KR102448561B1 - Cytobacillus solani LES88 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT - Google Patents

Abstract

The present invention Ralstonia solanacearum ( Ralstonia solanacearum ) Cytobacillus solani ( Cytobacillus solani ) LES88 strain (KACC 81134BP), which exhibits a control effect on blight caused by pathogens, and a microbial preparation comprising the same Establish a technology that can control green blight in an environment-friendly way, and make it possible to produce crops in an environment-friendly way.

Description

Cytobacillus solani LES88 strain showing a control effect on bacterial blight {Cytobacillus solani LES88 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT}

The present invention relates to Ralstonia solanacearum ( Ralstonia solanacearum ) Cytobacillus solani showing a control effect on blight caused by pathogens ( Cytobacillus solani ) LES88 strain (KACC 81134BP) and a microbial preparation comprising the same .

Global surface temperature has been rising continuously over the past century and is expected to rise from 1.0°C to 4.8°C by the end of the century. An increase in the global average temperature is one of the major abiotic stresses that plants must overcome, causing an increase in the incidence of plant diseases. As the subtropical climate is moving northward in Korea, it is predicted that the occurrence of thermophilic diseases of major domestic crops will intensify.

Red pepper is one of the five largest vegetables in Korea, and the production value of red pepper is expected to reach KRW 1144.9 billion in 2018 and KRW 1185.1 billion in 2019, ranking 9th among the total agricultural, forestry and livestock food production. Pepper production in 2017 decreased by 35% compared to the previous year due to the spread of diseases caused by drought in the early stages of growth, high temperature in summer, and frequent rainfall (Kim et al., 2017). %, but it was 5% lower than the average year due to summer heat and drought.

The blight that occurs in red pepper is caused by the bacterial pathogen Ralstonia solanacearum , and it develops throughout the entire growing season from planting to harvest every year. Ralstonia solanacearum species complex (RSC) is a major bacterial pathogen that causes massive yield loss in more than 200 plant species in tropical, subtropical and temperate regions around the world. Red pepper blight caused by Ralstonia solanacearum ( R. solanacearum ) hardly progresses below 20 ℃, and the onset is severe at high temperature conditions. Even in Korea, where the summer temperature is rising, the damage caused by R. solanacearum is on the rise, and general farms are raising their ridges to make drainage better or to immediately remove the disease, but it is an effective control method. is not present.

As chemical pesticides for controlling red pepper blight in Korea, Dazomet and metam-sodium, which can be treated only before seedling planting, are registered. Oxytetracycline is registered as a chemical agent that can be treated during the small period of tomatoes belonging to the Solanaceae genus, such as red pepper. In addition, oxytetracycline is a tetracycline-based antibiotic, and resistance is expressed in a variety of bacteria through horizontal movement of resistance genes present in plasmids or transposons. has been reported Ralstonia solana serum ( R. solanacearum ) Red pepper blight caused by the lack of a microbial agent for controlling it is difficult in environment-friendly cultivation, so it is necessary to develop a control microorganism having a preventive and therapeutic effect.

The present invention has been devised to solve the problems of the prior art as described above, has a blight inhibitory effect, has low toxicity, is safe for livestock, and has no soil and water pollution problems. It is an object to provide a composition for controlling blight that can be.

The present invention provides a Cytobacillus solani LES88 strain (KACC 81134BP) for controlling blight of plants.

In one embodiment of the present invention, the blight may be caused by a Ralstonia solanacearum pathogen.

In one embodiment of the present invention, the strain may include the nucleotide sequence of SEQ ID NO: 1.

In one embodiment of the present invention, the plant may be a solanaceae crop including pepper, tomato, eggplant, and tobacco.

In one embodiment of the present invention, the strain can be used as an active ingredient of any one of microbial pesticides, seed coating agents, soil improvers, composting agents, foliar spreaders, and irrigation sprayers.

The present invention provides a microbial preparation comprising, as an active ingredient, a Cytobacillus solani LES88 strain (KACC 81134BP), a spore of the strain, a culture solution of the strain, or a mixture thereof for controlling plant blight.

