CN109824433A - It is a kind of prevent and treat Strawberry anthracnose microbial organic fertilizer and application - Google Patents

Abstract

The invention belongs to microbial pesticide technical fields, and not ideal enough be also not sufficiently stable of the prevention and treatment to solve the problem of Strawberry anthracnose is unable to satisfy actual demand, provide it is a kind of prevent and treat Strawberry anthracnose microbial organic fertilizer and application.It is prepared by a certain percentage by the sesame dregs of fat, quinoa stalk, black fruit fructus lycii residue, naked oats stalk, broom corn millet stalk, black jade powder, magnesium sulfate, cane molasses, complex micro organism fungicide, egg powder, polyacrylamide；The complex micro organism fungicide is that bacterium, fungi and actinomyces are mixed in proportion, and wherein bacterium is that Bei Laisi bacillus, A Shi bacillus, ZhangZhou bacillus, bacillus polymyxa and bacillus firmus mix in proportion；Fungi is Trichoderma harzianum；Actinomyces are pale purple brown streptomycete.The ability that strawberry resists anthrax bacteria is improved, strawberry root growth is promoted, the quality and yield of strawberry is mentioned；Increase strawberry stolon breeding coefficient, obtains the stolon seedling of more health, obtain more preferable economic benefit.

Description

It is a kind of prevent and treat Strawberry anthracnose microbial organic fertilizer and application

Technical field

The invention belongs to microbial pesticide technical fields, and in particular to a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose And application.

Background technique

Strawberry is rosaceae Fragaria herbaceos perennial, is a kind of higher fruit of economic value, and fruit contains Protein and vitamin abundant, and soft and succulency, sour and sweet palatability are known as " fruit queen ", preferred by everyone.With city The increase of field demand, strawberry cultivating area is also increasing, and Strawberry Seedlings are needed also to increase accordingly.

Strawberry anthracnose is the main soil-borne disease of Strawberry seedling, is the main reason for survival rate is low after facility strawberry is transplanted One of, serious hindrance is brought to strawberry production, the pathogen of anthracnose is colletotrichumColletotrichumSpp. more Kind fungi, presently mainly colletotrichum gloeosporioides PenzC. fructicolaWith strawberry anthrax-bacilusC. thebromicoiaWith sharp spore charcoal Subcutaneous ulcer bacteriumC. nymphaeae.With going deep into for research, there are also some other kinds of anthrax-bacilus can also cause strawberry anthrax for discovery Disease.Varied for the controlling mode of anthracnose in production, biological control method is due to the unicity of bacterial strain, pathogen The complexity of diversity and crop field environment, control efficiency in production is often not ideal enough to be also not sufficiently stable, and is unable to satisfy reality Border demand, so transformation of the biocontrol agent from single bacterial strain to compound strain is imperative.If microbial bacterial agent addition had It, not only can be with the infringement of prevention and control pathogen in machine fertilizer, moreover it is possible to promote plant growth, improve crop quality and yield, to reduce The usage amount of chemicals reaches the requirement of green agriculture development.

Traditional strawberry seedling-raising technique is to carry out breeding and seedling using stolon, is more crawled in order to allow strawberry to generate Crawl stem seedling not only will carry out meticulous management in the stolon pumping stage, it is also necessary to progress disease (predominantly anthracnose) The sub- seedling of strawberry stolon not only can be improved if a certain amount of Special disease prevention fertilizer can be supplemented before stolon generation in prevention and control Reproductive efficiency, it is important to prevent strawberry from disease occurs in seedling stage, finally obtain healthy and strong Strawberry Seedlings.

Sesame is one of Chinese major oil crops, application value with higher.Its seed oil content is up to 55%, Research confirms that the sesame residue after extracting oil still contains there are many nutriment, including crude protein, crude fibre, a great number of elements, micro- The bioactive substances such as secondary element, carbohydrate have Development volue.

In recent years, quinoa is planted experimentally successfully and is widelyd popularize in China, and forms the more complete quinoa kind of scale Plant processing industry chain.Quinoa seed is Quan Guquan nutrition adequate proteins base-forming food, rich in protein, a variety of amino acid, various Mineral element etc., but after its seed harvest, a large amount of stalk is arbitrarily abandoned or is burned, great environmental pollution is not only caused, The significant wastage of quinoa straw biomass resource is also resulted in simultaneously.If quinoa stalk can be used, not only can be improved The value of waste material also is compliant with environmentally protective and clean manufacturing organic fertilizer development need.

