CN103875672A - Walnut early frost anti-freezing agent - Google Patents
Walnut early frost anti-freezing agent Download PDFInfo
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- CN103875672A CN103875672A CN201310708599.XA CN201310708599A CN103875672A CN 103875672 A CN103875672 A CN 103875672A CN 201310708599 A CN201310708599 A CN 201310708599A CN 103875672 A CN103875672 A CN 103875672A
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Abstract
The invention discloses a walnut early frost anti-freezing agent which is characterized by being formed by compounding an ethanol solution of abscisic acid, an acetic acid solution of insect chitosan, an aqueous solution of proline, an aqueous solution of trehalose, ethanol, glycerol and water. With the novel plant anti-freezing agent, walnut endogenous anti-freezing metabolism is induced, leaf falling is advanced for avoiding early frost damage, and thus a walnut early frost prevention effect is achieved. According to the anti-freezing agent, leaves of walnut trees can be promoted to fall within about 15 days in advance under the same temperature condition, and thus the early frost damage is effectively avoided; the endogenous anti-freezing metabolism is performed in the induction period, and thus the early frost prevention effect of walnut trees is achieved.
Description
Technical field
The invention belongs to plant growth regulator field, relate to a kind of walnut early frost antifreezing agent.Its principle is to promote in advance walnut to fall leaves, enter resting stage.
Background technology
Walnut is China's mountain development economy and the mainstay industry of increasing farmers' income, one of main dry fruit export varieties of Ye Shi China.Along with the fast development of rural economy and improving constantly of people's living standard, the nutritive value of walnut and health care are favored by consumers in general.Frost damage is the key factor of restriction China's walnut natural production and harvest, and it affects much larger than walnut damage by disease and insect, in provinces and regions such as Yunnan, China's walnut main producing region, Shaanxi, Xinjiang, Shanxi, Hebei, all has walnut frost damage problem.Walnut suffer frost generally in the fall early frost or spring cold in the late spring occur period, walnut frost damage is produced and is had very serious implications walnut, not only cause branch slightly dehydration drain death, flower, leaf, bud are withered to come off, and can cause the significantly underproduction of walnut, even has no harvest.The anti-control techniques of research walnut frost damage, the prevention of carrying out walnut frost damage is of great significance the Walnut tool of China.
Summary of the invention
The object of the invention is a kind of walnut early frost antifreezing agent, change traditional plant antifreezing agent generally uses the deficiency of plant hormone.The present invention utilizes nutritive element and plant endogenous property semiochemicals to regulate walnut to grow, and reaches to delay bud and leaf bud sprouts, promotes the effect of leaf bud, the growth of bud later stage to avoid the frostbite of early frost to the sprout that sprouts, to reach the effect of anti-early frost.
Technical scheme of the present invention is: a kind of walnut early frost antifreezing agent, is characterized in that: this walnut early frost antifreezing agent be by the acetum of the ethanolic solution of abscisic acid, insect shell glycan, the proline aqueous solution, aqueous trehalose solution, ethanol, G & W is composite forms.
The walnut early frost antifreezing agent of preparation 1L needs composite the forming of raw material of following mass volume ratio: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0 % of insect shell glycan, proline aqueous solution 0.01-1.0 %, aqueous trehalose solution 0.01-1.0 %, ethanol 5-30 %, glycerine 10-50 %, surplus are water.
The preparation method of described walnut early frost antifreezing agent is: successively the ethanolic solution of the abscisic acid of formula ratio, insect chitosan solution, the proline aqueous solution, aqueous trehalose solution are mixed, and then add successively ethanol, the G & W of formula ratio, fully mix bottling.
Described walnut early frost antifreezing agent sprays for 2 weeks before early spring, walnut bud was sprouted.
Before described walnut early frost antifreezing agent uncork, be soaked in 50-60 ℃ of hot water 5 minutes, take out firmly concussion and shaking up, guaranteeing do not have solid to separate out in bottle; When use, with 500 times of running water dilutions.
Advantage of the present invention is: antifreezing agent of the present invention has the walnut bud of delay, leaf bud is sprouted effect, postpones bud, leaf bud sprout time reaches 10～15 days, can reach the effect of avoiding early frost harm, thereby reaches anti-early frost, antifreeze effect.Meanwhile, the trace element in formula and shitosan also can further excite other degeneration-resistant reaction of plant and promote the late growing stage of bud, leaf bud, and the growth of walnut body is not had a negative impact.Compare with traditional plant antifreezing agent, this prescription is containing any plant hormone class growth regulator, character gentleness, growth and development of plants do not caused to interference, belongs to trophism regulating plant resistance and retarded germination effect.Wherein, abscisic acid (ABA) is ubiquitous natural materials in plant, and equal natural containing in the edible fruit of the mankind, vegetables, grain, to the mankind and Environmental security.Abscisic acid is a kind of plant hormone with sequiterpene structure, and abscisic acid can promote the accumulation of the reserve substance of seed, fruit, particularly storage protein and sugar part; Inducing plant resistance is played to vital effect.Shitosan has obvious physiological regulation function to plant ammonia assimilation key enzyme, can improve the ability of plant assimilation NH4+, is conducive to biosynthesis and the accumulation of protein, improves nutritional quality and the proterties of plant; And can excite rapidly the defense response of plant, and open vegeto-animal system of defense, effectively improve the resistance of plant.