In one embodiment of the present invention, the microbial preparation may include any one or more of a microbial pesticide, a seed coating agent, a soil improver, a composting agent, a foliar spreader, and a irrigation spraying agent.

The present invention is the Cytobacillus solani ( Cytobacillus solani ) After preparing the LES88 strain (KACC 81134BP), and inoculating the strain in TSB (Tryptic Soy Broth) medium, 20 ~ 35 ℃ 40 ~ under 100 ~ 200 rpm conditions It provides a method for producing a microbial agent for controlling plant blight, comprising the step of culturing for 50 hours.

The present invention provides a fertilizer for controlling blight of plants, comprising, as an active ingredient, any one or more of the cells of the Cytobacillus solani LES88 strain (KACC 81134BP) or a microbial preparation prepared using the strain as an active ingredient do.

The present invention provides a method of increasing the resistance of plants to blight by irrigating the soil with the fertilizer.

Cytobacillus solani according to the present invention ( Cytobacillus solani ) By using the LES88 strain to build a technology that can control the green blight in an environment-friendly way, and to produce crops in an environment-friendly way.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of the Cytobacillus solani ( Cytobacillus solani ) LES88 strain.
Figure 2 is a graph showing the amount (CFU / g) of bacteria isolated from the rhizosphere and the inner sphere (Endosphere) of Gomchwi (Ligularia fischeri) cultured in TSA medium and NA medium, respectively.
Figure 3a shows the pepper seedlings tested for the blight control effect of the culture medium of the LES88 strain on the pepper seedlings.
Figure 3b shows the pepper seedlings tested for the blight control effect of IC (Inoculated control; pathogen inoculated control) on the pepper seedlings.
Figure 4 is a culture medium of the LES88 strain in pepper seedlings, serenade (Serenade) treated or untreated (IC (Inoculated control; pathogen inoculated control) and UT (Untreated control; pathogen non-inoculated control)) of the diseased strain rate (Diseased) plant).
Figure 5 is in order to evaluate the effect of the culture medium of the LES88 strain on the pepper growth, the culture medium of the LES88 strain on the pepper seedlings, serenade (Serenade), or untreated (IC (Inoculated control; pathogen inoculation control) and UT) (Untreated control; non-inoculation control with pathogens)) is a table showing the plant height (Plant height).

Since the present invention can have various changes and can have various embodiments, specific embodiments are illustrated in the drawings and will be described in detail in the text. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of a related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.

Hereinafter, embodiments disclosed in the present specification will be described in detail with reference to the drawings. Exemplary embodiments described above in the detailed description, drawings, and claims are not for limitation, and embodiments other than the described embodiments may be used, and other changes may be made without departing from the spirit or scope of the technology disclosed herein. are also possible

Since the description of the disclosed technology is merely an embodiment for structural or functional description, the scope of the disclosed technology should not be construed as being limited by the embodiment described in the text. That is, since the embodiment may have various changes and may have various forms, it should be understood that the scope of the disclosed technology includes equivalents capable of realizing the technical idea.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed technology belongs, unless otherwise defined. Terms defined in the dictionary should be interpreted as being consistent with the meaning of the context of the related art, and cannot be interpreted as having an ideal or excessively formal meaning unless explicitly defined in the present application.

Green blight (bacterial wilt disease) is a disease caused by a Ralstonia solanacearum pathogen, which occurs in major solanaceae crops such as tomatoes, peppers, eggplants, and tobacco, causing severe damage. In addition, it can act as a potential hazard to almost all horticultural crops such as fruits and vegetables, leafy vegetables, and green vegetables, so the risk of damage is very high. In particular, the frequency of occurrence is gradually increasing according to the recent agricultural trend in which continuous and year-round cultivation of the same crop is prevalent. Almost all crops cannot be harvested because the whole has died.

