CN109220478A - A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf - Google Patents
A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf Download PDFInfo
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- CN109220478A CN109220478A CN201811281096.8A CN201811281096A CN109220478A CN 109220478 A CN109220478 A CN 109220478A CN 201811281096 A CN201811281096 A CN 201811281096A CN 109220478 A CN109220478 A CN 109220478A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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Abstract
The invention discloses a kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf, including selection of land site preparation, cultivation seedling, transplanting, field management, harvesting.Artificial method for planting of the invention, had not only maintained the drug effect of pilose actinodaphne bark or leaf, but also improved the yield of pilose actinodaphne bark or leaf, met demand of the pharmaceutical market to pilose actinodaphne bark or leaf.
Description
Technical field
The present invention relates to planting technical field, the artificial method for planting of specifically a kind of medicinal pilose actinodaphne bark or leaf.
Background technique
Pilose actinodaphne bark or leaf, [alias] false beautiful osmanthus；[Latin]Celtis philippinensis BlancoIt is grown in mixed friendship
Lin Zhong is distributed in the ground such as Guangdong, Guangxi, Yunnan.Dungarunga or shrub, up to 4-6 meter.Branch has brown villus.Leaf alternate, normal 3
Or 5 close verticillate；Blade leathery, obovate ovalisation is 12-20 centimetres long, and 5-12 centimetres wide, apex is suddenly tapering, and base portion is anxious
There is rufous villus on point, young leaves two sides, and light above old leaf has rust hair below；It is slightly raised above middle arteries and lateral vein, below it is bright
Aobvious protrusion；Petiole is thick, 1.5-3 centimetres long, villous.Flower unisexuality, dioecism；Circular cone locusta axillary；Male inflorescence is 6 lis long
Rice, male flower perianth sliver ellipse is 3 millimeters long, there is long pubescence, stamen 9, the usual stockless of body of gland of 3 pieces of most interior base portions, filigree
There is long pubescence, anther oblong, pistillode is tiny, and column cap 2 is shallowly split；Female inflorescence is 10 centimetres long, the long 1.5-2 of female flower perianth sliver
Millimeter, there is echinid, and there is long pubescence in outside, and staminodium is tiny, and gynoecium has long pubescence, style bending, and column cap 2 is split.Fruit is spherical, diameter
4-6 millimeters, hypocarp plate-like.The month at florescence 8-12.The fruiting period next year 2-3 month.Pilose actinodaphne bark or leaf is used as medicine with root skin, leaf, chief value: for driving
Wind, detumescence, broken product, removing toxic substances, control traumatic injury, sore furuncle poison.
With the excavation of pilose actinodaphne bark or leaf purposes, the demand of pilose actinodaphne bark or leaf is more and more, but the nowadays big portion in source of pilose actinodaphne bark or leaf
Divide and be still only limitted to wild autonomous growth, artificial growth is rarely seen so far to be had been reported that.In order to meet the needs of market is to pilose actinodaphne bark or leaf, it is badly in need of
A kind of artificial method for planting of science promotes the yield of pilose actinodaphne bark or leaf under the premise of keeping pilose actinodaphne bark or leaf drug effect itself.
Summary of the invention
The object of the present invention is to provide a kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf, using the artificial method for planting, both
The drug effect of pilose actinodaphne bark or leaf is maintained, and improves the yield of pilose actinodaphne bark or leaf, meets demand of the pharmaceutical market to pilose actinodaphne bark or leaf.
A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf includes selection of land site preparation, cultivates seedling, transplanting, field management, harvesting,
Particular content is as follows:
(1) it selection of land site preparation: selects to have abundant water resources, the planting site of soil layer is deep, fertile hillside fields in the south as pilose actinodaphne bark or leaf；It is transplanting
Deep plough to soil in month previous year 10-11, depth 45cm-55cm, apply base manure, 250-450 kgs/acre.
