CN112522149A - Compound microbial agent capable of preventing and treating tobacco diseases - Google Patents
Compound microbial agent capable of preventing and treating tobacco diseases Download PDFInfo
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- CN112522149A CN112522149A CN202011436871.XA CN202011436871A CN112522149A CN 112522149 A CN112522149 A CN 112522149A CN 202011436871 A CN202011436871 A CN 202011436871A CN 112522149 A CN112522149 A CN 112522149A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
Abstract
The invention discloses a compound microbial agent capable of preventing and treating tobacco diseases, which is prepared by mixing and fermenting 5-10 parts of compound microbial powder, 10-20 parts of special nutrient solution and 80-90 parts of tap water by weight. The compound microbial powder is prepared by mixing bacillus subtilis powder, yeast powder, photosynthetic bacteria powder, bacillus licheniformis powder, bacillus laterosporus powder, jelly-like bacillus powder and basilar bacteria powder in equal parts. The special nutrient solution is prepared by mixing bamboo liquid and grass powder. The compound microbial agent has obvious control effects on the black stem disease, bacterial wilt and TMV of tobacco.
Description
Technical Field
The invention relates to a compound microbial agent capable of preventing and treating tobacco diseases.
Background
In the tobacco planting process, an important factor influencing the growth of tobacco is tobacco diseases, wherein the southern Hunan area mainly comprises black stem diseases, bacterial wilt and TMV.
The causes of these tobacco diseases are mainly: the tobacco fields are generally continuously planted for a long time, so that corresponding germs can be propagated in successive years and accumulated in a large amount of soil to form diseased soil, and the diseased soil of the tobacco fields in the tobacco fields can cause annual disease attack of tobacco; in addition, the large amount of applied fertilizer can also stimulate the growth of fusarium, verticillium and rhizoctonia in soil-borne bacteria, and aggravate the occurrence of tobacco root diseases.
How to prevent and treat the harm of tobacco diseases becomes a major subject which is urgently needed to be solved in the current tobacco production link.
The method is an effective means for effectively preventing and treating the tobacco diseases by improving the structure of the soil flora, namely forming the dominant flora in the soil microenvironment, thereby effectively preventing and treating the growth and the propagation of pathogenic bacteria at the roots of the tobacco so as to achieve the aims of biological prevention and treatment and promotion of nutrient absorption of crops.
For this reason, the applicant has conducted long-term research and research. Through repeated field comparison tests, the compound microbial agent capable of preventing and treating tobacco diseases is developed finally.
Disclosure of Invention
The invention aims to solve the technical problem of providing a compound microbial agent capable of preventing and treating tobacco diseases.
In order to solve the technical problem, the compound microbial agent capable of preventing and treating tobacco diseases is prepared by mixing and fermenting 5-10 parts by weight of compound microbial powder, 10-20 parts by weight of special nutrient solution and 80-90 parts by weight of tap water.
The compound microbial powder is prepared by mixing bacillus subtilis powder, yeast powder, photosynthetic bacteria powder, bacillus licheniformis powder, bacillus laterosporus powder, jelly-like bacillus powder and basilar bacteria powder in equal parts.
The special nutrient solution is bamboo liquid.
The special nutrient solution is prepared from grass powder of the grass of the cystic caryopsis.
The special nutrient solution is prepared by mixing bamboo liquid and grass powder.
The special nutrient solution is prepared by mixing 20 parts by weight of bamboo liquid and 4 parts by weight of grass powder.
The bamboo liquid is obtained by high-temperature water boiling, namely cutting the phyllostachys pubescens into blocks and boiling the blocks for 1 to 2 hours at the temperature of 100 ℃ and 120 ℃.
The preparation method of the grass powder comprises the following steps: sun drying the grass leaves of the bag grass, and crushing to obtain the grass tea.
After the compound microbial powder, the special nutrient solution and tap water are mixed and fermented, the pH value is adjusted to 5.8-6.5.
Is prepared by mixing and fermenting 5 parts of composite microbial powder, 10 parts of special nutrient solution and 85 parts of tap water.