Chemical agents can be used as a means to control blight, but Dazomet and Metam-sodium, which can be treated only before seedlings are planted, are registered as chemical pesticides for controlling blight in Korea. It cannot be processed while the pepper is small. Although oxytetracycline is registered as a control agent that can be treated during tomato planting, when it is treated with red pepper, as time passes after treatment, the blight control effect rapidly decreases, and Resistance expression was shown through enemy migration.

When chemical pesticides and chemical fertilizers are used excessively as described above to control plant diseases, problems such as soil contamination, failure of continuous cultivation, imbalance of soil nutrients, and occurrence of diseases and pests occur, resulting in a decrease in the quality of products and a decrease in the income of farmers side effects such as

Accordingly, the present invention provides a microbial preparation comprising at least one of a Cytobacillus solani LES88 strain and a culture solution of the strain, or at least one of them, which exhibits a control effect on blight of plants by a biological method. The plant may be, for example, a major solanaceae crop such as tomatoes, peppers, eggplants, and tobacco, and may be horticultural crops such as fruits and vegetables, leafy vegetables, and ssam vegetables.

The blight is caused by Ralstonia solanacearum pathogens or Pseudomonas solanacearum pathogens.

As used herein, the term “culture solution” refers to a result obtained by inoculating bacteria into a medium and culturing for a certain period of time. solution or centrifuged supernatant), a cell lysate obtained by sonicating the culture medium or treating the culture medium with a lysozyme, a concentrate obtained by concentrating the culture medium, the filtrate, the cell lysate, etc. It is not limited. In addition, the probiotic or antibacterial composition may contain a culture such as a solid medium culture, or a dried product or an extract of the culture medium, but is not limited thereto, and furthermore, a suitable excipient or carrier (the recessive gene in a state of heterozygosity) objects that you have) may be additionally included.

The microorganism preparation of the present invention may be prepared in the form of a liquid fertilizer, and it may be used in the form of a powder by adding an extender thereto, or it may be formulated and granulated. However, the formulation is not particularly limited, and it may be prepared in a ring form other than liquid or powder.

The present invention provides a method for controlling blight of plants using a microbial preparation comprising at least one of the Cytobacillus solani LES88 strain and the culture medium of the strain or these.

In the present invention, Cytobacillus solani ( Cytobacillus solani ) LES88 strain deposited microorganisms with the National Academy of Agricultural Sciences as of October 26, 2020, and was given KACC 81134BP as an accession number.

The 16S rRNA nucleotide sequence of the strain is represented by SEQ ID NO: 1.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of the Cytobacillus solani ( Cytobacillus solani ) LES88 strain.

In one embodiment of the present invention, Cytobacillus solani LES88 strain (KACC 81134BP) is used as an active ingredient of any one of microbial pesticides, seed coating agents, soil improvers, composting agents, foliar spraying agents or drenching sprayers. it might be

Cytobacillus solani ( Cytobacillus solani ) Microbial preparations prepared using the LES88 strain (KACC 81134BP) are the LES88 strain, the culture solution of the LES88 strain, or a mixture thereof Any other used as controlling blight blight in the art It may further include a component of. However, the present invention is not limited thereto, and may further include, for example, plant nutrients, plant growth regulators, plant growth stimulants, and plant disease control agents capable of controlling other plant diseases. In addition, it may further include at least one or more of herbicides, fungicides, pesticides, seed coating agents, soil conditioners, compost foliar spreaders, drench spreaders, composts, and fertilizers.

The plant growth regulator may include other beneficial microorganisms, microbial active substances and organic fertilizers. However, the present invention is not limited thereto, and may further include a chemical fertilizer.

The microbial active material may include at least one of enzyme precursors, microbial metabolites, organic acids, carbohydrates, enzymes and trace elements. For example, microbial active substances include processed enzyme products such as yeast autolysates, humus (organic substances other than those present on the surface and in the soil), seaweed extracts, starch, amino acids, and zinc, iron, copper, manganese, Trace elements such as bromine and molybdenum may be included. However, it is not limited to the above trace elements.

