CN102326545A - Drought-resistant agent and application thereof - Google Patents
Drought-resistant agent and application thereof Download PDFInfo
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- CN102326545A CN102326545A CN201110166528A CN201110166528A CN102326545A CN 102326545 A CN102326545 A CN 102326545A CN 201110166528 A CN201110166528 A CN 201110166528A CN 201110166528 A CN201110166528 A CN 201110166528A CN 102326545 A CN102326545 A CN 102326545A
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Abstract
The invention discloses a drought-resistant agent and application thereof. The plant drought-resistant agent comprises the active ingredients such as coronatine (COR) and S-abscisic acid (S-ABA). Compared with the present substance for improving the drought resistance of crops, the drought-resistant agent has the following characteristics: unexpected synergy can be obtained through COR, S-ABA and other plant growth adjusting substances which are added on the basis of the COR and S-ABA; an expected addition effect on the growth of crops and the adjustment of the activities can be exceeded, and on one hand, the crop application range can be enlarged, and on the other hand, the application concentration of coronatine, S-ABA and other plant growth adjusting agents can be reduced; and moreover, under the condition that the concentration of a single compound is lower than the effective concentration, a good adjusting degree on the growth of plants can be obtained through the plant growth adjusting agent.
Description
Technical field
The present invention relates to a kind of drought resistance agent and application thereof.
Background technology
In recent years, along with global warming, the large tracts of land arid is frequent all over the world takes place, and water resources problems becomes increasingly conspicuous, and the nervous situation of China's water resource is particularly severe.According to statistics; China per capita the not enough world average level of freshwater resources occupancy volume 1/4; And the spatial and temporal distributions of water resource is extremely uneven, is ploughing and population accounts for respectively in 15 provinces and regions, the north of 45% and 38% of national total amount, and water resource only accounts for 9.7% national (Jiang and equality; Chinese development is observed, 2009:28-30).Water resources shortage influences crop growth, causes the underproduction, when serious even cause the crop total crop failure.It is reported, the crop loss that drought causes be equivalent to other various natural calamities damnous comprehensive (Ding Shengjun, Chinese food and nutrition, 2009,4:4-6).In 59 years of statistics are arranged, arid disaster area account for total natural calamity disaster area ratio 56.6 (Gu Ying etc., the water conservancy and hydropower technology, 2010,1:71-74).
(Coronatine is a kind of nosotoxin that pseudomonad produces COR) to coronatine, and it is a kind of cyclopentane structure analog of jasmine acids plant hormone, and structural formula is following.
Natural abscisic acid, S-Abscisic acid (S-ABA), structural formula see as follows, the agricultural chemicals common name: the S-abscisic acid.
Summary of the invention
An object of the present invention is to provide a kind of plant drought-resisting agent.
Plant drought-resisting agent provided by the present invention is 1) or 2) shown in:
1) its active component is COR and S-ABA;
2) form by COR and S-ABA.
In the above-mentioned plant drought-resisting agent, the mass ratio of said COR and S-ABA is (0.01-0.1): 1, be specially 0.01: 1 or 0.1: 5 or 0.1: 1.
Also can comprise plant growth regulating substance in above-mentioned arbitrary said plant drought-resisting agent.
In above-mentioned arbitrary said plant drought-resisting agent, the quality proportioning of said COR, S-ABA and plant growth regulating substance is: 1: (10-100): (10-100000) or 1: (10-100): (100-100000) or 1: (10-100): (100-30000); Be specially 1: (10-100): (10-50000), 1: (10-100): (20-50000), 1: (10-100): (500-50000) or 1: (10-100): (5000-50000); Be specially again 1: 10: (20-1000), 1: 50: (10-5000), 1: 50: (500-5000) or 1: 100: (20-50000); Especially be specially 1: 10: 20,1: 10: 100,1: 10: 250,1: 10: 1000,1: 50: 10,1: 50: 500,1: 50: 2000,1: 50: 5000,1: 100: 20,1: 100: 20000,1: 100: 30000 or 1: 100: 50000.
In above-mentioned arbitrary said plant drought-resisting agent, said plant drought-resisting agent comprises water.
