KR102010826B1 - Alcaligenes faecalis GA41 having the activity of Promoting Growth of Ginseng and Use Thereof - Google Patents

Abstract

The present invention relates to an alkali Genes Packaris strain having a ginseng growth promoting activity and its use, and more particularly to an alkali Genes Packaris strain having a growth promoting activity of ginseng seeds or ginseng roots and its use.

Description

Alcaligenes faecalis GA41 having the activity of Promoting Growth of Ginseng and Use Thereof}

The present invention relates to an alkali Genes Packaris strain having a ginseng growth promoting activity and its use, and more particularly to an alkali Genes Packaris strain having a growth promoting activity of ginseng seeds or ginseng roots and its use.

Korean ginseng is Korea's unique natural resource, and it is a representative herbal medicine of Korea, which has been widely used for thousands of years as an oriental medicine or medicine for the health of mankind. As Western medicine has shown limitations in overcoming the side effects of synthetic drugs and intractable diseases, the value of herbal medicines and natural products including ginseng is emerging. Ginseng prevents cancer, inhibits aging, protects the liver, strengthens fatigue and improves brain function, and recently, it has been reported to prevent the damage of environmental hormones. Ginseng, the chief of all herbal medicines, is a plant that grows in extremely limited environments. It has a longer growth period than other crops, and its cultivation method is special and difficult, and has been produced only in some countries of the world.

Korea is a ginseng seeding country that produces Korean ginseng with excellent medicinal effects due to its climate and climate suitable for its natural growth and cultivation. However, many countries are interested in the ideal condition that ginseng has as a health food or health drug, and not only existing ginseng producing countries but also non-producing countries are actively promoting policies to encourage the research and production of ginseng. Ginseng growers include Korea, China, Japan, the United States, and parts of Europe. In the past, most of the countries that consumed ginseng were Confucian culture, including Korea, China, Japan, Taiwan, and Southeast Asia. This is expanding. However, Korean ginseng has been weakening its international competitiveness in terms of price, and in order to strengthen its international competitiveness, research on the development of cultivation technology for improving the quality of raw ginseng is necessary. In particular, the most urgent problem in the recent ginseng cultivation is the lack of proper scheduled land, high incidence due to deteriorated soil conditions, and many pesticides cannot be exported due to residual pesticides due to the increase of pesticide use. Intensive research is urgently needed. In addition, intensive use of chemical fertilizers for high yields causes problems such as soil productivity and environmental pollution, and consumers prefer eco-friendly agricultural products without chemical pesticides and chemical fertilizers.

Ginseng is a crop that is cultivated under the Ginseng Industry Law. It is an inorganic fertilizer containing one or more of the components of potassium phosphate during the cultivation process and cannot use fertilizer produced by chemical or physical action. Therefore, organic fertilizers must be used to grow ginseng, and it is known that cultivation and growth management are more difficult than other crops.

The nutrient management of ginseng uses the method of cultivating flowering plants (Sudangrass, corn, wheat, etc.), legume crops, and sweet potatoes on the planned ginseng cultivation site and rotating them three to four times and grinding them deeply. Since organics require a lot of time and labor to make crops available, there is a need to develop technologies to replace them.

IAA (indole-3-actic acid) produced by useful microorganisms is widely distributed in the plant system among natural auxins and is a typical diffusion type auxin with polar movement. It can divide and form irregular roots, promote germination and kidney growth of pollen, form entourage or buds. It is known to play a role in promoting rooting. Recently, microorganisms having plant growth promoting effects have been developed through the use of IAA, siderpores and phosphate availability produced by useful microorganisms, but the field application research on ginseng is insufficient.

Therefore, the present inventors have made an effort to select a microorganism having a ginseng growth promoting effect, as a result, selected Alkali genes Packaris strain having a ginseng growth promoting effect, excellent auxin production ability and having a growth promoting effect on ginseng seeds and roots It confirmed that it and completed this invention.

The present invention has been made to solve the above problems, the first object of the present invention is to provide an alkali Genes Packaris strain having growth promoting activity of ginseng seed or ginseng root.

In addition, an object of the present invention is to provide a strain of Alcaligenes Packaris having auxin (Auxin) production capacity, siderophore production capacity or phosphoric acid availability.

In addition to this, an object of the present invention is to provide a microbial preparation comprising an alkali Genes Packaris strain having ginseng growth promoting activity.