More quil shrubs of black fruit fructus lycii category Solanaceae Lycium, the not still excellent pioneer for checking winds and fixing drifting sand and conserving water and soil Tree species have very high ecological application value in improvement of the ecological environment, and its fruit also has high health care and medicine With value.Currently, seed seedling-raising is one of the method for black fruit fructus lycii breeding, and acquire after seed remaining pulp and pericarp just It can be thrown away, cause to waste.Lycium ruthenicum fruit kind nutritional ingredient rich in can have plant growth well after processing Facilitation.

Naked oats, grass family, Avena annual plant also known as naked oats are oat varieties originating in China.China is basic Based on naked oats (also known as naked oats) production, yield accounts for about 90% or more of oat total output.Broom corn millet, grass family, Panicum one Year sward the second cereal crop of sheet.Broom corn millet seed is faint yellow broomcorn millet；Claim milled glutinous broomcorn millet after mill rice peeling, is commonly called as glutinous millet and wears into face, custom Glutinous millet wheaten food is claimed to use.The two while good harvest, also can accordingly generate a large amount of stalk waste materials as quinoa, and study table Bright, the straw crude and crude fat content of naked oats and broom corn millet are higher, so both stalk fermentations are become into organic fertilizer, Can not only stalk be made to turn waste into wealth, and sufficient nutrition can be provided for plant production.

Black jade powder is that waste is generated in black jade process, equally contains 26 kinds of calcium, magnesium, iron, zinc, selenium etc. beneficial to human body Microelement (content meets national soil quality standard GB19618-1995).After ad hoc fashion is handled, black jade powder Itself microelement can be effectively discharged, so it is proportionally added in organic fertilizer, supplement mineral element can be played Effect, promote plant strain growth.

The sesame dregs of fat, quinoa stalk, black fruit fructus lycii residue, naked oats stalk and broom corn millet stalk add by reasonable fermentation Work adds black jade powder and some other ingredients, is prepared into fertilizer special for organic, not only realizes the recycling of waste, solves Certainly crop straw burning causes the problem of environmental pollution, and the organic fertilizer nutritional ingredient produced is high, improves soil environment, increases Soil fertility provides nutrition abundant for plant growth, improves its quality and yield, enhance economic benefit.

Summary of the invention

The present invention is also not sufficiently stable in order to which the control efficiency for solving current Strawberry anthracnose is not ideal enough, is unable to satisfy reality The problem of demand, provide it is a kind of prevent and treat Strawberry anthracnose microbial organic fertilizer and application.

The present invention is realized by following technical solution: a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose, by weighing as follows The raw material of amount part is made: 25-40 parts of the sesame dregs of fat, 10-20 parts of quinoa stalk, 10-15 parts of black fruit fructus lycii residue, naked oats stalk 10-15 parts, 10-20 parts of broom corn millet stalk, 6-11 parts of black jade powder, 0.5-1 parts of magnesium sulfate, 0.5-1 parts of cane molasses, complex microorganism 5-10 parts of microbial inoculum, 0.2-0.3 parts of egg powder, 2-3 parts of polyacrylamide；The complex micro organism fungicide is bacterium, fungi and puts Line bacterium is uniformly mixed by weight 30%-50%:20%-30%:30%-40% ratio and is made, and wherein bacterium is Bei Laisi bacillus (Bacillus velezensis), A Shi bacillus (Bacillus aryabhattai), ZhangZhou bacillus (Bacillus zhangzhouensis), bacillus polymyxa (Bacillus polymyxa Macé) and bacillus firmus (Bacillus firmus) 5 bacterial strains, it is uniformly mixed by weight 3:2:1:2:1；Fungi be Trichoderma harzianum (Trichoderma harzianum)；Actinomyces be pale purple brown streptomycete (Streptomyces enissocaesilis).

In the complex micro organism fungicide, effective active bacterium number amount >=6 × 10 of bacterium⁹Cfu/g, the bacteria containing amount of fungi >= 6×10⁸Cfu/g, effective active bacterium number amount >=10 of actinomyces⁸cfu/g；Bacterium, fungi and the total bacteria containing amount of actinomyces >= 10⁹cfu/g。

The preparation of micro-ecological complex bacterial agent: bacterium is cultivated respectively with routine LB culture medium, by inclined-plane, seed, liquid state fermentation Third stage culture, 25-30 DEG C of cultivation temperature, incubation time is 36-48 hours；The conventional PDA culture medium culture of fungi, by inclined-plane, Seed, liquid state fermentation third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-72 hours；Actinomyces are trained with routine Gao Shi Base culture is supported, by inclined-plane, seed, liquid state fermentation third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-60 hours；Carefully Bacterium, fungi and actinomyces carry out the culture solution of liquid state fermentation acquisition respectively, mix respectively with wheat bran according to the ratio of 3:1, uniformly It is dried at 40 DEG C after mixing, until water content is controlled in 3-5%, powder is made through pulverizer in obtained solid, finally by 5 kinds of bacterium Agent powder proportionally mixes, and obtains complex micro organism fungicide.