(2) it cultivates seedling: choosing the lateral bud of staminiferous plant pilose actinodaphne bark or leaf as explant, cultivated using quick breeding by group culture method fragrant
Bakelite tissue-cultured seedling grows to 5cm-8cm high to it, has 5-8 item root time shift to enter nutrient bag to be cultivated.
(3) it transplants: when seedling long supreme 0.8m-1.0m, hardening 10-20 days, then by its balled transplanting, transplant seeding row spacing
For 1.5m*2.0m, depth 35cm-45cm, seedling is put into kind of plant hole after backfill and irrigates root water, then spreads one above
Layer fine earth；Sick seedling, dead seedling are removed after 15 days in time, and is filled the gaps with seedlings in time.
(4) field management:
1) after transplanting in 6 months, 1%-2% aqueous solution of urea of spraying every month, urea mu dosage 30-40kg；After 6 months every
One month application organic fertilizer, every is applied 1.2-1.5kg；After 1 year, 3 organic fertilizers are applied every year, respectively in May in April-, 8
Month-September part, January next year in December-, 2-3 tons of mu dosage；
2) as the long supreme 1.3m-1.5m of sapling, trunk is cut off, retains 3-5 main side branch, is evenly distributed on the surrounding of trunk；
The height for controlling pilose actinodaphne bark or leaf is no more than 2.5m；It also needs to trim sick branch, deadwood, overstocked branch, crossing branch every year, keeps logical
Wind light transmission reduces disease.
(5) it harvests: annual 7-8 month and 11-12 month, harvesting the leaf of pilose actinodaphne bark or leaf；11-12 month root is excavated, received
Root skin is taken, every pilose actinodaphne bark or leaf at least retains 3 complete main roots.
The base manure is made of following parts by weight raw material: 20-50 parts of cow dung, 50-70 parts of pig manure, fruit/vegetable waste 60-
80 parts, fermentation 1-2 months.
The organic fertilizer is made of following parts by weight raw material: pilose actinodaphne bark or leaf fall leaves 40-60 parts, 40-60 parts of fruit/vegetable waste,
30-60 parts of lignin, 10-30 parts of ferment bacterium, powder 8-10 parts molasses fermented, 6-8 parts of Calusena lansium leaf extract, xanthium sibiricum
6-8 parts, 10-30 parts of bone powder, 10-15 parts of methane tank residue.
The nutrient bag is by two or more mixture in coco bran, thin river sand, perlite, biomass carbon as matrix.
It is described that using quick breeding by group culture method cultivation pilose actinodaphne bark or leaf tissue-cultured seedling, specific step is as follows:
(1) explant is handled: being chosen the lateral bud of staminiferous plant pilose actinodaphne bark or leaf, is cleaned up with clear water, the alcohol that suck dry moisture is put into 75% is molten
10-15s is impregnated in liquid, is impregnated 25-30min with aseptic water washing 3-5 times, then with 2% liquor natrii hypochloritis, is rushed with sterile water
It washes 2-3 times, cleaned lateral bud is clamped with tweezers, on aseptic operating platform, blot surface moisture with the filter paper to sterilize.
(2) Primary culture: the explant after sterilizing being inoculated into the Primary culture base through high-temperature sterilization and is cultivated, and cultivates item
Part is 24-26 DEG C of temperature, intensity of illumination 2000-2500lx, light application time 12-16h/d, and sterile culture is extremely in culturing room
It sprouts.
(3) squamous subculture: moving into the subculture medium through high-temperature sterilization for bud obtained by step (2), is 24- in cultivation temperature
25 DEG C, light intensity 2000-2200lx, light application time cultivate 35-40d under conditions of being 14-16h/d.
(4) culture of rootage: aseptically, squamous subculture is obtained into no offspring, is inserted into root media and is given birth to
Root culture；Cultivation temperature is 26-28 DEG C, light intensity 2000-22001x, light application time 13-15h/d.
