CN106701623A - Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus - Google Patents
Bacillus atrophaeus antagonistic to lycium chinensis root rot and application of bacillus atrophaeus Download PDFInfo
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Abstract
The invention discloses bacillus atrophaeus antagonistic to lycium chinensis root rot with the classification name of bacillus atrophaeus GQJK17, preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee of Culture Collection for Microorganisms) on November 10, 2016, with the strain preservation number of CGMCC NO.1 3254. The bacillus atrophaeus GQJK17 obtained through separation has a higher antagonistic effect on the fructus lycii root rot, a new biocontrol bacterium is provided for biocontrol of the lycium chinensis root rot, and the type of biocontrol preparations is increased.
Description
Technical field
Atrophy bacillus and its application the present invention relates to one plant of antagonism matrimony vine root rot, belong to biological technical field.
Background technology
Matrimony vine is a kind of rare Chinese medicine, and Chinese wolfberry fruit has nutrition and health care and medical value higher, with fine jade treasure ganoderma lucidum, length
White recluse's ginseng, Donga donkey-hide gelatin is simultaneously referred to as " Chinese medicine four is precious ".With expanding economy and the progress of society, people also increasingly recognize
To the value of matrimony vine, people are promoted to carry out extensive product development to matrimony vine, and achieve important achievement.Lycium barbarum is in
State's cultivated area is maximum, and as matrimony vine cultivated area constantly expands, old producing region is gone up year after year with the new producing region matrimony vine root rot incidence of disease
Rise, increasingly seriously, badly influenced local farmers income, have certain destruction to whole industrial chain.Chinese holly
Qi root rot is a kind of system infections disease, and diseased plant root and root neck occur to rot, and cane vascular bundle becomes brown.Disease portion when moist
White or pink mould layer (pathogen) is grown once in a while.Because root-rot, blade diminish, lack gloss, branch is downgraded, and fruit is thin
It is small；Or blade flavescence is wilted, plant is gradually withered when serious.Current people's preventing and treating matrimony vine root rot is mainly sprays insecticide, and matches somebody with somebody
Side's fertilising, the mode of increasing application phosphorus potassium fertilizer.But these methods waste time and energy, it is difficult to from root effective controlling disease generation, and
And it is big for environment pollution, also there is certain injury to health, and biological control method has selectivity and green ring because of it
The advantage of guarantor, is increasingly paid close attention to by people.There is bacillus for cotton wilt, potato sore using comparative maturity
The Biocontrol Effect of the plurality of plant diseases such as the red carninomatosis of scall, apple, root rotof flax, but without matrimony vine root rot biological control
The report of aspect, and the microorganism resource for finding antagonism matrimony vine root rot is crucial.
Atrophy bacillus (Bacillus atrophaeus) is a class mutation of bacillus subtilis, in agricultural, work
Industry, scientific research, medical treatment and health field etc. are all widely used.In countries and regions such as the U.S., Japan, European Union often by the bacterial strain
Bacterial strain and Evaluation of Germicidal Efficacy experiment indicator bacteria are detected as quality control standard.But the bacterium is applied to controlling plant diseases aspect
Report it is less, involved research mainly has：Preventing and treating balsam pear gray mold (CN105670964A), preventing and treating colorado potato bug larva
(CN104164378A) cotton boll blight (CN102747005A), preventing and treating tomato seedling damping-off, capsicum early blight, cotton standing dead, are prevented and treated
Sick (CN104531545A) etc., but do not have the report of the atrophy bacillus of preventing and treating matrimony vine root rot also at present.And, biological and ecological methods to prevent plant disease, pests, and erosion is micro-
Often exist between microbiologic population in biological and soil environment and interact, the introducing of external source Antagonistic Fungi can cause crop
The composition of rhizosphere soil microorganism group changes, and the field planting of different plant rhizosphere bacteriums of the same race and function have larger difference
Property；And, can Biocontrol microorganism survive in soil environment and fast-growth is also the key for realizing preventive and therapeutic effect.Cause
This, the biocontrol bacterial strain that screening can have preventive and therapeutic effect to matrimony vine root rot has highly important actual application value.
