KR102470864B1 - Promotes onion growth and drought tolerance by bacillus altitudinis h5-9 and uses thereof - Google Patents

Abstract

The present invention relates to a Bacillus altitudinis H5-9 strain having an effect of enhancing plant growth and reducing drying stress of plants, a microbial preparation comprising a culture solution of the strain or a mixture thereof as an active ingredient, and plant seeds using the same It relates to a method for promoting the growth of plants and a method for reducing drying stress in plants.

Description

Bacillus altitudinis H5-9 strain having onion growth promoting effect and drought tolerance enhancing effect and use thereof

The present invention relates to a Bacillus altitudinis H5-9 strain having an effect of enhancing plant growth and reducing drying stress of plants, a microbial preparation comprising a culture solution of the strain or a mixture thereof as an active ingredient, and plant seeds using the same It relates to a method for promoting the growth of plants and a method for reducing drying stress in plants.

Agricultural useful microorganisms that are currently commercialized include microbial agents that have effects such as antagonizing plant pathogenic microorganisms, solubilization of inorganic nutrients in the soil, and promotion of various plant growth, and the number of currently available chemical pesticides and fertilizers is also decreasing. Therefore, a microbial agent capable of replacing it is required.

The present invention provides a microbial preparation comprising a Bacillus altitudinis H5-9 strain, a culture solution of the strain or a mixture thereof as an active ingredient, and a method for promoting the growth of plant seeds using the same and reducing drying stress of plants. It is intended to replace existing chemical pesticides and fertilizers by providing methods.

The present invention provides a Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress in plants.

The present invention provides a Bacillus altitudinis H5-9 strain (KACC 92279P) for enhancing plant growth.

In one embodiment of the present invention, the strain may have a gyrase gene nucleotide sequence represented by SEQ ID NO: 1.

In one embodiment of the present invention, the plant may be an onion.

The present invention provides a microbial preparation comprising at least one of a Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress in plants, a culture solution of the strain, or a mixture thereof as an active ingredient.

In one embodiment of the present invention, the microbial agent may further contain at least one selected from the group consisting of plant growth regulators, plant growth stimulants, and plant nutrients.

The present invention includes at least one of a Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress of plants, a culture solution of the strain, or a mixture thereof as an active ingredient, and the microbial preparation is a plant growth regulator Provides a method for enhancing the growth of plant seeds, comprising the step of treating the plant, the area around the plant, or both with at least one of a microbial agent further comprising at least one selected from the group consisting of a plant growth stimulant and a plant nutrient. .

In one embodiment of the present invention, the plant may be an onion.

The present invention relates to the step of treating at least one of the Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress in plants and the culture solution of the strain to onions, areas around onion seedlings, or both of them. It provides a method for reducing drying stress of onions comprising

The present invention relates to at least one of microbial preparations comprising at least one of a Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress in plants, a culture solution of the strain, or a mixture thereof as an active ingredient. It provides a method for reducing drying stress of onions, comprising the step of treating onions, areas around onion seedlings, or both.

The present invention includes at least one of a Bacillus altitudinis H5-9 strain (KACC 92279P) for reducing drying stress of plants, a culture solution of the strain, or a mixture thereof as an active ingredient, and the microbial preparation is a plant growth regulator A method for reducing drying stress of onions comprising the step of treating onions, the area around onion seedlings, or both, with at least one or more of the microbial agents further comprising any one or more selected from the group consisting of plant growth stimulants and plant nutrients. to provide.

When the Bacillus altitudinis H5-9 strain according to the present invention is applied to plants, damage caused by drying stress is reduced, so other microorganisms according to the present invention are used as plant activity enhancers, plant growth regulators, plant growth stimulants and plants It can be used as a nutritional supplement.

