CN109402012A - It is a kind of to prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof - Google Patents
It is a kind of to prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN109402012A CN109402012A CN201811378223.6A CN201811378223A CN109402012A CN 109402012 A CN109402012 A CN 109402012A CN 201811378223 A CN201811378223 A CN 201811378223A CN 109402012 A CN109402012 A CN 109402012A
- Authority
- CN
- China
- Prior art keywords
- disease
- organic fertilizer
- biological organic
- composite bacteria
- microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 201000010099 disease Diseases 0.000 title claims abstract description 112
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 112
- 230000000813 microbial effect Effects 0.000 title claims abstract description 71
- 241000208125 Nicotiana Species 0.000 title claims abstract description 55
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 55
- 241000233866 Fungi Species 0.000 title claims abstract description 50
- 241000894006 Bacteria Species 0.000 title claims abstract description 47
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 47
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 46
- 239000002131 composite material Substances 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 45
- 230000004151 fermentation Effects 0.000 claims abstract description 45
- 239000002054 inoculum Substances 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 19
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 18
- 241000223261 Trichoderma viride Species 0.000 claims abstract description 18
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims description 20
- 238000010564 aerobic fermentation Methods 0.000 claims description 17
- 230000002265 prevention Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 241000228245 Aspergillus niger Species 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 239000003337 fertilizer Substances 0.000 abstract description 5
- 239000002068 microbial inoculum Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 235000019504 cigarettes Nutrition 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 244000052769 pathogen Species 0.000 description 11
- 230000001717 pathogenic effect Effects 0.000 description 11
- 239000002689 soil Substances 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 238000000034 method Methods 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 4
- 230000003698 anagen phase Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000012286 Chitinases Human genes 0.000 description 2
- 108010022172 Chitinases Proteins 0.000 description 2
- 241001002356 Valeriana edulis Species 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Polymers 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 206010066901 Treatment failure Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- QVMHUALAQYRRBM-UHFFFAOYSA-N [P].[P] Chemical compound [P].[P] QVMHUALAQYRRBM-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- -1 antibiotic Chemical compound 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000003044 randomized block design Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000009340 sequential cropping Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of anti-disease microbial composite bacteria agents for preventing and treating tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof, belong to agricultural fertilizer production technical field.Anti-disease microbial microbial inoculum of the invention includes bacillus subtilis, bacillus amyloliquefaciens, streptomyces microflavus, Trichoderma viride, and total viable count of anti-disease microbial composite bacteria agent is 5 × 108cfu/g.Biological organic fertilizer of the invention is mainly made of the raw material of above-mentioned anti-disease microbial composite bacteria agent and following parts by weight: 5~10 parts of microbial fermentation inoculum, 70~75 parts of oil cake, 15~20 parts of stalk.Biological organic fertilizer no pollution to the environment of the invention, the effect for preventing and treating tobacco fungi soil-borne disease are preferable.
Description
Technical field
The present invention relates to a kind of anti-disease microbial composite bacteria agent, biological organic fertilizer and its systems for preventing and treating tobacco fungi soil-borne disease
Preparation Method belongs to agricultural fertilizer production technical field.
Background technique
Soil-borne disease is that a kind of pathogen survives in soil or native table, infringement soil neutralize the disease of aerial plant stem, it
Occur closely related with many factors such as the resistances of the propagation characteristic of pathogen, weather conditions, sequential cropping cultivation and kind.Tobacco is true
Bacterium soil-borne disease does culture medium with complicated soil environment, between 3 kinds of host, parasitic animal and plant and edaphon factors it is mutual according to
Based on the relationship of relying, and other plant disease is generally only based on the relation of interdependence between host and parasitic animal and plant, because
The prevention and treatment of this tobacco fungi soil-borne disease is relatively difficult.Pathogen usually infects tobacco root from soil, causes the crop root to be
To the disease of complete stool, result in significant economic losses.At present to the prevention and treatment of tobacco fungi soil-borne disease based on chemical prevention, but chemistry
Prevent and treat, medicament residue health nocuousness to human body big to the side effect of environment, the long-time service of chemical pesticide, pathogen
It is easy to develop drug resistance to it, thus leads to control efficiency decline even prevention and treatment failure.Shift of crops is prevention and treatment tobacco fungi soil
One of the effective ways of disease are passed, but since China has a large population and a few land, normal crop rotation measure be cannot achieve in production.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of anti-disease microbial compound bacteria for preventing and treating tobacco fungi soil-borne disease
Agent, the anti-disease microbial composite bacteria agent are from a wealth of sources, at low cost.
