CN106942283A - A kind of tobacco seed sprouts strengthening agent and its preparation, application method - Google Patents
A kind of tobacco seed sprouts strengthening agent and its preparation, application method Download PDFInfo
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- CN106942283A CN106942283A CN201710166216.9A CN201710166216A CN106942283A CN 106942283 A CN106942283 A CN 106942283A CN 201710166216 A CN201710166216 A CN 201710166216A CN 106942283 A CN106942283 A CN 106942283A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention belongs to tobacco cultivation technical field, and in particular to a kind of tobacco seed sprouts strengthening agent, and further discloses its preparation and application method.It is unique that tobacco seed of the present invention sprouts strong sprout agent prescription, using quick lime, bacillus subtilis microbial inoculum, plant ash, ginkgo biloba extract as raw material, under the premise of existing seedling-raising technique measure is not changed, you can improve the cold-resistant resistance of seed and the physiological effect of improving growth.
Description
Technical field
The invention belongs to tobacco cultivation technical field, and in particular to a kind of tobacco seed sprouts strengthening agent, and further public
It is opened to prepare and application method.
Background technology
Tobacco is the happiness temperature crop for originating in America, is also a kind of important industrial crops in current China's agricultural production.
The optimum growth temperature of tobacco is 25-28 DEG C, and then quality is more inferior for ripe tobacco leaf below 16-17 DEG C of temperature, and is less than
It can then be stopped growing at 10-13 DEG C, seedling will be dead under as little as 1-2 DEG C low temperature.
Existing tobacco planting mainly uses greenhouse floating seedlings technology, and floating seedlings are a new method for culturing seedlings, begins
In late 1980s, American Tobacco carries out nursery using floating seedlings method.Floating seedlings technology is to will be equipped with lightweight nursery
The foam hole tray of matrix is floated on the water surface, and seed is sowed in matrix, and rice shoot takes root growth in seedling medium, and can be from base
The method for culturing seedlings of moisture and nutrient is absorbed in matter and water bed.Compared with traditional seedling method, it, which has, can reduce transplanting recruitment, save
Nursery land used, be easy to tobacco seedlings management, be conducive to nurturing staff, improve planting percent the advantages of.
The main provinces and regions on the south the Changjiang river in China's tobacco planting region, because tobacco sowing is main in annual winter, early spring
Chilling damage turns into south China cigarette district common problem.During nursery, in atmospheric temperature and seedling raising greenhouse
Water temperature in nursery pond is relatively low, illumination is not enough, under such a low temperature and less sunshine environment, often leads to Tobacco Seed Germination and emerges
The problems such as difficult, slow, irregular, seedling of emerging of emerging is weak, seedling is easy to be susceptible, also causes emergence rate, planting percent and strong sprout rate low, seriously
The raising of seedling quality is influenceed, and transplants normal growth and the development of later plant.
Low temperature, few shine adversely affect to nursery during nursery is solved in current tobacco leaf production, and major measure is still to pass through
Covering with ground sheeting heats and increased the measures such as the illumination in greenhouse；And control the measure of disease during nursery to remain using chemistry
Medicament.Calcium chloride, polyethylene glycol, gibberellin (GA3), diformazan are commonly used in the winter resistance of crop, production in order to improve
The reagents such as base sulfoxide, salicylic acid, proline, abscisic acid (ABA), uniconazole P, calcium gluconae, chitosan, its major physiological work(
It can be exactly to increase cell liquid concentration, cell leakage be reduced, to improve the adverse effect caused to low temperature environment.But these cold-resistants
Reagent function is more single, and under low temperature environment, seed sprouts later seedling to the harmful microorganism in environment and germ
Resistance it is very weak, easily introduced disease.And prior art other find to take chemistry after disease hazard in seedling raising process
The method of chemical control, there is also curing the symptoms, not the disease, high cost, the defect such as unfriendly to environment.
The content of the invention
Therefore, the technical problems to be solved by the invention are to provide a kind of tobacco seed to sprout strengthening agent, it is existing to solve
There is the problem of causing tobacco seed emergence rate, planting percent, low strong sprout rate because of low temperature and less sunshine in technology.
In order to solve the above technical problems, a kind of tobacco seed of the present invention sprouts strong sprout composition, including following weight
Measure the component of part：Quick lime 80-150 parts by weight, bacillus subtilis microbial inoculum 0.5-1.5 parts by weight, plant ash 80-200 parts by weight,
Ginkgo biloba extract 5-15 parts by weight.
