KR102511152B1 - Bacillus siamensis LES120 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT - Google Patents

Abstract

The present invention provides a Bacillus siamensis LES120 strain (KACC 81133BP) showing a control effect on foot blight caused by Ralstonia solanacearum pathogens and a microbial preparation containing the same, Establish technology that can control green blight in an eco-friendly way and produce crops in an eco-friendly way.

Description

Bacillus siamensis LES120 strain showing control effect against bacterial blight {Bacillus siamensis LES120 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT}

The present invention relates to a Bacillus siamensis LES120 strain (KACC 81133BP) exhibiting a control effect on foot blight caused by Ralstonia solanacearum pathogens and a microbial preparation comprising the same .

Global surface temperatures have been continuously rising over the past century and are projected to rise between 1.0°C and 4.8°C by the end of the century (IPCC, 2014). An increase in global average temperature is one of the major abiotic stresses that plants must overcome, causing an increase in the occurrence of plant diseases. In Korea, as the subtropical climate is moving north, it is predicted that the occurrence of thermophilic diseases of major domestic crops will intensify.

Red pepper is one of the five major vegetables in Korea, and the amount of red pepper production is expected to be KRW 1,144.9 billion in 2018 and KRW 1,185.1 billion in 2019, which ranks ninth among the total production of agricultural, livestock and livestock products. In 2017, pepper production decreased by 35% from the previous year due to drought in the early stages of growth, high temperature in summer, and disease spread due to frequent rainfall. It was 5% lower than normal due to drought and drought.

Green pepper blight is caused by the bacterial pathogen Ralstonia solanacearum and occurs throughout the growing season from planting to harvesting every year. Ralstonia solanacearum species complex (RSC) is a major bacterial pathogen responsible for massive yield losses in more than 200 plant species in tropical, subtropical and temperate regions worldwide. Ralstonia solanacearum ( R. solanacearum ) Pepper green blight caused by onset rarely progresses below 20 ° C, and onset is severe under high temperature conditions. Even in Korea, where summer temperatures are gradually rising, damage from foot blight caused by Ralstonia solanacearum ( R. solanacearum ) is on the rise, and general farmhouses raise the dike to ensure good drainage or remove the blight immediately. There is no effective control method.

As chemical pesticides to control green pepper blight in Korea, Dazomet and metam-sodium, which can only be treated before planting seedlings, are registered. Oxytetracycline has been registered as a chemical agent that can be treated during the growing season of tomatoes belonging to the solanaceae genus such as pepper, but the control effect rapidly decreased over time when treated with pepper. In addition, oxytetracycline is a tetracycline-based antibiotic, and resistance is expressed in various bacteria through horizontal transfer of resistance genes present in plasmids or transposons between bacteria. reported Since Ralstonia solanacearum ( R. solanacearum ) causes green blight of pepper due to lack of microbial agents to control it, it is difficult to grow environmentally friendly, so it is necessary to develop control microorganisms having preventive and therapeutic effects.

The present invention has been made to solve the problems of the prior art as described above, has a green blight inhibitory effect, is low in toxicity, safe for humans and animals, and has no soil and water pollution problems, contributing to the production of high-quality crops in eco-friendly farms It is an object to provide a composition for controlling foot blight that can be.

The present invention provides a Bacillus siamensis LES120 strain (KACC 81133BP) for controlling plant blight.

In one embodiment of the present invention, the foot blight may be caused by the Ralstonia solanacearum pathogen.

In one embodiment of the present invention, the strain may include the nucleotide sequence of SEQ ID NO: 1.

In one embodiment of the present invention, the plant may be a solanaceous crop including pepper, tomato, eggplant, and tobacco.

In one embodiment of the present invention, the strain may include at least one of a microbial pesticide, a seed coating agent, a soil improving agent, a compost composting agent, a foliar spreading agent, and a drenching agent.

The present invention provides a microbial preparation comprising Bacillus siamensis LES120 strain (KACC 81133BP) for controlling plant blight, spores of the strain, culture broth of the strain, or a mixture thereof as an active ingredient.

In one embodiment of the present invention, the microbial agent may include at least one of a microbial pesticide, a seed coating agent, a soil improving agent, a compost composting agent, a foliar spreading agent, and a drenching agent.

