CN108676748A - One plant of pear tree rhizosphere growth-promoting bacillus mycoides Lzh-Z7 and its application - Google Patents
One plant of pear tree rhizosphere growth-promoting bacillus mycoides Lzh-Z7 and its application Download PDFInfo
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- CN108676748A CN108676748A CN201810532646.2A CN201810532646A CN108676748A CN 108676748 A CN108676748 A CN 108676748A CN 201810532646 A CN201810532646 A CN 201810532646A CN 108676748 A CN108676748 A CN 108676748A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to one plant of pear tree rhizosphere growth-promoting bacillus mycoides Lzh Z7 and its applications, bacillus mycoides Lzh Z7 are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.13491, the deposit date is on December 23rd, 2016, preservation organization address was Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.The invention also includes bacillus mycoides Lzh Z7, zymotic fluid and application of the microbial manure in terms of preventing pear tree root rot containing bacillus mycoides Lzh Z7.The microbial manure that the experiment display present invention obtains can effectively prevent pear tree brown spot, improve the quality and yield of pears, have good application potential.
Description
Technical field
The present invention relates to pear tree rhizosphere growth-promoting bacillus mycoides Lzh-Z7, also relate to the bacterium and are passed in prevention pear tree soil
Application in terms of disease, belongs to field of biological control.
Background technology
Pears brown spot, also known as pears leaf pinta, pears spot blight, pears white star, by fungi pears brown patch germ
(Mycosphaerella sentina (Fr) Schrorter) causes.Main harm blade aggravates outburst, makes year by year in recent years
It at a large amount of early leaf falls, causes tree vigo(u)r weak, causes second bloom, influence to carry out annual output, this constitutes pears industry development very big
It threatens.The control method of pear tree brown spot has at present：Carry out clear garden work, reinforcement theatre manages, it is anti-to reinforce cultivation management, medicament
The serial of methods such as control, there are no efficient, less toxic and non-environmental-pollution biological methods for preventing pear tree brown spot.
Plant rhizosphere growth-promoting bacterium (plant growth promoting rhizobacteria, PGPR) is that existence is being planted
In object rhizosphere range, it can directly or indirectly promote plant growth and its absorption and utilization to mineral nutrition, and cause of disease can be inhibited
The beneficial mushroom of bacterium.In Agro-ecological System, the biological potentials of these bacteriums is made full use of to will be helpful to corps diseases
Prevention, promote plant growth.Researcher has screened the plant rhizosphere growth-promoting bacterium with notable biological activity, is used for micro- life
The exploitation of object fertilizer, can effectively inhibit plant disease, promote crop growth, improve quality of agricultural product, therefore by plant
Rhizosphere growth-promoting endophytic bacteria, which is added in organic fertilizer, is prepared into microbial manure, is with a wide range of applications.
Invention content
The purpose of the present invention is to provide one plant of pear tree rhizosphere growth-promoting bacillus mycoides Lzh-Z7 and its application, the present invention
It can safe and efficient control pear tree brown spot.
In the present invention, bacillus mycoides (Bacillus mycoides) Lzh-Z7 is preserved in Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, deposit number is CGMCC No.13491, and the deposit date is on December 23rd, 2016.
Preservation organization address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Bacterial strain Lzh-Z7 is identified by the methods of morphologic observation, dyeing, 16srRNA gene sequencings, as a result
For：
Bacterial strain Lzh-Z7 is gram-positive bacteria, is milky circular colonies on LB solid plates, opaque, bacterium colony is flat
Flat, edge is not rounded, surface wettability and easy picking.Through 16srRNA gene sequencings and tetraploid rice, with GenBank
In certain bacillus mycoides bacterial strain (GenBank accession number JX144726.1) have 99% homology, therefore be accredited as gill fungus shape gemma
Bacillus is named as bacillus mycoides Bacillus mycoides.
The invention also includes obtaining zymotic fluid by bacillus mycoides Lzh-Z7 fermentation, and contain bacillus mycoides
The microbial bacterial agent of Lzh-Z7 or bacillus mycoides Lzh-Z7 zymotic fluids.
The invention also includes the zymotic fluids of bacillus mycoides Lzh-Z7, bacillus mycoides Lzh-Z7, and contain gill fungus
The microbial bacterial agent of shape bacillus Lzh-Z7 or bacillus mycoides Lzh-Z7 zymotic fluids is in terms of preventing pear tree root rot
Application.