The present invention also provides a kind of biological organic fertilizers for preventing and treating tobacco fungi soil-borne disease, and the biological organic fertilizer is containing above-mentioned disease-resistant
Complex microbial inoculum can achieve the effect that prevent and treat tobacco fungi soil-borne disease well.
Finally, the present invention provides a kind of preparation method of biological organic fertilizer for preventing and treating tobacco fungi soil-borne disease, pass through the system
Biological organic fertilizer made from Preparation Method is at low cost, economic and environment-friendly.
To achieve the goals above, the technical scheme adopted by the invention is that:
A kind of anti-disease microbial composite bacteria agent for preventing and treating tobacco fungi soil-borne disease, including bacillus subtilis, solution starch bud
Spore bacillus, streptomyces microflavus, Trichoderma viride, bacillus subtilis, bacillus amyloliquefaciens, streptomyces microflavus, Trichoderma viride
The ratio between viable count is 2~3:5~6:1~2:1~2.
Bacillus subtilis and bacillus amyloliquefaciens can secrete chitinase, and chitinase can act on β-Isosorbide-5-Nitrae
Glycosidic bond decomposes chitin and generates chitin oligo saccharide, and chitin oligo saccharide can induce plant to generate disease-resistant factor, to inhibit pathogen
Invasion, furthermore bacillus subtilis and bacillus amyloliquefaciens can destroy fungal cell wall so as to fungi generate inhibit make
With.Its inhibiting effect to pathogen can be enhanced in bacillus subtilis and bacillus amyloliquefaciens synergistic effect.
Streptomyces microflavus is one kind of actinomycete streptomyces, contains auxin, antibiotic, benzene second in metabolite
Growth regulator ingredient necessary to the plant growths such as acid, succinic acid and the basic element of cell division has conversion the soil nitrogen, phosphorus phosphorus, potassium,
It increases soil fertility, reduces the effect of fertilizer amount；Additionally have and inhibit pathogen breeding, diseases prevention is kept a full stand of seedings, stimulation cell point
It splits, promotes crop take root, germinate, is mature, improving crop yield, promote active principle synthesis, significantly improve the quality of agricultural product
The effects of, it can reach the effect of " increasing both production and income is retained and supports field, inhibit disease, improve quality " quadruple effect is unified.Trichoderma viride passes through
It generates antibiotic, hyperparasitism, bacteriolysis, Competition etc. and antagonism work is generated to the pathogen of fungi type soil-borne disease
With.
Disease-resistant probiotics can produce the effects of antibiotic, growth hormone in pathogen, and reaching improves soil texture, improves
Soil fertility, the purpose for inhibiting disease.
Pass through beneficial bacterium bacillus subtilis, the phase of bacillus amyloliquefaciens and Antagonistic Fungi streptomyces microflavus, Trichoderma viride
Mutually synergistic effect enhances the effect for inhibiting pathogen, while improving the diversity of Soil Microorganism, makes soil micro-ecosystem
Reach balance, and then enhance the absorption and utilization efficiency of tobacco root nutrient, improves root activity.
Total viable count of above-mentioned anti-disease microbial composite bacteria agent is 5 × 108cfu/g.Anti-disease microbial composite bacteria agent is always lived
Bacterium number is 5 × 108It inhibits the effect of pathogen more prominent when cfu/g, and protection effect is more preferable.