Embodiment
Embodiment 1
The walnut early frost antifreezing agent of preparation 1L:
Acetum 1 mL, the mass volume ratio concentration of getting mass volume ratio concentration and be the ethanolic solution 0.01mL of 5% abscisic acid, insect shell glycan that mass volume ratio concentration is 1% is 10% the proline aqueous solution 1 mL, mass volume ratio concentration, and to be 10% aqueous trehalose solution 1 mL, ethanol 100 mL, glycerine 300 mL, surplus be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add successively ethanol, the G & W of above-mentioned quality, fully mix bottling.
Embodiment 2
The walnut early frost antifreezing agent of preparation 1L:
Acetum 0.1 mL, the mass volume ratio concentration of getting mass volume ratio concentration and be ethanolic solution 0.001 mL of 5% abscisic acid, insect shell glycan that mass volume ratio concentration is 1% is 10% proline aqueous solution 0.1mL, mass volume ratio concentration, and to be 10% aqueous trehalose solution 0.1mL, ethanol 50mL, glycerine 100mL, surplus be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add successively ethanol, the G & W of above-mentioned quality, fully mix bottling.
Embodiment 3
The walnut early frost antifreezing agent of preparation 1L:
Acetum 10mL, the mass volume ratio concentration of getting mass volume ratio concentration and be the ethanolic solution 0.1mL of 5% abscisic acid, insect shell glycan that mass volume ratio concentration is 1% is 10% proline aqueous solution 10mL, mass volume ratio concentration, and to be 10% aqueous trehalose solution 10mL, ethanol 300mL, glycerine 500mL, surplus be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add successively ethanol, the G & W of above-mentioned quality, fully mix bottling.
The acetum of the insect shell glycan in above-described embodiment is that insect pressure vessel glycan is dissolved in to mass volume ratio concentration is that in 1% aqueous acetic acid, obtaining insect pressure vessel glycan content is 1%(W/V) the acetum of insect shell glycan.
Embodiment 4
1. mother liquor preparation
(1) plant hormone ABA mother liquor:
Abscisic acid is formulated as 5%(W/V) ethanolic solution.
(2) insect pressure vessel glycan solution preparation:
Insect pressure vessel glycan, is dissolved in 1% aqueous acetic acid, and final concentration is 1%(W/V).
(3) nutrient mother liquor:
Proline, trehalose are formulated as 10% (W/V) aqueous solution, and wherein two kinds of concentration of component are 10%.
2. antifreezing agent
By above mother liquor, mix by following mass volume ratio (W/V) successively: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0 % of insect shell glycan, proline aqueous solution 0.01-1.0 %, aqueous trehalose solution 0.01-1.0 % mix;
Then add 5-30 %(W/V) ethanol, then add 10-50 %(W/V) glycerine, and finally add distilled water to 1L, be antifreezing agent stoste of the present invention.
Raw material in above-described embodiment all can directly be buied from market.
biological activity determination
The embodiment 1-4 of antifreezing agent of the present invention, take walnut as material, sprays antifreezing agent, carries out bud, leaf bud delayed-action and late growing stage rate determination, with the biologically active of clear and definite antifreezing agent of the present invention for about 2 weeks before early spring, walnut bud was sprouted.
(1) antifreezing agent working solution preparation of the present invention:
Reagent agent: antifreezing agent of the present invention
Before antifreezing agent stoste of the present invention uncork, be soaked in 50-60 ℃ of hot water 5 minutes, take out firmly concussion and shaking up, guaranteeing do not have solid to separate out in bottle.With 500 times of running water dilutions, process by following, 1 repetition of 10 every group trees, process 3 repetitions for every kind:
A) control group: the running water that only sprays equivalent.
B) prevent and treat group: only spray antifreezing agent to side shoot and small stems (having bud branch), require to spray evenly, form and be uniformly distributed thin layer on branch surface.(preventing and treating group is 4 groups, respectively is the antifreezing agent of embodiment 1-4)
C) observe day by day the fallen leaves situation of each experiment tree body after spraying; After control group and the whole fallen leaves of control group walnut, the side shoot of clip growth bud, get branch middle part bark and measure pyruvate kinase (Pyruvate Kinase) peroxidase (POD), catalase (CAT) activity relevant to plant freezing resistance, and soluble protein and proline content.