The sesame dregs of fat are that sesame has squeezed the residue after oil, and black fruit fructus lycii residue is the pulp and fruit after having collected seed Skin.

A kind of method of the microbial organic fertilizer of preparation prevention and treatment Strawberry anthracnose, steps are as follows:

(1) stalk is handled: quinoa stalk, naked oats stalk and broom corn millet stalk are crushed to the length of 2-3cm, spare；

(2) material mixes: the sesame dregs of fat, black fruit fructus lycii residue, the quinoa stalk of crushing, naked oats stalk and broom corn millet stalk are added Enter into fermentation vat, it is 45%-50% that the water content of material is made after adding water and stirring, and complex micro organism fungicide 2-5 is then added Part, it stirs evenly；

(3) ferment: mixed material is sealed fermentation and is stirred reduction fermentation temperature when temperature of charge is 60-70 DEG C； After in triplicate, fermentation ends；

(4) prepare ferment particulate: the product after fermentation spontaneously dries, and after water content is 3-5%, being ground into granularity is 3-5mm's Particle；

(5) it prepares powder: black jade powder, magnesium sulfate, cane molasses and remaining complex micro organism fungicide is sufficiently mixed in proportion, 70-75 DEG C of heat preservation 20min, it is spare；

(6) above-mentioned powder, polyacrylamide and egg powder mixing is added in ferment particulate, is sent into Squeezinggranulator, 1-2mm is made Granulated fertilizer.

It is involved in the present invention to bacterial strain can be commercially available on the market.

When material mixes moisture properly whether judge method: hand grabs a material, and water breakthrough print is not dripped, and landing can scatter but It can.

Bei Laisi bacillus (B .velezensis), bacillus polymyxa (B. polymyxaMac é) and strong bud Spore bacillus (B.firmus) secondary metabolites such as her withered grass element can be secreted during the growth process, it will lead to anthrax bacteria mycelia not Can normal growth, expand, branch falls off and the abnormal phenomenon such as many places fracture, while spore germination also will receive inhibition； A Shi bacillus (Bacillus aryabhattai) and ZhangZhou bacillus (B. zhangzhouensis) some lifes of secretion The growth-promotings substance such as long element promotes the pumping of strawberry root growth and stolon, enhances the absorbability of plant, promotes plant strong Kang Chengchang.Pale purple brown streptomycete (S.enissocaesilis) generate streptomysin and polysaccharide antibiotic, can effectively inhibit The growth and breeding for opportunistic pathogen of causing a disease.Trichoderma harzianum (T. harzianum) can secretase and antibiotic agents, destroy fungal cell Wall construction causes pathogen dead；And the Trichoderma harzianum speed of growth is quickly, and quick breeding in the soil can seize plant The site in root system body surface face forms a separation net, to reduce the machine of pathogen infection root system between pathogen and root system Rate.

Advantageous effects of the invention: the present invention uses the black fruit extracted the sesame dregs of fat for having squeezed oil with collected seed Fructus lycii residue, quinoa stalk, naked oats stalk, broom corn millet stalk and black jade powder etc. are used as primary raw material, using composite microbial bacteria Agent is fermented, and is prepared the dedicated fertilizer of the anti-anthracnose of strawberry, is adequately achieved the utilization again of waste, improve waste material Value also is compliant with environmentally protective and clean manufacturing organic fertilizer development need.

In addition, because still containing a large amount of crude protein, crude fibre, amino acid, a great number of elements, micro member in fermentation materials Element, alkaloid, polysaccharide isoreactivity substance, the organic principle in the organic fertilizer of production is higher, improves soil fertility, enhances soil Organic matter has sufficiently ensured the nutritional need of strawberry growth especially stolon pumping phase；Meanwhile in fertilizer be added diseases prevention and The microorganism of growth-promoting have multiple benefits, first, can be improved strawberry resist anthrax bacteria ability, exempt from it in seedling stage In the infringement by pathogen；Second, strawberry root growth can be promoted, mention the quality and yield of strawberry；Third, The breeding coefficient that strawberry stolon can be increased obtains more healthy stolon seedlings, to obtain more preferable economic benefit.