1 is a phylogenetic diagram of the strain by analyzing the gyrase gene sequence of the Bacillus altitudinis H5-9 strain.
Figure 2a shows the growth enhancing effect of onion inoculated with control onion and Bacillus altitudinis H5-9 strain under laboratory conditions.
Figure 2b shows the drying resistance effect of onions inoculated with control onions and Bacillus altitudinis H5-9 strains under laboratory conditions.
Figure 3a shows the yield (g) of onions inoculated with control onions and Bacillus altitudinis H5-9 strains in greenhouse conditions.
Figure 3b shows the sphere diameter (mm) of the onion inoculated with the control onion and the Bacillus altitudinis H5-9 strain in a greenhouse condition.
Figure 4a is a photograph showing a control onion supplied with water in the greenhouse conditions.
Figure 4b is a photograph showing a control onion that did not supply moisture in the greenhouse conditions.
Figure 4c is a photograph showing the onion inoculated with the Bacillus altitudinis H5-9 strain in a greenhouse condition.
Figure 5 shows the yield (g) of onions inoculated with control onions and Bacillus altitudinis H5-9 strains in farm field conditions.
Figure 6a is a photograph showing the control onion in farmhouse packaging conditions.
Figure 6b is a photograph showing onions inoculated with Bacillus altitudinis H5-9 strain in farmhouse conditions.
Figure 7 shows the marketability enhancement effect of the onion inoculated with the control onion and the Bacillus altitudinis H5-9 strain in farmhouse packaging conditions.

Since the present invention can make various changes and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, the embodiments disclosed in this specification will be described in detail with reference to the drawings. The illustrative embodiments described above in the detailed description, drawings and claims are not intended to be limiting, and other embodiments than those described may be used, and other changes may be made without departing from the spirit or scope of the technology disclosed herein. are also possible

Since the description of the disclosed technology is only an embodiment for structural or functional description, the scope of rights of the disclosed technology should not be construed as being limited by the embodiment described in the text. That is, since the embodiment can be changed in various ways and can have various forms, it should be understood that the scope of rights of the disclosed technology includes equivalents capable of realizing the technical idea.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed technology belongs, unless defined otherwise. Terms defined in commonly used dictionaries should be interpreted as consistent with meanings in the context of related art, and cannot be interpreted as having ideal or excessively formal meanings unless explicitly defined in the present application.

Due to the excessive use of organic and chemical fertilizers, problems such as soil contamination, repeated crop failure, soil nutrient imbalance, and occurrence of diseases and pests occur, resulting in side effects such as reduced product quality and reduced farm household income. Nitrogen (N), an important plant nutrient, has been supplied by the use of these chemical fertilizers, but the excessive use of chemical fertilizers to maintain higher yields is a problem that goes against the principles of sustainable agriculture, which requires high productivity with low input. , there were environmental problems.

In order to solve this problem, as a means to replace chemical fertilizers, a biological method for solving safe food while minimizing the burden on the agricultural environment and ecosystem is required. Accordingly, the present invention provides a microorganism preparation comprising a strain capable of reducing drying stress of plants by the above biological method, a culture solution of the strain, or a mixture thereof as an active ingredient. In particular, the microbial preparation containing the Bacillus altitudinis H5-9 strain, the culture solution of the strain, or a mixture thereof according to an embodiment of the present invention as an active ingredient acts on plants to reduce drying stress.

As used herein, "plant" refers to any living organism belonging to the plant kingdom (i.e., any genus/species within the plant kingdom), such as, but not limited to, trees, herbs, shrubs, grasses, vines, ferns, mosses, and green algae. does not Therefore, representative plants to which microbial agents can be applied include onions, peppers, brassicas, bulbous vegetables, grains, citrus fruits, cotton, cucurbits, fruit vegetables, leafy vegetables, legumes, oilseed plants, peanuts, root vegetables, tuber vegetables, but is not limited to corm vegetables, stone fruits, tobacco, strawberries and other soft fruits, and various ornamental plants. In one embodiment, it can be applied to the onion of the present invention.

In the present specification, "culture medium" is a result obtained by inoculating a strain into a medium and culturing for a certain period of time, the culture medium itself in which the strain according to the present invention is cultured in a suitable liquid medium, and the filtrate obtained by filtering or centrifuging the culture medium to remove the strain (filtering solution or centrifuged supernatant), a cell disruption solution obtained by sonicating the culture solution or treating the culture solution with a lysozyme, and a concentrate obtained by concentrating the culture solution, filtrate, or the cell disruption solution, etc. It is not limited. In addition, the probiotic or antibacterial composition may contain a culture such as a solid medium culture, or a dried product or extract of the culture medium, but is not limited thereto, and further suitable excipients or carriers (recessive gene in a heterozygous state) possessed object) may be additionally included.

The microbial preparation of the present invention may be prepared in the form of a liquid fertilizer, and may be used in the form of a powder by adding an extender thereto, or may be formulated and granulated. However, the formulation is not particularly limited.

In the present specification, “stress” may be an abiotic stress for plants, and specifically, the abiotic stress may be drying stress.

