CN106212229B - A kind of mating system of the extremely resistance to bolting radish germplasm in Shandong District - Google Patents
A kind of mating system of the extremely resistance to bolting radish germplasm in Shandong District Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention discloses a kind of mating systems of the extremely resistance to bolting radish germplasm in Shandong District.The vernalization time of the extremely germplasm of resistance to bolting breeding is adjusted to the October of the previous year by this method (1), to meet the requirement long to light intensity and light of extremely resistance to bolting radish；(2) seed washs repeatedly through sterile water, Da Kening medicament rapid soaking is handled and the water management of vernalization phase, solves the problems, such as that sprout caused by vernalization overlong time is rotted；(3) matrix used can promote side shoot skin zone directly to take root for plain boiled water tongue fur peat+bagasse, shorten transplanting seedling time, and guarantee smoothly harvest seed；(4) breeding is carried out by the radish side shoots at different levels of later period cuttage length 6cm or more in culturing room, it ensure that the quantity of environment Modulatory character and side shoot, and by the adjustment and accurate control to temperature, illumination, light length, liquid manure etc., the extremely germplasm of resistance to bolting is made to smoothly complete reproductive growth acquisition seed.The survival rate of plant of this method is high, and seed quality is good, and heritability is stablized.
Description
Technical field
The invention belongs to plant breeding technique fields, and in particular to a kind of breeding of the extremely resistance to bolting radish germplasm in Shandong District
Method.
Background technique
Radish is the important vegetable crop in China, and spring radish growth momentum is good in recent years, and the market price is high, cultivated area
Grow steadily.But there is also many limiting factors for the spring radish production in China, wherein the most outstanding is earlier bolting, it is earlier to take out
A kind of sedge brings significant impact to the yield and quality of radish, is the bottleneck problem for limiting radish production and annual supply.Screen resistance to pumping
A kind of sedge radish germplasm cultivates suitable resistance to bolting kind, can solve earlier bolting and gives radish production and supply bring heavy losses.
For radish, it is desirable that new varieties are greatly improved in terms of bolting resistant property shape, this depends on grasped trailing plants
Foretell the quality and quantity of germ plasm resource.Therefore it collects extensively, it is rationally resistance to pumping using resistance to bolting radish germ plasm resource that science, which saves,
The important foundation of a kind of sedge radish breeding work.Research of the Li Xixiang etc. (2014) in radish germ plasm resource bolting resistant property characterization and evaluation
In, show that radish is divided into according to resistance to draw: intolerant to bolting germplasm, the germplasm of resistance to bolting, the extremely germplasm of resistance to bolting.
Shandong District, the germplasm of resistance to bolting can by be appropriately extended the seed low temperature vernalization time or spray the method for hormone come
Smoothly obtain seed.However, the requirement of the conditions such as extremely resistance to bolting kind confrontation temperature, intensity of illumination, lighting delay number and hormone is very high, on
Transformation of the nutrient growth to reproductive growth cannot be completed by stating method processing, be unable to get seed.As shown in Figure 1, in the resistance to pumping of tradition
Under a kind of sedge breeding mode, in April, the material of resistance to bolting bloom by bolting, and extremely the material of resistance to bolting does not show flower bud yet.The above problem is to resource
Save, exploitation, benefit be used to say that it is fatal.It does not take effective measures, losing for some extremely resistance to bolting radish germ plasm resources will be caused
It loses.
There is FAQs in the extremely germplasm of the resistance to bolting breeding of Shandong: the extension sprout vernalization time easily causes sprouting stage to rot, can not
It survives；Nutrient growth overlong time, which causes to enter the kind strain of reproductive growth later period, easily catches an illness rotten, can not harvest seed；Extremely resistance to bolting
Characteristic leads to florescence positive value high temperature, and bud aetiolation is serious, can not harvest seed；Extremely resistance to bolting characteristic leads to the later period of bearing pods
Phenomena such as in positive value rainy season, Yi Fasheng seed is underdone rotten, seed can not be harvested.
