CN103141260B - Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest - Google Patents

Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest Download PDF

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CN103141260B
CN103141260B CN201310086895.0A CN201310086895A CN103141260B CN 103141260 B CN103141260 B CN 103141260B CN 201310086895 A CN201310086895 A CN 201310086895A CN 103141260 B CN103141260 B CN 103141260B
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bamboo
culture
chloranthus glaber
days
mao
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CN201310086895.0A
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CN103141260A (en
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朱淑颖
姜叶琴
张海洋
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湖州师范学院
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Abstract

The invention relates to a method for ecologically interplanting sarcandra glabra under a phyllostachys edulis forest. The method comprises the steps of: (1) tissue culture and seedling raising: sarcandra glabra blades are selected as explants to conduct adventitious bud induction, an adventitious bud induction culture medium uses an MS (Murashige and Skoog) culture medium as a basic culture medium and additionally comprises 2.0 to 4.0mg/L of TDZ (thidiazuron), 0.1 to 0.2mg/L of NAA (naphthyl acetic acid), 100 to 150g/L of banana puree and 0.5 to 1.0g/L of activated carbon, and then adventitious buds are subjected to enrichment culture and rooting culture; (2) seedling hardening: bottom seedlings are conveyed to under the phyllostachys edulis forest to be hardened; (3) transplantation: tissue culture seedlings are transplanted to a north slope bamboo forest with a number of living stems (NLS) of phyllostachys edulis of 150 to 400 plants/mu or a south slope bamboo forest with an NLS of 200 to 400 plants/mu; (4) forest management: the second intertillage is conducted in June and September every year; and (5) harvest: the sarcandra glabra is harvested in November to December every year. By adopting the method, the culture period of the sarcandra glabra can be shortened and the yield of the sarcandra glabra can be increased.

Description

A kind of method of ecological interplanting Chloranthus glaber under mao bamboo woods
Technical field
The invention belongs to agricultural planting technique field, be specially the method for ecological interplanting Chloranthus glaber under a kind of mao bamboo woods.
Background technology
Chloranthus glaber (Sarcandra glabra (Thunb.) Nakai), another name: Glabrous Sarcandra Herb, synthetism lotus, sarcandra, diffuse dayflower herb etc. are Chloranthaceae Sarcandra perennial evergreen fruticuli.Complete stool hyoscine, have anti-inflammation, clearing heat and detoxicating, dispel rheumatism, promoting blood circulation and stopping pain, the effects such as synthetism of stimulating the menstrual flow, be used for the treatment of various diseases associated with inflammation, rheumatic arthritis, sore swollen toxin, traumatic injury, fracture etc., remarkable to Several Kinds of Malignancy effect, be the good traditional Chinese medicine that a kind of pharmacological action is extensive, toxicity is low.Along with take the continually developing of new product that Chloranthus glaber is raw material, increasing to its demand, traditional complete stool mode of excavating causes going to wreck property of wild resource to be destroyed, and cause raw medicinal material in short supply, so Chloranthus glaber has boundless plantation DEVELOPMENT PROSPECT.Under natural conditions, Chloranthus glaber is born in hillside, the dark and damp place of cheuch sylvan life, 420~1500 meters of height above sea level.The environment of happiness warm moist, avoids high light direct projection and long high-temperature drying.The habit of growth of Chloranthus glaber uniqueness requires planting process that certain condition of sheltering from heat or light must be provided, and the measure of manually sheltering from heat or light will improve planting cost greatly.If Chloranthus glaber is interplanted in mao bamboo woods, can effectively address this problem.
Mao bamboon is distributed in 23 ° 23 ' ~ 32 ° 20 ' of China's north latitude, in the broad regions that east longitude is 104 ° 30 ' ~ 122 °, is that China distributes the most extensively, area is maximum, one of economic, ecological, social benefit is best bamboo kind.There are mao bamboon cultivation in/3rd above provinces and regions, the whole nation, municipality directly under the Central Government at present, and existing mao bamboo woods area is 2,800,000 hm nearly 2, wherein major part is the material mao bamboo woods that Management Technology is simple and input-output ratio is high.And the continuous expansion of the downslide of bamboo shoots price in recent years and bamboo wood range of application makes material further increase by the cultivated area of woods.But single plantation mao bamboon economic benefit is limited, and in mao bamboo woods, interplant Chloranthus glaber, not only save the link of shading in Chloranthus glaber cultivation, reduced planting cost, the special odor and the efficacy of a drug that also because of Chloranthus glaber, have can reduce damage by disease and insect, the more important thing is conditions such as can making full use of soil and light, temperature, water, fertilizer, the output of raising the multiple-cropping index and obviously increase economic benefit.