Detailed description of the invention

Fig. 1 is biocontrol microorganisms to the inhibitory effect of strawberry anthrax-bacilus, in figure: A: Bei Laisi bacillus；B: mostly viscous gemma bar Bacterium；C: bacillus firmus；D: pale purple brown streptomycete；CK: strawberry anthrax-bacilus；Fig. 2 is suppression of the Trichoderma harzianum to strawberry anthrax-bacilus Effect processed, in figure: a is inhibitory effect figure；B is pathogen comparative diagram；Fig. 3 is complex micro organism fungicide for Strawberry anthracnose Greenhouse prevents and treats hillslope processes and divides schematic diagram, in figure: CK indicates that control group, D indicate processing group.

Specific embodiment

Embodiment 1: bacteriostatic experiment of the bacterial strain for anthracnose

Experimental method: use tablet face-off method, PDA plate center inoculation diameter be 8mm strawberry anthrax-bacilus (C. thebromicoia), away from 3 cm of fungus block be inoculated with Bei Laisi bacillus (B. velezensis), bacillus polymyxa (B.polymyxa Macé), bacillus firmus (B. firmus), Trichoderma harzianum (T. harzianum) and pale purple brown strepto- Bacterium (S. enissocaesilis), it compares to be only inoculated with the plate of strawberry anthrax-bacilus, 28 DEG C of culture 3-5 d are repeated 3 times, and are seen Examine experimental result.

Bacteriostatic experiment the result is shown in Figure 1-2.It is seen in fig. 1, that 3 plants of bacteriums and 1 streptomycete, which all have, inhibits strawberry anthrax The ability of bacterium growth, fungistatic effect are fairly obvious.From figure 2, it is seen that Trichoderma harzianum is able to suppress the mycelia growth of strawberry anthrax-bacilus, It stimulates it to enter dormant state, generates spore；Simultaneously it has also been found that the growth of trichoderma is very rapid, it was confirmed that the energy of its " robbing position " Power.

Embodiment 2: the ability of bacterial strain production IAA

Experimental method: strain growth element IAA secretion capacity is measured with Salkowski method.By bacterial strain A Shi bacillus (B. aryabhattai) and ZhangZhou bacillus (B. zhangzhouensis) be inoculated into LB culture medium respectively (50ml), 28 DEG C 150 rmin-1 cultivate 36h-48h, collect fermentation liquid, and 8000 rmin-1 are centrifuged 2 min, thallus are removed, in collection Clear filtrate, it is spare.

Qualitative detection: 15 min color changes are stored at room temperature as judgment basis to be added after color solution, determine that bacterial strain is The no ability with producing IAA；Quantitative detection: resulting supernatant adds the color solution of diploid product, room after bacteria suspension centrifugation Warm dark place is developed the color after 30 min, and OD value is measured at 530 nm, calculates strain to be tested referring to auxin standard curve Auxin yield, the results are shown in Table 1.

From 1 interpretation of result of table, find A Shi bacillus (B. aryabhattai) and ZhangZhou bacillus (B.zhangzhouensis) producing IAA ability it is very significant, both illustrate there are the potentiality for promoting plant growth.

The measurement of auxin secretion capacity in 1 bacterial strain fermentation liquor of table

Bacterial strain	Produce IAA/ (μ gmL-1)
		A Shi bacillus (Bacillus aryabhattai)	6.58±0.14
ZhangZhou bacillus (Bacillus zhangzhouensis)	6.00±0.12

Embodiment 3: complex micro organism fungicide tests the prevention and treatment of Strawberry anthracnose

This experiment prepares organic fertilizer by taking strawberry " beauty " as an example.

According to the growth conditions of planting strawberry, the composition of organic fertilizer are as follows: 32 parts of the sesame dregs of fat, 15 parts of quinoa stalk, black fruit 10 parts of fructus lycii residue, 15 parts of broom corn millet stalk, 7 parts of black jade powder, 1 part of magnesium sulfate, 0.8 part of cane molasses, is answered at 10 parts of naked oats stalk Close 7 parts of microbial bacterial agent (using in two times), 0.2 part of egg powder, 2 parts of polyacrylamides.

The sesame dregs of fat are that sesame has squeezed the residue after oil, and black fruit fructus lycii residue is the pulp and fruit after having collected seed Skin.