(2) frost resistance measurement result after antifreezing agent dispenser of the present invention:
Antifreezing agent was used after January, added up day by day walnut fallen leaves situation and found that, control group walnut compares according to approximately 15 days in advance whole fallen leaves (table 1), and this illustrates that this invention prescription has and promotes walnut body to fall leaves in advance, enter the effect of dormancy.Walnut enters in advance fallen leaves and enters the dormant stage, just can avoid in the fall or harm that early winter, early frost was sent out from the phenophase.
table 1 walnut antifreezing agent fallen leaves effect
After dispenser January (control group and control group walnut are all fallen leaves), the kinases of control group walnut branch bark, POD, CAT enzymic activity all raise than control group, and soluble protein and proline content are also higher than control group (table 2).Show that the freeze proof related enzyme activity in walnut branch, antifreeze protein level are all than the remarkable rising of contrast after medication, after dispenser, the freezing tolerance of walnut body is improved.
table 2 walnut side shoot bark enzyme activity assay
In sum; we pass through in recent years to the antifreeze research practice of walnut; summing up and utilizing abscisic acid and shitosan is main derivant and fallen leaves conditioning agent; coordinate nutrient, protectant etc.; early frost sprays walnut before occurring in the fall, and metabolism that can be by regulating walnut reaches the effect of falling leaves in advance and producing relevant freezing tolerance to growing.The present invention utilizes the formula combination that the harmless plant hormone of plant, shitosan, nutrient, protectant etc. is combined to form to induction walnut frost resistance; can make in advance walnut fallen leaves; reach the object of avoiding early frost harm; simultaneously; can also induce the freeze proof metabolic pathway of walnut body endogenous, reach the effect of avoiding frost, strengthening frost resistance.
Claims (5)
1. a walnut early frost antifreezing agent, is characterized in that: this walnut early frost antifreezing agent be by the acetum of the ethanolic solution of abscisic acid, insect shell glycan, the proline aqueous solution, aqueous trehalose solution, ethanol, G & W is composite forms.
2. a kind of walnut early frost antifreezing agent according to claim 1, is characterized in that: composite the forming of raw material of the following mass volume ratio of walnut early frost antifreezing agent needs of preparation 1L: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0 % of insect shell glycan, proline aqueous solution 0.01-1.0 %, aqueous trehalose solution 0.01-1.0 %, ethanol 5-30 %, glycerine 10-50 %, surplus are water.
3. a kind of walnut early frost antifreezing agent according to claim 1 and 2, it is characterized in that: the preparation method of described walnut early frost antifreezing agent is: successively the ethanolic solution of the abscisic acid of formula ratio, insect chitosan solution, the proline aqueous solution, aqueous trehalose solution are mixed, and then add successively ethanol, the G & W of formula ratio, fully mix bottling.
4. according to a kind of walnut early frost antifreezing agent described in any one in claim 1,2 or 3, it is characterized in that: described walnut early frost antifreezing agent sprays for 2 weeks before early spring, walnut bud was sprouted.
5. a kind of walnut early frost antifreezing agent according to claim 3, is characterized in that: before described walnut early frost antifreezing agent uncork, be soaked in 50-60 ℃ of hot water 5 minutes, take out firmly concussion and shaking up, guaranteeing do not have solid to separate out in bottle; When use, with 500 times of running water dilutions.
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| CN201310708599.XA CN103875672B (en) | 2013-12-20 | 2013-12-20 | A kind of walnut early frost antifreezing agent |
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| CN201310708599.XA CN103875672B (en) | 2013-12-20 | 2013-12-20 | A kind of walnut early frost antifreezing agent |
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| CN103875672B CN103875672B (en) | 2016-04-20 |
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Cited By (4)
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| CN105272612A (en) * | 2015-10-27 | 2016-01-27 | 丹阳市香逸林果专业合作社 | Anti-freezing nutrient solution for crape myrtle |
| CN105706807A (en) * | 2016-03-01 | 2016-06-29 | 山东省林业科学研究院 | Method for cold resistance of figs in field, cold-resistant agent and preparation method, and cold-resistant cover |
| CN106747905A (en) * | 2016-11-18 | 2017-05-31 | 安徽新视野园林绿化工程有限公司 | A kind of Plant antifreezer with disinsection efficiency and preparation method thereof |
| CN109369271A (en) * | 2018-11-29 | 2019-02-22 | 中国农业科学院棉花研究所 | A kind of antifreezing agent and preparation method and application suitable for wheat |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105272612A (en) * | 2015-10-27 | 2016-01-27 | 丹阳市香逸林果专业合作社 | Anti-freezing nutrient solution for crape myrtle |
| CN105706807A (en) * | 2016-03-01 | 2016-06-29 | 山东省林业科学研究院 | Method for cold resistance of figs in field, cold-resistant agent and preparation method, and cold-resistant cover |
| CN105706807B (en) * | 2016-03-01 | 2018-10-12 | 山东省林业科学研究院 | Fig field cold-resistant method, cold-resistance agent and preparation method, cold-resistant cover |
| CN106747905A (en) * | 2016-11-18 | 2017-05-31 | 安徽新视野园林绿化工程有限公司 | A kind of Plant antifreezer with disinsection efficiency and preparation method thereof |
| CN109369271A (en) * | 2018-11-29 | 2019-02-22 | 中国农业科学院棉花研究所 | A kind of antifreezing agent and preparation method and application suitable for wheat |
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| CN103875672B (en) | 2016-04-20 |
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