CN112500222A - Melon growth-promoting enzyme liquid fertilizer and application thereof - Google Patents

Melon growth-promoting enzyme liquid fertilizer and application thereof Download PDF

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CN112500222A
CN112500222A CN202011306100.9A CN202011306100A CN112500222A CN 112500222 A CN112500222 A CN 112500222A CN 202011306100 A CN202011306100 A CN 202011306100A CN 112500222 A CN112500222 A CN 112500222A
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melon
liquid fertilizer
soil
enzyme liquid
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康利允
赵卫星
李晓慧
马政华
高宁宁
常高正
梁慎
徐小利
李海伦
王慧颖
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INSTITUTE OF HORTICULTURE HENAN ACADEMY OF AGRICULTURAL SCIENCES
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
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  • Fertilizers (AREA)

Abstract

The invention discloses a melon growth-promoting enzyme liquid fertilizer and application thereof. Aims to overcome the continuous cropping obstacles of the muskmelon in the greenhouse and simultaneously realize the harmless digestion and utilization of agricultural resource wastes. The enzyme liquid fertilizer can improve the utilization rate of soil nutrients, improve the physical and chemical properties of soil, comprehensively improve the soil, make the soil soft, breathable, water-retaining, fertilizer-retaining, drought-resisting, waterlogging-resisting and improve the ground temperature; the beneficial microorganisms contained in the ferment liquid fertilizer can kill pathogenic bacteria in soil, overcome continuous cropping diseases of crops, control the occurrence of plant diseases and insect pests, stably increase the yield and improve the fruit quality; the waste melon peels and melon seedlings are prepared into the ferment liquid fertilizer, so that agricultural resource wastes can be fully cleared up, the sustainable development of agricultural production is facilitated, and the ecological environment protection of farmlands is also facilitated.