The ingredient of Primary culture base are as follows: MS+ N6 benzyladenine 1.2mg/L+0 .6mg/L of methyl α-naphthyl acetate+vitamin B1
1 .0mg/L+molasses 3g/L+ agar 4g/L, culture medium pH value are 6.0；
The ingredient of subculture medium are as follows: MS+ N6 benzyladenine 1.8mg/L+ methyl α-naphthyl acetate 0.5mg/L+ vitamin B1 0.8mg/L
+ vitamin C 1.5mg/L+ glutamic acid 2.1mg/L+ molasses 3.5g/L+ agar 4g/L, culture medium pH value are 5.8；
The ingredient of root media are as follows: 1/2MS++methyl α-naphthyl acetate 0.2mg/L+ indolebutyric acid 0.8mg/L+ heteroauxin 0.2mg/L+
Molasses 4.5g/L+ agar 4g/L, culture medium pH value are 6.1.
The beneficial effects of the present invention are:
The present invention cultivates pilose actinodaphne bark or leaf tissue-cultured seedling using quick breeding by group culture method, then moves it into nutrient bag to continue to cultivate and obtain
The seedling of pilose actinodaphne bark or leaf, cultivating seedling by this method, not only material is easy to get, pilose actinodaphne bark or leaf raw material dosage children that is few, but also cultivating
Seedling well developed root system, virus less, be able to maintain the medicinal characteristic and environmental suitability of wild pilose actinodaphne bark or leaf.Pilose actinodaphne bark or leaf belongs to dioecism plant
Object, select staminiferous plant pilose actinodaphne bark or leaf lateral bud as explant, the pilose actinodaphne bark or leaf seedling cultivated can be made to be all staminiferous plant, nothing as a result,
Nutritional ingredient is mainly used for growing leaf and rhizome, improves the yield of leaf and root skin.
In the present invention, only application of organic fertilizers can influence drug effect to avoid fertilizer residue；Wampee is added in organic fertilizer
Leaf extract and xanthium sibiricum, can effectively expelling parasite, reduction disease.Using artificial method for planting of the invention, both kept
The drug effect of pilose actinodaphne bark or leaf, and the yield of pilose actinodaphne bark or leaf is improved, meet demand of the pharmaceutical market to pilose actinodaphne bark or leaf.
Specific embodiment
For the more detailed introduction present invention, below with reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf includes selection of land site preparation, cultivates seedling, transplanting, field management, harvesting, specifically
Content is as follows:
(1) selection of land site preparation: in the hillside fields in the south that Guangxi Daxin County selects to have abundant water resources, soil layer is deep, fertile as pilose actinodaphne bark or leaf
Planting site；It deep ploughs in November previous year of transplanting to soil, depth 45cm-55cm, application base manure, 350 kilograms/
Mu.
(2) it cultivates seedling: choosing the lateral bud of staminiferous plant pilose actinodaphne bark or leaf as explant, cultivated using quick breeding by group culture method fragrant
Bakelite tissue-cultured seedling grows to 5cm-8cm high to it, has 5-8 item root time shift to enter nutrient bag to be cultivated.
(3) it transplants: when seedling long supreme 0.8m-1.0m, hardening 15 days, then by its balled transplanting, transplanting seeding row spacing is
1.5m*2.0m, depth 35cm-45cm are put into seedling in kind of plant hole and irrigate root water after backfill, then are spreading one layer above
Fine earth；Sick seedling, dead seedling are removed after 15 days in time, and is filled the gaps with seedlings in time.
(4) field management:
1) after transplanting in 6 months, 1% aqueous solution of urea of spraying every month, urea mu dosage 30kg；After 6 months every other month
Organic fertilizer is applied, every is applied 1.5kg；After 1 year, 3 organic fertilizers are applied every year, and respectively in April, August part, December, mu is used
2.5 tons of amount；
2) as the long supreme 1.3m-1.5m of sapling, trunk is cut off, retains 3-5 main side branch, is evenly distributed on the surrounding of trunk；
The height for controlling pilose actinodaphne bark or leaf is no more than 2.5m；It also needs to trim sick branch, deadwood, overstocked branch, crossing branch every year, keeps logical
Wind light transmission reduces disease.