The method that radish uses lateral branch cutting is the one kind for solving to cause material to be lost due to disease during radish is reserved seed for planting
New method.Applicant had developed a kind of spring white radishes of resistance to bolting lateral branch cutting breeding method (patent No.: ZL in 2014
2014 10504696.1), long using side shoot when this method encounters germ in resistance to bolting radish breeding process and infects fleshy root
It spends 20-30cm, stem thickness 0.5-1cm, 1 grade of side shoot with 3-6 piece leaf and carries out cuttage acquisition seed.But it this method and is not suitable for
In the breeding of extremely resistance to bolting radish germplasm, this is because the requirement of above-mentioned cuttage side shoot is harsher, if being applied to extremely resistance to
It needs to solve there are also many problems in the breeding of bolting germplasm.
Summary of the invention
In order to solve the problems, such as that the breeding difficulty of the Shandong District extremely germplasm of resistance to bolting, the present invention targetedly have adjusted extremely resistance to bolting
The arrangement of time in germplasm breeding period improves seed low temperature vernalization time and method, and numerous using lateral branch cutting completion
The environment conditioning in kind later period, establishes a kind of mating system of extremely resistance to bolting radish varieties in Shandong District, it is ensured that can smoothly receive
Obtain seed.
The technical scheme is that a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District, characterized in that
(1) vernalization treatment
10-15 days in October, the 1st, radish seed is impregnated 10-15 seconds up to gram peaceful 200-300 times of liquid, in 25 DEG C of perseverances
Warm vernalization 24-48 hours, until Seed sprouting；Then vernalization treatment 25-30 days in 2~4 DEG C of refrigerators；Vernalization treatment period needs
Carry out water management.
(2) sprout is planted
After vernalization treatment, sprigging is planted in the kind furrow in greenhouse；
(3) HORMONE TREATMENT
The radish small 6 leaf phase of strain, growing point is sprayed with Exogenous gibberellic acid (GA3) solution, was sprayed again every 7 days primary；
(4) lateral branch cutting in culturing room
Month next year 4-5, the topping when the major branch of kind of strain height reaches 35-40cm；It is reached to 90% or more kind of strain primary lateral branches
Cuttage when to 6cm；
The preparation of substrate soil: counting by volume, plain boiled water tongue fur peat: bagasse=8:2；
The preparation of cuttage alms bowl: substrate soil is packed into nutritive cube, substrate soil is irrigated with water after cuttage noon before that day 15:00
It is spare；
Cuttage branch selects: the disease-free whole side shoots of the stalwartness of side shoot length 6cm or more on plant are cut as cuttage
Side shoot is used for breeding；I.e. all using lateral branch cutting breeding, plant breeding itself is not used；
Processing and cuttage: will select blade of the length greater than 4cm on side shoot to cut from base portion, and top bud length is small
Remove in the whole of 0.5cm；Cuttage is carried out after processing, cuttage is deep-controlled in 3-5cm；
Cuttage branch is placed: nutritive cube being put into seedlings nursing plate, seedlings nursing plate is placed on culturing rack, and culturing rack is all around around no drop
Film；White light dedicated growth lamp is installed above culturing rack；
(5) domestication survives
First week after cuttage, growth lamp is closed, and is sprayed 3-4 times daily, and culture room temperature control is relatively wet at 20-25 DEG C
Degree control is in 65-70%；
After young leaves is sprouted, (opened within 24 hours) using 5000lux intensity illumination, after cuttage 20-25 days, intensity illumination adjustment
To 7000lux；During this period, it is sprayed daily 1 time, water is added in seedlings nursing plate 3 times within every 6-9 days (adding water 1 time within i.e. every 2-3 days)+
Nitrogen-phosphorus-potassium compound fertilizer 1 time (with the 3rd time plus water application)；Other manage same conventional method；
(6) lateral branch cutting breeding
30-35 days after cuttage, the above side shoot of three-level mitogenetic on cuttage side shoot is all removed, florescence artificial pollination, is contained
Florescence terminates, the kind pod color expanded from green to yellow when stop watering, open film, complete after-ripening.