Description

Melon growth-promoting enzyme liquid fertilizer and application thereof
Technical Field
The invention relates to the technical field of melon cultivation, and particularly relates to a melon growth-promoting enzyme liquid fertilizer and application thereof.
Background
The melon is one of the favorite fruits, and has high nutritive value and economic value. However, because greenhouse melons can be planted in different seasons, ecological balance in a natural state is broken, and the phenomena of poor plant growth, reduction of yield and quality, aggravation of soil-borne diseases and insect pests and the like are caused; therefore, the continuous cropping obstacle of the melons becomes one of the bottlenecks limiting the sustainable development of the melon industry. The data show that the yield reduction of the melons caused by continuous cropping obstacles of the melons is up to 70% in some places, and even more, the melons have no grain harvest and are serious to limit the planting of the melons. The main causes of continuous cropping obstacle of melon are:
(1) the reason of the physical and chemical properties of soil is as follows: the long-term continuous cropping of crops can destroy the granular structure of soil, which causes salinization of the soil, poor air permeability and deterioration of physical properties. Different crops have respective specific nutrient absorption rules, after the same crop is continuously cultivated for a long time, specific nutrients in soil are absorbed to cause deficiency of the elements, and the elements with smaller absorption amount are enriched to cause serious imbalance of the nutrients in the soil, so that soil salinity is continuously accumulated; the soil salinization causes the reduction of the soil solution permeability of the crop rhizosphere, the crop root system cannot absorb water and nutrients in the soil, the activity of the soil enzyme is reduced, the effectiveness of the soil nutrients is reduced, and meanwhile, the living environment of soil microorganisms is influenced, so that the stress resistance of the crop is reduced;
(2) the soil biological environmental reason is as follows: continuous cropping changes the micro-ecological environment of soil, reduces the population, quantity and activity of beneficial microorganisms in the soil, enriches pathogenic microorganisms, breaks the micro-ecological balance of the soil and causes frequent occurrence of soil-borne diseases.
Under the condition, agricultural science and technology personnel carry out experimental research from different angles and also obtain a plurality of achievements. For example, Zhufang hong, a method for overcoming continuous cropping obstacles of melons in a continuous cropping greenhouse (CN 201910380758.5), is used for physically disinfecting soil by combining a high-temperature greenhouse closing technology of mulching film secondary coverage after the melons are harvested in 7-8 months in summer, but has certain limitation because the soil is not fundamentally conditioned.
On the other hand, the planting area of the melons in China is about 370 kilo hectares, and residues such as the melons with non-commercial values and the trimmed fresh plant bodies left in the melon field every year are not fully utilized; but a large amount of germs and insect pests carried by the melon field are left in the melon field, which are easy to induce next-crop melon regeneration diseases and cause ecological environment pollution of soil, water body and the like; therefore, how to effectively utilize the agricultural resource wastes, realize carbon sequestration and returning to the field, avoid environmental pollution, and promote the sustainable development of agriculture is an urgent issue to be solved.
Disclosure of Invention
The invention aims to solve the technical problem of providing a ferment liquid fertilizer which is applied to greenhouse melons and aims to overcome the continuous cropping obstacles of greenhouse melons and simultaneously realize harmless digestion and utilization of agricultural resource wastes.
In order to solve the technical problems, the invention adopts the following technical scheme:
a melon growth-promoting enzyme liquid fertilizer is developed and prepared by the following steps:
(1) chopping the waste melon peel and melon seedlings for later use;
(2) taking 8-12 parts by weight of brown sugar, 0.8-1.2 parts by weight of microbial agent and 80-120 parts by weight of water, uniformly mixing, and fermenting for 2-10 hours to obtain zymocyte liquid;
(3) taking 15-20 parts of the chopped melon peel and 15-20 parts of melon seedlings in the step (1), mixing the cut melon peel and the cut melon seedlings with 5-12 parts of bean cakes, 5-10 parts of bean curd residues, 5-10 parts of bran, 5-10 parts of rice hulls, 0.8-1.2 parts of potassium humate and 0.08-0.12 part of polyglutamic acid, putting the mixture into the zymocyte liquid, uniformly stirring, fermenting at normal temperature for 2-6 months, and filtering out broken slag to obtain a melon ferment stock solution;
(4) taking 12-18 parts of urea, 6-9 parts of monopotassium phosphate, 5-7 parts of calcium superphosphate, 12-18 parts of potassium nitrate, 5-7 parts of ammonium nitrate, 0.8-1.2 parts of borax, 0.8-1.2 parts of zinc sulfate, 0.8-1.2 parts of manganese sulfate, 0.8-1.2 parts of magnesium sulfate, 6-10 parts of potassium humate, 0.8-1.2 parts of polyglutamic acid, 80-120 parts of water and 45-55 parts of melon ferment stock solution, stirring until the fertilizer is completely dissolved, and continuing to ferment at normal temperature for 1-3 days to obtain the ferment liquid fertilizer.