The invention also includes the microbial manures with prevention pear tree root rot, and microbial manure includes bacillus mycoides
In Lzh-Z7, the zymotic fluid of bacillus mycoides Lzh-Z7 or microbial bacterial agent containing bacillus mycoides Lzh-Z7 extremely
Few one kind.
Further, the invention also includes bacillus mycoides Lzh-Z7, bacillus mycoides Lzh-Z7 zymotic fluids or
Application of the microbial bacterial agent in prevention pear tree root rot or in terms of preparing microbial manure.
Further, the preparation method of bacillus mycoides Lzh-Z7 zymotic fluids, is as follows：
The seed liquor of bacillus mycoides Lzh-Z7 is inoculated in the beef extract-peptone fluid nutrient medium that pH is 7.0,
Inoculum concentration be 1% (v/v), under the conditions of 28 DEG C, 200rpm ferment 48h to get.
Further, the preparation method of the microbial bacterial agent containing bacillus mycoides Lzh-Z7, is as follows：
The zymotic fluid of bacillus mycoides Lzh-Z7 is added to the wheat bran of sterilizing according to the inoculum concentration of mass fraction 5%
In, 30 DEG C are cultivated 3 days, are air-dried to obtain the final product.
In the present invention, the preparation method of microbial manure is as follows：
(1) preparation of the microbial bacterial agent containing Acinetobacter junii Lzh-X15
The seed liquor of bacillus mycoides Lzh-Z7 is inoculated in the beef extract-peptone fluid nutrient medium that pH is 7.0,
Inoculum concentration is 1% (v/v), and ferment 48h under the conditions of 28 DEG C, 200rpm, then by zymotic fluid according to the inoculation of mass fraction 5%
Amount is added in the wheat bran of sterilizing, and 30 DEG C are cultivated 3 days, are air-dried to obtain the final product；
(2) it ferments
In mass ratio the 1 of microbial bacterial agent and organic fertilizer that step (1) is obtained:The ratio of 8-15 mixes, and after mixing, adjusts
Moisture content carries out solid fermentation to 50-60%, and fermentation temperature is no more than 50 DEG C, daily turning 2 times in fermentation process, when gill fungus shape
The content of bacillus Lzh-Z7 reaches 1 × 108To get the microorganism fertilizer of the Lzh-Z7 containing bacillus mycoides when cfu/g or more
Material.
Preferably, the volume mass of zymotic fluid and organic fertilizer ratio presses 1:10 ratio.
Further, the organic fertilizer is pig manure.
Compared with the prior art, the present invention has the following advantages：
The bacillus mycoides Lzh-Z7 that the present invention screens has extraordinary effect in terms of preventing pear tree brown spot,
And it can quickly prepare microbial manure using the zymotic fluid of bacillus mycoides Lzh-Z7 of the present invention.Potted plant experiment and big
Field experiment display, can effectively prevent pear tree brown spot using bacillus mycoides Lzh-Z7 microbial manures, improve the quality of pears
And yield.The present invention has the effect of efficient, nontoxic and non-environmental-pollution.
Description of the drawings
Fig. 1 is antagonistic effects of the bacillus mycoides Lzh-Z7 to pear tree brown patch germ, wherein Figure 1A is gill fungus shape gemma bar
Bacterium Lzh-Z7 is tested with pear tree brown patch germ opposite culture, and Figure 1B is control pear tree brown patch germ growing state.
Fig. 2 is that bacillus mycoides Lzh-Z7 microbial organic fertilizers try the potting of pear tree seedling rhizosphere growth-promoting effect
It tests.Wherein control is that brown spot is fallen ill soil, and processing 1 is fallen ill the Swine manure of soil+be not added with plant growth-promoting rhizobacteria for brown spot,
Processing 2 is brown spot morbidity soil+bacillus mycoides Lzh-Z7 microbial organic fertilizers.
Specific implementation mode
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art answer
It should be appreciated that can be repaiied without departing from the spirit and scope of the invention to the details and form of technical solution of the present invention
Change or replace, but these modifications and replacement are each fallen in protection scope of the present invention.
