CN107723258A - Prevent and treat composite bacteria agent of tobacco bacterial wilt and preparation method thereof - Google Patents
Prevent and treat composite bacteria agent of tobacco bacterial wilt and preparation method thereof Download PDFInfo
- Publication number
- CN107723258A CN107723258A CN201710943241.3A CN201710943241A CN107723258A CN 107723258 A CN107723258 A CN 107723258A CN 201710943241 A CN201710943241 A CN 201710943241A CN 107723258 A CN107723258 A CN 107723258A
- Authority
- CN
- China
- Prior art keywords
- composite bacteria
- bacteria agent
- seed liquor
- bacillus
- fermentation medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agronomy & Crop Science (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to composite bacteria agent preparation field, more particularly to a kind of composite bacteria agent for preventing and treating tobacco bacterial wilt and preparation method thereof, comprise the following steps：Inclined-plane culture is carried out to original strain, seed culture obtains seed liquor, fermentation medium is prepared, seed liquor is mixed in the fermentation medium, obtains liquid composite bacteria agent, eight kinds of microorganisms are mixed bacterium compound criteria by the composite bacteria agent of the preventing and treating tobacco bacterial wilt with tank, mutually antagonism, alternate do not coexist each bacterial strain, and the effect for preventing and treating tobacco bacterial wilt is good, it is environmentally safe, and the production to tobacco seedlings has stimulation, the economical character of tobacco seedlings is significantly improved, is advantageous to improve yield of tobacco.
Description
Technical field
The present invention relates to composite bacteria agent preparation field, more particularly to a kind of composite bacteria agent and its system for preventing and treating tobacco bacterial wilt
Preparation Method.
Background technology
Tobacco bacterial wilt is a kind of worldwide great disease caused by eggplant section thunder Salmonella.The disease is also known as cigarette pest, is typical case
Vascular bundle diseases.Occur in recent years in south China cigarette district heavier, the cigarette strain incidence of disease is up to 20% -30% when serious；Tobacco grower commonly uses
Agricultural streptomycin solution pours cigarette strain, can put off the morbidity of bacterial wilt, but long-term use of, disease microorganism can be caused to produce anti-
Medicine reacts, and causes secondary pollution, and preventive effect is undesirable.
The content of the invention
It is an object of the invention to the pharmacy control efficacy for overcoming current treatment tobacco bacterial wilt is undesirable, and make for a long time
Reacted with anti-medicine is produced, the problem of causing secondary pollution, and a kind of composite bacteria agent for preventing and treating tobacco bacterial wilt and its preparation are provided
Eight kinds of microorganisms are mixed bacterium compound criteria by method, the composite bacteria agent of the preventing and treating tobacco bacterial wilt with tank, the mutual not antagonism of each bacterial strain, mutually
Life coexists, and it is good to prevent and treat the effect of tobacco bacterial wilt, environmentally safe, and the production to tobacco seedlings has stimulation, significantly
Ground improves the economical character of tobacco seedlings, is advantageous to improve yield of tobacco.
What the present invention was realized in：A kind of composite bacteria agent for preventing and treating tobacco bacterial wilt, by seed liquor and with tank fermentation
Fermentation medium is made, and the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 22-28%, more glutinous bud
Spore bacillus 2-8%, side spore bacillus brevis 12-18%, colloid bacillus cereus 22-28%, Trichoderma harzianum 2-8%, Trichoderma viride 2-8%,
Photosynthetic bacteria 12-18% and pythium oligandrum 2-8%；The fermentation medium consists of the following components in percentage by weight：Molasses 1.2-
1.4%th, glucose 0.7-0.9%, cornstarch 1.5-1.7%, bean cake powder 1.4-1.6%, magnesium sulfate 0.02-0.04%, pyroligneous acid
1.4-1.6%, urea 0.11-0.13%, potassium dihydrogen phosphate 0.03-0.05%, peptone 0.09-0.11%, dusty yeast 0.04-
0.06% and the water of surplus.
The composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, it is made by seed liquor and with the fermentation medium of tank fermentation, institute
Seed liquor is stated to be made up of the strain of following percentage by weight：Bacillus subtilis 25%, Bacillus polymyxa 5%, the short brood cell of side spore
Bacillus 15%, colloid bacillus cereus 25%, Trichoderma harzianum 5%, Trichoderma viride 5%, photosynthetic bacteria 15% and pythium oligandrum 5%；The fermentation
Culture medium comprises the following raw materials by weight percent：Molasses 1.2%, glucose 0.8%, cornstarch 1.6%, bean cake powder 1.5%,
Magnesium sulfate 0.03%, pyroligneous acid 1.5%, urea 0.12%, potassium dihydrogen phosphate 0.04%, peptone 0.1%, dusty yeast 0.05% and surplus
Water.