In order to solve the above problems, the present invention provides an alkali Genes Packaris strain of Accession No. KFCC11745P.

In one preferred embodiment of the present invention, the strain may have ginseng growth promoting activity.

In one preferred embodiment of the present invention, the strain may have a growth promoting activity of ginseng seed or ginseng root.

In one preferred embodiment of the present invention, the strain may be a strain for microbial preparation for promoting ginseng growth.

In a preferred embodiment of the present invention, the strain may have auxin (Auxin) generating ability, siderophore generating ability or phosphoric acid availability.

In one preferred embodiment of the present invention, the strain may comprise the 16S rRNA base sequence of SEQ ID NO: 1.

On the other hand, the present invention provides a microbial agent for promoting ginseng growth, including the above-mentioned Caligenes packaris strain, its culture or a mixture thereof as an active ingredient.

In another aspect, the present invention provides a method for promoting ginseng growth, characterized in that the use of a microbial agent for promoting ginseng growth, including the above-mentioned Caligenes packaris strain, its culture or a mixture thereof as an active ingredient.

In one preferred embodiment of the present invention, the culture medium is the strain Luria Bertani (LB) medium, nutrient broth (NB) medium, brain heart infusion (BHI) medium, King's medium B (KB) or Tryptic Soy Broth (TSB) It may be obtained by culturing at 20 ℃ to 40 ℃ in any one or more medium selected from the group consisting of the medium.

As a preferred embodiment of the present invention, the method for promoting ginseng growth may increase the stem length of the ginseng seed 6 to 10%, the root length of the ginseng seed 10 to 14%, the weight of the ginseng seed 3 to 7%. .

In a preferred embodiment of the present invention, the method for promoting ginseng growth may increase the root weight of ginseng root, 30-40%, root diameter 6-10%, root length 4-8%.

Alkali genes Packaris GA41 having a ginseng growth promoting activity of the present invention and its use has biofertilizer activities such as auxin production, siderophore production capacity and phosphoric acid solubility, ginseng growth It has an excellent effect on promoting it.

1 is a diagram showing the and culture characteristics of GA41 strain having ginseng growth promoting activity selected in the present invention.
Figure 2 is a data showing the taxonomic location of the strain by analyzing the phylogeny of GA41 strains selected in the present disease compared to other microbial strains.
Figure 3 is a diagram showing the ginseng seed growth promoting activity of the selected strain GA41 in the present invention.
Figure 4 is a diagram showing the ginseng root growth promoting activity of the strain selected in the present invention.
5 is a view showing 16S rRNA nucleotide sequence information of SEQ ID NO: 1 of the GA41 strain selected in the present invention.

Hereinafter, the present invention will be described in more detail.

As mentioned above, ginseng cannot use chemical fertilizers based on nitrogen, phosphoric acid and potassium under the Ginseng Industry Law. Therefore, until now, a seedling method of cultivating crops such as Sudangrass, rye and corn, and crops such as soybeans and sweet potatoes and using them in the ginseng site soil is required. Do.

The present invention includes Alcaligenes faecalis strain of accession number KFCC11745P.

At this time, the strain of the present invention may have a ginseng growth promoting activity, preferably may have a growth promoting activity of ginseng seeds or ginseng root.

In addition, the strain of the present invention may have auxin (Auxin) generating ability, siderophore (generating ability) or phosphoric acid (phosphoric acid) availability.

In the present invention, strains were isolated from soil samples collected from ginseng root zone soils of the national impression area, and GA41 strains having ginseng growth promoting activity were selected.

Selected GA41 strains have a biofertilizer activity, and to confirm this, auxin (Auxin) production capacity, siderophore production capacity and phosphoric acid (availability of phosphoric acid) was confirmed, as shown in Table 1 below The formation of (indole-3-actic acid), the formation of sidelopores and the availability of phosphate were confirmed.

In addition, the selected GA41 strain has ginseng growth promoting activity, in order to confirm this, as a result of confirming the growth promoting activity and growth promoting activity of the ginseng root, ginseng seeds and as shown in Table 3 and Table 4 below It was confirmed that the growth of ginseng root is promoted.

