CN105850729A - Verbena bonariensis cultivation method - Google Patents
Verbena bonariensis cultivation method Download PDFInfo
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- CN105850729A CN105850729A CN201610208072.4A CN201610208072A CN105850729A CN 105850729 A CN105850729 A CN 105850729A CN 201610208072 A CN201610208072 A CN 201610208072A CN 105850729 A CN105850729 A CN 105850729A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a verbena bonariensis cultivation method. The verbena bonariensis cultivation method comprises a pre-treatment step, a low-temperature sleeping treatment stage, a seed germination stage, a strong seedling transplanting stage, and a nutritional solution culture stage. The method is simple and is easy to operate; seedling development time is relatively short and the specification consistency is high; and the dosage of compounds is greatly reduced, the pollution generated by a nutritional solution is reduced, and the tissue culture effect is good.
Description
Technical field
The present invention relates to field of plant growing technology, be specifically related to a kind of Folium Salicis Babylonicae Herba Verbenae breeding method.
Background technology
Herba Verbenae majority is grown on open country, and in China East China, also there is distribution on the ground such as south China, its have removing heat from blood, dissipating blood stasis, heat clearing away, removing toxic substances,
The effect such as antipruritic, therefore becomes a kind of important draft, and the yield of Herba Verbenae little at present, and cultivation period is long before existing, and
Quality is the highest, and excellent seed is the primary condition that can obtain high yield, and the nutrient solution culture of optimization is bred as commonly using for Herba Verbenae cultivating seeds
Mode, the method limitation in prior art, Folium Salicis Babylonicae Herba Verbenae cultivated, do not have a kind of economic and environment-friendly, the culture fluid of of low pollution, and raw
Long period is long.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of economic and environment-friendly, and pollute little, the Folium Salicis Babylonicae that group training is effective
Herba Verbenae breeding method.
The invention provides a kind of Folium Salicis Babylonicae Herba Verbenae breeding method, it is characterised in that: comprise the steps:
(1) pre-treatment step: disease-free Folium Salicis Babylonicae Herba Verbenae seed is soaked 48h with deionized water under conditions of room temperature and cleans up
After, under sunlight condition, dry in the sun 1-3d, then carries out disinfection to seed；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, is being placed in
5-10d in the environment of 10 DEG C；
(3) in the germination stage: step (2) gained seed is placed in deionized water immersion, soak time is 6-12 hour, then
Taking out and cover its surface with wet cloth, treat germination, arrange constant germination temperature, germination temperature is 30～33 DEG C, aseptic
Under conditions of every day in the set time, seed is sprayed water, under sterile conditions, bud point is cut, by bud point move into have basis training
Support and the container of base carries out basis cultivation；
(4) transplant the stage in strong sprout: seed detoxic seedling is carried out expanding propagation cultivation, seed detoxic seedling is cultivated in expanding propagation culture medium,
After germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is quartz sand；
(5) in the Solution culture method stage: when growth of seedling to two panels true leaf, move on to nutritional solution normal growth from quartz sand or carry out big
Field is planted；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Preferably, described in step (1), sterilization method is: the seed going out decontamination is soaked in 40% sulfuric acid solution 15～25min,
Go sulfuric acid, add after deionized water cleans seed 4-5 time repeatedly and remove deionized water, then seed is soaked in the ethanol solution of 45%
20～40min, remove ethanol, add deionized water and clean seed 3～5 times.
Preferably, described in step (3), soak time is 6-12 hour.
Preferably, described in step (3), germination temperature is 30～33 DEG C.
Preferably, nutritional solution described in step (5) uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.
Preferably, the method moving on to nutritional solution described in step (5) is: joined by nutritional solution in lighttight container, and container cover is many
Orifice plate, wraps up seedling with sponge strip more than root, inserts in container cover porous plate down by the shoot root system having wrapped up sponge, culture fluid
Liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation
Preferably, the composition of basal medium be Armillaria mellea fermented solution 0.65mg/L, Ramulus et folium taxi cuspidatae extracting solution 0.13mg/L, maltose 10g/L,
Agar 3g/L.
The Folium Salicis Babylonicae Herba Verbenae breeding method of the present invention, it is possible to achieve:
1) use through germination, low temperature sleep process, transplant strong sprout and Solution culture method seedling each and every one stage several, finally cultivate seedling.
Having method simple to operation, the seedling time is relatively much shorter, specification consistency advantages of higher.
2) nutritional solution used by and conventional nutraceutical liquid phase ratio, greatly reduce the consumption of compound, decreases the pollution that nutritional solution produces,
Nutritional solution is easily recycled, and the amino acids material and the Chinese medicine class material that add in nutritional solution create synergism, compensate for chemical classes chemical combination
Thing consumption is not enough, reduces pollution simultaneously, improves seed viability, shortens germinating time.