In addition, interplant on a large scale Chloranthus glaber and must first solve seedling breeding problem, its traditional modes of reproduction has cuttage, sowing and plant division etc., but reproduction coefficient is low, survival rate is low, seedling cost is relatively high and be subject to weather influence.Utilizing Plant Tissue Breeding means to set up quick reproduction technique system is optimal selection.Publication number be CN 101194593A Introduction To Cn Patent a kind of tissue culture and rapid propagation method of Chloranthus glaber, the method be take the root system of aseptic seedling and is obtained regrowth as explant passes through callus approach, but it is examination material that the method must be take the test-tube plantlet that the induction of Chloranthus glaber aseptic seeding or stem segment with axillary buds obtains, complex steps, production cycle are long, and still face Chloranthus glaber seed maturity rate and utilization percent is low, seed source is difficult, drawing materials is subject to seasonal restrictions the problem low with germination rate.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, the method for ecological interplanting Chloranthus glaber under a kind of mao bamboo woods is provided, to reduce the output of the planting cost of Chloranthus glaber, the cultivation period that shortens Chloranthus glaber, raising Chloranthus glaber.
The scheme that the present invention solve the technical problem is: a kind of method of ecological interplanting Chloranthus glaber under mao bamboo woods, is characterized in that: comprise the steps,
(1) tissue culture
Choose Chloranthus glaber blade and make explant, after sterilization, blade is cut into small pieces and is placed on adventitious bud induction culture base, inoculate after 5 days and transfer in fresh adventitious bud induction culture base and continue to cultivate 30~35 days, adventitious bud induction culture base is to take MS as minimal medium, additional 2.0~4.0 mg/L TDZ, 0.1~0.2 mg/L NAA, 100~150g/L banana puree and 0.5~1.0g/L active carbon;
The indefinite bud that blade edge is grown is cut into single bud, is inoculated in proliferated culture medium, and proliferated culture medium is to take MS as minimal medium, additional 1.0~2.0 mg/L TDZ and 0.05~0.1 mg/L NAA;
Cut the simple bud of high 2~3cm, be inoculated in root media, root media is to take 1/2MS as minimal medium, additional 0.1~0.2 mg/L NAA;
All medium all add 6.0 mg/L agar and 30.0 mg/L sucrose above, and group training chamber condition of culture is 25 ± 2 ℃ of temperature, illuminance 1500~2000 lx, illumination every day 12 h;
(2) hardening
In late April, by culture of rootage, the bottle seedling about 20 days is transported to mao bamboo woods lower refining seedling, does not open bottle cap hardening 10~12 days, then opens bottle cap hardening 2~3 days;
(3) transplant
After hardening is completed, group training transplantation of seedlings is the southern slope bamboo grove that the north slope bamboo grove of 150~400 plants/acre or vertical bamboo degree are 200~400 plants/acre in the vertical bamboo degree of mao bamboon;
(4) forest management
Use and urge whip fertilizer to intertill for the first time annual June in conjunction with bamboo grove, dark 10~15cm, and apply urea or the composite fertilizer of 5kg/ mu, intertill September for the second time; Field planting then winter at root ground mulching straw around;
(5) gather
Field planting Second Year plays the Chloranthus glaber of gathering annual 11~December, and completes and gather before cutting down bamboo.
As further technical scheme, in described transplant step, Chloranthus glaber seedling is longitudinally in a row planted along hillside physical features, spacing in the rows 35~40cm, two row's spacing 25~30cm, arrange a pavement that 1.0~1.2m is wide every 6~8 Lysimachia sikokiana corals.
As technical scheme further, after described group of training transplantation of seedlings at its foliage-spray 0.1% calcium chloride.
As technical scheme further, the type of described bamboo grove is material mao bamboo woods.