In above-mentioned arbitrary said plant drought-resisting agent, the concentration of said COR in said drought resistance agent is 0.01-10.0mg/L or 0.01-1mg/L; Be specially 0.01mg/L-0.5mg/L or 0.1-2.0mg/L, be specially 0.01mg/L, 0.1mg/L, 0.2mg/L or 0.5mg/L again;
The concentration of said S-ABA in said drought resistance agent is 1.0-100mg/L or 1.0-50.0mg/L or 1.0-20.0mg/L; Be specially 1.0mg/L-5.0mg/L; Be specially 1.0mg/L, 2.0mg/L or 5.0mg/L again;
The concentration of said plant growth regulating substance in said drought resistance agent is 0.2-1000mg/L, is specially 0.2,1.0,50,100,200,300,500 or 1000mg/L.
In above-mentioned arbitrary said plant drought-resisting agent, said plant growth regulating substance is at least a in following: fulvic acid, shitosan, amino-oligosaccharide, brassin lactones, first piperazine, uniconazole P, ethrel and Prohexadione calcium.
Wherein, the number-average molecular weight Mn=1032 of said fulvic acid; The weight average molecular weight of said shitosan: Mw=300000-600000; The weight average molecular weight Mw=300-3000 of said amino-oligosaccharide.
In above-mentioned arbitrary said plant drought-resisting agent, said plant drought-resisting agent also can comprise required carrier and the auxiliary agent of preparation formulation; The formulation of said drought resistance agent is specially missible oil, soluble powder, suspending agent, wetting powder, granule, extrusion type granula or microcapsule formulations.
In above-mentioned arbitrary said plant drought-resisting agent, said plant is crops, is specially wheat, corn, soybean, cotton or paddy rice etc.
The application of above-mentioned arbitrary said plant drought-resisting agent in promoting plant drought also belongs to protection of the present invention.
In the above-mentioned application, said plant is crops, is specially wheat, corn, soybean, cotton or paddy rice etc.
The present invention also protects the method for using of above-mentioned arbitrary said plant drought-resisting agent.
Said method for using comprises the steps: the said plant drought-resisting agent of dilute with water, makes the concentration of said COR, S-ABA and plant growth regulating substance satisfy following condition, uses again;
The concentration of said COR is 0.01-10.0mg/L or 0.01-1mg/L; Be specially or 0.01mg/L-0.5mg/L or 0.1-2.0mg/L; Be specially 0.01mg/L, 0.1mg/L, 0.2mg/L or 0.5mg/L again;
The concentration of said S-ABA is 1.0-100mg/L or 1.0-50.0mg/L or 1.0-20.0mg/L; Be specially 1.0mg/L-5.0mg/L; Be specially 1.0mg/L, 2.0mg/L or 5.0mg/L again;
The concentration of said plant growth regulating substance is 0.2-1000mg/L; Be specially 0.2,1.0,50,100,200,300,500 or 1000mg/L.
Plant growth regulating substance is through promoting that plant root growth promotes moisture absorption, and shitosan itself also has anti-transpiration, and amino-oligosaccharide is a kind of immunomodulator, can improve the immunocompetence of plant, stimulating plant growth effect.
The application dosage of drought resistance agent of the present invention can change in quite wide scope, selects according to actual needs such as vegetative period of the kind of using processing means (foliage-spray, smear, irritate root, seed dressing, injection etc.), cultivated plant, plant, weather conditions.Usually, the using dosage of drought resistance agent is 1.0-1500 gram active component/hectare according to the present invention.The application dosage of plant growth regulator composition is not crucial during use, and coronatine concentration should remain on 0.01-10mg/L during crucial being to use, and natural abscisic acid should remain on 1-100mg/L.
Compare with the existing crop drought resistance material that improves, drought resistance agent of the present invention has following characteristics:
1, COR and S-ABA and add other plant growth regulating substance on this basis and can obtain unexpected synergy; The addition that the growth regulating-activity of crop is surpassed expection; Mainly be to have improved the crop scope of application on the one hand; Reduced the working concentration of coronatine, S-ABA and other plant growth regulator on the other hand, and the combination of this plant growth regulator also, single compound obtains good plant growth regulating level under being lower than the situation of valid density.
2, biologically active is high, and per hectare is used and can be low to moderate below 1.0 grams;
3, can improve resistivity to abiotic stresses such as arid, ultraviolet and lodging;
4, the two all belongs to natural products, and Environmental compatibility is good, does not have residual residual effect problem.
Description of drawings
Fig. 1 is the liquid chromatogram collection of illustrative plates of coronatine standard items.
Fig. 2 is the liquid phase chromatic graph spectrum that makes the coronatine sample.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Wheat breed Shan 253, authorization (registration) is numbered Shan and examines wheat 2001004.
The wheat breed capital winter No. 8, be numbered state and examine wheat 990008.
