CN106497831A - A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application - Google Patents
A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to field of plant growing technology, and in particular to a kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation method and application.The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, mainly by being made with hypothallus：Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria.A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the present invention, it is used as Antagonistic Fungi by choosing streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are chosen as trophic function bacterium, so which forms certain antagonism to Phytophthora nicotianae disease pathogen, can more effectively prevent the injury of Phytophthora nicotianae disease.A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the present invention, directly plant growing can use, it is also possible to be applied to be inoculated with compost, produce biological organic fertilizer.
Description
Technical field
The present invention relates to field of plant growing technology, and in particular to a kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable and its preparation
Methods and applications.
Background technology
Phytophthora is the important phytopathogen of a group, and, to the destructive strong of host plant, hazardness is big for the bacterium.With me
The change of state's structure of agricultural production, a large amount of development of fruit tree, vegetable, flowers, Chinese medicinal herbses and industrialized agriculture, vegetable plague pair
The threat of agricultural production is on the rise.Herba Catharanthi Rosei epidemic disease pathogen is Nicotiana tabacum L. epidemic disease, and 28 DEG C of temperature, relative air humidity are higher than
85% is the optimal condition of the sick happening and prevelence.Flower, stem, leaf can be aggrieved, and the nascent taupe oil stain shape stigma in sick portion, after extension
Irregularly shaped in pitchy, the life sparse mould layer of white in disease portion under wet condition, when falling ill serious stem be folded down vertical, blade with
The water stain shape brown maculeses of aggrieved rear appearance are spent, and perishable.As phytophthora is that a kind of soil passes bacterium, belong to oomycetes in classification,
With funguses wide apart on evolutionary relationship, most of conventional antifungal is invalid to phytophthora, causes vegetable plague more difficult anti-
Control, it usually needs take the aggregate measures such as agricultural measures, disease-resistant variety, chemical prevention obtain preferable prevention effect, and
Biological control then more meets requirement of the people to food and Environmental security.
1）The agricultural measures such as agricultural measures and disease-resistant variety selection-breeding, popularization crop rotation, adjustment crop sowing time can mitigate bacterium
Source, reduces disease and occurs, be effectively to prevent and treat the pathogenetic method of epidemic disease, be most economical effective side wherein using plant resistance to environment stress
Method, to some important plant (such as Rhizoma Solani tuber osis), should strengthen varietal resistance evaluation identification, the selection-breeding of disease-resistant variety and popularization；
2）Strengthen field or plantation management field or plantation humidity, water-holding capacity and plant density directly affect sending out for epidemic disease
Raw and popular, therefore, prepartion of land, timely draining, appropriate adjustment plant density is carried out to field or plantation wet so as to reduce
Degree is conducive to mitigating epidemic disease occurrence degree.Additionally, the rational application of fertilizer, strengthens the resistances against diseases of plant, it is also beneficial to mitigate the state of an illness；
3）Chemical prevention epidemic disease is also the most frequently used prophylactico-therapeutic measuress at present, and benzamideses antibacterial (PAFs) is the special of preventing and treating phytophthora
Use medicament.The upper preventive effect of production at present is preferable, apply more pesticide to have smart first frost MnZn WP of 25% Jiameiling WP and 68% etc.,
Spray.Due to benzamideses antibacterial action site single, it is easy to make pathogen undergo mutation,
Develop immunity to drugs, it is therefore desirable to rationally pacify for different regions phytophthora (biological strain) distribution, Drug resistance, pathogenicity feature
Row uses chemical pesticide, it is to avoid the long-time of single pesticide is used, in case the generation of Drug resistance and cross resistance；
Plant disease biocontrol microorganisms have been directed to funguses, actinomycetes, antibacterial or even virus（Phage）, Biological control is using a kind of
Or multiple biological or its metabolite mitigate plant disease reduce the quantity of the phytopathy original or suppressing its pathogenecity
One class measure, by the beneficial microbe group of plant, pathogen, biocontrol microorganisms, plant surface and its surrounding and natural environment etc.
