CN103421724A - Lysobacter antibioticus HY strain and application thereof - Google Patents
Lysobacter antibioticus HY strain and application thereof Download PDFInfo
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Abstract
The invention relates to a lysobacter antibioticus HY strain and application thereof and belongs to the technical field of plant protection. The lysobacter antibioticus HY strain has already been preserved in the China General Microbiological Culture Collection Center on July 11, 2013 and the preservation number is CGMCC No.7912. The konjak rhizosphere bacteria lysobacter antibioticus HY strain is used for preparation of a medicine used for preventing and treating brassicaceous vegetable clubroots, tobacco black shanks and konjak bacterial soft rots in fields. The lysobacter antibioticus HY strain is efficient, non-toxic, safe, free of residue, capable of achieving the disease-control purpose by means of live bacteria, good in plant growth promoting effect, good in comprehensive performance, and suitable for industrialized production, bulk production and application.
Description
Technical field
The present invention relates to the molten bacillus HY of a strain microbiotic and application thereof, platymiscium resist technology field.
Background technology
Crop in cruciferae comprises rape and bulk vegetable, as Chinese cabbage, wild cabbage, Cauliflower, kohlrabi, stem mustard, leaf mustard, radish, Plantula Brassicae chinensis, knurl leaf mustard (hot pickled mustard tube) and special crop Root of Indigowoad and horseradish etc., the annual club root area that occurs reaches more than 2,000 ten thousand mu (inferior), once particularly occur, disease increases the weight of year by year, the cress club root be by the rape plasmodiophora brassicae (
Plasmodiophora brassicaeWoron) infect and cause.The generation of Cruciferae club root, have a strong impact on vegetable crop, when extremely serious, causes underproduction 70%-80%, and No kernels or seeds are gathered, as in a year of scarcity even to allow the vegetable grower, can not be at same plot plantation crop in cruciferae.Due in the last few years, on a large scale, introduce a fine variety at a distance, the club root occurrence scope enlarges for vegetable seed and seedling, and west is from the Huo'erguosi in Xinjiang, the Chayu in Tibet, and to the east of ，Bei Zi Heilungkiang, Taiwan, reaching each provinces and regions, the whole nation, Hainan Island in the south all has distribution.Particularly plastic greenhouse is grown seedlings and has more been accelerated the disease epidemic rate with floating seedlings, causes new lesion can only plant 1 year, and Second Year just can't be planted.Germ can be in soil more than Survival for 10 Years, and rice field-upland field rotation causes germ evenly to be spread, and also can increase the weight of disease.
Tobacco is Solanaceae annual herb plant, is important cash crop.Black shank is a kind of widely distributed, serious worldwide Main Tobacco Diseases of causing harm.The all cultivation tobaccos such as flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, burley tobaccos, Turkish tobaccos of can causing harm.Since finding from Indonesia latter stage in 19th century, this disease runs rampant rapidly, and after the 30's of 20th century, this disease be found everywhere through the world temperate zone, subtropics and De Chanyan district, torrid areas, cause huge financial loss to tobacco production.Black shank, cause a kind of crushing silborne fungal diseases by Phytophthora nicotianae, just infects source for band soil bacteria and invalid body.Can endanger cigarette seedling and land for growing field crops cigarette strain, but general mainly with group's prosperous long front and back morbidity.Each position of cigarette strain all can be injured, and the position that mainly is injured is basal part of stem and the root of strain, can directly infect blade and stem stalk middle and upper part under special conditions, becomes the main soil-borne disease of Yunnan Tobacco Planting Area.Produce at present the measures such as cultivation step, chemical prevention such as above generally adopting crop rotation and control black shank.But due to the restriction of all conditions, uncontrollable this disease.Especially the life-time service of chemical pesticide, can cause the residual of agricultural chemicals and contaminate environment and pathogenic bacteria develop immunity to drugs.
The per nnial herb of Amorphophallus Araeceae Amorphophallus, can extract in a large number the plant of glucomannan, konjaku field planting output is high, good economic benefit is arranged, the commerial growing of all provinces of Southwestern China, and become the Main Economic crop of this area, be also one of this area's effective way of adjusting the structure of agricultural production, peasant programme, development local economy.The konjaku bacterial soft rot is fallen ill all very serious in worldwide, at field growth period and shelf time anniversary, cause harm, the konjaku plant infects soft rot rear blade or bulb deliquescing, blackout is rotted, and foul smelling flavor, flow out from sick section the juice that carries disease germs and infect again new plant, make it morbidity, cause in flakes seedling when serious.Konjak soft rot mainly by the carrot soft rot Pectinatus (
Pectobacterium carotovoraSubsp.
