CN109896900A - A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer - Google Patents
A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer Download PDFInfo
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Abstract
The present invention relates to the preparations and application of a kind of complex micro organism fungicide and biological organic complex fertilizer.The present invention is formed in conjunction with gas chromatography and inorganic matter by scientific formula using bacillus, saccharomycete, photosynthetic bacteria etc..It is the ecosystem and nutrient environment for improving crop rhizosphere soils by the interaction between microorganism that it, which is acted on, improves crop absorbability to promote the growth of crop and achievees the effect that increasing both production and income.The present invention has effects that improve soil, prevents and treats soil-borne disease insect pest, the degradation hazard residues such as heavy metal and pesticide, promotes plant growth, improves crop anti-adversity and increase crop quality, has the characteristics that efficient, fast effect, holding effect.
Description
Technical field:
The invention belongs to soil microbiology fields, and in particular to a kind of complex micro organism fungicide and biological organic complex fertilizer
Preparation and application.
Background technique:
China is large agricultural country, and agricultural planting area is huge, in agricultural production in order to which increasing both production and income, chemical fertilizer and growth swash
Element etc. promotes the product of plant growth largely to be used extensively.Pesticide disease and pest control is largely used simultaneously.If things go on like this, seriously
Soil texture is destroyed, keeps organic matter in soil and beneficial microbe poor, pest and disease damage source cannot be effectively suppressed, in turn
Lead to the deterioration of the ecological environment, the serious sustainable development for restricting agricultural promotes the hazard residue substance of crop to increase, product quality
Constantly decline seriously endangers human health.
The appearance of microbial-bacterial fertilizer makes agricultural planting not be completely dependent on chemical fertilizer and pesticide, by the effect of microorganism, no
Soil, disease and insect resistance can be only improved, degrading pesticide residues eliminate poisoning of drug, phytotoxicity, and more and more peasants understand, recognize
Use the necessity of microbial-bacterial fertilizer.Bacterial manure is pure in some drawbacks in the market at home: 1, bacterium number is single, and some bacterial manure only has one
Kind or two kinds of bacterium；2, bacterium vigor is weak, and life time is short, and quantity is also seldom；3, effect is small, no persistence, First Year using effect
It can be with second year, which continues to use, does not have effect；4, individual bacterial manure contain hormone, and effect was obvious at that time, and publicity is not achieved later
Effect.
Agrochemical, pesticide and microbial-bacterial fertilizer there are aiming at the problem that provide and a kind of be suitable for vegetable and fruit and farming
The safe and efficient fertilizer of object.
Summary of the invention:
It is an object of the invention to combine the ecological balance of plant rhizosphere theoretical, to solve existing agricultural problem as target,
The preparation and application of complex micro organism fungicide and biological organic complex fertilizer on the basis of waste resource recovery recycles.
To achieve the above object, the invention adopts a technical scheme as:
A kind of composite microbial bacteria, composite microbial bacteria are bacillus, saccharomycete and photosynthetic bacteria；Wherein, gemma bar
Bacterium, saccharomycete and photosynthetic bacteria, which are pressed, waits mass ratioes mixing；The bacillus be bacillus subtilis, bacillus megaterium,
Clothing bacillus, bacillus laterosporus, Paenibacillus polymyxa, bacillus amyloliquefaciens, soaks numb bud at bacillusmusilaginosiengineering
One or more of spore bacillus, bacillus thuringiensis, Bacillus cercus, series bacillus；The saccharomycete is to produce protein
One or more of Candida, saccharomyces cerevisiae, C. pelliculosa, Candida lipolytica, Saccharomyces paradoxus；It is described photosynthetic thin
Bacterium is one or more of Rhodopseudomonas palustris, colloid Rhodopseudomonas, hydrogenlike silicon ion.