A kind of biological organic fertilizer for preventing and treating tobacco fungi soil-borne disease, mainly by anti-disease microbial composite bacteria agent and following weight
The raw material of number is made: 5~8 parts of microbial fermentation inoculum, 70~75 parts of oil cake, 15~20 parts of stalk, the anti-disease microbial
Composite bacteria agent includes bacillus subtilis, bacillus amyloliquefaciens, streptomyces microflavus, Trichoderma viride, bacillus subtilis, Xie Dian
The ratio between afnyloliquefaciens, streptomyces microflavus, viable count of Trichoderma viride are 2~3:5~6:1~2:1~2.
Composite bacteria agent containing anti-disease microbial in the biological organic fertilizer, in addition, microelement rich in oil cake, disease-resistant
Complex microbial inoculum and oil cake, stalk, which are used in mixed way, both meets cigarette strain to nutrition temperature requirements, also provides for tobacco rhizosphere
Good micro-ecological environment, achievees the effect that controlling disease.
The microbial fermentation inoculum is bacillus subtilis, aspergillus niger, saccharomycete, the viable count of microbial fermentation inoculum
It is 5 × 107cfu/g.5 × 10 are added in biological organic fertilizer7The microbial fermentation inoculum of cfu/g viable count, can quickly by
Oil cake, Straw decomposing.
The preparation method of the biological organic fertilizer of above-mentioned prevention and treatment tobacco fungi soil-borne disease, comprising the following steps:
(1) water is added after mixing oil cake and stalk, mixes to obtain mixture；
(2) it is added into mixture after microbial fermentation inoculum mixes and carries out the decomposed material that ferments to obtain；
(3) into decomposed material be added anti-disease microbial composite bacteria agent mix fermentation to get.
The preparation method simple process, it is easy to accomplish, it is biological organic fertilizer no pollution to the environment obtained, at low cost.
The quality of water described in step (1) accounts for the 55~65% of total weight of the mixture.It is added in raw material under suitable water is
The aerobic fermentation of one step provides advantage, and the efficiency of aerobic fermentation can be improved.
Fermentation described in step (2) is aerobic fermentation, and the temperature of aerobic fermentation is 40~65 DEG C, and the time of aerobic fermentation is
15~40 days.The strain in anti-disease microbial composite bacteria agent in step (2) belongs to aerobic microorganism, in 40~65 DEG C of temperature
Lower fermentation can make within 15~40 days fermenting raw materials more sufficiently, decompose it is more thorough.
Fermentation described in step (3) is aerobic fermentation, and the temperature of aerobic fermentation is 30~40 DEG C, and the time of aerobic fermentation is
5~7 days.The fermentation condition can make total viable count content of anti-disease microbial in biological organic fertilizer maintain higher level.
Specific embodiment
The present invention is further explained in the light of specific embodiments.
The number of each raw material is parts by weight in following example；In following example in anti-disease microbial composite bacteria agent
Bacillus amyloliquefaciens, bacillus subtilis, streptomyces microflavus, Trichoderma viride strain are purchased from the limited public affairs of Yiyuan Kang Yuan biotechnology
It takes charge of (streptomyces microflavus article No. 4.115, Trichoderma viride article No. 3.140)；Microbial fermentation inoculum moistens field biotechnology purchased from Hebi
Co., Ltd；Conventional organic fertilizer is purchased from Wuzhoufeng Agricultural Technology Co., Ltd..
The embodiment 1 for preventing and treating the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease
The present embodiment prevents and treats the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, by bacillus subtilis, solution starch
Bacillus, streptomyces microflavus, Trichoderma viride composition, total viable count of anti-disease microbial composite bacteria agent are 5 × 108Cfu/g is withered
The ratio between careless bacillus, bacillus amyloliquefaciens, streptomyces microflavus, viable count of Trichoderma viride are 2:5:1:2.
The embodiment 2 for preventing and treating the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease
The present embodiment prevents and treats the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, by bacillus subtilis, solution starch
Bacillus, streptomyces microflavus, Trichoderma viride composition, total viable count of anti-disease microbial composite bacteria agent are 5 × 108Cfu/g is withered
The ratio between careless bacillus, bacillus amyloliquefaciens, streptomyces microflavus, viable count of Trichoderma viride are 1:6:1:2.
