CN102329754A - Novel strain of streptomyces fradiae and application thereof - Google Patents
Novel strain of streptomyces fradiae and application thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.
Description
Technical field
The invention belongs to and be used for the soil and fertilizer field, be specifically related to a kind of agricultural microorganism preparation and application thereof.
Background technology
White agriculture is meant the industrial new agricultural of Microbial resources industrialization, is often referred to the application of microbial fermentation engineering on agricultural.Agriculture prodn needs soil that the nutritive substance and the living environment of plant growth are provided.For many years, using of chemical fertilizer played keying action to getting agricultural profit.Yet; Because long-term application chemical fertilizer, the soil beneficial microbe colony is damaged, fertilizer is under-supply, all kinds of nutrients ratio imbalances; Cause farmland ecological environment, soil physical and chemical property and soil microflora to receive destruction in various degree; Influenced the quality of agricultural-food to a certain extent, the present soil resource present situation of China presses for through the people to measure solves soil by destructive problem gradually, guarantees agricultural planting level and crop products quality.But the phosphorus that is difficult to utilize in the nitrogen in some useful microbe fixed airs, the activating soil, potassium and other nutritive elements; Improve soil nutrient deliverability and water conservation, fertilizer-preserving ability; Improve the lasting throughput of land resources, can also strengthen disease-resistant crops property, reduce pesticide dosage.
In ten big countermeasures of China's ecological environmental protection, clearly advocate and to promote " ecological agriculture "; Preserve the ecological environment and agricultural land soil; Promote the production of pollution-free healthy food, promote biological cycle in the ecosystem, reach maintenance and strengthen soil fertility and biological activity purpose.The microbial preparation or the fertilizer product that with the useful microbe are core can play effects such as improving the soil, increase fertility of land, promote growth, are the preferred production means that the high-quality non-polluted farm product is produced.Along with the fast development of China's modern agriculture, particularly the green organic agricultural cultivated area constantly enlarges, and the specified microorganisms bacterium is dissolved into agriculture prodn, the hot issue that has become current government and enterprise and paid close attention to.
Summary of the invention
The present invention at first provides a kind of streptomyces fradiae (Streptomyces fradiae) new bacterial strain CLIMAX-VSF.
The present invention also provides the microbial inoculum that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the seed treatment agent that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the bacterial manure that contains streptomyces fradiae CLIMAX-VSF.
The present invention also provides the sprinkling irrigation that contains streptomyces fradiae CLIMAX-VSF agent.
Streptomyces fradiae CLIMAX-VSF provided by the invention separates from the soil of high-yield field and get, and this bacterial strain ten minutes is disease-resistant significantly, fixing nitrogen, dissolving phosphor and dissolving potassium and secretion growth hormone performance.Carry out preservation at China Committee for Culture Collection of Microorganisms common micro-organisms center, the preservation accession designation number is CGMCC No.5133, and preservation date is on August 12nd, 2011.
The researchist is barren relatively at soil but obtain rhizosphere soil in the cotton soil that output is higher; In soil supension, add phenol number droplet and through selecting culture medium culturing; Get the bacterial classification of compound characteristics and at test under microscope; Cultivate screening through separating repeatedly after proving single CLIMAX-VSF thalline, obtain the CLIMAX-VSF bacterial strain with a large amount of bacterial strain repeatability.The CLIMAX-VSF bacterial strain confirms that through cultural characteristic, microscopic morphology characteristic, physiological and biochemical property and hereditary feature this bacterial strain meets the characteristic of streptomyces fradiae.
Streptomyces fradiae CLIMAX-VSF of the present invention can be prepared into microbial inoculum, and wherein, the viable count of streptomyces fradiae CLIMAX-VSF can be more than 5,000,000,000/gram, is preferably 10,000,000,000/gram.