Researches show that in the fermentation process, various enzymes and active substances secreted by the metabolism of enzyme bacteria, such as saccharifying enzyme, protease, lipolytic enzyme, cellulolytic enzyme, oxidoreductase, alcoholic enzyme, urease and the like, have strong aerobic fermentation decomposition capability, and various enzymes generated by the fermentation process have strong catalytic capability, can decompose chemical substances such as pesticides and the like, dissolve solidified nutrient components such as phosphorus, potassium, trace elements and the like in soil, improve the content of effective nutrients in the soil and reduce the harm of continuous cropping obstacles to crops.
In the step (2), the microbial agent is prepared by mixing a rhodopseudomonas palustris agent, a bacillus subtilis agent and a lactic acid bacteria agent according to a volume ratio of 3-6: 0.8-1.2, wherein the rhodopseudomonas palustris agent contains 1.8-2.2 hundred million/ml effective viable bacteria, the bacillus subtilis agent contains 1800-2200 million/g effective viable bacteria, and the lactic acid bacteria agent contains 18-22 hundred million/g effective viable bacteria.
The rhodopseudomonas palustris is a photosynthetic bacterium, has the protein content of 65 percent, is rich in various vitamins, coenzymes and other bioactive substances and trace elements, has strong adaptability, has strong decomposition and conversion capability, has certain tolerance and decomposition capability on phenol and other substances, and the like; the bacillus subtilis can compete with pathogenic bacteria for nutrition around plants by successfully colonizing to roots, body surfaces or bodies of the plants, secretes antibacterial substances to inhibit the growth of the pathogenic bacteria, induces a plant defense system to resist the invasion of the pathogenic bacteria, and can inhibit various plant diseases caused by plant pathogenic bacteria such as filamentous fungi; the lactobacillus has strong bactericidal capacity, can effectively inhibit the activity of harmful microorganisms and the rapid putrefaction and decomposition of organic matters, can decompose lignin and cellulose which are not easily decomposed in a normal state, and ferment and decompose the organic matters, and can inhibit the proliferation of pathogenic bacteria generated by continuous cropping obstacles; the three types of microbial bacteria supplement each other, can be quickly adapted to the soil environment and become dominant floras, and further improve the soil environment of the root.
In the step (3), stirring for 1-2 times every day in the first month of starting fermentation; and (4) continuing to ferment until no fermentation gas is generated, sealing the fermentation container, placing the fermentation container in a shade place, continuing to ferment for 2-5 months, and filtering out the crushed slag to obtain the fermented soybean milk.
After melon seedlings are planted in a greenhouse, 350-450 ml/plant of ferment liquid fertilizer diluent is respectively irrigated to seedlings on 0 th day, 20 th day, 60 th day and 80 th day, and the ferment liquid fertilizer diluent is prepared by mixing the ferment liquid fertilizer and water according to the proportion of 1: 100-800 volume ratio. Other management measures are the same as the conventional measures.
A greenhouse melon cultivation fertilizing method comprises the steps of spraying 800-1200 times of enzyme liquid fertilizer diluent to melon seedling leaves every 15 days after melon seedlings are planted for 1 month, spraying 280-320 ml/plant for the first time, spraying 380-420 ml/plant for the second time, spraying 450-550 ml/plant for the third time, and spraying 450-550 ml/plant for the fourth time. Other management measures are the same as the conventional measures.
Compared with the prior art, the invention has the main beneficial technical effects that:
1. the ferment liquid fertilizer contains rich active enzymes, has strong decomposition and transformation capability, and can convert nutrients and macromolecular organic components fixed by soil into available nutrients, thereby improving the utilization rate of soil nutrients, improving the physical and chemical properties of the soil, comprehensively improving the soil, ensuring that the soil is soft, breathable, water-retaining, fertilizer-retaining, drought-resistant, waterlogging-resistant and ground temperature-increasing.
2. Beneficial microorganisms contained in the ferment liquid fertilizer can be quickly adapted to the soil environment, and can be proliferated into dominant flora in the soil to inhibit or kill pathogenic bacteria in the soil, so that continuous cropping diseases of crops are overcome, the occurrence of plant diseases and insect pests is controlled, the yield can be stably increased, the fruit quality is improved, and the ferment liquid fertilizer has great significance for the development of modern agriculture.
3. The waste melon peels and melon seedlings are prepared into the ferment liquid fertilizer, so that agricultural resource wastes (more than 300 ten thousand tons of ferment liquid fertilizer can be prepared if the national waste melon peels and melon seedlings are completely utilized), the sustainable development of agricultural production is facilitated, and the ecological environment protection of farmlands is also facilitated.