A kind of preparation method of the composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, comprises the following steps：
Step 1：Inclined-plane culture is carried out to original strain：By bacillus subtilis, Bacillus polymyxa, side spore bacillus brevis,
Colloid bacillus cereus is inoculated on beef extract-peptone agar medium respectively, is cultivated 2-3 days at 28-32 DEG C, will breathe out thatch wood
Mould, pythium oligandrum, Trichoderma viride are inoculated on PDA- glucose agar mediums respectively, are cultivated 3-4 days at 28-30 DEG C, will
Photosynthetic bacteria is inoculated on beef extract-peptone agar medium, is cultivated 3-5 days at 32-35 DEG C；
Step 2：The strain obtained to step 1 carries out seed culture：Bacillus subtilis that step 1 is obtained, more glutinous gemma bar
Bacterium, side spore bacillus brevis, colloid bacillus cereus are inoculated into beef extract-peptone liquid triangular flask culture medium respectively, in 28-
Cultivated 2-4 days at 32 DEG C, the Trichoderma harzianum that step 1 is obtained, pythium oligandrum, Trichoderma viride are inoculated into PDA- Glucose Liquids respectively
In body triangular flask culture medium, cultivated 3-4 days at 28-30 DEG C, the photosynthetic bacteria that step 1 is obtained is inoculated into the special liquid of photosynthetic bacteria
In body triangular flask culture medium, cultivated 3-7 days at 32-35 DEG C；
Step 3：Prepare seed liquor：The strain that step 2 obtains is weighed according to following percetage by weight：Bacillus subtilis 22-
28%th, Bacillus polymyxa 2-8%, side spore bacillus brevis 12-18%, colloid bacillus cereus 22-28%, Trichoderma harzianum 2-8%, green
Color trichoderma 2-8%, photosynthetic bacteria 12-18%, pythium oligandrum 2-8%, seed liquor is obtained after well mixed；
Step 4：The seed liquor that step 3 obtains is mixed：Comprise the following steps：
A:Produce fermentation medium：By fermentation tank, drying and sterilizing handles 30-50min at 80-100 DEG C, then according to following heavy
Amount percentage inserts raw material into fermentation tank：Molasses 1.2-1.4%, glucose 0.7-0.9%, cornstarch 1.5-1.7%, dregs of beans
Powder 1.4-1.6%, magnesium sulfate 0.02-0.04%, pyroligneous acid 1.4-1.6%, urea 0.11-0.13%, potassium dihydrogen phosphate 0.03-
0.05%th, peptone 0.09-0.11%, dusty yeast 0.04-0.06% and surplus water, then instilled into fermentation tank and account for fermented and cultured
Base cumulative volume 0.05-0.07% microelement nutritious liquid, sterilization treatment is carried out to fermentation medium under 110-130 DEG C of environment
30-90min, fermentation medium is finally cooled to 25-35 DEG C, it is standby；
B：It is inoculated with seed liquor：The seed liquor that inoculation step 3 obtains in the fermentation medium obtained by step A, keeping temperature 25-35
DEG C, rotating speed 160-200r/min, while according to seed liquor：The volume ratio of air is 1:0.8 ratio is passed through pure air, docking
Fermentation medium after kind carries out aerobic culture 24-36 hours, and it is 1800-2200LX to control illumination, obtains liquid composite bacteria agent；
Step 5：Prepare solid powder：Raw material is weighed according to following percetage by weight：In the liquid composite bacteria agent obtained to step 4
Solid matrix is added, according to liquid composite bacteria agent：Solid matrix is 1：0.8 ratio stirs and evenly mixs, and is then put into mixture
Hothouse, which is dried to moisture, is less than 8%, then is crushed to particle diameter and reaches below 1mm, obtains solid, powdery composite bacteria agent.
The preparation method of the composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, the solid matrix are turf powder, rice bran, height
One or more in ridge soil, sterilizing organic fertilizer, humic acid, white carbon.
The present invention has the following advantages that：Composite bacteria agent of preventing and treating tobacco bacterial wilt of the present invention and preparation method thereof, will
Eight kinds of microorganisms mix bacterium compound criteria with tank, and mutually antagonism, alternate do not coexist each bacterial strain, complement each other, product bacteria containing amount is high, the bacterium
Agent energy evoking tobacco crop produces Sudismase, when tobacco is by adverse circumstances such as disease, insect pest, arid, agings, produces freely
Base mitigates disease to improve the resistance of tobacco, makes it have the effect of preventing and treating tobacco bacterial wilt well, and the microbial inoculum pair
The production of tobacco seedlings has stimulation, significantly improves the economical character of tobacco seedlings, be advantageous to improve yield of tobacco, the microbial inoculum without
Poison is harmless, to environment without not contaminating after use, ensure that Environmental security environmental protection, is advantageous to the green production of tobacco.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1：A kind of composite bacteria agent for preventing and treating tobacco bacterial wilt, by seed liquor and the fermentation medium system with tank fermentation
Into the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 24%, Bacillus polymyxa 4%, side spore are short
Bacillus 14%, colloid bacillus cereus 26%, Trichoderma harzianum 6%, Trichoderma viride 6%, photosynthetic bacteria 16% and pythium oligandrum 4%, it is described
Fermentation medium consists of the following components in percentage by weight：Molasses 1.2%, glucose 0.7%, cornstarch 1.5%, bean cake powder
1.4%th, magnesium sulfate 0.02%, pyroligneous acid 1.4%, urea 0.11%, potassium dihydrogen phosphate 0.03%, peptone 0.09%, dusty yeast 0.04%
With the water of surplus；
A kind of preparation method of the composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, comprises the following steps：
Step 1：Inclined-plane culture is carried out to original strain：By bacillus subtilis, Bacillus polymyxa, side spore bacillus brevis,