The term "abiotic stress" is used herein in its usual sense as the adverse effect of an abiotic stressor on living organisms in a particular environment, and thus in relation to plants, an abiotic stressor on a plant in a particular environment. means the negative impact of

Biological stressors include living disturbances such as fungi or harmful insects, whereas abiotic stressors can be naturally occurring or man-made, and include temperature, dry soil, osmotic stress, drought, salt or nutrient deprivation. , all of which can cause harm to plants in the affected area.

The microbial agent of the present invention can reduce the drying stress of plants, and the plants may be onions.

The present invention provides a microbial preparation comprising a Bacillus altitudinis H5-9 strain, a culture solution of the strain or a mixture thereof as an active ingredient, and using the microbial preparation, the drying stress reduction effect of plants provide a way to provide

In the present invention, the Bacillus altitudinis H5-9 strain was deposited with the National Institute of Agricultural Sciences as of October 14, 2019, and was given KACC 92279P as the accession number.

1 is a phylogenetic diagram of the strain by analyzing the gyrase gene sequence of the Bacillus altitudinis H5-9 strain.

The nucleotide sequence of the gene is represented by SEQ ID NO: 1.

The Bacillus altitudinis H5-9 strain in the present invention may include the gene sequence represented by SEQ ID NO: 1.

The microbial preparation of the present invention is added to the above-mentioned Bacillus altitudinis ( Bacillus altitudinis ) H5-9 strain, a culture solution of the strain, or a mixture thereof, and other optional components used in the art to reduce drying stress of plants. can be included with For example, at least one selected from the group consisting of plant growth regulators, plant growth stimulants, and plant nutrients may be further included. However, it is not limited thereto.

The plant growth regulator, plant growth stimulant or plant nutrient may be a chemical or another strain of bacteria or any combination thereof. In addition, it may further contain herbicides, fungicides, pesticides, and fertilizers. However, it is not limited thereto.

The plant growth regulator may include other beneficial microorganisms, microbial activators and organic fertilizers. In addition, chemical fertilizers may be included. However, it is not limited thereto.

Other beneficial microorganisms include Bacillus spp., Azotobacter spp., Trichoderma spp . ) and/or Saccharomyces spp. Saccharomyces spp . ) may be included. However, it is not limited to the above microbial species.

The microbial active material may include enzyme precursors, microbial metabolites, organic acids, carbohydrates, enzymes, and/or trace elements. For example, microbial active substances include processed enzyme products such as yeast autolysates, humic (non-terrestrial and subsurface organic matter) materials, seaweed extracts, starch, amino acids, and zinc, iron, copper, manganese, Trace elements such as bromine and molybdenum may be included. However, it is not limited to the trace elements.

Other beneficial microorganisms, microbial activators and organic fertilizers may be the same as described above. Chemical fertilizers can include a variety of chemicals that can provide nitrogen, phosphorus, and potassium nutrients to support plant growth. For example, chemical fertilizers may include urea, calcium phosphate, potassium phosphate and mixed nitrogen-phosphate-potassium (N-P-K) fertilizers. Chemical fertilizers may also include other materials known in the art.

Other beneficial microorganisms include Bacillus spp., Azotobacter spp., Trichoderma spp . ) and/or Saccharomyces spp. Saccharomyces spp . ) may be included. However, it is not limited to the above microbial species.

The present invention is Bacillus altitudinis ( Bacillus altitudinis ) H5-9 strain, a culture medium of the strain, or a mixture thereof as an active ingredient any one or more selected from the group consisting of a microbial agent selected from the plant, the area around the plant, or both of them It provides a method for enhancing the growth of plant seeds comprising the step of treating. The plant may be an onion, but is not limited thereto.

The present invention is Bacillus altitudinis ( Bacillus altitudinis ) H5-9 strain, a culture medium of the strain, or a mixture thereof as an active ingredient any one or more selected from the group consisting of a microbial agent selected from the plant, the area around the plant, or both of them It provides a method for reducing drying stress of plants comprising the step of treating. The plant may be an onion.

Specifically, the treatment step may be performed by immersing, drenching, or spraying the Bacillus altitudinis H5-9 strain, a culture solution in which the strain is cultured, and a microbial preparation using the strain to a seed or plant. . In the case of the immersion method, the culture solution and the preparation may be inoculated by drenching the soil around the plant or by immersing the seed in the culture solution and the microbial preparation. However, it is not limited to the above inoculation method.