Carotovora, bacterial disease Pcc) caused, the loss caused is generally at 30%-50%, severe patient can reach 80% even total crop failure, has a strong impact on the enthusiasm of taro agriculture plantation konjaku, overslaugh the fast development of southwest local economy.The molten bacillus HY of microbiotic separated from konjaku plant rhizosphere, no matter from indoor antibacterial, or field control has effect preferably.
At present, prevent and treat above-mentioned disease and mainly adopt the agricultural cultivation measure, disease-resistant variety, and Chemical control methods, but prevention effect is all undesirable.
By literature search, there is not yet and utilize the molten bacillus HY of microbiotic to prepare biotechnological formulation, and be applied to control in field crucifer club root, Yan Cao the open report of shin disease and konjak soft rot.
Summary of the invention
The object of the invention is to overcome the deficiency of prior art, the molten bacillus HY of a kind of microbiotic efficient, nontoxic, safe noresidue and application thereof are provided.
The molten bacillus of microbiotic of the present invention (
Lysobacter antibioticus) preservation of HY.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center; Address: No. 3, No. 1, Bei Chen West Road, Chaoyang District, Beijing City institute, Institute of Microorganism, Academia Sinica; Preservation date: on 07 11st, 2013; Preserving number: CGMCC NO.7912.
The molten bacillus of production bacterial strain microbiotic of the present invention
(Lysobacter antibioticus)HY, separate from Fuyuan County, Yunnan Province konjaku vegetables rhizosphere, through morphology, cultivation proterties, conventional Physiology and biochemistry, the full-automatic identification systems of Biolog, measures and 16S rDNA sequencing and phylogeny Epidemiological Analysis thereof, and this produces the molten bacillus of bacterial strain microbiotic
(Lysobacter antibioticus)HY is a new bacterial strain
.
This bacterial strain has following characteristics:
(1) the bacterium colony circle, glossy, smooth, and opaque thin liquid shape produces the chocolate pigment, and initial-stage culture is dark yellow, and the later stage cultivates as brown, does not produce gemma, and sliding phenomenon is arranged.
(2) this bacterial strain antimicrobial spectrum is wide, bacteriostasis is strong, can be in various crop rhizosphere field planting such as Chinese cabbage, tobaccos, the pathogenetic bacterias such as some red palm leaf blight bacterium, konjaku bacterial soft rot bacterium, potato pseudomonas solanacearum, wildfire bacterium, rice leaf spot bacteria, cabbage black rot bacterium are had to stronger restraining effect, especially konjaku bacterial soft rot bacterium.Bacterial strain HY also has restraining effect preferably to fungi, best to sharp Fusariumsp inhibition, is secondly tobacco black shank bacterium, and also very obvious to the fungistatic effect of pseudo-ginseng phytophthora root rot bacterium and dry thread Pyrenomycetes.
(3) there is the living bacterium reason of following physiological and biochemical property: Biolog biochemical character: can utilize dextrin, cyclodextrin, glycogen, polysorbate40, N-acetyl-D-semi-lactosi, N-acetyl-GLUCOSAMINE, ribitol, L-arabinose, cellobiose, D-Fructose, L-fucose, the D-semi-lactosi, gentiobiose, the D-glyconic acid, a-D-glucose, maltose, PEARLITOL 25C, D-glucitol, sucrose, L-Leu, D-Orn, L-Phe, L-PROLINE, L-Histidine, Pidolidone, Serine, glycerine etc.Can not utilize tween 80, phenylethylamine, y-aminobutyric acid, the diacid of going against, D-Ser, p-hydroxybenzoic acid, D-galactonolactone.
