KR20160128533A - Soil-microbial product containing new microorganism enterobacter sp. 04-p-1 having soluble phosphate activity and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a novel microorganism Enterobacter sp. 04-P-1 having a soluble phosphate activity, and to a soil-microbial fertilizer including the same. The Enterobacter sp. 04-P-1 (KACC 92041P) comprises nucleotide sequence of SEQ. ID NO. 1, and has the phosphate activity and salt tolerance. According to the present invention, a phosphorus fertilizer, which is easily insolubilized in soil, is solubilized in a form usable for plants as the novel microorganism Enterobacter sp. 04-P-1 and the soil-microbial fertilizer including the same are provided to a greenhouse, in which refractory phosphate is accumulated. Accordingly, the novel microorganism Enterobacter sp. 04-P-1 and the soil-microbial fertilizer reduce salts accumulated in soil and reduce damages caused by salt stress.

Description

TECHNICAL FIELD The present invention relates to a novel microorganism Enterobacter sp. 04-P-1 and a soil microorganism fertilizer containing the microorganism. 04-p-1 having soluble phosphate activity and manufacturing method thereof}

The present invention relates to a novel microorganism Enterobacter sp. 04-P-1 having a solubilizing activity for phosphoric acid and a soil microorganism fertilizer containing the same. More particularly, the present invention relates to a novel microbial enterobacteria fertilizer P-1 strain and soil microorganism fertilizer containing the microorganism Enterobacter sp. 04-P-1 strain, which reduces salt accumulated in the soil by solubilization and promotes the growth of tomato.

Phosphorus is a macronutrient that is a vital nutrient that is very important for the growth and development of plants and requires a large supply. Therefore, for the maximum harvesting of major crops in agriculture, the presence of phosphorus that is available to plants is a very important condition.

However, most of the phosphorus in the soil is insoluble and plants can not be used.

That is, phosphorus in soil exists as a chemically cohesively insoluble complex or an organically bound phytate and exists as an insoluble phosphate that plants can not use at all.

Thus, phosphorus available in natural soils is present in an amount less than the concentration of micronurti- ties. As a result, the concentration of soluble phosphorus is a limiting factor in promoting plant growth and development in the soil. Moreover, even if inorganic phosphoric acid fertilizers soluble in soil are supplied, more than 75% of them are converted into insoluble phosphate state very quickly, and plants can use only less than 25% of the supplied soluble phosphorus.

Therefore, the farmers repeated the excessive fertilizing of supplying the excessive inorganic phosphoric acid fertilizer to the farmland in order to prevent the decrease of the growth and development of the plant due to the lack of soluble phosphorus. However, most of the soluble phosphorus supplied in such excess fertilization is converted into a large amount of insoluble phosphate in the agricultural land, which accumulates and eventually becomes a salt obstacle soil in which plant growth and development are not normally performed.

In addition, these polluted soils become more and more frequent in plantation areas, which are widely used as a system for producing high value crops, which is a great problem in agriculture productivity and agricultural economy.

Fortunately, however, it is known that there are some microorganisms in the soil that can convert such insoluble phosphate into a solubilized state. These insoluble phosphate solubilizing bacteria (PSBs) maintain a kind of symbiotic relationship that allows the plants to absorb and use insoluble phosphate by converting the insoluble phosphate of the soil into soluble phosphorus by secreting organic acids or releasing specific enzymes from the rhizosphere of the plant .

PSB microorganism Pseudomonas (Pseudomonas), Bacillus (Bacillus), rayijo Away (Rhizobium), Burkholderia (Burkholderia), Achromobacter (Achromobacter), Agrobacterium (Agrobacterium), micro Cocos (Microccocus), oh Leo bakteo ( Aleobacter ), Flavobacterium ( Flavobacterium ) and some species belonging to Erwinia .

However, the insoluble phosphate-soluble spectrum of the above-mentioned strains was examined. As a result, it was found that the insoluble phosphate was soluble only for a specific insoluble phosphate or exhibited low overall activity when its solubility was wide.

Although PSB microorganisms are present in soil, they are not always high enough to dominate by competing with other microorganisms that form plant rhizome. Therefore, the amount of soluble phosphorus produced by degradation of insoluble phosphate by PSB microorganisms in soil is not high enough to increase plant growth and development. Therefore, inoculation of a large amount of PSB microorganism into the soil is useful for increasing the growth and development of the plant.