It requires before step (1) radish seed impregnates in Da Kening solution and after impregnating with aseptic water washing kind
Son.
The vernalization of the step (1) are as follows: seed is placed on the culture dish for being lined with filter paper, and adds water to control in seed height
The 1/3-1/4 of degree carries out vernalization；Culture dish is sealed into further vernalization with sealed membrane after the completion of vernalization.
The water management of the step (1) are as follows: every 7 days opening culture dishes are sealed with sealed membrane again after keeping the skin wet.First
Control is added water to when secondary sealing, in culture dish in the 1/4-1/5 of seed height；For the second time when primary sealing to the end, culture dish
Inside adding water to control and drenching but not flow out in filter paper is advisable.
The wide 50-60cm of kind furrow of the step (2) walks road width 30-35cm, the high 10-15cm in aisle, uniline plantation, spacing in the rows
30cm, Routine Management.
The Exogenous gibberellic acid (GA3) that the step (3) is 550mg/L with concentration sprays growing point, spraying time 16:00
After.
The nitrogen of step (5) nitrogen-phosphorus-potassium compound fertilizer, phosphorus, potassium three essential fertilizer ratio are 14:10:18, and when dissolved in water presses
3000-3500 times dilutes use.
The beneficial effects of the present invention are:
1, the present invention targetedly has adjusted the arrangement of time in the extremely germplasm of resistance to bolting breeding period, by the germplasm of resistance to bolting current year
February vernalization is adjusted to the October of the previous year, to meet the requirement long to light intensity and light of extremely resistance to bolting radish.
2, seed of the invention washs repeatedly through sterile water, Da Kening medicament rapid soaking is handled and the water of vernalization phase
It is in charge of reason, solves the problems, such as that sprout caused by vernalization overlong time is rotted, both ensure that the duration of seed vernalization, and in turn ensured bud
The survival rate that seed is planted.
3, the matrix that the present invention uses is plain boiled water tongue fur peat+bagasse, and characteristic is root induction, is retained and breathes freely, can promote
Side shoot skin zone directly takes root, and shortens transplanting seedling time；This matrix nutrition is abundant, it is ensured that smoothly harvests seed.In the present invention, length
The radish lateral branch cuttings at different levels of 6cm or more can take root, and survival rate >=94%, substantially increase the quantity of cuttage side shoot,
And then the acquisition quantity of resistance to bolting radish germplasm can be improved, have great importance.
4, the present invention ensure that environment Modulatory character, and by temperature by later period lateral branch cutting breeding in culturing room
The adjustment of degree, illumination, light length, liquid manure etc. and accurate control, make the extremely germplasm of resistance to bolting smoothly complete reproductive growth, obtain seed.
It is time saving and energy saving without transplanting whole kind strain simultaneously by the way of lateral branch cutting.
5, convenient and efficient of the present invention, it is easy to operate easy to learn.It is survived using the plant that the method for the present invention lateral branch cutting is cultivated
Rate is high, and heritability is stablized.
Detailed description of the invention
Fig. 1 is the chamber planting figure of the material of resistance to bolting and the extremely material of resistance to bolting under traditional mode, as can be seen from Figure 1: secondary
In April in year, the material of resistance to bolting of processing bloom (two row of right side) by bolting, and the extremely material of resistance to bolting of processing does not show flower bud (left side yet
A line).
Fig. 2 is that the domestication of culturing room's side shoot survives figure, as can be seen from Figure 2: using the different size of the method for the present invention cuttage
Side shoot can be tamed and be survived；
Fig. 3 is culturing room's lateral branch cutting breeding figure, as can be seen from Figure 3: using the different size of the method for the present invention cuttage
Side shoot, it is viable and obtain seed.