It is preferred that, described tobacco seed sprouts strong sprout composition, includes the component of following parts by weight：The weight of quick lime 100
Measure part, the parts by weight of bacillus subtilis microbial inoculum 1, the parts by weight of plant ash 150, the parts by weight of ginkgo biloba extract 10.
The bacillus subtilis microbial inoculum is the bacillus subtilis microbial inoculum WP that bacteria containing amount is higher than 10,000,000,000 living spores/g.
The ginkgo biloba extract is ginkgo leaf and/or ginkgo nut pericarp extract.
The ginkgo biloba extract is extracted as follows：Dry ginkgo leaf and/or ginkgo nut pericarp is taken to crush, according to
1：3.5-5.5 mass ratio and 70-80wt% ethanol solution are mixed, and extract 4-5h at a temperature of 40-45 DEG C, and by extract solution
The paste that moisture content is less than 10% is concentrated into, is produced.
Sprouting strong sprout composition the invention also discloses described tobacco seed is used to prepare tobacco seed sprouting strengthening agent
Purposes.
Strengthening agent is sprouted the invention also discloses a kind of tobacco seed, is that strong sprout composition is sprouted by described tobacco seed
Add water the aqueous solution being made.
The tobacco seed sprouts strong sprout composition and accounts for the mass concentration of the aqueous solution for 0.5%-2.5wt%, and excellent
Select 1.5wt%.
The method that described tobacco seed sprouts strengthening agent is prepared the invention also discloses a kind of, including is measured according to selected
The step of quick lime, bacillus subtilis microbial inoculum, plant ash, ginkgo biloba extract are mixed, and the obtained selected quality that is dissolved in water
The aqueous solution of concentration, is produced.
Institute is placed in the invention also discloses the application method that described tobacco seed sprouts strengthening agent, including by tobacco seed
State tobacco seed and sprout the step of being soaked 15-45 minutes in strengthening agent, dry water.
Tobacco seed of the present invention sprouts strong sprout agent prescription uniqueness, with quick lime, bacillus subtilis microbial inoculum, vegetation
Ash, ginkgo biloba extract are raw material, under the premise of existing seedling-raising technique measure is not changed, you can improve the cold-resistant resistance of seed
And the physiological effect of improving growth.Quick lime produces substantial amounts of Ca after being dissolved in water2+, Ca2+It is intracellular the second important letter
Make, its signal transduction mediated can alleviate injury of the environment stress to plant；Contain substantial amounts of K in plant ash+, it is certain density
K+Effectively maintain the activity of some intracellular enzymes, Ca2+And K+The metabolism of endocellular sugar and protein, K are participated in jointly+In plant
Interior function is mainly more than 60 kinds of enzyme of activation, and participation includes Hill reaction, photosynthetic electron transfer, photosynthetic phosphorylation, dioxy
Change physiological function in terms of the fixation and assimilation and the transport of photosynthate of carbon, by adjusting auxin (heteroauxin) and red
Mycin influences cell elongation, and the turgescence control stomatal movement of regulation Stomacal guard cell controls rising dehydration, statocyte
The anionic charge of macromolecular or the transferable anionic charge in vacuole, xylem and bast in structure, are protected simultaneously
Hold these position pH value, promote absorption and transhipment of the root to nitrate, promote position transport that amino acid is synthesized to protein and
Protein is synthesized, the negative electrical charge in balance acidic amino acid, makes protein structure stable；Bacillus subtilis thalli growth process
The subtilin of middle generation, polymyxins, nystatin, gramicidins isoreactivity material, these active materials to pathogenic bacteria or
The conditioned pathogen of autogenous infection has an obvious inhibitory action, improves immunity, and bacillus subtilis thalline itself synthesis α-
The enzymes such as amylase, protease, lipase, cellulase, and can a variety of B races dimensions such as synthetic vitamin B1, B2, B6, nicotinic acid
Raw element, improves the resistance of Seed Germination；Using winter resistance active material in ginkgo leaf or ginkgo nut pericarp extract, lead to
Cross rice shoot after neutron absorption and budding to absorb, strengthening the cold-resistant anti-adversity of seed, it is ensured that rice shoot normal growth is sent out
Educate, nurturing staff.