Factor interaction each other, reaches the effect of growth-promoting diseases prevention.Cause the cause of disease species of plant disease a lot, including
Funguses, antibacterial, nematicide, virus etc..They generally infect the root of plant, cause the root even disease of Herb, so as to cause
Great economic loss.Antagonistic Fungi mainly has bacillus cereuss, Pseudomonas alba and streptomycete etc., and bacillus cereuss are current biocontrol microorganisms
The more class of research, because which is capable of the endospore of heat-resisting, drought-enduring, uvioresistant and organic solvent, is preferable biocontrol microorganisms
Screening object.The scope of restraining fungi of bacillus cereuss is very wide, including root disease, branch disease, leaf, flower portion disease and fruit disease.
The bacillus cereuss for having antagonism that has reported have bacillus subtilises, Bacillus cercuses, bacillus polymyxa, huge spore bar
Bacterium, Bacillus pumilus etc..Streptomycete is the main production bacterium of antibiotic agents, and more than 20000 kinds produced in microorganism are lived
In property material, streptomycete accounts for more than 60%, is class crop probioticss.Streptomyces microflavus are used as a kind in streptomyces
Class, under natural cultivation condition, its own metabolite can be effectively facilitated plant growing, be widely used to fruit tree, vegetable
Cultivate etc. various plants, and there is obvious growth-promoting, resistant effect to crops such as vegetables；
4）Biological control is the result of pathogen and the interphase interaction of antagonistic microbe.Antagonistic microbe suppresses disease by producing
The antibiotic of the growth of opportunistic pathogen, spore germination or Sporulation or poisonous metabolite, direct parasitism pathogen and pathogen
The mode controlling disease such as nutrient competition.Whether which kind of suppresses distress mechanism, and the quantity or activity of Antagonistic Fungi all must be maintained at
Suppress the threshold level of disease, if the quantity of Antagonistic Fungi or activity are less than this threshold value, then Phytophthora nicotianae Breda may develop
And cause disease.Research shows, for the soil of Phytophthora nicotianae is suppressed, increases quantity of useful microbe and advantageously reduces disease
Evil incidence rate or seriousness, and these quantity of useful microbe and activity are that the organic substance of Nutrition is tieed up by soil
Hold.In the critical period of morbidity, active antagonistic microbe can effectively hinder the development of pathogen, so as to the generation of controlling disease.
However, in recent years due to being impacted by fertilizer efficiency, there is tobacco grower's Shaoshi fertilizer, how phenomenon to fertilize, cause soil
The earth content of organic matter declines and Antagonistic Fungi normally cannot be bred, thus is difficult large area and is prevented and treated.Biological control is with people
Concern to food and environmental security, Biological control will be expected to more and more be paid attention to and tremendous development.
Content of the invention
Present invention aims to deficiency of the prior art, and a kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable is provided
And its preparation method and application, can apply to prevent and treat Phytophthora nicotianae using preventing and treating Phytophthora nicotianae disease composite bacteria agent capable obtained in the method
Disease.
For achieving the above object, the technical scheme taken of the present invention is：A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, mainly
By being made with hypothallus：
Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria.
Preferably, the photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are single bacterium colony thalline.
The present invention also provides the preparation method of above-mentioned preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, comprises the following steps：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking photosynthetic bacteria is inoculated into yeast extract fluid medium
In；The incubation step of picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria is added in Rhizoma Solani tuber osi fluid medium；Each training
In 20-35 DEG C of condition bottom fermentation 24-96h, foster base treats that thalline content is higher than 10 in each culture medium8Cfu/ml stops fermenting, respectively
Culture medium is in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, each bacterium solution is mixed by equal-volume ratio, prepared described multiple
Close microbial inoculum.
Preferably, in 28 DEG C of condition bottom fermentation 72h, each culture medium treats that thalline content is higher than 10 in each culture medium8cfu/
Ml stops fermenting, and each culture medium is in 150r min-1Under the conditions of centrifugation obtain each bacterium solution, by each bacterium solution by equal-volume ratio mixing,
The composite bacteria agent capable is obtained.
As the preferred implementation of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the present invention, the preventing and treating Phytophthora nicotianae disease
Composite bacteria agent capable also includes bacillus subtilises and bacillus megaterium.
