CN105993871A - Rapid seedling-raising method capable of promoting high germination rate of pseudo-ginseng by utilizing acremonium strictum - Google Patents

Rapid seedling-raising method capable of promoting high germination rate of pseudo-ginseng by utilizing acremonium strictum Download PDF

Info

Publication number
CN105993871A
CN105993871A CN201610323271.XA CN201610323271A CN105993871A CN 105993871 A CN105993871 A CN 105993871A CN 201610323271 A CN201610323271 A CN 201610323271A CN 105993871 A CN105993871 A CN 105993871A
Authority
CN
China
Prior art keywords
seedling
germination rate
radix notoginseng
acremonium
spore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610323271.XA
Other languages
Chinese (zh)
Other versions
CN105993871B (en
Inventor
杜云龙
何霞红
万媛媛
张靖
杜丽思
李成云
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Du Yunlong
Yunnan Agricultural University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610323271.XA priority Critical patent/CN105993871B/en
Publication of CN105993871A publication Critical patent/CN105993871A/en
Application granted granted Critical
Publication of CN105993871B publication Critical patent/CN105993871B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a rapid seedling-raising method capable of promoting high germination rate of pseudo-ginseng by utilizing acremonium strictum. The rapid seedling-raising method comprises the following steps: acremonium strictum strain culturing, seed treatment, sowing, breeding management, and germination and transplanting. According to the invention, compared with traditional irrigation of seeds with water, the germination rate of pseudo-ginseng seeds is increased by 20% or more, and can reach up to 90% or more; the time of seedling raising can be shortened by 10 to 20 days; meanwhile, the cultured seedlings are strong and neat and grow rapidly, and the time of seedling raising can be saved, so great significance on plantation of the pseudo-ginseng is obtained.