The content of the invention
For above-mentioned prior art, it is an object of the invention to provide one plant of antagonism matrimony vine root rot atrophy bacillus and
Its application.
To achieve the above object, the present invention uses following technical proposals：
According to the first aspect of the invention, there is provided one plant of atrophy bacillus of antagonism matrimony vine root rot, its Classification And Nomenclature
It is atrophy bacillus (Bacillus atrophaeus) GQJK17, is preserved in China Microbiological on November 10th, 2016
(abbreviation CGMCC, address is culture presevation administration committee common micro-organisms center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, Institute of Microorganism, Academia Sinica), culture presevation number is CGMCC NO.13254.
The bacterial strain is isolated from matrimony vine rhizosphere soil, and the bacterium colony and thalline of the bacterial strain are characterized as：On LB flat boards
Culture 24h after bacterium colony be in subcircular, milky, bacterium colony flat, the small dentation in edge, dry tack free, colony diameter is 2.62mm.
Thalline is in elongated rod shape, produces gemma, Gram-positive.
The physiological and biochemical property of the bacterial strain is：Oxidizing ferment experiment is negative, and catalase experiment is positive, and citrate is using experiment
The positive, nitrate is negative using experiment, and Starch Hydrolysis experiment is positive, and M-R experiments are negative, and V-P experiments are positive, glucose fermentation reality
The positive is tested, sucrose fermenting experiment is positive, and maltose fermenting experiment is negative, and arabinose fermenting experiment is positive, wood-sugar fermentation experiment
Feminine gender, cellobiose fermenting experiment is negative, and lactose fermentation experiment is negative, and sorbitol fermentation experiment is positive, mannose ferment experiment
It is positive.
According to the second aspect of the invention, there is provided a kind of biological prevention and control agent, its active component is above-mentioned atrophy bacillus
The tunning of (Bacillus atrophaeus) GQJK17.
Preferably, the biological prevention and control agent is liquid microbial inoculum.
Further, in the biological prevention and control agent atrophy bacillus (Bacillus atrophaeus) GQJK17 content
More than or equal to 2 × 108cfu/mL。
The present invention also provides the preparation method of above-mentioned biological prevention and control agent, comprises the following steps：The above-mentioned atrophy gemma bar of fermentation
Bacterium (Bacillus atrophaeus) GQJK17, obtains tunning.
In above-mentioned preparation method, the culture medium that fermentation is used is liquid bean sprouts medium, and its raw material composition is：Moyashi
100g, sucrose 50g, water 1000mL, pH nature；
The preparation method of the liquid bean sprouts medium is：Moyashi boiling water boiling 30min, with four layers of filtered through gauze,
Add sucrose constant volume to 1000mL.
In above-mentioned preparation method, the condition of fermentation is：35-40 DEG C, constant-temperature table culture 20-30h；Preferably, it is 37 DEG C, permanent
Warm shaking table culture 24h.
According to the third aspect of the invention we, there is provided above-mentioned atrophy bacillus (Bacillus atrophaeus) GQJK17
And/or application of the biological prevention and control agent in matrimony vine root rot is prevented and treated.
In above-mentioned application, the preventing and treating matrimony vine root rot is embodied in the breaking-out for suppressing matrimony vine root rot, reduces the incidence of disease.
According to the fourth aspect of the invention, there is provided above-mentioned atrophy bacillus (Bacillus atrophaeus) GQJK17
And/or application of the biological prevention and control agent in matrimony vine volume increase is promoted.
The present invention also provides one kind and can prevent and treat matrimony vine root rot, while the product of volume increase, its active component is above-mentioned withering
Contracting bacillus (Bacillus atrophaeus) GQJK17 or above-mentioned biological prevention and control agents.
Beneficial effects of the present invention：
(1) isolated atrophy bacillus (Bacillus atrophaeus) GQJK17 of the present invention, the bacterial strain is to Chinese holly
Qi root rot has stronger antagonism, for the biological control of matrimony vine root rot provides new biocontrol microorganisms, increased biological and ecological methods to prevent plant disease, pests, and erosion system
The species of agent.
