CN103875672B - A kind of walnut early frost antifreezing agent - Google Patents
A kind of walnut early frost antifreezing agent Download PDFInfo
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- CN103875672B CN103875672B CN201310708599.XA CN201310708599A CN103875672B CN 103875672 B CN103875672 B CN 103875672B CN 201310708599 A CN201310708599 A CN 201310708599A CN 103875672 B CN103875672 B CN 103875672B
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- antifreezing agent
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Abstract
The invention discloses a kind of walnut early frost antifreezing agent, it is characterized in that: this walnut early frost antifreezing agent be by the acetum of the ethanolic solution of abscisic acid, insect shell glycan, the proline aqueous solution, aqueous trehalose solution, ethanol, G & W is composite forms.The present invention is intended to be occurred by the freeze proof metabolism of induction walnut endogenous, fall leaves in advance and avoid early frost harm, reaches the novel plant antifreezing agent of the anti-early frost effect of walnut.Antifreezing agent of the present invention, under equal temperature condition, walnut can be impelled to shift to an earlier date about 15 days fallen leaves, effectively avoid early frost harm, and the freeze proof metabolism of endogenous induction period simultaneously occurs, and reaches the effect of the anti-early frost of walnut.
Description
Technical field
The invention belongs to plant growth regulator field, relate to a kind of walnut early frost antifreezing agent.Its principle promotes that walnut is fallen leaves, enters resting stage in advance.
Background technology
The mainstay industry that walnut is China's mountain development economy and increases farmers' income, one of main dry fruit export varieties of Ye Shi China.Along with the fast development of rural economy and improving constantly of people's living standard, the nutritive value of walnut and health care are favored by consumers in general.Frost damage is the key factor of restriction China's walnut natural production and harvest, and its impact, much larger than walnut damage by disease and insect, in provinces and regions such as Yunnan, China's walnut main producing region, Shaanxi, Xinjiang, Shanxi, Hebei, all exists walnut frost damage problem.Walnut suffer the general early frost in the fall of frost or spring cold in the late spring occur period, walnut frost damage has very serious implications walnut production, not only cause branch slightly dehydration drain death, flower, leaf, bud are withered to come off, and can cause the significantly underproduction of walnut, even has no harvest.Research walnut frost damage Control Technology, the Walnut tool of prevention to China carrying out walnut frost damage is of great significance.
Summary of the invention
The object of the invention is a kind of walnut early frost antifreezing agent, change the deficiency that traditional plant antifreezing agent generally uses plant hormone.The present invention utilizes nutritive element and plant endo semiochemicals to regulate walnut to grow, and reaches to delay bud and leaf bud is sprouted, promotes the effect of leaf bud, the growth of bud later stage, to avoid the frostbite of early frost to the sprout that sprouts, reaches the effect of anti-early frost.
Technical scheme of the present invention is: a kind of walnut early frost antifreezing agent, is characterized in that: this walnut early frost antifreezing agent be by the acetum of the ethanolic solution of abscisic acid, insect shell glycan, the proline aqueous solution, aqueous trehalose solution, ethanol, G & W is composite forms.
The walnut early frost antifreezing agent of preparation 1L needs that the raw material of following mass volume ratio is composite to be formed: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0% of insect shell glycan, proline aqueous solution 0.01-1.0%, aqueous trehalose solution 0.01-1.0%, ethanol 5-30%, glycerine 10-50%, surplus are water.
The preparation method of described walnut early frost antifreezing agent is: the ethanolic solution of the abscisic acid of formula ratio, insect chitosan solution, the proline aqueous solution, aqueous trehalose solution are mixed successively, and then add ethanol, the G & W of formula ratio successively, fully mix and bottle.
Described walnut early frost antifreezing agent sprays for 2 weeks before early spring, walnut bud was sprouted.
To be soaked in 50-60 DEG C of hot water 5 minutes before described walnut early frost antifreezing agent uncork, to take out firmly concussion and shake up, guarantee in bottle, do not have solid to separate out; During use, dilute 500 times with running water.
Advantage of the present invention is: antifreezing agent of the present invention has delay walnut bud, leaf bud sprouts effect, and delay bud, leaf bud sprout time reach 10 ~ 15 days, can reach the effect avoiding early frost harm, thus reach anti-early frost, antifreeze effect.Meanwhile, the trace element in formula and shitosan also can excite other degeneration-resistant reaction of plant further and promote not have a negative impact the late growing stage of bud, leaf bud to the growth of walnut body.Compare with traditional Plant antifreezer, this prescription is not containing any plant hormone class growth regulator, and character is gentle, do not cause interference to growth and development of plants, belongs to trophism regulating plant resistance and retarded germination effect.Wherein, abscisic acid (ABA) is ubiquitous natural materials in plant, the mankind eat fruit, vegetables, all naturally in grain to contain, to the mankind and Environmental security.Abscisic acid is a kind of plant hormone with sequiterpene structure, and abscisic acid can promote the accumulation of the reserve substance of seed, fruit, particularly storage protein and sugared part; Vital effect is play to inducing plant resistance.Shitosan has obvious physiological regulation function to plant ammonia assimilation key enzyme, can improve the ability of plant assimilating NH4+, is conducive to biosynthesis and the accumulation of protein, improves nutritional quality and the proterties of plant; And the defense response of plant can be excited rapidly, open vegeto-animal system of defense, effectively improve the resistance of plant.