The plant growth regulator or plant growth stimulator is conventionally known to promote plant growth of the genus Bacillus spp ., Azotobacter spp ., Trichoderma spp , or Saccharomyces genus ( Saccharomyces spp ) At least one of the strains may be included. However, it is not limited to the microbial species, and Streptomyces sp. , Rhizobium sp. , Pseudomonas sp.) It may further include a strain.

Other beneficial microorganisms, microbial active substances and organic fertilizers may be the same as described above. Chemical fertilizers can include a variety of chemicals that can provide nitrogen, phosphorus, and potassium nutrients to support plant growth. For example, chemical fertilizers may include urea, calcium phosphate, potassium phosphate, and mixed nitrogen-phosphate-potassium (N-P-K) fertilizers. However, the present invention is not limited thereto, and the chemical fertilizer includes other materials known in the art (carbon, hydrogen, oxygen, sulfur, lime, earth, boron, iron, manganese, zinc, copper, molybdenum, chlorine, etc.). .

Hereinafter, the present invention will be described in more detail through experimental examples. These experimental examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these experimental examples.

<Experimental Example 1> Isolation of strains and selection of green blight control strains

1. Strain isolation and culture method

Gomchiw ( Ligularia fischeri ) To isolate microorganisms from the rhizome soil, 3 g of the rhizome soil was placed in 27 ml of sterile water, shaken at 28 ° C. at 180 rpm for 30 minutes, and then 1 ml was taken and suspended in 9 ml of sterile water. . To isolate microorganisms from the roots, surface sterilization of Gomchi root was crushed with a sterilized mortar, and 1 g of the root was suspended in 9 ml of sterile water. The rhizome soil and root suspension were diluted stepwise, spread on TSA (Tryptic Soybean Agar) and NA (Nutrient Agar) medium, and cultured for 48 hours at 28 ° C. Single colonies with different colors and shapes were isolated pure, 20% glycerol (glycerol), stored at -70°C, and used for experiments.

2. Separation result

The isolated strain is Cytobacillus solani ( Cytobacillus solani ) LES88 strain, and has a 16S rRNA nucleotide sequence represented by SEQ ID NO: 1.

Figure 1 shows the results of phylogenetic analysis using the 16S rRNA gene of the Cytobacillus solani ( Cytobacillus solani ) LES88 strain.

Figure 2 is a graph showing the amount (CFU / g) of bacteria isolated from the rhizosphere and the inner sphere (Endosphere) of gomchwi ( Ligularia fischeri ) cultured in TSA medium and NA medium, respectively.

The strain cultured using the NA medium in the rhizosphere is 3.7 X 10 ⁶ CFU/g, and the strain cultured using the TSA medium in the rhizosphere is 6.8 X 10 ⁶ CFU/g. The strain cultured using the NA medium in the Endosphere is 1.6 X 10 ⁴ CFU/g, and the strain cultured using the TSA medium in the Endosphere is 3.7 X 10 ⁴ CFU/g.

Among the above strains, it was confirmed that the strain having the best control effect on the blight blight was the Cytobacillus solani LES88 strain, and it was finally selected.

The LES88 strain has a 16S rRNA nucleotide sequence represented by SEQ ID NO: 1.

Table 1 is a comparison of the homology of the standard strain in close relation with the Cytobacillus solani ( Cytobacillus solani ) LES88 strain.

Name strain Accession Pairwise Similarity (%) Mismatch/Total nt Completeness (%) Cytobacillus solani FJAT-18043 LJIX01000006 99.86 2/1471 100 Cytobacillus praedii FJAT-25547 KY582959 99.79 3/1420 96.48

As shown in Table 2, the Cytobacillus solani LES88 strain of the present invention has a nucleotide sequence homology of 2/1471 with the Cytobacillus solani FJAT-18043 strain, and the similarity was found to be the most similar with 99.86%. , Cytobacillus praedii has a nucleotide sequence homology with FJAT-25547 and 3/1420, and the similarity was found to be 99.79%.