The invention is prepared by compound microorganism bacterium powder, special nutrient solution and tap water. The compound microbial powder contains multiple functional bacteria, wherein active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of bacillus subtilis have obvious inhibition effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection; the yeast is a unicellular fungus, is a tiny unicellular microorganism invisible to naked eyes, can ferment sugar into alcohol and carbon dioxide, is a typical heterotrophic facultative anaerobic microorganism, can survive under aerobic and anaerobic conditions, is a natural leavening agent, plays a role in promoting fermentation by medium level in the invention, and derivatives thereof can activate plant cells and promote root differentiation; the photosynthetic bacteria can eliminate bad peculiar smell, effectively decompose or absorb harmful substances such as ammoniacal nitrogen, nitroso nitrogen, hydrogen sulfide and the like, promote the circulation of organic matters, are important forces for fertilizing soil and promoting the growth of plants, and can promote the photosynthesis of the plants; the bacillus licheniformis has stronger protease, amylase and lipase activities, and the derivative of the bacillus licheniformis can inhibit and kill pathogenic bacteria; the bacillus laterosporus can produce bacteriostatic protein, bacteriostatic protease and the like, and can inhibit the growth of pathogenic fungi; the bacillus mucilaginosus can quickly form beneficial flora in rhizosphere soil, effectively inhibit the propagation of harmful and pathogenic microorganisms in the soil, and decompose and release soluble phosphorus and potassium elements and medium and trace elements such as calcium, sulfur, magnesium, iron, zinc, molybdenum, manganese and the like, thereby not only improving the soil fertility, but also providing absorbable and utilizable nutrient elements for crops. The functional bacteria in the liquid compound microbial agent are mutually promoted and supplemented, beneficial bacteria groups are mutually cooperated and act together, and the effect of resisting soil-borne diseases is far greater than that of a single strain.
The composite microbial agent contains special nutrient solution of nutrient materials required by the composite microbial bacteria, when the liquid composite microbial agent is applied to a field, the composite biological functional bacteria are propagated explosively under the conditions of proper temperature and humidity in soil, and the dominant flora is rapidly formed in the microenvironment of the rhizosphere soil of crops, so that the growth and propagation of pathogenic bacteria at the root of tobacco are effectively inhibited, the biological control effect is achieved, and the nutrient absorption of the crops is promoted.
The inventor finds that the bamboo liquid and the grass powder can be used as nutrient materials required by the compound microorganism bacteria and can promote the propagation of various functional bacteria of the compound organism.
The bamboo liquid is a nutrient prebiotic for microbial growth, is a probiotic food, is beneficial to the growth and reproduction of beneficial bacteria, can be used as a dietary supplement, helps to restore gastrointestinal tract function, and promotes the growth of beneficial bacteria. Through tests, the inventor finds that the bamboo liquid can be used as nutrient materials required by the compound microorganism bacteria, can promote the propagation of various functional bacteria of the compound organisms, and can be used for preparing the compound microorganism bacteria agent to prevent and control the black stem disease, the bacterial wilt and the TMV of the tobacco.
The glume grass is a common wild grass in southern Hunan, grows in wet lands, fields, water sides and the like beside roads and is harmful to the growth of crops; in the crop planting process, the crops can be eradicated. The inventor finds that the grass of the bag glume is dried in the sun and piled to form fertilizer during weeding, and the insect pests of the tobacco are less. Therefore, the caryopsis nodorum is used for the test, and is used as a component of special nutrient solution of nutrient materials required by the compound microorganism bacteria to be mixed with the bamboo liquid for use, so that an unexpected effect is obtained, and the caryopsis nodorum has obvious prevention and treatment effects on the black stem disease, the bacterial wilt and the TMV of the tobacco.
The special nutrient solution is preferably prepared by mixing bamboo liquid and grass powder, wherein the grass powder is an important component. In the test process, the bamboo liquid and the bag glume grass powder are used independently, and the bamboo liquid and the bag glume grass powder are mixed for use, and through comparison, the effect of mixing the bamboo liquid and the bag glume grass powder is better than that of using the bamboo liquid and the bag glume grass powder independently.
Through inspection, the effective viable count (cfu) of the liquid compound microbial agent is more than or equal to 0.6 hundred million/ml. Is harmless to human and livestock.
The recommended use method of the compound microbial agent comprises the following steps: the composite microbial inoculum of the invention is mixed with water according to the weight ratio of 1: 1000, and the root of the tobacco plant is irrigated respectively according to the dosage of 500 ml/mu in the root-applying period and the dosage of 500 ml/mu in the vigorous growing period of the tobacco plant.
The inventor makes several comparative experiments on the compound microbial agent, and proves that the compound microbial agent has obvious effect of preventing and treating tobacco diseases (black stem disease, bacterial wilt and TMV) by promoting the propagation of effective microorganisms in soil.
First, a comparative test of the ability of the compound microbial agent of the present invention to control tobacco diseases is performed.