Specific embodiment
The mating system of the extremely resistance to bolting radish varieties in Shandong District of the invention is carried out combined with specific embodiments below detailed
Thin to illustrate, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thus to protection of the invention
Range is more clearly defined.
Embodiment 1 (radish varieties that the present embodiment is selected are the extremely material of resistance to bolting: the positive radish in day No. 14)
(1) vernalization treatment
Seed pure water flushing 2 times was immersed in up to 10 seconds in gram peaceful 200-300 times of liquid October 10 by First Year,
It is rinsed 1 time with pure water again.Upper filter paper is padded in culture dish, radish seed is placed on wherein, and control is added water in culture dish and is being planted
The 1/3-1/4 of sub- height, 25 DEG C vernalization 32 hours, culture dish is sealed with sealed membrane, the vernalization treatment 25 in 2~4 DEG C of refrigerators
It.Vernalization treatment period needs to carry out water management, specifically: every 7 days opening culture dishes are sealed with sealed membrane again after keeping the skin wet
Mouthful.When sealing for the first time, control is added water in culture dish in the 1/4-1/5 of seed height；For the second time when primary sealing to the end,
Adding water to control in culture dish and drenching but not flow out in filter paper is advisable.
Remarks: Da Kening is produced by Syngenta, 75% Bravo wettable powder, and wide-spectrum bactericide is true to various crop
Fungal disease has preventive and therapeutic effect.
(2) sprout is planted
After vernalization treatment (on November 10 or so), by sprigging in the kind furrow in greenhouse.The kind wide 50cm of furrow, aisle
Wide 30cm, high 10cm.Uniline plantation, spacing in the rows 30cm.Routine Management.
(3) HORMONE TREATMENT
Radish small 6 leaf phase of strain, the Exogenous gibberellic acid for being 550mg/L with concentration (GA3) spray growing point, and spraying time is
After 16:00, sprayed again every 7 days primary.
(4) lateral branch cutting in culturing room
It pinches when planting the major branch height 40cm of strain April 15 next year, Topping Time is 16:00 in afternoon.April 30,
90% or more primary lateral branches carry out cuttage when reaching 6cm or more.
The preparation of substrate soil: counting by volume, plain boiled water tongue fur peat: bagasse=8:2.Wherein, plain boiled water tongue fur peat selects Germany
Klasmann-Deilmann TS1；Its partial size≤5mm, bulk density 0.12-0.14g/cm3, total porosity 45-55%, water holding gap
40-50%, pH value 6.0-6.5, EC value are 0.3-0.5.The commercially available packed bagasse of bagasse selection particle size range 1-5mm.
Cuttage alms bowl prepares: substrate soil is packed into the nutritive cube of 10*10*9cm, base after cuttage noon before that day 15:00
Matter soil is irrigated spare with water.
Cuttage branch selects: take the healthy and strong disease-free side shoots at different levels of side shoot length 6cm or more on plant as cuttage side shoot,
Middle stem thickness is greater than 0.5cm and has the cutting experiment of the cyclic annular side shoot expanded best close to axil end, tames more general at live time
Logical side shoot can shorten 10% or more.
Processing and cuttage: will select side shoot blade length cutting from base portion greater than 4cm, and top bud length is small
In all being removed with ophthalmic tweezers for 0.5cm.Cuttage is carried out after processing, deep-controlled cuttage is 3-5cm.
Cuttage branch is placed: nutritive cube being put into the seedlings nursing plate of 40*60*10cm, seedlings nursing plate is placed on 50*140*90cm training
It supports on frame.Culturing rack is all around around fogging-resistance film.10W LED white light dedicated growth lamp is installed, intensity of illumination above culturing rack:
3000lux-5000lux-7000lux is adjustable.Visible attached drawing 2 of the invention is specifically set.