3) extract solution of bamboo leaves of appropriate amount is added, it is possible to promoting germination, the addition of organic selenium improves the nutritional labeling of nutritional solution,
It also is able to promote germination.
Detailed description of the invention
Being embodied as the following detailed description of the present invention, it is necessary to it is pointed out here that, below implement to be only intended to the present invention furtherly
Bright, it is impossible to be interpreted as limiting the scope of the invention, this art skilled person make the present invention according to the invention described above content
Some nonessential improvement and adjustment, still fall within protection scope of the present invention.
The invention provides a kind of Folium Salicis Babylonicae Herba Verbenae breeding method, comprise the steps:
(1) pre-treatment step: disease-free Folium Salicis Babylonicae Herba Verbenae seed is soaked 48h with deionized water under conditions of room temperature and cleans up
After, under sunlight condition, dry in the sun 1-3d, then carries out disinfection to seed, and described sterilization method is: existed by the seed going out decontamination
40% sulfuric acid solution soaks 15～25min, goes sulfuric acid, add after deionized water cleans seed 4-5 time repeatedly and remove deionized water, then
Seed is soaked in the ethanol solution of 45% 20～40min, removes ethanol, add deionized water and clean seed 3～5 times, wherein
Seed can select the minimum Folium Salicis Babylonicae Herba Verbenae seed of seed of Germany's import；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, is being placed in
5-10d in the environment of 10 DEG C；
(3) in the germination stage: step (2) gained seed is placed in deionized water immersion, soak time is 6-12 hour, then
Taking out and cover its surface with wet cloth, treat germination, arrange constant germination temperature, germination temperature is 30～33 DEG C, aseptic
Under conditions of every day in the set time, seed is sprayed water, under sterile conditions, bud point is cut, by bud point move into have basis training
Support base container in carry out basis cultivation, wherein the composition of basal medium be Armillaria mellea fermented solution 0.65mg/L, Ramulus et folium taxi cuspidatae extracting solution
0.13mg/L, maltose 10g/L, agar 3g/L；
(4) transplant the stage in strong sprout: seed detoxic seedling is carried out expanding propagation cultivation, seed detoxic seedling is cultivated in expanding propagation culture medium,
After germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is quartz sand；
Wherein the formula constituent of the expanding propagation culture medium of seed detoxic seedling is: glutamic acid 1.1mg/L, serine mg/L, glycine 1.5mg/L,
Organic selenium 0.9mg/L, riboflavin 0.6mg/L, tapioca starch 0.2mg/L, calcitriol 90mg/L, nicotinic acid 0.2mg/L, seven water sulphuric acid
Ferrous 0.2mg/L；
(5) in the Solution culture method stage: when growth of seedling to two panels true leaf, move on to nutritional solution normal growth from quartz sand or carry out big
Field is planted；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Wherein nutritional solution uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.The method moving on to nutritional solution is: by nutritional solution
Joining in lighttight container, container cover is porous plate, is wrapped up with sponge strip by seedling, will wrap up the shoot root of sponge more than root
System inserts in container cover porous plate down, and culture fluid liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation.
Comparative example 1
Change nutrient composition and carry out Experimental comparison's (without extract solution of bamboo leaves):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 92% reaches 5 leaves.
Comparative example 2
Change nutrient composition and carry out Experimental comparison's (without aminoacid ingredient):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 89% reaches 5 leaves.
Comparative example 3
Change nutrient composition and carry out Experimental comparison's (without Chinese medicine class extracting solution):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 88% reaches 5 leaves.
Without vitamin B5 or organic selenium in the most each embodiment, Folium Salicis Babylonicae Herba Verbenae Seedling respectively more than 90% reaches 5 leaves, and 92
More than % reaches 5 leaves.
Although for illustrative purposes, it has been described that the illustrative embodiments of the present invention, it will be understood by those of skill in the art that not
In the case of departing from the scope and spirit of the invention disclosed in claims, various amendment, interpolation can be carried out in form and details
With the change of replacement etc., and all these change all should belong to the protection domain of claims of the present invention, and claimed
Each department of product and method in each step, can combine with form in any combination.Therefore, public to institute in the present invention
The description of the embodiment opened is not intended to limit the scope of the present invention, but is used for describing the present invention.Correspondingly, the scope of the present invention is not
Limited by embodiment of above, but be defined by claim or its equivalent.
