CN115433029A - Special biological fertilizer for preventing and treating sweet potato black spot diseases and insect pests and preparation method and application thereof - Google Patents
Special biological fertilizer for preventing and treating sweet potato black spot diseases and insect pests and preparation method and application thereof Download PDFInfo
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- CN115433029A CN115433029A CN202211229379.4A CN202211229379A CN115433029A CN 115433029 A CN115433029 A CN 115433029A CN 202211229379 A CN202211229379 A CN 202211229379A CN 115433029 A CN115433029 A CN 115433029A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
- C05D1/02—Manufacture from potassium chloride or sulfate or double or mixed salts thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/70—Controlling the treatment in response to process parameters
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a biological special fertilizer for preventing and treating sweet potato black spot diseases and insect pests, and a preparation method and application thereof, wherein the biological special fertilizer comprises the following components in parts by weight: 75-85 parts of organic fertilizer, 1-2 parts of bacillus amyloliquefaciens, 1-2 parts of paenibacillus polymyxa, 1-2 parts of bacillus thuringiensis, 1-2 parts of beauveria bassiana, 1-2 parts of metarhizium anisopliae and 10-15 parts of potassium sulfate; the organic fertilizer is prepared from the following raw materials: 60-80 parts of cattle and sheep manure, 15-30 parts of straw, 5-10 parts of PAL material and 1-3 parts of zymophyte. The biological special fertilizer for preventing and treating the black spot disease and the grub of the sweet potatoes can effectively reduce the use of pesticides and chemical fertilizers, relieve the non-point source pollution of the chemical fertilizers and the pesticides to soil, improve the soil structure, efficiently combine the organic fertilizers and beneficial microorganisms for use, improve the utilization rate of the sweet potatoes to high fertilizers and the capability of resisting diseases and insect pests, further improve the quality of the sweet potatoes and improve the yield.
Description
Technical Field
The invention relates to the field of crop pest control and microbial fertilizer, in particular to a biological special fertilizer for controlling sweet potato black spot disease and insect pest, and a preparation method and application thereof.
Background
The sweet potato is a crop used as grain, feed and industrial raw materials, and has a large planting area and a wide planting area in China. Because the sweet potato blocks contain rich edible fiber, soluble sugar, various amino acids, vitamins and mineral substances, the consumption proportion of the sweet potato is increased along with the improvement of health care consciousness of people in recent years, and the stem leaves of the sweet potato contain rich crude protein and can be used as high-quality animal feed. However, in the sweet potato planting process, soil improvement is not paid enough attention, and phenomena of long-term application of nitrogen fertilizer, unbalanced fertilization, pesticide abuse and the like are serious, so that not only is resource waste caused, but also the yield and the quality of the sweet potatoes are influenced, the physical properties of the soil are deteriorated, and the production benefit of the sweet potatoes is reduced. Especially in northern Anhui plain areas, soil is hardened, the air permeability is poor, the types and the quantity of microorganisms are insufficient due to the missing organic fertilizer for many years, the continuous deterioration of the soil environment also provides a favorable place for the occurrence of soil-borne diseases, especially the black spot and the root rot of sweet potatoes, the disease is spread quickly and causes large loss, at present, the prevention and the treatment of chemical agents are mainly used, and the long-term use of chemical agents can not only lead pathogenic bacteria to generate certain drug resistance; along with the work of burning-forbidden straws and pesticide reduction, a favorable space is provided for propagation of underground pests, wherein the mole cricket larvae and the scarab larvae have great harm to the quality and the grade of the sweet potato tubers, and the planting enthusiasm of farmers is gradually influenced. With the rapid development of biotechnology and the intensive research on soil physicochemical, the biological control and organic fertilizer investment are tried to be utilized to improve the growth environment of sweet potatoes, and the sweet potatoes are more and more accepted by people.