(2) research shows, under field condition, using atrophy bacillus (Bacillus of the invention
Atrophaeus after) the matrimony vine tree in the bacteria suspension of the GQJK17 plot serious to the morbidity of matrimony vine root rot carries out root irrigation, energy
Substantially suppress the breaking-out of matrimony vine root rot, reduce the incidence of disease；Atrophy bacillus (Bacillus atrophaeus) of the invention
GQJK17 can also improve the upgrowth situation of plant, improve the yield of matrimony vine, and matrimony vine tree is averagely increased production more than 484%.More than
Result of study shows that there is the bacillus of antagonism matrimony vine root rot of the invention certain preventing and treating to imitate to lycium barbarum root rot
Really.
(3) root rot of different plants, its pathogen is different, and pathogenesis can also have differences.To the root of other plant
Maize ear rot has the bacillus of prevention effect, can not directly deduce that it has prevention effect to matrimony vine root rot.We were testing
Find there is the bacterial strain of antagonistic effect to phytopathogen in flat board face-off experiment, but do not ensure that it in crop field reality in journey
Effect in testing.The result of field experiment also demonstrates that, possesses preferable flat board and stands facing each other the bacterial strain of experimental result, in field experiment
In, obvious inhibition may not also be possessed to matrimony vine Pathogens Causing Root Rot Disease.Therefore, the root rot to other plant has preventing and treating to imitate
For the bacillus of fruit, due to the severe living environment in Ningxia, China, can bacterial strain survive and fast-growth turns into key
Problem, therefore may not have prevention effect to matrimony vine root rot.
Brief description of the drawings
Fig. 1：The colonial morphology figure of atrophy bacillus (Bacillus atrophaeus) GQJK17；
Fig. 2：The phyletic evolution tree graph of atrophy bacillus (Bacillus atrophaeus) GQJK17.
Specific embodiment
Involved instrument, reagent, material etc. in following embodiments, unless otherwise noted, are existing in the prior art
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Involved experimental technique in following embodiments, inspection
Survey method etc., unless otherwise noted, is existing normal experiment method in the prior art, detection method etc..
Embodiment 1：The separation of bacterial strain, screening
(1) separation of strain
Matrimony vine tree root border pedotheque 5 healthy, dying, in heaven is gathered from Zhongning County Shi Laba villages of Ningxia Province.Weigh
10.0 grams of matrimony vine Rhizosphere Soils, add the ratio of 90mL sterilized waters to be put into triangle of the preprepared equipped with bead in every 10 grams of soil
In bottle, 180rpm concussions 20-30min on 37 DEG C of constant-temperature tables is placed in.Gradient dilution method, 10 are diluted to by soil supension-2、10-3、10-4、10-5、10-6Etc. different gradients.The soil liquid 0.1mL for drawing appropriate dilution is placed in LB plating mediums, is coated with
Uniformly (this experiment chooses 10-4、10-5、10-6Three gradients are coated).37 DEG C of constant incubator culture 24h, picking single bacterium
Fall.The bacterial strain of acquisition is purified with the method for three rides, until obtaining single bacterium colony, and bacterial strain is numbered, protected
It is stored in 4 DEG C of refrigerators standby.
(2) screening of Antagonistic Fungi
Primary election is carried out to Antagonistic Fungi using flat board face-off method.The bacterial strain activation that will be preserved, it is standby.To be stored on inclined-plane
Matrimony vine Pathogens Causing Root Rot Disease is activated in PDA plate using scribble method, 28 DEG C of cultures to covering with flat board, with the blue electron gun head of sterilizing or
Above-mentioned pathogen bacterium block, the middle part of PDA plate is transferred to tweezers under card punch vertical cutting, is placed in 28 DEG C of incubator cultures
One day.The Antagonistic Fungi activated with toothpick picking is inoculated at pathogen edge 2cm, is placed in 28 DEG C of incubators and is continued to cultivate 2-
5 days, the appearance of antibacterial band is seen whether, measure the width of antibacterial band.Bacteriostatic diameter is measured simultaneously, calculates bacteriostasis rate.
Bacteriostasis rate (%)=(colony diameter D- bacteriostatic diameters d)/colony diameter D × 100%.