The present invention is the step of preparing the Bacillus siamensis LES120 strain (KACC 81133BP), and inoculating the strain into TSB (Tryptic Soy Broth) medium, and then 20 ~ 35 ℃ 100 ~ 200 rpm under 40 ~ 200 rpm conditions It provides a method for producing a microbial agent for controlling plant blight, comprising culturing for 50 hours.

The present invention is a Bacillus siamensis LES120 strain (KACC 81133BP) strain, and at least one of the microbial preparations prepared using the strain as an active ingredient, a fertilizer for controlling plant blight to provide.

The present invention provides a method for increasing the resistance of plants to green blight by drenching the soil with the above fertilizer.

Using the Bacillus siamensis LES120 strain according to the present invention, a technology capable of environmentally friendly control of foot blight is established, and crops can be produced in an environmentally friendly manner.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of Bacillus siamensis LES120 strain.
Figure 2 is a graph showing the amount (CFU / g) of strains of bacteria isolated from the rhizosphere and endosphere of Ligularia fischeri cultured in TSA medium and NA medium, respectively.
Figure 3a shows pepper seedlings tested for the green blight control effect of the LES120 strain culture medium on pepper seedlings.
Figure 3b shows pepper seedlings tested for the effect of controlling green blight of IC (Inoculated control; pathogen inoculation control) on pepper seedlings.
Figure 4 is a diseased state rate of pepper seedlings treated with a culture medium of LES120 strain, Serenade, or untreated (IC (Inoculated control; pathogen inoculated control) and UT (Untreated control; pathogen uninoculated control)) This is a table showing the plant.
Figure 5 shows the plant height of pepper seedlings treated with LES120 strain culture, Serenade, or untreated (IC (Inoculated control; inoculated control) and UT (Untreated control; non-inoculated control)) is the table shown.

Since the present invention can make various changes and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, the embodiments disclosed in this specification will be described in detail with reference to the drawings. The illustrative embodiments described above in the detailed description, drawings and claims are not intended to be limiting, and other embodiments than those described may be used, and other changes may be made without departing from the spirit or scope of the technology disclosed herein. are also possible

Since the description of the disclosed technology is only an embodiment for structural or functional description, the scope of rights of the disclosed technology should not be construed as being limited by the embodiment described in the text. That is, since the embodiment can be changed in various ways and can have various forms, it should be understood that the scope of rights of the disclosed technology includes equivalents capable of realizing the technical idea.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed technology belongs, unless defined otherwise. Terms defined in commonly used dictionaries should be interpreted as consistent with meanings in the context of related art, and cannot be interpreted as having ideal or excessively formal meanings unless explicitly defined in the present application.

Green blight (bacterial wilt) is a disease caused by the Ralstonia solanacearum pathogen, which causes severe damage to major solanaceous crops such as tomatoes, peppers, eggplants, and tobacco. In addition, it can act as a potential hazard to almost all horticultural crops such as fruits and vegetables, leafy vegetables, and spinach, so the risk of damage is very high. In particular, the frequency of occurrence is gradually increasing according to the recent trend of agriculture in which continuous cultivation and year-round cultivation of the same crop are prevalent. Almost all crops cannot be harvested because the whole is dying.

Chemical agents can be used as a means to control blight, but Dazomet and Metam-sodium, which can be treated only before planting seedlings, have been registered as chemical pesticides for blight control in Korea. , Treatment is not possible during the cultivation of pepper., Oxytetracycline has been registered as a control agent that can be treated during the cultivation of tomatoes. Rather, resistance was expressed through horizontal movement of resistance genes between bacteria.

If the above chemical pesticides and chemical fertilizers are used excessively for plant disease control, problems such as soil contamination, repeated crop failure, soil nutrient imbalance, and disease and pest occurrence occur, resulting in a decrease in product quality and farmer's income. side effects such as.

Accordingly, the present invention provides a Bacillus siamensis LES120 strain, a culture solution of the strain, or a microbial agent containing at least one of them, which exhibits a control effect on plant blight by a biological method. The plants may be, for example, major solanaceous crops such as tomatoes, peppers, eggplants, and tobacco, and may also be horticultural crops such as fruits and vegetables, leafy vegetables, and wraps.

The foot blight is caused by Ralstonia solanacearum pathogens or Pseudomonas solanacearum pathogens.