Complex micro organism fungicide is that bacterium, fungi and actinomyces are made by weight 50%:20%:30% ratio is uniformly mixed, Wherein bacterium include Bei Laisi bacillus (B.velezensis), A Shi bacillus (B.aryabhattai) and ZhangZhou bud Spore bacillus (B.zhangzhouensis) and bacillus polymyxa (B.polymyxa Macé), bacillus firmus (B.firmus) 5 bacterial strains, it is uniformly mixed by weight 3:2:1:2:1；Fungi be Trichoderma harzianum (T.harzianum)；Unwrapping wire Bacterium be pale purple brown streptomycete (S.enissocaesilis).In the complex micro organism fungicide, the effective active bacterium number amount of bacterium ≥6×10⁹Cfu/g, bacteria containing amount >=6 × 10 of fungi⁸Cfu/g, effective active bacterium number amount >=10 of actinomyces⁸cfu/g；Bacterium, Fungi and total bacteria containing amount >=10 of actinomyces⁹cfu/g。

The preparation of micro-ecological complex bacterial agent: bacterium is cultivated respectively with routine LB culture medium, by inclined-plane, seed, liquid state fermentation Third stage culture, 25-30 DEG C of cultivation temperature, incubation time is 36-48 hours；The conventional PDA culture medium culture of fungi, by inclined-plane, Seed, liquid state fermentation third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-72 hours；Actinomyces are trained with routine Gao Shi Base culture is supported, by inclined-plane, seed, liquid state fermentation third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-60 hours；Carefully Bacterium, fungi and actinomyces carry out the culture solution of liquid state fermentation acquisition respectively, mix respectively with wheat bran according to the ratio of 3:1, uniformly It is dried at 40 DEG C after mixing, until water content is controlled in 3-5%, powder is made through pulverizer in obtained solid, finally by 5 kinds of bacterium Agent powder proportionally mixes, and obtains complex micro organism fungicide.

Prevent and treat microbial organic fertilizer specific of Strawberry anthracnose the preparation method comprises the following steps:

(1) quinoa stalk, naked oats stalk and broom corn millet stalk are crushed to the length of 2-3cm, spare.

(2) by 32 parts of the sesame dregs of fat, 10 parts of black fruit fructus lycii residue, 15 parts of the quinoa stalk crushed, 10 parts of naked oats stalk It is added in fermentation vat with 15 parts of broom corn millet stalk, it is 45%-50% that the water content of material is made after adding water and stirring, and is then added multiple 3 parts of microbial bacterial agent are closed, is stirred evenly.Moisture properly whether judge method: hand grabs a material, and water breakthrough print is not dripped, and lands It can scatter.

(3) above-mentioned substance is sealed fermentation, detects the temperature inside material at any time, when temperature rises to 60-70 DEG C, Second is carried out to stir；After repeating three times in this way, fermentation process terminates substantially.

(4) product after fermenting spontaneously dries, and after water content is 3-5%, is ground into the particle that granularity is 3-5mm.

(5) powder is made.By 7 parts of black jade powder, 1 part of magnesium sulfate, 0.8 part of cane molasses and 4 parts of complex micro organism fungicide, fill Point mixing, 70-75 DEG C of heat preservation 20min are spare.

(6) above-mentioned powder, 2 parts of polyacrylamide and 0.2 part of egg powder mixing is added in ferment particulate, is sent into Squeezinggranulator In, the granulated fertilizer of 1-2mm is made.

Experimental example 1: potting control efficiency experiment

The microbial organic fertilizer of the prevention and treatment Strawberry anthracnose prepared is added in sterilization matrix by 7d before transplanting；Choose health , the healthy Strawberry seedlings of consistent 2 leaf, 1 heart of growth conditions are transplanted；10 d carry out hurting root processing after Strawberry Seedlings field planting, together When pour strawberry anthrax-bacilus spore suspension dilution (concentration reaches 1 × 10⁶ Cfu/mL).

4 processing of test setting: (1) C1: pathogen；(2) C2: pathogen+complex microorganism organic fertilizer；(3) C3: prevention and treatment The microbial organic fertilizer of Strawberry anthracnose；(4) (fertilizer is not added) in CK, control group.30 plants of Strawberry Seedlings of every processing kind, are repeated 3 times, conventional Management observes Strawberry Seedlings incidence after being inoculated with 50 d, while the plant height, blade area, root fresh weight and the cauline leaf that measure strawberry are fresh Weight.It the results are shown in Table 2.