2. result of the test：
The selection best bacterial strain of one plant of fungistatic effect, strain number is GQJK17, and its flat board dual test the results are shown in Table
1。
Table 1：Bacteriostasis rate measurement result
As can be seen from Table 1：Bacterial strain GQJK17 has preferable bacteriostasis to the pathogen of matrimony vine root rot.
2. the form of bacterial strain GQJK17 and physiological and biochemical property are determined
(1) colony characteristicses and thalli morphology
The bacterium colony and thalline of the bacterial strain are characterized as：Bacterium colony is in subcircular, milky, bacterium colony after cultivating 24h on LB flat boards
Flat, the small dentation in edge, dry tack free, colony diameter is 2.62mm.Thalline is in elongated rod shape, produces gemma, Gram-positive.
(2) physiological and biochemical property
The physiological and biochemical property of the bacterial strain is：Oxidizing ferment experiment is negative, and catalase experiment is positive, and citrate is using experiment
The positive, nitrate is negative using experiment, and Starch Hydrolysis experiment is positive, and M-R experiments are negative, and V-P experiments are positive, glucose fermentation reality
The positive is tested, sucrose fermenting experiment is positive, and maltose fermenting experiment is negative, and arabinose fermenting experiment is positive, wood-sugar fermentation experiment
Feminine gender, cellobiose fermenting experiment is negative, and lactose fermentation experiment is negative, and sorbitol fermentation experiment is positive, mannose ferment experiment
It is positive.
3. the 16S rDNA of bacterial strain GQJK17 are determined and analysis
The 16S rDNA gene orders of bacterial strain GQJK17, as shown in SEQ ID NO.1 in nucleotides sequence list.To be surveyed
Sequence alignment in 16S rDNA sequences and GenBank databases, as a result shows：GQJK17 and Bacillus atrophaeus
(JCM 9070) is in together in a minimum branch, its 16S rRNA gene order and Bacillus atrophaeus (NBRC
15539) strain sequence similarity is 99%, with reference to above-mentioned physiological and biochemical property result, identifies that GQJK17 is atrophy gemma bar
Bacterium (Bacillus atrophaeus).
Bacterial strain GQJK17, Classification And Nomenclature be atrophy bacillus (Bacillus atrophaeus) GQJK17, in
On November 10th, 2016 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (abbreviation CGMCC, address
For：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), culture presevation number is CGMCC
NO.13254。
Embodiment 2：The preparation of bacterial strain GQJK17 biological prevention and control agents
Specific preparation method is as follows：
Atrophy bacillus (Bacillus atrophaeus) GQJK17 for the antagonism matrimony vine root rot that inclined-plane is preserved turns
It is connected in the test tube equipped with 5mL bean sprout juice fluid nutrient mediums, is put in 37 DEG C, cultivates 12h in 180rpm constant-temperature tables.Then press
1% inoculum concentration is inoculated into the liquid bean sprouts medium of 100mL/250mL, is put in 37 DEG C, is trained in 180rpm constant-temperature tables
24h is supported, it is 2 × 10 to be made concentration8The bacteria suspension of cfu/mL.
Liquid bean sprouts medium, its raw material constitutes and is：Moyashi 100g, sucrose 50g, water 1000mL, pH nature；
Preparation method is：Moyashi boiling water boiling 30min, with four layers of filtered through gauze, adds sucrose constant volume to 1000mL.
Embodiment 3：Field test
1. test method：
The serious plot of selection matrimony vine root rot morbidity, designs two treatment, respectively processes 1：GQJK17 bacterium solutions (are implemented
It is prepared by example 2), every matrimony vine tree consumption is 25mL；Treatment 2：Compared with the clear water of equivalent.
Each treatment sets 3 repetitions, and each repeats to select 20 matrimony vine trees.
Specific test method：Pouring root is carried out to matrimony vine tree after bacterium solution is diluted into 200 times with clear water.The clear water of control equivalent
Pouring root.
2. result of the test：
(1) prevention effects of the bacterial strain GQJK17 to matrimony vine root rot
Result is as shown in table 2.
Table 2：Matrimony vine tree morbidity statistics in field experiment
As can be seen from Table 2：
1. the incidence of disease：In field experiment, the incidence of disease of control is 90%, with atrophy bacillus (Bacillus
Atrophaeus) the matrimony vine tree incidence of disease of GQJK17 bacterium solutions treatment is 41.67%, compared with the control, the Chinese holly processed with GQJK17
The incidence of disease of Qi Shu reduces 48.33 percentage points.