In the present invention, as a result of analyzing the 16S rRNA sequence to identify the selected strain, GA41 strain has a 16S rRNA sequence of SEQ ID NO: 1, it was confirmed that corresponds to the species Alcaligenes faecalis, The strain was named Alkaneness Packaris GA41 (hereinafter referred to as 'GA41 strain') and was deposited with the Korea Microorganism Conservation Center (Accession No. KFCC11745P).

On the other hand, the present invention includes a microbial agent for promoting ginseng growth, including the above-mentioned alkali genesis Packaris strain, the culture medium or a mixture thereof as an active ingredient.

In addition, the present invention includes a method for promoting ginseng growth, characterized in that the above-mentioned alkali genesis packaris strain, a microorganism for promoting ginseng growth comprising a culture medium or a mixture thereof as an active ingredient.

The culture medium of the microorganism preparation for promoting ginseng growth and the method for promoting ginseng growth of the present invention is the above-mentioned alkaline genes Packaris strain Luria Bertani (LB) medium, nutrient broth (NB) medium, brain heart infusion (BHI) medium, KB ( Can be cultured in any one or more medium selected from the group consisting of King's medium B) or TSB (Tryptic Soy Broth) medium, but if the medium that can maintain a high ginseng growth promoting activity of the Alkali ness Packaris strain of the present invention is limited Can be used without

In addition, the culture medium may have a culture temperature of 20 ℃ to 40 ℃, preferably a culture temperature of 25 ℃ to 35 ℃, but is not necessarily limited to this, the ginseng growth promoting activity of the alkali Genes Packaris strain of the present invention If the culture temperature conditions that can maintain the high can be used without limitation. However, if the culture temperature is less than 20 ℃, mycelial growth is very slow may cause a problem to be cultured for a long time, if it exceeds 40 ℃ may cause problems of mycelial growth inhibition and strain killing during long-term culture.

On the other hand, the method of promoting ginseng growth of the present invention can increase the stem length of the ginseng seed 6% or more, preferably 6-10%, more preferably 7-9%. In addition, the root length of the ginseng seed may be increased by 10% or more, preferably 10-14%, more preferably 11-13%. In addition, the weight of ginseng seed may be increased by 3% or more, preferably 3-7%, more preferably 4-6%.

Furthermore, the method for promoting ginseng growth of the present invention may increase the root weight of ginseng root by more than 30%, preferably 30 to 40%, more preferably 32 to 35%. In addition, the root diameter of the ginseng root may be increased by 6% or more, preferably 6-10%, more preferably 7-9%. In addition, the root length of the ginseng root can be increased by 4% or more, preferably 4-8%, more preferably 5-7%.

Hereinafter, the present invention will be described in detail with reference to a preferred embodiment so that those skilled in the art can easily practice the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention.

Example 1: Useful microorganisms having ginseng growth promoting activity

In order to isolate plant growth promoting myobacteria (PGPR), which have a growth promoting effect on ginseng, ginseng rooting soil of 20cm ~ 30cm depth was collected from main ginseng. 10 g of the soil sample collected in 90 ml of sterile physiological saline was added, and the mixture was shaken in a shaking incubator at 180 rpm for 30 minutes, and then diluted in decimal. 100 μl of each dilution was plated in King's B medium and R2A agar medium and incubated at 30 ° C. for 5 days. A single colony showing apparent differences in each medium was purely separated, and the isolated strain was used as a test strain for investigating biofertilizer activity while storing at -70 degrees with 20% glycerol added.

In the same manner as above, 2,000 strains having different colony shapes and colors were isolated from samples collected from 20 ginseng cultivated soils and rhizospheres. The isolates were stored in cryogenic freezers and assayed for phosphate availability, siderophore production, and auxin production using selective medium after passage to LB agar. As a result, GA41 strain, a Gram-negative bacterium having excellent auxin generating effect and promoting ginseng growth, was selected (FIG. 1).