Corn variety agricultural university 3138 is numbered state and examines beautiful 980004.
Soybean varieties is cultivated farming 33, is numbered black cultivating and examines beans 2009002.
Anti-No. 12 of Insect Resistant Cotton kind state, available from the glad rural area technological service of Hejian City state always, production code member GK-12.
Rice varieties is cultivated rice 12, is numbered the black rice 2006009 of examining.
Coronatine (COR) prepares according to method described in the embodiment 1.
Natural abscisic acid (S-ABA) is available from LongMang Fusheng Technology Co., Ltd, Sichuan, and product is called " the solvable pulvis of 1%S-abscisic acid ", agriculture chemical registration card PD20093848.
Said fulvic acid is built first agricultural science and technology Co., Ltd available from Henan, the total acidic group 8.76 milliequivalent/grams of product " purification fulvic acid (95%) " by name, number-average molecular weight: Mn=1032.
Said shitosan really becomes chemical company available from Korea S, and product is called " 2% chitosan aqua " (commodity " playing local products " by name); Weight average molecular weight: Mw=300000-600000.
Said amino-oligosaccharide is available from Hainan Zhengye Zhongnong Hi-Tech Industry Co., Ltd, and product is called " 5% amino-oligosaccharide aqua ", agriculture chemical registration card LS20110014; Weight average molecular weight: Mw=300-3000.
The CAS accession number of said brassin lactones is 72962-43-7, and available from new Chaoyang, Chengdu biochemistry corporation,Ltd., product is called " the solvable pulvis of 0.1% brassin lactones ", agriculture chemical registration card PD20070288.
The CAS accession number of said first piperazine is 15302-91-7, and available from Great Wall, Zhangjiakou, Hebei province farmingization (group) Co., Ltd, product is called " the solvable pulvis of 98% first piperazine ", agriculture chemical registration card PDN39-96.
The CAS accession number of said uniconazole P is 83657-22-1, and available from Jiangsu Qizhou Green Chemical Co., Ltd, product is called " 5% uniconazole P wetting powder ", agriculture chemical registration card PD20070352.
The CAS accession number of said ethrel is 16672-87-0, and available from Jiangsu Anpon Electrochemical Co., Ltd., product is called " 40% ethephon agent ", agriculture chemical registration card PD84125-3.
The CAS accession number of said Prohexadione calcium is 127277-53-6, and available from Yizailing Agriculture Science and Technology Co., Ltd., Hubei, product is called " 5% Prohexadione calcium effervescence tablet agent ", agriculture chemical registration card LS20100132.
Compositions-treated all is to use to make up behind the various one-components of distilled water diluting and obtains among the embodiment 2-7.
Embodiment 1, preparation coronatine
Coronatine (COR) standard items are available from Sigma-Aldrich company, content＞95%, production code member C8115-1MG.
The bacterium pseudomonas syringae soybean of will recombinating is caused a disease mutation MW123 (available from CGMCC; Preserving number CGMCCNo.2533) is seeded in the MG liquid nutrient medium that contains the streptomycin that kanamycin that final concentration is 10 μ g/L and final concentration be 30 μ g/L; In temperature is 28 ℃; Cultivated 48 hours on the shaking table of rotating speed 260rpm, obtain primary seed solution.
The MG liquid nutrient medium is formed as follows:
Solid culture medium: add 1.5% agar powder
Sterilized 20 minutes for 121 ℃
The HSC medium is formed as follows:
A liquid:
B liquid:
Glucose 20g
Distilled water 10mL
A liquid was 115 ℃ of sterilizations 15 minutes, and B liquid was 121 ℃ of sterilizations 20 minutes
600ml MG liquid nutrient medium is sub-packed in three 500ml triangular flasks, and promptly every bottle contains the 200ml medium; The primary seed solution that cultivation is obtained is inoculated in above-mentioned three 500ml triangular flasks respectively by the 1%-2.5% inoculum concentration, is 28 ℃ in temperature, cultivates 48 hours on the shaking table of rotating speed 260rpm, obtains secondary seed solution.
To be loaded on through 5 liters of HSC medium of conventional hot, high pressure method for steam sterilization sterilization in 7 liters the fermentation tank; By the 1%-2.5% inoculum concentration to wherein inserting secondary seed solution; Air agitation is that 18 ℃, initial pH are to ferment under the condition of 6.0-7.0 in temperature, and fermentation time reaches 7 days at present jar.