It has been reported that the insoluble phosphate solubilizing microorganisms are used to enhance plant growth.

In other words, it has been reported that Rahnella aquatilis can solubilize hydroxyapatite in an effort to discover new PSB microorganisms in addition to PSB microorganisms already known.

In addition, it has been confirmed that Enterobacter agglomerans can also decompose hydroxyapatite to generate soluble phosphorus.

On the other hand, Korean Patent Laid-Open Publication No. 2002-0017516 (a new microorganism that solubilizes phosphate fixed on volcanic rock soils) discloses Bacillus sp. Picus PSB-13 which solubilizes phosphate fixed on volcanic soils.

However, there is still a need to develop new strains that solubilize insoluble phosphate in crop growing soils.

The object of the present invention is to provide a novel microorganism Enterobacter spi 04-P-1 strain which reduces the salts accumulated in the soil and promotes the growth of tomato by solubilizing the phosphoric acid fertilizer which is easily insolubilized in the soil in a form that plants can use, To provide soil microbial fertilizers.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems.

In order to achieve the above object, the Enterobacter sp. 04-P-1 strain (KACC 92041P) of the present invention is characterized by having a base sequence represented by SEQ ID NO: 1 and having a phosphate solubilizing activity, .

In particular, the strain is characterized by solubilizing phosphoric acid fertilizer insolubilized in the soil to promote the growth of tomatoes. The strain is effective as a microbial pesticide, a seed coating agent, a soil improving agent, a composting homework, a foliar spraying agent, .

The soil microbial agent of the present invention comprises a nucleotide sequence represented by SEQ ID NO: 1 and is a novel microorganism Enterobacter sp.) 04-P-1 strain (KACC 92041P) as an active ingredient.

The method for producing a soil microorganism preparation according to the present invention comprises a step of culturing a microorganism Enterobacter ( Enterobacter sp.) Comprising a nucleotide sequence represented by SEQ ID NO: 1 and having a phosphate solubilizing activity (KACC 92041P), and inoculating the strain into a TSB medium, followed by culturing under the conditions of 20 to 35 ° C and a pH of 6 to 7.

The soil microorganism fertilizer of the present invention comprises a nucleotide sequence represented by SEQ ID NO: 1 and is a novel microorganism Enterobacter 04-P-1 strain (KACC 92041P) or the soil microorganism preparation as an active ingredient.

The present invention provides a method for enhancing the solubilization capacity of the soil in soil by treating the soil microbial fertilizer with the soil.

Alternatively, there is provided a method for promoting the growth of tomato by solubilizing insoluble phosphate in the soil by treating the soil microorganism fertilizer 3 to 4 times and then cultivating the tomato growing soil.

The present invention provides a new microbial Enterobacter sp. 04-P-1 strain and a soil microbial fertilizer containing the microbial enterobacter sp. 04-P-1 as provided in facility cultures with poorly soluble phosphate, , The amount of salts accumulated in the soil is reduced, and the damage caused by the salt obstacle is reduced.

In addition, when the strain is cultivated in the soil, the growth of tomato is promoted, and the tomato growth rate is more efficient than the existing tomato growth rate.

In addition, the reduction in phosphorus fertilizer throughput is beneficial to farmers' economic conditions.

FIG. 1 is a graph showing the effect of the Enterobacter sp.) 04-P-1 strain.
Fig. 2 is a graph showing the bacterial 16S rDNA nucleotide sequence homology and cross- linking analysis of the selected strain of Fig. 1, showing that the soluble bacterial Enterobacter sp. 04-P-1.
FIG. 3 is a graph showing the results of quantitative determination of pH-soluble solubility and pH measurement of the selected strain of FIG. 1;
FIG. 4 is a graph showing the results of the optimum growth temperature for the selected strain of FIG. 1; FIG.
FIG. 5 is a graph showing the results of the optimum growth pH for the selected strain of FIG. 1; FIG.
FIG. 6 is a graph showing the salt tolerance of the selected strain of FIG. 1; FIG.
FIG. 7 is a graph showing an effective amount of acid in the soil during the treatment of the soil of the selected strain of FIG. 1; FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail, and a detailed description of known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted.