Test group T1, the composite microbial inoculum (containing bamboo liquid alone) of the invention is mixed with water according to the weight ratio of 1: 1000, and the mixture is respectively irrigated to the roots of tobacco plants according to the dosage of 500 ml/mu in the rooting period and the dosage of 500 ml/mu in the vigorous growing period of the tobacco plants.
Test group T2, the composite microbial inoculum (containing the grass powder of the bursa glume separately) is mixed with water according to the weight ratio of 1: 1000, and the mixture is respectively irrigated to the roots of tobacco plants according to the dosage of 500 ml/mu in the root-applying period of the tobacco plants and the dosage of 500 ml/mu in the vigorous growth period.
Test group T3, the composite microbial inoculum (bamboo liquid and grass powder) of the invention is mixed with water according to the weight ratio of 1: 1000, and the mixture is irrigated to the root of the tobacco plant according to the dosage of 500 ml/mu in the rooting period and the dosage of 500 ml/mu in the vigorous growing period of the tobacco plant respectively.
Compared with a group CK, the biological control agent is used for 1 kg per mu in a conventional control method, and the biological control agent is mixed with base fertilizers for tobacco and subjected to hole application before special tobacco seedlings are transplanted.
Test site: guiyang county. Yunyan 87 was the reference variety. Investigation time: year 2019, month 6.
TABLE 1 disease control Condition
The disease rate calculation formula is as follows: incidence (%) × 100 (number of diseased plants/number of investigated plants)
As can be seen from Table 1, the compound microbial agent has obvious capacity of preventing and treating black stem diseases, bacterial wilt and TMV of tobacco diseases; in the compound microbial agent, the effect of mixing the bamboo liquid and the grass powder is better than that of singly using the bamboo liquid and the grass powder.
And a second test is a comparison of the use time of the compound microbial agent for preventing and treating TMV diseases of tobacco and a test of the restoration condition of diseased plants.
One test group is that the composite microbial inoculum (bamboo liquid and grass powder are mixed) is used one day before the tobacco plants are inoculated with TMV, the composite microbial inoculum is mixed with water according to the weight ratio of 1: 1000, the tobacco plants are sprayed, the front and the back of the tobacco leaves are sprayed with moisture, the roots of the tobacco plants are irrigated, the treatment is carried out once every 5 days, and the treatment is carried out three times continuously, wherein the dosage is 500 ml/mu each time.
And in the second test group, the tobacco plants are inoculated with TMV, and one day later, the compound microbial agent (bamboo liquid and grass powder are mixed) is used, the compound microbial agent is mixed with water according to the weight ratio of 1: 1000, the mixture is sprayed on the tobacco plants, the front and the back of the tobacco leaves are sprayed with moisture, the roots of the tobacco plants are irrigated, the treatment is carried out once every 5 days, and the treatment is carried out three times continuously, wherein the dosage of each time is 500 ml/mu.
Control group: conventional treatment, the complex microbial agent of the invention is not used.
Test site: guiyang county Fangyuan Zhengyuan village, test variety Yunyan 87. A test mode of three times of repeated block arrangement of double-factor random blocks in a field plot is adopted to carry out a comparative test on the prevention and control of tobacco mosaic disease.
TABLE 2 time of use comparison (2019 survey data for technical center project inspection group of 6 months and 7 days)
The data in Table 2 show that the compound microbial agent has strong inhibition effect on TMV, and the effect of drug application prevention before infection of tobacco plants with TMV is much better than that of drug application prevention after infection of tobacco plants with TMV.
TABLE 3 statistical analysis table of repair rate of TMV diseased plants
The formula for calculating the restoration rate of the diseased plant is as follows: the diseased plant repair rate (%) — diseased plant recovery number/diseased plant × 100.
As can be seen from Table 3, the complex microbial inoculant provided by the invention has an obvious repairing effect on TMV diseased strains.
Detailed Description
The compound microbial agent capable of preventing and treating tobacco diseases is prepared by mixing and fermenting 5-10 parts by weight of compound microbial powder, 10-20 parts by weight of special nutrient solution and 80-90 parts by weight of tap water.
Preferably prepared by mixing and fermenting 5 parts by weight of composite microbial powder, 10 parts by weight of special nutrient solution and 85 parts by weight of tap water. Fermentation time: fermenting at normal temperature for 1 hr.
The composite microbial powder is prepared by mixing bacillus subtilis powder, yeast powder, photosynthetic bacteria powder, bacillus licheniformis powder, bacillus laterosporus powder, jelly-like bacillus powder and basilar bacteria powder in equal parts.
The special nutrient solution can be bamboo liquid.