Above-mentioned each bacterial strain is provided by Beijing Culture Collection Center, and bacterium numbering is bacillus subtilis:
ACCC03120；Bacillus megaterium: ACCC02979；Bacillus licheniformis: ACCC19747；Gel-shaped gemma bar
Bacterium: CICC21700；Bacillus laterosporus: ACCC05440；Paenibacillus polymyxa: CICC24260；Liquefying starch gemma bar
Bacterium: ACCC60428；Bacillus thuringiensis:
ACCC19967；Bacillus macerans: ACCC 01910；Bacillus cercus: ACCC 01513；Series bacillus:
ACCC 01973；Candida utili: ACCC 20059；Candida lipolytica:
CICC31223；Saccharomyces cerevisiae: CGMCC2.3854；C. pelliculosa: CGMCC2.1501；Saccharomyces paradoxus:
CGMCC2.4295；Rhodopseudomonas palustris: CGMCC1.2180；Colloid red pseudomonas: ACCC 11673；Hydrogenlike silicon ion:
CGMCC1.16068。
The bacillus be bacillus subtilis, bacillus megaterium, bacillus licheniformis, bacillusmusilaginosiengineering,
Bacillus laterosporus, Paenibacillus polymyxa, bacillus amyloliquefaciens, bacillus thuringiensis；The saccharomycete is to produce protein
Candida, saccharomyces cerevisiae, C. pelliculosa, Candida lipolytica；The photosynthetic bacteria is Rhodopseudomonas palustris, colloid
Rhodopseudomonas, hydrogenlike silicon ion.
Fermented and cultured to bacillus and saccharomycete spore count reaches 80% to the bacterial strain respectively, and light and bacterium bacteria concentration reach
To 1010A/ml or more is then mixed according to isometric ratio.
A kind of complex micro organism fungicide, complex micro organism fungicide according to parts by weight, bacterium solution 4-10 described in claim 1
Part；80-95 parts of adsorbent；5-15 parts of protective agent.
The complex micro organism fungicide according to parts by weight, described bacterium solution 6-10 parts；85-95 parts of adsorbent；Protective agent 8-
13 parts.
The adsorbent be wheat cake powder, wheat bran, bacteria residue, bentonite, rotten mosses ash, flyash, one or more of；It is preferred that
It is wheat cake powder, bacteria residue, wheat bran, bentonite, rotten mosses ash.The protective agent is starch, vitamin C, lactose, molasses, soybean protein
One or more of；Preferably starch, vitamin C, soybean protein.
Remaining culture medium waste crushed material after wheat cake powder system locality Cordyceps militaris (northern Chinese caterpillar Fungus) production harvest, wheat
In cake powder containing largely the wheat powder that are not fully used and Chinese caterpillar fungus mycelia and its metabolite (cordycepic acid, cordycepin,
Cordyceps sinensis polysaccharide etc.).
Bacterium solution, adsorbent and protective agent are mixed according to aforementioned proportion, are stirred by a kind of preparation method of complex micro organism fungicide
It mixes uniformly；Powder processed is dried, viable count is effective and reaches 2-9 × 109A/gram to get microbial inoculum.
A kind of biological organic complex fertilizer is made of following raw material (weight): described bacterium solution 4-10 parts；Organic matter
60-85 parts；5-15 parts of inorganic matter.
It is preferred that being made of following raw material (weight): described bacterium solution 6-10 parts；70-80 parts of organic matter；Inorganic matter 7-
10 parts.
The organic matter is one or more of feces of livestock and poultry, stalk, wheat cake, bacteria residue, vinasse.The inorganic matter is sulphur
Sour potassium, phosphamide zinc, calcium chloride, boron mud, calcium molybdate, one or more of containing manganese superphosphate.
Wheat cake system locality Cordyceps militaris (northern Chinese caterpillar Fungus) produces remaining culture medium waste after harvest, contains in wheat cake
The wheat and Chinese caterpillar fungus mycelia and its metabolite (cordycepic acid, cordycepin, Cordyceps sinensis polysaccharide etc.) not being fully used largely.
The fermentation waste of certain pharmaceutical factory of locality, bacteria residue system, battalion needed for a large amount of beneficial microbes, metabolite and its fermentation is contained in bacteria residue
Support object.Remaining grain residue after the wine brewing of vinasse system locality winery.It is saccharomycete rich in vinasse, crude protein, a variety of
Microelement, vitamin and a variety of amino acid etc..
A kind of preparation of biological organic complex fertilizer,
1) organic matter is mixed through sterilizing after compost maturity, it then will decomposed organic matter afterwards and inorganic according to aforementioned proportion
Object mixing, is dried after mixing well, and is crushed, it is spare that fineness reaches 60-80 mesh；
2) bacterium solution is added on step 1) preliminary mixture and is mixed well, be sent into blender stirring；It is to be mixed equal
After even, it is re-fed at 30-60 DEG C of roller pelletizer and is granulated to get compound fertilizer.