(5) it harvests: annual July and November, harvesting the leaf of pilose actinodaphne bark or leaf；Root is excavated, root skin is collected November,
Each tree at least retains 3 complete main roots.
The base manure is made of following parts by weight raw material: 35 parts of cow dung, 60 parts of pig manure, 70 parts of fruit/vegetable waste, fermentation 2
A month.
The organic fertilizer is made of following parts by weight raw material: pilose actinodaphne bark or leaf fallen leaves 50 parts, 50 parts of fruit/vegetable waste, lignin
45 parts, 20 parts of ferment bacterium, 9 parts of molasses fermented powder, 7 parts of Calusena lansium leaf extract, 7 parts of xanthium sibiricum, 20 parts of bone powder, natural pond
12 parts of gas pond residue.
The nutrient bag by coco bran, thin river sand, perlite, biomass carbon mixture as matrix.
It is described that using quick breeding by group culture method cultivation pilose actinodaphne bark or leaf tissue-cultured seedling, specific step is as follows:
(1) explant is handled: being chosen the lateral bud of staminiferous plant pilose actinodaphne bark or leaf, is cleaned up with clear water, the alcohol that suck dry moisture is put into 75% is molten
12s is impregnated in liquid, is impregnated 28min with aseptic water washing 4 times, then with 2% liquor natrii hypochloritis, with aseptic water washing 3 times, is used
Tweezers clamp cleaned lateral bud, on aseptic operating platform, blot surface moisture with the filter paper to sterilize.
(2) Primary culture: the explant after sterilizing being inoculated into the Primary culture base through high-temperature sterilization and is cultivated, and cultivates item
Part is 25 DEG C of temperature, intensity of illumination 2250lx, light application time 14h/d, and sterile culture is to sprouting in culturing room.
(3) squamous subculture: moving into the subculture medium through high-temperature sterilization for bud obtained by step (2), is 24 in cultivation temperature
DEG C, light intensity 2100lx, light application time cultivate 38d under conditions of being 15h/d.
(4) culture of rootage: aseptically, squamous subculture is obtained into no offspring, is inserted into root media and is given birth to
Root culture；Cultivation temperature is 27 DEG C, light intensity 21001x, light application time 14h/d.
The ingredient of Primary culture base are as follows: MS+ N6 benzyladenine 1.2mg/L+0 .6mg/L of methyl α-naphthyl acetate+vitamin B1
1 .0mg/L+molasses 3g/L+ agar 4g/L, culture medium pH value are 6.0；
The ingredient of subculture medium are as follows: MS+ N6 benzyladenine 1.8mg/L+ methyl α-naphthyl acetate 0.5mg/L+ vitamin B1 0.8mg/L
+ vitamin C 1.5mg/L+ glutamic acid 2.1mg/L+ molasses 3.5g/L+ agar 4g/L, culture medium pH value are 5.8；
The ingredient of root media are as follows: 1/2MS++methyl α-naphthyl acetate 0.2mg/L+ indolebutyric acid 0.8mg/L+ heteroauxin 0.2mg/L+
Molasses 4.5g/L+ agar 4g/L, culture medium pH value are 6.1.
Embodiment 2
A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf includes selection of land site preparation, cultivates seedling, transplanting, field management, harvesting, specifically
Content is as follows:
(1) selection of land site preparation: in the hillside fields in the south that Guangxi Daxin County selects to have abundant water resources, soil layer is deep, fertile as pilose actinodaphne bark or leaf
Planting site；It deep ploughs in October previous year of transplanting to soil, depth 45cm-55cm, application base manure, 450 kilograms/
Mu.
(2) it cultivates seedling: choosing the lateral bud of staminiferous plant pilose actinodaphne bark or leaf as explant, cultivated using quick breeding by group culture method fragrant
Bakelite tissue-cultured seedling grows to 5cm-8cm high to it, has 5-8 item root time shift to enter nutrient bag to be cultivated.