The present invention relates to a method for isolating a bacterial strain capable of solubilizing phosphoric acid, which forms a zona pellucida, by culturing a microorganism collected from forest soil in a Pikovskaya medium supplemented with hardly soluble acid (Ca ₃ (PO ₄ ) ₂ ) As a result of the analysis of homology and flexibility, the selected strain was found to be a novel strain of Enterobacter sp.

Therefore, the present invention named this strain as Enterobacter sp. 04-P-1 strain, which was deposited with the National Institute of Agricultural Science and Technology, National Institute of Agricultural Science and Technology on Feb. 27, 2015 under accession number KACC 92041P And it was confirmed that it consists of the entire nucleotide sequence shown in SEQ ID NO: 1 or a part thereof and is composed of an open reading frame (ORF) of 1,484 bp.

The phytic acid solubilizing ability of the new strain thus constructed was quantitatively assayed and its pH was measured. As a result, the effective amount of phosphoric acid was gradually increased over the period of incubation, and the effect of solubilizing the microorganism was confirmed, and it was confirmed to increase to 597.6 ppm on the 7th day of culture .

The optimum growth range was examined by examining the physiological characteristics of the novel strain as a phosphate-solubilizing strain. The optimum growth temperature was 20 to 35 ° C, the optimum growth pH was 6 to 7, and the salt tolerance range was such that the salt (NaCl) It was confirmed that the content was 0 to 10% by weight or less based on the total composition.

In addition, the amount of phosphoric acid solubilization of the new strain in the soil was analyzed to be 761 ppm, which was 91% higher than that of the control.

In order to analyze the plant growth effect in the planting area by the above-mentioned new strain for solubilizing phosphoric acid, the new strain Enterobacter sp. 04-P-1 cultures were diluted 4-fold and treated once a week for 4 times. As a result, it was confirmed that tomato fruit yield 34.0%, fruit weight 14.8% and plant height 17.1% were increased compared with the untreated control strain .

Based on the above results, the novel strain Enterobacter sp. 04-P-1 has found that phosphate fertilizer, which is easily insolubilized in soil, is solubilized in a form that can be used by plants, thereby reducing salts accumulated in the soil and promoting the growth of tomatoes.

Since the soil microorganism preparation (culture solution) of the present invention includes the novel strain, the growth temperature and the pH of the strain exhibit the phosphate solubilizing activity of the strain under any condition, and thus the conditions are not limited. However, It can be seen that when the pH is 6 to 7 at 35 ° C, the strain activity is well established. In addition, it was confirmed that the strain had salt resistance and its salt (NaCl) content was 0 to 10% by weight or less with respect to the whole composition of the culture medium.

The soil microbial fertilizer of the present invention is characterized in that it comprises any one or more of the microbial cells of the novel strain or the soil microbial agent.

The soil microorganism fertilizer of the present invention may further contain glycerol, milk powder, milk, skim milk powder, animal or vegetable oil, etc. suitable as a microorganism protecting agent and may be added as a nutrient source such as fibrinogen powder, soybean meal powder, cottonseed powder, The present invention further includes a carbon source and nitrogen source such as bran powder, corn extract, yeast extract, molasses, glucose, sugar, corn syrup and the like and inorganic salts in an ordinary amount to further promote the efficacy of the strain.

The soil microorganism fertilizer obtained by the above method can be formulated into various forms such as powders, pellets or granules of appropriate size using a general formulator.

The microbial fertilizer of the present invention formulated in the above may be used as it is or may be dried by air drying at room temperature or by lyophilization or high temperature drying method. It may also be applied to soil after mixing with rice bran and expanding it at room temperature.