Detailed Description
The following examples are given to illustrate specific embodiments of the present invention, but are not intended to limit the scope of the present invention in any way.
The instruments and devices referred to in the following examples are conventional instruments and devices unless otherwise specified; the raw materials are all conventional products on the market if not specifically indicated; the detection methods involved are conventional methods unless otherwise specified.
Example 1: the melon growth-promoting enzyme liquid fertilizer is prepared by the following steps:
(1) chopping the waste melon peel and melon seedlings for later use;
(2) according to the weight ratio, 10 parts of brown sugar, 1 part of microbial agent and 100 parts of water are uniformly mixed and then fermented for 2-10 hours to obtain a zymophyte liquid; the microbial agent is prepared by mixing a rhodopseudomonas palustris agent, a bacillus subtilis agent and a lactic acid bacteria agent according to a volume ratio of 4:1:1, wherein the rhodopseudomonas palustris agent (produced by Changsha Eggery biotechnology limited) contains 2 hundred million/ml effective viable bacteria, the bacillus subtilis agent (produced by Wuhan Keno biotechnology limited) contains 2000 hundred million/g effective viable bacteria, and the lactic acid bacteria agent (produced by Jiangxianyan ocean biotechnology limited) contains 20 hundred million/g effective viable bacteria.
(3) Mixing 18 parts of the chopped melon peel and 1 part of melon seedlings in the step (1) with 8 parts of bean cakes, 7 parts of bean curd residues, 8 parts of bran, 8 parts of rice hulls, 1 part of potassium humate and 1 part of polyglutamic acid, putting into the fermentation bacteria liquid, uniformly stirring, and fermenting at normal temperature; stirring for 2 times a day in the first month of starting fermentation; and (4) continuing to ferment until no fermentation gas is generated, sealing the fermentation container, placing the fermentation container in a shade place, continuing to ferment for 5 months, and filtering to remove the crushed slag.
(4) Taking 15 parts of urea, 8 parts of monopotassium phosphate, 6 parts of calcium superphosphate, 15 parts of potassium nitrate, 6 parts of ammonium nitrate, 1 part of borax, 1 part of zinc sulfate, 1 part of manganese sulfate, 1 part of magnesium sulfate, 8 parts of potassium humate, 1 part of polyglutamic acid, 100 parts of water and 50 parts of melon enzyme stock solution, stirring until the fertilizer is completely dissolved, and continuing to ferment at normal temperature for 3 days to obtain the enzyme liquid fertilizer.
Example 2: greenhouse melon growth-promoting cultivation fertilization verification test
(1) And (3) experimental design:
the field test is carried out in a modern test base (Yuanyang) multi-span shed of agricultural academy of sciences in Henan province in 3-7 months in 2020; seedling raising is carried out 3-19 days in 2020, field planting is carried out 8 days in 4 months, harvesting is carried out 7 days in 7 months, and other management measures are carried out according to the conventional method; the muskmelon Xutong No. 8 is supplied for the tested variety Henan province, agricultural academy of sciences and gardening research institute.
The tested soil has been planted with muskmelons from 2009 to today, two crops in one year, a certain degree of continuous cropping obstacles exist, and the physical and chemical properties of the soil before sowing: organic matter (oil bath heating potassium dichromate oxidation-volumetric method) 13.8 g ∙ kg-1pH (measured by potentiometry) of 8.4, total nitrogen (Kjeldahl method) of 1.06 g ∙ kg-166.9 mg ∙ kg of alkaline hydrolysis nitrogen (alkaline hydrolysis diffusion method)-1164 mg ∙ kg of available phosphorus (sodium bicarbonate leach-molybdenum antimony colorimetric method)-1448 mg ∙ kg of quick-acting potassium (ammonium acetate extraction-flame photometer method)-1。
400ml of ferment liquid fertilizer diluent is poured on each plant at 0, 20, 60 and 80 days after the permanent planting of the melon seedlings, and the diluent is formed by diluting the ferment liquid fertilizer and clean water in the volume ratio of 1:100, 1:200, 1:400 and 1:800 respectively.
Collecting soil samples at the initial stage of the result and the harvest stage respectively, and measuring the activity of the soil enzyme, wherein the urease adopts a starch blue colorimetric method, the invertase adopts a 3, 5-dinitrosalicylic acid colorimetric method, and the alkaline phosphatase adopts a disodium phenyl phosphate colorimetric method. Collecting soil samples in the harvest period of muskmelons, measuring the quantity of microorganisms which can be cultured in soil organic matters, total nitrogen, alkaline hydrolysis nitrogen, quick-acting phosphorus, quick-acting potassium and rhizosphere soil, measuring the quantity of the microorganisms by adopting a dilution plate counting method, culturing, separating and counting various microorganism groups of bacteria, fungi and actinomycetes by respectively adopting a beef extract peptone culture medium, a mart culture medium and an improved Gao's I culture medium, measuring the yield after the muskmelons are harvested, and measuring the sugar content of the center and the edge by using a handheld sugar measuring instrument.