(3) it transplants: when seedling long supreme 0.8m-1.0m, hardening 10 days, then by its balled transplanting, transplanting seeding row spacing is
1.5m*2.0m, depth 35cm-45cm are put into seedling in kind of plant hole and irrigate root water after backfill, then are spreading one layer above
Fine earth；Sick seedling, dead seedling are removed after 15 days in time, and is filled the gaps with seedlings in time.
(4) field management:
1) after transplanting in 6 months, 2% aqueous solution of urea of spraying every month, urea mu dosage 40kg；After 6 months every other month
Organic fertilizer is applied, every is applied 1.5kg；After 1 year, 3 organic fertilizers are applied every year, and respectively in April, August part, December, mu is used
3 tons of amount；
2) as the long supreme 1.3m-1.5m of sapling, trunk is cut off, retains 3-5 main side branch, is evenly distributed on the surrounding of trunk；
The height for controlling pilose actinodaphne bark or leaf is no more than 2.5m；It also needs to trim sick branch, deadwood, overstocked branch, crossing branch every year, keeps logical
Wind light transmission reduces disease.
(5) it harvests: annual July and November, harvesting the leaf of pilose actinodaphne bark or leaf；Root is excavated, root skin is collected November,
Every pilose actinodaphne bark or leaf at least retains 3 complete main roots.
The base manure is made of following parts by weight raw material: 20 parts of cow dung, 50 parts of pig manure, 60 parts of fruit/vegetable waste, fermentation 1
A month.
The organic fertilizer is made of following parts by weight raw material: pilose actinodaphne bark or leaf fallen leaves 40 parts, 40 parts of fruit/vegetable waste, lignin
30 parts, 10 parts of ferment bacterium, 8 parts of molasses fermented powder, 6 parts of Calusena lansium leaf extract, 6 parts of xanthium sibiricum, 10 parts of bone powder, natural pond
10 parts of gas pond residue.
The nutrient bag is by coco bran, thin river sand mixture as matrix.
It is described that using quick breeding by group culture method cultivation pilose actinodaphne bark or leaf tissue-cultured seedling, specific step is as follows:
(1) explant is handled: being chosen the lateral bud of staminiferous plant pilose actinodaphne bark or leaf, is cleaned up with clear water, the alcohol that suck dry moisture is put into 75% is molten
15s is impregnated in liquid, is impregnated 25min with aseptic water washing 5 times, then with 2% liquor natrii hypochloritis, with aseptic water washing 2 times, is used
Tweezers clamp cleaned lateral bud, on aseptic operating platform, blot surface moisture with the filter paper to sterilize.
(2) Primary culture: the explant after sterilizing being inoculated into the Primary culture base through high-temperature sterilization and is cultivated, and cultivates item
Part is 24 DEG C of temperature, intensity of illumination 2500lx, light application time 12h/d, and sterile culture is to sprouting in culturing room.
(3) squamous subculture: moving into the subculture medium through high-temperature sterilization for bud obtained by step (2), is 24 in cultivation temperature
DEG C, light intensity 2200lx, light application time cultivate 40d under conditions of being 14h/d.
(4) culture of rootage: aseptically, squamous subculture is obtained into no offspring, is inserted into root media and is given birth to
Root culture；Cultivation temperature is 26 DEG C, light intensity 22001x, light application time 13h/d.
The ingredient of Primary culture base are as follows: MS+ N6 benzyladenine 1.2mg/L+0 .6mg/L of methyl α-naphthyl acetate+vitamin B1
1 .0mg/L+molasses 3g/L+ agar 4g/L, culture medium pH value are 6.0；
The ingredient of subculture medium are as follows: MS+ N6 benzyladenine 1.8mg/L+ methyl α-naphthyl acetate 0.5mg/L+ vitamin B1 0.8mg/L
+ vitamin C 1.5mg/L+ glutamic acid 2.1mg/L+ molasses 3.5g/L+ agar 4g/L, culture medium pH value are 5.8；
The ingredient of root media are as follows: 1/2MS++methyl α-naphthyl acetate 0.2mg/L+ indolebutyric acid 0.8mg/L+ heteroauxin 0.2mg/L+
Molasses 4.5g/L+ agar 4g/L, culture medium pH value are 6.1.