When the soil microbial fertilizer of the present invention is applied to the soil, the method of soil-related application improves the solubilization ability of the soil in the soil, more specifically, the soil microbial fertilizer is diluted 3 to 4 times, By treating the insoluble phosphate in the soil to soluble phosphate, the effect of promoting phosphoric acid uptake and growth of the tomato is maintained for a long period of time.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

< Example 1 > Phosphoric acid Solubilization  Selection of strain

1. Experimental Method

Sparingly soluble adult acid _{(Ca 3 (PO 4) 2} ) is 0.5% of Pikovskaya medium (1% Glucose, 0.05% ( NH 4) 2 SO 4, 0.01% MgSO 4 · H 2 O, 0.02% KCl, 0.001% MnSO ₄ , 0.5% Ca ₃ (PO ₄ ) ₂ , 1.8% Agar, pH 7.0) were cultivated in the forest soil and selected for solubilization of phosphoric acid to form zona pellucida.

2. Experimental results

As shown in FIG. 1, it was confirmed that one strain forming a zona pellucida was displayed on the Pikovskaya medium supplemented with 0.5% of hardly soluble acid (Ca ₃ (PO ₄ ) ₂ ) It was selected as '04-P-1 '.

< Example  2> Phosphorus Solubilization  Molecular biology of the strain

1. Experimental Method

Bacterial 16S rDNA region sequencing analysis and the relationship of bacterial type strain were analyzed with the DNAman program for the new strains selected in Example 1 above.

2. Experimental results

As a result of the above-mentioned experiment, as shown in Fig. 2, the new strain selected in Example 1 was a novel microorganism Enterobacter sp. Was identified as a strain.

As described in the deposit document attached thereto, the new strain is a new soil microorganism, Enterobacter sp . ) 04-P-1. This strain was deposited with the National Institute of Agricultural Science and Technology, National Institute of Agricultural Science and Technology, and received the accession number KACC 92041P on February 27, 2015.

< Example  3> Phosphoric acid Solubility  Quantitative assay and pH  Measure

1. Experimental Method

0.05% (NH ₄ ) ₂ SO ₄ , 0.01% MgSO ₄揃 7H ₂ O, 0.02% KCl, 0.001% MnSO ₄ ) containing 0.5% of weakly soluble phosphate (Ca ₃ (PO ₄ ) ₂ ) _4, was inoculated with _{0.5% Ca 3 (PO 4)} 2, pH 7.0) strains selected in example 1 in 100ml 1㎖ (approximately 6 × 10 ⁹ / ㎖).

Then, the inoculation medium was shaken at 28 DEG C and 150 rpm, centrifuged, and the effective phosphoric acid concentration and pH of the supernatant were measured for 7 days.

At this time, the free phosphoric acid concentration was measured by using a Phosphate Colorimetric Assay Kit (BioVision co.) For 30 minutes, and the absorbance at 650 nm was measured to calculate the effective phosphoric acid concentration.

2. Experimental results

The results of the experiment are shown in Table 1 and FIG.

Time (days) 0 One 2 3 4 5 6 7 8 Effective phosphoric acid (ppm) 0 278.3 418.5 471.4 503.4 539.3 533.2 597.6 556.4 pH 6.39 4.66 4.52 4.50 4.49 4.39 4.41 4.44 4.44

As shown in Table 1 and FIG. 3, the phosphoric acid solubilization effect of the microorganism was confirmed by increasing the effective phosphoric acid amount gradually over the incubation period, and it was confirmed that it increased to 597.6 ppm at the 7th day of cultivation.

The pH was decreased from 6.39 before the microbial treatment to 4.44 during the incubation period, and the pH was decreased as the amount of available phosphoric acid was increased.

Based on the above results, it is known that the solubilization of phosphoric acid by microorganisms is caused by the organic acid secreted by the microorganisms. In this test result, the higher the solubility of the phosphoric acid is, the more the pH is lowered so that the solubilization of phosphoric acid is caused by the organic acid.

< Example  4> Phosphorus Solubilization  Physiological characterization of the strain

1. Experimental Method

To determine the physiological characteristics of the solubilized phosphoric acid strains selected in Example 1, the optimal growth range was determined by measurement at 2 days after inoculation by culture temperature, culture pH, and salt concentration (NaCl).

At this time, each strain was cultured in R2A broth for 2 days at a temperature range of 5 ~ 45 ℃ for treatment temperature and culture conditions, and the degree of growth of the strain was measured by absorbance at 600 nm.

As the treatment pH and culture conditions, each strain was cultured at 28 ° C for 2 days in R2A broth adjusted to pH 2 to 10 with phosphate buffer, and the degree of growth of the strain was measured at an absorbance at 600 nm.