Control efficiency and growth-promoting effect of 2 complex microorganism organic fertilizer of table to Strawberry anthracnose

Disease index

Control efficiency (%)

Plant height (cm)

Root fresh weight (g)

Root dry weight (g)

Cauline leaf fresh weight (g)

Cauline leaf dry weight (g)

C1

62.00±0.02

17.74±1.07d

30.98±3.00c

12.39±1.20c

24.65±2.90d

5.67±0.67d

C2

20.17±0.03

70.69

24.92±1.97c

69.33±5.44b

17.33±1.36b

52.56±3.39c

9.46±0.61c

C3

31.21±1.84a

76.55±2.45a

19.14±0.61a

65.17±2.29a

11.73±0.41a

CK

27.57±0.36b

71.22±3.99b

17.80±1.00b

55.46±3.52b

9.98±0.63b

For serious analysis table 2 as a result, by finding after the data comparison of C1 and C2, complex microorganism organic fertilizer being capable of effectively preventing Infringement of the Strawberry anthracnose to plant, after application, disease index is reduced to 20.17 by 62, and control efficiency is up to 70.69%；And And while controlling disease, complex microorganism organic fertilizer further promotes the growth of strawberry, plant height, root fresh weight, root dry weight, Cauline leaf fresh weight and cauline leaf dry weight improve 40.47%, 123.79%, 39.87%, 112.22% and 66.84% than C1 group respectively.

C3 is compared with CK illustrates strawberry when not by invading pathogens, and complex microorganism organic fertilizer is raw for strawberry Long facilitation effect.As a result, it has been found that complex microorganism organic fertilizer has effectively promoted strawberry really in potted plant experiment Growth, indices are higher by than control group very much, and plant height, root fresh weight, root dry weight, cauline leaf fresh weight and cauline leaf dry weight mention respectively It is high by 13.20%, 7.50%, 7.53%, 17.51% and 25.07%.

In conclusion the comparing result of two groups of potted plant experiments absolutely proved complex microorganism organic fertilizer in strawberry growth-promoting and Diseases prevention has good effect.

Experimental example 2: complex micro organism fungicide tests (greenhouse experiment) for the prevention and treatment of Strawberry anthracnose

In greenhouse, the long 15m in strawberry cultivating ridge, row spacing 0.45m, spacing 1.0m, the two rows of plantations of strawberry point are on ridge, line-spacing For 25cm, spacing in the rows 15cm.The processing of soil is carried out before transplanting Strawberry Seedlings.

Experimental setup 2 processing: (1) control group: 50kg/ mus of composite chemical fertilizer usage amount, 40kg/ mus of calcium superphosphate；In grass Certain kind of berries growth period can be top dressing 1-2 times, apply compound fertilizer 5-8kg/ mus every time；(2) processing group :+50% chemical fertilizer of complex microorganism organic fertilizer, 50kg/ mus of microorganism fertilizer amount of application, usage amount 25kg/ mus of compound fertilizer, 20kg/ mus of calcium superphosphate；Microorganism fertilizer is applied when top dressing 6-8kg/ mus of amount, usage amount 2-4kg/ mus of compound fertilizer.Every group of 3 repetitions, totally 6 cells are (see Fig. 3；Each cell 5m*4.5m), Each cell plants 68 plants of strawberries, chooses 10 plants of strawberries and is listed, routine observation.

Incidence is observed continuously in strawberry growth period, and in plant height, blade area, the root fresh weight of florescence measurement strawberry With cauline leaf fresh weight, fruit yield statistics is carried out in fruiting period.Experimental result is shown in Table 3.

3 complex microorganism organic fertilizer of table is to strawberry growth promoting function

Plant height (cm)

Root fresh weight (g)

Cauline leaf fresh weight (g)

Soluble solid (%)

Mean fruit weight (g/)

Single plant yield (g/ plants)

Control group

30.33±0.40b

78.33±4.39b

61.00±3.87b

8.89±0.28b

21.06±1.93b

283.5±5.36b

Processing group

35.89±2.12a

84.21±2.69a

71.69±2.52a

12.17±0.30a

32.05±1.03a

326.4±4.56a

From table 3 it can be seen that strawberry is grown complex microorganism organic fertilizer and fruit product in greenhouse experiment The facilitation effect of matter and yield is clearly.Strawberry improves 18.33%, and root fresh weight increases 7.51%, and cauline leaf fresh weight mentions It is high by 17.52%, it is more obvious than the growth-promoting better effect of potted plant experiment.

The soluble solid of strawberry fruit also increases 36.90%, and mean fruit weight improves 52.18%, single plant strawberry Yield increase 15.13%, quality and yield is all significantly improved.