The molten bacillus of microbiotic of the present invention (
Lysobacter antibioticus) the HY bacterial strain is for the preparation of control in field crucifer club root preparation, black shank and konjak soft rot, the HY of preservation is inoculated into to (slant culture based formulas: peptone 20g, glycerine 10ml, K on slant medium that is:
2HPO
41.5g, MgSO
4H
2O1.5g, water 1000ml) be positioned in 28 ± 1 ℃ of incubators and cultivate 3 days, can be used as slant strains; Slant strains is received in liquid nutrient medium and is made bacteria suspension, in shaking flask with liquid culture method by the seed amplification culture to fermentor cultivation, fermentative medium formula is as follows: the formula of 1L is peptone 5g, yeast extract paste 1g, MgSO
4H
2O1.5g, K
2HPO
41.5g, glucose 9g, glycerine 10ml, PH6.0-7.0,28 ± 1 ℃ of culture temperature, time 48h); Slack tank sterilization and the sterilization of real tank: the slack tank conditions for sterilization is 0.15Mpa, 45min, and real tank conditions for sterilization is 0.14Mpa, 30min.After the bacterium of having gone out is cooling, inoculation fermentation seed liquor, rotating speed 120rpm, 28 ± 1 ℃ of temperature, ventilation 24m
3/ h.Cultivate 54-72h, in cultivation, after 12h, each 4h sampling check is once.Fermentation secondary fermentation liquid can add diatomite to make solid reagent, Air drying 2 ~ 3 days.
Efficient, nontoxic, safe noresidue that the present invention has, the control of HY bacterial strain viable bacteria is sick, the growth-promoting effect is better, and Comprehensive Traits is better, is easy to the characteristics of suitability for industrialized production, the molten bacillus of its production bacterial strain microbiotic (
Lysobacter antibioticus) HY can be used in the preparation for preparing the control in field crucifer club root, Yan Cao in shin disease and konjak soft rot.
Embodiment:
The molten bacillus of microbiotic of the present invention (
Lysobacter antibioticus) preservation of HY.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center; Address: No. 3, No. 1, Bei Chen West Road, Chaoyang District, Beijing City institute, Institute of Microorganism, Academia Sinica; Preservation date: on 07 11st, 2013; Preserving number: CGMCC NO.7912.
The molten bacillus of microbiotic of the present invention (
Lysobacter antibioticus) in the preparation of HY bacterial strain for the preparation of control in field crucifer club root, black shank and konjak soft rot, that is: the HY of preservation is inoculated into to (the slant culture based formulas of 1L: peptone 20g, glycerine 10ml, K on slant medium
2HPO
41.5g, MgSO
4H
2O1.5g, water 1000ml) be positioned in 28 ± 1 ℃ of incubators and cultivate 3 days, can be used as slant strains; Slant strains is received in liquid nutrient medium and made bacteria suspension, and in shaking flask, with liquid culture method, by the seed amplification culture, to the amount of the required seed liquor of fermentor cultivation, (formula of liquid culture based formulas: 1L is peptone 5g, yeast extract paste 1g, MgSO
4H
2O1.5g, K
2HPO
41.5g, glucose 9g, glycerine 10ml, PH6.0-7.0,28 ± 1 ℃ of culture temperature, time 48h); Slack tank sterilization and the sterilization of real tank: the slack tank conditions for sterilization is 0.15Mpa, 45min, and real tank conditions for sterilization is 0.14Mpa, 30min.After the bacterium of having gone out is cooling, inoculation fermentation seed liquor, rotating speed 120rpm, 28 ± 1 ℃ of temperature, ventilation 24m
3/ h.Cultivate 54-72h, in cultivation, after 12h, each 4h sampling check is once.Fermentation secondary fermentation liquid can add diatomite to make solid reagent, Air drying 2 ~ 3 days.
The biotechnological formulation preparation prepared with production bacterial strain of the present invention, use by the mode of filling with root.In greenhouse and Chuxiong Prefecture Lufeng County field, Yunnan Province, carried out efficiency test, proved this biotechnological formulation preventive effect significantly, stable, can effectively control the cress club root, the prevention effect of cress club root is reached to 58%-77%.
By in community, Deng Di field, Malong County, Yuxi and greenhouse, having carried out efficiency test, biocontrol fungicide HY can the effectively preventing black shank.
Greenhouse and land for growing field crops prevent and treat process, find that bacterial strain of the present invention is to the konjak soft rot prevention effect also very remarkable in greenhouse and large Tanaka, reach more than 75%.
Below with embodiment, be described in further detail the present invention, but content of the present invention is not limited to this.
Embodiment mono-: the test of pesticide effectiveness of biocontrol microorganisms HY preparation to the Chinese cabbage club root
(1) the in vitro bacteriostatic test of biocontrol strain
1. for the examination material:
1.1 strains tested:The molten bacillus of microbiotic (
Lysobacter antibioticus) HY.