Chinese invention patent, application publication No. CN 102249796B inorganic compound fertilizer special for sweet potato with insect prevention effect and application thereof, discloses an organic fertilizer obtained by fermenting tobacco platycodon root or Chinese herb residue and livestock and poultry manure, and then taking the organic fertilizer and urea, calcium superphosphate and potassium sulfate compound fertilizer as base fertilizer for preventing and treating underground pests; chinese invention patent, application publication No. CN 113698250A biological prevention and control fertilizer for sweet potato diseases and insect pests, and preparation method and application thereof disclose a biological organic fertilizer for preventing and controlling diseases and insect pests, which adopts saccharomycetes, bacillus subtilis, bacillus cereus, aspergillus oryzae, trichoderma viride to ferment corn stalks and livestock and poultry manure; chinese invention patent, application publication No. CN 106397067A, an organic fertilizer for preventing and treating black spot disease of sweet potato and its preparation method, discloses a fertilizer prepared by using Chinese medicine residue and scorpion residue as raw materials, inoculating a compound microbial inoculum containing bacteria, fungi and actinomycetes, and fermenting to obtain the special fertilizer for preventing and treating sweet potato disease.
Aiming at the requirements of pest control of sweet potato black spot and grub in northern Anhui area, the special fertilizer technology for controlling sweet potato pest belongs to the traditional organic fertilizer fermentation method, livestock and poultry manure or pesticide residues and conventional microbial strains are mixed and fermented to formulate a biological organic fertilizer with control effect, and the targeted control of sweet potato black spot and grub can not be realized, and the pest control and the soil improvement can not be effectively unified.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a special biological fertilizer for preventing and treating sweet potato black spot pests, a preparation method and application thereof.
The invention is realized by the following technical scheme:
a biological special fertilizer for preventing and treating sweet potato black spot diseases and insect pests comprises the following components in parts by weight: 75-85 parts of organic fertilizer, 1-2 parts of bacillus amyloliquefaciens, 1-2 parts of paenibacillus polymyxa, 1-2 parts of bacillus thuringiensis, 1-2 parts of beauveria bassiana, 1-2 parts of metarhizium anisopliae and 10-15 parts of potassium sulfate; the organic fertilizer is prepared from the following raw materials: 60-80 parts of cattle and sheep manure, 15-30 parts of straw, 5-10 parts of PAL material and 1-3 parts of zymophyte.
In the biological special fertilizer for preventing and treating the sweet potato black spot and the grub, the selected bacillus amyloliquefaciens and the selected paenibacillus polymyxa have high propagation speed, preferentially occupy soil nutrient sites, improve the ecological environment of the rhizosphere of the sweet potato, and simultaneously have strong antagonistic action on a pathogen of the sweet potato black spot, namely the sweet potato rostratum Ellis et Halsted, have great influence on the sporulation type of the alternaria nigra and further inhibit the propagation of pathogenic bacteria. The selected bacillus thuringiensis can produce parasporal crystals (the main component is protein), and the parasporal crystals are damaged into small units of delta-endotoxin by insect alkaline intestinal juice, so that pests are killed due to septicemia. The selected beauveria bassiana and the spores emitted by the green muscardine fungi. When the spore contacts with pests, it germinates at proper temperature, and the growing hypha invades into the pests to produce large amount of hypha and secretion, so that the pests are ill and die after about 4-5 days, and the body surface is full of hypha and white powdery spore. The spores can continuously spread by wind, insects and the like to infect other pests.
The special biological fertilizer for preventing and treating sweet potato black spot and golden grub is prepared from the following strains in proportion by selecting a microbial fermentation inoculant: bai Jianhui streptomyces, saccharomyces cerevisiae, bacillus subtilis, bacillus thermophilus, trichoderma viride, in a ratio of 5. The compound microbial inoculum can be metabolized to generate various extracellular enzymes including protease, amylase, cellulase and the like, can decompose protein and cellulose in cattle and sheep manure and straws to be simple organic matters which can be directly absorbed and utilized by crops, can generate a large amount of heat in the fermentation process to effectively kill pathogenic bacteria and parasitic ova in the fertilizer, and can also continuously play roles in the aspects of preventing plant diseases and insect pests, improving soil structure, improving fertilizer utilization rate and the like.
PAL material, commonly named palygorskite, also called attapulgite, containing SiO for fermentation of the special biological fertilizer for preventing and treating sweet potato black spot and golden grub 2 、AlO 3 、FeO 3 、MgO、CaO、K 2 O、Na 2 O、P 2 O 5 The fertilizer contains various nutrient elements required by plants, has a structure of nano-scale porous nonmetallic minerals, has good adsorption performance, is an important carrier of bioactive substances, and provides a good carrier for various microbial bacteria in special fertilizers.