Embodiment 4: stolon Biological control

The preparation for preventing and treating the microbial organic fertilizer of Strawberry anthracnose, according to the growth conditions of planting strawberry, the composition of organic fertilizer are as follows: 40 parts of the sesame dregs of fat, 10 parts of quinoa stalk, 12 parts of black fruit fructus lycii residue, 12 parts of naked oats stalk, 10 parts of broom corn millet stalk, black jade powder 6 parts, 0.7 part of magnesium sulfate, 0.5 part of cane molasses, 10 parts of complex micro organism fungicide (using in two times), it is 0.25 part of egg powder, poly- 2.5 parts of acrylamide.The sesame dregs of fat are that sesame has squeezed the residue after oil, and black fruit fructus lycii residue is the pulp collected after seed And pericarp.

The complex micro organism fungicide is that bacterium, fungi and actinomyces are uniformly mixed by weight 40%:25%:35% ratio Be made, wherein bacterium be Bei Laisi bacillus (B.velezensis), A Shi bacillus (B.aryabhattai), ZhangZhou Bacillus (B.zhangzhouensis), bacillus polymyxa (B.polymyxa Macé) and bacillus firmus (B. firmus) 5 bacterial strains, it is uniformly mixed by weight 3:2:1:2:1；Fungi be Trichoderma harzianum (T.harzianum)；Actinomyces are Pale purple brown streptomycete (S. enissocaesilis).

The specific of the microbial organic fertilizer of Strawberry anthracnose is prevented and treated the preparation method comprises the following steps: being then added when material mixes compound micro- It 5 parts of bacteria agent, stirs evenly；By black jade powder, magnesium sulfate, cane molasses and remaining complex micro organism fungicide 5 when preparing powder It part is sufficiently mixed, 70-75 DEG C of heat preservation 20min is spare.Remaining preparation method is the same as preparation method described in embodiment 3.

The experiment of stolon pumping is carried out to strawberry in greenhouse, two groups are arranged altogether: control group and experimental group, every group 10 plants grow The strawberry of state consistency.

Control group applies fertilizer according to Routine Management；Experimental group supplement on the basis of normal fertilising applies the embodiment of the present invention Fertilizer special for organic, supplemented before stolon pumping, every plant of strawberry 50g fertilizer, then according to circumstances supplement 2-3 times.Except this Except, experimental group is identical with the field management measure of control group.After stolon is grown, statistical result.

The result shows that strawberry stolon pumping item number can be made to increase using fertilizer special for organic of the invention, every is crawled Stem number of seedling increases, and final experimental group is total to 356 plants of seedling, and control group only has 318 plants, and number of seedling increases by 14.78%, and strawberry crawls The supplement of stem special fertilizer improves the breeding coefficient of nursery.

Influence of 4 complex microorganism organic fertilizer of table to strawberry stolon pumping

	Strawberry Seedlings number	Final number of seedling	Breeding coefficient increase rate
				Control group	10	318	-
Experimental group	10	356	14.78%

Embodiment 5: preventing and treating the preparation of the microbial organic fertilizer of Strawberry anthracnose, according to the growth conditions of planting strawberry, organic fertilizer Composition are as follows: 25 parts of the sesame dregs of fat, 20 parts of quinoa stalk, 15 parts of black fruit fructus lycii residue, 15 parts of naked oats stalk, broom corn millet stalk 20 Part, 11 parts of black jade powder, 0.5 part of magnesium sulfate, 1 part of cane molasses, 5 parts of complex micro organism fungicide (using in two times), egg powder 0.3 Part, 3 parts of polyacrylamide.The sesame dregs of fat are that sesame has squeezed the residue after oil, and black fruit fructus lycii residue is after having collected seed Pulp and pericarp.

The complex micro organism fungicide is that bacterium, fungi and actinomyces are uniformly mixed by weight 30%:30%:40% ratio Be made, wherein bacterium be Bei Laisi bacillus (B. velezensis), A Shi bacillus (B.aryabhattai), ZhangZhou Bacillus (B.zhangzhouensis), bacillus polymyxa (B.polymyxa Macé) and bacillus firmus (B. firmus) 5 bacterial strains, it is uniformly mixed by weight 3:2:1:2:1；Fungi be Trichoderma harzianum (T. harzianum)；Actinomyces For pale purple brown streptomycete (S. enissocaesilis).