For the examination pathogenic bacteria
Plant pathogenetic bacteria (konjaku bacterial soft rot bacterium, red palm leaf blight bacterium, rice leaf spot bacteria, xanthomonas oryzae pv. oryzicola, Chinese cabbage soft rot bacteria, cabbage black rot bacterium, potato pseudomonas solanacearum, wildfire bacterium), pathogenic fungi (pseudo-ginseng phytophthora, sharp Fusariumsp, dry thread Pyrenomycetes, black shank), provide by Yunnan Prov Agriculture University's species diversity and disease control Bacterial Laboratory, national project center.
For having a try agent and substratum bacterium
NA substratum (peptone 5g, beef extract 3g, yeast extract 1g, sucrose 10g, agar 17g, water 1000mL, pH7.0); PDA substratum (potato 200g, agar 15g, glucose 15g, water 1000mL).
Test method
Antagonistic effect adopts dull and stereotyped face-off
1) preparation of bacteria suspension: the molten bacillus of the microbiotic obtained and pathogenetic bacteria are accessed respectively in the triangular flask of the NA liquid nutrient medium that 50 ml are housed, be placed on 28 oC, 160 r/min shaking tables and cultivate 48 h.Bacteria suspension is suitably diluted with sterilized water, after shaking evenly, with 600 nm wavelength, 1 cm cuvette, measure the OD value.Make blank with aseptic NA substratum during mensuration.With sterilized water dilution make bacteria suspension for OD value 0.5, stand-by.
2) to the mensuration of pathogenetic bacteria bacteriostatic action: get upper step gained pathogenetic bacteria bacteria suspension 200 ul and add in culture dish, the NA substratum that is cooled to 45 oC left and right is poured in the culture dish that added the pathogenetic bacteria bacteria suspension, mix.After fully solidifying, place the Oxford cup in media surface, get step gained HY bacteria suspension 200 μ l and add the Oxford cup, cultivate 2-4 d in 28 oC.Observation, log.
3) to the mensuration of pathogenic fungi bacteriostatic action: at the dull and stereotyped center inoculation of PDA phytopathogen bacterium dish (diameter 5 mm), 2 cm places, both sides inoculate the HY bacterial strains.Put in 28 ℃ of incubators and cultivate, observe antibacterial situation.
The fungistatic effect of table 1 biocontrol microorganisms HY bacterial strain to plant pathogenetic bacteria
Embodiment bis-: the greenhouse Controlling effect of biocontrol microorganisms HY preparation to the Chinese cabbage club root
1, experiment material and method
1.1, for the examination microbial inoculum: biocontrol microorganisms HY liquor
1.2, for studying thing: Chinese cabbage
1.3, controlling object: the Chinese cabbage club root
1.4. experiment place: plant protection institute of Yunnan Province agriculture university warmhouse booth, the high sense of potted plant growth club root Chinese cabbage cultivar 83-1,3 repetitions are established in every processing.
The preparation of pathogenic bacteria and biocontrol fungicide:
1.5.1 the preparation of inoculum
Every part of germ is got bright quality 15 g homogenate, configures 100 mL bacterium liquid, and utilizing colony counting method to make bacterium liquid spore concentration is 12 * 10
7Individual/mL, join bacterium liquid in the air-dry turfy soil of 300 g, stirs, and faling apart that its water content is agglomerating to hold, tactile is advisable, and more than sealing 48 h, the germ spore concentration is 4 * 10
7Individual/g.
Inoculation method
Compost is pressed the peat composed of rotten mosses: vermiculite: perlite=2
V: 1
V: 1
VPreparation, the nutrition pot of packing into (specification is 10cm * 10 cm), water Shui， center, vola and insert the spherical cavity of dark 3 cm, diameter 2 cm with solid bar, inserts germ soil.Every part of germ soil packing 10 alms bowls.Every alms bowl is broadcast 4 seeds, all broadcasts on germ soil, and every part of bacterium soil is kept a full stand of seedings more than 20 strains.After planting seedbed ground temperature is controlled at 25 ℃, and soil humidity remains on 90 %, and the pH value is controlled at 6～7, for good condition is built in the morbidity of germ.