Colloid bacillus cereus is inoculated on beef extract-peptone agar medium respectively, is cultivated 2 days at 28 DEG C, by Trichoderma harzianum, widow
Male rotten mould, Trichoderma viride is inoculated on PDA- glucose agar mediums respectively, is cultivated 3 days at 28 DEG C, photosynthetic bacteria is inoculated with
Onto beef extract-peptone agar medium, cultivated 3 days at 32 DEG C；
Step 2：The strain obtained to step 1 carries out seed culture：Bacillus subtilis that step 1 is obtained, more glutinous gemma bar
Bacterium, side spore bacillus brevis, colloid bacillus cereus are inoculated into beef extract-peptone liquid triangular flask culture medium respectively, at 28 DEG C
Lower culture 2 days, the Trichoderma harzianum that step 1 is obtained, pythium oligandrum, Trichoderma viride are inoculated into PDA- liquid of glucose triangles respectively
In bottle culture medium, cultivated 3 days at 28 DEG C, the photosynthetic bacteria that step 1 is obtained is inoculated into the training of photosynthetic bacteria dedicated liquid triangular flask
Support in base, cultivated 3 days at 32 DEG C；
Step 3：Prepare seed liquor：The strain that step 2 obtains is weighed according to following percetage by weight：It is bacillus subtilis 24%, more
Glutinous bacillus 4%, side spore bacillus brevis 14%, colloid bacillus cereus 26%, Trichoderma harzianum 6%, Trichoderma viride 6%, photosynthetic bacteria
16%th, pythium oligandrum 4%, seed liquor is obtained after well mixed；
Step 4：The seed liquor that step 3 obtains is mixed：Comprise the following steps：
A:Produce fermentation medium：By fermentation tank, drying and sterilizing handles 30min at 80 DEG C, then according to following weight percent
Number inserts raw material into fermentation tank：Molasses 1.2%, glucose 0.7%, cornstarch 1.5%, bean cake powder 1.4%, magnesium sulfate 0.02%,
Pyroligneous acid 1.4%, urea 0.11%, potassium dihydrogen phosphate 0.03%, peptone 0.09%, the water of dusty yeast 0.04% and surplus, then to hair
The microelement nutritious liquid for accounting for fermentation medium cumulative volume 0.05% is instilled in fermentation tank, fermentation medium is entered under 110 DEG C of environment
Row sterilization treatment 30min, fermentation medium is finally cooled to 25 DEG C, it is standby；
B：It is inoculated with seed liquor：The seed liquor that inoculation step 3 obtains in the fermentation medium obtained by step A, 25 DEG C of keeping temperature,
Rotating speed 160r/min, while according to seed liquor：The volume ratio of air is 1:0.8 ratio is passed through pure air, after inoculation
Fermentation medium carries out aerobic culture 24 hours, and it is 1800LX to control illumination, obtains liquid composite bacteria agent；
Step 5：Prepare solid powder：Raw material is weighed according to following percetage by weight：In the liquid composite bacteria agent obtained to step 4
Turf powder is added, according to liquid composite bacteria agent：Turf powder is 1：0.8 ratio is stirred and evenly mixed, and mixture then is put into drying
Room, which is dried to moisture, is less than 8%, then is crushed to particle diameter and reaches below 1mm, obtains solid, powdery composite bacteria agent.
Embodiment 2：A kind of composite bacteria agent for preventing and treating tobacco bacterial wilt, by seed liquor and the fermentation medium system with tank fermentation
Into the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 26%, Bacillus polymyxa 6%, side spore are short
Bacillus 16%, colloid bacillus cereus 24%, Trichoderma harzianum 4%, Trichoderma viride 4%, photosynthetic bacteria 14% and pythium oligandrum 6%, it is described
Fermentation medium consists of the following components in percentage by weight：Molasses 1.4%, glucose 0.9%, cornstarch 1.7%, bean cake powder
1.6%th, magnesium sulfate 0.04%, pyroligneous acid 1.6%, urea 0.13%, potassium dihydrogen phosphate 0.05%, peptone 0.11%, dusty yeast 0.06%
With the water of surplus；
A kind of preparation method of the composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, comprises the following steps：
Step 1：Inclined-plane culture is carried out to original strain：By bacillus subtilis, Bacillus polymyxa, side spore bacillus brevis,
Colloid bacillus cereus is inoculated on beef extract-peptone agar medium respectively, is cultivated 3 days at 32 DEG C, by Trichoderma harzianum, widow
Male rotten mould, Trichoderma viride is inoculated on PDA- glucose agar mediums respectively, is cultivated 4 days at 30 DEG C, photosynthetic bacteria is inoculated with
Onto beef extract-peptone agar medium, cultivated 5 days at 35 DEG C；
Step 2：The strain obtained to step 1 carries out seed culture：Bacillus subtilis that step 1 is obtained, more glutinous gemma bar
Bacterium, side spore bacillus brevis, colloid bacillus cereus are inoculated into beef extract-peptone liquid triangular flask culture medium respectively, at 32 DEG C
Lower culture 4 days, the Trichoderma harzianum that step 1 is obtained, pythium oligandrum, Trichoderma viride are inoculated into PDA- liquid of glucose triangles respectively
In bottle culture medium, cultivated 4 days at 30 DEG C, the photosynthetic bacteria that step 1 is obtained is inoculated into the training of photosynthetic bacteria dedicated liquid triangular flask
Support in base, cultivated 7 days at 35 DEG C；
Step 3：Prepare seed liquor：The strain that step 2 obtains is weighed according to following percetage by weight：It is bacillus subtilis 26%, more
Glutinous bacillus 6%, side spore bacillus brevis 16%, colloid bacillus cereus 24%, Trichoderma harzianum 4%, Trichoderma viride 4%, photosynthetic bacteria
14%th, pythium oligandrum 6%, seed liquor is obtained after well mixed；
Step 4：The seed liquor that step 3 obtains is mixed：Comprise the following steps：
A:Produce fermentation medium：By fermentation tank, drying and sterilizing handles 50min at 100 DEG C, then according to following weight percent