In the present specification, "culture medium" is a result obtained by inoculating bacteria into a medium and culturing for a certain period of time, the culture medium itself in which the bacteria according to the present invention are cultured in a suitable liquid medium, and the filtrate obtained by filtering or centrifuging the culture medium to remove bacteria (filtration solution or centrifuged supernatant), a cell disruption solution obtained by sonicating the culture solution or treating the culture solution with a lysozyme, and a concentrate obtained by concentrating the culture solution, filtrate, or the cell disruption solution, etc. It is not limited. In addition, the probiotic or antibacterial composition may contain a culture such as a solid medium culture, or a dried product or extract of the culture medium, but is not limited thereto, and further suitable excipients or carriers (recessive gene in a heterozygous state) possessed object) may be additionally included.

The microbial preparation of the present invention may be prepared in the form of a liquid fertilizer, and may be used in the form of a powder by adding an extender thereto, or may be formulated and granulated. However, the dosage form is not particularly limited, and it may be prepared in a ring form other than liquid or powder.

The present invention provides a method for controlling plant blight by using a Bacillus siamensis LES120 strain, a culture solution of the strain, or a microbial agent containing at least one of them.

In the present invention , Bacillus siamensis LES120 strain was deposited with the National Academy of Agricultural Sciences as of August 26, 2020, and was given KACC 81133BP as an accession number.

The 16S rRNA base sequence of the strain is represented by SEQ ID NO: 1.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of Bacillus siamensis LES120 strain.

In one embodiment of the present invention, Bacillus siamensis LES120 strain (KACC 81133BP) is used as at least one active ingredient among microbial pesticides, seed coating agents, soil improving agents, compost composting agents, foliar spreading agents, and drenching agents It could be.

Bacillus siamensis ( Bacillus siamensis ) The microbial preparation prepared using the LES120 strain (KACC 81133BP) is the LES120 strain, the culture medium of the LES120 strain, or a mixture thereof, any other used to control green blight in the field It may further include the components of. However, it is not limited thereto, and may further include, for example, a plant nutrient, a plant growth regulator, a plant growth stimulant, and a plant disease control agent capable of controlling other plant diseases. In addition, at least one or more of herbicides, fungicides, insecticides, seed coating agents, soil improvers, compost composting agents, foliar spreading agents, drenching agents, compost, and fertilizers may be further included.

The plant growth regulator may include other beneficial microorganisms, microbial activators and organic fertilizers. However, it is not limited thereto, and may further include a chemical fertilizer.

The microbial active material may include at least one or more of enzyme precursors, microbial metabolites, organic acids, carbohydrates, enzymes, and trace elements. For example, microbial active substances include processed enzyme products such as yeast autolysates, humic (non-terrestrial and subsurface organic matter) materials, seaweed extracts, starch, amino acids, and zinc, iron, copper, manganese, Trace elements such as bromine and molybdenum may be included. However, it is not limited to the trace elements.

The plant growth regulator or plant growth stimulant is a Bacillus spp., Azotobacter spp., Trichoderma spp., and Saccharomyces, known to promote plant growth. Genus ( Saccharomyces spp.) At least one or more strains may be included. However, it is not limited to the microbial species, Streptomyces genus ( Streptomyces sp . ), Rhizobium genus ( Rhizobium sp . ), Pseudomonas genus ( Pseudomonas sp . ) It may further include strains.

Other beneficial microorganisms, microbial activators and organic fertilizers may be the same as described above. Chemical fertilizers can include a variety of chemicals that can provide nitrogen, phosphorus, and potassium nutrients to support plant growth. For example, chemical fertilizers may include urea, calcium phosphate, potassium phosphate and mixed nitrogen-phosphate-potassium (N-P-K) fertilizers. However, it is not limited thereto, and the chemical fertilizer may also include other materials known in the field (carbon, hydrogen, oxygen, sulfur, lime, goto, boron, iron, manganese, zinc, copper, molybdenum, chlorine, etc.) .

Hereinafter, the present invention will be described in more detail through experimental examples. These experimental examples are only for exemplifying the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these experimental examples.