The processing for the mixed aqueous solution that scheme of the present invention passes through above-mentioned substance, the cold-resistant that can improve tobacco seed resists
Against performance, promotion germination improves emergence rate, planting percent and strong sprout rate with sprouting, and seedling is disease-resistant after raising seed sprouting
Performance, lowers the tobacco seedlings incidence of disease.But single Ca2+And K+By increasing the concentration of cell liquid, cell leakage is reduced, only
To improve the cold performance of seed and seedling；Bacillus subtilis also can only only improve resistance of the seedling to germ, if by Ca2+
And K+And bacillus subtilis is organically combined together, and Seed Treatment is carried out prior to seeding, can both be improved in Seed Germination
Winter resistance, can improve germination and emerge later seedling to harmful microbe resistance in environment, mitigate the harm of germ again,
The robust growth development of tobacco seedlings is ensured, so as to the dual purpose for reaching cold-resistant growth promotion, mitigating disease hazard.
It is demonstrated experimentally that tobacco seed of the present invention sprouts strengthening agent, potentiality of seed 3.1%- can be effectively improved
7.2%th, germination percentage 3.6%-7.2%, germination index 6.1-17.9, planting percent 4.2%-8.7%, strong sprout rate 2.9%-9.5%,
Seedling Ratio of dry to fresh matter of leaves 0.30-1.25 is reduced, cigarette strain diseased plant rate 2.1%-5.86% is reduced, shortens into seedling stage 5-8d.Cold-resistant is improved to resist
The physiological effect of inverse and improving growth is notable.
Tobacco seed of the present invention sprouts strengthening agent, and raw material sources are extensive and cheap：Quick lime is in China
There is production southern area, is fired after the limestone crushing being widely present by nature, is that building trade is commonly used
One of material, in the market is easy to buying and arrived；Bacillus subtilis commodity microbial inoculum has multiple manufacturing enterprises to have supply at home；
Plant ash is obtained in southern china rural area peasant family such as cooks at the life by burn dry branches and fallen leaves and weeds etc.；South plantation
Substantial amounts of ginkgo, can suitably pluck blade and smash to pieces, and it is standby to extract juice refrigeration, can also remove pericarp in ginkgo nut harvesting
When, pericarp squeeze juice is refrigerated standby.
Tobacco seed of the present invention sprouts strengthening agent, and compound method is simple and easy to do, as long as by quick lime, withered grass gemma
Bacillus, plant ash and ginkgo leaf or ginkgo nut pericarp extract, are mixed according to certain mass ratio, be placed in plastics or clay burns
In the container of system, or in cement pit, it is subject to the clean water of certain mass, fully dissolving can be just configured to.
Embodiment
Embodiment 1
Tobacco seed described in the present embodiment sprouts strong sprout composition, includes the component of following parts by weight：Quick lime 80g,
Bacillus subtilis microbial inoculum (10,000,000,000 living spores/g, WP) 1.5g, plant ash 80g, ginkgo biloba extract 15g.
The ginkgo biloba extract is made as follows：First ginkgo leaf is dried to biodiversity content at 60 DEG C
Less than 13%, it is subsequently cooled to crush after room temperature, crosses 100-200 mesh sieves；The ginkgo leaf of crushing is pressed with 80wt% ethanol solutions
According to mass ratio 1:3.5 ratio, the mechanical shaking extraction 4-5h in the case where temperature is 40-45 DEG C of environment, extract solution is put into Rotary Evaporators
Paste of the moisture content below 10% is concentrated into, is produced.
Take above-mentioned tobacco seed to sprout strong sprout composition to mix, take 10g gained mixtures to add clean water dissolves to be configured to matter
The aqueous solution that concentration is 0.5wt% is measured, described tobacco seed is produced and sprouts strengthening agent.
Embodiment 2
Tobacco seed described in the present embodiment sprouts strong sprout composition, includes the component of following parts by weight：Quick lime 150g,
Bacillus subtilis microbial inoculum (10,000,000,000 living spores/g, WP) 0.5g, plant ash 200g, ginkgo biloba extract 5g.
The ginkgo biloba extract is made as follows：First ginkgo nut pericarp is dried to biodiversity content at 80 DEG C
Less than 13%, it is subsequently cooled to crush after room temperature, crosses 100-200 mesh sieves；The ginkgo nut pericarp and 70wt% ethanol of crushing is molten
Liquid is according to mass ratio 1：5.5 ratio, the mechanical shaking extraction 4-5h in the case where temperature is 40-45 DEG C of environment, extract solution is put into rotary evaporation
Paste of the moisture content below 10% is concentrated into instrument, is produced.