Claims (7)
1. a Folium Salicis Babylonicae Herba Verbenae breeding method, it is characterised in that: comprise the steps:
(1) pre-treatment step: after being cleaned up with deionized water immersion 48h under conditions of room temperature by disease-free Folium Salicis Babylonicae Herba Verbenae seed, dry in the sun 1-3d, then carries out disinfection to seed under sunlight condition；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, 5-10d in the environment of being placed in 10 DEG C；
(3) the germination stage: step (2) gained seed is placed in deionized water immersion, soak time is 6-12 hour, then take out and cover its surface with wet cloth, treating germination, arrange constant germination temperature, germination temperature is 30～33 DEG C, in the set time, seed is sprayed water every day under sterile conditions, under sterile conditions, bud point is cut, the immigration of bud point is had in the container of basal medium and carries out basis cultivation；
(4) transplant the stage in strong sprout: seed detoxic seedling is carried out expanding propagation cultivation, seed detoxic seedling is cultivated in expanding propagation culture medium, after germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is quartz sand；
(5) in the Solution culture method stage: when growth of seedling to two panels true leaf, move on to nutritional solution normal growth from quartz sand or carry out field planting；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
。
2. breeding method as claimed in claim 1, it is characterized in that: described in step (1), sterilization method is: the seed going out decontamination is soaked 15～25min in 40% sulfuric acid solution, go sulfuric acid, add after deionized water cleans seed 4-5 time repeatedly and remove deionized water, seed is soaked in the ethanol solution of 45% 20～40min again, remove ethanol, add deionized water and clean seed 3～5 times.
3. breeding method as claimed in claim 1, it is characterised in that: described in step (3), soak time is 6-12 hour.
4. breeding method as claimed in claim 1, it is characterised in that: described in step (3), germination temperature is 30～33 DEG C.
5. breeding method as claimed in claim 1, it is characterised in that: nutritional solution described in step (5) uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.
6. breeding method as claimed in claim 1, it is characterized in that: the method moving on to nutritional solution described in step (5) is: joined by nutritional solution in lighttight container, container cover is porous plate, seedling is wrapped up with sponge strip more than root, the shoot root system having wrapped up sponge is inserted in container cover porous plate down, culture fluid liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation.
7. breeding method as claimed in claim 1, it is characterised in that: the composition of basal medium is Armillaria mellea fermented solution 0.65mg/L, Ramulus et folium taxi cuspidatae extracting solution 0.13mg/L, maltose 10g/L, agar 3g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610208072.4A CN105850729A (en) | 2016-04-06 | 2016-04-06 | Verbena bonariensis cultivation method |
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| CN201610208072.4A CN105850729A (en) | 2016-04-06 | 2016-04-06 | Verbena bonariensis cultivation method |
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| CN201610208072.4A Pending CN105850729A (en) | 2016-04-06 | 2016-04-06 | Verbena bonariensis cultivation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168408A (en) * | 2018-09-13 | 2019-01-11 | 内蒙古蒙草草种业有限公司 | A method of improving willow leaf Verbena officinalis percentage of seedgermination |
| CN109275555A (en) * | 2018-09-21 | 2019-01-29 | 广西三江县蓝天养殖开发有限公司 | A kind of non-soil culture method of bigleafbeautyberry root or leaf |
| CN111657148A (en) * | 2020-07-07 | 2020-09-15 | 中国热带农业科学院热带作物品种资源研究所 | Grass seed detoxification treatment method |
| CN112931077A (en) * | 2021-01-30 | 2021-06-11 | 安徽济人药业有限公司 | Propagation method for changing wild verbena into family species |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104255510A (en) * | 2014-10-14 | 2015-01-07 | 南京帝道农业科技有限公司 | Rapid propagation method for mutation breeding of herba verbena officinalis |
| CN105052472A (en) * | 2015-08-03 | 2015-11-18 | 陈宝娟 | A high-quality high-yield planting method for organic bananas |
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2016
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255510A (en) * | 2014-10-14 | 2015-01-07 | 南京帝道农业科技有限公司 | Rapid propagation method for mutation breeding of herba verbena officinalis |
| CN105052472A (en) * | 2015-08-03 | 2015-11-18 | 陈宝娟 | A high-quality high-yield planting method for organic bananas |
Non-Patent Citations (2)
| Title |
|---|
| 刘拴成等: "微生物发酵液对九月菊扦插生根的影响", 《内蒙古农业科技》 * |
| 陈华玲等: "柳叶马鞭草繁殖技术研究初报", 《江西农业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168408A (en) * | 2018-09-13 | 2019-01-11 | 内蒙古蒙草草种业有限公司 | A method of improving willow leaf Verbena officinalis percentage of seedgermination |
| CN109275555A (en) * | 2018-09-21 | 2019-01-29 | 广西三江县蓝天养殖开发有限公司 | A kind of non-soil culture method of bigleafbeautyberry root or leaf |
| CN111657148A (en) * | 2020-07-07 | 2020-09-15 | 中国热带农业科学院热带作物品种资源研究所 | Grass seed detoxification treatment method |
| CN112931077A (en) * | 2021-01-30 | 2021-06-11 | 安徽济人药业有限公司 | Propagation method for changing wild verbena into family species |
| CN112931077B (en) * | 2021-01-30 | 2023-06-27 | 安徽济人药业股份有限公司 | Propagation method for wild vervain changing into family species |
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