For the organic compound fertilizer during compost, microorganism and its metabolite in wheat cake, bacteria residue and vinasse can
To accelerate digest process, and a variety of nutrients contained therein can either promote the breeding of microorganism that plant can be promoted raw
It is long.
Advantage for present invention:
Microbial inoculum of the invention is combined using bacillus, saccharomycete, photosynthetic bacteria etc., and active height, effect are strong, act on
Persistently, the features such as life cycle is long；Microbial inoculum combination gas chromatography and inorganic matter are then formed into life by scientific formula again
Object organic compound fertilizer；Compound fertilizer's effect is to improve the ecosystem of crop rhizosphere soils by the interaction between microorganism
System and nutrient environment improve crop absorbability to promote the growth of crop and achieve the effect that increasing both production and income.
Compound fertilizer of the present invention has improvement soil, prevention and treatment soil-borne disease insect pest, the degradation hazard residues such as heavy metal and pesticide, promotees
The effect of into plant growth, raising crop anti-adversity and increasing crop quality has the characteristics that efficient, fast effect, holding effect.It solves
Resource utilization, the microbial-bacterial fertilizer effect of local existing industrial and agricultural waste be not lasting and the use misgivings of peasant's microbial manure
And the problems such as consumption pressure, and a kind of efficient, effect, holding effect fastly Safe fertilizer is provided for the sustainable development of agricultural, simultaneously
Save the cost reduces the pressure of peasant.
Detailed description of the invention:
Fig. 1 is the flow sheet of complex micro organism fungicide and biological organic complex fertilizer that present example provides.
Specific example
Below with reference to specific example, the present invention is further illustrated, but scope of the invention is not limited in
These examples.
Embodiment 1
This example bacterium solution are as follows: bacillus subtilis, bacillus megaterium, bacillus laterosporus, Paenibacillus polymyxa, Soviet Union
Cloud gold bacillus, candida utili, Rhodopseudomonas palustris composition, each group are 1:1:1:1:1:1:1 at volume ratio,
The spore count of middle bacillus and saccharomycete reaches 80%, and photosynthetic bacteria viable count reaches 1010Cfu or more.
This example microbial inoculum are as follows: by weight, 6 parts of above-mentioned bacterium solution, adsorbent: 90 parts；Protective agent: 8 parts.
Adsorbent is 20 parts of wheat cake powder, 20 parts of wheat bran, 30 parts of bentonite, 20 parts of rotten mosses ash according to parts by weight；
Protective agent: 3 parts of starch, 2 parts of vitamin C, 3 parts of soybean protein.
This example compound fertilizer are as follows: by weight, 6 parts of bacterium solution；68 parts of organic matter；8 parts of inorganic matter.Organic matter is by weight
Number meter, 41 parts of feces of livestock and poultry, 13 parts of stalk, 4 parts of wheat cake, 5 parts of vinasse, 5 parts of bacteria residue；The stalk should be crushed to 5-10cm after
Stalk.
Inorganic matter according to parts by weight, 2 parts of potassium sulfate, 2 parts of phosphamide zinc, 1 part of calcium chloride, 1 part of boron mud, 1 part of calcium molybdate,
Containing 1 part of manganese superphosphate.
The preparation of organic fertilizer:
1) by the prior art by bacillus subtilis, bacillus megaterium, bacillus laterosporus, Paenibacillus polymyxa,
Bacillus thuringiensis, candida utili, Rhodopseudomonas palustris activate respectively, each after then activating according to the prior art
The carry out fermented and cultured of bacterial strain, until the spore count of bacillus and saccharomycete reaches 80%, photosynthetic bacteria viable count reaches for culture
To 1010Cfu or more；For use.
2) bacterial strain after above-mentioned each fermented and cultured is mixed evenly according to isometric ratio, i.e. acquisition bacterium solution,
It is spare.
3) bacterium solution, adsorbent, protective agent are mixed in above-mentioned microbial inoculum ratio according to the ratio, are stirred evenly, then dry powder processed,
Resampling detection is effective viable count and reaches 4X109It is a/gram both qualified products, dispense finished product to get microbial inoculum.