Example 2: Confirmation of biofertilizer activity

(1) Confirmation of auxin generating ability

As described in Example 1, the collected strains for selection of auxin-producing strains were plated in R2A (Reasoner's 2A agar, Difco) agar medium containing 0.1% L-tryptophan and incubated at 30 ° C. for 2 days. Auxin production assay was used to improve Park's method (2010). The Salkowski reagent was sprayed onto the colony grown in R2A medium by spraying with a nebulizer to select pink strains around the flora as auxin-producing primary strains. The selected strains were inoculated in R2A medium (pH7.0) to which 0.1% L-tryptophan was added and incubated for 24 hours at 30 ° C at 180 rpm. The culture solution was centrifuged at 4 ° C. and 4,000 rpm for 15 minutes to settle the cells, and only the supernatant was collected. The collected supernatant was mixed with Salkowski reagent at a ratio of 1: 2 and reacted for 30 minutes, and the absorbance was measured at 535 nm using a spectrophotometer (Cary 300 UV-VIS, Agilent, USA) to confirm the auxin performance. Standard curves were prepared after adding inodole-3-acetic acid (IAA, Sigma, USA) to R2A medium at concentrations of 0, 10, 25, 50, 75, and 100 ppm and measuring the OD at 535 nm.

(2) Checking the sideropor generation capability

As described in Example 1, the siderophore generating ability of the collection strains was determined by Louden et. Assay according to the method of al, (2011). Dissolve 60 mg of CAS in 50 ml of distilled water, add a solution of 73 mg of hexadecyl trimethylammonium bromide in 40 ml of distilled water, add 2.7 mg of iron (III) chloridehexahydrate to 10 ml of 10 mM Hcl, mix well, and then sterilize blue dye to prepare a blue dye. It was. Distilled water 750ml, agar 15g, 10X MM9 salt 100ml, PIPES 32.24g, 10g casamino acid solution 30ml, adjusted to pH 6.8 by mixing with vitamin and carbon source, autoclaved to cool to around 50 ℃ and then mixed with blue dye It prepared. Purely isolated strains were inoculated in CAS (Chrome Azurol Sulfonate) medium and incubated at 30 ° C. for 1 week to examine the presence or absence of yellow strips around the flora to determine the siderophore generation ability.

(3) Phosphate Solubility Assay

As described in Example 1, the insoluble phosphate solubility was confirmed by plate assay using Pikovskaya agar medium (Himedia, India) to which 0.5% Ca (PO4) was added (Chatlietal, 2008). Strains purely isolated from the rhizosphere were inoculated in Pikovskaya agar medium and cultured for one week at 30 ° C to form a zona pellucida around the flora, and the presence or absence of phosphate availability was determined. The first selected strains were inoculated equilibrated in Pikovskaya agar medium at 4 cm in length, and cultured at 30 ° C. for 5 days to investigate the size of the zona pellucida.

(4) results

Confirmation of the auxin production ability, sideropore production ability and phosphate solubility assay results are shown in Table 1 below.

division IAA production (μg / ml) Create Sidelopor Phosphate Availability GA41 strain 161.44 + + ^a

* ^a Symbol:-, +, ++ indicate no (<1mm), moderate (1-3mm) and strong (> 3mm in clear zone) activity, respectively.

As shown in Table 1, the GA41 strain was confirmed to have biofertilizer activity, such as IAA formation, sideropore and phosphate availability.

Example 3 Identification of Selected GA41 Strains

Molecular biological methods were used to identify GA41 strains selected in Example 1 of the present invention.

First, the GA41 strains selected in Example 1 were inoculated in 20 ml of LB medium, incubated at 30 ° C. for 1 day, and then centrifuged at 13,000 rpm for 10 minutes to harvest the cells.

Genomic DNA (Genomic DNA) was isolated from the harvested cells using the Qiagen Genomic DNA Extraction Kit (Qiagen, Germany). PCR was performed using the universal primers 27F and 1492R (Macrogen, Korea) shown in Table 2 below to amplify the 16S rRNA gene region.

Primer sequence division Sequence SEQ ID NO: 27F AGAGTTTGATCMTGGCTCAG SEQ ID NO: 2 1492R TACGGYTACCTTGTTACGACTT SEQ ID NO: 3

PCR products were purified using the QIAquick PRC purification kit (Qiagen, Germany), and sequence analysis was performed by Macrogen Co., Ltd., and blast search (Blast) at the National Center for Biotechnology Information (NCBI). The strain was identified through search.

As a result of analyzing the 16S rRNA gene for identification of the selected strain, it was confirmed that the GA41 strain had the 16S rRNA sequence (1471bp) of SEQ ID NO. 1 (FIG. 5).

As a result of homology analysis on NCBI web, it was confirmed that the homology with Alcaligenes faecalis ZJB-09133 (GenBank No. 438851) was 99% high, and the GA41 strain of the present invention was Alcaligenes pacaris. It was confirmed that (Alcaligenesfaecalis) species.