2. relative control effect：Atrophy bacillus (Bacillus atrophaeus) GQJK17 is relative to matrimony vine root rot
Preventive effect is up to 63%.
(2) effect of increasing production of the bacterial strain GQJK17 to matrimony vine tree
The results are shown in Table 3.
Table 3：Effect of increasing production of atrophy bacillus (Bacillus atrophaeus) GQJK17 to matrimony vine tree
As can be seen from Table 3：The matrimony vine tree average product of control treatment is 0.5kg/, uses atrophy bacillus
The yield of (Bacillus atrophaeus) GQJK17 bacterium solutions treatment is 2.92kg/, compared with the control, with GQJK17 treatment
Matrimony vine tree averagely volume increase 484%.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
It is any to be familiar with those skilled in the art in the technical scope of present disclosure, technology according to the present invention scheme and its invention
Design is subject to equivalent or change, should all be included within the scope of the present invention.
SEQUENCE LISTING
<110>Shandong Agricultural University
<120>The atrophy bacillus of one plant of antagonism matrimony vine root rot and its application
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1421
<212> DNA
<213>Bacterial strain GQJK17
<400> 1
cttcggcggc tggctcataa aggttacctc accgacttcg ggtgttacaa actctcgtgg 60
tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcatgc tgatccgcga 120
ttactagcga ttccagcttc acgcagtcga gttgcagact gcgatccgaa ctgagaacag 180
atttgtggga ttggcttaac ctcgcggtct cgctgccctt tgttctgtcc attgtagcac 240
gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc ttcctccggt 300
ttgtcaccgg cagtcacctt agagtgccca actgaatgct ggcaactaag atcaagggtt 360
gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca accatgcacc 420
acctgtcact ctgcccccga aggggaagcc ctatctctag gggtgtcaga ggatgtcaag 480
acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg cttgtgcggg 540
cccccgtcaa ttcctttgag tttcagtctt gcgaccgtac tccccaggcg gagtgcttaa 600
tgcgttagct gcagcactaa ggggcggaaa ccccctaaca cttagcactc atcgtttacg 660
gcgtggacta ccagggtatc taatcctgtt cgctccccac gctttcgctc ctcagcgtca 720
gttacagacc agagagtcgc cttcgccact ggtgttcctc cacatctcta cgcatttcac 780
cgctacacgt ggaattccac tctcctcttc tgcactcaag ttccccagtt tccaatgacc 840
ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc gagcccttta 900
cgcccaataa ttccggacaa cgcttgccac ctacgtatta ccgcggctgc tggcacgtag 960
ttagccgtgg ctttctggtt aggtaccgtc aaggtgccgc cctatttgaa cggcacttgt 1020
tcttccctaa caacagagct ttacgatccg aaaaccttca tcactcacgc ggcgttgctc 1080
cgtcagactt tcgtccattg cggaagattc cctactgctg cctcccgtag gagtctgggc 1140
cgtgtctcag tcccagtgtg gccgatcacc ctctcaggtc ggctacgcat cgttgccttg 1200
gtgagccatt acctcaccaa ctagctaatg cgccgcgggt ccatctgtaa gtggtagccg 1260
aagccacctt ttatgtttga accatgcggt tcaaacaagc atccggtatt agccccggtt 1320
tcccggagtt atcccagtct tacaggcagg ttacccacgt gttactcacc cgtccgccgc 1380
taacatcagg gagcaagctc ccatctgtcc gctcgactgc a 1421
Claims (10)
1. one plant of atrophy bacillus of antagonism matrimony vine root rot, its Classification And Nomenclature is atrophy bacillus (Bacillus
Atrophaeus) GQJK17, has been preserved in China Committee for Culture Collection of Microorganisms commonly micro- on November 10th, 2016
(abbreviation CGMCC, address is Bio-Centers：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute), culture presevation number is CGMCC NO.13254.
2. a kind of biological prevention and control agent, its active component is the atrophy bacillus (Bacillus described in claim 1
Atrophaeus) the tunning of GQJK17.