Beneficial effect of the present invention has:
(1) at mao bamboon forest interplanting Chloranthus glaber, need not occupy cultivated land separately, not need prior afforestation, improve land utilization rate, reduce the planting cost of Chloranthus glaber; Mao bamboo woods are the Chloranthus glaber moisturizing of sheltering from heat or light, thereby make Chloranthus glaber not well grown by direct sunlight, and the special odor that Chloranthus glaber has and the efficacy of a drug can reduce the damage by disease and insect of mao bamboo woods again, make two kinds of crop harmonic growths, form benign cycle; On the other hand, in the time of farming Chloranthus glaber, bamboo grove is also fostered, be conducive to the management of mao bamboo woods, changed the traditional single cropping pattern of mao bamboo woods, can greatly improve farmers' income;
(2) the present invention makes explant with Chloranthus glaber blade, set up a kind of Chloranthus glaber blade high-efficiency regeneration system, by the method carry out that seedling breeding is simple and easy to do, efficient stable, material source is abundant and endophytic bacterial contamination is few, can obtain at short notice the seedling that a large amount of germplasms are good, proterties is unified, for extensive, standardization interplanting Chloranthus glaber provide powerful guarantee;
(3) by hardening off method hardening of the present invention, and in conjunction with suitable transplanting method, Chloranthus glaber group training seedling can directly be transplanted to mao bamboo woods, does not need, through the nursery phase, to have saved the time, has also saved the cost that construction of nursery garden, management and secondary are transplanted.
(4) the present invention is along the longitudinal in a row plantation Chloranthus glaber of hillside physical features, and every 6~8 rows, a pavement is set, and vacant lot is provided not only to forest management, operation, also convenient felling mao bamboon operation mao bamboon is pulled.
(5) bamboo shoot are not suitable for interplanting Chloranthus glaber with woods or the dual-purpose woods of bamboo shoot material, because it need to dig in a large number bamboo shoot and cultivate every year again easy damaged sylvan life crop during farming.The type of bamboo grove of the present invention is material mao bamboo woods, can reduce to greatest extent the impact of personnel's activity on Chloranthus glaber.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and test is carried out under the mao bamboo woods of Mountainous Region, Zhejiang, has selected 4 different material mao bamboo woods, tests simultaneously.
embodiment 1:
(1) tissue culture
Choose the disease-free tender leaf of health of Chloranthus glaber and make explant, first use 75% alcohol-pickled 20s in superclean bench, then soak 1min with 0.1% mercuric chloride solution, aseptic water washing 3~4 times, is cut into the about 1cm of area 2the blockage of left and right, standby.Blade by after sterilization, lies against in adventitious bud induction culture base upward with adaxial and its surface, inoculates after 5 days and transfers in fresh culture and continue to cultivate 30 days.Adventitious bud induction culture base is to take MS as minimal medium, additional 2.5 mg/L TDZ, 0.15 mg/L NAA, 120g/L banana puree and 0.8g/L active carbon; Blade inoculation is to fresh culture after 15 days, and incision is slightly expanded and had green bud point to occur, starts subsequently to have the regeneration of indefinite bud.While cultivating 30 days, explant regeneration frequency and average regenerated adventitious bud number are respectively 82.1% and 5.3.The indefinite bud that blade edge is grown is cut into single bud, is inoculated in proliferated culture medium, and proliferated culture medium is to take MS as minimal medium, additional 1.2mg/L TDZ and 0.06 mg/L NAA.Available identical proliferated culture medium is shoot proliferation repeatedly, and once, the average propagation multiple of Multiple Buds is 6.2 to every 25~30 days subcultures.Cut the simple bud of high 2~3cm, be inoculated in root media, root media is to take 1/2MS as minimal medium, additional 0.15 mg/L NAA.About 12 days, start to take root, while being cultured to 20 days, rooting rate 100%.The time that starts culture of rootage determines according to transplanting time.All medium all add 6.0 mg/L agar and 30.0 mg/L sucrose above, and group training chamber condition of culture is 25 ± 2 ℃ of temperature, illuminance 1500~2000 lx, illumination every day 12 h.
(2) hardening
In late April, 2009, the bottle seedling by culture of rootage about 20 days is transported under traffic convenience, water source is convenient, physical features is smooth mao bamboo woods and concentrates hardening.Bottle seedling is neatly put on ground by bottle spacing 5cm * 5cm, first do not opened bottle cap hardening 10 days, then open bottle cap hardening 3 days, make group training seedling progressively adapt to plantation environment.After hardening completes, group training seedling presss from both sides out with tweezers, and water washes away after root medium minute to be delivered to periphery forest land and transplant.