The concentration of NaCl and culture conditions were determined by incubation of each strain on R2A broth at 0 ~ 20% NaCl concentration for 2 days at 28 ℃ and the growth of the strain with absorbance at 600nm.

2. Experimental results

As shown in FIG. 4, as shown in FIG. 4, it was confirmed that the growth temperature of the new strain selected in Example 1 was a mesophilic microorganism with good growth around 20 to 35 ° C.

Also, as shown in FIG. 5, it was confirmed that the growth pH of the new strain selected in Example 1 was a good microorganism with growth around pH 6 to 7.

Also, as shown in FIG. 6, the survival rate of the new strain selected in Example 1 decreased to 10% as NaCl increased.

< Example  5> Phosphorus in the soil Solubility  Quantitative analysis

1. Experimental Method

The available phosphorus was quantitatively determined using Lancaster (Soil Chemical Analysis, 2010) method. In this case, 5 ml of the phosphoric acid solubilization strain was mixed with 100 g of the sterilized soil to which 1% of weakly soluble phosphate (Ca ₃ (PO ₄ ) ₂ ) was added and the soil was collected at 28 ° C after 8 days. 1 g of the air-dried soil was taken in a 50 ml tube, 4 ml of the extract was added, and the mixture was shaken for 10 minutes and filtered through a No. 2 filter.

The color development was carried out by reference to molybdenum method (Soil Chemical Analysis, 2010) by 1-amino-2-naphthol-4-sulfonic acid. Ammonium molybdate diluted sulfuric acid mixture solution (working solution) and a coloring solution were added, respectively. The mixture was developed at 30 ° C for 30 minutes, and absorbance was measured at a wavelength of 720 nm.

2. Experimental results

As a result of the above-mentioned experiment, as shown in FIG. 7, the Enterobacter sp. In the soil treated with the strain 04-P-1, the effective amount of phosphorus was measured 76 days after the treatment, and it was increased by 91% compared to the control.

< Example  6> Phosphoric acid solubilization  Analysis of Plant Growth Effect in Microbiological Facilities

1. Experimental Method

The fresh strain culture of Example 1 above was treated with tomato seedlings which were placed using a three-round recuperative method for 20 weeks in a tomato plantation field containing an insoluble phosphate.

At this time, 50 ml of the strain cultured in TSB medium (1.7% tryptone, 0.3% soy peptone, 0.5% sodium chloride, 0.25% dipotassium hydrogen phosphate, 0.25% dextrose) for 17 hours at 28 ° C was diluted 4 times 4 times. After 10 ~ 12 weeks of the first inoculation, tomato yield, fruit weight and fruit yield were measured.

2. Experimental results

The results of the experiment are shown in Table 2 below.

Treatment Number of fruit () increase(%) Overweight (g) increase(%) Length (cm) increase(%) Control 6.6 0 101.9 0 149.6 0 The new strain Enterobacter sp. 04-P-1 8.9 34.0 117.0 14.8 175.3 17.1

As shown in Table 2, the phosphate-soluble microbes Enterobacter isolated in Example 1 were added to the soil of the cultivation facility where the insoluble phosphate was accumulated, sp. 04-P-1 cultures were diluted 4 times and treated 4 times a week for 1 week. As a result, the yields of tomato fruit 34.0%, 14.8% and 17.1% were increased.

As shown in the above results, the novel microorganism showing superior ability for solubilizing the insoluble phosphate by the organic acid to the soil microbial secretion in the present invention Enterobacter sp (Enterobacter sp.) 04-P-1, it is possible to cultivate more efficient tomato crops, so that it can be used as an effective ingredient of fertilizer for soil improvement.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined by the appended claims. It will be possible. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Depositor Name: National Institute of Agricultural Science