Get the zymotic fluid 1000ml that makes, centrifugal 20min under 4 ℃ of conditions collects supernatant, and with the hydrochloric acid of 1.0M the pH value of supernatant is transferred to 2.5; With the supernatant that obtains with ethyl acetate extraction 3 times (120ml ethyl acetate: the 100ml supernatant), discard water, collect organic facies; Organic facies is concentrated into 15ml under 35 ℃, with concentrate 3 (15ml sodium carbonate liquors: the 15ml concentrate), discard organic facies, collect water of sodium carbonate liquor extraction with the 50mM of pH 10.5; Water is transferred to pH 2.5, (the 15ml: 15ml ethyl acetate), discard water, collect organic facies that uses ethyl acetate extraction then 3 times; Organic facies is concentrated evaporate to dryness, suspend, get 0.5ml and be used for the high performance liquid chromatography detection with 20ml methyl alcohol.The flowing phase of using during high performance liquid chromatography detects is mixed with by 0.05% trifluoroacetic acid solution (pH 2.9) and the acetonitrile ratio in 1: 9.Testing sample carries out under the condition of the speed that detects wavelength 208nm place, 2ml/min, sample size 25 μ l, each sample reservation 10min.Confirm whether contain coronatine in the extract according to coronatine standard items retention time, and through confirming the content of coronatine with coronatine standard items peak area ratio.
Under above testing conditions, the retention time of coronatine is 7.7 minutes, and the collection of illustrative plates of coronatine standard items is as shown in Figure 1, and the collection of illustrative plates of laboratory sample is as shown in Figure 2.3 repetitions are established in experiment.The result show coronatine and average yield is the 200.5mg/L zymotic fluid.
According to the coronatine content that records, add methyl alcohol and be deployed into 5% coronatine soluble concentrate, be provided with down embodiment and use.
Embodiment 2, the test of the drought resisting on wheat seeds sprouting
Wheat breed is Shan 253, for moisture is compared sensitive varieties.The seed of choosing uniformity was with 10% aqueous hydrogen peroxide solution soaking disinfection 10 minutes; Distilled water flushing 5 times, the medicament seed soaking 12 hours that places distilled water diluting is more respectively transferred in the plastic casing with cover (12 * 15 centimetres) with 3 metafiltration paper of 15 milliliters of 10%PEG-6000 solution-wet; 100 in every box; Place under the 20 degree climatic cabinate dark conditions and carry out germination test, each is handled 4 times and repeats, and the seed of arid blank is without the medicament immersion treatment; The clear water blank does not add PEG-6000 and chemicals treatment.Respectively at handling beginning back the 4th day statistics germination vigor and the 7th day statistics germination rate; Picked at random 25 strain seedling are measured height of seedling, single-strain fresh weight, total dry weight after 7 days; Picked at random 10 strain seedling are used EPSON EXPRESSION
TM1680 root system scanners and Win RHIZO analysis software are measured the long and root system surface area (result sees table 2) of total root.
Equate with seed length or radicel length reaches seed length one half as the germination standard with the wheat radicle length; Account for the percentage of handling seed number with the 4th day chitting piece number and calculate germination vigor, the 7th day chitting piece number accounts for the percentage of handling seed number and calculates germination rate.
The drought resisting effect of table 2 on wheat seeds sprouting
The result shows, after using the present composition and handling, can promote the growth of wheat seedling under manual simulation's the drought condition, has significant drought resisting effect, and its effect is superior to the independent use of two active ingredients.
Embodiment 3, the drought resisting test of between wheat paddock, producing
Material is a wheat, and kind is capital winter No. 8.Test in 2009 in the Haidian District, Beijing City Shang Zhuan township China Agricultural University experiment base carry out, soil is loam.Pour water in breeding time and mainly adopt the filling furrow mode of flexible pipe water-conveying to the field, irrigation quantity is 900 cubes/hectare, and " not watering ", " watering winter water " and " watering winter water+grouting water " three kinds of arid modes are set, 4 repetitions, investigation cell production during results.The medicament list is set to be used and the clear water blank.30 square metres of sub-district areas, carry out on the processing employing blade face evenly mode of spraying, and chemicals treatment and concentration are seen table 3.
The group of " not watering ": carry out the chemicals treatment first time at period of seedling establishment, amount of liquid medicine is 225 liters/hectare; Carry out the chemicals treatment second time in the watery stage, amount of liquid medicine is 450 liters/hectare.
" water winter water " and organize: carry out chemicals treatment in the watery stage, amount of liquid medicine is 450 liters/hectare.
