CN114304159A - Medicament for improving stress resistance of desert oasis protection forest in western arid region - Google Patents
Medicament for improving stress resistance of desert oasis protection forest in western arid region Download PDFInfo
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- CN114304159A CN114304159A CN202111624892.9A CN202111624892A CN114304159A CN 114304159 A CN114304159 A CN 114304159A CN 202111624892 A CN202111624892 A CN 202111624892A CN 114304159 A CN114304159 A CN 114304159A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a medicament for improving stress resistance of a shelter forest in desert oasis in western arid regions, and relates to the technical field of shelter forest construction; the medicament comprises stress-resistant fertilizer and rooting promoter; the stress-resistant fertilizer comprises the following components in parts by weight: 80-100 parts of humic material and 2-6 parts of water-retaining agent; the humic material is obtained by inoculating rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea with excrement and agricultural wastes for fermentation and decomposition; the rooting promoter comprises, by weight, 0.5-2 parts of vitamins, 1-5 parts of amino acids and 1-1.5 parts of auxin. The pesticide can improve the stress resistance of newly planted seedlings in desert oasis protection forests in western arid regions, and improve the survival rate of plants.
Description
Technical Field
The invention relates to the technical field of protection forest construction, in particular to a medicament for improving stress resistance of a protection forest in desert oasis in western arid regions.
Background
A Shelter forest (Shelter forest) is a natural forest and an artificial forest (forest stand refers to the internal structural characteristics of forest trees) which are managed for the purposes of maintaining water and soil, preventing wind and fixing sand, conserving water sources, regulating climate and reducing pollution, and is a forest community which mainly aims at defending natural disasters, maintaining infrastructure, protecting production, improving environment, maintaining ecological balance and the like. However, in desert areas, vegetation is sparse, the earth surface is bare, sand dunes are widely distributed, the natural environment is very severe, artificially newly planted seedlings often cannot withstand drought, the sunlight is strong, the soil moisture is seriously insufficient, the seedlings are easily stressed by drought, are difficult to root or have weak rooting capacity, have weak roots, are easy to fall down by wind, even die, and even if the newly planted seedlings can grow to be plants, the seedlings are easy to have poor nutrition and grow slowly.
Disclosure of Invention
The invention aims to provide a medicament for improving the stress resistance of desert oasis protection forests in western arid regions, and aims to solve the problems of low survival rate, poor growth vigor and easiness in wind fall of the desert oasis protection forests in western arid regions in China. The pesticide can improve the stress resistance of newly planted seedlings in desert oasis protection forests in western arid regions, improve the survival rate of plants and reduce the wind-fall rate.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a medicament for improving the stress resistance of desert oasis protection forests in western arid regions, which comprises stress resistance fertilizers and rooting promoters;
the stress-resistant fertilizer comprises the following components in parts by weight: 80-100 parts of humic material and 2-6 parts of water-retaining agent; the humic material is obtained by inoculating rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea with excrement and agricultural wastes for fermentation and decomposition;
the rooting promoter comprises, by weight, 0.5-2 parts of vitamins, 1-5 parts of amino acids and 1-1.5 parts of auxin.
Further, the water-retaining agent is one or two of polyacrylamide and polyacrylonitrile.
Further, the excrement is one or two of cow dung and horse dung; the agricultural waste is plant straw.
Further, the vitamin is a vitamin complex, and the vitamin complex comprises riboflavin, nicotinamide, cobalamin and calcium D-pantothenate.
Further, the amino acids include glycine, glutamic acid, aspartic acid, histidine and methionine.
Further, the auxin comprises one or more selected from indoleacetic acid, indolepropionic acid, indolebutyric acid and naphthylacetic acid.