The embodiment 3 for preventing and treating the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease
The present embodiment prevents and treats the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, by bacillus subtilis, solution starch
Bacillus, streptomyces microflavus, Trichoderma viride composition, total viable count of anti-disease microbial composite bacteria agent are 5 × 108Cfu/g is withered
The ratio between careless bacillus, bacillus amyloliquefaciens, streptomyces microflavus, viable count of Trichoderma viride are 1:5:2:2.
The embodiment 1 for preventing and treating the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment prevents and treats the biological organic fertilizer of tobacco fungi soil-borne disease, mainly by anti-disease microbial composite bacteria agent and following
The raw material of parts by weight is made: 7 parts of microbial fermentation inoculum, 73 parts of oil cake, 15 parts of stalk.The microbial fermentation inoculum is withered
Careless bacillus, aspergillus niger, saccharomycete, total viable count of microbial fermentation inoculum are 5 × 107Cfu/g, anti-disease microbial are compound
Microbial inoculum is the anti-disease microbial composite bacteria agent in the embodiment 1 for prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease.
The embodiment 2 for preventing and treating the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment prevents and treats the biological organic fertilizer of tobacco fungi soil-borne disease, mainly by anti-disease microbial composite bacteria agent and following
The raw material of parts by weight is made: 10 parts of microbial fermentation inoculum, 70 parts of oil cake, 20 parts of stalk.The microbial fermentation inoculum is
Bacillus subtilis, aspergillus niger, saccharomycete, total viable count of microbial fermentation inoculum are 5 × 107Cfu/g, anti-disease microbial are multiple
Combined bacteria agent is the anti-disease microbial compound bacteria in the embodiment 2 for prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease
Agent.
The embodiment 3 for preventing and treating the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment prevents and treats the biological organic fertilizer of tobacco fungi soil-borne disease, mainly by anti-disease microbial composite bacteria agent and following
The raw material of parts by weight is made: 7 parts of microbial fermentation inoculum, 75 parts of oil cake, 18 parts of stalk.The microbial fermentation inoculum is withered
Careless bacillus, aspergillus niger, saccharomycete, total viable count of microbial fermentation inoculum are 5 × 107Cfu/g, anti-disease microbial are compound
Microbial inoculum is the anti-disease microbial composite bacteria agent in the embodiment 3 for prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease.
The embodiment 1 for preventing and treating the preparation method of the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment is the preparation of the biological organic fertilizer in the embodiment 1 for prevent and treat the biological organic fertilizer of tobacco fungi soil-borne disease
Method, comprising the following steps:
(1) water is added after the grouts oil cake and straw powder mixing after vegetable seed extracting oil, mixes well to obtain mixture, the matter of water
Amount accounts for the 56% of total weight of the mixture；
(2) microbial fermentation inoculum is added into mixture to be sufficiently mixed；Then high 1.3 meters, 4~5 meters long, width are piled
2.5 meters of heap body carries out aerobic fermentation, and fermentation temperature is 55 DEG C, and fermentation time is 40 days (winter in spring)；Fermentation temperature is down to
Stop fermentation when room temperature, obtains decomposed material；
(3) decomposed material is smashed it through into 50 meshes with pulverizer, adds anti-disease microbial composite bacteria agent, be uniformly mixed
After continue fermentation 7 days to obtain the final product.
The embodiment 2 for preventing and treating the preparation method of the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment is the preparation of the biological organic fertilizer in the embodiment 2 for prevent and treat the biological organic fertilizer of tobacco fungi soil-borne disease
Method, comprising the following steps:
(1) water is added after the grouts oil cake and straw powder mixing after vegetable seed extracting oil, mixes well to obtain mixture, the matter of water
Amount accounts for the 60% of total weight of the mixture；
(2) microbial fermentation inoculum is added into mixture to be sufficiently mixed；Then it piles high at 1.3 meters, 4~5 meters long, width
2.5 meters of heap body carries out aerobic fermentation, and fermentation temperature is 65 DEG C, and fermentation time is 17 days (summer and autumn)；Fermentation temperature is down to
Stop fermentation when room temperature, obtains decomposed material；
(3) decomposed material is smashed it through into 50 meshes with pulverizer, adds anti-disease microbial composite bacteria agent, be uniformly mixed
After continue fermentation 7 days to obtain the final product.