Take above-mentioned tobacco seed to sprout strong sprout composition to mix, take 10g gained mixtures to add clean water dissolves to be configured to matter
The aqueous solution that concentration is 2.5wt% is measured, described tobacco seed is produced and sprouts strengthening agent.
Embodiment 3
Tobacco seed described in the present embodiment sprouts strong sprout composition, includes the component of following parts by weight：Quick lime 100g,
Bacillus subtilis microbial inoculum (10,000,000,000 living spores/g, WP) 1g, plant ash 150g, ginkgo biloba extract 10g.
The ginkgo biloba extract is made as follows：First ginkgo nut pericarp is dried to biodiversity content at 70 DEG C
Less than 13%, be subsequently cooled to crush after room temperature, cross 100-200 mesh sieves, by the ginkgo leaf of crushing or ginkgo nut pericarp with
75wt% ethanol solutions are according to mass ratio 1：4.5 ratio, mechanical shaking extraction 4-5h, extract solution in the case where temperature is 40-45 DEG C of environment
The paste that moisture content is concentrated into Rotary Evaporators below 10% is put into, is produced.
Take above-mentioned tobacco seed to sprout strong sprout composition to mix, take 10g gained mixtures to add clean water dissolves to be configured to matter
The aqueous solution that concentration is 1.5wt% is measured, described tobacco seed is produced and sprouts strengthening agent.
Embodiment 4
Tobacco seed described in the present embodiment sprouts strong sprout composition, includes the component of following parts by weight：Quick lime 90g,
Bacillus subtilis microbial inoculum (10,000,000,000 living spores/g, WP) 1.2g, plant ash 120g, ginkgo biloba extract 12g.The ginkgo biloba extract
It is made according to method in embodiment 3.
Take above-mentioned tobacco seed to sprout strong sprout composition to mix, take 10g gained mixtures to add clean water dissolves to be configured to matter
The aqueous solution that concentration is 1wt% is measured, described tobacco seed is produced and sprouts strengthening agent.
Embodiment 5
Tobacco seed described in the present embodiment sprouts strong sprout composition, includes the component of following parts by weight：Quick lime 120g,
Bacillus subtilis microbial inoculum (10,000,000,000 living spores/g, WP) 0.8g, plant ash 180g, ginkgo biloba extract 8g.The ginkgo biloba extract is pressed
It is made according to method in embodiment 3.
Take above-mentioned tobacco seed to sprout strong sprout composition to mix, take 10g gained mixtures to add clean water dissolves to be configured to matter
The aqueous solution that concentration is 2wt% is measured, described tobacco seed is produced and sprouts strengthening agent.
Experimental example
Experimental example 1
It is cloud and mist 87 for planting experimentally son, sows place in the floating seedlings greenhouse that Hunan Province Guiyang County outskirts of a town tobacco stands, in
December 25 was sowed, and the seed is made to immersion in the strengthening agent aqueous solution in embodiment 3 and handled within 30 minutes, is taken out simultaneously
After drying, sowed according to floating seedlings technical standard and nursery, environment temperature exists in 6 DEG C -18 DEG C of number of days during nursery
80% or so.
It is control that the present embodiment sets following 5 parallel processing solution simultaneously, and seed soak time is 30min, respectively
For：
Control 1, Seed soaking；
Control 2, with 1% lime water solution seed soaking；
Control 3, is soaked seed with the 1% plant ash aqueous solution；
Control 4, is soaked seed with the 1% bacillus subtilis aqueous solution；
Control 5,100% ginkgo biloba extract is directly soaked seed.
In above-mentioned experimental example and control, the preceding 4d after emerging, daily investigation records germination and emergence situation, until having emerged
Entirely, germination percentage and germinating energy are calculated；7d takes 10 plants of tobacco seedlings at random before transplanting, and claims fresh weight, and is finished 30 minutes at 105 DEG C, then
Dried at 80 DEG C, be cooled to room temperature and claim dry weight, calculate Ratio of dry to fresh matter of leaves, press《According to tobacco cultivation》(Liu Guoshun, Beijing：Chinese agriculture
Publishing house, 2012 the 7th time printing) flue-cured tobacco strong sprout standard progress investigation seedling quality, calculate strong sprout rate；Meanwhile, each processing
Investigate 5m2Tobacco seedlings morbidity strain, calculates diseased plant rate.It is recorded in table 1 below.