Thus, in the present invention, GA41 strain was named as Alcaligenes Packaris GA41 (hereinafter referred to as 'GA41 strain'), and deposited in the Korea Microorganism Conservation Center (Accession Number KFCC11745P).

Example 4 Schematic Analysis of Selected GA41 Strains

In order to confirm the taxonomic location of the GA41 strain selected in Example 1 of the present invention, the base sequence of the Alkali genesis Packaris standard strain is a server operated by the US National Center for Biological Information (http: //www.ncbi.nlm.nih). .gov) and sorted using the seqman (DNASTAR, USA) program. Aligned sequencing was performed using the MGEGA 4.0 program (Tamura et al., 2011), a schematic was generated using the Neighbor-joining algorithm, and the robustness was confirmed by 1000 iterations of bootstrapping.

As a result, phylogenetic analysis based on 16S rRNA sequences revealed that the selected GA41 strain had a high degree of flexibility with Alcaligenes faecalis ZJB-09133 (GQ438851) and Alcaligenes faecalis JF682513. The Bootstrap value was also found to be 47% (FIG. 2).

Example 5 Confirmation of Growth Promoting Activity of Ginseng Seeds of Selected GA41 Strains

Ginseng seeds were harvested in July 2014 at the National Institute of Horticultural & Herbal Science, Gwangju, Korea. Ginseng seeds were sterilized twice with 1% sodium hypochlorite for 5 minutes and 70% ethanol for 1 minute and washed three times with sterile water. Dispense 40 ml of MS medium containing 1% agar into a square Petri dish (126 × 126 × 20mm) and incline it at 10 degrees. Incubated for 1 month at. The GA41 strain selected in Example 1 was inoculated in R2A medium (pH7.0) to which 0.1% L-tryptophan was added, and the culture was shaken for 24 hours at 30 ° C. at 180 rpm. The culture solution was filtered through a 0.2 ㎛ Membrane filter and treated with 50ul each ginseng seed for 5 weeks at 5 days intervals. The growth promotion activity of ginseng seeds was examined one month after the treatment of the culture medium, and the root and stem growths appeared in the seeds are shown in Table 3 and FIG. 3.

division Stem Length (mm) Root Length (mm) Weight (mg) GA41 strain 26.91 12.11 75 No treatment 24.70 10.72 71

As shown in Table 3, the ginseng seed treated with the GA41 strain was found to increase the root length, stem length and weight than the untreated ginseng seed. Specifically, the ginseng seed treated with the GA41 strain increased about 8% more stem length, increased about 12% more root length, and increased about 5% more weight than the untreated ginseng seed.

Example 6 Confirmation of Growth Promoting Activity of Ginseng Roots of Selected GA41 Strains

Growth test of ginseng roots was carried out in ginseng field located in Somyeon, Negong-gun, Chungbuk. 72 ginseng seedlings from the ginseng cultivation farm in Geumwang-eup, Eumseong-gun, Chungbuk were transplanted in a box (1.8m × 0.9m = 1.62m2). Pest control and other cultivation were carried out according to the Ginseng Standard Cultivation Act. The GA41 strain selected in Example 1 was inoculated in R2A medium to which 0.1% L-tryptophan was added and incubated at 30 ° C. and 180 rpm for 2 days. Strain cultures were first treated from the day of germination of seedlings, diluted 6 log CFU / ml, and treated with irrigation 6 times at a monthly interval of 5L per compartment. Ginseng growth survey is shown in Table 4 and 4 by measuring the root length and weight by harvesting 20 ginseng per compartment after one month of the final treatment of the culture solution.

division Root weight (g) Root diameter (mm) Length (cm) GA41 strain 2.14 6.85 17.52 No treatment 1.6 6.3 16.4

As shown in Table 4, the ginseng treated with the GA41 strain was found to increase in weight, root diameter and root length than untreated ginseng. Specifically, the roots of the ginseng treated with the GA41 strain increased the root weight by about 33%, the root diameter by about 8%, and the root length by about 6%.