The method of the present invention is a method of applying a microbial preparation at any time during the life cycle of a plant, during one or more phases of the life cycle of a plant, or at regular intervals in the life cycle of a plant, or continuously throughout the life cycle of a plant. include that Therefore, microbial preparations can be applied as needed. For example, the microbial preparation may be applied to the plant during growth, before and/or during flowering, and/or before seed development and/or during seed development. In one example, the microbial preparation may be applied before, during and/or immediately after the plant is transplanted from one location to another location, such as in a greenhouse or hotbed, into the field. In another example, each microbial preparation can be applied to the plant multiple times with a desired interval period.

Regarding the soil around the plant, the surrounding area to which the present invention is applied means a range in which the microbial agent of the present invention can exert an effect, and includes both ground and underground areas. For example, it may be an area within about 2 m, within about 1 m, within about 70 cm, within about 50 cm, within about 25 cm, within about 10 cm, or within about 5 cm around a plant, plant part or fruit. However, it is not limited to the above area.

The drying stress reduction method for plants is a method for reducing the drying stress of at least one plant selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, moss, green algae, monocotyledonous plants, and dicotyledonous plants. As an embodiment, the plant may be the onion of the present invention.

The part of the plant to which the present invention is applied is not limited. Thus, it includes the entire plant as well as parts of the plant, such as seeds, shoots, stems, roots, leaves, and fruits, all of these as well as parts thereof.

In the present invention, a plant seed comprising the step of treating the plant, the area around the plant, or both of them with at least one of a microbial agent further comprising at least one selected from the group consisting of a plant growth regulator, a plant growth stimulant, and a plant nutrient. It provides a growth promotion method. The plant may be an onion.

Hereinafter, the present invention will be described in more detail through experimental examples. However, these are only for explaining the present invention in more detail and the scope of the present invention is not limited to experimental examples.

Experimental Example 1: Bacillus altitudinis ( Bacillus altitudinis ) Identification of strain H5-9

The phylogenetic location and identification of Bacillus altitudinis H5-9 strains were analyzed using gyrase gene sequencing. The nucleotide sequence similarity was analyzed with EzTaxon, and the nucleotide sequence was aligned with SINA aligner v1.2.11 to create a phylogeny, and the length of the nucleotide sequence was matched. Thereafter, a genealogy was created using a neighbor-joining algorithm with the MEGA 6.0 program, and the stability of the genealogy was confirmed by performing bootstrapping 1000 times. The nucleotide sequence of the gene is represented by SEQ ID NO: 1.

1 is a phylogenetic diagram of the strain by analyzing the gyrase gene sequence of the Bacillus altitudinis H5-9 strain.

As a result of phylogenetic analysis, strain H5-9 had 99.8% similarity with Bacillus altitudinis 41kf2b ^T (standard strain) in the gyrase gene sequence, so strain H5-9 was identified as Bacillus altitudinis. .

Experimental Example 2: Bacillus altitudinis ( Bacillus altitudinis ) Auxin production ability of strain H5-9

In order to evaluate the auxin-producing ability of the Bacillus altitudinis H5-9 strain, the H5-9 strain was cultured in TSB (Tryptic soy broth) medium at 28ºC for 24 hours, and then the Bacillus altitudinis H5-9 strain was subjected to centrifugation. Only the recovered strain was cultured for 24 hours in a medium in which 100 μg/ml of tryptophan was added to /2 TSB (Tryptic soy broth) medium. After centrifugation at 4000 rpm for 10 minutes, 40 μl of the supernatant was mixed with 80 μl of Salkowski's solution (35% perchloric acid, 1 ml of 0,5 M FeCl ₃ ). Thereafter, 2 μl of 10 mM phosphoric acid (1/40 dilution) was added and incubated at 10° C. for 24 hours, and then the auxin generating ability was measured and evaluated at an absorbance of 530 nm.

Experimental Example 3: Bacillus altitudinis ( Bacillus altitudinis ) Phosphoric acid solubilization ability of strain H5-9

In order to evaluate the phosphate solubilization ability of the Bacillus altitudinis H5-9 strain, after culturing as described above, PVK (Pikovskaya) medium (glucose 10 g, Ca ₃ (PO ₄ ) ₂ 5 g, (NH ₄ ) 2SO ₄ 0.5 g, NaCl 0.2 g, MgSO _{4.7H 2} O 0.1 g, KCl 0.2 g, yeast extract 0.5 g, MnSO ₄ .H ₂ O 0.002 g, FeSO ₄ .7H ₂ O 0.002 g, Agar 15.0 g, distilled water 1000 ml, Bromo-phenol-blue 0.025 g, pH 7.0) was cultured for 2 weeks to evaluate whether an annulus was formed.