Number inserts raw material into fermentation tank：Molasses 1.4%, glucose 0.9%, cornstarch 1.7%, bean cake powder 1.6%, magnesium sulfate 0.04%,
Pyroligneous acid 1.6%, urea 0.13%, potassium dihydrogen phosphate 0.05%, peptone 0.11%, the water of dusty yeast 0.06% and surplus, then to hair
The microelement nutritious liquid for accounting for fermentation medium cumulative volume 0.07% is instilled in fermentation tank, fermentation medium is entered under 130 DEG C of environment
Row sterilization treatment 90min, fermentation medium is finally cooled to 35 DEG C, it is standby；
B：It is inoculated with seed liquor：The seed liquor that inoculation step 3 obtains in the fermentation medium obtained by step A, 35 DEG C of keeping temperature,
Rotating speed 200r/min, while according to seed liquor：The volume ratio of air is 1:0.8 ratio is passed through pure air, after inoculation
Fermentation medium carries out aerobic culture 36 hours, and it is 2200LX to control illumination, obtains liquid composite bacteria agent；
Step 5：Prepare solid powder：Raw material is weighed according to following percetage by weight：In the liquid composite bacteria agent obtained to step 4
Solid matrix is added, solid matrix is rice bran, kaolin, the mixture for the organic fertilizer that sterilizes, according to liquid composite bacteria agent：Solid-based
Matter is 1：0.8 ratio stirs and evenly mixs, and mixture then is put into hothouse is dried to moisture less than 8%, then crushes
Below 1mm is reached to particle diameter, obtains solid, powdery composite bacteria agent.
Embodiment 3：A kind of composite bacteria agent for preventing and treating tobacco bacterial wilt, by seed liquor and the fermentation medium system with tank fermentation
Into the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 25%, Bacillus polymyxa 5%, side spore are short
Bacillus 15%, colloid bacillus cereus 25%, Trichoderma harzianum 5%, Trichoderma viride 5%, photosynthetic bacteria 15% and pythium oligandrum 5%, it is described
Fermentation medium consists of the following components in percentage by weight：Molasses 1.2%, glucose 0.8%, cornstarch 1.6%, bean cake powder
1.5%th, magnesium sulfate 0.03%, pyroligneous acid 1.5%, urea 0.12%, potassium dihydrogen phosphate 0.04%, peptone 0.1%, dusty yeast 0.05%
With the water of surplus；
A kind of preparation method of the composite bacteria agent of above-mentioned preventing and treating tobacco bacterial wilt, comprises the following steps：
Step 1：Inclined-plane culture is carried out to original strain：By bacillus subtilis, Bacillus polymyxa, side spore bacillus brevis,
Colloid bacillus cereus is inoculated on beef extract-peptone agar medium respectively, is cultivated 3 days at 30 DEG C, by Trichoderma harzianum, widow
Male rotten mould, Trichoderma viride is inoculated on PDA- glucose agar mediums respectively, is cultivated 3 days at 29 DEG C, photosynthetic bacteria is inoculated with
Onto beef extract-peptone agar medium, cultivated 4 days at 33 DEG C；
Step 2：The strain obtained to step 1 carries out seed culture：Bacillus subtilis that step 1 is obtained, more glutinous gemma bar
Bacterium, side spore bacillus brevis, colloid bacillus cereus are inoculated into beef extract-peptone liquid triangular flask culture medium respectively, at 30 DEG C
Lower culture 3 days, the Trichoderma harzianum that step 1 is obtained, pythium oligandrum, Trichoderma viride are inoculated into PDA- liquid of glucose triangles respectively
In bottle culture medium, cultivated 3 days at 29 DEG C, the photosynthetic bacteria that step 1 is obtained is inoculated into the training of photosynthetic bacteria dedicated liquid triangular flask
Support in base, cultivated 4 days at 33 DEG C；
Step 3：Prepare seed liquor：The strain that step 2 obtains is weighed according to following percetage by weight：It is bacillus subtilis 25%, more
Glutinous bacillus 5%, side spore bacillus brevis 15%, colloid bacillus cereus 25%, Trichoderma harzianum 5%, Trichoderma viride 5%, photosynthetic bacteria
15% and pythium oligandrum 5%, obtain seed liquor after well mixed；
Step 4：The seed liquor that step 3 obtains is mixed：Comprise the following steps：
A:Produce fermentation medium：By fermentation tank, drying and sterilizing handles 30-50min at 80-100 DEG C, then according to following heavy
Amount percentage inserts raw material into fermentation tank：Molasses 1.2%, glucose 0.8%, cornstarch 1.6%, bean cake powder 1.5%, magnesium sulfate
0.03%th, the water of pyroligneous acid 1.5%, urea 0.12%, potassium dihydrogen phosphate 0.04%, peptone 0.1%, dusty yeast 0.05% and surplus,
The microelement nutritious liquid for accounting for fermentation medium cumulative volume 0.06% is instilled into fermentation tank again, fermentation is trained under 121 DEG C of environment
Support base and carry out sterilization treatment 30-90min, fermentation medium is finally cooled to 32 DEG C, it is standby；
B：It is inoculated with seed liquor：The seed liquor that inoculation step 3 obtains in the fermentation medium obtained by step A, 32 DEG C of keeping temperature,
Rotating speed 180r/min, while according to seed liquor：The volume ratio of air is 1:0.8 ratio is passed through pure air, after inoculation
Fermentation medium carries out aerobic culture 30 hours, and it is 2000LX to control illumination, obtains liquid composite bacteria agent；
Step 5：Prepare solid powder：Raw material is weighed according to following percetage by weight：In the liquid composite bacteria agent obtained to step 4
Solid matrix is added, solid matrix is, according to liquid composite bacteria agent：The mixture of humic acid and white carbon is 1：0.8 ratio
Stir and evenly mix, then by mixture be put into hothouse be dried to moisture be less than 8%, then be crushed to particle diameter up to 1mm with
Under, obtain solid, powdery composite bacteria agent.