The screening and identification of 1 bacillus mycoides Lzh-Z7 of embodiment
Bacterial strain Lzh-Z7, which is infected from Dezhou City Shandong Province pear tree orchard in brown spot pear tree Rhizosphere Soil, to be separated to, specifically
Method is：
Plant growth-promoting rhizobacteria is screened from plant rhizosphere soil using tablet face-off method.Rhizosphere is taken from the root for the plant that catches an illness
Soil is sent as early as possible under cryogenic to laboratory treatment.Rhizosphere Soil is put into gradient dilution in sterile water, it is solid to be then applied to LB
On body culture medium, picking single bacterium colony after 30 DEG C of cultures for 24 hours.Gained single bacterium colony is inoculated on phytopathogen, sees whether generate
Antibacterial band, if there is antibionts activity.It is separated to bacterial strain Lzh-Z7 of the present invention by a large amount of screening test.
Bacillus mycoides Lzh-Z7 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, protects
It is CGMCC No.13491 to hide number, and the deposit date is on December 23rd, 2016.Preservation organization address：The Chaoyang District, Beijing City North Star
The institute 3 of West Road 1.Accession number of the 16S rRNA genes of the strain in GenBank is KY049896.
Bacterial strain Lzh-Z7 is identified by the methods of morphologic observation, dyeing, 16srRNA gene sequencings, as a result
For：
Bacterial strain Lzh-Z7 is gram-positive bacteria, is milky circular colonies on LB solid plates, opaque, bacterium colony is flat
Flat, edge is not rounded, surface wettability and easy picking.Through 16srRNA gene sequencings and tetraploid rice, with GenBank
In certain bacillus mycoides bacterial strain (GenBank accession number JX144726.1) have 99% homology, therefore be accredited as gill fungus shape gemma
Bacillus is named as bacillus mycoides Bacillus mycoides.
The antagonism of 2 bacillus mycoides Lzh-Z7 of embodiment
Using the antagonism of tablet face-off method detection bacillus mycoides Lzh-Z7.By pear tree Pathogenic Bacteria Causing Brown Blotch Disease
Mycosphaerella sentina (Fr) Schrorter is inoculated on PDA solid mediums (the potato 200g of peeling, sugarcane
Sugared 20g, agar 20g, distilled water 1000mL, natural pH), 30 DEG C culture 48hr after it is spare.Bacillus mycoides Lzh-Z7 is connect
Kind arrives Gao Shi solid mediums (soluble starch (20g), KNO3(1g), K2HPO4(0.5g), MgSO4·7H2O (0.5g), NaCl
(0.5g), FeSO4·7H2O (0.01g), agar 20g, distilled water 1000mL, pH=7.4-7.6) it is upper 30 DEG C culture 48hr be followed by
Kind arrives pear tree Pathogenic Bacteria Causing Brown Blotch Disease both sides, and 30 DEG C are continued to cultivate 48-72hr, see whether to generate antibacterial band.As a result such as Fig. 1 institutes
Show, the results showed that bacillus mycoides Lzh-Z7 of the present invention has good antagonism.
3 bacillus mycoides Lzh-Z7 microbial manures of embodiment and preparation method thereof
Preparation method is as follows：
(1) preparation of microbial bacterial agent
The seed liquor of bacillus mycoides Lzh-Z7 is inoculated in the beef extract-peptone fluid nutrient medium that pH is 7.0,
Inoculum concentration is 1% (v/v), and the 48h that ferments under the conditions of 28 DEG C, 200rpm is to get bacillus mycoides Lzh-Z7 zymotic fluids；It will hair
Zymotic fluid is added to according to the inoculum concentration of mass fraction 5% in the wheat bran of sterilizing, and 30 DEG C are cultivated 3 days, and it is gill fungus shape gemma bar to air-dry
Bacterium Lzh-Z7 microbial bacterial agents.
(2) preparation of microbial manure
The mass ratio of microbial bacterial agent and pig manure that step (1) is obtained presses 1:10 ratio mixes, and after mixing, adjusts
Moisture content carries out solid fermentation to 50-60%, and fermentation temperature is no more than 50 DEG C, daily turning 2 times in fermentation process, when gill fungus shape
The content of bacillus Lzh-Z7 reaches 1 × 108To get the microorganism fertilizer of the Lzh-Z7 containing bacillus mycoides when cfu/g or more
Material.
4 pot experiment of embodiment
Experiment microbial inoculum：The microbial manure of bacillus mycoides Lzh-Z7
Test site：Shandong Province Tai'an Daiyue District Shandong Fruit-tree Inst. day Pinghu proving ground
Experimental cultivar：Pear, stock are birch-leaf pear.