Description

A kind of fast seedling-cultivating method utilizing an acremonium to promote Radix Notoginseng height germination rate
Technical field
The present invention relates to method for culturing seedlings field, be specifically related to a kind of fast seedling-cultivating method utilizing an acremonium to promote Radix Notoginseng height germination rate.
Background technology
Radix Notoginseng is Araliaceae Panax herbaceos perennial, has " Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) ", a title of " southern part of the country SHENCAO ", its root, stem, spends and all can be used as medicine, and is the distinctive rare Chinese medicine in Yunnan.Pharmaceutical research shows now, and Radix Notoginseng has pharmacologically active and unique curative effect in blood system, cardiovascular system, nervous system, immune system, metabolic system and antiinflammatory, defying age, anti-swollen method.Notoginseng planting method usually seed collecting, preservation, nursery, transplant, gather.The nursery means used at present are that after gathering, the seed that preserves of moisturizing is sowed at soil, under conditions of keeping suitable temperature, moisture content, make Panax notoginseng seeds sprout and obtain seedling.Owing to Radix Notoginseng has obvious allelopathy effect, germination percentage is relatively low, seedling raise period is longer in sowing the most on the spot, the most less employing.In prior art, Radix Notoginseng nursery typically use directly be embedded in humidity substrate seedling and sterilization dry refrigerator cold-storage after be embedded in soil or substrate seedling, although the germination rate of Panax notoginseng seeds all can be improved compared with seeding and seedling raising under natural environment, but there is also germination rate and can only achieve 50～70%, sprouting time is up to 45～60d, seriously constrains the development of notoginseng planting industry.And affected by various extreme weather conditions in recent years so that under the conditions of prior art, Panax notoginseng seeds germination percentage and seedling percent are substantially reduced, therefore study the most quickly improve Panax notoginseng seeds sprout significant.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of fast seedling-cultivating method utilizing an acremonium to promote Radix Notoginseng height germination rate, it is possible to increase the germination rate of Panax notoginseng seeds, shorten seedling raise period.
For realizing object above, the present invention is achieved by the following technical programs:
A kind of fast seedling-cultivating method utilizing an acremonium to promote Radix Notoginseng height germination rate, comprises the following steps:
1) spawn culture: be taken in 28 DEG C of incubators branch acremonium strain (the Acremonium yunnanensis Y.L.Du MHYAU3001 preserved, preserve numbered 12374, entitled Institute of Microorganism, Academia Sinica of depositary institution, depositary institution address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date is on 04 21st, 2016), it is inoculated in PDA culture medium, 28 DEG C of dark culturing 2 weeks, collect mycelia and the spore of growth, add water and stir, the aqueous solution of mycelia and spore must be contained；
2) seed treatment: growth selection is good, Radix Notoginseng plant without pest and disease damage, Radix Notoginseng Red mature fruit of gathering removes peel, obtains Panax notoginseng seeds, sterilizes, ted；
3) sowing: select the Panax notoginseng seeds after the sterilization of full grains, being seeded into humidity is in the seedling medium that 25-30% contains perlite and quartz sand, by step 1) the aqueous solution containing mycelia and spore irrigate, watered once with the aqueous solution containing mycelia and spore every 12-15 days afterwards, repeating to water twice, natural lighting is sprouted；
4) breeding management: after planting keeping the humidity containing perlite with the seedling medium of quartz sand is 25-30%, and temperature is 22-26 DEG C, and illumination is ventilated；
5) transplanting is sprouted: after planting 40-45d Panax notoginseng seeds starts to sprout, and 50-60d Radix Notoginseng Seedling average height is at 5-7cm, and about about 55-65d can transplant.
Preferably, described step 1) mycelia and concentration OD of spore in aqueous solution containing mycelia and spore600For 0.2-0.3.
Preferably, described step 2) in sterilization be use 10% liquor natrii hypochloritis soak Panax notoginseng seeds 8-10min.
Preferably, described step 2) in ted the moisture content of rear Panax notoginseng seeds be 10-12%.
Preferably, described step 3) depth of planting controls at 1.0-1.5cm.
The method have the benefit that the branch acremonium in the present invention not only promotes the rudiment of Panax notoginseng seeds, also promote the growth of Radix Notoginseng seedling, improving the survival rate of seedling, more traditional water pouring seed is compared, and the germination rate of Panax notoginseng seeds improves more than 20%, germination rate can be up to more than 90%, seedling raise period can shorten 10-20 days, and the seedling cultivated is healthy and strong neat, and growth is fast, can save the time of nursery, the plantation tool for Radix Notoginseng is of great significance.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, the accompanying drawing used required in embodiment or description of the prior art will be briefly described below, apparently, accompanying drawing in describing below is only some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Radix Notoginseng growth of seedling situation after Fig. 1: experimental group and matched group sowing 55d, wherein A is matched group, and B is experimental group.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment:
A kind of fast seedling-cultivating method utilizing an acremonium to promote Radix Notoginseng height germination rate, comprises the following steps:
1) spawn culture: be taken in 28 DEG C of incubators branch acremonium strain (the Acremonium yunnanensis Y.L.Du MHYAU3001 preserved, preserve numbered 12374), it is inoculated in PDA culture medium, 28 DEG C of dark culturing 2 weeks, collect mycelia and the spore of growth, add water to stir, obtain concentration OD of mycelia and spore600Aqueous solution for 0.2-0.3；
2) seed treatment: growth selection is good, Radix Notoginseng plant without pest and disease damage, Radix Notoginseng Red mature fruit of gathering removes peel, obtains Panax notoginseng seeds, soaks Panax notoginseng seeds 8-10min with the liquor natrii hypochloritis of 10% and sterilize, and taking-up, tedding and making its moisture content is 10-12%；
3) sowing: select the Panax notoginseng seeds after the sterilization of full grains, being seeded into humidity is in the seedling medium that 25-30% contains perlite and quartz sand, depth of planting is 1.0-1.5cm, by step 1) the aqueous solution containing mycelia and spore irrigate, afterwards every pouring in 12-15 days once, repeating to water twice, natural lighting is sprouted；
4) breeding management: after planting keeping the humidity containing perlite with the seedling medium of quartz sand is 25-30%, and temperature is 22-26 DEG C, and illumination is ventilated.
5) transplanting is sprouted: after planting 40-45d Panax notoginseng seeds starts to sprout, and 50-60d Radix Notoginseng Seedling average height is at 5-7cm, and about about 55-65d can transplant.
Preparation is containing branch acremonium mycelia and spore concentration OD600Being the aqueous solution of 0.25, carry out nursery by the above process as experimental group, additionally matched group uses water to replace branch acremonium after planting to be irrigated by seedling medium in Panax notoginseng seeds sterilization, and remaining nursery step and rearing condition are identical with experimental group.From figure 1 it appears that matched group growth of seedling speed is significantly faster than that matched group, experimental group after planting 40-45d Panax notoginseng seeds starts to sprout, and 50-60d Radix Notoginseng Seedling average height may be up to 90% at 5-7cm, germination rate；Matched group after planting 50-60d Panax notoginseng seeds just starts to sprout, and germination rate meansigma methods only has 71%.
Above example only in order to technical scheme to be described, is not intended to limit；Although the present invention being described in detail with reference to previous embodiment, it will be understood by those within the art that: the technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.