(2) As a result:
the enzyme liquid fertilizer can increase the yield and the quality of the melons (the results are shown in table 1), and compared with the enzyme liquid fertilizer treatment, the enzyme liquid fertilizer treatment yield and the sugar degree are both obviously increased, the yield is increased by 5.3-21.5%, and the overall yield is increased by about 11%; the amplification of the heart sugar is 5.7 to 13.6 percent, and the total increase is about 9 percent; the increase of the edge sugar is 12.3 to 24.9 percent, and the total increase is 18.3 percent.
TABLE 1 yield and sugar content of muskmelon processed
Figure 651791DEST_PATH_IMAGE001
Note: different lower case letters after the same column of data represent significant differences at the 0.05 level; the same applies below.
The enzyme liquid fertilizer can increase the activities of urease, invertase and alkaline phosphatase in soil at the initial fruit stage and the harvest stage of the muskmelon (see table 2), compared with the treatment without the enzyme liquid fertilizer, the activities of the urease in the soil at the initial fruit stage and the harvest stage of the muskmelon are obviously increased, the activity increases of the urease are respectively 16.6-129.8% and 20.7-89.8%, and the activity increases by 67.2% and 55.3% on the whole; the activity of the invertase is respectively increased by 28.3-77.9% and 20.0-63.0%, and is increased by 55.8% and 43.5% on the whole; the alkaline phosphatase enzyme amplification is 24.5% -65.8% and 14.2% -42.0%, respectively, and the overall increase is 43.0% and 26.7%.
TABLE 2 soil enzyme Activity at early fruiting and harvest stages of melon under various treatment conditions
Figure DEST_PATH_IMAGE002
。
The enzyme liquid fertilizer can increase the soil nutrient content of the muskmelon in the harvest period (see table 3), and compared with the treatment without the enzyme liquid fertilizer, the soil nutrient content of the muskmelon in the harvest period is obviously increased except organic matters, the organic matter amplification is 1.50-3.29%, and the total increase is about 2.36%; the increase of the alkaline hydrolysis nitrogen is 12.2 to 26.8 percent, and the increase is 20.3 percent on the whole; the effective phosphorus amplification is 5.68-10.8%, and is increased by 8.13% on the whole; the quick-acting potassium has an amplification degree of 4.74-9.7%, and the total increase is 7.07%.
TABLE 3 soil nutrient content at harvest stage of melon under various treatment conditions
Figure 959144DEST_PATH_IMAGE003
。
The enzyme liquid fertilizer can increase the number of rhizosphere soil microorganisms in the melon harvest period (see table 4), and compared with the enzyme liquid fertilizer treatment, the enzyme liquid fertilizer treatment obviously increases the number of rhizosphere soil microorganisms in the melon harvest period, the bacterial amplification is 53.3% -139.0%, and the total increase is 104.4%; the fungal amplification is 39.1% -153.3%, and is increased by 108.2% on the whole; the actinomycete amplification is 30.9-140.4%, and the total increase is 97.5%.
TABLE 4 microbial count (CFU/g) of rhizosphere soil in harvest stage of melon under each treatment condition
Figure DEST_PATH_IMAGE004
。
Example 3: greenhouse muskmelon continuous cropping cultivation fertilization verification test
(1) The implementation mode is as follows: the method is carried out in a modern test base (Yuanyang) multi-span shed of agricultural academy of sciences in Henan province in 3-7 months in 2020. And 3, 19 days of seedling culture in 2020, 8 days of field planting in 4 months, 7 days of harvesting in 7 months, and performing other management measures according to the conventional method. The variety to be tested is muskmelon Xutong No. 8 bred by horticulture research institute of academy of agricultural sciences in Henan province.
Muskmelons are planted in tested soil from 2009 to the present, and the soil is planted in two crops in one year before sowing, wherein the physical and chemical properties of the soil before sowing are as follows: organic matter 13.8 g ∙ kg-1pH 8.4, total nitrogen 1.06 g ∙ kg-166.9 mg ∙ kg of alkaline hydrolysis nitrogen-1164 mg ∙ kg of available phosphorus-1448 mg of rapid-acting potassium ∙ kg-1。
After the muskmelon grows into a rapid growth period (about 1 month for permanent planting), spraying enzyme liquid fertilizer diluted 1000 times by clear water to leaves of the muskmelon every 15 days, wherein the volume of the enzyme liquid fertilizer is 300ml for each muskmelon in the first time, 400ml for each muskmelon in the second time, 500ml for each muskmelon in the third time and 500ml for each muskmelon in the fourth time.
(2) As a result: the melon ferment liquid fertilizer can increase the yield and the quality of melons, the yield is increased by 10.5%, the heart sugar is increased by 8.7%, and the side sugar is increased by 14.4%.
The present invention is described in detail with reference to the examples above; however, those skilled in the art will understand that various changes may be made in the specific parameters of the embodiments described above, or equivalent substitutions of related method steps and materials may be made without departing from the spirit of the invention, thereby forming a plurality of specific embodiments, which are common variations of the invention and will not be described in detail herein.