The preparation method of the biological special fertilizer for preventing and treating the black spot and the grub of the sweet potatoes comprises the following steps:
(1) Crushing corn straws or wheat straws into powder, uniformly mixing the crushed straws with cattle and sheep excrement to obtain a mixed material, adding a zymophyte agent into the mixed material, uniformly mixing, adjusting the water content of the mixed material to be 60-70%, performing composting, decomposing and fermenting, crushing by a crusher after fermentation is completed, and sieving to obtain an organic fertilizer;
(2) Uniformly mixing the organic fertilizer with bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus thuringiensis, beauveria bassiana, metarhizium anisopliae and potassium sulfate to obtain the special biological fertilizer for preventing and treating black spot disease and grubs of sweet potatoes
As a further improvement to the invention, the specific process of compost decomposition and fermentation in the step (1) is as follows: performing composting treatment for 7-10 days, performing first pile turning at a fermentation temperature of above 50 ℃, continuing to perform fermentation for 10-15 days after pile turning, performing second pile turning, continuing to perform fermentation for 10-15 days, transferring to an aging tank, aging for 20-30 days, and completing fermentation.
Compared with the prior art, the invention has the following advantages:
(1) The biological special fertilizer for preventing and treating the black spot disease and the grub of the sweet potatoes adopts beneficial microbial strains to prevent and treat the black spot disease and the grub of the sweet potatoes, has stronger pertinence, particularly has stronger preventing effect on the black spot disease of the sweet potatoes by bacillus amyloliquefaciens and paenibacillus polymyxa, has stronger infection lethal effect on grub larvae and imagoes by bacillus thuringiensis, beauveria bassiana and metarhizium anisopliae, can reduce the use of pesticides, and simultaneously can also have synergistic antibacterial and insect-preventing effects by the microbial strains used for fermenting organic fertilizers. The beneficial microorganisms can also accelerate the degradation and transformation of soil organic matters, improve the granular structure of soil and purify the rhizosphere environment of the sweet potatoes.
(2) The special biological fertilizer for preventing and treating the black spot disease and the grub of the sweet potatoes selects the PAL material as a biological carrier, has stronger adsorption function, can provide a living place for beneficial bacteria in the special fertilizer, and is beneficial to the propagation and growth of the beneficial bacteria.
(3) The biological special fertilizer for preventing and treating the black spot disease and the grub of the sweet potatoes can effectively reduce the use of pesticides and chemical fertilizers, relieves the non-point source pollution of the chemical fertilizers and the pesticides to soil, can improve the utilization rate of the fertilizer and the disease and insect pest resistance of the sweet potatoes by efficiently combining the organic fertilizer and beneficial microorganisms, further improves the quality of the sweet potatoes, improves the yield, is environment-friendly, is simple to use, and has great advantages in popularization and application in the aspect of sweet potato planting.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
1. Material
The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used in this example are commercially available products unless otherwise specified.
2. Method of producing a composite material
2.1 preparation of biological Special-purpose Fertilizer
The special biological fertilizer for preventing and treating the black spot and the grub of the sweet potatoes is prepared from the following raw materials in parts by weight as shown in table 1:
table 1: raw material composition of special fertilizer
| Group of | Example 1 | Example 2 | Example 3 | Example 4 |
| Organic fertilizer | 80 | 75 | 85 | 80 |
| Bacillus amyloliquefaciens | 1 | 2 | 1 | 2 |
| Paenibacillus polymyxa | 1 | 2 | 1 | 2 |
| Bacillus thuringiensis | 1 | 2 | 1 | 2 |
| Beauveria bassiana (balsamo) Vuillemin | 1 | 2 | 1 | 2 |
| Metarrhizium anisopliae | 1 | 2 | 1 | 2 |
| Potassium sulfate | 15 | 15 | 10 | 10 |
Wherein the compositions of the organic fertilizers of examples 1-4 are shown in Table 2:
table 2: organic fertilizer composition
| Group of | Example 1 | Example 2 | Example 3 | Example 4 |
| Cattle and sheep manure | 70 | 75 | 65 | 60 |
| Straw and stalk | 20 | 12 | 22 | 30 |
| PAL material | 8 | 10 | 10 | 7 |
| Fermentation inoculum | 2 | 3 | 3 | 3 |
The fermentation inoculum consists of the following components in proportion: the mass ratio of Bai Jianhui streptomyces, saccharomyces cerevisiae, bacillus subtilis, bacillus thermophilus and trichoderma viride is 5.
