KR102000472B1 - Bacillus aryabhattai strain promoting resistance of plants against abiotic stress and use thereof - Google Patents

Abstract

The present invention relates to a novel Bacillus ariabatai strain that promotes abiotic stress resistance of a plant and a use thereof. More particularly, the present invention relates to a Bacillus aryabhattai strain H26 -2 strain (Accession No .: KACC 92165P), a composition for promoting stress resistance of a plant including the strain or a culture thereof, and a method for enhancing stress resistance of a plant using the strain.
The strain of the present invention shows the effect of increasing plant resistance to abiotic stress of plants, especially high temperature and dry environment, so that plants can grow well under environments where plant growth is insufficient due to high temperature drying and nutrition The plant production can be increased.

Description

The present invention relates to a Bacillus ariabatta strain which promotes abiotic stress resistance of a plant and a use thereof for the production of Bacillus arybhattai strain against abiotic stress and use thereof.

The present invention relates to a Bacillus ariabatai strain which promotes abiotic stress resistance of a plant and a use thereof, and particularly relates to a strain of Bacillus aryabhattai H26-2 having excellent resistance to a plant in a high temperature drying environment No. KACC 92165P), a composition for promoting stress resistance of plants using the same, a method for enhancing stress resistance of plants, and a method for promoting plant growth.

Excessive amounts of chemical fertilizers have been used in agriculture around the world to provide nutrients to support plant growth. While chemical fertilizers have provided benefits to modern agriculture, it has been observed that increased use of chemical fertilizers degrades the composition and quality of the land. Therefore, the abuse of chemical fertilizer causes soil compaction and corrosion, resulting in lowering of production rate and lowering of utilization efficiency of fertilizer.

In addition, since seed and fruit production are fundamentally limited by biological and abiotic stresses, for example, drought stress, salt stress, nutrient deficiency, heavy metal contamination, extreme temperature or immersion can cause plant growth and productivity It is an additional key factor limiting. For example, soybean (Glycine max) suffers from loss of seed germination ability during storage and is a germinable species that can not germinate when soil temperature is low, and corn and other plants are important in crop science. Improvement of abiotic stress resistance in plants is believed to be due to the fact that growing bodies are growing and growing in low temperature, drought, flood, high temperature, UV stress, ozone increase, acid rain, pollution, salt stress, heavy metals, mineralized soil, Germination will be increased and there will be an advantage in crops. In addition, biological stresses such as fungal and viral infections cause many grain losses worldwide.

As a result, fertilizer and pesticides that are being used excessively are threatening the natural environment and human health at the present time. In order to maintain the productivity and increase the income of the farm households, Interest is growing in the development of eco-friendly functional agricultural materials using microorganisms.

For example, crop cultivation methods using soil microorganisms have recently been used as one of eco-friendly agricultural techniques. Currently, cultivation of crops using soil microorganisms is widely performed in soybean plants. Microorganisms mainly used for the cultivation of these crops include (i) nitrogen fixation ability to convert gas phase nitrogen to hydrogen-bonded ammonia nitrogen (NH ₃ ) Rhizobium, which allows plants to survive; (ii) Plant growth-promoting rhizobacteria (PGPR), which promotes the growth of plants without having symbiotic relationships with host plants , Abu joss buffer rilreom spp (Azospirrilum spp.) for fixing the air nitrogen and Pseudomonas spp (Pseudomonas spp.), etc. this is typical. These plant growth promoting microorganisms are known to help plant growth by various functions such as fixation of public nitrogen, promotion of nutrient absorption of plants, and inhibition of plant disease.