4) in above-mentioned compound fertilizer's ratio by feces of livestock and poultry, stalk, wheat cake is fast using the prior art after vinasse and bacteria residue mixing
Sterilize after fast compost maturity, examined no pathogenic bacteria and miscellaneous bacteria rate less than 1% after, then mixed according to the above ratio with inorganic matter,
It dries, crushes, fineness reaches 60 mesh or so, spare after mixing well；
5) bacterium solution and step 4) mixture is obtained according to the above ratio to mix well into blender stirring；
6) uniformly rear crushing to be mixed, is re-fed into roller pelletizer, temperature is controlled at 40 DEG C；It is granulated the fertilizer come out, point
Finished product is dressed up to get organic fertilizer.
Application examples 1
The complex micro organism fungicide 5g for taking example to obtain is added in the conical flask equipped with 100ml sterile water and mixes
It is even that bacteria suspension is made, bacterial suspension inoculation is indicated to cultivate on bacterium culture medium in the Different Kinds of Pathogens for being coated with moderate concentration, is measured to each
The bacteriostatic activity of crop disease.It the results are shown in Table 1.It is hanged the above-mentioned bacterium prepared is placed in bradysia odoriphaga from the collected fresh and alive nematode in field
3s is dipped in liquid, is then raised in 30 DEG C of room temperatures again, and the insecticidal effect to nematode and bradysia odoriphaga is measured.It is shown in Table 2.
Bacteriostatic test of 1. complex micro organism fungicide of table to a variety of cause of disease indicator bacterias
Note :+indicate that antibacterial band is less than 1cm, ++ indicate that antibacterial band is greater than 1cm
2 complex micro organism fungicide of table tests the inhibition of a variety of insect pests
| Soybean cyst nematode Heterodera glycines | Cucumber root-knot nematode | Wheat anguina | Bradysia odoriphaga |
| Heterodera glycines | Meloidogyne Goeld | Anguina tritici | Bradsia odoriphaga |
| 98% | 96% | 96% | 97% |
Had the function of inhibiting various plants pathogen by the visible present example complex micro organism fungicide of above-mentioned table 1 and 2,
And function and effect are obvious；There is killing effect to a variety of nematodes and bradysia odoriphaga insect pest of the plant, function and effect are 96% or more.
Biological organic complex fertilizer obtained in embodiment is administered in the plantation of cucumber, is embodied as follows: by cucumber
Model field is divided into two groups, control group and experimental group, and in cucumber field planting, control group conventional fertilizer application, the experimental group application present invention is real
The biological organic complex fertilizer of example preparation.The indices that cucumber is measured after field planting 45 days, the results are shown in Table 4., wherein opposite chlorophyll
Content is measured using chlorophyll measuring apparatus.
The indices of 3. cucumber of table
| Plant height cm | Base portion stem thickness cm | Relative chlorophyll content | Root/shoot ratio | |
| Experimental group | 43 | 11 | 406.66 | 8.6 |
| Control group | 32 | 8.7 | 348.98 | 6.3 |
This test display shows that present example can obviously accelerate cucumber crop growth, increases crop picking time, improves
Crop quality decomposes poisonous and harmful substance.Use the cucumber of present example biological organic complex fertilizer in bright-colored degree
On it is more light green, there is preferable faint scent and mouthfeel more preferably；The cucumber that two groups of tests are grown is placed in together respectively
Under equal environment, the resting period using the cucumber of present example biological organic complex fertilizer is 2-5 days longer, cooperates composite microbial
Object microbial inoculum application effect is more preferably.
Embodiment 2
This example bacterium solution are as follows: bacillus subtilis, bacillus licheniformis, gel-shaped gemma bar, Paenibacillus polymyxa, wine
Brewer yeast, hydrogenlike silicon ion composition, each group are 1:1:1:1:1:1 at volume ratio, wherein the spore of bacillus and saccharomycete
Number reaches 80% or more, and photosynthetic bacteria viable count reaches 1010Cfu or more.
This example microbial inoculum are as follows: by weight, 8 parts of bacterium solution, adsorbent: 87 parts；Protective agent: 12 parts.
Adsorbent is 21 parts of wheat cake powder, 24 parts of wheat bran, 17 parts of bacteria residue, 25 parts of rotten mosses ash according to parts by weight；
Protective agent: 3 parts of molasses, 2 parts of vitamin C, 4 parts of soybean protein, 3 parts of lactose.