Claims (5)

1. utilize an acremonium to promote a fast seedling-cultivating method for Radix Notoginseng height germination rate, its feature It is, comprises the following steps:
1) spawn culture: be taken in 28 DEG C of incubators the branch acremonium strain preserved (Acremonium yunnanensis Y.L.Du MHYAU3001, preserve numbered 12374), It is inoculated in PDA culture medium, 28 DEG C of dark culturing 2 weeks, collects mycelia and the spore of growth, Add water and stir, the aqueous solution of mycelia and spore must be contained；
2) seed treatment: growth selection is good, Radix Notoginseng plant without pest and disease damage, and Radix Notoginseng of gathering is red Ripe fruit, removes peel, obtains Panax notoginseng seeds, sterilization, teds；
3) sowing: selecting the Panax notoginseng seeds after the sterilization of full grains, being seeded into humidity is In the 25-30% seedling medium containing perlite and quartz sand, by step 1) containing mycelia and spore Aqueous solution irrigate, afterwards every 12-15 days with containing mycelia and spore aqueous solution water once, Repeating to water twice, natural lighting is sprouted；
4) breeding management: after planting holding containing the humidity of perlite with the seedling medium of quartz sand is 25-30%, temperature is 22-26 DEG C, and illumination is ventilated；
5) transplanting is sprouted: after planting 40-45d Panax notoginseng seeds starts to sprout, 50-60d Radix Notoginseng Seedling Average height is at 5-7cm, and about about 55-65d can transplant.
Branch acremonium is utilized to promote quickly educating of Radix Notoginseng height germination rate the most as claimed in claim 1 Seedling-growing method, it is characterised in that described step 1) mycelia and spore in aqueous solution containing mycelia and spore Concentration OD of son600For 0.2-0.3.
Branch acremonium is utilized to promote quickly educating of Radix Notoginseng height germination rate the most as claimed in claim 1 Seedling-growing method, it is characterised in that described step 2) in sterilization be use 10% liquor natrii hypochloritis Soak Panax notoginseng seeds 8-10min.
Branch acremonium is utilized to promote quickly educating of Radix Notoginseng height germination rate the most as claimed in claim 1 Seedling-growing method, it is characterised in that described step 2) in ted the moisture content of rear Panax notoginseng seeds and be 10-12%.
Branch acremonium is utilized to promote quickly educating of Radix Notoginseng height germination rate the most as claimed in claim 1 Seedling-growing method, it is characterised in that described step 3) depth of planting controls at 1.0-1.5cm.
CN201610323271.XA 2016-05-16 2016-05-16 A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium Expired - Fee Related CN105993871B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610323271.XA CN105993871B (en) 2016-05-16 2016-05-16 A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610323271.XA CN105993871B (en) 2016-05-16 2016-05-16 A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium

Publications (2)

Publication Number Publication Date
CN105993871A true CN105993871A (en) 2016-10-12
CN105993871B CN105993871B (en) 2019-10-22

Family

ID=57097408

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610323271.XA Expired - Fee Related CN105993871B (en) 2016-05-16 2016-05-16 A kind of fast seedling-cultivating method promoting the high germination rate of Radix Notoginseng using branch acremonium

Country Status (1)

Country Link
CN (1) CN105993871B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109497091A (en) * 2018-12-27 2019-03-22 云南农业大学 A kind of biological prevention and control agent and preparation method thereof with nematocide function
CN109721403A (en) * 2018-12-29 2019-05-07 云南农业大学 A kind of microbial culture medium and preparation method thereof promoting soybean growth