The invention also provides a preparation method of the medicament for improving the stress resistance of the desert oasis protection forest in the western arid region,
the preparation method of the stress-resistant fertilizer comprises the following steps:
(1) respectively inoculating rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea to a liquid fermentation culture medium, and respectively culturing to obtain rhizobium zymocyte liquid, bacillus megaterium zymocyte liquid, bacillus subtilis zymocyte liquid, bacillus licheniformis zymocyte liquid, pseudomonas syringae zymocyte liquid and botrytis cinerea zymocyte liquid;
(2) inoculating the rhizobium zymocyte liquid, the bacillus megaterium zymocyte liquid, the bacillus subtilis zymocyte liquid, the bacillus licheniformis zymocyte liquid, the pseudomonas syringae zymocyte liquid and the botrytis cinerea zymocyte liquid obtained in the step (1) into a to-be-decomposed substrate consisting of excrement and agricultural wastes, and fermenting and decomposing to obtain the humic material;
(3) mixing the humic material obtained in the step (2) with a water-retaining agent to obtain the stress-resistant fertilizer;
the preparation method of the rooting promoter comprises the following steps: and dissolving vitamins, amino acid and auxin in water to obtain a mixed solution, namely the rooting promoter.
Further, in the step (2), the inoculation ratio of the rhizobium fermented bacteria liquid, the bacillus megaterium fermented bacteria liquid, the bacillus subtilis fermented bacteria liquid, the bacillus licheniformis fermented bacteria liquid, the pseudomonas syringae fermented bacteria liquid and the botrytis cinerea fermented bacteria liquid is (3-5): (1-3): (1-3): (1-3): (5-10): (5-10).
The invention also provides a method for improving the stress resistance of the desert oasis protection forest in the western arid region, and the medicament for improving the stress resistance of the desert oasis protection forest in the western arid region is applied to the protection forest.
Further, applying the stress-resistant fertilizer to soil for building a shelter forest; and (3) soaking the nursery stock to be built into the protection forest in the rooting promoter and then planting.
Coronatine is a secondary metabolite secreted by part of pathogenic varieties of pseudomonas syringae, is a natural active substance, and has physiological functions of regulating plant growth, enhancing plant stress resistance and the like. Abscisic acid (ABA) can induce the re-synthesis of certain enzymes to increase the stress resistance of plants. However, the coronatine and the abscisic acid are easy to degrade and high in production cost, the pseudomonas syringae soybean pathogenic variety MW123 for producing the coronatine and the botrytis cinerea for producing the abscisic acid are inoculated into excrement and agricultural wastes together with rhizobia, bacillus megaterium, bacillus subtilis and bacillus licheniformis for decomposing and fermenting to obtain the stress-resistant fertilizer which can continuously provide stress-resistant substances such as the coronatine, the abscisic acid and the like for plants, so that the stress resistance of newly-planted seedlings of desert oasis protection forests in western arid regions is enhanced, the survival rate of the plants is improved, and the wind-fall rate is reduced.
The invention discloses the following technical effects:
the stress-resistant fertilizer capable of continuously providing stress-resistant substances such as coronatine, abscisic acid and the like for plants and the rooting promoter capable of promoting the growth of plant root systems are prepared and compounded for planting new seedlings of the shelter forest trees in desert oasis in western arid regions, and the planting after the root dipping treatment of the shelter forest seedlings by using the rooting promoter is beneficial to improving the rooting and root-tying capability of the shelter forest trees in the early stage of planting, preventing the seedlings from being folded backwards and improving the survival rate; the application of the stress-resistant fertilizer to soil before planting is beneficial to the stress resistance of newly-planted seedlings, and the combination of the stress-resistant fertilizer and the newly-planted seedlings can generate a synergistic interaction effect, so that the planting survival rate of the protection forest is remarkably improved, and the wind collapse rate is remarkably reduced.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The "parts" in the present invention are all parts by mass unless otherwise specified.
In the following examples or comparative examples, Pseudomonas syringae was Pseudomonas syringae, A. sojae variety MW123(CGMCC No.2533), Botrytis cinerea was abscisic acid-producing Botrytis cinerea, and Rhizobium was Rhizobium japonicum powder. All the raw materials used in the invention are obtained by direct purchase.