On experiment of the medicament of the present invention to the prevention effect of tobacco bacterial wilt
1. subjects：Select tobacco of the kind for " Zhongyan-100 ".
2. test site：Selection is tested at Wenshan Prefecture of Yunnan Province Malipo cigarette station, and tobacco is planted on the ground year after year, soil
Earth is red soil, medium fertility.
3. reagent agent：
The reagent agent experimental design of table 1
| Medicament code | Test medicine | Extension rate |
| A | The wettable powder of the preventing and treating tobacco bacterial wilt composite bacteria agent of the 10000000000 CUF/g present invention | 500 times |
| B | 72% agricultural streptomycin soluble powder | 500 times |
| C | 20% Yekuzuo wettable powder | 400 times |
| CK | Clear water | - |
4. the process of experiment：
Started May 6 to transplant tobacco seedlings, randomly choose 30 cells, 100 plants of tobacco of plant, first time May 20 apply per cell
Medicine；Group's phase second of dispenser on June 8；Third time dispenser on June 28；Application method uses pouring root method, every plant of 200ml.
5. investigation content and method：
5.1 efficacy survey：
The investigation of cigarette strain incidence is using cell generaI investigation mode, in units of strain, in onset peak period（July 25）Carry out first
Secondary investigation, tobacco leaf picking later stage（August 19 days）Carry out second to investigate, using 5 sampling methods, 30 plants are investigated per cell, is pressed
CB/232222008《Tobacco pest and disease damage is classified and investigation method》Investigate incidence, and calculate each processing disease index with
Preventive effect effect.Test data carries out poor analyzing and processing using DPS softwares.
Tobacco bacterial wilt severity Scaling standard（In units of strain）：0 grade-complete stool is disease-free；1 grade-neck occasionally has chlorisis spot, or
Sick side 1/2 is wilting with lower blade；3 grades-neck has black streak, but no more than 1/2, or sick side 1/2-2/3 blades are wilting；5 grades-
Neck black streak is not reached at the top of stem more than 1/2, or sick side 2/3 is wilting with blade；7 grades-neck black streak reaches
At the top of stem, or diseased plant blade is all wilting；9 grades-diseased plant is substantially withered.
Preventive effect is calculated by following equation：
5.2 physiological characters are investigated：
(July 18) in units of strain, using 5 point samplings, 10 plants is investigated per cell, measures plant height, the stem of cigarette strain after topping
Enclose, the length and width value of pitch, effective blade number and blade.
6. test results and analysis：
6.1 pharmacodynamic results and analysis：
To the prevention effect of tobacco bacterial wilt under the processing of the different agents of table 2
Test of pesticide effectiveness result as shown above, in the 1st investigation (July 25), preventing and treating tobacco bacterial wilt effect most preferably 100
The wettable powder of the preventing and treating tobacco bacterial wilt composite bacteria agent of the hundred million CUF/g present invention, prevention effect reach 69.21%, are secondly
72% agricultural streptomycin sulphate soluble powder and 20% Yekuzuo pulvis, preventive effect are respectively 45.14%, 44.21%.2nd investigation
（August 19 days) in, 72% agricultural streptomycin sulphate soluble powder and 20% Yekuzuo pulvis preventive effect respectively reach 67.94%, 66.
41%, both differences are not notable, and the wettable powder of the preventing and treating tobacco bacterial wilt composite bacteria agent of the 10000000000 CUF/g present invention is anti-
Control effect and rise to 8O.12%, be significantly higher than the preventive effect of 72% agricultural streptomycin sulphate soluble powder and 20% Yekuzuo pulvis.
6.2 tobacco economical character results and analysis：
The lower tobacco economical character of the different agents of table 3 processing
As shown above, it was found from economical character investigation, the 10000000000 CUF/g present invention's is anti-for the result of the test of tobacco economical character
The economical character of the wettable powder treatment region tobacco of tobacco bacterial wilt composite bacteria agent is controlled, with other processing and blank control phase
Than all there is obvious advantage in the length and width etc. of plant height, stem girth, leaf；Show that the preventing and treating tobacco through the 10000000000 CUF/g present invention is blue or green
After the wettable powder processing of rot composite bacteria agent, the improvement of tobacco economical character can be remarkably promoted.
7. conclusion (of pressure testing)：
3 kinds of medicaments have control action by root-pouring method to tobacco bacterial wilt.The preventing and treating tobacco of the wherein 10,000,000,000 CUF/g present invention
The wettable powder of bacterial wilt composite bacteria agent is best to the prevention effect of tobacco bacterial wilt, and the 2nd time investigation preventive effect is up to 80.02%,
And there is stimulating growth to tobacco seedlings, significantly improve the economical character of tobacco seedlings, substantially increase yield of tobacco.By
In tobacco bacterial wilt primary infection source based on crop rhizosphere soils, and pathogen is directly entered host's vascular bundle and caused harm, therefore medicine
Agent preventing and treating must be carried out in time after tobacco transplant, and pouring root capacity is 100-300ml/ strains, continuous more than 3 times, Fang Keda
To the target for effectively controlling it to cause harm.Meanwhile 10,000,000,000 CUF/g the present invention preventing and treating tobacco bacterial wilt composite bacteria agent wettable
Pulvis is biological agent, and it is environmentally safe after use, it is ensured that the green production of tobacco.