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101444230A (en) * 2008-12-26 2009-06-03 南京师范大学 Method for producing novel lance atractylodes rhizome inoculation agent
CN103444387A (en) * 2012-05-31 2013-12-18 黄九荣 Panax notoginseng planting method
CN103583108A (en) * 2013-11-01 2014-02-19 云南农业大学 Pseudo-ginseng seedling growing method
CN104447127A (en) * 2014-12-17 2015-03-25 成都新朝阳作物科学有限公司 Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof
CN105191542A (en) * 2015-09-16 2015-12-30 云南三七科技资源药物研究有限公司 High-germination-rate fast seedling growing method for pseudo-ginseng seeds
CN105340708A (en) * 2015-11-10 2016-02-24 云南师范大学 Industrialized seedling production method of panax notoginseng
CN105379611A (en) * 2015-10-14 2016-03-09 中国科学院昆明植物研究所 Rapid propagation method for panax bipinnatifidus seed
CN105462847A (en) * 2015-12-23 2016-04-06 广西大学 Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101444230A (en) * 2008-12-26 2009-06-03 南京师范大学 Method for producing novel lance atractylodes rhizome inoculation agent
CN103444387A (en) * 2012-05-31 2013-12-18 黄九荣 Panax notoginseng planting method
CN103583108A (en) * 2013-11-01 2014-02-19 云南农业大学 Pseudo-ginseng seedling growing method
CN104447127A (en) * 2014-12-17 2015-03-25 成都新朝阳作物科学有限公司 Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof
CN105191542A (en) * 2015-09-16 2015-12-30 云南三七科技资源药物研究有限公司 High-germination-rate fast seedling growing method for pseudo-ginseng seeds
CN105379611A (en) * 2015-10-14 2016-03-09 中国科学院昆明植物研究所 Rapid propagation method for panax bipinnatifidus seed
CN105340708A (en) * 2015-11-10 2016-02-24 云南师范大学 Industrialized seedling production method of panax notoginseng
CN105462847A (en) * 2015-12-23 2016-04-06 广西大学 Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈士林，肖培根主编: "《中药资源可持续利用导论》", 31 March 2006, 北京：中国医药科技出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109497091A (en) * 2018-12-27 2019-03-22 云南农业大学 A kind of biological prevention and control agent and preparation method thereof with nematocide function
CN109497091B (en) * 2018-12-27 2021-04-20 云南农业大学 Biological control preparation with nematode killing function and preparation method thereof
CN109721403A (en) * 2018-12-29 2019-05-07 云南农业大学 A kind of microbial culture medium and preparation method thereof promoting soybean growth

Also Published As

Publication number Publication date
CN105993871B (en) 2019-10-22

Similar Documents

Publication Publication Date Title
CN103430847A (en) Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata
CN105103856A (en) Method for cultivating moringa oleifera suitable in northern area
CN104380994A (en) Bletilla striata large-scale high-yield cultivation method
CN103283457B (en) Drought-resisting batata seedling culture method
CN105123529A (en) Rapid propagation and efficient cultivation method of Bletilla striata
CN104082138A (en) Tissue-culture rapid propagation method of Aristolochia fordiana Hemsl
CN103329802A (en) Pollution-free high-yield planting method for strawberries
CN102696407B (en) Non-tissue-cultured walnut micro-cutting grafting seedling raising method
CN104041412A (en) Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei
CN106358773B (en) The tree-like breeding method of walnut " trunk type "
CN106688801A (en) Leafiness high yield horseradish tree nursery stock cultivating and planting method
CN102057871B (en) Method for breeding cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for north latitude 26 to 30 degrees
CN103371045A (en) Reproduction method for lilium davidii unicoior cotton
CN103070070A (en) Cultivation method of seedless roxburgh roses
CN105660391A (en) Tissue culture breeding method for apple sapling
CN104641879A (en) Method for planting dendrobium candidum in metasequoia tree forest
CN105993871A (en) Rapid seedling-raising method capable of promoting high germination rate of pseudo-ginseng by utilizing acremonium strictum
CN109287461B (en) Tomato seedling cultivation method capable of remarkably improving early-stage yield
CN104303765B (en) The high-yield planting method of the stem of noble dendrobium
CN103975832B (en) A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method
CN102771350B (en) Method for cultivating mycorrhizal seedlings
CN109220478A (en) A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf
CN108450335A (en) A kind of fresh water sand pear stem section tissue rapid propagation method
CN105815104A (en) Four-step method for raising branching rate of transplanted oil-used peony
CN105794650A (en) Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20200106

Address after: 650201 Yunnan Province, Panlong District of Kunming City gold black drop cable slope No. 132

Co-patentee after: Du Yunlong

Patentee after: Yunnan Agricultural University

Address before: 650201, room 1, unit 24, building 666, No. 502, Xiaokang Road, Wuhua District, Yunnan, Kunming

Patentee before: Du Yunlong

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20191022

CF01 Termination of patent right due to non-payment of annual fee