In addition, studies on the use of microorganisms that produce organic acids have been continuing as one of the methods for solubilizing the insoluble phosphoric acid accumulated in the soil, one of the important plant nutrients in the soil. Studies on the phosphate-solubilizing bacteria have been conducted in Russia and Eastern Europe After the announcement of the effect of increasing the number of crops due to the development of microbial fertilizer, many studies are underway in Korea and abroad. However, solubilization of such phosphorus is mainly performed by acidification in the medium, but it is not easy to apply this method to actual soil having buffering ability for pH. Therefore, it is not only excellent in the action of producing substances promoting the growth of plants, but also by inoculating the soil with a microorganism (phosphate-solubilizing microorganism, PSM) capable of directly solubilizing the insoluble phosphoric acid immobilized on the soil, It would be beneficial to increase plant productivity and further reduce the environmental pollution as well as the economic effect on the agricultural industry.

In this way, methods to promote soil nutrition and release plant nutrients from organic matter can increase plant growth and mitigate environmental stresses to plants play a major role in increasing plant production, There is a continuing need to develop methods.

1. Korean Patent No. 10-1086367 (November 17, 2011) 2. Korean Patent No. 10-2015-0050578 (May 2015) 3. Korean Patent No. 10-2012-0075936 (2012.07.09) 4. Korean Patent No. 10-2010-0032855 (Mar. 26, 2010)

The present invention provides a novel strain effective for increasing resistance to abiotic stress of a plant and a method for enhancing resistance to abiotic stress of a plant using the strain and promoting growth and thereby increasing production .

In order to achieve the above-mentioned object, the present invention provides a strain of Bacillus aryabhattai H26-2 (accession number: KACC 92165P).

The strain according to the present invention is characterized by having 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1.

The present invention provides a composition for enhancing stress resistance of a plant comprising Bacillus aryabhattai strain H26-2 (accession number: KACC 92165P), a culture solution of the strain, or both of them as an active ingredient.

The composition for enhancing stress resistance of a plant according to the present invention is characterized by enhancing the resistance of the plant to at least one stress selected from the group consisting of moisture, temperature, salt, pH and nutrition.

The composition for enhancing stress resistance of a plant according to the present invention is characterized by enhancing the resistance of at least one plant selected from the group consisting of tree, herb, shrub, grass, vine, fern, moss, green algae, .

The composition for enhancing stress resistance of a plant according to the present invention may further include at least one selected from the group consisting of a plant growth regulator, a plant growth stimulant, and a plant nutrient.

The present invention relates to a method for producing a strain of Bacillus arybhattai strain H26-2 (Accession No. KACC 92165P), a culture of the strain, a composition comprising the strain, and a composition comprising the culture of the strain Or a pharmaceutically acceptable salt thereof, to a plant, a plant peripheral region, or both.

The method for enhancing stress resistance of a plant according to the present invention is characterized by enhancing the resistance of a plant to at least one stress selected from the group consisting of moisture, temperature, salt, pH and nutrition.

The method of enhancing the stress resistance of the plant according to the present invention is to increase the resistance of one or more plants selected from the group consisting of tree, herb, shrub, grass, vine, fern, moss, green algae, .

The present invention relates to a method for producing a strain of Bacillus arybhattai strain H26-2 (Accession No. KACC 92165P), a culture of the strain, a composition comprising the strain, and a composition comprising the culture of the strain Or more of the plant growth, plant growth, or both.

The plant growth promotion method according to the present invention is characterized in promoting the growth of any one or more plants selected from the group consisting of tree, herb, shrub, grass, vine, fern, moss, green algae, monocotyledonous plants and dicotyledonous plants .

The invention abiotic resistance of a plant to stress (resistance), especially Bacillus Ari ahbatayi new strain to increase the resistance to high-temperature drying (Bacillus aryabhattai) H26-2 strain (Accession Number: 92165P KACC), or the strain A composition for promoting stress resistance of a plant, and a method for enhancing stress resistance of a plant using the same. By using a specific strain according to the present invention, stress resistance of a plant is increased, so that plants can grow well under environment where plant growth is insufficient due to high temperature drying and nutrition, and plant growth can be promoted.