Claims (4)
- A kind of 1. composite bacteria agent for preventing and treating tobacco bacterial wilt, it is characterised in that：By seed liquor and the fermentation medium with tank fermentation It is made, the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 22-28%, Bacillus polymyxa 2- 8%th, side spore bacillus brevis 12-18%, colloid bacillus cereus 22-28%, Trichoderma harzianum 2-8%, Trichoderma viride 2-8%, photosynthetic bacteria 12-18% and pythium oligandrum 2-8%；The fermentation medium consists of the following components in percentage by weight：Molasses 1.2-1.4%, Portugal Grape sugar 0.7-0.9%, cornstarch 1.5-1.7%, bean cake powder 1.4-1.6%, magnesium sulfate 0.02-0.04%, pyroligneous acid 1.4-1.6%, Urea 0.11-0.13%, potassium dihydrogen phosphate 0.03-0.05%, peptone 0.09-0.11%, dusty yeast 0.04-0.06% and surplus Water.
- 2. the composite bacteria agent of preventing and treating tobacco bacterial wilt according to claim 1, it is characterised in that：Sent out by seed liquor and with tank The fermentation medium of ferment is made, and the seed liquor is made up of the strain of following percentage by weight：Bacillus subtilis 25%, stick more Bacillus 5%, side spore bacillus brevis 15%, colloid bacillus cereus 25%, Trichoderma harzianum 5%, Trichoderma viride 5%, photosynthetic bacteria 15% With pythium oligandrum 5%；The fermentation medium comprises the following raw materials by weight percent：Molasses 1.2%, glucose 0.8%, jade Rice starch 1.6%, bean cake powder 1.5%, magnesium sulfate 0.03%, pyroligneous acid 1.5%, urea 0.12%, potassium dihydrogen phosphate 0.04%, peptone 0.1%th, dusty yeast 0.05% and the water of surplus.
- A kind of 3. method for the composite bacteria agent for preparing preventing and treating tobacco bacterial wilt according to claim 1, it is characterised in that：Bag Include following steps：Step 1：Inclined-plane culture is carried out to original strain：By bacillus subtilis, Bacillus polymyxa, side spore bacillus brevis, Colloid bacillus cereus is inoculated on beef extract-peptone agar medium respectively, is cultivated 2-3 days at 28-32 DEG C, will breathe out thatch wood Mould, pythium oligandrum, Trichoderma viride are inoculated on PDA- glucose agar mediums respectively, are cultivated 3-4 days at 28-30 DEG C, will Photosynthetic bacteria is inoculated on beef extract-peptone agar medium, is cultivated 3-5 days at 32-35 DEG C；Step 2：The strain obtained to step 1 carries out seed culture：Bacillus subtilis that step 1 is obtained, more glutinous gemma bar Bacterium, side spore bacillus brevis, colloid bacillus cereus are inoculated into beef extract-peptone liquid triangular flask culture medium respectively, in 28- Cultivated 2-4 days at 32 DEG C, the Trichoderma harzianum that step 1 is obtained, pythium oligandrum, Trichoderma viride are inoculated into PDA- Glucose Liquids respectively In body triangular flask culture medium, cultivated 3-4 days at 28-30 DEG C, the photosynthetic bacteria that step 1 is obtained is inoculated into the special liquid of photosynthetic bacteria In body triangular flask culture medium, cultivated 3-7 days at 32-35 DEG C；Step 3：Prepare seed liquor：The strain that step 2 obtains is weighed according to following percetage by weight：Bacillus subtilis 22- 28%th, Bacillus polymyxa 2-8%, side spore bacillus brevis 12-18%, colloid bacillus cereus 22-28%, Trichoderma harzianum 2-8%, green Color trichoderma 2-8%, photosynthetic bacteria 12-18%, pythium oligandrum 2-8%, seed liquor is obtained after well mixed；Step 4：The seed liquor that step 3 obtains is mixed：Comprise the following steps：A:Produce fermentation medium：By fermentation tank, drying and sterilizing handles 30-50min at 80-100 DEG C, then according to following heavy Amount percentage inserts raw material into fermentation tank：Molasses 1.2-1.4%, glucose 0.7-0.9%, cornstarch 1.5-1.7%, dregs of beans Powder 1.4-1.6%, magnesium sulfate 0.02-0.04%, pyroligneous acid 1.4-1.6%, urea 0.11-0.13%, potassium dihydrogen phosphate 0.03- 0.05%th, peptone 0.09-0.11%, dusty yeast 0.04-0.06% and surplus water, then instilled into fermentation tank and account for fermented and cultured Base cumulative volume 0.05-0.07% microelement nutritious liquid, sterilization treatment is carried out to fermentation medium under 110-130 DEG C of environment 30-90min, fermentation medium is finally cooled to 25-35 DEG C, it is standby；B：It is inoculated with seed liquor：The seed liquor that inoculation step 3 obtains in the fermentation medium obtained by step A, keeping temperature 25-35 DEG C, rotating speed 160-200r/min, while according to seed liquor：The volume ratio of air is 1:0.8 ratio is passed through pure air, docking Fermentation medium after kind carries out aerobic culture 24-36 hours, and it is 1800-2200LX to control illumination, obtains liquid composite bacteria agent；Step 5：Prepare solid powder：Raw material is weighed according to following percetage by weight：In the liquid composite bacteria agent obtained to step 4 Solid matrix is added, according to liquid composite bacteria agent：Solid matrix is 1：0.8 ratio stirs and evenly mixs, and is then put into mixture Hothouse, which is dried to moisture, is less than 8%, then is crushed to particle diameter and reaches below 1mm, obtains solid, powdery composite bacteria agent.