As the preferred implementation of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the present invention, the preventing and treating Phytophthora nicotianae disease
Composite bacteria agent capable also includes Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma.
As of the present invention preventing and treating Phytophthora nicotianae disease composite bacteria agent capable preferred implementation, the bacillus subtilises,
Bacillus megaterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma are single bacterium colony thalline.
The present invention also provides the preparation method of above-mentioned preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, comprises the following steps：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking streptomyces microflavus add Gause I liquid culture
In base；Picking photosynthetic bacteria is inoculated in yeast extract fluid medium；Picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria
Incubation step add Rhizoma Solani tuber osi fluid medium in；Picking bacillus subtilises, bacillus megaterium single bacterium colony are separately added into
In beef extract-peptone fluid medium；Picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma, proteolysis candida mycoderma single bacterium colony point
Jia Ru not be in PDA liquid medium；In 20-35 DEG C of condition bottom fermentation 24-96h, each culture medium treats that thalline contains in each culture medium
Amount is higher than 108Cfu/ml stops fermenting, and each culture medium is in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, by each bacterium
Liquid is mixed by equal-volume ratio, and the composite bacteria agent capable is obtained.
Preferably, in 28 DEG C of condition bottom fermentation 72h, each culture medium treats that thalline content is higher than 10 in each culture medium8cfu/
Ml stops fermenting, and each culture medium is in 150r min-1Under the conditions of centrifugation obtain each bacterium solution, by each bacterium solution by equal-volume ratio mixing,
The composite bacteria agent capable is obtained.
Finally, the present invention also provides application of the above-mentioned preventing and treating Phytophthora nicotianae disease composite bacteria agent capable in preventing and treating Phytophthora nicotianae disease.
Used as the preferred implementation of application of the present invention, the composite bacteria agent capable is applied to the Nicotiana tabacum L. epidemic disease for preventing and treating Herba Catharanthi Rosei
Mildew.
Beneficial effects of the present invention：
A kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the present invention, is used as Antagonistic Fungi by choosing streptomyces microflavus, chooses photosynthetic
Antibacterial, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are used as trophic function bacterium so which is to Phytophthora nicotianae disease pathogen
Certain antagonism is formed, can effectively prevent the injury of Phytophthora nicotianae disease.A kind of preventing and treating Phytophthora nicotianae disease of the present invention is multiple
Microbial inoculum is closed, directly plant growing can be used, it is also possible to be applied to be inoculated with compost, produce biological organic fertilizer.
Specific embodiment
The invention will be further described with the following Examples.
The photosynthetic bacteria of the embodiment of the present invention, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilises
(Bacillus subtilis), Trichoderma viride (Trichodermaviride), bacillus megaterium (Bacillus
Magaterium), saccharomyces cerevisiae (Saccharomyces revisiae), candida mycoderma (Candida tropicalis), solution
Protein candida mycoderma (Candida famata) is provided by Agricultural University Of South China's new fertilizer the Study on Resources center, and mainly material is rotten
Beneficial microbe that is ripe, promoting plant growing.
The streptomyces microflavus of the embodiment of the present invention, referred to as 5406, buy in Guangdong Province's Culture Collection,
GIM4.52.
Embodiment 1
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, mainly by being made with hypothallus：
Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria, all thalline are single bacterium colony bacterium
Body.Certainly, the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable can also include that other Antagonistic Fungis, the present embodiment are not specifically limited.
The preparation method of the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, comprises the following steps：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking photosynthetic bacteria is inoculated into yeast extract fluid medium
In；The incubation step of picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria is added in Rhizoma Solani tuber osi fluid medium；Each training
In 25 DEG C of condition bottom fermentation 36h, foster base treats that thalline content is higher than 10 in each culture medium8Cfu/ml stops fermenting, each culture medium
In 140r min-1Under the conditions of centrifugation obtain each bacterium solution, each bacterium solution is mixed by equal-volume ratio, the prepared composite bacteria agent capable.
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention can apply to the Phytophthora nicotianae disease for preventing and treating Herba Catharanthi Rosei.