Brief Description of the Drawings Fig. 1 is a systematic analysis of a 16S rRNA base sequence of a strain according to the present invention and comparing it with other Bacillus strains.
2 is a reference scale chart for evaluating the degree of plant survival.
Fig. 3 shows the results of evaluating the degree of plant survival at high temperature drying stress.
Fig. 4 shows the results of measurement of the biomass under high temperature drying stress.
FIG. 5 is a photograph of a day after the high temperature drying stress and after the irrigation treatment.
Fig. 6 shows the results of evaluating the degree of plant survival at high temperature drying stress. The left side is spring Chinese cabbage (green light) and the right side is autumn Chinese cabbage (rose).
FIG. 7 shows the result of measurement of the biomass under high temperature drying stress. The left side is spring Chinese cabbage (green light) and the right side is autumn Chinese cabbage (rose).

In order to achieve the above object, the present invention provides a composition for promoting stress resistance of a plant including Bacillus aryabhattai strain H26-2 (accession number: KACC 92165P), the strain or a culture thereof, A method for promoting stress resistance of a plant using the composition or the like, and a method for promoting plant growth of a plant using the strain, the composition, or the like. BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the drawings.

The present invention provides a strain of Bacillus aryabhattai H26-2 (accession number: KACC 92165P). The strain may have 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1.

The present invention provides a composition for enhancing stress resistance of a plant comprising Bacillus aryabhattai strain H26-2 (accession number: KACC 92165P), a culture solution of the strain, or both of them as an active ingredient.

As used herein, the term "plant" refers to any living organism (i.e. any genus / species within the plant system) that belongs to the plant system and includes, but is not limited to, trees, herbs, shrubs, grasses, vines, ferns, . Accordingly, representative plants to which the composition can be applied include brassica, bulb vegetables, grains, citrus, cotton, gourd, fruit vegetables, leaf vegetables, leguminous plants, oilseed crops, peanuts, fennel, root vegetables, , Nuclear crops, tobacco, strawberries and other enteric plants, and a variety of ornamental plants. For example, the present invention can be applied to Chinese cabbage.

As used herein, the term " culture medium " means a culture medium obtained by culturing the strain according to the present invention in a suitable culture medium, a culture obtained by filtering or centrifuging the culture medium, A supernatant obtained by centrifuging the supernatant, a cell lysate obtained by ultrasonication of the supernatant or a lysozyme in the supernatant, a concentrate obtained by concentrating the supernatant, the filtrate, the disruptant, etc. It is not. The prophylactic or antimicrobial composition may contain a solid culture broth or a culture such as a dried product or an extract of the culture broth, but the present invention is not limited thereto and may further include a suitable excipient or carrier.

The composition of the present invention may be prepared in the form of a liquid fertilizer, and may be added in the form of powders by adding an extender, or may be granulated by formulating it into a powder. However, the formulation is not particularly limited.

The composition for enhancing stress resistance of a plant of the present invention may enhance the resistance of a plant to at least one stress selected from the group consisting of moisture, temperature, salt, pH, and nutrition.

As used herein, " stress " may be an abiotic stress on a plant, and specifically abiotic stress may be dry, hot, cold, saline, or nutrient deficient. The term "abiotic stress" is used herein in its ordinary sense as a negative effect of a non-biological factor on a living organism in a particular environment, It means impact. Biotic stresses include living inhibitors such as fungi or harmful insects, while abiotic stressors may be naturally occurring or human-made and may be characterized by temperature, dry soil, osmotic stress, drought, salt or nutrient deficiency , All of which can cause harm to the plant in the affected area. For example, the salt stress may include increased salt concentration or drought. The nutritional deficiency may be a lack of soil nutrients such as potassium, phosphate or iron in the soil area around the plant. (Soil) nutrients, such as stress resistance to phosphate deficiency, can be provided by improved solubilization of nutrients that are lacking in the soil.

The term " resistance to salt (or resistance) "is used herein in its ordinary sense to refer to the resistance of a plant to salt concentration (tolerance). Thus, "increasing the salt tolerance or resistance of a plant" typically refers to increasing the salt concentration (in its environment, such as in soil or water) when the plant is exposed to salt at a salt concentration higher than the physiologically acceptable salt concentration It means that the ability of the plant to withstand or tolerate is increased or improved.