Biocontrol microorganisms HY liquid preparation different concns diluent
By biocontrol fungicide HY liquid preparation, be diluted with water to the bacterium liquid containing different concns, adopt dilution to be coated with the concentration that flat band method is measured each diluent, with watering the root method, every strain Chinese cabbage is watered 30ml at seeding time, and water once after 7 days at interval again, then, after 15 days, water for the third time.
State of an illness investigation
After inoculation, 45d institutes an inquiry, severity Scaling standard (1):
0 grade: root is without tumour;
1 grade: lateral root has little tumour;
3 grades: the main root enlargement, its diameter is less than 2 times of basal part of stem;
5 grades: the main root enlargement, its diameter is 2-3 times of basal part of stem;
7 grades: the main root enlargement, its diameter is 3-4 times of basal part of stem;
9 grades: the main root enlargement, its diameter be basal part of stem more than 4 times or the root of enlargement blackening appears.
Disease index=[Σ (diseased plant numbers at different levels * relative level numerical value)/highest level * investigate total strain number] * 100
Sickness rate=(morbidity strain number/investigate total strain number) * 100
Relative control effect=(contrast disease index-processing disease index) * 100/ contrast disease index
Test results and analysis
Table 2 biocontrol fungicide HY control Chinese cabbage club root effect
| Strain name | Bacterial concentration (CFU/ml) | Disease index | Relative control effect (%) |
| HY | 10 8 | 25.42 | 70.09 |
| HY | 10 7 | 19.13 | 77.49 |
| HY | 10 6 | 23.63 | 72.20 |
| Cyazofamid | Dilute 2000 times | 28.00 | 67.06 |
| CK | Clear water | 85.00 | — |
As can be known from Table 2, biocontrol strain HY liquid preparation has good prevention effect to the Chinese cabbage club root, in biocontrol fungicide concentration (bacteria containing amount), is 10
6During CFU/ml, to the prevention effect of cabbage clubroot, be 72.20%, higher than 67.06% preventive effect of 2000 times of processing of chemical pesticide cyazofamid.And be 10 in biocontrol fungicide concentration (bacteria containing amount)
7During CFU/ml, preventive effect is apparently higher than the agricultural chemicals cyazofamid.
Embodiment tri-: biocontrol microorganisms HY preparation control Chinese cabbage club root field control effectiveness test
The sowing Chinese cabbage seeds, variety adoption becomes assorted No. 5, and for examination material random alignment, each material is planted 15 row, and every row 6 young plants arrange three repetitions.Test site is carried out in county magistrate low-lying area, Lufeng, Yunnan Province.
Biocontrol fungicide HY solid preparation, respectively 100 times, 200 times of dilute with waters and 500 times of liquid, adopt the root method of watering, and after planting 7 days, 14 days and 21 days, the bacteria suspension of the biocontrol strain HY for preparing filled with to root.Every strain Chinese cabbage seedling is filled with bacterium 200mL.
2000 times of diluents of contrast sterilant cyazofamid, adopt above-mentioned same method to water root, and spraying times is 3 times, and every strain Chinese cabbage seedling is filled with bacterium 200ml.
Blank: use clear water, adopt above-mentioned same method to water root, application times is 3 times, and every strain Chinese cabbage seedling is filled with clear water 200ml.
5 processing are established in test, repeat Gong15Ge community, 30 square metres of community areas, random alignment for 3 times.
In test, in the disease occurrence in peak period, sampled, 50 strains of at every turn sampling, investigate and record physical signs and the morbidity grades such as each kind plant height, leaf area, plant fresh weight, statistics club root incidence and disease index, analytical results.
State of an illness investigation is undertaken by following standard.
After inoculation, 45d institutes an inquiry, and the severity Scaling standard is as follows:
0 grade: root is without tumour;
1 grade: lateral root has little tumour;
3 grades: the main root enlargement, its diameter is less than 2 times of basal part of stem;
5 grades: the main root enlargement, its diameter is 2-3 times of basal part of stem;
7 grades: the main root enlargement, its diameter is 3-4 times of basal part of stem;
9 grades: the main root enlargement, its diameter be basal part of stem more than 4 times or the root of enlargement blackening appears.