<Experimental Example 1> Strain isolation and culture method

To separate microorganisms from Gomchiw, Ligularia fischeri rhizosphere soil, 3 g of rhizosphere soil was added to 27 ml of sterilized water, shaken at 28 ° C. at 180 rpm for 30 minutes, and 1 ml was taken and suspended in 9 ml of sterilized water. . In order to isolate microorganisms from the roots, the roots of bear sting were surface sterilized and then crushed with a sterilized mortar and pestle, and 1 g of the root was suspended in 9 ml of sterile water. The rhizosphere soil and root suspension were diluted in stages, spread on TSA (Tryptic Soybean Agar) medium and NA (Nutrient Agar) medium, and cultured at 28 ° C for 48 hours. (glycerol) and stored at -70 ° C and used for experiments.

The isolated strain is Bacillus siamensis LES120 strain, and has a 16S rRNA nucleotide sequence represented by SEQ ID NO: 1.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of Bacillus siamensis LES120 strain.

Figure 2 is a graph showing the amount (CFU / g) of strains of bacteria isolated from the rhizosphere and endosphere of Ligularia fischeri cultured in TSA medium and NA medium, respectively.

The strain cultured in rhizosphere using NA medium is 3.7 X 10 ⁶ CFU/g, and the strain cultured in rhizosphere using TSA medium is 6.8 X 10 ⁶ CFU/g. The strain cultured using the NA medium in the endosphere is 1.6 X 10 ⁴ CFU/g, and the strain cultured in the endosphere using the TSA medium is 3.7 X 10 ⁴ CFU/g.

Among the above strains, it was confirmed that the strain having the most excellent control effect against green blight was Bacillus siamensis LES120 strain, and the LES120 strain was finally selected.

The LES120 strain has a 16S rRNA nucleotide sequence represented by SEQ ID NO: 1.

<Experimental Example 2> Test of antagonistic ability of the isolated strain against the reverse pathogen

The antagonism of the isolates against Phytophthora capsici and Phytophthora drechsleri was tested three times. The isolated strain was smeared at a distance of 35 mm from the center of a PDA (Potato Dextrose Agar) medium and cultured at 25° C. for 24 hours, and sterile water was used for the control. Phytophthora capsaishi ( P. capsici ) and Phytophthora drechsleri ( P. drechsleri ) colonies were placed in the center of each PDA medium, incubated at 25 ° C. for 5 days, and then incubated in the low zone (Inhibition zone) Microorganisms with excellent antibacterial activity were selected by measuring the size of.

Table 1 is a table showing the antifungal activity of the LES120 strain against Phytophthora capsici , which causes root rot in various crops (including pepper plants) using a double replacement culture assay.

Treatment Mycelial growth inhibition rate (%)* Mean of mycelial growth inhibition rate (%) standard error ^1st trial ^2nd trial ^3rd trial LES120 27.0 31.0 28.7 28.9 0.95 Control** 0.0 0.0 0.0 0.0 0.0

* (Control cell diameter (mm) - Treatment cell diameter (mm)) / Control cell diameter (mm)) X100 **Control is treated with sterile distilled water. The cell diameter of the control is 68.4 mm.

Table 2 is a table showing the antifungal activity of the LES120 strain against Phytophthora drechsleri that causes root rot in various crops (including pepper plants) using a double replacement culture assay.

Treatment Micelial growth inhibition rate (%)* Mean of mycelial growth inhibition rate (%) standard error ^1st trial ^2nd trial ^3rd trial LES120 31.0 30.4 33.7 31.7 0.84 Control** 0.0 0.0 0.0 0.0 0.0

* (Control cell diameter (mm) - Treatment cell diameter (mm)) / Control cell diameter (mm)) X100 **Control is treated with sterile distilled water. The cell diameter of the control is 61.3 mm.

<Experimental Example 3> Examination of the effect of controlling green blight by treatment of the culture solution of the finally selected LES120 strain

Bacillus siamensis ( Bacillus siamensis ) Green blight of LES120 strain ( Ralstonia solanacearum ) In order to test the control effect on, a bioassay was performed using pepper seedlings in a greenhouse.

First, the LES120 strain was smeared on TSA medium and cultured at 28° C. for 24 hours. Thereafter, a single colony of the cultured LES120 strain was inoculated into TSB (Tripic Soy Broth) medium and cultured at 28° C. at 150 rpm for 48 hours, and then the concentration of the culture medium was adjusted to 1.0X10 ⁸ CFU/mL (OD ₆₀₀ =0.25 ).