The term " moisture resistance (or tolerance) " or " dry resistance (or tolerance) " refers to the ability of a plant to maintain the desired moisture balance and swelling even when exposed to dry conditions, Is also used herein. Thus, increasing "resistance to moisture (or dryness) " of a plant can result in better balance and expansion of the water than when plants are exposed to drought conditions that do not receive the regular amount of water required to maintain water balance and expansion To increase or improve the ability of the plant to make the plant.

The composition for promoting stress resistance of a plant of the present invention may enhance the resistance of one or more plants selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, mosses, green algae, monocotyledonous plants and dicotyledonous plants.

The present invention promotes plant growth enhancement or promotion through the enhancement of stress resistance of plants. In the above, 'plant growth' refers to the differentiation, flowering or growth of plants, germination, organs (roots, stems, leaves, fruits, etc.), and related to size and weight enhancement and yield enhancement.

The composition for enhancing the stress resistance of a plant of the present invention may be prepared by adding the above-mentioned strain of the present invention, the strain culture medium, or a mixture thereof to any other ingredient used in the art to enhance stress resistance or promote growth of plants May be further included. For example, the composition may further include at least one selected from the group consisting of a plant growth regulator, a plant growth stimulant, and a plant nutrient.

The plant growth regulator, plant growth stimulant or plant nutrient may be another strain of a chemical or a bacterium, and may be a herbicide, a bactericide, an insecticide, a fertilizer or any combination thereof.

The present invention may include other microorganisms, microbial active materials, organic fertilizers, and chemical fertilizers that are beneficial to compositions that promote plant growth.

Other beneficial microorganisms include, but are not limited to, Bacillus spp., Azotobacter spp., Trichoderma sp. spp), and Saccharomyces spp.

The microbial active material may include enzyme precursors, microbial metabolites, organic acids, carbohydrates, enzymes, and / or trace elements. For example, microbial active materials may include processed enzymatic products such as yeast self-extinguishing, humectant materials, seaweed extract, starch, amino acids, and trace elements such as zinc, iron, copper, manganese, bromine and molybdenum .

Other beneficial microorganisms, microbial active materials and organic fertilizers may be the same as described above. Chemical fertilizers may include a variety of chemicals that can provide nitrogen, phosphorus, and potassium nutrients to support plant growth. For example, chemical fertilizers may include urea, calcium phosphate, potassium phosphate and mixed nitrogen-phosphate-potassium (N-P-K) fertilizer. The chemical fertilizer may also include other materials known in the art.

The present invention relates to a method for producing a strain of Bacillus arybhattai strain H26-2 (Accession No. KACC 92165P), a culture of the strain, a composition comprising the strain, and a composition comprising the culture of the strain Or a pharmaceutically acceptable salt thereof, to a plant, a plant peripheral region, or both.

The plant parts to which the present invention is applied are not limited. Thus, it includes all of the plant as well as parts of the plant such as seeds, larvae, stems, roots, leaves, fruits, all of them, as well as some of them.

The present invention can be carried out by immersing or circulating, i.e., spraying, a culture solution obtained by culturing the strain and the strain, and a composition using the strain, in a seed or a plant. In the case of immersion, the culture medium and the preparation can be inoculated into the culture medium or composition by shaking the culture medium or the composition into the soil around the plant, and shaking.

The peripheral region to which the present invention is applied means a range in which the composition of the present invention can exert its effects and includes both the ground and the underground. For example, within about 2 m, within about 1 m, within about 70 cm, within about 50 cm, within about 25 cm, within about 10 cm, or within about 5 cm, of the plant, plant part or fruit.