Disease index=[Σ (diseased plant numbers at different levels * relative level numerical value)/highest level * investigate total strain number] * 100
Sickness rate=(morbidity strain number/investigate total strain number) * 100
Relative control effect=(contrast disease index-processing disease index) * 100/ contrast disease index
Field test results is as follows:
Pass through field efficiency test, as seen from Table 3: using biocontrol microorganisms HY biotechnological formulation has good prevention effect to the Chinese cabbage club root, wherein the effect of 100 times of biotechnological formulation dilutions is best, still reach 75.16% in harvesting time control, the prevention effect of diluting 200 times and 500 times reaches respectively 62.63 and 57.67%.With produce in 66.31% the comparing of processing of 2000 times of the commonly used cyazofamids of preventing and treating the club root disease, sickness rate and the disease index of using 100 times of each processing of biological agent H Y all decrease, and have shown that this bacterium has potential development prospect.
Table 3 biocontrol fungicide HY control Chinese cabbage club root effect (the hollow test point of Lufeng county magistrate)
| Process | Average diseased plant rate (%) | Average disease refers to | Prevention effect (%) |
| CK | 80 | 46.3 | — |
| Dilute 100 times | 34 | 11.5 | 75.16 |
| Dilute 200 times | 37 | 17.3 | 62.63 |
| Dilute 500 times | 45 | 19.6 | 57.67 |
| 2000 times of cyazofamids | 37 | 15.6 | 66.31 |
Embodiment tetra-: biocontrol microorganisms HY preparation is to the sick test of black shank field control
1, experiment material and method
1.1, reagent agent: biocontrol microorganisms HY wettable powder
1.2, for studying thing: tobacco, the large gold dollar of safflower
1.3, controlling object: black shank
1.4. experiment place: Malong County, beautiful Qujing, the Yunnan Province horse balck shank serious vega of falling ill that crosses the river, middle fertility, each processes 30 strains, and 3 repetitions are established in every processing, random alignment, the Gong12Ge community, surrounding is established the protection row, presses high yield, the standardized management of High Quality Tobacco cultivation step.
Test method:
100 times of biocontrol fungicide HY dilute with waters are filled with root after tobacco transplant, and group's phase is filled with root once again, use altogether 2 every strain tobaccos and fill with bacterium 200mL.
500 times of contrast fungicide metalaxyl MnZn dilutions, rich ground element dilutes 1000 times and adopts above-mentioned same method to water root, and spraying times is 2 times, and every strain Chinese cabbage seedling is filled with bacterium 200ml.
Blank: use clear water, adopt above-mentioned same method to water root, application times is 3 times, and every strain tobacco is filled with clear water 200ml.
In test, in the disease occurrence in peak period, investigated, 20 strains are investigated in each community, investigate and record physical signs and the morbidity grades such as each kind plant height, leaf area, plant fresh weight, statistics club root incidence and disease index, analytical results.
State of an illness investigation is undertaken by following standard
0 grade: complete stool is anosis;
1 grade: stem's scab is no more than 1/2 of stem girth, or half scab occurs with the slight wilting or bottom minority blade of lower blade;
2 grades: stem's scab surpasses 1/2 of stem girth, or blade more than half is slightly wilting;
3 grades: stem's scab is around stem girth, or 2/3 is wilting with blade;
4 grades: the whole blades of diseased plant are wilting or withered.
Sickness rate=(morbidity strain number/investigate total strain number) * 100
Disease index=[Σ (diseased plant numbers at different levels * relative level numerical value)/highest level * investigate total strain number] * 100
Relative control effect=(contrast disease index-processing disease index) * 100/ contrast disease index
2. test results and analysis
From table 4, know, biocontrol microorganisms HY preparation adopts the effect of watering the processing of root method best, and the field sickness rate that biocontrol microorganisms and chemicals treatment are crossed and disease index have obvious reduction with respect to contrast, and the difference of disease index reaches significance level.Prevention effect in harvesting time investigation to balck shank, biocontrol fungicide HY is up to 70.45%, apparently higher than 61.36% of the processing of 1000 times of 54.55% and rich ground elements of 500 times of the pesticide metalaxyl MnZns of preventing and treating balck shank.Illustrate that biocontrol fungicide HY has good prevention effect to black shank.