Embodiment
Embodiment 1
The walnut early frost antifreezing agent of preparation 1L:
Get ethanolic solution 0.01mL that mass volume ratio concentration is the abscisic acid of 5%, aqueous trehalose solution 1mL, ethanol 100mL, glycerine 300mL, surplus that proline aqueous solution 1mL, mass volume ratio concentration that acetum 1mL that mass volume ratio concentration is the insect shell glycan of 1%, mass volume ratio concentration are 10% are 10% be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add ethanol, the G & W of above-mentioned quality successively, fully mix and bottle.
Embodiment 2
The walnut early frost antifreezing agent of preparation 1L:
Get ethanolic solution 0.001mL that mass volume ratio concentration is the abscisic acid of 5%, aqueous trehalose solution 0.1mL, ethanol 50mL, glycerine 100mL, surplus that proline aqueous solution 0.1mL, mass volume ratio concentration that acetum 0.1mL that mass volume ratio concentration is the insect shell glycan of 1%, mass volume ratio concentration are 10% are 10% be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add ethanol, the G & W of above-mentioned quality successively, fully mix and bottle.
Embodiment 3
The walnut early frost antifreezing agent of preparation 1L:
Get ethanolic solution 0.1mL that mass volume ratio concentration is the abscisic acid of 5%, aqueous trehalose solution 10mL, ethanol 300mL, glycerine 500mL, surplus that proline aqueous solution 10mL, mass volume ratio concentration that acetum 10mL that mass volume ratio concentration is the insect shell glycan of 1%, mass volume ratio concentration are 10% are 10% be water.
Successively the acetum of the ethanolic solution of the abscisic acid of above-mentioned quality, insect shell glycan, the proline aqueous solution, aqueous trehalose solution are mixed, and then add ethanol, the G & W of above-mentioned quality successively, fully mix and bottle.
The acetum of the insect shell glycan in above-described embodiment is that insect pressure vessel glycan is dissolved in mass volume ratio concentration is in the aqueous acetic acid of 1%, and obtaining insect pressure vessel glycan content is 1%(W/V) the acetum of insect shell glycan.
Embodiment 4
1. mother liquor
(1) plant hormone ABA mother liquor:
Abscisic acid is formulated as 5%(W/V) ethanolic solution.
(2) insect pressure vessel glycan solution preparation:
Insect pressure vessel glycan, is dissolved in the aqueous acetic acid of 1%, and final concentration is 1%(W/V).
(3) nutrient mother liquor:
Proline, trehalose are formulated as 10%(W/V) aqueous solution, wherein two kinds of concentration of component are 10%.
2. antifreezing agent
By above mother liquor, mix by following mass volume ratio (W/V) successively: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0% of insect shell glycan, proline aqueous solution 0.01-1.0%, aqueous trehalose solution 0.01-1.0% mix;
Then 5-30%(W/V is added) ethanol, then add 10-50%(W/V) glycerine, finally add distilled water to 1L, be antifreezing agent stoste of the present invention.
Raw material in above-described embodiment all can directly be buied from market.
biological activity determination
The embodiment 1-4 of antifreezing agent of the present invention take walnut as material, within about 2 weeks before early spring, walnut bud was sprouted, sprays antifreezing agent, carries out bud, leaf bud delayed-action and late growing stage rate determination, with the biologically active of clear and definite antifreezing agent of the present invention.
(1) antifreezing agent working solution preparation of the present invention:
Reagent agent: antifreezing agent of the present invention
To be soaked in 50-60 DEG C of hot water 5 minutes before antifreezing agent stoste of the present invention uncork, to take out firmly concussion and shake up, guarantee in bottle, do not have solid to separate out.Dilute 500 times with running water, by following process, often organize 1 repetition of 10 trees, often kind of process 3 repetitions:
A) control group: the running water only spraying equivalent.
B) prevent and treat group: only spray antifreezing agent to side shoot and small stems (having bud branch), require that spraying evenly, in the formation of branch surface is uniformly distributed thin layer.(preventing and treating group is 4 groups, respectively is the antifreezing agent of embodiment 1-4)
C) the fallen leaves situation of each experiment tree body is observed after spraying day by day; After all falling leaves Deng control group and control group walnut, the side shoot of clip growth bud, get bark in the middle part of branch and measure pyruvate kinase (PyruvateKinase) peroxidase (POD) relevant to plant freezing resistance, catalase (CAT) activity, and soluble protein and proline content.