The method of the present invention can be used to continuously apply the composition at any time during the plant's life cycle, during one or more stages of the life cycle of the plant, or at regular intervals in the life cycle of the plant, . Thus, the composition may be applied as needed. The composition can be applied, for example, to plants during growth, before and / or during flowering, and / or before seeding and / or during seeding. In one example, the composition may be applied before, during and / or after the plant is transplanted from one location to another, e.g., from a greenhouse or a warm field. As another example, each composition may be applied to the plant multiple times in a desired interval period.

The method of enhancing stress resistance of a plant of the present invention can enhance the resistance of the plant to at least one stress selected from the group consisting of moisture, temperature, salt, pH, and nutrition.

The method for enhancing the stress resistance of the plant of the present invention can enhance the resistance of any one or more plants selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, mosses, green algae, .

The present invention relates to a method for producing a strain of Bacillus arybhattai strain H26-2 (Accession No. KACC 92165P), a culture of the strain, a composition comprising the strain, and a composition comprising the culture of the strain Or more of the plant growth, plant growth, or both.

The plant growth promoting method of the present invention can promote the growth of any one or more plants selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, moss, green algae, monocotyledonous plants and dicotyledonous plants.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for the purpose of illustrating the present invention in more detail, but the scope of the present invention is not limited thereto.

[Example 1] Production of microorganisms and assay of plant growth promoting activity

In 2004, soil samples from Oi rice cultivation area were sampled from Oi cultivating farms in Gongju-si, Chungcheongnam-do and various strains were isolated by dilution in 1/10 Tryptic soy agar (TSA) medium.

Aminocyclopropane-1-carboxylic acid (ACC) deaminase activity was assayed for the auxin production ability, the phosphate solubilization ability and the 1-aminocyclopropane-1-carboxylic acid diamine Strain with growth promoting activity was selected. The strains used for the assay were cultured in Tryptic soy broth (TSB) at 25 ° C for 24 hours, harvested by centrifugation, and suspended in ₁₀ mM magnesium sulfate (MgSO ₄ ), followed by absorbance A ₆₀₀ = 0.2 and used uniformly as inoculum.

To evaluate auxin production, the suspension of the isolated strains was cultured in a medium containing 100 쨉 g / ml tryptic soy broth for 24 hours. Thereafter, the supernatant was centrifuged at 4,000 rpm for 10 minutes, and 40 ㎕ of the supernatant was added to a solution of Salkowski reagent (35% perchloric acid (HClO ₄ ), 0.5M ferric chloride (FeCl ₃ ) 2 ml of 10 mM phosphoric acid (H ₃ PO ₄ , 1/40 dilution) was added and cultured at 10 ° C for 24 hours. The absorbance was measured at 530 nm and evaluated.

After the inoculum was cultured as described above, 5.0 g of PVK medium (glucose 10.0 g, tricalcium phosphate (Ca ₃ (PO ₄ ) ₂ ), ammonium sulfate ((NH ₄ ) ₂ SO ₄ ) 0.5 g, sodium chloride 0.2g, magnesium sulphate _{(MgSO 4 · 7H 2 O)} 0.1g, potassium chloride (KCl) 0.2g, yeast extract 0.5g, manganese sulfate _{(MnSO 4 · H 2 O)} 0.002g, ferrous sulfate (FeSO ₄ · 7H ₂ O), 15.0 g of agar, 1000 ml of distilled water, 0.025 g of bromo-phenol-blue, pH 7.0) for 5 days.

In order to evaluate the activity of 1-aminocyclopropane-1-carboxylic acid diamine (ACCd), an enzyme involved in plant growth promotion, the strain inoculum of the present invention was cultured as described above, and then cultured in a DF minimal medium (Dworkin and Foster 1958; 1-aminocyclopropane-1-carboxylic acid (ACC) was plated (30 μmol / plate) on the culture plate of the culture supernatant, and one drop of the strain suspension was dropped to evaluate the growth.

As a result of the experiment, all of the three plant growth promoting activities measured above were selected as H26-2 strains which were found to be effective.