This example compound fertilizer are as follows: by weight, 8 parts of bacterium solution；80 parts of organic matter；6 parts of inorganic matter.Organic matter is by weight
Number meter, 45 parts of feces of livestock and poultry, 15 parts of stalk, 7 parts of wheat cake, 5 parts of vinasse, 8 parts of bacteria residue；The stalk should be crushed to 5-10cm after
Stalk
Inorganic matter according to parts by weight, 1 part of potassium sulfate, 1.5 parts of phosphamide zinc, 1 part of calcium chloride, 1 part of boron mud, calcium molybdate 1
Part, contain 0.5 part of manganese superphosphate.
Preparation:
1) by the prior art by bacillus subtilis, bacillus licheniformis, gel-shaped gemma bar, Paenibacillus polymyxa,
Saccharomyces cerevisiae, hydrogenlike silicon ion activate respectively, then cultivate the carry out fermented and cultured of bacterial strain each after activation according to the prior art
Until the spore count of bacillus and saccharomycete reaches 80%, photosynthetic bacteria viable count reaches 1010Cfu or more, for use.
2) bacterial strain after above-mentioned each fermented and cultured is mixed evenly according to isometric ratio, it is spare.
3) bacterium solution, adsorbent, protective agent are mixed in above-mentioned microbial inoculum ratio according to the ratio, are stirred evenly, then dry powder processed,
Resampling detection is effective viable count and reaches 5X109It is a/gram qualified products, dispense finished product to get microbial inoculum.
4) in above-mentioned compound fertilizer's ratio by feces of livestock and poultry, stalk, wheat cake is fast using the prior art after vinasse and bacteria residue mixing
Sterilize after fast compost maturity, examined no pathogenic bacteria and miscellaneous bacteria rate less than 1% after, then mixed according to the above ratio with inorganic matter,
It dries, crushes, fineness reaches 60 mesh or so, spare after mixing well；
5) bacterium solution and step 4) mixture is obtained according to the above ratio to mix well into blender stirring；
6) uniformly rear crushing to be mixed, is re-fed into roller pelletizer, temperature is controlled at 40 DEG C；It is granulated the fertilizer come out, point
Finished product is dressed up to get biological organic fertilizer compound fertilizer.
Application examples 2
The complex micro organism fungicide and biological organic complex fertilizer that embodiment is obtained are in Mr. Yu's maize field experiment.Test
Concrete condition is as follows: crop field being divided into three regions, does control group, group I and experimental group II, group I application respectively
Biological organic complex fertilizer of the present invention, control group are compound fertilizer.Corn yield data are shown in Table 4.
4. corn yield data of table
| Spike length/cm | Grain number per spike/ | 100-grain weight/g | Illness rate/% | Roll over hectare yield/kg | |
| Control group | 22.7 | 592 | 41.5 | 3.5 | 10190 |
| Group I | 22.9 | 581 | 43.2 | 1.6 | 11056 |
| Control group II | 23.1 | 619 | 42.7 | 1.3 | 11721 |
The complex micro organism fungicide and biological organic complex fertilizer of experimental result present example can promote corn seed
Root system development, makes root fast-growth, will use from corn (seed) germination and emergence and each growth period until autumn harvest
This example fertilizer is pulled out with the corn not used, and observation root is one times nearly all poor.The aerial root of corn is obviously more, corn
There is no an ear barren tip, and can straw staygreen, it is mature after corn relative starch content it is higher.
Embodiment 3
This example bacterium solution are as follows: bacillusmusilaginosiengineering, bacillus laterosporus, bacillus amyloliquefaciens, Su Yunjin gemma
Bacillus, Candida lipolytica, colloid Rhodopseudomonas composition, each group are 1:1:1:1:1:1 at volume ratio, wherein bacillus
Reach 80% with the spore count of saccharomycete, photosynthetic bacteria viable count reaches 1010Cfu or more.
This example microbial inoculum are as follows: by weight, 10 parts of bacterium solution, adsorbent: 92 parts；Protective agent: 10 parts.
Adsorbent is 21 parts of wheat cake powder, 18 parts of bacteria residue, 30 parts of bentonite, 23 parts of rotten mosses ash according to parts by weight；
Protective agent: 3.5 parts of starch, 2.5 parts of vitamin C, 3 parts of soybean protein, 1 part of molasses.