The germination situation of 1 different disposal cloud and mist of table 87 and seedling quality index
Knowable to upper table 1, the tobacco seed after strengthening agent of the present invention processing, its germinating energy, germination percentage, germination
Index, planting percent, strong sprout rate are respectively higher than remaining control treatment 3.1%-5.8%, 3.6%-6.5%, 6.1-14.6,4.6%-
6.0%th, 2.9%-4.8%, the tobacco seedlings Ratio of dry to fresh matter of leaves into seedling stage is less than control 0.30-0.75, and diseased plant rate is less than control 2.16%-
4.26%, shorten into seedling stage 5-6d.
Embodiment 2
It is K326, floating seedlings greenhouse of the sowing place in Agricultural University Of Hunan's tobacco teaching test base for planting experimentally son
It is interior, sowed within 25th December, the seed is made in embodiment 3 after being soaked 25 minutes in the strengthening agent aqueous solution, taken
After going out and drying, sowed according to floating seedlings technical standard and nursery, day of the environment temperature at 6 DEG C -16 DEG C during nursery
Number is more than 75%.
It is control that the present embodiment sets following 5 parallel processing solution simultaneously, and seed soak time is 25min, respectively
For：
Control 1, Seed soaking；
Control 2, with 1% lime water solution seed soaking；
Control 3, is soaked seed with the 1% plant ash aqueous solution；
Control 4, is soaked seed with the 1% bacillus subtilis aqueous solution；
Control 5,100% ginkgo biloba extract is directly soaked seed.
In above-mentioned experimental example and control, the preceding 4d after emerging, daily investigation records germination and emergence situation, until having emerged
Entirely, germination percentage and germinating energy are calculated；7d takes 10 plants of tobacco seedlings at random before transplanting, and claims fresh weight, and is finished 30 minutes at 102 DEG C, then
Dried at 70 DEG C, be cooled to room temperature and claim dry weight, calculate Ratio of dry to fresh matter of leaves, press《According to tobacco cultivation》(Liu Guoshun, Beijing：Chinese agriculture
Publishing house, 2012 the 7th time printing) flue-cured tobacco strong sprout standard progress investigation seedling quality, calculate strong sprout rate；Meanwhile, each processing
Investigate 5m2Tobacco seedlings morbidity strain, calculates diseased plant rate.It is recorded in table 2 below.
The different disposal K326 germinations situation of table 2 and seedling quality index
Knowable to upper table 2, the tobacco seed after strengthening agent technical finesse of the present invention, its germinating energy, germination percentage,
Germination index, planting percent, strong sprout rate be respectively higher than remaining control 3.8%-5.8%, 4.1%-6.6%, 10.0-14.8,
4.7%-7.3%, 5.1%-7.0%, the tobacco seedlings Ratio of dry to fresh matter of leaves into seedling stage is less than control 0.37-0.83, and diseased plant rate is less than control
2.1%-4.3%, shortens into seedling stage 6-7d.
Embodiment 3
It is cloud and mist 99, floating seedlings greenhouse of the sowing place in Agricultural University Of Hunan's tobacco teaching test base for planting experimentally son
It is interior, sowed within 25th December, the seed is made to immersion in the strengthening agent aqueous solution in embodiment 3 and handled within 30 minutes,
After taking out and drying, sowed according to floating seedlings technical standard and nursery, environment temperature is at 6 DEG C -16 DEG C during nursery
Number of days is more than 75%.
It is control that the present embodiment sets following 5 parallel processing solution simultaneously, and seed soak time is 30min, respectively
For：
Control 1, Seed soaking；
Control 2, with 1% lime water solution seed soaking；
Control 3, is soaked seed with the 1% plant ash aqueous solution；
Control 4, is soaked seed with the 1% bacillus subtilis aqueous solution；
Control 5,100% ginkgo biloba extract is directly soaked seed.