Embodiment 2
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, mainly by being made with hypothallus：
Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilises and huge spore
Bacillus, all thalline are single bacterium colony thalline.Certainly, the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable can also include other antagonisms
Bacterium, the present embodiment are not specifically limited.
The preparation method of the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, comprises the following steps：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking streptomyces microflavus add Gause I liquid culture
In base；Picking photosynthetic bacteria is inoculated in yeast extract fluid medium；Picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria
Incubation step add Rhizoma Solani tuber osi fluid medium in；Picking bacillus subtilises, bacillus megaterium single bacterium colony are separately added into
In beef extract-peptone fluid medium；In 28 DEG C of condition bottom fermentation 72h, each culture medium treats that thalline content is high in each culture medium
In 108Cfu/ml stops fermenting, and each culture medium is in 150r min-1Under the conditions of centrifugation obtain each bacterium solution, by each bacterium solution by waiting body
Product is obtained the composite bacteria agent capable than mixing.
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention can apply to the Phytophthora nicotianae disease for preventing and treating Herba Catharanthi Rosei.
Embodiment 3
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, mainly by being made with hypothallus：
Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, bacillus subtilises, huge spore bar
Bacterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma, all thalline are single bacterium colony thalline.Certainly, described
Preventing and treating Phytophthora nicotianae disease composite bacteria agent capable can also include that other Antagonistic Fungis, the present embodiment are not specifically limited.
The preparation method of the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention, comprises the following steps：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking streptomyces microflavus add Gause I liquid culture
In base；Picking photosynthetic bacteria is inoculated in yeast extract fluid medium；Picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria
Incubation step add Rhizoma Solani tuber osi fluid medium in；Picking bacillus subtilises, bacillus megaterium single bacterium colony are separately added into
In beef extract-peptone fluid medium；Picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma, proteolysis candida mycoderma single bacterium colony point
Jia Ru not be in PDA liquid medium；In 33 DEG C of condition bottom fermentation 84h, each culture medium treats that thalline content is higher than in each culture medium
108Cfu/ml stops fermenting, and each culture medium is in 165r min-1Under the conditions of centrifugation obtain each bacterium solution, each bacterium solution is pressed equal-volume
Than mixing, the composite bacteria agent capable is obtained.
The preventing and treating Phytophthora nicotianae disease composite bacteria agent capable of the embodiment of the present invention can apply to the Phytophthora nicotianae disease for preventing and treating Herba Catharanthi Rosei.
Test example 1
First, the preparation of Phytophthora nicotianae disease pathogen：
1）Separate：Morbidity Herba Catharanthi Rosei picks up from Guangzhou white clouds main road greenbelt.Morbidity Herba Catharanthi Rosei stem is taken, is cut, add 70% wine
Smart surface is killed virus the 3-5 seconds, proceeds to 3% liquor natrii hypochloritises 3-5min, is put in aquesterilisa and cleans, uses sterilizing filter paper suck dry moisture,
PDA culture medium is put into, culture is inverted, is grown mycelia, choose and be put into other culture medium culturing.Its colonial morphology is observed, and is passed through
Make section, micro- sem observation.
2）Pathogenic：Take the normal Herba Catharanthi Rosei of growth, the basal part of stem knife that sterilizes gently is scratched, and pathogen is accessed, with going out
Bacterium filter paper is wrapped, moisturizing, not access pathogen as control.5 plant Herba Catharanthi Roseis are often processed, and incubated at room temperature observes its incidence,
Diseased plant is separated again, with clearly for the pathogenic of examination pathogen.As a result show, after the flower growth 4d of Changchun, be inoculated with pathogen
Process starts disease symptom occur, and black speck occurs in basal part of stem, and as time went on, basal part of stem is gradually downright bad, and gradually climing upwards
Prolong, the visible inside of vertical profile disease stem is in pitchy, and diseased plant blade turns yellow from bottom to top successively, wilts sagging, and final diseased plant is withered
Extremely.