Experimental Example 4: Bacillus altitudinis ( Bacillus altitudinis ) ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity of strain H5-9

In order to evaluate the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) deamination enzyme, the Bacillus altitudinis H5-9 strain was cultured as described above, and then ACC (30 μmol/min) was added to DF (Dworkin and Foster) minimal medium. plate) was smeared and Bacillus altitudinis H5-9 strain suspension was dropped one drop to evaluate growth.

Experimental Example 5: Bacillus altitudinis ( Bacillus altitudinis ) Seedrophore-producing ability of strain H5-9

In order to evaluate the seed pore-producing ability, the H5-9 strain was cultured as described above, and then measured and evaluated by CAS diffusion assay. 15.125 mg of chrome azurol S was dissolved in 12.5 ml of deionized water (DW) and 2.5 ml of iron (III) (1 mM FeCl ₃ 6H ₂ O, 10 mM HCl) and slowly mix an aqueous solution of 18.225 mg of HDTMA (Hexadecyl-trimethylammonium) in 10 ml of deionized water to make mixed solution A, 187.5 ml of deionized water, 3.38 g of agar, 7.56 g of Pipers, After mixing 10 mM NaOH, the pH was adjusted to 6.8 to prepare Mixed Solution B. Mixed Solution A and Mixed Solution B were mixed, and then dispensed to prepare a CAS (chrome azurol S) medium, using a sterilized scalpel. The medium was divided into two parts, one side was removed, and TSA (Tryptic soy agar) medium was dispensed to prepare the medium. The TSA side was inoculated with the H5-9 strain and cultured in an incubator at 28 ° C for 10 days, and the CAS medium turned from blue to yellow. The length of the discolored part was measured.

Table 1 below shows the experimental results of Experimental Examples 2 to 5, and is a table summarizing the physiochemical characteristics of the Bacillus altitudinis H5-9 strain.

division characteristic Characteristics related to crop growth promotion production of auxins + ACC deaminase activity + phosphoric acid solubilization + Seedropore - Morphological characteristics Cell size (diameter to width) 0.67±0.13
0.378±0.07 Colony size (3-day culture) 0.972±0.04 colony color White growth environment Growth temperature range 15℃℃ NaCl resistant 1%-10% carbon availability Casein + Starch, Lipid (Tributyrin) - Etc Catalase, Oxidase activity +

Experimental Example 6: Bacillus altitudinis in greenhouse conditions ( Bacillus altitudinis ) Onion drying tolerance enhancement effect of strain H5-9

After drenching the Bacillus altitudinis H5-9 strain under greenhouse conditions, onion seeds (variety: Quanju Junggohwang) were sown in 9cm plastic pots filled with soil to confirm the effect of onion growth promotion, and then about 2 grown for weeks. When 2 leaves appeared, 20 ml of Bacillus altitudinis H5-9 strain suspension (OD ₆₀₀ = 0.25) was drenched and cultivated for 2 months. In the case of drying stress conditions, drying stress was induced with 40% by weight of polyethylene glycol-6000 (PEG-6000) after 2 weeks of cultivation and Bacillus altitudinis H5-9 strain suspension treatment as indicated above, 4 days later, and 3 weeks After that, the live weight of the onion was measured.

Figure 2a shows the growth promoting effect of onion inoculated with control onion and Bacillus altitudinis H5-9 strain in greenhouse conditions, and Figure 2b shows onion inoculated with control onion and Bacillus altitudinis H5-9 strain in greenhouse conditions. It shows the effect of drying resistance (Con (P) is a control group applied with drying stress, Con (N) is a control group without application of drying stress).

As a result of the experiment, the live weight of the onion drenched with the Bacillus altitudinis H5-9 strain in greenhouse conditions was 3.0 g, and the live weight of the control onion was 2.45 g. The live weight of the onion drenched with the Bacillus altitudinis H5-9 strain was statistically significantly increased by about 23.1% compared to that of the control onion.