The embodiment 3 for preventing and treating the preparation method of the biological organic fertilizer of tobacco fungi soil-borne disease
The present embodiment is the preparation of the biological organic fertilizer in the embodiment 3 for prevent and treat the biological organic fertilizer of tobacco fungi soil-borne disease
Method, comprising the following steps:
(1) water is added after the grouts oil cake and straw powder mixing after vegetable seed extracting oil, mixes well to obtain mixture, the matter of water
Amount accounts for the 65% of total weight of the mixture；
(2) microbial fermentation inoculum is added into mixture to be sufficiently mixed；Then it piles high at 1.3 meters, 4~5 meters long, width
2.5 meters of heap body carries out aerobic fermentation, and fermentation temperature is 60 DEG C, and fermentation time is 20 days (summer and autumn)；Fermentation temperature is down to
Stop fermentation when room temperature, obtains decomposed material；
(3) decomposed material is smashed it through into 50 meshes with pulverizer, adds anti-disease microbial composite bacteria agent, be uniformly mixed
After continue fermentation 7 days to obtain the final product.
Test example 1
This test example choose prevention and treatment tobacco fungi soil-borne disease biological organic fertilizer embodiment 2 in biological organic fertilizer be
Example, investigates biological organic fertilizer of the invention to the protection effect of flue-cured tobacco balck shank and root rot.
It promotes test and is carried out in Liangshan State of Sichuan Province Puge County, Dechang County, Mianning County demonstration area in crop field.Field experiment is chosen
Fertility is relatively uniform, topography is flat, the plot of previous high-incidence fungi soil-borne disease is experimental field, transplants tobacco seedlings at the end of April.Test according to
According to RANDOMIZED BLOCK DESIGN, crop field amounts to 6 cells, and every cell plants 200 plants of tobacco seedlings, and plant spacing is 1100 plants/
666.67m2, plant/line spacing is 0.5m × 1.1m, area of field 1236.34m2, daily field management is according to High Quality Tobacco cultivation skill
Art carries out.
2 processing of test setting, each processing are repeated 3 times.Control group presses local conventional fertilizer application mode and quantity carries out, and applies
Add organic fertilizer, applied amount 50kg/666.67m2；Test group applies the biological organic fertilizer of the invention for controlling tobacco fungi soil-borne disease
Biological organic fertilizer in embodiment 2, applied amount 50kg/666.67m2。
Prosperous growth phase and maturity period respectively investigate the cigarette strain balck shank and root rot in Puge County experimental plot, as a result as follows
Shown in table 1, the control efficiency of test group is preferable as can be seen from Table 1, and it is black that biological organic fertilizer of the invention either prevents and treats tobacco
Shin disease or root rot, effect are superior to conventional organic fertilizer.
1 Puge County different disposal cigarette strain balck shank of table and root rot control efficiency compare
Prosperous growth phase and maturity period carry out the investigation of balck shank and root rot to the cigarette strain in Dechang County experimental plot respectively, as a result such as
Shown in the following table 2, the disease incidence of test group balck shank and root rot is all significantly lower than control group as can be seen from Table 2, always relatively anti-
Effect up to 72% or more.
2 Dechang County different disposal cigarette strain balck shank of table and root rot control efficiency compare
Prosperous growth phase and maturity period carry out the investigation of balck shank and root rot to the cigarette strain in Mianning County experimental plot, as a result such as following table
Shown in 3, from table 3 it can be seen that the relative control effect of prosperous growth phase cigarette strain balck shank and root rot has respectively reached 57.1% He
57.9%, and the relative control effect in maturity period is above 70%.Illustrate to apply biological organic fertilizer treated tobacco black shank and root
The state of an illness of maize ear rot is controlled effectively and alleviates.