Example 1
(1) Respectively inoculating Rhizobium, Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis and Pseudomonas syringae in LB liquid culture medium (Luria-Bertani liquid culture medium), and respectively culturing to obtain bacterial concentration of 109cfu/mL of zymogen liquid; inoculating Botrytis cinerea into PDA liquid culture medium (Potato Dextrose Agar liquid culture medium), and culturing to obtain a bacterial concentration of 109cfu/mL of the fermentation broth.
(2) And (2) taking 80kg of corn straw and 20kg of cow dung, adding 20kg of water, adding 1000mL of mixed bacterial liquid consisting of the 6 fermentation bacterial liquids (wherein the ratio of the bacterial liquid of rhizobia, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea is 3:3:1:1:10:10), uniformly mixing, controlling the temperature to be 25-40 ℃, and performing aerobic fermentation and decomposition for 15 days to prepare the humic material.
(3) And (3) uniformly mixing 100 parts of the humic material prepared in the step (2) and 2 parts of polyacrylamide to obtain the stress-resistant fertilizer.
(4) Dissolving 5 parts of vitamin (the mass ratio of riboflavin to nicotinamide to cobalamin to D-calcium pantothenate is 1:1:1:1), 10 parts of amino acid (the mass ratio of glycine to glutamic acid to aspartic acid to histidine to methionine is 1:1:1:1) and 10 parts of indoleacetic acid in 1000 parts of water to obtain the rooting promoter.
Example 2
The difference from example 1 is that:
in the step (2), the ratio of the rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea is 5:1:3:3:5: 10;
step (3) is to uniformly mix 80 parts of the humic material prepared in the step (2) and 6 parts of polyacrylonitrile to obtain the stress-resistant fertilizer;
and (4) dissolving 20 parts of vitamin (the mass ratio of riboflavin to nicotinamide to cobalamin to D-calcium pantothenate is 1:1:1:1), 50 parts of amino acid (the mass ratio of glycine to glutamic acid to aspartic acid to histidine to methionine is 1:1:1:1) and 15 parts of indole propionic acid in 1000 parts of water to obtain the rooting promoter.
Example 3
The difference from example 1 is that:
in the step (2), the ratio of the rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea is 4:2:2:2:8: 8;
step (3) is to uniformly mix 90 parts of the humic material prepared in step (2) and 6 parts of polyacrylamide to obtain the stress-resistant fertilizer;
and (4) dissolving 10 parts of vitamin (the mass ratio of riboflavin to nicotinamide to cobalamin to D-calcium pantothenate is 1:1:1:1), 25 parts of amino acid (the mass ratio of glycine to glutamic acid to aspartic acid to histidine to methionine is 1:1:1:1) and 12 parts of indolebutyric acid in 1000 parts of water to obtain the rooting promoter.
Example 4
The difference from example 1 is that: in the step (4), the auxin adopts naphthylacetic acid.
Comparative example 1
The difference from example 3 is that in step (2), the inoculum size of the fermentation broth of Pseudomonas syringae and Botrytis cinerea was not changed, and the inoculum size of the fermentation broth of the other bacteria was not changed.
Comparative example 2
The difference from example 3 is that in step (2), the inoculum size of the fermentation broth of Pseudomonas syringae was not changed, but the inoculum size of the fermentation broth of other bacteria was not changed.
Comparative example 3
The difference from example 3 is that in step (2), the inoculum size of the fermentation broth of Botrytis cinerea is not changed, but the inoculum size of the fermentation broth of other bacteria is not changed.
Comparative example 4
The difference from example 3 is that in step (2), the fermentation broth of pseudomonas syringae and botrytis cinerea is not inoculated, and the inoculum size of the fermentation broth of other bacteria is not changed; and (3) uniformly mixing 90 parts of humic material prepared in the step (2), 6 parts of polyacrylamide, 0.5 part of coronatine and 0.5 part of abscisic acid to obtain the stress-resistant fertilizer.