Accession number: KACC 92041P

Funding date: February 27, 2015

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Soil-microbial product containing new microorganism enterobacter          sp. 04-p-1 having soluble phosphate activity and manufacturing          method thereof <130> P2015-0028 <160> 1 <170> Kopatentin 2.0 <210> 1 <211> 1484 <212> DNA <213> Enterobacter sp. 04-P-1 <400> 1 ttagagtttg ttcctggctc agattgaacg ctggcggcag gcctaacaca tgcaagtcga 60 gcggtagcac agagagcttg ctctcgggtg acgagcggcg gacgggtgag taatgtctgg 120 gaaactgcct gataggaggg gataactact ggaaacggta gctaataccg cataacgtcg 180 caagaccaaa gagggggacc ttcgggcctc ttgccatcag atgtgcccag atgggattag 240 ctagtaggtg gggtaatggc tcacctaggc gacgatccct agctggtctg agaggatgac 300 cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag tggggaatat 360 tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt atgaagaagg ccttcgggtt 420 gtaaagtact ttcagcgagg aggaaggcat tgaggttaat aacctcagtg attgacgtta 480 ctcgcagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg gagggtgcaa 540 gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg gtctgtcaag tcggatgtga 600 aatccccggg ctcaacctgg gaactgcatt cgaaactggc aggctagagt cttgtagagg 660 ggggtagaat tccaggtgta gcggtgaaat gcgtagagat ctggaggaat accggtggcg 720 aaggcggccc cctggacaaa gactgacgct caggtgcgaa agcgtgggga gcaaacagga 780 ttagataccc tggtagtcca cgccgtaaac gatgtcgact tggaggttgt tcccttgagg 840 agtggcttcc ggagctaacg cgttaagtcg accgcctggg gagtacggcc gcaaggttaa 900 aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgatgc 960 aacgcgaaga accttaccta ctcttgacat ccacggaatt tagcagagat gctttagtgc 1020 cttcgggaac cgtgagacag gtgctgcatg gctgtcgtca gctcgtgttg tgaaatgttg 1080 ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg ccagcggttc ggccgggaac 1140 tcaaaggaga ctgccagtga taaactggag gaaggtgggg atgacgtcaa gtcatcatgg 1200 cccttacgag tagggctaca cacgtgctac aatggcgtat acaaagagaa gcgacctcgc 1260 gagagcaagc ggacctcata aagtacgtcg tagtccggat tggagtctgc aactcgactc 1320 catgaagtcg gaatcgctag taatcgtaga tcagaatgct acggtgaata cgttcccggg 1380 ccttgtacac accgcccgtc acaccatggg agtgggttgc aaaagaagta ggtagcttaa 1440 ccttcgggag ggcgcttacc actttgtgat tcatgactgg ggtg 1484

Claims (10)

1 &lt; / RTI &gt;
New microbial Enterobacter sp.) 04-P-1 strain (KACC 92041P).
The method according to claim 1,
Wherein the strain has a phosphate solubilizing activity.
New microbial Enterobacter sp.) 04-P-1 strain (KACC 92041P).
The method according to claim 1,
The above-mentioned strains are used for solubilizing phosphate fertilizer insolubilized in soil to promote the growth of tomatoes,
New microbial Enterobacter sp.) 04-P-1 strain (KACC 92041P).
The method according to claim 1,
Wherein said strain is salt tolerant.
New microbial Enterobacter sp.) 04-P-1 strain (KACC 92041P).
The method according to claim 1,
A microbial pesticide, a seed coating agent, a soil improving agent, a compostant homework agent, a foliar spraying agent, or a root spraying agent,
New microbial Enterobacter sp.) 04-P-1 strain (KACC 92041P).
A novel microorganism according to claim 1, which is Enterobacter sp.) 04-P-1 strain (KACC 92041P)
Soil microorganism preparation.
A novel microorganism according to claim 1, which is Enterobacter sp.) 04-P-1 strain (KACC 92041P)
Culturing the strain at a temperature of 20 to 35 DEG C and a pH of 6 to 7 after inoculating the strain into a TSB medium;
A method for producing a soil microorganism preparation.
A soil microbial fertilizer comprising at least one of the microorganism Enterobacter sp. 04-P-1 strain (KACC 92041P) according to claim 1 or the soil microorganism preparation according to claim 6 as an active ingredient. .
A method for enhancing the solubilization ability of the soil in the soil by treating the soil microbial fertilizer according to claim 8 with the soil.
A method for promoting the growth of tomato by solubilizing the insoluble phosphate in the soil by treating the soil microorganism preparation according to claim 6 with 3 to 4 times of diluting and cultivating the tomato growing soil.

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