Embodiment 3
A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf includes selection of land site preparation, cultivates seedling, transplanting, field management, harvesting, specifically
Content is as follows:
(1) selection of land site preparation: in the hillside fields in the south that Guangxi Daxin County selects to have abundant water resources, soil layer is deep, fertile as pilose actinodaphne bark or leaf
Planting site；It deep ploughs in November previous year of transplanting to soil, depth 45cm-55cm, application base manure, 250 kilograms/
Mu.
(2) it cultivates seedling: choosing the lateral bud of staminiferous plant pilose actinodaphne bark or leaf as explant, cultivated using quick breeding by group culture method fragrant
Bakelite tissue-cultured seedling grows to 5cm-8cm high to it, has 5-8 item root time shift to enter nutrient bag to be cultivated.
(3) it transplants: when seedling long supreme 0.8m-1.0m, hardening 20 days, then by its balled transplanting, transplanting seeding row spacing is
1.5m*2.0m, depth 35cm-45cm are put into seedling in kind of plant hole and irrigate root water after backfill, then are spreading one layer above
Fine earth；Sick seedling, dead seedling are removed after 15 days in time, and is filled the gaps with seedlings in time.
(4) field management:
1) after transplanting in 6 months, 1% aqueous solution of urea of spraying every month, urea mu dosage 30kg；After 6 months every other month
Organic fertilizer is applied, every is applied 1.2kg；After 1 year, 3 organic fertilizers are applied every year, respectively in May, September part, January next year,
2 tons of dosage of mu；
2) as the long supreme 1.3m-1.5m of sapling, trunk is cut off, retains 3-5 main side branch, is evenly distributed on the surrounding of trunk；
The height for controlling pilose actinodaphne bark or leaf is no more than 2.5m；It also needs to trim sick branch, deadwood, overstocked branch, crossing branch every year, keeps logical
Wind light transmission reduces disease.
(5) it harvests: annual August part and December, harvesting the leaf of pilose actinodaphne bark or leaf；Root is excavated, root skin is collected December,
Every pilose actinodaphne bark or leaf at least retains 3 complete main roots.
The base manure is made of following parts by weight raw material: 50 parts of cow dung, 70 parts of pig manure, 80 parts of fruit/vegetable waste, fermentation 2
A month.
The organic fertilizer is made of following parts by weight raw material: pilose actinodaphne bark or leaf fallen leaves 60 parts, 60 parts of fruit/vegetable waste, lignin
60 parts, 30 parts of ferment bacterium, 10 parts of molasses fermented powder, 8 parts of Calusena lansium leaf extract, 8 parts of xanthium sibiricum, 30 parts of bone powder,
15 parts of methane tank residue.
The nutrient bag is by perlite, biomass carbon mixture as matrix.
It is described that using quick breeding by group culture method cultivation pilose actinodaphne bark or leaf tissue-cultured seedling, specific step is as follows:
(1) explant is handled: being chosen the lateral bud of staminiferous plant pilose actinodaphne bark or leaf, is cleaned up with clear water, the alcohol that suck dry moisture is put into 75% is molten
10s is impregnated in liquid, is impregnated 30min with aseptic water washing 3 times, then with 2% liquor natrii hypochloritis, with aseptic water washing 3 times, is used
Tweezers clamp cleaned lateral bud, on aseptic operating platform, blot surface moisture with the filter paper to sterilize.
(2) Primary culture: the explant after sterilizing being inoculated into the Primary culture base through high-temperature sterilization and is cultivated, and cultivates item
Part is 26 DEG C of temperature, intensity of illumination 2000lx, light application time 16h/d, and sterile culture is to sprouting in culturing room.