The microbial inoculum that contains streptomyces fradiae CLIMAX-VSF of the present invention can prepare as follows:
1) preparation streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: the seed liquor of step 1) is linked in the solid multiplication substratum 30 ± 2 ℃ aseptic aerobic conditions and cultivated 7～10 days down; Described solid multiplication substratum is preferably and contains 50～70 parts in wheat bran, 3～6 parts in brown sugar, cottonseed cake or 20～40 parts of soya-bean cakes, 1～3 part in sal epsom, 1～3 part in sodium-chlor, 1～4 part in lime carbonate, pH7.0～7.5;
3) substratum behind the step 3) multiplication culture is carried out Air drying, promptly get the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
The present invention also provides the preparation method who contains streptomyces fradiae CLIMAX-VSF microbial inoculum, and it comprises:
1) preparation streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: the seed liquor of step 1) is linked in the solid multiplication substratum 30 ± 2 ℃ aseptic aerobic conditions and cultivated 7～10 days down; Described solid multiplication substratum contains 50～70 parts in wheat bran, 3～6 parts in brown sugar, cottonseed cake or 20～40 parts of soya-bean cakes, 1～3 part in sal epsom, 1～3 part in sodium-chlor, 1～4 part in lime carbonate, pH7.0～7.5;
3) substratum behind the step 3) multiplication culture is carried out Air drying, promptly get the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
Seed treatment agent provided by the invention, preferably contain the component of following weight part:
Streptomyces fradiae CLIMAX-VSF microbial inoculum 30-90 part;
Carrier 5-30 part;
Medical stone 5-20 part;
Agricultural rare-earth 0-40 part;
Zinc sulfate 0-10 part.
Wherein, described carrier is preferably the mixture of one or more arbitrary proportions in zeolite powder, wilkinite, the Attapulgite.
Bacterial manure provided by the invention, preferably contain the component of following weight part:
Streptomyces fradiae CLIMAX-VSF microbial inoculum 0.2-30 part
Fertilizer 30-100 part
Humic acid powder 10-50 part
N-p-k element 0-50 part.
N-p-k element is meant the NPK raw material commonly used that is used to can be used for agricultural, and the nitrogen element can be selected from urea, ammonium sulfate, ammonium chloride or its combination; Phosphorus can be selected from monoammonium phosphate, diammonium phosphate, superphosphate of lime, potassium primary phosphate, ground phosphate rock or its combination; Potassium is optional from vitriolate of tartar, Repone K, salt of wormwood, saltpetre or its combination.
Sprinkling irrigation agent provided by the invention, preferably contain following weight part ground component:
50～400 parts of streptomyces fradiae CLIMAX-VSF filtered liqs
300～600 parts of aminoacids solutions
10～200 parts of potassium primary phosphates
10～50 parts of boric acid
10～60 parts of manganese sulfate monohydrates
10～50 parts of Zinc Sulphate Heptahydrates
20～150 parts in urea
0～50 part of agricultural rare-earth, wherein, streptomyces fradiae CLIMAX-VSF filtered liq is that streptomyces fradiae CLIMAX-VSF microbial inoculum and water were stirred 1～8 hour after according to 1: 50～200 mixed, filters the filtrating of gained.
Wherein, aminoacids solution can add deionized water with the soluble amino acid powder to be processed, and also can directly buy aminoacids solution.At present above two kinds of raw materials are all available, like Baoding fragrant derived food ltd that distinguishes the flavor of.
The new bacterial strain CLIMAX-VSF of streptomyces fradiae provided by the invention has very disease-resistant significantly, fixed nitrogen phosphorus decomposing and secretion growth hormone performance.This bacterium can not significantly improve percentage of germination, seedling growth speed under soil adds the situation of nutrient, and significantly suppress seedling stage and vegetative period disease.Aspect the synergy that is mixed that is used for fertilizer, demonstrate this bacterium and possess good activating soil nutrient effect and anti-multiple farming obstacle performance.In addition, this bacterium can be made into the liquid product use of spraying through certain process, further with the use three-dimensional of streptomyces fradiae, makes the outer probiotics that can use this kind ecological environment-friendly type of rhizosphere and root.
