CN115141779B

CN115141779B - Growth-promoting bacterium for promoting growth of tobacco seedlings and application thereof

Info

Publication number: CN115141779B
Application number: CN202210888712.6A
Authority: CN
Inventors: 黄莺; 王小花; 丁梦娇; 李英民; 代文典; 宋绍光; 刘仁祥
Original assignee: Guizhou University
Current assignee: Guizhou University
Priority date: 2022-07-26
Filing date: 2022-07-26
Publication date: 2023-04-07
Anticipated expiration: 2042-07-26
Also published as: CN115141779A

Abstract

The invention discloses a growth-promoting bacterium for promoting the growth of tobacco seedlings, which is preserved in China center for type culture Collection (CCTCC NO) at 7/19/2021 with the preservation number of CCTCC NO: m2021895, classification name: brevibacterium antarcticaccumwn 5; the invention is screened from soil for tobacco planting in Weining of Guizhou, is applied in the floating seedling process of flue-cured tobacco, has better growth promoting function, particularly obviously promotes the growth of overground parts, and enhances the resistance of tobacco seedlings.

Description

Growth-promoting bacterium for promoting growth of tobacco seedlings and application thereof

Technical Field

The invention relates to the technical field of tobacco seedling growth, in particular to growth-promoting bacteria for promoting the growth of tobacco seedlings and application thereof.

Background

The flue-cured tobacco seeds are extremely small, the difference between seedlings and adult plants is large, and the robust seedlings are the basic guarantee for realizing high quality and good yield of the flue-cured tobacco. Therefore, strong seedlings are usually cultivated by adopting a seedling transplanting mode. At present, the internationally common flue-cured tobacco seedling raising modes mainly comprise nutrition tray seedling raising and floating seedling raising, the two methods are carried out indoors, and the seedling rate is high due to controllable temperature, humidity and nutrient components. However, these seedling raising methods also have problems, such as damping-off, etc. easily occurring in the bed stage; during transplanting, seedlings are easily affected by adverse climate and the viability is affected. At present, the above problems are solved by adopting methods such as seedbed disinfection, well cellar type transplanting and the like, and the situation is relieved. But improving the quality of flue-cured tobacco seedlings and enhancing the resistance of the flue-cured tobacco seedlings is the method for controlling the root cause.

The soil probiotics have the main functions of promoting the release of elements such as nitrogen, phosphorus and potassium, stimulating the growth of crops, killing insects and sterilizing, increasing the resistance of crops and the like, and the types which are common at present comprise bacillus subtilis, bacillus megaterium, bacillus thuringiensis, bacillus licheniformis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, azotobacter vinelandii, mycorrhizal fungi, lactobacillus and the like. The research on the screening, function definition and applicable environment of soil beneficial bacteria is always a research hotspot of agricultural production since the last 80 centuries.

Microorganisms have certain selectivity on ecological environment, and although a large amount of soil-beneficial microbial agents or fertilizers appear in the market, the effect can be good only in a limited area, so that the wide popularization of beneficial microorganisms is difficult.

Disclosure of Invention

Aiming at the problem that the application range of the existing microbial preparation or biological fertilizer is not wide, the invention screens soil beneficial microorganisms suitable for the flue-cured tobacco floating seedling environment, promotes the nutrient absorption of floating seedling, improves the overground part growth, improves the tobacco seedling resistance and overcomes the defects of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: the growth-promoting bacterium for promoting the growth of tobacco seedlings is preserved in China Center for Type Culture Collection (CCTCCNO) at 7-19.2021, with the preservation number being CCTCCNO: m2021895, classification name: brevibacterium antarcticumWN5; after the detection of 8 months 3 in 2021, the result is survival after the detection by the China center for type culture Collection.

Application of growth-promoting bacteria for promoting tobacco seedling growth in tobacco seedling bed stage is provided.

The invention has the beneficial effects that the tobacco is screened from soil for tobacco planting in Weining of Guizhou, is applied in the floating seedling process of flue-cured tobacco, has better growth promoting function, particularly obviously promotes the growth of overground parts, and enhances the resistance of tobacco seedlings.