As described above, specific parts of the present disclosure have been described in detail, and for those skilled in the art, these specific descriptions are merely preferred embodiments, and the scope of the present disclosure is not limited thereto. Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Korea Microbial Conservation Center (Domestic) KFCC11745P 20171023

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Alcaligens faecalis GA41 having the activity of Promoting Growth          of Ginseng and Use Thereof <130> 1063132 <160> 3 <170> KoPatentIn 3.0 <210> 1 <211> 1471 <212> DNA <213> Alchemilla alpina <400> 1 cgtagattga cgctagcggg atgctttaca catgcaagtc gaacggcagc gcgagagagc 60 ttgctctctt ggcggcgagt ggcggacggg tgagtaatat atcggaacgt gcccagtagc 120 gggggataac tactcgaaag agtggctaat accgcatacg ccctacgggg gaaagggggg 180 gatcgcaaga cctctcacta ttggagcggc cgatatcgga ttagctagtt ggtggggtaa 240 aggctcacca aggcaacgat ccgtagctgg tttgagagga cgaccagcca cactgggact 300 gagacacggc ccagactcct acgggaggca gcagtgggga attttggaca atgggggaaa 360 ccctgatcca gccatcccgc gtgtatgatg aaggccttcg ggttgtaaag tacttttggc 420 agagaagaaa aggtacctcc taatacgagg tactgctgac ggtatctgca gaataagcac 480 cggctaacta cgtgccagca gccgcggtaa tacgtagggt gcaagcgtta atcggaatta 540 ctgggcgtaa agcgtgtgta ggcggttcgg aaagaaagat gtgaaatccc agggctcaac 600 cttggaactg catttttaac tgccgagcta gagtatgtca gaggggggta gaattccacg 660 tgtagcagtg aaatgcgtag atatgtggag gaataccgat ggcgaaggca gccccctggg 720 ataatactga cgctcagaca cgaaagcgtg gggagcaaac aggattagat accctggtag 780 tccacgccct aaacgatgtc aactagctgt tggggccgtt aggccttagt agcgcagcta 840 acgcgtgaag ttgaccgcct ggggagtacg gtcgcaagat taaaactcaa aggaattgac 900 ggggacccgc acaagcggtg gatgatgtgg attaattcga tgcaacgcga aaaaccttac 960 ctacccttga catgtctgga aagccgaaga gatttggccg tgctcgcaag agaaccggaa 1020 cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1080 cgagcgcaac ccttgtcatt agttgctacg caagagcact ctaatgagac tgccggtgac 1140 aaaccggagg aaggtgggga tgacgtcaag tcctcatggc ccttatgggt agggcttcac 1200 acgtcataca atggtcggga cagagggtcg ccaacccgcg agggggagcc aatctcagaa 1260 acccgatcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg aatcgctagt 1320 aatcgcggat cagaatgtcg cggtgaatac gttcccgggt cttgtacaca ccgcccgtca 1380 caccatggga gtgggtttca ccagaagtag gtagcctaac cgtaaggagg gcgcttacca 1440 cggtgggatt catgactggg gtgaatcgaa g 1471 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 22F_primer <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R_primer <400> 3 tacggytacc ttgttacgac tt 22

Claims (10)

It contains the 16S rRNA base sequence of SEQ ID NO: 1, and contains the alkaline genes Packaris strain of Accession No. KFCC11745P having a ginseng growth promoting activity, its culture or a mixture thereof as an active ingredient,
The ginseng growth promoting activity increases the stem length of the ginseng seed 6 ~ 10%, the root length of the ginseng seed 10 ~ 14%, the weight of the ginseng seed 3 ~ 7%, the weight of the ginseng root 30 ~ 40%, Microbial agent for promoting ginseng growth, characterized in that the increase in root length 6 ~ 10%, root length 4 ~ 8%.
delete delete The method of claim 1,
The strain is a microbial agent for promoting ginseng growth, characterized in that it has the ability to produce auxin (Auxin), siderophore (phosphoric acid) or soluble (phosphoric acid).
delete delete The method of claim 1,
The culture medium is any one selected from the group consisting of Luria Bertani (LB) medium, nutrient broth (NB) medium, brain heart infusion (BHI) medium, King's medium B (KB) or Tryptic Soy Broth medium (TSB) medium. Microorganism preparation for promoting ginseng growth, characterized in that obtained by culturing at 20 ℃ to 40 ℃ in the above medium.
Ginseng growth promoting method of using the microorganism agent for promoting ginseng growth of claim 1.


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