Pepper seeds (Super manitta, Nongwoo-bio, Korea) were washed with flowing tap water for 2 hours, sterilized the surface using 1% sodium hypochlorite and 70% ethanol to remove microorganisms on the surface, and then potted with a diameter of 10 cm. Top soil (Green soil, Seoul Bio, Korea) was added and seeded (n = 15).

After 4 weeks of sowing, pepper seedlings with 7 to 8 true leaves were treated with 50mL of the culture solution of the isolated microorganism LES120 strain, and after 7 days, the green blight Ralstonia solanacearum ( Ralstonia solanacearum ) 20mL per week at a concentration of 1.0X10 ⁸ CFU/mL (OD ₆₀₀ =0.15) was treated with irrigation. After 5 weeks of treatment with the Ralstonia solanacearum strain, disease incidence (%) and plant height were investigated.

Figure 3a shows pepper seedlings tested for the green blight control effect of the LES120 strain culture medium on pepper seedlings, Figure 3b shows the pepper seedlings tested for the green blight control effect of IC (Inoculated Control) on pepper seedlings .

In the case of the pepper seedlings of FIG. 3a treated with the culture solution of the LES strain to control blight, blight rarely occurred, whereas only pathogens were inoculated without treating the strain for blight control (IC (Inoculated Control)) In this case, the effect of controlling green blight was significantly reduced. In the case of the pepper seedlings of FIG. 3b, the inside of the stem of the pepper seedlings was brown, and the green blight control effect was significantly lower than that of the pepper seedlings of FIG. 3a.

4 is SEL5, SEL1, SEL3, YP2, HC37, SB19, CeR16-2, RIR15, ObRS-5, ObRS-6, ObRS-15, HLS-9, ObBS-4, GJ6-4, LES4, When cultures of LES37, LES58, LES61, LES88, LES93, LES118-1, LES118-2, LES120 strains were treated or untreated (IC (Inoculated control; pathogen inoculation control) and UT (Untreated control; pathogen uninoculated control) )) is a table showing the diseased plant rate.

The test was performed in duplicate. In the first trial, when pepper seedlings were treated with the culture solution of strain LES120, 0% of green blight disease was observed. The IC (Inoculated control) showed a morbidity rate of 63.3%, and the UT (Untreated control) showed no morbidity rate. When pepper seedlings were treated with the culture solution of strain LES120, the effect of controlling green blight was 100%. In the second trial, when seedlings of pepper were treated with the culture solution of strain LES120, a 20.0% rate of blight was observed. IC (Inoculated control) showed 100% morbidity rate, and UT (Untreated control) showed no morbidity rate. When seedlings of pepper were treated with the culture solution of strain LES120, the control effect of green blight was 80%, indicating a significant control effect.

Figure 5 shows the plant height of pepper seedlings treated with LES120 strain culture, Serenade, or untreated (IC (Inoculated control; inoculated control) and UT (Untreated control; non-inoculated control)) is the table shown.

The test was performed in duplicate. In the first experiment, when seedlings of pepper were treated with the culture solution of strain LES120, plant height of 24.6 cm was shown. In the case of serenade treatment, the plant height was 23.6 cm, in the case of IC (Inoculated control), 21.5 cm, and in the case of UT (Untreated control), 22.3 cm.

In the second experiment, when seedlings of pepper were treated with the culture solution of strain LES120, plant height of 33.8 cm was shown. In the case of Serenade treatment, the plant height was 31.6 cm, IC (Inoculated control), 28.4 cm, and UT (Untreated control), 29.8 cm. . In other words, the fact that the change in plant height of pepper seedlings by treatment with LES120 strain was insignificant through two repeated experiments can be interpreted as the fact that treatment with LES120 strain can effectively control green blight without inhibiting the growth of pepper.

The above present invention has been examined with a focus on preferred experimental examples, and those skilled in the art can implement embodiments of a different form from the detailed description of the present invention within the essential technical scope of the present invention. You will be able to. Here, the essential technical scope of the present invention is shown in the claims, and all differences within the equivalent range should be construed as being included in the present invention.