3 Mianning County different disposal cigarette strain balck shank of table and root rot control efficiency compare
Test example 2
This test example carries out economic characters assessment to the cigarette strain in Puge County and Mianning County experimental plot in test example 1, as a result such as
Shown in the following table 4, table 5.From table 4, it can be seen that in the experimental plot of Puge County, in test group cigarette strain equal cigarettes ratio and medium grade cigarette ratio compared with
Greatly, the output value increases by 195 yuan per acre.Test group per mu yield in Mianning County is 1.24kg higher than control group as can be seen from Table 5, average per acre
The output value increases 84.65 yuan, illustrates after applying biological organic fertilizer of the invention, and per mu yield, the output value of cigarette strain have in various degree
Raising.
4 Puge County of table respectively handles the influence to cigarette strain economic characters
5 Mianning of table respectively handles the influence to tobacco leaf economic characters
Claims (8)
1. it is a kind of prevent and treat tobacco fungi soil-borne disease anti-disease microbial composite bacteria agent, it is characterised in that: including bacillus subtilis,
Bacillus amyloliquefaciens, streptomyces microflavus, Trichoderma viride are bacillus subtilis, bacillus amyloliquefaciens, streptomyces microflavus, green
The ratio between viable count of color trichoderma is 2~3:5~6:1~2:1~2.
2. the anti-disease microbial composite bacteria agent of prevention and treatment tobacco fungi soil-borne disease according to claim 1, it is characterised in that: institute
The total viable count for stating anti-disease microbial composite bacteria agent is 5 × 108cfu/g。
3. a kind of biological organic fertilizer for preventing and treating tobacco fungi soil-borne disease, it is characterised in that: mainly by anti-disease microbial composite bacteria agent
It is made with the raw material of following parts by weight: 5~8 parts of microbial fermentation inoculum, 70~75 parts of oil cake, 15~20 parts of stalk, it is described
Anti-disease microbial composite bacteria agent includes bacillus subtilis, bacillus amyloliquefaciens, streptomyces microflavus, Trichoderma viride, withered grass bud
The ratio between spore bacillus, bacillus amyloliquefaciens, streptomyces microflavus, viable count of Trichoderma viride are 2~3:5~6:1~2:1~2.
4. the biological organic fertilizer of prevention and treatment tobacco fungi soil-borne disease according to claim 3, it is characterised in that: the microorganism
Fermenting agent is bacillus subtilis, aspergillus niger, saccharomycete, and the viable count of microbial fermentation inoculum is 5 × 107cfu/g。
5. the preparation method of the biological organic fertilizer of prevention and treatment tobacco fungi soil-borne disease as claimed in claim 3, it is characterised in that: packet
Include following steps:
(1) water is added after mixing oil cake and stalk, mixes to obtain mixture；
(2) it is added into mixture after microbial fermentation inoculum mixes and carries out the decomposed material that ferments to obtain；
(3) into decomposed material be added anti-disease microbial composite bacteria agent mix fermentation to get.
6. the preparation method of the biological organic fertilizer of prevention and treatment tobacco fungi soil-borne disease according to claim 5, it is characterised in that:
The quality of water described in step (1) accounts for the 55~65% of total weight of the mixture.
7. the preparation method of the biological organic fertilizer of prevention and treatment tobacco fungi soil-borne disease according to claim 5, it is characterised in that:
Fermentation described in step (2) is aerobic fermentation, and the temperature of aerobic fermentation is 40~65 DEG C, and the time of aerobic fermentation is 15~40
It.