" water winter water+grouting water " and organize: carry out chemicals treatment in the shooting stage, amount of liquid medicine is 450 liters/hectare.
The result is as shown in table 3.
The result shows, use patent compositions-treated of the present invention after, can improve the output of wheat under the drought condition, and arid better effects if when more serious, therefore have significant drought resisting effect, its effect is superior to the independent use of two active ingredients.
The drought resisting effect that table 3 is produced between wheat paddock
Explain: breeding time normal irrigation (winter water, be in the milk water and choose flag water each 1 time) cell production is 16.08kg.
Embodiment 4, in the drought resisting of growth of maize test
Material is a corn, and kind is an agricultural university 3138.Test that China Agricultural University experiment greenhouse carried out in the Haidian District, Beijing City in 2008.The seed of choosing uniformity soaks with 1% liquor natrii hypochloritis and carried out disinfection in 10 minutes; After repeatedly washing with running water again, 25 ℃ of seed soaking 24 hours, be seeded in the plastic casing that contains the 6.5kg sandy soil (25cm * 18cm * 20cm); Plastic casing wraps up with masking foil; The sandy soil dress box of after cleaning oven dry, weighing is mixed fertilizer by every box 0.5,0.5 of nitrogen phosphorus potassium and 0.75kg, and every box quantitatively waters and keeps soil moisture content about 0.08g/g soil; Wait to grow to leaf final singling wholeheartedly the time behind the insemination and emergence, at 25 ℃ of room temperatures, 400 μ mol/m
2S illumination 12h/d cultivates down, grows to 3 when launching leaves, carry out the medicament foliar spray and handle, to blade surface spray drop evenly not dirty till.The method of (2005) such as arid analog references Hattori is carried out, and arid is handled and kept about soil moisture content 0.03g/g soil, and every afternoon is the weighing moisturizing regularly.Promptly carry out the arid processing after the chemicals treatment and be maintained until spraying back 20 days, every processing repetition 4 times.The consistent milpa of 10 strains growth is got in every processing, measures morphological indexs (result sees table 4) such as its plant height, main root length, radical, seedling fresh weight and seedling dry weight.
The result shows, after using the present composition and handling, improves the drought resistance of corn through promoting root growth, therefore has significant drought resisting effect, and its effect is superior to the independent use of two active ingredients.
The drought resisting effect of table 4 on growth of maize
Embodiment 5, the test of the drought resisting on soybeans they grow
Material is a soybean, and kind is for cultivating No. 33, farming.Test in 2010 in the Haidian District, Beijing City China Agricultural University's solarium's pot experiment carry out.The diameter 25cm of plastic basin, high 30cm.Potting soil contains organic matter 2.5%, full nitrogen 0.17%, alkali-hydrolyzable nitrogen 182mg/kg, rapid available phosphorus 85mg/kg, available potassium 205mg/kg.Every basin dress 15kg soil is placed in the solarium that removable cloth for keeping out rain is housed.8 of every basin sowings are 4 strains in V3 phase (three joint phases) field planting, precedingly carry out normal water management in the R3 phase (pod phase beginning).The medicament foliage-spray is handled and is carried out in the soybean R1 phase (early flowering season), and amount of liquid medicine is 450 liters/hectare; Contrast is handled for clear water.Arid is handled and is carried out in the R3 phase; The normal water branch is remained on soil relative water content 75%; Arid moisture remains on soil relative water content 40%; Soil moisture content is controlled with weight method, and each handles repetition 20 basins, measures during results that individual plant root dry weight, root nodule are heavy, cauline leaf dry weight and grain heavy (result sees table 5).
The result shows, use patent compositions-treated of the present invention after, through promoting the growth of root system and root nodule, improved the drought resistance of soybean, effect is remarkable, its effect is superior to the independent use of two active ingredients.
The drought resisting effect of table 5 on soybeans they grow
Embodiment 6, the test of the drought resisting on cotton growth
Material is an Insect Resistant Cotton, and kind is anti-No. 12 of state.Tested in 2010 and carry out in experiment station, China Agricultural University Wuqiao.The experimental field is a sandy loam, and middle fertility contains organic 11.6mg/kg, alkali-hydrolyzable nitrogen 33.1mg/kg, available phosphorus 17.3mg/kg, effective potassium 101.5mg/kg.Sowing on April 20,6 row districts, long 10.0m, wide 4.5m.The plantation of size row, wide row space 1.0m, little line-spacing 0.5m, density 60,000 strains/ha.Carry out normal water management before the initial bloom stage, and carry out the medicament foliar treatment 1 time, amount of liquid medicine 450m
3/ ha, and carry out the clear water control treatment.Carry out arid at full-bloom stage and handle, irrigation quantity is 900m during normal water management
3/ ha, arid is treated to 300m
3/ ha.Randomised block design repeats 4 times.The bell number is investigated in consistent cotton plant 10 strains of internal labeling growth in each sub-district during results, and it is heavy to take by weighing bell after air-dry, the whole unginned cottons' results in each sub-district weigh (result sees table 6).