(3) squamous subculture: moving into the subculture medium through high-temperature sterilization for bud obtained by step (2), is 25 in cultivation temperature
DEG C, light intensity 2000lx, light application time cultivate 35d under conditions of being 16h/d.
(4) culture of rootage: aseptically, squamous subculture is obtained into no offspring, is inserted into root media and is given birth to
Root culture；Cultivation temperature is 26 DEG C, light intensity 20001x, light application time 15h/d.
The ingredient of Primary culture base are as follows: MS+ N6 benzyladenine 1.2mg/L+0 .6mg/L of methyl α-naphthyl acetate+vitamin B1
1 .0mg/L+molasses 3g/L+ agar 4g/L, culture medium pH value are 6.0；
The ingredient of subculture medium are as follows: MS+ N6 benzyladenine 1.8mg/L+ methyl α-naphthyl acetate 0.5mg/L+ vitamin B1 0.8mg/L
+ vitamin C 1.5mg/L+ glutamic acid 2.1mg/L+ molasses 3.5g/L+ agar 4g/L, culture medium pH value are 5.8；
The ingredient of root media are as follows: 1/2MS++methyl α-naphthyl acetate 0.2mg/L+ indolebutyric acid 0.8mg/L+ heteroauxin 0.2mg/L+
Molasses 4.5g/L+ agar 4g/L, culture medium pH value are 6.1.
For the drug effect of pilose actinodaphne bark or leaf for examining the present invention to plant out, pilose actinodaphne bark or leaf that the present invention is planted out by applicant and
Wild pilose actinodaphne bark or leaf sends to inspection center together and carries out constituent analysis, is found contained by wild pilose actinodaphne bark or leaf by constituent analysis comparison
Medicinal ingredient, the pilose actinodaphne bark or leaf that the present invention obtains similarly contains, and content is not much different.
Claims (5)
1. a kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf, which is characterized in that the method includes selection of land site preparation, cultivate seedling,
Transplanting, field management, harvesting, particular content are as follows:
(1) it selection of land site preparation: selects to have abundant water resources, the planting site of soil layer is deep, fertile hillside fields in the south as pilose actinodaphne bark or leaf；It is transplanting
Deep plough to soil in month previous year 10-11, depth 45cm-55cm, apply base manure, 250-450 kgs/acre；
(2) it cultivates seedling: choosing the lateral bud of staminiferous plant pilose actinodaphne bark or leaf as explant, pilose actinodaphne bark or leaf is cultivated using quick breeding by group culture method
Tissue-cultured seedling grows to 5cm-8cm high to it, has 5-8 item root time shift to enter nutrient bag to be cultivated；
(3) it transplants: when seedling long supreme 0.8m -1.0m, hardening 10-20 days, then by its balled transplanting, transplanting seeding row spacing is
1.5m*2.0m, depth 35cm-45cm are put into seedling in kind of plant hole and irrigate root water after backfill, then are spreading one layer above
Fine earth；Sick seedling, dead seedling are removed after 15 days in time, and is filled the gaps with seedlings in time；
(4) field management:
1) after transplanting in 6 months, 1%-2% aqueous solution of urea of spraying every month, urea mu dosage 30-40kg；After 6 months every
One month application organic fertilizer, every is applied 1.2-1.5kg；After 1 year, 3 organic fertilizers are applied every year, respectively in May in April-, 8
Month-September part, January next year in December-, 2-3 tons of mu dosage；
2) as the long supreme 1.3m-1.5m of sapling, trunk is cut off, retains 3-5 main side branch, is evenly distributed on the surrounding of trunk；
The height for controlling pilose actinodaphne bark or leaf is no more than 2.5m；It also needs to trim sick branch, deadwood, overstocked branch, crossing branch every year, keeps logical
Wind light transmission reduces disease；
(5) it harvests: annual 7-8 month and 11-12 month, harvesting the leaf of pilose actinodaphne bark or leaf；11-12 month root is excavated, root is collected
Skin, every pilose actinodaphne bark or leaf at least retain 3 complete main roots.