8. the preparation method of the biological organic fertilizer of prevention and treatment tobacco fungi soil-borne disease according to claim 5, it is characterised in that:
Fermentation described in step (3) is aerobic fermentation, and the temperature of aerobic fermentation is 30~40 DEG C, and the time of aerobic fermentation is 5~7 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811378223.6A CN109402012B (en) | 2018-11-19 | 2018-11-19 | Disease-resistant microbial compound microbial agent for preventing and treating soil-borne diseases of tobacco fungi, biological organic fertilizer and preparation method of microbial compound microbial agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811378223.6A CN109402012B (en) | 2018-11-19 | 2018-11-19 | Disease-resistant microbial compound microbial agent for preventing and treating soil-borne diseases of tobacco fungi, biological organic fertilizer and preparation method of microbial compound microbial agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109402012A true CN109402012A (en) | 2019-03-01 |
| CN109402012B CN109402012B (en) | 2020-10-30 |
Family
ID=65474154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811378223.6A Expired - Fee Related CN109402012B (en) | 2018-11-19 | 2018-11-19 | Disease-resistant microbial compound microbial agent for preventing and treating soil-borne diseases of tobacco fungi, biological organic fertilizer and preparation method of microbial compound microbial agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109402012B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111393211A (en) * | 2020-03-16 | 2020-07-10 | 中国烟草总公司海南省公司 | Special biological organic fertilizer for cigar outer wrapper tobacco leaves and preparation method and application thereof |
| CN112341284A (en) * | 2020-11-24 | 2021-02-09 | 商洛学院 | Special microbial fertilizer for tobacco and preparation method and application thereof |
| CN112522149A (en) * | 2020-12-11 | 2021-03-19 | 湖南稼陶农业科技有限公司 | Compound microbial agent capable of preventing and treating tobacco diseases |
| CN113248326A (en) * | 2021-05-20 | 2021-08-13 | 凉山金叶化肥有限责任公司 | Organic fertilizer for cigarettes and preparation method thereof |
| CN114058546A (en) * | 2021-11-25 | 2022-02-18 | 山东蔚蓝绿源生物科技有限公司 | Composite microbial inoculum for preventing and treating plant diseases and application thereof |
| CN114395397A (en) * | 2022-01-27 | 2022-04-26 | 云南农业大学 | Microbial soil improvement microbial inoculum for preventing and treating tobacco blight and preparation method thereof |
| CN115322907A (en) * | 2022-05-16 | 2022-11-11 | 湖南中烟工业有限责任公司 | Microbial agent with function of preventing and controlling tobacco root rot and application thereof |
| CN115433029A (en) * | 2022-10-08 | 2022-12-06 | 安徽正基生态农业有限公司 | Special biological fertilizer for preventing and treating sweet potato black spot diseases and insect pests and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105036986A (en) * | 2015-08-17 | 2015-11-11 | 苏州玖沃生物科技有限公司 | Microbial fertilizer resistant to diseases and insects and preparation method thereof |
| CN105753607A (en) * | 2016-03-29 | 2016-07-13 | 张乙航 | Biological agent for inhibiting spreading of diseases and pests in soil |
| CN106497831A (en) * | 2016-10-21 | 2017-03-15 | 广州市林业和园林科学研究院 | A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application |
| CN107384825A (en) * | 2017-08-09 | 2017-11-24 | 郑州大学 | Agricultural microorganism preparation and its application in terms of vega continuous cropping obstacle is overcome |
-
2018
- 2018-11-19 CN CN201811378223.6A patent/CN109402012B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105036986A (en) * | 2015-08-17 | 2015-11-11 | 苏州玖沃生物科技有限公司 | Microbial fertilizer resistant to diseases and insects and preparation method thereof |
| CN105753607A (en) * | 2016-03-29 | 2016-07-13 | 张乙航 | Biological agent for inhibiting spreading of diseases and pests in soil |
| CN106497831A (en) * | 2016-10-21 | 2017-03-15 | 广州市林业和园林科学研究院 | A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application |
| CN107384825A (en) * | 2017-08-09 | 2017-11-24 | 郑州大学 | Agricultural microorganism preparation and its application in terms of vega continuous cropping obstacle is overcome |
Non-Patent Citations (1)
| Title |
|---|
| 韩长志: ""植物病害生防菌的研究现状及发展趋势"", 《中国森林病虫》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111393211A (en) * | 2020-03-16 | 2020-07-10 | 中国烟草总公司海南省公司 | Special biological organic fertilizer for cigar outer wrapper tobacco leaves and preparation method and application thereof |