Under the condition of causing drying stress to the onion, the live weight of the onion drenched with the Bacillus altitudinis H5-9 strain was 0.5 g, and the live weight of the control onion (Con(P)) was 0.44 g. The live weight of the onion drenched with the Bacillus altitudinis H5-9 strain was statistically significantly increased by about 14.5% compared to that of the control onion.

Experimental Example 7: Bacillus altitudinis under vinyl house conditions ( Bacillus altitudinis ) Onion drying tolerance enhancement effect of strain H5-9

Onion growth was measured under natural drying conditions after drenching the H5-9 strain under greenhouse cultivation conditions. Onions (variety: Katamaru) were sown in a Styrofoam box (49.5 X 31 X 22 cm in inner diameter, 25 kg of soil) and then grown in a vinyl house. After a month of adaptation to transplantation, 45 ml of Bacillus altitudinis H5-9 strain suspension (OD ₆₀₀ = 0.5 standard 1/100) was drenched, and after that, onions were stressed under dry conditions for 4 weeks, and then onions were Yield (g) and diameter (mm) were measured.

Figure 3a shows the yield (g) of onions inoculated with control onions and Bacillus altitudinis H5-9 strains in greenhouse conditions, and Figure 3b shows control onions and Bacillus altitudinis H5-9 strains in greenhouse conditions. It shows the sphere diameter (mm) of the inoculated onion.

As a result of the experiment, the yield (g) of the onion drenched with the Bacillus altitudinis H5-9 strain in the greenhouse conditions was 4.2 g, and the yield of the control (CD; Control (Drought)) was 3.6 g. Bacillus altitudinis The yield of onions drenched with the H5-9 strain increased by about 16% compared to that of the control group.

The bulb diameter (mm) of the onion drenched with the Bacillus altitudinis H5-9 strain in the greenhouse conditions was 54 mm, and the yield of the control (CD; Control (Drought)) was 50.5 mm. Bacillus altitudinis H5-9 The yield of onion drenched with the strain was increased by about 7% compared to the yield of control onion.

Figure 4a is a photograph showing the control onion supplied with water in the greenhouse conditions, Figure 4b is a photograph showing the control onion not supplied with water in the greenhouse conditions, Figure 4c is a Bacillus altitudinis H5- in the greenhouse conditions It is a photograph showing onions inoculated with 9 strains.

Experimental Example 8: Bacillus altitudinis in open field conditions ( Bacillus altitudinis ) Onion yield and bulb diameter enhancement effect of strain H5-9

Growth was measured after perfusion treatment with Bacillus altitudinis H5-9 strain in open field conditions. After cultivating onions (variety: Katamaru) in the open field until the foliage extension period, 45 ml of Bacillus altitudinis H5-9 strain suspension (OD ₆₀₀ = 0.5 standard 1/100) was drenched every 1 week, and onion yield at harvest time and onion diameters were measured.

Figure 5 shows the yield (g) of onions inoculated with control onions and Bacillus altitudinis H5-9 strains in open field conditions.

As a result of the experiment, the yield (g) of the onion drenched with the Bacillus altitudinis H5-9 strain in open field conditions was 10.2 kg / 10a, and the yield of the control onion was 9.2 kg / 10a. The yield of onions drenched with the Bacillus altitudinis H5-9 strain increased by about 11% compared to the yield of the control onion.

The spherical diameter (mm) of the onion drenched with Bacillus altitudinis H5-9 strain in open field conditions was 1650 mm, and the spherical diameter of the control onion was 1587 mm. The bulb diameter of the onion drenched with the Bacillus altitudinis H5-9 strain was increased by about 4% compared to that of the control onion.

Figure 6a is a photograph showing a control onion in open-field packaging conditions, Figure 6b is a photograph showing onions inoculated with Bacillus altitudinis H5-9 strain in open-field packaging conditions.

According to the 'National Agricultural Products Quality Management Notice No. 2018-44 (19.1.1.)', the marketability of onions grown in open-field packaging conditions as described above is small if the diameter of the onion is less than 6cm, medium if it is 6cm to 8cm, If it is 8 cm to 9 cm, it is classified as large, and if it is more than 9 cm, it is classified as extra large.

Figure 7 shows the marketability enhancement effect of onions inoculated with control onions and Bacillus altitudinis H5-9 strains in open field conditions.

When onions were grown by inoculating Bacillus altitudinis H5-9 strains in the open field, 10% were classified as small, 85% as medium, and 5% as large. In contrast, in the case of the control group, 23% were classified as small and 77% as medium, and there were no onions classified as large. Through this, it was confirmed that when the onion was grown by inoculating the Bacillus altitudinis H5-9 strain, the marketability of the onion greatly increased.