Control：Brown spot morbidity soil
Processing 1：The Swine manure of brown spot morbidity soil+do not add plant growth-promoting rhizobacteria
Processing 2：Microbial manure prepared by brown spot morbidity soil+embodiment of the present invention 3
In pot experiment, organic fertilizer is uniformly admixed with 3% amount of application (W/W) in the serious soil of brown spot morbidity.Three
After it, the consistent pear tree seedling of growing way is moved into basin alms bowl, basin alms bowl specification is 30cm × 40cm, and each processing 20 repeats 3
It is secondary.It cultivates in the greenhouse；After two months, plant disease index, incidence are investigated and recorded, indices, statistics biology are measured
Control effect, results from pot experiment test are as shown in Figure 2 and Table 1.
1 control effects of the bacillus mycoides Lzh-Z7 to pear tree brown spot of table and the facilitation to root growth
As shown in table 1：It is 61.03% to brown spot incidence after impinging upon the field planting of pears seedling；It is added in processing 1 and is not added with rhizosphere
The Swine manure of Promoting bacteria has equally infected brown spot, incidence 46.28%, but has been added to bacillus mycoides
After Lzh-Z7 microbial organic fertilizers (processing 2), incidence is substantially reduced, and incidence 18.37% illustrates bacillus mycoides
Lzh-Z7 microbial organic fertilizers have better biocontrol effect to pears brown spot；Simultaneously in processing 2, pear tree root biomass is notable
Increase, incrementss 147.21%, bacillus mycoides Lzh-Z7 microbial manures significantly improve root activity, promote root system
Growth.
6 field experiment of embodiment
Test site：Jinan City, Shandong Province Licheng District Middle Yellow River business office's pears base (Huashan town post-tensioning village).
Experimental cultivar：Golden pear, stock are birch-leaf pear, investigate its discovery and infect brown spot.
Test process：60 plants of brown spot disease plants are randomly choosed, experiment sets 4 processing：
Control：Not organic fertilizer
Processing 1：Using the Swine manure without plant growth-promoting rhizobacteria
Processing 2：The microbial manure prepared using embodiment 4
15 plants of consistent pear trees of each processing selection growing way.In November, 2015 applies each test group fertilizer, and use is radial
Fertilising, every plant of fertilising 5.0kg dig 3 bar ditch of depth 35cm, wide 25cm, after clay fertilizer mixing, bury soil, watering, other measures are the same as routine
Management, continuous processing 2 years.In the fructescence on the 27th of August in 2017, it is consistent, uniform in size, inorganic to randomly select maturity
Tool damages and 30 fruits of no disease and pests harm, measures fruit quality and index of correlation, and all indexs take the flat of 3 measurement results
Mean value.Plant disease index is investigated and recorded, control effect is calculated.
Test result is as shown in table 2, after continuous 2 years application bacillus mycoides Lzh-Z7 microbial manures, average fruit
Weight 334.52g, hardness of fruit 5.61kg/cm2, total Soluble Sugar 8.23%, organic acid content 0.19% and compares and handles one
It compares, mean fruit weight increases, and the hardness of fruit reduces, and total Soluble Sugar increases, and organic acid content reduces, it can be seen that, it applies
Fruit quality can be improved with bacillus mycoides Lzh-Z7 microbial manures.Microbial manure is remarkably improved fruit production simultaneously
Amount reduces incidence.
Shadow of the 2 bacillus mycoides Lzh-Z7 microbial manures of table to golden pear fruit quality, yield and its situation of catching an illness
It rings
Claims (10)
1. one plant of pear tree rhizosphere growth-promoting bacillus mycoides Lzh-Z7, which is characterized in that the bacillus mycoides Lzh-Z7 is protected
It is hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.13491, preservation
Date is on December 23rd, 2016, and preservation organization address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
2. a kind of zymotic fluid, which is characterized in that the zymotic fluid is obtained by bacillus mycoides Lzh-Z7 fermentations.
3. a kind of microbial bacterial agent, which is characterized in that the microbial bacterial agent includes bacillus mycoides Lzh-Z7 or gill fungus
The zymotic fluid of shape bacillus Lzh-Z7.
4. applications of the bacillus mycoides Lzh-Z7 as described in claim 1 in terms of preventing pear tree brown spot.