(2) frost resistance measurement result after antifreezing agent dispenser of the present invention:
After January used by antifreezing agent, add up walnut fallen leaves situation day by day and found that, control group walnut compares according to about 15 days in advance whole fallen leaves (table 1), and this illustrates that this invention prescription has and promotes walnut body to fall leaves in advance, enter the effect of dormancy.Walnut enters fallen leaves in advance and enters the dormant stage, just can avoid in the fall or harm that early winter, early frost was sent out from the phenophase.
table 1 walnut antifreezing agent fallen leaves effect
After dispenser January (control group and control group walnut are all fallen leaves), kinases, POD, CAT enzymic activity of control group walnut branch bark all raise than control group, and soluble protein and proline content are also higher than control group (table 2).Freeze proof related enzyme activity after showing medication in walnut branch, antifreeze protein level are all than contrasting remarkable rising, and after dispenser, the freezing tolerance of walnut body is improved.
table 2 walnut side shoot bark enzyme activity assay
In sum; we pass through in recent years to the antifreeze research practice of walnut; sum up and utilize abscisic acid and shitosan to be primary inducers and fallen leaves conditioning agent; coordinate nutrient, protectant etc.; early frost sprays walnut before occurring in the fall, can fall leaves in advance and produce the effect of relevant freezing tolerance to growing to reach by regulating the metabolism of walnut.The present invention utilizes the formula combination plant hormone, shitosan, nutrient, protectant etc. of non-phytotoxic being combined to form to induction walnut frost resistance; can walnut be made in advance to fall leaves; reach the object avoiding early frost harm; simultaneously; the freeze proof metabolic pathway of walnut body endogenous can also be induced, reach the effect avoided frost, strengthen frost resistance.
Claims (3)
1. a walnut early frost antifreezing agent, is characterized in that: this walnut early frost antifreezing agent be by the acetum of the ethanolic solution of abscisic acid, insect shell glycan, the proline aqueous solution, aqueous trehalose solution, ethanol, G & W is composite forms; The walnut early frost antifreezing agent of preparation 1L needs that the raw material of following mass volume ratio is composite to be formed: the ethanolic solution of 0.0001-0.01% abscisic acid, the acetum 0.01-1.0% of insect shell glycan, proline aqueous solution 0.01-1.0%, aqueous trehalose solution 0.01-1.0%, ethanol 5-30%, glycerine 10-50%, surplus are water; The preparation method of described walnut early frost antifreezing agent is: the ethanolic solution of the abscisic acid of formula ratio, insect chitosan solution, the proline aqueous solution, aqueous trehalose solution are mixed successively, and then add ethanol, the G & W of formula ratio successively, fully mix and bottle.
2. a using method for walnut early frost antifreezing agent as claimed in claim 1, is characterized in that: described walnut early frost antifreezing agent sprays for 2 weeks before early spring, walnut bud was sprouted.
3. a using method for walnut early frost antifreezing agent as claimed in claim 1, is characterized in that: to be soaked in 50-60 DEG C of hot water 5 minutes before described walnut early frost antifreezing agent uncork, takes out firmly concussion and shakes up, guarantees do not have solid to separate out in bottle; During use, dilute 500 times with running water.
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| CN201310708599.XA CN103875672B (en) | 2013-12-20 | 2013-12-20 | A kind of walnut early frost antifreezing agent |
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| CN201310708599.XA CN103875672B (en) | 2013-12-20 | 2013-12-20 | A kind of walnut early frost antifreezing agent |
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| CN103875672A CN103875672A (en) | 2014-06-25 |
| CN103875672B true CN103875672B (en) | 2016-04-20 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105272612A (en) * | 2015-10-27 | 2016-01-27 | 丹阳市香逸林果专业合作社 | Anti-freezing nutrient solution for crape myrtle |
| CN105706807B (en) * | 2016-03-01 | 2018-10-12 | 山东省林业科学研究院 | Fig field cold-resistant method, cold-resistance agent and preparation method, cold-resistant cover |
| CN106747905A (en) * | 2016-11-18 | 2017-05-31 | 安徽新视野园林绿化工程有限公司 | A kind of Plant antifreezer with disinsection efficiency and preparation method thereof |
| CN109369271A (en) * | 2018-11-29 | 2019-02-22 | 中国农业科学院棉花研究所 | A kind of antifreezing agent and preparation method and application suitable for wheat |
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| CN1613322A (en) * | 2003-11-03 | 2005-05-11 | 武兴战 | Sugar biological composite pisatin with nanometer structure and preparation thereof |
| CN102090395A (en) * | 2011-01-05 | 2011-06-15 | 贵州省烟草科学研究所 | Abscisic acid-embedded chitosan nanoparticles and preparation method thereof |
| CN102326545A (en) * | 2011-06-20 | 2012-01-25 | 中国农业大学 | Drought-resistant agent and application thereof |
| CN103238629A (en) * | 2013-05-02 | 2013-08-14 | 浙江师范大学 | Cold-resistant agent for growth of Chinese trichosanthes and using method thereof |
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