(3) transplant
Start whole ground the first tenday period of a month in May to the middle ten days and transplant in the material mao bamboo woods that are positioned at north slope, the vertical bamboo degree of north slope bamboo grove mao bamboon is controlled to 150~400 plants/acre, total cultivated area is 2.5 mu.Surgery weeds, shrub in woods, dig up the bamboo root and stem of certain plants, shrub is first-class, then plough, flatten.Chloranthus glaber seedling is in a row planted along hillside physical features is longitudinal, spacing in the rows 35~40cm, two row's spacing 25~30cm, after every plantation 6~8 Lysimachia sikokiana corals, the reserved wide pavement of 1.0~1.2m, so that cut down mao bamboon operation and mao bamboon pulled; During plantation, can distribute and slightly adjust according to bamboo, needn't too impose rule standardized.During transplanting, dig cave, the dark 10~15cm in cave, the table soil of humus is rich in backfill on a small quantity, but cave deeply still should not be less than 5cm, and plant is put into cave, covers root system, compacting with table soil as far as possible.Irrigate normal root water, foliage-spray transpiration inhibitor 0.1% calcium chloride, spray once, connects and sprays 3 times week about.In case of weather arid need be watered in time.Transplant and can water for the first time the night soil becoming thoroughly decomposed in latter about one month, the dilution ratio of night soil and water is 1: 15, uses night soil later in good time.In the time of one month, adding up transplanting survival rate is 93%.
(4) forest management
Use and urge whip fertilizer to intertill for the first time annual June in conjunction with bamboo grove, dark 10~15cm, and apply the urea of 5kg/ mu, and intertill September for the second time, weeds a garden, and keeps loosing soil, and operating process takes into account mao bamboon and fosters; Keep ground moistening vegetative period, in field planting summer then, as the not rainfall over 2 weeks, need to water in time.In December, 2009, at root ground mulching straw around, both can heat-insulating and cold, can promote again the beginning of spring plant early raw fast long.From root 20cm place trench digging, be buried in the earth straw as fertilizer sources in March, 2010.
(5) gather
The Chloranthus glaber of gathering in December, 2010, in December, 2011 and in November, 2012, selects fine day to gather, and extracts cauline leaf on the ground, cut stay greatly little, allow branch tenderer, length≤20cm leave continued growth, and long, older, ripe stem branch is being cut off apart from basal part of stem 5~10cm place.Play Chloranthus glaber growth November and stop gradually, blade is dark green, fruit reddens, now biomass reach the highest, harvesting optimum; And harvest Chloranthus glaber, cut down again mao bamboon, not only meet the management rule of bamboo grove but also make felling process convenient.Can gather in once every year later.The disposable plantation of Chloranthus glaber, income for many years, circulation results reach 20 years.
The medicinal material of harvesting in 3 years is all sent to city medicine inspecting institute and detects, and Isofraxidin content is 0.031~0.035% after testing, reaches standards of pharmacopoeia (must not be less than 0.020%) completely.Within 3 years, obtain respectively 525,852 and 956 kilograms of Chloranthus glaber dry products, amount to into yield per unit area and be respectively 210,340.8 and 382.4 thousand grams/acre, cumulative year after year.
From between 2009 to 2012 4 years, there is not any damage by disease and insect in mao bamboon, and the average diameter of a cross-section of a tree trunk 1.3 meters above the ground of newborn mao bamboon has increased 10% left and right.