5. application of the zymotic fluid as claimed in claim 2 in terms of preventing pear tree brown spot.
6. application of the microbial bacterial agent as claimed in claim 3 in terms of preventing pear tree brown spot.
7. the preparation method of zymotic fluid as claimed in claim 2, which is characterized in that be as follows：
The seed liquor of bacillus mycoides Lzh-Z7 is inoculated in the beef extract-peptone fluid nutrient medium that pH is 7.0, inoculation
Amount be 1% (v/v), under the conditions of 28 DEG C, 200rpm ferment 48h to get.
8. a kind of preparation method of bacillus mycoides Lzh-Z7 microbial bacterial agents, which is characterized in that be as follows：
The zymotic fluid of bacillus mycoides Lzh-Z7 is added to according to the inoculum concentration of mass fraction 5% in the wheat bran of sterilizing, 30
DEG C culture 3 days, air-dry to obtain the final product.
9. a kind of microbial manure with prevention pear tree brown spot, which is characterized in that microbial manure includes gill fungus shape gemma bar
In bacterium Lzh-Z7, bacillus mycoides Lzh-Z7 zymotic fluids or microbial bacterial agent containing bacillus mycoides Lzh-Z7 extremely
Few one kind.
10. a kind of method preparing microbial manure as claimed in claim 9, which is characterized in that be as follows：
(1) preparation of the microbial bacterial agent containing Acinetobacter junii Lzh-X15
The seed liquor of bacillus mycoides Lzh-Z7 is inoculated in the beef extract-peptone fluid nutrient medium that pH is 7.0, inoculation
Amount is 1% (v/v), and ferment 48h under the conditions of 28 DEG C, 200rpm, then adds zymotic fluid according to the inoculum concentration of mass fraction 5%
Enter into the wheat bran of sterilizing, 30 DEG C are cultivated 3 days, are air-dried to obtain the final product；
(2) it ferments
In mass ratio the 1 of microbial bacterial agent and organic fertilizer that step (1) is obtained:The ratio of 8-15 mixes, and after mixing, adjusts water
Divide to 50-60%, carry out solid fermentation, fermentation temperature is no more than 50 DEG C, daily turning 2 times in fermentation process, when gill fungus shape gemma
The content of bacillus Lzh-Z7 reaches 1 × 108To get the microbial manure of the Lzh-Z7 containing bacillus mycoides when cfu/g or more；It is excellent
Choosing, the mass ratio of microbial bacterial agent and organic fertilizer is 1:10；It is furthermore preferred that the organic fertilizer is pig manure.
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Cited By (5)
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| CN110447660A (en) * | 2019-08-08 | 2019-11-15 | 甘肃农业大学 | A kind of microbial bacteria inhibitor and preparation method thereof |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
| CN115786192A (en) * | 2022-11-15 | 2023-03-14 | 广西民族大学 | Bacillus parasuis and application thereof |
| CN115851502A (en) * | 2022-09-28 | 2023-03-28 | 云南大学 | Bacillusmycoides, microbial inoculum, liquid-state biological organic fertilizer and application of bacillus mycoides, microbial inoculum and liquid-state biological organic fertilizer |
| CN117384807A (en) * | 2023-12-13 | 2024-01-12 | 中国农业大学 | Bacillus mycoides HDMM 2 and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110447660A (en) * | 2019-08-08 | 2019-11-15 | 甘肃农业大学 | A kind of microbial bacteria inhibitor and preparation method thereof |
| CN113897306A (en) * | 2021-09-13 | 2022-01-07 | 广西民族大学 | Compound microbial agent and application thereof |
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| CN115851502A (en) * | 2022-09-28 | 2023-03-28 | 云南大学 | Bacillusmycoides, microbial inoculum, liquid-state biological organic fertilizer and application of bacillus mycoides, microbial inoculum and liquid-state biological organic fertilizer |
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| CN115786192B (en) * | 2022-11-15 | 2023-05-12 | 广西民族大学 | Bacillus paramycoides and application thereof |
| CN117384807A (en) * | 2023-12-13 | 2024-01-12 | 中国农业大学 | Bacillus mycoides HDMM 2 and application thereof |
| CN117384807B (en) * | 2023-12-13 | 2024-03-12 | 中国农业大学 | Bacillus mycoides HDMM 2 and application thereof |
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