(5) domestication survives
First week after cuttage, growth lamp is closed, and is sprayed 3-4 times daily.Room temperature is cultivated to control at 20-25 DEG C, it is relatively wet
Degree control is in 65-70%.
Young leaves is sprouted after a week, selects 5000lux intensity illumination (opening within 24 hours), after cuttage 25 days, intensity illumination tune
It is whole to arrive 7000lux.It is sprayed 1 time daily during this period；Every 6-9 days in seedlings nursing plate plus the+NPK compound fertilizer 1 time of water 3 times is (with the 3rd time
Water is added to apply), wherein NPK compound fertilizer (three essential fertilizer ratio 14:10:18) is diluted to 3000-3500 times of aqueous solution application, nursery
Amount of water 1500ml in disk, other manage same conventional method.
As shown in Fig. 2, can tame and survive using the different size side shoot of the method for the present invention cuttage.
(6) lateral branch cutting breeding
32 days after cuttage, the above side shoot of three-level mitogenetic on cuttage side shoot is all removed, florescence artificial pollination, full-bloom stage
Terminate, the kind pod color expanded from green to yellow when, that is, stop watering, open film, complete after-ripening.
As described in Figure 3, viable and obtain seed using the different size side shoot of the method for the present invention cuttage, it is detailed in table
1。
The radish varieties that embodiment 2:(the present embodiment is selected are the extremely material of resistance to bolting: late ㄆ G)
The present embodiment step (1) vernalization treatment 28 days in 2~4 DEG C of refrigerators, 25 DEG C vernalization 38 hours, remaining same embodiment
1。
It pinches when planting the major branch height 36cm of strain the present embodiment step (4) 29 days April next year.Planted strain May 17
90% or more primary lateral branches carry out cuttage when reaching 6cm or more.Remaining is the same as embodiment 1.The results are shown in Table 1 for breeding.
The radish varieties that embodiment 3:(the present embodiment is selected are the extremely material of resistance to bolting: the snow sword spring)
The present embodiment step (1) vernalization treatment 28 days in 2~4 DEG C of refrigerators, 25 DEG C vernalization 42 hours, remaining same embodiment
1。
It pinches when planting the major branch height 36cm of strain the present embodiment step (4) 19 days April next year.Planted strain May 8
90% or more primary lateral branches carry out cuttage when reaching 6cm or more.Remaining is the same as embodiment 1.The results are shown in Table 1 for breeding.
The extremely material of the resistance to bolting breeding situation of table 1
As can be seen from Table 1: cuttage survival rate >=94.0% of the invention, side shoot, which averagely bears pods, counts >=38.8, and plants
High-quality, the germination percentage 100% of son.This absolutely proves that method of the invention is the extremely resistance to bolting radish germplasm in Shandong District
A kind of practicable mating system, to the preservation of germ plasm resource, exploitation, using having great importance.
Above-described embodiment is not limit the invention in any way, all to be obtained by the way of equivalent substitution or equivalent transformation
Technical solution fall within the scope of protection of the present invention.