The special nutrient solution can also be prepared from the grass powder of the cystic glume.
The special nutrient solution is preferably prepared by mixing bamboo liquid and grass powder. Thus, the effect is the best. The special nutrient solution is preferably prepared by mixing 20 parts by weight of bamboo liquid and 4 parts by weight of grass powder.
The bamboo liquid is obtained by high-temperature water boiling, namely cutting the phyllostachys pubescens into pieces, and boiling the bamboo pieces for 1 to 2 hours at the temperature of 120 ℃ with water of 100-.
The preparation method of the grass powder comprises the following steps: sun drying the grass leaves of the bag grass, and crushing to obtain the grass tea.
After the compound microbial powder, the special nutrient solution and tap water are mixed and fermented, the pH value is adjusted to 5.8-6.5.
Claims (10)
1. A compound microbial agent capable of preventing and treating tobacco diseases is characterized in that: is prepared by mixing and fermenting 5-10 parts of composite microbial powder, 10-20 parts of special nutrient solution and 80-90 parts of tap water by weight.
2. The compound microbial agent capable of controlling tobacco diseases according to claim 1, characterized in that: the compound microbial powder is prepared by mixing bacillus subtilis powder, yeast powder, photosynthetic bacteria powder, bacillus licheniformis powder, bacillus laterosporus powder, jelly-like bacillus powder and basilar bacteria powder in equal parts.
3. The compound microbial agent capable of controlling tobacco diseases according to claim 1, characterized in that: the special nutrient solution is bamboo liquid.
4. The compound microbial agent capable of controlling tobacco diseases according to claim 1, characterized in that: the special nutrient solution is prepared from grass powder of the grass of the cystic caryopsis.
5. The complex microbial inoculant for controlling tobacco diseases according to claim 1, 3 or 4, wherein: the special nutrient solution is prepared by mixing bamboo liquid and grass powder.
6. The compound microbial agent capable of controlling tobacco diseases according to claim 5, wherein: the special nutrient solution is prepared by mixing 20 parts by weight of bamboo liquid and 4 parts by weight of grass powder.
7. The compound microbial agent capable of controlling tobacco diseases according to claim 3, wherein: the bamboo liquid is obtained by high-temperature water boiling, namely cutting the phyllostachys pubescens into blocks and boiling the blocks for 1 to 2 hours at the temperature of 100 ℃ and 120 ℃.
8. The compound microbial agent capable of controlling tobacco diseases according to claim 4, wherein: the preparation method of the grass powder comprises the following steps: sun drying the grass leaves of the bag grass, and crushing to obtain the grass tea.
9. The compound microbial agent capable of controlling tobacco diseases according to claim 1, characterized in that: after the compound microbial powder, the special nutrient solution and tap water are mixed and fermented, the pH value is adjusted to 5.8-6.5.
10. The compound microbial agent capable of controlling tobacco diseases according to claim 1, characterized in that: is prepared by mixing and fermenting 5 parts of composite microbial powder, 10 parts of special nutrient solution and 85 parts of tap water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011436871.XA CN112522149A (en) | 2020-12-11 | 2020-12-11 | Compound microbial agent capable of preventing and treating tobacco diseases |
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| CN202011436871.XA CN112522149A (en) | 2020-12-11 | 2020-12-11 | Compound microbial agent capable of preventing and treating tobacco diseases |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616212A (en) * | 2019-09-25 | 2019-12-27 | 内蒙古昆明卷烟有限责任公司 | Application of bacillus licheniformis LCCC10150 in production of beta-glucanase and tobacco fermentation |
| CN115777417A (en) * | 2022-12-27 | 2023-03-14 | 中国烟草总公司四川省公司 | Biological control method for preventing and treating tobacco climate spots |
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| CN109402012A (en) * | 2018-11-19 | 2019-03-01 | 四川省烟草公司凉山州公司 | It is a kind of to prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof |
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| CN103740613A (en) * | 2013-12-20 | 2014-04-23 | 毕景阳 | Preparation method for biological active bacterium agent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616212A (en) * | 2019-09-25 | 2019-12-27 | 内蒙古昆明卷烟有限责任公司 | Application of bacillus licheniformis LCCC10150 in production of beta-glucanase and tobacco fermentation |
| CN115777417A (en) * | 2022-12-27 | 2023-03-14 | 中国烟草总公司四川省公司 | Biological control method for preventing and treating tobacco climate spots |
| CN115777417B (en) * | 2022-12-27 | 2023-11-21 | 中国烟草总公司四川省公司 | Biological control method for controlling tobacco climate spots |
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