3. biological prevention and control agent as claimed in claim 2, it is characterised in that the biological prevention and control agent is liquid bacterial agent.
4. biological prevention and control agent as claimed in claim 3, it is characterised in that atrophy bacillus in the biological prevention and control agent
The content of (Bacillus atrophaeus) GQJK17 is more than or equal to 2 × 108cfu/mL。
5. the preparation method of the biological prevention and control agent described in any one of claim 2-4, it is characterised in that comprise the following steps：Fermentation
Atrophy bacillus (Bacillus atrophaeus) GQJK17 described in claim 1, obtains tunning.
6. preparation method as claimed in claim 5, it is characterised in that the culture medium that fermentation is used is liquid bean sprout juice culture
Base, its raw material constitutes and is：Moyashi 100g, sucrose 50g, water 1000mL, pH nature.
7. preparation method as claimed in claim 5, it is characterised in that the condition of fermentation is：35-40 DEG C, constant-temperature table culture
20-30h；Preferably, 37 DEG C, constant-temperature table culture 24h.
8. atrophy bacillus (Bacillus atrophaeus) GQJK17 described in claim 1 and/or claim 2 institute
Application of the biological prevention and control agent stated in matrimony vine root rot is prevented and treated.
9. atrophy bacillus (Bacillus atrophaeus) GQJK17 described in claim 1 and/or claim 2 institute
Application of the biological prevention and control agent stated in matrimony vine volume increase is promoted.
10. one kind can prevent and treat matrimony vine root rot, while the product of volume increase, its active component is the atrophy bud described in claim 1
Biological prevention and control agent described in spore bacillus (Bacillus atrophaeus) GQJK17 or claim 2.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810918A (en) * | 2019-01-26 | 2019-05-28 | 武威市林业科学研究院 | One plant of atrophy bacillus, bacteria agent and its application for having preventive effect to fructus lycii leaf blight |
| CN111662837A (en) * | 2019-03-07 | 2020-09-15 | 宁夏大学 | Bacillus atrophaeus and application thereof |
| CN112625958A (en) * | 2020-12-28 | 2021-04-09 | 中国林业科学研究院森林生态环境与保护研究所 | Bacillus atrophaeus, microbial inoculum and application thereof |
| CN113699075A (en) * | 2021-09-03 | 2021-11-26 | 山东农业大学 | Bacillus atrophaeus capable of producing protease and decomposing potassium and application thereof |
| CN114196565A (en) * | 2021-10-14 | 2022-03-18 | 河南农业大学 | Bacillus atrophaeus XZRKZ-23 and application thereof |
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| US20160270407A1 (en) * | 2013-11-08 | 2016-09-22 | Novozymes Bioag A/S | Compositions and methods for treating pests |
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| CN105695366A (en) * | 2016-04-05 | 2016-06-22 | 中国农业科学院植物保护研究所 | Bacillus strain and application thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109810918A (en) * | 2019-01-26 | 2019-05-28 | 武威市林业科学研究院 | One plant of atrophy bacillus, bacteria agent and its application for having preventive effect to fructus lycii leaf blight |
| CN111662837A (en) * | 2019-03-07 | 2020-09-15 | 宁夏大学 | Bacillus atrophaeus and application thereof |
| CN111662837B (en) * | 2019-03-07 | 2023-01-24 | 宁夏大学 | Bacillus atrophaeus and application thereof |
| CN112625958A (en) * | 2020-12-28 | 2021-04-09 | 中国林业科学研究院森林生态环境与保护研究所 | Bacillus atrophaeus, microbial inoculum and application thereof |
| CN113699075A (en) * | 2021-09-03 | 2021-11-26 | 山东农业大学 | Bacillus atrophaeus capable of producing protease and decomposing potassium and application thereof |
| CN114196565A (en) * | 2021-10-14 | 2022-03-18 | 河南农业大学 | Bacillus atrophaeus XZRKZ-23 and application thereof |
| CN114196565B (en) * | 2021-10-14 | 2024-03-01 | 河南农业大学 | Bacillus atrophaeus XZRKZ-23 and application thereof |
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|---|---|
| CN106701623B (en) | 2019-07-30 |
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