Claims (5)

1. The melon growth-promoting enzyme liquid fertilizer is characterized by being prepared through the following steps:
(1) chopping the waste melon peel and melon seedlings for later use;
(2) taking 8-12 parts by weight of brown sugar, 0.8-1.2 parts by weight of microbial agent and 80-120 parts by weight of water, uniformly mixing, and fermenting for 2-10 hours to obtain zymocyte liquid;
(3) taking 15-20 parts of the chopped melon peel and 15-20 parts of melon seedlings in the step (1), mixing the cut melon peel and the cut melon seedlings with 5-10 parts of bean cakes, 5-10 parts of bean curd residues, 5-10 parts of bran, 5-10 parts of rice hulls, 0.8-1.2 parts of potassium humate and 0.08-0.12 part of polyglutamic acid, putting the mixture into the zymocyte liquid, uniformly stirring, fermenting at normal temperature for 2-6 months, and filtering out broken slag to obtain a melon ferment stock solution;
(4) taking 12-18 parts of urea, 6-9 parts of monopotassium phosphate, 5-7 parts of calcium superphosphate, 12-18 parts of potassium nitrate, 5-7 parts of ammonium nitrate, 0.8-1.2 parts of borax, 0.8-1.2 parts of zinc sulfate, 0.8-1.2 parts of manganese sulfate, 0.8-1.2 parts of magnesium sulfate, 6-10 parts of potassium humate, 0.8-1.2 parts of polyglutamic acid, 80-120 parts of water and 45-55 parts of melon ferment stock solution, stirring until the fertilizer is completely dissolved, and continuing to ferment at normal temperature for 1-3 days to obtain the ferment liquid fertilizer.
2. The melon growth-promoting enzyme liquid fertilizer according to claim 1, wherein in the step (2), the microbial agent is formed by mixing rhodopseudomonas palustris, a bacillus subtilis agent and a lactic acid bacteria agent according to a volume ratio of 3-6: 0.8-1.2, wherein the rhodopseudomonas palustris contains 1.8-2.2 hundred million/ml effective viable bacteria, the bacillus subtilis effective viable bacteria is 1800-2200 hundred million/g, and the lactic acid bacteria agent effective viable bacteria is 18-22 hundred million/g.
3. The melon growth promoting enzyme liquid fertilizer according to claim 1, wherein in the step (3), stirring is performed 1-2 times per day within the first month of starting fermentation; and (4) continuing to ferment until no fermentation gas is generated, sealing the fermentation container, placing the fermentation container in a shade place, continuing to ferment for 2-5 months, and filtering out the crushed slag to obtain the fermented soybean milk.
4. A greenhouse melon cultivation fertilizing method is characterized in that after melon seedlings are planted, 350-450 ml/plant of enzyme liquid fertilizer diluent is respectively poured to seedlings on 0 th day, 20 th day, 60 th day and 80 th day, and the enzyme liquid fertilizer diluent is prepared by mixing the enzyme liquid fertilizer and water according to the proportion of 1: 100-800 volume ratio.
5. The greenhouse melon cultivation fertilizing method is characterized in that after 1 month of melon seedling field planting, 800-1200 times of enzyme liquid fertilizer diluent is sprayed to melon seedling leaves every 15 days, 280-320 ml/plant is sprayed for the first time, 380-420 ml/plant is sprayed for the second time, 450-550 ml/plant is sprayed for the third time, and 450-550 ml/plant is sprayed for the fourth time.
CN202011306100.9A 2020-11-19 2020-11-19 Melon growth-promoting enzyme liquid fertilizer and application thereof Pending CN112500222A (en)

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
CN113151559A (en) * 2021-04-28 2021-07-23 河南省农业科学院园艺研究所 Method for identifying purity of muskmelon hybrid seeds
CN114258750A (en) * 2021-12-21 2022-04-01 上海市农业科学院 Method for improving strawberry continuous cropping obstacle soil

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113151559A (en) * 2021-04-28 2021-07-23 河南省农业科学院园艺研究所 Method for identifying purity of muskmelon hybrid seeds
CN113151559B (en) * 2021-04-28 2023-06-13 河南省农业科学院园艺研究所 Method for identifying purity of muskmelon hybrid seeds
CN114258750A (en) * 2021-12-21 2022-04-01 上海市农业科学院 Method for improving strawberry continuous cropping obstacle soil
CN114258750B (en) * 2021-12-21 2023-03-14 上海市农业科学院 Method for improving strawberry continuous cropping obstacle soil

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