The preparation method of the biological special fertilizer for preventing and treating the black spot and the grub of the sweet potatoes comprises the following steps:
(1) Pulverizing corn stalk or wheat stalk into powder;
(2) Uniformly mixing the crushed straws with the cattle and sheep manure to obtain a mixed material, adding a microbial fermentation inoculant into the mixed material, uniformly mixing, adjusting the water content of the mixed material to be 60-70%, and performing compost maturity fermentation;
(3) Performing composting treatment for 10 days, performing first pile turning at a fermentation temperature of more than 50 ℃, performing second pile turning after the pile turning and continuing fermentation for 10 days, then continuing fermentation for 10 days, transferring to an aging tank, aging for 25 days, crushing by a crusher, and sieving to obtain an organic fertilizer;
(4) And (3) uniformly mixing the organic fertilizer obtained in the step (3) with bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus thuringiensis, beauveria bassiana, metarhizium anisopliae and potassium sulfate to obtain the special fertilizer for preventing and treating the black spot and the white grubs of the sweet potatoes.
2.2 application testing
The implementation time is as follows: in 2021.
Test site: liu Chaicun of Fuyang Dai City brick township, anhui province.
The soil nutrient status is as follows: the pH value is 7.2, the alkaline hydrolysis nitrogen is 98.4mg/kg, the available phosphorus is 35.70mg/kg, the quick-acting potassium is 145.00mg/kg, and the organic matter content is 21.6g/kg.
Sweet potato variety: watermelon red.
Test protocol: the experiment was set up in 5 groups of 3 replicates:
treatment (1): blank control, no fertilizer application;
treatment (2): the local fertilizer application is carried out by selecting the conventional compound fertilizer with the nutrient content of N-P 2 O 5 -K 2 O 5 The dosage is 15-15-15, and 40kg per mu;
treatment (3): on the basis of local habitual fertilization, 1kg of bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus thuringiensis, beauveria bassiana and metarhizium anisopliae are added in each mu;
treatment (4): the special biological fertilizer prepared in the embodiment 2 is used in an amount of 300 kg/mu;
treatment (5): the special biological fertilizer prepared in the embodiment 3 is used in an amount of 300 kg/mu,
the field management of each group is consistent.
1. The disease investigation method comprises the following steps: investigating the incidence condition of the black spot of the sweet potato in the potato block expanding period, sampling at 5 random points each time, 10 plants each time, and observing the incidence condition of roots; grading the grading standard of the diseased plants into 6 grades: grade 0, no scab; grade 1, with a few lesions; grade 2, the ratio of the lesion spots to the total plants is less than 25 percent; grade 3, the disease spots account for 25 to 50 percent of the total plant; 4-Fu grade, the disease spots account for 51-75% of the total plant proportion; grade 5, the ratio of the disease spots to the total plants is more than 75 percent
Incidence rate = (number of diseased potato pieces/number of investigated potato pieces) × 100%
Disease control effect = [ (control incidence-treatment incidence)/control incidence ] × 100%
2. The insect pest investigation method comprises the following steps: at harvest, 400 potato piece samples were randomly picked for statistics per treatment. The insect bite level of the potato blocks is graded according to the following standard: level 0, no insect bite, smooth surface of the potato blocks; grade 1, 1-2 insect-biting holes are formed, the insect-biting depth is shallow, the surfaces of the potato pieces are smooth, and the commodity rate of the potato pieces is not influenced; 2, 3-4 insect biting holes are formed, the insect biting depth is medium, obvious insect biting traces can be seen on the surfaces of the potato blocks, and the commodity rate of the potato blocks is influenced; grade 3, more than 5 insect-biting holes and deeper insect-biting depth seriously affect the commodity rate of the potato blocks.