Total RNA was isolated from the selected H26-2 strain using a Qiagen genomnic tip kit. Polymerase chain reaction (PCR) was performed using fD1 and rP2 primers to obtain 16S The rRNA fragment was amplified and identified. The PCR conditions were repeated 35 times at 95 ° C for 4 minutes, 58 ° C for 1 minute, and 72 ° C for 2 minutes, and then kept at 72 ° C for 8 minutes. The amplified PCR fragments were analyzed for nucleotide sequence similarity using the BLAST network service at the National Center for Biotechnology Information (NCBI).

The 16S rRNA information (1,511 bp) of H26-2 obtained as a result of the experiment was described in Sequence Listing 1, and was identified as Bacillus aryavata through a systematic analysis thereof (see FIG. 1).

[Example 2] Inducing effect of resistance to high temperature drying stress of Chinese cabbage

The strain of the present invention was treated in a greenhouse condition and the cabbage was evaluated for its cabbage growth after high temperature drying stress was applied to the cabbage.

After culturing the strain of Bacillus subtilis H26-2 of the present invention in a tryptic soybean at 28 DEG C for 24 hours, a single colony was shake-cultured in 100 mL of tryptic soy broth at 28 DEG C and 150 rpm for 48 hours, (5,000 rpm, 5 minutes), the supernatant was removed, and the precipitated germinated 10 mM magnesium sulfate (MgSO ₄ ) The cells were harvested as a solution and used as a strain suspension at 10 ⁷ cells / ml.

The Chinese cabbage seeds (variety: rose) were sown on a 9 cm plastic pot containing soil, cultivated for 2 weeks in a greenhouse, treated with 20 ml of the suspension of H26-2 prepared above, After cultivation, high temperature drying stress treatment was applied. The control (Control) was treated with ₁₀ mM magnesium sulfate (MgSO ₄ ) in place of the strain suspension. The high temperature drying stress was maintained for 5 to 7 days at 35 ° C, which may affect cabbage growth, without drying. The plant survival index (PSI) was then assessed as 0 (complete death) to 5 (surviving). After the high temperature drying stress condition, water was again given to 40 ml / The survival rate (0 to 5) was evaluated (see Fig. 2 for evaluation criteria).

As a result of the experiment, the survival rate of the plant was 5 to 7 days in the high temperature drying stress condition, and the control group (control) had a plant survival rate of 0.5, whereas the strain treated group (H26-2) of the present invention was remarkably high at 1.4, Control was 1.1, and the strain treated group (H26-2) of the present invention was recovered to 3.8.

Taken together, the degree of plant survival was from 0.5 to 2.2 in the control (Control), whereas the strain treated group (H26-2) of the present invention was 1.4 to 3.8, and the strain of the present invention survived better under the drying conditions (Fig. 3).

The fresh weight of the cabbage was measured by the same method as in FIG. 2, and the water content was restored to 40 ml / port. The control group (control) ) Was 3.26 g, which was 2.65 times higher in the organism (see FIG. 4). On the other hand, the growth of the strain-treated group (H26-2) of the present invention was remarkably remarkably increased to such an extent that it could be visually recognized (see Fig. 5).

[Example 3] Tolerance effect on tolerance to high temperature drying stress according to Chinese cabbage variety

In the same greenhouse conditions as the above-described high temperature drying stress, resistance to induction of resistance was confirmed by cultivating the spring cabbage in a green light cultivar and autumn cabbage in a rose cabbage by growing and treating the strain of the present invention.

The degree of viability of the Chinese cabbage was evaluated at 0-5, and after the evaluation, it was given water at 40 ml / port for 1 day, and the degree of survival and bioavailability .

As a result, it was found that the control group (Control) was 2.47 and the strain treated group (H26-2) of the present invention was 3.67 (H26-2) of the present invention was 4.9, and the H26-2-treated group was significantly higher than that of the control group (Control) (Fig. 6, right side). In addition, when the control was restored, the control group (Control) of the Chinese cabbage was 2.65 g, and the strain treated group (H26-2) of the present invention was 3.00 g, which was somewhat higher when the strain of the present invention was treated ).