embodiment 2:
(1) tissue culture
Choose the disease-free tender leaf of health of Chloranthus glaber and make explant, first use 75% alcohol-pickled 20s in superclean bench, then soak 1min with 0.1% mercuric chloride solution, aseptic water washing 3~4 times, is cut into the about 1cm of area 2the blockage of left and right, standby.Blade by after sterilization, lies against in adventitious bud induction culture base upward with adaxial and its surface, inoculates after 5 days and transfers in fresh culture and continue to cultivate 35 days.Adventitious bud induction culture base is to take MS as minimal medium, additional 2.0 mg/L TDZ, 0.1 mg/L NAA, 150g/L banana puree and 0.5g/L active carbon; Blade inoculation is to fresh culture after 17 days, and incision is slightly expanded and had green bud point to occur, starts subsequently to have the regeneration of indefinite bud.While cultivating 30 days, explant regeneration frequency and average regenerated adventitious bud number are respectively 80.2% and 4.9.The indefinite bud that blade edge is grown is cut into single bud, is inoculated in proliferated culture medium, and proliferated culture medium is to take MS as minimal medium, additional 1.0mg/L TDZ and 0.1 mg/L NAA.Available identical proliferated culture medium is shoot proliferation repeatedly, and once, the average propagation multiple of Multiple Buds is 5.7 to every 25~30 days subcultures.Cut the simple bud of high 2~3cm, be inoculated in root media, root media is to take 1/2MS as minimal medium, additional 0.2 mg/L NAA.About 14 days, start to take root, while being cultured to 20 days, rooting rate 100%.The time that starts culture of rootage determines according to transplanting time.All medium all add 6.0 mg/L agar and 30.0 mg/L sucrose above, and group training chamber condition of culture is 25 ± 2 ℃ of temperature, illuminance 1500~2000 lx, illumination every day 12 h.
(2) hardening
In late April, 2009, the bottle seedling by culture of rootage about 20 days is transported under traffic convenience, water source is convenient, physical features is smooth mao bamboo woods and concentrates hardening.Bottle seedling is neatly put on ground by bottle spacing 5cm * 5cm, first do not opened bottle cap hardening 12 days, then open bottle cap hardening 2 days, make group training seedling progressively adapt to plantation environment.After hardening completes, group training seedling presss from both sides out with tweezers, and water washes away after root medium minute to be delivered to periphery forest land and transplant.
(3) transplant
Start whole ground the first tenday period of a month in May to the middle ten days and transplant in the material mao bamboo woods that are positioned at Nan Po, the vertical bamboo degree of southern slope bamboo grove mao bamboon is controlled to 200~400 plants/acre, total cultivated area is also 2.5 mu.Surgery weeds, shrub in woods, dig up the bamboo root and stem of certain plants, shrub is first-class, then plough, flatten.Chloranthus glaber seedling is in a row planted along hillside physical features is longitudinal, spacing in the rows 35~40cm, two row's spacing 25~30cm, after every plantation 6~8 Lysimachia sikokiana corals, the reserved wide pavement of 1.0~1.2m, so that cut down mao bamboon operation and mao bamboon pulled; During plantation, can distribute and slightly adjust according to bamboo, needn't too impose rule standardized.During transplanting, dig cave, the dark 10~15cm in cave, the table soil of humus is rich in backfill on a small quantity, but cave deeply still should not be less than 5cm, and plant is put into cave, covers root system, compacting with table soil as far as possible.Irrigate normal root water, foliage-spray transpiration inhibitor 0.1% calcium chloride, spray once, connects and sprays 3 times week about.In case of weather arid need be watered in time.Transplant and can water for the first time the night soil becoming thoroughly decomposed in latter about one month, the dilution ratio of night soil and water is 1: 20, uses night soil later in good time.In the time of one month, adding up transplanting survival rate is 92%.
(4) forest management
Use and urge whip fertilizer to intertill for the first time annual June in conjunction with bamboo grove, dark 10~15cm, and apply the composite fertilizer of 5kg/ mu, and intertill September for the second time, weeds a garden, and keeps loosing soil, and operating process takes into account mao bamboon and fosters; Keep ground moistening vegetative period, in field planting summer then, as the not rainfall over 2 weeks, need to water in time.In December, 2009, at root ground mulching straw around, both can heat-insulating and cold, can promote again the beginning of spring plant early raw fast long.From root 20cm place trench digging, be buried in the earth straw as fertilizer sources in March, 2010.
(5) gather
The Chloranthus glaber of gathering in December, 2010, in December, 2011 and in November, 2012, gathering to be operated in before cutting down bamboo completes, and results situation and embodiment 1 are without significant difference.