<Experimental Example 2> Antagonism test for inverse pathogens of isolated strains

Phytophthora capsici of the isolated strain ( Phytophthora capsici ) and Phytophthora drechsleri ( Phytophthora drechsleri ) The antagonistic ability was tested three times. The isolated strain was streaked at a distance of 35 mm from the center of the PDA (Potato Dextrose Agar) medium and cultured at 25° C. for 24 hours, and sterile water was used as a control. Phytophthora capsici ( P. capsici ) and Phytophthora drechsleri ( P. drechsleri ) The colonies were placed in the center of the PDA medium, respectively, and cultured at 25° C. for 5 days, and then in the inhibition zone. Microorganisms with excellent antibacterial activity were selected by measuring the size of the.

Table 2 is a table showing the antifungal activity of the LES88 strain against Phytophthora capsici , which causes root rot in various crops (including pepper plants) using a double replacement culture assay.

Treatment Mycelial growth inhibition rate (%)* Mean of mycelial growth inhibition rate (%) standard error ^1st trial ^2nd trial ^3rd trial LES88 31.1 28.1 28.7 2.0 1.23 Control** 0.0 0.0 0.0 0.0 0.0

*(Control cell cell diameter (mm) - Treatment cell cell diameter (mm)) / Control cell cell diameter (mm))X100 **Control cell is treated with sterile distilled water. The cell diameter of the control was 68.4 mm.

Table 3 is a table showing the antifungal activity of the LES88 strain against Phytophthora drechsleri , which causes root rot in various crops (including pepper plants) using a double replacement culture assay.

Treatment Mycelial growth inhibition rate (%)* Mean of mycelial growth inhibition rate (%) standard error ^1st trial ^2nd trial ^3rd trial LES88 31.1 28.1 28.7 29.3 0.77 Control** 0.0 0.0 0.0 0.0 0.0

*(Control cell cell diameter (mm) - Treatment cell cell diameter (mm)) / Control cell cell diameter (mm))X100 **Control cell is treated with sterile distilled water. The cell diameter of the control was 61.3 mm.

<Experimental Example 3> Test of the effect of controlling green blight by the treatment of the culture solution of the finally selected LES88 strain

Cytobacillus solani ( Cytobacillus solani ) In order to test the control effect on the green blight ( Ralstonia solanacearum ) of the LES88 strain, a bioassay was performed using pepper seedlings in a greenhouse.

A single colony of the cultured LES88 strain was inoculated in TSB (Tripic Soy Broth) medium and cultured at 28 ° C. at 150 rpm for 48 hours, and the concentration of the culture medium was adjusted to 1.0X10 ⁸ CFU/mL (OD ₆₀₀ =0.25).

Red pepper seeds (Super manitta, Nongwoo-bio, Korea) are washed with running tap water for 2 hours, and the surface is sterilized using 1% sodium hypochlorite and 70% ethanol to remove microorganisms on the surface, followed by a 10cm diameter pot Sowing was carried out with green soil (Seoul Bio, Korea) in it (n=15).

After 4 weeks of sowing, 50 mL of the culture solution of the isolated microorganism LES88 strain was irrigated on the red pepper seedlings with 7 to 8 true leaves, and after 7 days had elapsed, Ralstonia solanacearum , a green blight, was added to 1.0. X10 ⁸ CFU/mL (OD ₆₀₀ =0.15) was administered with 20 mL per week irrigation. After 5 weeks of treatment with the Ralstonia solana serum strain, the disease rate (%) and plant height were investigated.

Figure 3a shows the pepper seedlings tested for the green blight control effect of the culture medium of the LES88 strain on the pepper seedlings, and Figure 3b is the red pepper seedlings tested for the green blight control effect of IC (Inoculated control; pathogen inoculated control) on the pepper seedlings. tombs are shown.

In the case of red pepper seedlings of FIG. 3a treated with the culture medium of the LES88 strain for green blight control, green blight hardly occurred, whereas in the case of pepper seedlings of FIG. The distal end of the seedlings were brown, and there was no effect of controlling blight blight compared to the pepper seedlings of FIG. 3a.