National Academy of Agricultural Sciences KACC92279P 20191014

<110> REPUBLIC OF KOREA, REPUBLIC OF KOREA <120> PROMOTES ONION GROWTH AND DROUGHT TOLERANCE BY BACILLUS ALTITUDINIS H5-9 AND USES THEREOF <130> FP-2010063-KR <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1272 <212> DNA <213> unknown <220> <223> Bacillus altitudinis H5-9 gyrB <400> 1 actagtgatt agagggtacg gatgtgcgag ccgtcgacat cggcatcggt catgatcaca 60 attttgtgat aacgtgcttt ctctaaagtg aagtcttctc cgattccagt ccctagcgct 120 gtaatcattg aacgaacctc gttgttagat aaaattttat ctagtcgtgc tttttcaacg 180 tttaggatct tccctcttaa cggtaagatc gcttggaaat gtcgatcacg accttgctta 240 gcagatccgc ccgcagaatc tccctctacg atataaagct cagagatgga aggatcttta 300 gaagaacagt ctgccagttt cccaggcaag ctagagactt ccagtgcact tttacgtctt 360 gtcagctcac gtgccttttt ggcagccatt cttgcacgag ctgccatcac acctttctcc 420 acaattttct ttgcagcatc agggttctct aagaggaatt tctcaagtgc ttcggagaag 480 agggagtcgg taatggttct tgcttctgag ttaccgagct ttgtcttcgt ttgtccttcg 540 aattgagggt ctggatgttt gatagagata atggctgcta agccttctcg tacatcttct 600 ccgctcaaat tcgagtcacc atcttcgaat acgccatttt tacgagcata atcattgatg 660 acacgcgtca gaccggtttt aaagccagct tcgtgtgatc cgccttcata tgtgttgatg 720 ttgttggcga aagaataaat attgcttgta taggaatcgt tgtattgcag tgtaacttta 780 acggtgactc cgtctttttc accctcgatg taaactggtt cttcatgaac gacttccttc 840 gagcgattca aatgttctac atagctctta ataccgcctt catagcagta ttcgtttctt 900 cgctctttgc cttcacgtaa gtcttcaatg atgatgttga cgccttttgt taagaaagct 960 aactcacgta cacggttagc aagtgtgtcg taatcaaatt caatggtttc agtgaaaatt 1020 tctggatctg gcacaaaatg agtggttgtc cctgttacat ctgtttcacc aatgatctct 1080 aaatctccaa ctggaacacc gcgtttgaat tgttgataat gaattttccc atcacggtat 1140 acagtcacgt ctaaggtcct agataacgca ttaacaacag atgcccctac gccatgcaaa 1200 ccgccagata ctttataacc gctgccgccg aatttccac ctgcgtgcag aacggtcatg 1260 atgacttcaa tc 1272

Claims (13)

Bacillus altitudinis for reducing drying stress of onions ( Bacillus altitudinis ) H5-9 strain (KACC 92279P). According to claim 1,
The strain is a Bacillus altitudinis H5-9 strain (KACC 92279P) having a gyrase gene sequence represented by SEQ ID NO: 1. delete Bacillus altitudinis H5-9 strain (KACC 92279P) for onion growth promotion. According to claim 4,
The strain is a Bacillus altitudinis H5-9 strain (KACC 92279P) having a gyrase gene sequence represented by SEQ ID NO: 1. delete A composition for reducing onion drying stress and promoting onion growth, comprising at least one of the strain according to claim 1 or claim 4 (KACC 92279P), a culture medium of the strain, and a mixture thereof as an active ingredient. According to claim 7,
The composition further comprises at least one selected from the group consisting of an onion growth regulator, an onion growth stimulant and an onion nutrient, a composition for reducing onion drying stress and enhancing onion growth. A method for enhancing the growth of onion seeds, comprising the step of treating onions, areas around onions, or both with the composition for reducing onion drying stress and promoting onion growth according to claim 8. delete A method for reducing drying stress of onions comprising the step of treating onions, a region around onion seedlings, or both of at least one of the strain according to claim 1 or 4, a culture medium of the strain, and a mixture thereof. A method for reducing drying stress of onions comprising the step of treating onions, areas around onion seedlings, or both of them with the composition for reducing onion drying stress and promoting onion growth according to claim 7.

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