The result shows, after using the present composition and handling, improved individual plant bell number and the bell weight the drought condition under, and the antithetical phrase ginnings improves, and shows tangible drought resisting effect, and its effect is superior to the independent use of two active ingredients.
The drought resisting effect of table 6 on cotton growth
Embodiment 7, the test of the drought resisting on paddy growth
Material is a paddy rice, and kind is for cultivating rice 12.Tested in 2009 and carry out in No.850 Farm of Heilongjiang Prov. paddy rice scientific and technological park.Soil is the grassy marshland white muddy soil, and about topsoil 20cm, soil fertility is medium, soil organic matter content 3.9%, pH value about 6.0.By normal water management, arid is treated to that irrigation quantity is 50% of normal water management between the shooting stage before shooting stage.Chemicals treatment is carried out foliar spray 1 time in the shooting stage, and amount of liquid medicine is 450L/ha, and establishes the clear water contrast.Randomised block design is adopted in test, repeats 4 times.Every sub-district 15 row, the long 10.0m of row, line-spacing 0.35m, area 52.5m
2Maturing stage examines or check, investigation grain number per spike, thousand kernel weight, and results cell production (result sees table 7).
The result shows that the present composition can improve the drought resistance of paddy rice after handling, and its effect is superior to the independent use of two active ingredients.
The drought resisting effect of table 7 on paddy growth
Claims (10)
1. plant drought-resisting agent, its active component is COR and S-ABA.
2. plant drought-resisting agent according to claim 1 is characterized in that: the mass ratio of said COR and S-ABA is (0.01-0.1): 1.
3. plant drought-resisting agent according to claim 1 and 2 is characterized in that: said plant drought-resisting agent comprises plant growth regulating substance.
4. plant drought-resisting agent according to claim 3 is characterized in that: the quality proportioning of said COR, S-ABA and plant growth regulating substance is: 1: (10-100): (10-100000) or 1: (10-100): (100-100000) or 1: (10-100): (100-30000).
5. according to arbitrary described plant drought-resisting agent among the claim 1-4, it is characterized in that: said plant drought-resisting agent comprises water.
6. plant drought-resisting agent according to claim 5 is characterized in that:
The concentration of said COR in said drought resistance agent is 0.01-10.0mg/L or 0.01-1mg/L;
The concentration of said S-ABA in said drought resistance agent is 1.0-100mg/L or 1.0-50.0mg/L or 1.0-20.0mg/L;
The concentration of said plant growth regulating substance in said drought resistance agent is 0.2-1000mg/L.
7. according to arbitrary described plant drought-resisting agent among the claim 3-6, it is characterized in that: at least a in following of said plant growth regulating substance: fulvic acid, shitosan, amino-oligosaccharide, brassin lactones, first piperazine, uniconazole P, ethrel and Prohexadione calcium.
8. according to arbitrary described plant drought-resisting agent among the claim 1-7, it is characterized in that: said plant drought-resisting agent comprises required carrier and the auxiliary agent of preparation formulation; The formulation of said drought resistance agent is specially missible oil, soluble powder, suspending agent, wetting powder, granule, extrusion type granula or microcapsule formulations.
9. the application of arbitrary said plant drought-resisting agent in promoting plant drought among the claim 1-8.
10. the method for using of arbitrary described plant drought-resisting agent among the claim 1-8 comprises the steps: the said plant drought-resisting agent of dilute with water, makes the concentration of said COR, S-ABA and plant growth regulating substance satisfy following condition, uses again;
The concentration of said COR is 0.01-10.0mg/L or 0.01-1mg/L;
The concentration of said S-ABA is 1.0-100mg/L or 1.0-50.0mg/L or 1.0-20.0mg/L;
The concentration of said plant growth regulating substance is 0.2-1000mg/L.
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| CN114304159A (en) * | 2021-12-28 | 2022-04-12 | 石河子大学 | Medicament for improving stress resistance of desert oasis protection forest in western arid region |
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