National Institute of Agricultural Science Agricultural Genetics Center KACC81133BP 20200923

<110> REPUBLIC OF KOREA, REPUBLIC OF KOREA <120> Bacillus siamensis LES120 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT <130> FP-2009045-KR <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1347 <212> RNA <213> unknown <220> <223> Bacillus siamensis LES120 <400> 1 gctgatccgc gattactagc gattccagct tcacgcagtc gagttgcaga ctgcgatccg 60 aactgagaac agatttgtgg gattggctta acctcgcggt ttcgctgccc tttgttctgt 120 ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac gtcatcccca 180 ccttcctccg gtttgtcacc ggcagtcacc ttagagtgcc caactgaatg ctggcaacta 240 agatcaaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac gagctgacga 300 caaccatgca ccacctgtca ctctgccccc gaaggggacg tcctatctct aggattgtca 360 gaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca catgctccac 420 cgcttgtgcg ggcccccgtc aattcctttg agtttcagtc ttgcgaccgt actccccagg 480 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac 540 tcatcgttta cggcgtggac taccagggta tctaatcctg ttcgctcccc acgctttcgc 600 tcctcagcgt cagttacaga ccagagagtc gccttcgcca ctggtgttcc tccacatctc 660 tacgcatttc accgctacac gtggaattcc actctcctct tctgcactca agttccccag 720 tttccaatga ccctccccgg ttgagccggg ggctttcaca tcagacttaa gaaaccgcct 780 gcgagccctt tacgcccaat aattccggac aacgcttgcc acctacgtat taccgcggct 840 gctggcacgt agttagccgt ggctttctgg ttaggtaccg tcaaggtgcc gccctatttg 900 aacggcactt gttcttccct aacaacagag ctttacgatc cgaaaacctt catcactcac 960 gcggcgttgc tccgtcagac tttcgtccat tgcggaagat tccctactgc tgcctcccgt 1020 aggagtctgg gccgtgtctc agtcccagtg tggccgatca ccctctcagg tcggctacgc 1080 atcgtcgcct tggtgagccg ttacctcacc aactagctaa tgcgccgcgg gtccatctgt 1140 aagtggtagc cgaagccacc ttttatgtct gaaccatgcg gttcagacaa ccatccggta 1200 ttagccccgg tttcccggag ttatcccagt cttacaggca ggttacccac gtgttactca 1260 cccgtccgcc gctaaca gggagcaagc tcccatctgt ccgctcgact tgcatgtatt 1320 aggcacgccg ccagcgttcg tcctgag 1347

Claims (10)

Ralstonia solanacearum ( Ralstonia solanacearum ) For controlling green blight caused by pathogens and for controlling Phytophthora drechsleri ( Phytophthora drechsleri ) Bacillus siamensis for controlling root rot caused by pathogens Bacillus siamensis LES120 strain ( KACC 81133BP). delete According to claim 1,
The strain is Bacillus siamensis LES120 strain (KACC 81133BP) containing the nucleotide sequence of SEQ ID NO: 1. According to claim 3,
The target plant for controlling green blight is Bacillus siamensis LES120 strain (KACC 81133BP), which is a solanaceous crop including pepper, eggplant, and tobacco. According to claim 4,
The strain is a microbial pesticide, a seed coating agent, a soil conditioner, a compost composting agent, a foliar spraying agent, and a drenching agent, including at least one of Bacillus siamensis LES120 strain (KACC 81133BP). Ralstonia solanacearum ( Ralstonia solanacearum ) For controlling green blight caused by pathogens and for controlling Phytophthora drechsleri ( Phytophthora drechsleri ) Bacillus siamensis for controlling root rot caused by pathogens Bacillus siamensis LES120 strain ( KACC 81133BP), spores of the strain, a culture broth of the strain, or a mixture thereof as an active ingredient. According to claim 6,
The microbial agent is a microbial agent comprising at least one of a microbial pesticide, a seed coating agent, a soil improving agent, a compost composting agent, a foliar spraying agent, and a drenching agent. Preparing a Bacillus siamensis LES120 strain (KACC 81133BP) according to any one of claims 1, 3 to 5; and
After inoculating the strain in TSB (Tryptic Soy Broth) medium, incubating for 40 to 50 hours under 20 ~ 35 ℃ 100 ~ 200 rpm conditions; Method for producing a microbial agent for controlling green blight of plants. At least one of the cells of the Bacillus siamensis LES120 strain (KACC 81133BP) according to any one of claims 1 and 3 to 5, and a microbial preparation prepared using the strain A fertilizer for controlling plant blight, containing as an active ingredient. A method for increasing the resistance of plants to green blight by drenching the soil with the fertilizer according to claim 9.

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