This example compound fertilizer are as follows: by weight, 10 parts of bacterium solution；72 parts of organic matter；7 parts of inorganic matter.Organic matter is by weight
Number meter, 40 parts of feces of livestock and poultry, 11 parts of stalk, 3 parts of wheat cake, 13 parts of bacteria residue, 5 parts of vinasse；The stalk, which should be, is crushed to 5-
Stalk after 10cm.
Inorganic matter according to parts by weight, 2 parts of potassium sulfate, 1 part of phosphamide zinc, 2 parts of calcium chloride, 1 part of boron mud, 1 part of calcium molybdate.
Preparation:
1) prior art is pressed by bacillusmusilaginosiengineering, bacillus laterosporus, bacillus amyloliquefaciens, Su Yunjin gemma
Bacillus, Candida lipolytica, colloid Rhodopseudomonas activate respectively, then according to the prior art by the progress of bacterial strain each after activation
Fermented and cultured, until the spore count of bacillus and saccharomycete reaches 80%, photosynthetic bacteria viable count reaches 10 for culture10Cfu with
On, for use.
2) bacterial strain after above-mentioned each fermented and cultured is mixed evenly according to isometric ratio, it is spare.
3) bacterium solution, adsorbent, protective agent are mixed in above-mentioned microbial inoculum ratio according to the ratio, are stirred evenly, then dry powder processed,
Resampling detection is effective viable count and reaches 4X109It is a/gram both qualified products, dispense finished product.
4) in above-mentioned compound fertilizer's ratio by feces of livestock and poultry, stalk, wheat cake is fast using the prior art after vinasse and bacteria residue mixing
Sterilize after fast compost maturity, examined no pathogenic bacteria and miscellaneous bacteria rate less than 1% after, then mixed according to the above ratio with inorganic matter,
It dries, crushes, fineness reaches 60 mesh or so, spare after mixing well；
5) bacterium solution and step 4) mixture is obtained according to the above ratio to mix well into blender stirring；
6) uniformly rear crushing to be mixed, is re-fed into roller pelletizer, temperature is controlled at 45 DEG C；It is granulated the fertilizer come out, point
Dress up finished product.
Application examples 3
Complex micro organism fungicide and biological organic complex fertilizer that embodiment obtains are used in field trial.Test specific feelings
Condition is as follows: experimental planting kind is strawberry, and testing site plot area 30m is arranged2, 3 disposition, respectively control group are set altogether
(CK), experimental group (I) and experimental group (II), the wherein conventional nitrogen-phosphorus-potassium compound fertilizer of control group (CK), experimental group (I) present example
Complex micro organism fungicide, experimental group (II) present example biological organic complex fertilizer.Each disposition is repeated 3 times.Each processing is equal
It is carried out in same time, handling facility etc. is also identical.Test data sheet is shown in Table 5.
The influence that table 5. grows greenhouse strawberry
Face is relatively compareed by the Strawberry Leaves feature that fixed point investigates application complex micro organism fungicide and biological organic complex fertilizer
Color is deepened, and blade is thicker, and the relatively control of florescence and first fruiting period is shifted to an earlier date, and the number of blade, difference in plant height are significant, and the best fruiting period has one
It is fixed to extend, but difference is not significant.In each hillslope processes, experimental group (I), experimental group (II) are compared with control group CK in percentage of fertile fruit, maximum
There is obvious protrusion on fruit mass, single plant fruit number and single plant yield, experimental group (I) is relatively better than experimental group (II)；In vitamin
Content, soluble solid, total reducing sugar and titratable acid etc. also have some superiority.
Embodiment 4
This example bacterium solution are as follows: bacillus subtilis, bacillus laterosporus, Paenibacillus polymyxa, liquefying starch gemma bar
Bacterium, C. pelliculosa, hydrogenlike silicon ion composition, composition ratio are volume ratio 1:1:1:1:1:1, wherein bacillus and yeast
The spore count of bacterium reaches 80%, and photosynthetic bacteria viable count reaches 1010Cfu or more.
Wherein:
This example microbial inoculum are as follows: by weight, 9 parts of bacterium solution, adsorbent: 80 parts；Protective agent: 13 parts.
Adsorbent is 24 parts of wheat bran, 17 parts of bacteria residue, 25 parts of rotten mosses ash, 14 parts of bentonite according to parts by weight；
Protective agent: 3 parts of sucrose, 2.5 parts of vitamin C, 4.5 parts of soybean protein, 3 parts of lactose.