2nd, test arranges 4 process, with the substrate of not inoculating compound bacterium agent as compareing CK, presses 1%, 3% and 5% respectively（Matter
Amount ratio）The substrate of the composite bacteria agent capable of the inoculation embodiment of the present invention 1 is followed successively by T1, T2 and T3.Per group processes 5 repetitions, fills per basin
Enter 250g substrate.Herba Catharanthi Rosei is sowed on April 12nd, 2016；May 12 moved into flowerpot, per 3 plants of seedling of pot transplanting；May 28
Inoculating compound bacterium agent.The growth of the observation of June 19 Herba Catharanthi Rosei and the incidence of Nicotiana tabacum L. epidemic disease.
From the point of view of Phytophthora nicotianae disease incidence, the morbidity strain number of the substrate plantation Herba Catharanthi Rosei of inoculating compound bacterium agent is not 2
Strain, presses 1%, 3% and 5% respectively（Mass ratio）The substrate plantation Herba Catharanthi Rosei of inoculating compound bacterium agent has no morbidity diseased plant.Be inoculated with this
There is the substrate plantation Herba Catharanthi Rosei of the composite bacteria agent capable of bright embodiment 1 effect of preventing and treating Phytophthora nicotianae disease, its reason on the one hand to originate
In the direct antagonism of Antagonistic Fungi, on the other hand from Ecological niche competition, the indirect action of nutrient competition.
Finally it should be noted that above example is only in order to illustrating technical scheme, rather than to present invention guarantor
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (9)
1. a kind of preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, it is characterised in that main by being made with hypothallus：
Streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria.
2. as claimed in claim 1 preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, it is characterised in that the photosynthetic bacteria, nitrobacteria,
Nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria are single bacterium colony thalline.
3. preventing and treating Phytophthora nicotianae disease composite bacteria agent capable as claimed in claim 1, it is characterised in that also include bacillus subtilises and huge
Bacterium anthracoides.
4. as claimed in claim 3 preventing and treating Phytophthora nicotianae disease composite bacteria agent capable, it is characterised in that also include Trichoderma viride, wine brewing ferment
Female, candida mycoderma and proteolysis candida mycoderma.
5. preventing and treating Phytophthora nicotianae disease composite bacteria agent capable as claimed in claim 4, it is characterised in that the bacillus subtilises, huge
Bacillus cereuss, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma are single bacterium colony thalline.
6. a kind of as claimed in claim 1 preventing and treating Phytophthora nicotianae disease composite bacteria agent capable preparation method, it is characterised in that including following
Step：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking photosynthetic bacteria is inoculated into yeast extract fluid medium
In；The incubation step of picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria is added in Rhizoma Solani tuber osi fluid medium；Each training
In 20-35 DEG C of condition bottom fermentation 24-96h, foster base treats that thalline content is higher than 10 in each culture medium8Cfu/ml stops fermenting, respectively
Culture medium is in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, each bacterium solution is mixed by equal-volume ratio, prepared described multiple
Close microbial inoculum.
7. a kind of as claimed in claim 4 preventing and treating Phytophthora nicotianae disease composite bacteria agent capable preparation method, it is characterised in that including following
Step：
Picking streptomyces microflavus are added in Gause I fluid medium；Picking streptomyces microflavus add Gause I liquid culture
In base；Picking photosynthetic bacteria is inoculated in yeast extract fluid medium；Picking nitrobacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria
Incubation step add Rhizoma Solani tuber osi fluid medium in；Picking bacillus subtilises, bacillus megaterium single bacterium colony are separately added into
In beef extract-peptone fluid medium；Picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma, proteolysis candida mycoderma single bacterium colony point
Jia Ru not be in PDA liquid medium；In 20-35 DEG C of condition bottom fermentation 24-96h, each culture medium treats that thalline contains in each culture medium
Amount is higher than 108Cfu/ml stops fermenting, and each culture medium is in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, by each bacterium
Liquid is mixed by equal-volume ratio, and the composite bacteria agent capable is obtained.
8. application of the preventing and treating Phytophthora nicotianae disease composite bacteria agent capable in preventing and treating Phytophthora nicotianae disease as described in any one of claim 1-5.
9. application as claimed in claim 8, it is characterised in that the composite bacteria agent capable is applied to the Phytophthora nicotianae for preventing and treating Herba Catharanthi Rosei
Disease.
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