2. the artificial method for planting of medicinal pilose actinodaphne bark or leaf according to claim 1, which is characterized in that the base manure is by following weight
Number raw material composition: 20-50 parts of cow dung, 50-70 parts of pig manure, 60-80 parts of fruit/vegetable waste, fermentation 1-2 months.
3. the artificial method for planting of medicinal pilose actinodaphne bark or leaf according to claim 1, which is characterized in that the organic fertilizer is by following heavy
Measure number raw material composition: pilose actinodaphne bark or leaf fallen leaves 40-60 parts, 40-60 parts of fruit/vegetable waste, 30-60 parts of lignin, ferment bacterium 10-30
Part, powder 8-10 parts molasses fermented, 6-8 parts of Calusena lansium leaf extract, 6-8 parts of xanthium sibiricum, 10-30 parts of bone powder, biogas
Residue 10-15 parts of pond.
4. the artificial method for planting of medicinal pilose actinodaphne bark or leaf according to claim 1, which is characterized in that the nutrient bag by coco bran,
Thin river sand, perlite, in biomass carbon two or more mixture as matrix.
5. the artificial method for planting of medicinal pilose actinodaphne bark or leaf according to claim 1, which is characterized in that described to use tissue culture quick propagation
Growing method cultivation pilose actinodaphne bark or leaf tissue-cultured seedling, specific step is as follows:
(1) explant is handled: being chosen the lateral bud of staminiferous plant pilose actinodaphne bark or leaf, is cleaned up with clear water, the alcohol that suck dry moisture is put into 75% is molten
10-15s is impregnated in liquid, is impregnated 25-30min with aseptic water washing 3-5 times, then with 2% liquor natrii hypochloritis, is rushed with sterile water
It washes 2-3 times, cleaned lateral bud is clamped with tweezers, on aseptic operating platform, blot surface moisture with the filter paper to sterilize；
(2) Primary culture: the explant after sterilizing is inoculated into the Primary culture base through high-temperature sterilization and is cultivated, condition of culture is
24-26 DEG C of temperature, intensity of illumination 2000-2500lx, light application time 12-16h/d, sterile culture is to sprouting in culturing room；
(3) squamous subculture: moving into the subculture medium through high-temperature sterilization for bud obtained by step (2), is 24-25 in cultivation temperature
DEG C, light intensity 2000-2200lx, light application time cultivate 35-40d under conditions of being 14-16h/d；
(4) culture of rootage: aseptically, squamous subculture is obtained into no offspring, is inserted into root media and carries out training of taking root
It supports；Cultivation temperature is 26-28 DEG C, light intensity 2000-22001x, light application time 13-15h/d；
The ingredient of Primary culture base are as follows: MS+ N6 benzyladenine 1.2mg/L+0 .6mg/L of methyl α-naphthyl acetate+vitamin B1 1
.0mg/L+molasses 3g/L+ agar 4g/L, culture medium pH value are 6.0；
The ingredient of subculture medium are as follows: MS+ N6 benzyladenine 1.8mg/L+ methyl α-naphthyl acetate 0.5mg/L+ vitamin B1 0.8mg/L+
Vitamin C 1.5mg/L+ glutamic acid 2.1mg/L+ molasses 3.5g/L+ agar 4g/L, culture medium pH value are 5.8；
The ingredient of root media are as follows: 1/2MS++methyl α-naphthyl acetate 0.2mg/L+ indolebutyric acid 0.8mg/L+ heteroauxin 0.2mg/L+
Molasses 4.5g/L+ agar 4g/L, culture medium pH value are 6.1.
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| CN110558141A (en) * | 2019-10-15 | 2019-12-13 | 韦国安 | planting method of cherokee rose fruit |
| KR102555625B1 (en) * | 2023-01-30 | 2023-07-18 | 디와이엠 주식회사 | Chinese hackberry planting method |
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