The field control effect (test point, Malong County) of table 4 biocontrol microorganisms HY preparation control black shank
| Process | Sickness rate (%) | Disease index | Relative control effect (%) |
| HY100 doubly | 30 | 16.25 | 70.45 |
| 500 times of metalaxyl-mn-zns | 45 | 25 | 54.55 |
| 1000 times of rich ground elements | 35 | 21.25 | 61.36 |
| CK | 85 | 55 |
Embodiment five: the prevention effect of biocontrol fungicide HY to konjak soft rot
1.1, for the examination biocontrol fungicide: biocontrol fungicide HY liquor
1.2, for studying thing: the natural resources elephant-foot yam
1.3, controlling object: the konjaku bacterial soft rot
1.4. test site: konjaku planting base, Fuyuan County, Yunnan Province, middle fertility, community is furrow 4 row districts, 30 square metres of areas, 3 repetitions are established in every processing, random alignment, totally 9 communities, surrounding is established the protection row, presses high yield, High-quality Cultivation measure standardized management.
Biocontrol fungicide processing, clear water contrast, 3 processing of agricultural chemicals contrast are established in test.The method of dispenser adopts root-pouring method, and every konjaku root waters liquid 200 mL, executes medicine for the first time before their early stage, and be one week interval, executes medicine 3 times, last dispenser 15 d " Invest, Then Investigate " data.
Investigation method and disease scale
According to the people's such as Zhao Jiajun research, in conjunction with practical situation, for reflecting more accurately disease, serious situation occurs, we by the revision of konjak soft rot grade scale are:
According to the morbidity severity, konjak soft rot is divided into to the 0-4 level:
0 grade: plant strain growth is normal, and blade is without the non-physiologic jaundice, without the water soaking mode scab;
The l level: blade (or petiole) side has small-sized water soaking mode scab, and lesion area (or long) accounts for below 10% of total leaf area (petiole total length); Perhaps the slight yellow area of blade is less than 10%;
2 grades: water soaking mode scab or the yellow area of blade (or petiole) account for total leaf area 10%-40%, and basal part of stem or stem have the water soaking mode scab, but plant is upright.
3 grades: water soaking mode scab or the yellow area of blade (or petiole) account for total leaf area 40%-90%, and basal part of stem has obvious soft rotten symptom or the whole soft corruption of stem one lateral lobe handle, and plant is crooked.
4 grades: the water soaking mode scab of blade (or petiole) or yellow area account for total leaf area more than 90% or full leaf is withered and yellow, the plant lodging is rotted.
The field efficiency test result proves (in Table 5), biocontrol fungicide HY compared with the control, be that average attack rate, average disease index all decrease, particularly to the preventive effect of konjaku bacterial soft rot, more than 75%, preventive effect obviously exceeds much than agricultural chemicals MBAMT biocontrol microorganisms stoste.Illustrate that biocontrol fungicide HY has good prevention effect to the konjaku bacterial soft rot.
The preventive effect (Fuyuan County konjaku planting base) of table 5 biocontrol fungicide HY to the konjaku bacterial soft rot
| Processing mode | Sickness rate (%) | Disease index | Relative control effect (%) |
| Biocontrol fungicide HY | 21.3 | 11.1 | 75.1 |
| MBAMT | 48.9 | 23.1 | 48.2 |
| CK | 62.6 | 44.6 |
Claims (2)
1. the molten bacillus HY of a strain microbiotic, be characterised in that producing the molten bacillus of bacterial strain microbiotic (Lysobacter antibioticus) HY has been deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC NO.7912 on 07 11st, 2013.
2. the molten bacillus HY of a strain microbiotic claimed in claim 1 is preparing the control in field crucifer club root, the application in black shank and konjak soft rot soil-borne disease preparation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667129A (en) * | 2013-12-06 | 2014-03-26 | 云南农业大学 | Lysobacter antibioticus 06-4 and application thereof |
| CN103667136A (en) * | 2013-12-10 | 2014-03-26 | 南京农业大学 | Separation and identification of lysobacter antibioticus OH13 capable of antagonizing plant pathogenic bacteria |
| CN106011222A (en) * | 2016-05-23 | 2016-10-12 | 中国农业科学院郑州果树研究所 | Agrobacterium tumefaciens resistance identification method of peach trees and sibling species plants thereof |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667129A (en) * | 2013-12-06 | 2014-03-26 | 云南农业大学 | Lysobacter antibioticus 06-4 and application thereof |
| CN103667136A (en) * | 2013-12-10 | 2014-03-26 | 南京农业大学 | Separation and identification of lysobacter antibioticus OH13 capable of antagonizing plant pathogenic bacteria |
| CN106011222A (en) * | 2016-05-23 | 2016-10-12 | 中国农业科学院郑州果树研究所 | Agrobacterium tumefaciens resistance identification method of peach trees and sibling species plants thereof |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
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