In above-mentioned experimental example and control, the preceding 4d after emerging, daily investigation records germination and emergence situation, until having emerged
Entirely, germination percentage and germinating energy are calculated；7d takes 10 plants of tobacco seedlings at random before transplanting, and claims fresh weight, and is finished 30 minutes at 102 DEG C, then
Dried at 70 DEG C, be cooled to room temperature and claim dry weight, calculate Ratio of dry to fresh matter of leaves, press《According to tobacco cultivation》(Liu Guoshun, Beijing：Chinese agriculture
Publishing house, 2012 the 7th time printing) flue-cured tobacco strong sprout standard progress investigation seedling quality, calculate strong sprout rate；Meanwhile, each processing
Investigate 5m2Tobacco seedlings morbidity strain, calculates diseased plant rate.It is recorded in table 3 below.
The germination situation of 3 different disposal cloud and mist of table 99 and seedling quality index
As known from Table 3, the tobacco seed after strengthening agent of the present invention processing, its germinating energy, germination percentage, germination refer to
Number, planting percent, strong sprout rate be respectively higher than each control 4.8%-7.2%, 5.9%-7.2%, 12.1-17.9,4.2%-8.7%,
3.6%-9.5%, the tobacco seedlings Ratio of dry to fresh matter of leaves into seedling stage is less than control 0.60-1.25, and diseased plant rate is less than control 2.06%-
5.86%, shorten into seedling stage 6-8d.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of tobacco seed sprouts strong sprout composition, it is characterised in that include the component of following parts by weight：Quick lime 80-150
Parts by weight, bacillus subtilis microbial inoculum 0.5-1.5 parts by weight, plant ash 80-200 parts by weight, ginkgo biloba extract 5-15 parts by weight.
2. tobacco seed according to claim 1 sprouts strong sprout composition, it is characterised in that include the group of following parts by weight
Point：The parts by weight of quick lime 100, the parts by weight of bacillus subtilis microbial inoculum 1, the parts by weight of plant ash 150, the parts by weight of ginkgo biloba extract 10.
3. tobacco seed according to claim 1 or 2 sprouts strong sprout composition, it is characterised in that the bacillus subtilis
Microbial inoculum is the bacillus subtilis microbial inoculum WP that bacteria containing amount is higher than 10,000,000,000 living spores/g.
4. the tobacco seed according to claim any one of 1-3 sprouts strong sprout composition, it is characterised in that the ginkgo carries
It is ginkgo leaf and/or ginkgo nut pericarp extract to take thing.
5. the tobacco seed according to claim any one of 1-4 sprouts strong sprout composition, it is characterised in that the ginkgo carries
Thing is taken to extract as follows：Dry ginkgo leaf and/or ginkgo nut pericarp is taken to crush, according to 1：3.5-5.5 mass ratio
Mixed with 70-80wt% ethanol solution, 4-5h is extracted at a temperature of 40-45 DEG C, and extract solution is concentrated into moisture content be less than
10% paste, is produced.
6. the tobacco seed described in claim any one of 1-5, which sprouts strong sprout composition, to be used to prepare tobacco seed sprouting strengthening agent
Purposes.
7. a kind of tobacco seed sprouts strengthening agent, it is characterised in that sprouted as the tobacco seed described in claim any one of 1-5
Hair strong sprout composition adds water the aqueous solution being made.
8. tobacco seed according to claim 7 sprouts strengthening agent, it is characterised in that the tobacco seed sprouts strong sprout group
The mass concentration that compound accounts for the aqueous solution is 0.5%-2.5wt%.
9. a kind of method that tobacco seed for preparing described in claim 7 or 8 sprouts strengthening agent, it is characterised in that including according to
It is selected to measure the step of quick lime, bacillus subtilis microbial inoculum, plant ash, ginkgo biloba extract are mixed, and it is obtained to be dissolved in water
The aqueous solution of selected mass concentration, is produced.
10. a kind of tobacco seed described in claim 7 or 8 sprouts the application method of strengthening agent, it is characterised in that including by cigarette
Grass seed is placed in the tobacco seed and sprouts the step of being soaked 15-45 minutes in strengthening agent, dries water.
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| CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
| CN113519563A (en) * | 2021-05-12 | 2021-10-22 | 湖南省烟草公司邵阳市公司 | Tobacco seedling-reviving and growth-promoting composition, preparation and using method thereof |
| CN115141779A (en) * | 2022-07-26 | 2022-10-04 | 贵州大学 | Growth-promoting bacterium for promoting tobacco seedling growth and application thereof |
| CN116814479A (en) * | 2023-06-08 | 2023-09-29 | 贵州大学 | Bacillus GM-12-PT and method for promoting germination of Pterocarpus marsupium seeds by using same |
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