Drawings

FIG. 1 shows catalase activity of tobacco seedlings after microbial inoculum application;

FIG. 2 shows the soluble protein content of tobacco seedlings after the microbial inoculum is applied;

FIG. 3 shows the nitrate reductase activity of tobacco seedlings with microbial inoculum applied.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the present embodiment provides a growth-promoting bacterium for promoting the growth of tobacco seedlings so as to facilitate a more intuitive understanding of the present technical solution;

1. design of experiments

Adopting a single-factor test, setting WN5 microbial inoculum application treatment in the flue-cured tobacco seedling bed period, taking no microbial inoculum as a Control (CK), and repeating for 3 times. Wherein the viable count of WN5 microbial inoculum is more than or equal to 100 hundred million g ^-1 。

The seedling raising trays in the floating seedling raising pond are separated by white plastic cloth and divided into 6 cells, and 3 160-hole expanded polystyrene floating trays with the specification of 57cm multiplied by 36cm multiplied by 6cm are placed in each cell. The tobacco seedling seeds (coating seeds) are sown in 2022 years, 2 months and 25 days, emerge in 3 months and 10 days, and reach the big cross stage in 3 months and 18 days. The microbial agent is administered during the grand cross phase (i.e., 3 months and 19 days).

WN5 microbial inoculum is diluted to 10 of the original bacterial liquid concentration ^-1 Adding the bacterial agent of each tobacco seedling with the application amount of about 5ml into the seedling substrate. And observing the growth and development conditions of the tobacco seedlings every day after the microbial inoculum is applied, and recording the growth potential of the tobacco seedlings. Selecting 15 representative tobacco seedlings in each cell, recording the fresh weight, dry weight, root crown ratio, plant height, stem circumference, maximum leaf length, maximum leaf width, maximum leaf area, leaf number, chlorophyll content of the first leaf/the second leaf, the maximum root length, root stem/fresh weight, root surface area, root volume and root activity of main root indexes of the tobacco seedlings treated differently, and measuring the physiological indexes of the root system of the tobacco seedlings mainly comprises superoxide dismutase (SOD), catalase (CAT) activity, soluble protein and Nitrate Reductase (NR) activity.

2 assay method

2.1 determination of agronomic traits and root system indexes of tobacco seedlings

Photographing, recording, sampling and measuring are carried out on 26 days of 3 months in 2022. Wherein, the agronomic characters of the tobacco seedlings are determined according to Chinese tobacco industry standard YC/T142-2010; the root surface area and root volume were determined using a root system scanner (model: epson expression 1000 xl); measuring chlorophyll by using a chlorophyll meter; root system activity was measured by triphenyltetrazolium chloride reduction method (TTC method).

2.2 determination of indicators associated with resistance

Representative tobacco seedlings of 15 strains were selected for each cell, and the tobacco seedlings were measured for Catalase (CAT) activity, soluble protein content, and Nitrate Reductase (NR) activity on the day of sampling.

The strain provided by the embodiment is bacterial Bacillus pumilus WN5 (Brevibacterium antarcticam), and is screened from tobacco-planting soil of Weining, guizhou; after the bacteria are applied to the flue-cured tobacco in the big cross stage, the influence on tobacco seedlings is mainly as follows:

promoting the growth of tobacco in seedling stage (Table 1), wherein the maximum leaf area and plant height are obviously improved; the dry and fresh weight of the leaves is increased, and the substance accumulation is improved; the tobacco seedling resistance is enhanced, which is mainly characterized in that the catalase activity (figure 1) and the soluble protein content (figure 2) in the tobacco leaves are obviously increased; nitrate reductase activity was increased (fig. 3), and the ability of tobacco seedlings to absorb nutrients was increased.

TABLE 1 agronomic characters and root system indexes of tobacco seedlings after 7d treatment by beneficial bacteria

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Claims

1. A growth-promoting bacterium for promoting tobacco seedling growth is characterized in that: the growth-promoting bacterium is preserved in China Center for Type Culture Collection (CCTCC) at 7/19/2021, with the preservation number of CCTCC NO: m2021895, classification name: brevibacterium antarcticumWN5.

2. Use of the growth-promoting bacterium according to claim 1 for promoting the growth of tobacco seedlings in the bed stage of tobacco seedlings.