Claims (9)
1. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District, characterized in that
(1) vernalization treatment
10-15 days in October, the 1st, will extremely be impregnated 10-15 seconds in reaching gram peaceful 200-300 times of liquid the radish seed of resistance to bolting, in
25 DEG C constant temperature vernalization 24-48 hours, until Seed sprouting；Then vernalization treatment 25-30 days in 2~4 DEG C of refrigerators；When vernalization treatment
Phase needs to carry out water management；
(2) sprout is planted
After vernalization treatment, sprigging is planted in the kind furrow in greenhouse；
(3) HORMONE TREATMENT
The radish small 6 leaf phase of strain, growing point is sprayed with Exogenous gibberellic acid solution, was sprayed again every 7 days primary；
(4) lateral branch cutting in culturing room
It pinches when the major branch of kind of strain height reaches 35-40cm month next year 4-5；It is reached to 90% or more kind of strain primary lateral branches
Cuttage when to 6cm；
The preparation of substrate soil: counting by volume, plain boiled water tongue fur peat: bagasse=8:2；
The preparation of cuttage alms bowl: substrate soil is packed into nutritive cube, substrate soil is irrigated standby with water after cuttage noon before that day 15:00
With；
Cuttage branch selects: the disease-free whole side shoots of the stalwartness of side shoot length 6cm or more on plant cut as cuttage side shoot,
For breeding；
Processing and cuttage: blade of the length greater than 4cm on side shoot will have been selected to cut from base portion, top bud length has been less than
The whole of 0.5cm is removed；Cuttage is carried out after processing, cuttage is deep-controlled in 3-5cm；
Cuttage branch is placed: nutritive cube being put into seedlings nursing plate, seedlings nursing plate is placed on culturing rack, and culturing rack is all around around thin without dripping
Film；White light dedicated growth lamp is installed above culturing rack；
(5) domestication survives
First week after cuttage, growth lamp is closed, and by spraying 3-4 time daily, culture room temperature controls the relative humidity control at 20-25 DEG C
System is in 65-70%；
After young leaves is sprouted, using 5000lux intensity illumination, after cuttage 20-25 days, intensity illumination is adjusted to 7000lux；In this phase
In, it is sprayed 1 time, adds 3 times+nitrogen-phosphorus-potassium compound fertilizer of water 1 time in seedlings nursing plate daily within every 6-9 days；Nitrogen-phosphorus-potassium compound fertilizer is with the 3rd time
Water is added to apply；It opens within above-mentioned strong light illumination equal 24 hours；
(6) lateral branch cutting breeding
30-35 days after cuttage, the above side shoot of three-level mitogenetic on cuttage side shoot is all removed, florescence artificial pollination, full-bloom stage
Terminate, the kind pod color expanded from green to yellow when stop watering, open film, complete after-ripening.
2. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as described in claim 1, characterized in that the step
Suddenly the vernalization of (1) are as follows: seed is placed on the culture dish for being lined with filter paper, and add water to control seed height 1/3-1/4 into
Row vernalization；Culture dish is sealed into further vernalization with sealed membrane after the completion of vernalization.
3. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as claimed in claim 2, characterized in that the step
Suddenly the water management of (1) are as follows: every 7 days openings culture dish sealed membrane seals again after keeping the skin wet；When sealing for the first time, in culture dish
Control is added water in the 1/4-1/5 of seed height；For the second time when primary sealing to the end, control is added water in culture dish in filter paper
Drenched but not outflow.
4. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as described in claim 1, characterized in that the step
Suddenly it requires before (1) radish seed impregnates in Da Kening solution and after impregnating with aseptic water washing seed.
5. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as described in claim 1, characterized in that the step
Suddenly the wide 50-60cm of kind furrow of (2), uniline plantation, spacing in the rows 30cm.
6. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as described in claim 1, characterized in that the step
Suddenly (3) spray growing point with the gibberellin that concentration is 550mg/L.
7. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as claimed in claim 6, characterized in that described red
After mycin spraying time is 16:00.
8. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as described in claim 1, characterized in that the step
Suddenly the nitrogen of (5) nitrogen-phosphorus-potassium compound fertilizer, phosphorus, potassium three essential fertilizer ratio are 14:10:18.
9. a kind of mating system of the extremely resistance to bolting radish varieties in Shandong District as claimed in claim 8, characterized in that the nitrogen
Use is diluted by 3000-3500 times when phosphorus potassium complex fertilizer dissolved in water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610585648.9A CN106212229B (en) | 2016-07-22 | 2016-07-22 | A kind of mating system of the extremely resistance to bolting radish germplasm in Shandong District |
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| CN201610585648.9A CN106212229B (en) | 2016-07-22 | 2016-07-22 | A kind of mating system of the extremely resistance to bolting radish germplasm in Shandong District |
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| CN106212229B true CN106212229B (en) | 2019-12-03 |
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