Effect verification
Selecting a desert oasis land in the western arid region, averagely dividing the desert oasis land into 11 blocks (marked as 1-11 land blocks respectively), and planting Hongsenhuai. Hongsen pagoda is the same batch of annual naked root strong seedlings, the height is about 1.3 m. The plot 11 is used as a control, no medicament is applied, the plots 1 to 8 are respectively applied with the stress-resistant fertilizers and the rooting promoters prepared in the examples 1 to 4 and the comparative examples 1 to 4, the plot 9 is only applied with the stress-resistant fertilizer of the example 3, the plot 10 is only applied with the rooting promoter of the example 3, and the specific application method is as follows:
applying stress-resistant fertilizer to the surface layer of soil and deeply ploughing (the depth is about 40cm) 5 days before planting Hongsen locust tree seedlings, wherein the application amount of the stress-resistant fertilizer is 40 kg/mu; putting the Hongsenhuai seedling root system into a rooting promoter, dipping for 1min, and then planting; after the permanent planting, no auxiliary fixing measures are adopted.
The first day after 6 months, the survival rate of the Hongsen sophora japonica and the wind fall rate are counted, and the results are shown in the table 1, wherein:
the survival rate is the number of surviving plants/the total number of planted plants multiplied by 100 percent;
the air fall rate is the number of plants with an included angle between the trunk and the horizontal line less than 60 degrees/the total number of planted plants multiplied by 100 percent;
TABLE 1
| Land parcel | Examples/comparative examples | Survival rate/% | Wind fall rate/% |
| 1 | Example 1 | 88 | 21 |
| 2 | Example 2 | 91 | 16 |
| 3 | Example 3 | 95 | 11 |
| 4 | Examples4 | 90 | 25 |
| 5 | Comparative example 1 | 69 | 50 |
| 6 | Comparative example 2 | 75 | 41 |
| 7 | Comparative example 3 | 78 | 40 |
| 8 | Comparative example 4 | 82 | 45 |
| 9 | Example 3 stress resistant Fertilizer | 81 | 37 |
| 10 | Example 3 rooting promoter | 83 | 49 |
| 11 | Comparative example | 61 | 58 |
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. A medicament for improving stress resistance of desert oasis protection forest in western arid regions is characterized by comprising stress resistance fertilizer and rooting promoter;
the stress-resistant fertilizer comprises the following components in parts by weight: 80-100 parts of humic material and 2-6 parts of water-retaining agent; the humic material is obtained by inoculating rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea with excrement and agricultural wastes for fermentation and decomposition;
the rooting promoter comprises, by weight, 0.5-2 parts of vitamins, 1-5 parts of amino acids and 1-1.5 parts of auxin.
2. The medicament for improving the stress resistance of the desert oasis shelter forest in the western arid region according to claim 1, wherein the water-retaining agent is one or two of polyacrylamide and polyacrylonitrile.
3. The medicament for improving the stress resistance of the desert oasis shelter forest in the western arid region according to claim 1, wherein the manure is one or two of cow manure and horse manure; the agricultural waste is plant straw.
4. The agent for improving stress resistance of desert shelter forest in western arid region as claimed in claim 1, wherein said vitamin is a vitamin complex comprising riboflavin, niacinamide, cobalamin and calcium D-pantothenate.
5. The agent for improving stress resistance of desert shelter forest in western arid region according to claim 1, wherein the amino acids comprise glycine, glutamic acid, aspartic acid, histidine and methionine.
6. The agent for improving the stress resistance of desert shelter forest in western arid region according to claim 1, wherein the auxin comprises one or more selected from indoleacetic acid, indolepropionic acid, indolebutyric acid, naphthylacetic acid.