- 4. the method for the composite bacteria agent according to claim 3 for preparing preventing and treating tobacco bacterial wilt, it is characterised in that：It is described solid Body matrix is the one or more in turf powder, rice bran, kaolin, sterilizing organic fertilizer, humic acid, white carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943241.3A CN107723258B (en) | 2017-10-11 | 2017-10-11 | Composite microbial inoculum for preventing and treating tobacco bacterial wilt and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710943241.3A CN107723258B (en) | 2017-10-11 | 2017-10-11 | Composite microbial inoculum for preventing and treating tobacco bacterial wilt and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107723258A true CN107723258A (en) | 2018-02-23 |
| CN107723258B CN107723258B (en) | 2021-03-12 |
Family
ID=61210900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710943241.3A Active CN107723258B (en) | 2017-10-11 | 2017-10-11 | Composite microbial inoculum for preventing and treating tobacco bacterial wilt and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107723258B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110250209A (en) * | 2019-06-19 | 2019-09-20 | 云南省微生物发酵工程研究中心有限公司 | A kind of microbial bacterial agent and its preparation method and application with the mildew and rot sick function of prevention and treatment tobacco |
| CN110384247A (en) * | 2019-08-07 | 2019-10-29 | 云南省烟草公司曲靖市公司 | Tobacco leaf goes rotten sick biological control method in a kind of Tobacco Leaf Curing |
| CN110683875A (en) * | 2019-11-13 | 2020-01-14 | 贵州中烟工业有限责任公司 | Biological bacterial fertilizer for preventing bacterial wilt of flue-cured tobacco, preparation method and application |
| CN112522149A (en) * | 2020-12-11 | 2021-03-19 | 湖南稼陶农业科技有限公司 | Compound microbial agent capable of preventing and treating tobacco diseases |
| CN113355260A (en) * | 2021-04-23 | 2021-09-07 | 广西壮族自治区农业科学院 | Compound microbial inoculum for preventing and treating banana wilt and preparation method thereof |
| CN113699048A (en) * | 2021-08-25 | 2021-11-26 | 云南省烟草公司昆明市公司 | Microbial compound bacterium agent for preventing and treating black shank and bacterial wilt of solanaceae plants and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575574A (en) * | 2009-06-11 | 2009-11-11 | 刘和现 | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection |
| WO2012147928A1 (en) * | 2011-04-28 | 2012-11-01 | 国立大学法人広島大学 | Agent for preventing bacterial wilt disease, and method for preventing bacterial wilt disease |
| KR20130101312A (en) * | 2012-03-05 | 2013-09-13 | 충북대학교 산학협력단 | Methylobacterium spp. cbmb15 or cbmb27 strain having controlling activity against bacterial wilt disease of tomato and uses thereof |
| CN105036986A (en) * | 2015-08-17 | 2015-11-11 | 苏州玖沃生物科技有限公司 | Microbial fertilizer resistant to diseases and insects and preparation method thereof |
| CN105123784A (en) * | 2015-09-18 | 2015-12-09 | 昆明线敌生物科技有限公司 | Traditional Chinese medicine preparation for preventing and treating ginger bacterial wilt and preparation method of traditional Chinese medicine preparation |
| CN105875652A (en) * | 2015-11-03 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparing method of tobacco floral leaf geramine for preventing and controlling tobacco mosaic virus |
| CN106465734A (en) * | 2016-08-29 | 2017-03-01 | 佛山市艳晖生物科技有限公司 | A kind of preventing and treating tobacco bacterial wilt fermentation liquid and preparation method thereof |
| CN106471982A (en) * | 2016-09-28 | 2017-03-08 | 河南省农业科学院植物营养与资源环境研究所 | Take into account soil quality and improve the tobacco cultivation fertilizing method improving with quality of tobacco |
| CN107083348A (en) * | 2017-06-23 | 2017-08-22 | 中国热带农业科学院环境与植物保护研究所 | A kind of composite bacteria agent for having soil improvement and bacterial wilt preventive and therapeutic effect concurrently and preparation method thereof |
-
2017
- 2017-10-11 CN CN201710943241.3A patent/CN107723258B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575574A (en) * | 2009-06-11 | 2009-11-11 | 刘和现 | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection |
| WO2012147928A1 (en) * | 2011-04-28 | 2012-11-01 | 国立大学法人広島大学 | Agent for preventing bacterial wilt disease, and method for preventing bacterial wilt disease |
| KR20130101312A (en) * | 2012-03-05 | 2013-09-13 | 충북대학교 산학협력단 | Methylobacterium spp. cbmb15 or cbmb27 strain having controlling activity against bacterial wilt disease of tomato and uses thereof |
| CN105036986A (en) * | 2015-08-17 | 2015-11-11 | 苏州玖沃生物科技有限公司 | Microbial fertilizer resistant to diseases and insects and preparation method thereof |
| CN105123784A (en) * | 2015-09-18 | 2015-12-09 | 昆明线敌生物科技有限公司 | Traditional Chinese medicine preparation for preventing and treating ginger bacterial wilt and preparation method of traditional Chinese medicine preparation |
| CN105875652A (en) * | 2015-11-03 | 2016-08-24 | 鹤壁市禾盛生物科技有限公司 | Preparing method of tobacco floral leaf geramine for preventing and controlling tobacco mosaic virus |