The specific of the microbial organic fertilizer of Strawberry anthracnose is prevented and treated the preparation method comprises the following steps: being then added when material mixes compound micro- It 2 parts of bacteria agent, stirs evenly；By black jade powder, magnesium sulfate, cane molasses and remaining complex micro organism fungicide 3 when preparing powder It part is sufficiently mixed, 70-75 DEG C of heat preservation 20min is spare.Remaining preparation method is the same as preparation method described in embodiment 3.

Claims (5)

1. a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose, it is characterised in that: be made of the raw material of following parts by weight: sesame 25-40 parts of the dregs of fat, 10-20 parts of quinoa stalk, 10-15 parts of black fruit fructus lycii residue, 10-15 parts of naked oats stalk, broom corn millet stalk 10- 20 parts, 6-11 parts of black jade powder, 0.5-1 parts of magnesium sulfate, 0.5-1 parts of cane molasses, 5-10 parts of complex micro organism fungicide, egg powder 0.2-0.3 parts, 2-3 parts of polyacrylamide；The complex micro organism fungicide is bacterium, fungi and actinomyces by weight 30%- 50%:20%-30%:30%-40% ratio is uniformly mixed and is made, and wherein bacterium is Bei Laisi bacillus (Bacillus Velezensis), A Shi bacillus (Bacillus aryabhattai), ZhangZhou bacillus (Bacillus Zhangzhouensis), bacillus polymyxa (Bacillus polymyxa Mac é) and bacillus firmus (Bacillus Firmus) 5 bacterial strains are uniformly mixed by weight 3:2:1:2:1；Fungi is Trichoderma harzianum (Trichoderma Harzianum)；Actinomyces are pale purple brown streptomycete (Streptomyces enissocaesilis).

2. a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose according to claim 1, it is characterised in that: described compound In microbial bacterial agent, effective active bacterium number amount >=6 × 10 of bacterium⁹Cfu/g, bacteria containing amount >=6 × 10 of fungi⁸Cfu/g, unwrapping wire Effective active bacterium number amount >=10 of bacterium⁸cfu/g；Bacterium, fungi and total bacteria containing amount >=10 of actinomyces⁹cfu/g。

3. a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose according to claim 1, it is characterised in that: micro- life The preparation of state composite bacteria agent: bacterium is cultivated respectively with routine LB culture medium, by inclined-plane, seed, liquid state fermentation third stage culture, culture 25-30 DEG C of temperature, incubation time is 36-48 hours；The conventional PDA culture medium culture of fungi, by inclined-plane, seed, liquid state fermentation Third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-72 hours；Actinomyces routine Gao Shi culture medium culture, by oblique Face, seed, liquid state fermentation third stage culture, 28-32 DEG C of cultivation temperature, incubation time is 48-60 hours；Bacterium, fungi and unwrapping wire Bacterium carries out the culture solution of liquid state fermentation acquisition respectively, mixes respectively with wheat bran according to the ratio of 3:1, dries after evenly mixing in 40 DEG C Dry, until water content is controlled in 3-5%, powder is made through pulverizer in obtained solid, finally proportionally mixes 5 kinds of microbial inoculum powder It closes, obtains complex micro organism fungicide.

4. a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose according to claim 1, it is characterised in that: the sesame The dregs of fat are that sesame has squeezed the residue after oil, and black fruit fructus lycii residue is the pulp and pericarp collected after seed.

5. the method for preparing a kind of microbial organic fertilizer for preventing and treating Strawberry anthracnose of any of claims 1 or 2, feature exist In: steps are as follows:

(1) stalk is handled: quinoa stalk, naked oats stalk and broom corn millet stalk are crushed to the length of 2-3cm, spare；

(2) material mixes: the sesame dregs of fat, black fruit fructus lycii residue, the quinoa stalk of crushing, naked oats stalk and broom corn millet stalk are added Enter into fermentation vat, it is 45%-50% that the water content of material is made after adding water and stirring, and complex micro organism fungicide 2-5 is then added Part, it stirs evenly；

(3) ferment: mixed material is sealed fermentation and is stirred reduction fermentation temperature when temperature of charge is 60-70 DEG C； After in triplicate, fermentation ends；

(4) prepare ferment particulate: the product after fermentation spontaneously dries, and after water content is 3-5%, being ground into granularity is 3-5mm's Particle；

(5) it prepares powder: black jade powder, magnesium sulfate, cane molasses and remaining complex micro organism fungicide is sufficiently mixed in proportion, 70-75 DEG C of heat preservation 20min, it is spare；

(6) above-mentioned powder, polyacrylamide and egg powder mixing is added in ferment particulate, is sent into Squeezinggranulator, 1-2mm is made Granulated fertilizer.