Insect bite rate = [ (number of potato pieces at level 1 + number of potato pieces at level 2 + number of potato pieces at level 3)/investigation potato piece number ] × 100%
Pest control effect = [ (control insect bite rate-treated insect bite rate)/control insect bite rate ] × 100%
The test results are shown in table 3:
table 3: results of control tests with different treatments
As can be seen from table 3, in examples 2 and 3 of the present invention, the control effect on melasma and grub is significant during the sweet potato planting process, and the control effect is more than 75% compared with the control.
3. The soil improvement investigation method comprises the following steps: soil sampling is respectively carried out before sweet potato planting and before sweet potato harvesting, after sampling is carried out according to a 5-point method in each treatment, 1 sample is mixed, the sampling depth is 20cm, and the change conditions of the soil pH value of a plough layer, organic matters, nitrogen, phosphorus and potassium content and total content of microorganisms are measured.
Table 4: experimental conditions for soil improvement with different treatments
As can be seen from table 4, examples 2 and 3 of the present invention have significant effects on soil improvement, and have significant improvements in organic matter, total microbial count, and soil volume weight as compared to the control.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The biological special fertilizer for preventing and treating diseases and insect pests of the black spots of the sweet potatoes is characterized by comprising the following components in parts by weight: 75-85 parts of organic fertilizer, 1-2 parts of bacillus amyloliquefaciens, 1-2 parts of paenibacillus polymyxa, 1-2 parts of bacillus thuringiensis, 1-2 parts of beauveria bassiana, 1-2 parts of metarhizium anisopliae and 10-15 parts of potassium sulfate; the organic fertilizer is prepared from the following raw materials: 60-80 parts of cattle and sheep manure, 15-30 parts of straw, 5-10 parts of PAL material and 1-3 parts of zymophyte.
2. The organic fertilizer for preventing and treating sweet potato black spot diseases and insect pests as claimed in claim 1, is characterized in that: the fermentation inoculum comprises the following strains: bai Jianhui Streptomyces, saccharomyces cerevisiae, bacillus subtilis, bacillus thermophilus and Trichoderma viride.
3. The bio-organic fertilizer for controlling sweet potato black spot diseases and insect pests as claimed in claim 2, which is characterized in that: the mass ratio of the Bai Jianhui streptomyces, saccharomyces cerevisiae, bacillus subtilis, bacillus thermophilus and trichoderma viride is 5.
4. The preparation method of the biological special fertilizer for controlling sweet potato black spot diseases and insect pests as claimed in any one of claims 1 to 3, which is characterized by comprising the following steps:
(1) Crushing corn straws or wheat straws into powder, uniformly mixing the crushed straws with cattle and sheep excrement to obtain a mixed material, adding a zymophyte agent into the mixed material, uniformly mixing, adjusting the water content of the mixed material to be 60-70%, performing composting, decomposing and fermenting, crushing by a crusher after fermentation is completed, and sieving to obtain an organic fertilizer;
(2) And uniformly mixing the organic fertilizer with bacillus amyloliquefaciens, paenibacillus polymyxa, bacillus thuringiensis, beauveria bassiana, metarhizium anisopliae and potassium sulfate to obtain the special fertilizer for preventing and treating the black spot disease and the grubs of the sweet potatoes.
5. The method of claim 4, wherein: the composting, decomposing and fermenting in the step (1) comprises the following specific steps: performing first pile turning after composting for 10 days, wherein the fermentation temperature is above 50 ℃, continuing to ferment for 10 days after pile turning, performing second pile turning, continuing to ferment for 10 days, transferring to an aging pool, aging for 25 days, and completing fermentation.
6. The application of the special biological fertilizer for controlling the sweet potato black spot diseases and insect pests as claimed in any one of claims 1 to 3 in controlling sweet potato black spots and grubs and improving soil.
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2022
- 2022-10-08 CN CN202211229379.4A patent/CN115433029A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103274825A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special polyelement microorganism fertilizer for preventing celery soft rot and preparation method of special polyelement microorganism fertilizer |
| CN107027482A (en) * | 2017-04-26 | 2017-08-11 | 吉林省彬生农业科技发展有限公司 | A kind of implantation methods for being remarkably improved lysine content in quinoa seedling |
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