| CN112341284A (en) * | 2020-11-24 | 2021-02-09 | 商洛学院 | Special microbial fertilizer for tobacco and preparation method and application thereof |
| CN112522149A (en) * | 2020-12-11 | 2021-03-19 | 湖南稼陶农业科技有限公司 | Compound microbial agent capable of preventing and treating tobacco diseases |
| CN113248326A (en) * | 2021-05-20 | 2021-08-13 | 凉山金叶化肥有限责任公司 | Organic fertilizer for cigarettes and preparation method thereof |
| CN114058546A (en) * | 2021-11-25 | 2022-02-18 | 山东蔚蓝绿源生物科技有限公司 | Composite microbial inoculum for preventing and treating plant diseases and application thereof |
| CN114058546B (en) * | 2021-11-25 | 2022-08-05 | 山东蔚蓝绿源生物科技有限公司 | Composite microbial inoculum for preventing and treating plant diseases and application thereof |
| CN114395397A (en) * | 2022-01-27 | 2022-04-26 | 云南农业大学 | Microbial soil improvement microbial inoculum for preventing and treating tobacco blight and preparation method thereof |
| CN115322907A (en) * | 2022-05-16 | 2022-11-11 | 湖南中烟工业有限责任公司 | Microbial agent with function of preventing and controlling tobacco root rot and application thereof |
| CN115322907B (en) * | 2022-05-16 | 2023-04-18 | 湖南中烟工业有限责任公司 | Microbial agent with function of preventing and controlling tobacco root rot and application thereof |
| CN115433029A (en) * | 2022-10-08 | 2022-12-06 | 安徽正基生态农业有限公司 | Special biological fertilizer for preventing and treating sweet potato black spot diseases and insect pests and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109402012B (en) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109402012A (en) | It is a kind of to prevent and treat the anti-disease microbial composite bacteria agent of tobacco fungi soil-borne disease, biological organic fertilizer and preparation method thereof | |
| CN104177137B (en) | A kind of microbial straw composing agent and preparation method thereof | |
| CN102515951B (en) | Tobacco composite microbial fertilizer and its preparation method | |
| CN101126026B (en) | Vegetable field soil improving agent and its preparation method | |
| CN103274825B (en) | A kind of polynary microorganism special fertilizer preventing and treating celery soft rot and preparation method thereof | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN103819237A (en) | Microbial fertilizer special for tobaccos, and preparing method thereof | |
| CN103483020A (en) | Bio-organic fertilizer and method for preparing bio-organic fertilizer which prevents and treats soil insects | |
| CN103274843B (en) | A kind of polynary microorganism special fertilizer preventing and treating bacterial soft rot of potato and preparation method thereof | |
| CN106554240A (en) | A kind of crops soil improving agent for one-crop succession and preparation method thereof | |
| CN1398824A (en) | Bioorganic fertilizer and its production process | |
| CN106278692A (en) | A kind of acidic soil conditioner and preparation method thereof | |
| CN103274836B (en) | A kind of polynary microorganism special fertilizer preventing and treating numb Chinese yam anthrax and preparation method thereof | |
| CN105367217A (en) | Compound microorganism humic acid fertilizer special for organic cultivation and preparation method thereof | |
| CN102523879A (en) | Method for improving soil for nonforest land planting of Panax ginseng by green fallowing | |
| CN104447127A (en) | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof | |
| CN104099281A (en) | Environment-friendly compound microbial agent | |
| CN104099282B (en) | Environment-friendly stalk degradation agents | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN103274834A (en) | Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof | |
| CN107445694A (en) | A kind of composite bio-chemical preparation and its applied in straw-returning | |
| CN107721713A (en) | A kind of microbial compound bacterial fertilizer and preparation method and application | |
| CN106747737A (en) | A kind of special organic bio-fertilizer of the bletilla striata and its preparation technology | |
| CN106380269A (en) | Soil remediation organic fertilizer for overcoming watermelon replantation obstacle, preparation method thereof and application thereof | |
| CN106007824A (en) | Compound bacterial fertilizer as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201030 |