This example compound fertilizer are as follows: by weight, 8 parts of bacterium solution；83 parts of organic matter；9 parts of inorganic matter.Organic matter is by weight
Number meter, 46 parts of feces of livestock and poultry, 14 parts of stalk, 10 parts of wheat cake, 5 parts of vinasse, 8 parts of bacteria residue；The stalk, which should be, is crushed to 5-10cm
Stalk afterwards
Inorganic matter according to parts by weight, 2.5 parts of potassium sulfate, 2 parts of phosphamide zinc, 1 part of calcium chloride, 1.5 parts of boron mud, calcium molybdate
1 part, contain 1 part of manganese superphosphate.
Preparation:
1) prior art is pressed by bacillus subtilis, bacillus laterosporus, Paenibacillus polymyxa, liquefying starch gemma bar
Bacterium, C. pelliculosa, hydrogenlike silicon ion activate respectively, and bacterial strain each after activation is then carried out fermentation training according to the prior art
It supports, until the spore count of bacillus and saccharomycete reaches 80%, photosynthetic bacteria viable count reaches 10 for culture10Cfu or more, to
With.
2) bacterial strain after above-mentioned each fermented and cultured is mixed evenly according to isometric ratio, it is spare.
3) bacterium solution, adsorbent, protective agent are mixed in above-mentioned microbial inoculum ratio according to the ratio, are stirred evenly, then dry powder processed,
Resampling detection is effective viable count and reaches 4X109It is a/gram both qualified products, dispense finished product.
4) according to the above ratio by feces of livestock and poultry, stalk, wheat cake uses prior art high-rate composting after vinasse and bacteria residue mixing
Sterilize after decomposed, examined no pathogenic bacteria and miscellaneous bacteria rate less than 1% after, then mixed according to the above ratio with inorganic matter, through abundant
It dries, crushes, fineness reaches 60 mesh or so, spare after mixing；
5) bacterium solution and step 4) mixture is obtained according to the above ratio to mix well into blender stirring；
6) uniformly rear crushing to be mixed, is re-fed into roller pelletizer, temperature is controlled at 40 DEG C；It is granulated the fertilizer come out, point
Dress up finished product.
Application examples 3
Present example obtains complex micro organism fungicide and biological organic complex fertilizer and tests for soil testing, specific implementation
Are as follows: potting control water law is used, plant factors are eliminated, is the improved effect for preferably embodying soil using flowerpot as ridging container,
Select arid mining soil as experiment soil, using drought-resistant plant (Chinese pine) as experimental plant.Experimental setup 3 processing, point
It Wei not blank control (CK), complex micro organism fungicide (I), biological organic complex fertilizer (II).Wherein, 3 weights are arranged in each disposition
It is multiple, each flowerpot soil 3kg, every basin planting experiment plant one.Plant stability carries out appropriate control water after growing one month, control water
It cuts off the water supply completely after 20 days, normal water supply is measured in experimentation, water is suitably controlled, cuts off the water supply completely and 45 days this 4 differences of cutting off the water supply
The soil moisture content in period, soil texture and the quantity of edaphon, experimental result are shown in Table 5:
Table 5.
Experimental result shows, for moisture content during haveing suffered, the soil moisture content respectively handled is all presented downward trend, but under
The amplitude of drop is different, and certain otherness is presented between each processing；After cutting off the water supply completely, the soil moisture content downward trend of a processing
Obviously slow down.When processing terminate, the soil moisture content respectively handled respectively compared with normal care when have dropped 88.8%, 77.4%,
58.1%.Therefore, the water-retaining property of soil can be improved to a certain extent using complex micro organism fungicide, but the biological organic that compares
The water-retaining property of compound fertilizer is more sufficient.
The soil weight with drought stress degree increase, the soil weight of a processing is all presented fall before after rise become
Gesture, this is because edaphon is relatively abundant under the conditions of normal care and appropriate control water, soil is more under the action of microorganism
Loose, transmissibility and gas permeability are significantly improved, and the soil weight is small；The great decline soil of edaphon becomes after cutting off the water supply completely
Hardened, the soil weight increases.When processing terminate, the soil weight respectively handled respectively compared with normal care when have dropped 1.59%,
9.45%, 11.38%.Therefore, compare blank control, is all had very using complex micro organism fungicide and biological organic complex fertilizer
The effect of good improvement soil texture.