embodiment 3:
The operating procedure of the present embodiment and parameter are with reference to embodiment 1, different: blade adventitious bud induction culture base is to take MS as minimal medium, additional 4.0 mg/L TDZ, 0.2 mg/L NAA, 100g/L banana puree and 1.0g/L active carbon; Blade inoculation is to fresh culture after 15 days, and incision is slightly expanded and had green bud point to occur, starts subsequently to have the regeneration of indefinite bud.While cultivating 30 days, explant regeneration frequency and average regenerated adventitious bud number are respectively 78.4% and 4.7.The indefinite bud that blade edge is grown is cut into single bud, is inoculated in proliferated culture medium, and proliferated culture medium is to take MS as minimal medium, additional 2.0mg/L TDZ and 0.05 mg/L NAA.Available identical proliferated culture medium is shoot proliferation repeatedly, and once, the average propagation multiple of Multiple Buds is 6.0 to every 25~30 days subcultures.Cut the simple bud of high 2~3cm, be inoculated in root media, root media is to take 1/2MS as minimal medium, additional 0.1mg/L NAA.About 14 days, start to take root, while being cultured to 20 days, rooting rate 100%.After group training seedling upgrowth situation, transplanting, survival rate and results situation and embodiment 1 are without significant difference.
embodiment 4:
The operating procedure of the present embodiment and parameter are with reference to embodiment 2, different: during transplanting, select 200 plants/acre of following southern slope bamboo groves of vertical bamboo degree.Plant after 1 month, transplanting survival rate is only 55% left and right.In process of growth, because solar radiation has the dried-up phenomenon in blade face, severe patient complete stool is dead.In the autumn of plantation Second Year, Chloranthus glaber survival rate is 20% left and right, and plant is short and small, leaf color is lighter, partially yellow, gather in.

Claims (4)

1. a method for ecological interplanting Chloranthus glaber under mao bamboo woods, is characterized in that: comprises the steps,
(1) tissue culture
Choose Chloranthus glaber blade and make explant, after sterilization, blade is cut into small pieces and is placed on adventitious bud induction culture base, inoculate after 5 days and transfer in fresh adventitious bud induction culture base and continue to cultivate 30~35 days, adventitious bud induction culture base is to take MS as minimal medium, additional 2.0~4.0 mg/L TDZ, 0.1~0.2 mg/L NAA, 100~150g/L banana puree and 0.5~1.0g/L active carbon;
The indefinite bud that blade edge is grown is cut into single bud, is inoculated in proliferated culture medium, and proliferated culture medium is to take MS as minimal medium, additional 1.0~2.0 mg/L TDZ and 0.05~0.1 mg/L NAA;
Cut the simple bud of high 2~3cm, be inoculated in root media, root media is to take 1/2MS as minimal medium, additional 0.1~0.2 mg/L NAA;
All medium all add 6.0 mg/L agar and 30.0 mg/L sucrose above, and group training chamber condition of culture is 25 ± 2 ℃ of temperature, illuminance 1500~2000 lx, illumination every day 12 h;
(2) hardening
In late April, by culture of rootage, the bottle seedling about 20 days is transported to mao bamboo woods lower refining seedling, does not open bottle cap hardening 10~12 days, then opens bottle cap hardening 2~3 days;
(3) transplant
After hardening is completed, group training transplantation of seedlings is the southern slope bamboo grove that the north slope bamboo grove of 150~400 plants/acre or vertical bamboo degree are 200~400 plants/acre in the vertical bamboo degree of mao bamboon;
(4) forest management
Use and urge whip fertilizer to intertill for the first time annual June in conjunction with bamboo grove, dark 10~15cm, and apply urea or the composite fertilizer of 5kg/ mu, intertill September for the second time; Field planting then winter at root ground mulching straw around;
(5) gather
Field planting Second Year plays the Chloranthus glaber of gathering annual 11~December, and completes and gather before cutting down bamboo.
2. the method for ecological interplanting Chloranthus glaber under a kind of mao bamboo woods as claimed in claim 1, it is characterized in that: in described transplant step, Chloranthus glaber seedling is longitudinally in a row planted along hillside physical features, spacing in the rows 35~40cm, two row's spacing 25~30cm, arrange a pavement that 1.0~1.2m is wide every 6~8 Lysimachia sikokiana corals.
3. the method for ecological interplanting Chloranthus glaber under a kind of mao bamboo woods as claimed in claim 1, is characterized in that: after described group of training transplantation of seedlings at its foliage-spray 0.1% calcium chloride.
4. the method for ecological interplanting Chloranthus glaber under a kind of mao bamboo woods as claimed in claim 1, is characterized in that: the type of described bamboo grove is material mao bamboo woods.
CN201310086895.0A 2013-03-18 2013-03-18 Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest CN103141260B (en)

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