Figure 4 is a culture medium of the LES88 strain in pepper seedlings, serenade (Serenade) treated or untreated (IC (Inoculated control; pathogen inoculated control) and UT (Untreated control; pathogen non-inoculated control)) of the diseased strain rate (Diseased) plant).

The serenade is a microbial pesticide, and since there is no pepper bacterial blight in the list of diseases to be controlled, serenade was treated as a control to confirm the difference in growth and control effect.

The test was performed in two replicates. In the first attempt, Serenade treatment on pepper seedlings showed an 80% morbidity rate, indicating that the control was 0% compared to the control (IC, inoculated control; 63.3% morbidity), indicating that there was no effect of controlling blight. When the pepper seedlings were treated with the culture solution of the LES88 strain, it showed a 22.2% blight disease rate and 64.9% of the control value compared to the control, indicating that it was effective.

In the second attempt, Serenade treatment on pepper seedlings showed a morbidity rate of 40%, and control (IC, inoculated control, contamination rate 100%) compared to the control was 60%, but the culture solution of the LES88 strain was When it was treated, it was found that the disease prevention rate was 20%, the control rate was 80%, and the blight control effect was higher than that of the serenade treatment.

By repeating the experiment twice, when the pepper seedlings were treated with the culture solution of the LES88 strain, an average of 21.1% of the blight disease strain rate was shown, whereas the Serenade treatment showed an average blight disease strain rate of 60.0%, The average control value by LES88 was 74.%, and the average control value by Serenade was 26.5%.

Figure 5 is in order to evaluate the effect of the culture medium of the LES88 strain on the pepper growth, the culture medium of the LES88 strain on the pepper seedlings, serenade (Serenade), or untreated (IC (Inoculated control; pathogen inoculation control) and UT) (Untreated control; non-inoculation control with pathogens)) is a table showing the plant height (Plant height).

The test was performed in two replicates. In the first attempt, when pepper seedlings were treated with the culture solution of LES88 strain, the plant height was 23.1 cm, Serenade treatment 23.6 cm, pathogen inoculated control (IC, Inoculated control) 21.5 cm, And the pathogen non-inoculated control (UT, Untreated control) showed a plant height of 22.3 cm, the difference in plant height was insignificant.

In the second attempt, when pepper seedlings were treated with the culture solution of the LES88 strain, the plant height was 33.8 cm, Serenade treatment 31.6 cm, pathogen inoculated control (IC, Inoculated control) 28.4 cm, And in the case of pathogen-uninoculated control (UT, Untreated control), it exhibited a plant height of 29.8 cm. That is, the fact that the change in plant height of red pepper seedlings caused by the LES88 strain treatment through two repeated experiments is insignificant can be interpreted that the LES88 strain treatment can effectively control the blight without the effect of inhibiting the growth of peppers.

The present invention has been looked at with a focus on preferred experimental examples, and those of ordinary skill in the art to which the present invention pertains can implement embodiments other than the detailed description of the present invention within the essential technical scope of the present invention. will be able Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Agricultural Gene Center, National Academy of Agricultural Sciences KACC81134BP 20200928