| CN106465734A (en) * | 2016-08-29 | 2017-03-01 | 佛山市艳晖生物科技有限公司 | A kind of preventing and treating tobacco bacterial wilt fermentation liquid and preparation method thereof |
| CN106471982A (en) * | 2016-09-28 | 2017-03-08 | 河南省农业科学院植物营养与资源环境研究所 | Take into account soil quality and improve the tobacco cultivation fertilizing method improving with quality of tobacco |
| CN107083348A (en) * | 2017-06-23 | 2017-08-22 | 中国热带农业科学院环境与植物保护研究所 | A kind of composite bacteria agent for having soil improvement and bacterial wilt preventive and therapeutic effect concurrently and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| MONCHAN MAKETON ET AL.: ""GREENHOUSE EVALUATION OF BACILLUS SUBTILIS AP-01 AND TRICHODERMA HARZIANUM AP-001 IN CONTROLLING TOBACCO DISEASES"", 《BRAZILIAN JOURNAL OF MICROBIOLOGY》 * |
| 陈利锋等: "《农业植物病理学》", 28 February 2007, 中国农业出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110250209A (en) * | 2019-06-19 | 2019-09-20 | 云南省微生物发酵工程研究中心有限公司 | A kind of microbial bacterial agent and its preparation method and application with the mildew and rot sick function of prevention and treatment tobacco |
| CN110250209B (en) * | 2019-06-19 | 2021-05-28 | 云南省微生物发酵工程研究中心有限公司 | Microbial agent with function of preventing and treating mildew and rot of tobacco as well as preparation method and application of microbial agent |
| CN110384247A (en) * | 2019-08-07 | 2019-10-29 | 云南省烟草公司曲靖市公司 | Tobacco leaf goes rotten sick biological control method in a kind of Tobacco Leaf Curing |
| CN110384247B (en) * | 2019-08-07 | 2020-12-22 | 云南省烟草公司曲靖市公司 | Biological control method for tobacco rot disease in tobacco leaf baking process |
| CN110683875A (en) * | 2019-11-13 | 2020-01-14 | 贵州中烟工业有限责任公司 | Biological bacterial fertilizer for preventing bacterial wilt of flue-cured tobacco, preparation method and application |
| CN112522149A (en) * | 2020-12-11 | 2021-03-19 | 湖南稼陶农业科技有限公司 | Compound microbial agent capable of preventing and treating tobacco diseases |
| CN113355260A (en) * | 2021-04-23 | 2021-09-07 | 广西壮族自治区农业科学院 | Compound microbial inoculum for preventing and treating banana wilt and preparation method thereof |
| CN113699048A (en) * | 2021-08-25 | 2021-11-26 | 云南省烟草公司昆明市公司 | Microbial compound bacterium agent for preventing and treating black shank and bacterial wilt of solanaceae plants and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107723258B (en) | 2021-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107723258A (en) | Prevent and treat composite bacteria agent of tobacco bacterial wilt and preparation method thereof | |
| CN103819267B (en) | For the microbial-bacterial fertilizer and preparation method thereof of peanut cultivation | |
| CN105462878B (en) | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN109896900A (en) | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer | |
| CN103145461A (en) | Composite microbial bacterial fertilizer with functions of disease prevention, growth promotion and yield increase and application thereof | |
| CN108277177B (en) | Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application | |
| CN108821875A (en) | A kind of preparation method of high-activity microorganism fertilizer | |
| CN109824433A (en) | It is a kind of prevent and treat Strawberry anthracnose microbial organic fertilizer and application | |
| CN101463339B (en) | Inocula for preventing apple fungous diseases and increasing yield, and special strain therefore and use thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN112794738A (en) | Special bacterial fertilizer for selenium-rich tea and preparation method and application thereof | |
| CN108440037A (en) | A kind of growth promoting increases income organic fertilizer and preparation method thereof | |
| CN107382511A (en) | A kind of Chinese herbal medicine fermentation fertilizer for preventing and treating cucumber fusarium axysporum and preparation method thereof | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN112159770B (en) | Method for preparing biocontrol bacterial fertilizer by fermenting decayable waste through Bacillus belgii | |
| CN108383653A (en) | A kind of method and bio-organic fertilizer preparing fertilizer using stalk | |
| CN110791459B (en) | Bacillus subtilis for preventing and controlling continuous cropping lily soil-borne blight and application thereof | |
| CN104496575A (en) | Organic fertilizer for resisting continuous cropping obstacles of chuzhou chrysanthemum and preparation method of organic fertilizer | |
| CN107857681A (en) | A kind of special fertilizer for cole and preparation method and application | |
| CN111892440A (en) | Microbial fertilizer for preventing and treating potato scab and preparation method and use method thereof | |
| CN111072420A (en) | Compound amino acid microbial agent and preparation method thereof | |
| CN114149808B (en) | Soil conditioner for preventing and treating tobacco bacterial wilt and improving proportion of middle and upper tobacco leaves and preparation method thereof | |
| CN110218122A (en) | Dedicated ecological organic fertilier of one planting fruit-trees and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |