leaf bud of ford nervilia leaf bulb, it is characterized in that, method comprises the following steps:
1) process the early stage of ford nervilia leaf bulb
From the matrix of field or cultivation ford nervilia leaf, dig out in the full ford nervilia leaf bulb without mildew or stain of resting state bulb, rinse out the silt above bulb with running water, dry, bulb epidermis is slightly dry anhydrous;
2) compound concentration is 10ppm～80ppm hormone solution
Compound method, get the kinetin powder of-20 DEG C, accurately take 0.01g and pour in test tube, then adding 2ml volumetric concentration is that 95% ethanol dissolves, after kinetin dissolves completely, the kinetin solution that is 10ppm～80ppm with distilled water or pure water compound concentration;
3) bulb soaks
By step 1) the ford nervilia leaf bulb handled well puts into and fills step 2) the kinetin solution of preparation soaks, kinetin solution need soak all ford nervilia leaf bulbs completely, and hormone concentration is 10ppm～80ppm, and soak time is 10 minutes～12 hours;
4) bulb is cultivated
By step 3) soaked ford nervilia leaf bulb puts into clear glass culture dish, in ware, be placed with 2～3 layers and soaked pure water or the drenched filter paper of distilled water, the ford nervilia leaf bulb after immersion is placed on filter paper, and bulb can not be piled up lamination, vessel cover upper cover, moisturizing, is then put in incubator and cultivates 6～20 days, and incubator temperature is 25 DEG C～31 DEG C, in the process of cultivating, often observe, keep filter paper moistening, humidity remains on 80～95%;
Described incubation time: bulb is put in incubator and cultivates, time length is determined depending on cultivation temperature and germinating growth situation, in the incubator of 25 DEG C～31 DEG C, cultivates, bulb germinating time was at 8～10 days;
5) cut bud
The ford nervilia leaf bulb of cultivating through incubator, more than 24% bulb grows polygerm, to only growing the ford nervilia leaf bulb of a leaf bud, needs with the blade excision leaf bud through sterilization, and excision opportunity is that bud length exceedes 0.3cm;
6) cut gemmule stem soaks in hormone solution
By step 5) the ford nervilia leaf bulb processed puts that indoor to dry bulb skin dry anhydrous, and being then immersed in concentration is in 10ppm～80ppm kinetin solution, and soak time is 10 minutes～12 hours;
7) cutting gemmule stem cultivates
By step 6) the gemmule stem of cutting after soaking puts in incubator and cultivates, and cultural method is with step 4;
Cut gemmule stem and in incubator, cultivate 18 days～20 days, now cut germinate 2 buds and more than 2 buds of gemmule stem;
8) polygerm bulb and the transplanting of cutting bud polygerm bulb
By step 4) polygerm bulb and the step 7 of cultivating) the gemmule stem of cutting cultivated germinates after the sterilization of 2 buds and ford nervilia leaf bulbs more than 2 buds and transplants land for growing field crops, and ford nervilia leaf bulb sum polygerm probability reaches more than 84.8%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105052749A (en) * | 2015-09-08 | 2015-11-18 | 莫玉明 | Tissue culture seedling raising method of nervilia fordii |
| CN105165344A (en) * | 2015-09-11 | 2015-12-23 | 莫玉明 | Cutting seedling method of nervilia fordii |
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| CN102134561A (en) * | 2010-12-30 | 2011-07-27 | 广东南台药业有限公司 | Culture medium used for fast breeding tissue-culture root-shaped stems of nervilia fordii |
| CN103535275A (en) * | 2012-07-15 | 2014-01-29 | 云南省德宏热带农业科学研究所 | Tissue-culture rapid multiplication method of nerviliae fordii corm |
| CN103766039A (en) * | 2014-02-18 | 2014-05-07 | 广西壮族自治区药用植物园 | Method for breaking dormancy of nervilis fordii schlecht bulb |
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2014
- 2014-06-18 CN CN201410272750.4A patent/CN104067936B/en active Active
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| CN102134561A (en) * | 2010-12-30 | 2011-07-27 | 广东南台药业有限公司 | Culture medium used for fast breeding tissue-culture root-shaped stems of nervilia fordii |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052749A (en) * | 2015-09-08 | 2015-11-18 | 莫玉明 | Tissue culture seedling raising method of nervilia fordii |
| CN105165344A (en) * | 2015-09-11 | 2015-12-23 | 莫玉明 | Cutting seedling method of nervilia fordii |
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Application publication date: 20141001 Assignee: Cenxi Xulong Agricultural Technology Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045868 Denomination of invention: A Method of Promoting Long Leaf Buds in Green Sky Sunflower Bulbs Granted publication date: 20160427 License type: Common License Record date: 20231106 |
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