<110> REPUBLIC OF KOREA, REPUBLIC OF KOREA <120> Cytobacillus solani LES88 EXHIBITING CONTROL EFFECT AGAINST BACTERIAL WILT <130> FP-2009051-KR <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1481 <212> RNA <213> Unknown <220> <223> Cytobacillus solani LES88 <400> 1 ccccaatcat ctgtccccacc ttaggcggct ggctccttac ggttacccca ccgacttcgg 60 gtgttacaaa ctctcgtggt gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcccc 120 cgcggcatgc tgatccgcga ttactagcga ttccggcttc atgcaggcga gttgcagcct 180 gcaatccgaa ctgagaatgg ttttatggga ttggctaaac ctcgcggtct tgcagccctt 240 tgtaccatcc attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt 300 catccccacc ttcctccggt ttgtcaccgg cagtcacctt agagtgccca actgaatgct 360 ggcaactaag atcaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga 420 gctgacgaca accatgcacc acctgtcact ctgtcccccg aaggggaacg tcctatctct 480 aggattgtca gaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca 540 catgctccac cgcttgtgcg ggcccccgtc aattcctttg agtttcagcc ttgcggccgt 600 actccccagg cggagtgctt aatgcgtttg ctgcagcact aaagggcgga aaccctctaa 660 cacttagcac tcatcgttta cggcgtggac taccagggta tctaatcctg ttcgctcccc 720 acgctttcgc gcctcagtgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc 780 tccaaatctc tacgcatttc accgctacac ttggaattcc actttcctct tctgcactca 840 agtctcccag tttccaatga ccctccccgg ttgagccggg ggctttcaca tcagacttaa 900 gaaaccacct gcgcgcgctt tacgcccaat aattccggac aacgcttgcc acctacgtat 960 taccgcggct gctggcacgt agttagccgt ggctttctgg ttaggtaccg tcaaggtacc 1020 gccctattcg aacggtactt gttcttccct aacaacagag ttttacgatc cgaaaacctt 1080 catcactcac gcggcgttgc tccgtcagac tttcgtccat tgcggaagat tccctactgc 1140 tgcctcccgt aggagtctgg gccgtgtctc agtcccagtg tggccgatca ccctctcagg 1200 tcggctacgc atcgtcgcct tggtgagccg ttacctcacc aactagctaa tgcgccgcgg 1260 gcccatctgt aagtgatagc cgaagccatc tttcagcttt tctacatgag tagaaaagga 1320 ttatccggta ttagctccgg tttcccgaag ttatcccagt cttacaggca ggttgcccac 1380 gtgttactca cccgtccgcc gctaatcttt gggagcaagc tccctcagat tcgctcgact 1440 tgcatgtatt aggcacgccg ccagcgttcg tcctgagcca t 1481

Claims (10)

Cytobacillus solani for blight control ( Cytobacillus solani ) LES88 strain (KACC 81134BP). The method of claim 1,
The blight is Ralstonia solanacearum ( Ralstonia solanacearum ) generated from the pathogen, Cytobacillus solani ( Cytobacillus solani ) LES88 strain (KACC 81134BP). 3. The method of claim 2,
The strain is Cytobacillus solani containing the nucleotide sequence of SEQ ID NO: 1 ( Cytobacillus solani ) LES88 strain (KACC 81134BP). 4. The method of claim 3,
The target plant for controlling the blight is red pepper, tomato, eggplant, and solanaceae crops including tobacco Cytobacillus solani ( Cytobacillus solani ) LES88 strain (KACC 81134BP). 5. The method of claim 4,
The strain is a microbial pesticide, seed coating agent, soil improver, composting agent, foliar spreader, and Cytobacillus solani, including any one of the irrigation spraying agent, Cytobacillus solani LES88 strain (KACC 81134BP). Cytobacillus solani for green blight control ( Cytobacillus solani ) LES88 strain (KACC 81134BP), a spore of the strain, a culture solution of the strain, or a microbial preparation comprising a mixture thereof as an active ingredient. 7. The method of claim 6,
The microbial preparation is a microbial preparation comprising any one or more of a microbial pesticide, a seed coating agent, a soil improver, a composting agent, a foliar spreader, and a irrigation spraying agent. Cytobacillus solani according to any one of claims 1 to 5 selected from the steps of preparing the LES88 strain (KACC 81134BP); and
After inoculating the strain in a TSB (Tryptic Soy Broth) medium, culturing for 40-50 hours under a condition of 20-35° C. 100-200 rpm; A method of producing a microbial preparation for controlling plant blight. Claims 1 to 5, Cytobacillus solani according to any one of claims 1 to 5, comprising as an active ingredient any one or more of the cells of the LES88 strain (KACC 81134BP) or a microbial preparation prepared using the strain , Fertilizer for the control of blight in plants. 10. A method of increasing the resistance of plants to blight by irrigating the soil with the fertilizer according to claim 9.

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