7. A preparation method of the medicament for improving the stress resistance of the desert oasis shelter forest in the west arid region according to any one of claims 1 to 6,
the preparation method of the stress-resistant fertilizer comprises the following steps:
(1) respectively inoculating rhizobium, bacillus megaterium, bacillus subtilis, bacillus licheniformis, pseudomonas syringae and botrytis cinerea to a liquid fermentation culture medium, and respectively culturing to obtain rhizobium zymocyte liquid, bacillus megaterium zymocyte liquid, bacillus subtilis zymocyte liquid, bacillus licheniformis zymocyte liquid, pseudomonas syringae zymocyte liquid and botrytis cinerea zymocyte liquid;
(2) inoculating the rhizobium zymocyte liquid, the bacillus megaterium zymocyte liquid, the bacillus subtilis zymocyte liquid, the bacillus licheniformis zymocyte liquid, the pseudomonas syringae zymocyte liquid and the botrytis cinerea zymocyte liquid obtained in the step (1) into a to-be-decomposed substrate consisting of excrement and agricultural wastes, and fermenting and decomposing to obtain the humic material;
(3) mixing the humic material obtained in the step (2) with a water-retaining agent to obtain the stress-resistant fertilizer;
the preparation method of the rooting promoter comprises the following steps: and dissolving vitamins, amino acid and auxin in water to obtain a mixed solution, namely the rooting promoter.
8. The method according to claim 7, wherein in the step (2), the inoculation ratios of the rhizobium fermented bacterial liquid, the bacillus megaterium fermented bacterial liquid, the bacillus subtilis fermented bacterial liquid, the bacillus licheniformis fermented bacterial liquid, the pseudomonas syringae fermented bacterial liquid and the botrytis cinerea fermented bacterial liquid are (3-5): (1-3): (1-3): (1-3): (5-10): (5-10).
9. A method for improving stress resistance of desert oasis shelter forests in western arid regions, which is characterized in that the medicament for improving stress resistance of desert oasis shelter forests in western arid regions according to any one of claims 1 to 6 is applied to the shelter forests.
10. The method according to claim 9, characterized in that the stress-resistant fertilizer is applied in the soil of the shelter forest; and (3) soaking the nursery stock of the protection forest in the rooting promoter and planting.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102326545A (en) * | 2011-06-20 | 2012-01-25 | 中国农业大学 | Drought-resistant agent and application thereof |
| CN102515977A (en) * | 2011-12-27 | 2012-06-27 | 兰州大学 | Water-holding root-promoting halophilic alkaliphilic microorganism root dipping liquid and preparation and application thereof |
| CN102630709A (en) * | 2012-03-29 | 2012-08-15 | 中南林业科技大学 | Stress-resistance and health-care mycorhiza microbial inoculum of Pinus massoniana and application method of stress-resistance and health-care mycorhiza microbial inoculum |
| CN102630708A (en) * | 2012-03-29 | 2012-08-15 | 中南林业科技大学 | Stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and application method of stress-resistance and health-care mycorhiza microbial inoculum |
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- 2021-12-28 CN CN202111624892.9A patent/CN114304159A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326545A (en) * | 2011-06-20 | 2012-01-25 | 中国农业大学 | Drought-resistant agent and application thereof |
| CN102515977A (en) * | 2011-12-27 | 2012-06-27 | 兰州大学 | Water-holding root-promoting halophilic alkaliphilic microorganism root dipping liquid and preparation and application thereof |
| CN102630709A (en) * | 2012-03-29 | 2012-08-15 | 中南林业科技大学 | Stress-resistance and health-care mycorhiza microbial inoculum of Pinus massoniana and application method of stress-resistance and health-care mycorhiza microbial inoculum |
| CN102630708A (en) * | 2012-03-29 | 2012-08-15 | 中南林业科技大学 | Stress-resistance and health-care mycorhiza microbial inoculum of Cunninghamia lanceolata and application method of stress-resistance and health-care mycorhiza microbial inoculum |
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