With the increase of drought stress degree, the soil weight respectively handled all presents first to rise to be declined soil porosity afterwards
Trend.When processing terminate, the soil porosity respectively handled respectively compared with normal care when increase 1.26%, 5.65%, 9.48%,
And in the whole process period, the soil porosity by processing addition will be better than blank control, therefore complex microorganism
The addition of microbial inoculum and biological organic complex fertilizer can improve soil texture well, increase the porosity of soil, enhance the suction of soil
Aqueous and gas permeability makes soil more loose.
Downward trend is integrally presented with the increase of drought stress degree in the quantity of edaphon, in composite microbial early period
The addition of object microbial inoculum and biological organic complex fertilizer improves the quantity of edaphon, and the soil in whole cycle well
Micro organism quantity is apparently higher than always blank control.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of composite microbial bacteria, it is characterised in that: composite microbial bacteria is bacillus, saccharomycete and photosynthetic bacteria；Its
In, bacillus, saccharomycete and photosynthetic bacteria press and wait mass ratioes mixing；The bacillus is bacillus subtilis, huge bud
Spore bacillus, bacillus licheniformis, bacillusmusilaginosiengineering, bacillus laterosporus, Paenibacillus polymyxa, liquefying starch gemma bar
One or more of bacterium, Bacillus macerans, bacillus thuringiensis, Bacillus cercus, series bacillus；The yeast
Bacterium is one or more of candida utili, saccharomyces cerevisiae, C. pelliculosa, Candida lipolytica, Saccharomyces paradoxus；Institute
Stating photosynthetic bacteria is one or more of Rhodopseudomonas palustris, colloid Rhodopseudomonas, hydrogenlike silicon ion.
2. composite microbial bacteria according to claim 1, it is characterised in that: the bacterial strain distinguishes fermented and cultured to bacillus
Reach 80% or more with saccharomycete spore count, light and bacterium bacteria concentration reach 1010A/ml or more, then according to isometric ratio
It is mixed.
3. a kind of complex micro organism fungicide, it is characterised in that: complex micro organism fungicide according to parts by weight, described in claim 1
4-10 parts of bacterium solution；80-95 parts of adsorbent；5-15 parts of protective agent.
4. complex micro organism fungicide according to claim 3, it is characterised in that: the complex micro organism fungicide is in parts by weight
Meter, described bacterium solution 6-10 parts；85-95 parts of adsorbent；8-13 parts of protective agent.
5. complex micro organism fungicide according to claim 3, it is characterised in that: the adsorbent is wheat cake powder, wheat bran, bacterium
One or more of slag, bentonite, rotten mosses ash, flyash；The protective agent is starch, vitamin C, lactose, molasses, soybean
One or more of albumen.
6. a kind of preparation method of complex micro organism fungicide as claimed in claim 3, it is characterised in that: according to aforementioned proportion by bacterium
Liquid, adsorbent and protective agent mixing, stir evenly；Powder processed is dried, viable count is effective and reaches 2-9 × 109It is a/gram to get
Microbial inoculum.
7. a kind of biological organic complex fertilizer, which is characterized in that be made of following raw material (weight): described in claim 1
4-10 parts of bacterium solution；60-85 parts of organic matter；5-15 parts of inorganic matter.
8. pressing claim 7 biological organic complex fertilizer, which is characterized in that be made of following raw material (weight): right is wanted
Ask 1 described bacterium solution 6-10 parts；70-80 parts of organic matter；7-10 parts of inorganic matter.
9. pressing claim 8 biological organic complex fertilizer, which is characterized in that the organic matter is feces of livestock and poultry, stalk, wheat cake, bacterium
One or more of slag, vinasse.
The inorganic matter be potassium sulfate, phosphamide zinc, calcium chloride, boron mud, calcium molybdate, containing one of manganese superphosphate or several
Kind.
10. a kind of preparation of biological organic complex fertilizer according to any one of claims 8, which is characterized in that 1) mix organic matter through heap
Fertilizer it is decomposed after sterilize, then according to aforementioned proportion will it is decomposed after organic matter and inorganic matter mixing, dried after mixing well, powder
Broken, it is spare that fineness reaches 60-80 mesh；
2) bacterium solution described in claim 1 is added on step 1) preliminary mixture and is mixed well, be sent into blender stirring；Wait stir
After mixing uniformly, it is re-fed at 30-60 DEG C of roller pelletizer and is granulated to get compound fertilizer.
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