In the case of autumn Chinese cabbage (roses), the treatment and evaluation were carried out under the same conditions as those of spring cabbage. The survival rate of plants after the high temperature drying stress was 1.47 in the control group, while the strain treated group (H26-2) (H26-2) of the present invention was almost completely recovered (left side in FIG. 6), and the fresh weight of the control group (Control) was 3.13 ) Was 2.43, the strain treated group (H26-2) of the present invention had a 2.90 weight gain of 19.3% (Fig. 7, left).

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

National Institute of Agricultural Science KACC92165P 20170109

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Bacillus aryabhattai strain promoting resistance of plants          against abiotic stress and use thereof <160> 1 <170> KoPatentin 3.0 <210> 1 <211> 1406 <212> DNA <213> Unknown <220> <223> 16S rRNA of Bacillus aryabhattai H26-2 <400> 1 gaactgatta gaagcttgct tctatgacgt tagcggcgga cgggtgagta acacgtgggc 60 aacctgcctg taagactggg ataacttcgg gaaaccgaag ctaataccgg ataggatctt 120 ctccttcatg ggagatgatt gaaagatggt ttcggctatc acttacagat gggcccgcgg 180 tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag ccgacctgag 240 agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta 300 gggaatcttc cgcaatggac gaaagtctga cggagcaacg ccgcgtgagt gatgaaggct 360 ttcgggtcgt aaaactctgt tgttagggaa gaacaagtac gagagtaact gctcgtacct 420 tgacggtacc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 480 ggtggcaagc gttatccgga attattgggc gtaaagcgcg cgcaggcggt ttcttaagtc 540 tgatgtgaaa gcccacggct caaccgtgga gggtcattgg aaactgggga acttgagtgc 600 agaagagaaa agcggaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaacac 660 cgtggcgaa ggcggctttt tggtctgtaa ctgacgctga ggcgcgaaag cgtggggagc 720 aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttagagg 780 gtttccgccc tttagtgctg cagctaacgc attaagcact ccgcctgggg agtacggtcg 840 caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 900 attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctctgacaac tctagagata 960 gagcgttccc cttcggggga cagagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc 1020 gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gatcttagtt gccagcattt 1080 agttgggcac tctaaggtga ctgccggtga caaaccggag gaaggtgggg atgacgtcaa 1140 atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggatggt acaaagggct 1200 gcaagaccgc gaggtcaagc caatcccata aaaccattct cagttcggat tgtaggctgc 1260 aactcgccta catgaagctg gaatcgctag taatcgcgga tcagcatgcc gcggtgaata 1320 cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac acccgaagtc 1380 ggtggagtaa ccgtaaggag ctagcc 1406

Claims (11)

Bacillus aryabhattai strain H26-2 having 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1 and enhancing the resistance of cabbage to moisture and temperature stress which do not give water at 35 DEG C No. KACC 92165P).
delete A composition for promoting stress resistance of a Chinese cabbage to water and temperature stress which does not give water at 35 캜, comprising the strain according to any one of claims 1 to 7, the culture liquid of the strain, or both as an active ingredient.
delete delete The method of claim 3,
Wherein the composition for enhancing stress resistance of Chinese cabbage to moisture and temperature stress further comprises at least one selected from the group consisting of a plant growth regulator, a plant growth stimulant, and a plant nutrient. And a composition for promoting stress resistance of Chinese cabbage to temperature stress.
Bacillus aryabhattai strain H26-2 having 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1 and enhancing the resistance of cabbage to moisture and temperature stress which do not give water at 35 DEG C No. KACC 92165P), a culture of the strain, a composition comprising the strain, and a composition comprising the culture of the strain to a Chinese cabbage, a Chinese cabbage area, or both The method of enhancing the stress resistance of Chinese cabbage to water and temperature stress which does not give water at 35 캜. delete delete delete delete

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