The present invention carries out value added by manufacture through adopting the liquid+solid combined fermentation method; The mode of adsorbing into solid preparation with traditional liquid fermenting is compared; The sporulation quantity that this method is carried out bacterial classification propagation is higher, the meta-bolites amount is big, and the biological activity of its product is better, and preservation period is longer.
The CLIAMX-VSF streptomyces fradiae is natural yeast culture; Its application has bright prospects; Be used to make seed treatment agent, fertilizer synergist, three kinds of application of sprinkling irrigation agent feasible scheme is provided for the present invention has developed it, and obtain the favorable actual application effect.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Do not specify that " part " described in the present invention is meant " weight part " if having.
The evaluation of embodiment 1 streptomyces fradiae CLIMAX-VSF
The morphological specificity of streptomyces fradiae CLIMAX-VSF of the present invention sees the following form.
Physiological and biochemical property is following:
| Test subject | The result | Test subject | The result | Test subject | The result |
| Glucose | + | Glycerine | + | Sodium malate | + |
| N.F,USP MANNITOL | + | Lactose | + | Sunmorl N 60S | + |
| Saligenin | - | Semi-lactosi | + | Trisodium Citrate | - |
| Raffinose | - | Melizitose | + | Sodium succinate | - |
| Starch | + | L-arabinose | + | Sodium malonate | - |
| Sorbyl alcohol | - | Wood sugar | + | Sodium hippurate | - |
| Sorbose | - | Cellobiose | + | Sodium Propionate | - |
| Sucrose | - | Tetrahydroxybutane | + | Sodium acetate | + |
| Melibiose | + | Amygdaloside | + | Sodium tartrate | - |
| Seminose | + | Trehalose | + | The casein amino acid enzyme | + |
| SANMALT-S | - | Ribose | + | Glycase | + |
| Synanthrin | - | Glycogen | - | Gelatine liquefication | - |
| Rhamnosyl | - | Inositol | - | Nitrate reduction | + |
| Fructose | + | Galactitol | + | ||
Measured the sequence of the 16sRNA of streptomyces fradiae CLIMAX-VSF, sequence is shown in SEQ ID No.1.Through comparison, higher with the sequence homology of the 16sRNA of the streptomyces fradiae of other announcement.
Through above-mentioned morphological specificity, physiological ecological characteristic and 16sRNA sequential analysis, can confirm to be separated to and be the new bacterial strain of streptomyces fradiae really.
The biological characteristics of embodiment 2 streptomyces fradiae CLIMAX-VSF
1. secrete the growth hormone performance
1) the freeze-drying pipe that will preserve streptomyces fradiae CLIMAX-VSF earlier carries out activation.Concrete grammar is to be linked into earlier in the inclined-plane of Gause I substratum to cultivate 15 days at 30 ± 2 ℃; Behind the bacterium colony that secures good health; It is inserted in the PDA substratum 30 ℃ cultivated 5 days; Select then grow healthy bacterial classification be linked into through sterilization by in 20 parts of sucrose, 5 parts in sodium-chlor, 20 parts of peptones, 5 parts in sal epsom, 3 parts of potassium primary phosphates, 1000 parts of liquid nutrient mediums of forming of water; And then 150r/min, 30 ± 2 ℃ cultivated 3 days, obtains the streptomyces fradiae seed liquor.
2) solid multiplication is cultivated: the streptomyces fradiae seed liquor is linked into by in 60 parts in wheat bran, 5 parts in brown sugar, 30 parts of cottonseed cakes, 1.5 parts in sal epsom, 1.5 parts in sodium-chlor, 2 parts of solid culture mediums (pH7.5) through high-temperature sterilization more than 120 ℃ of forming of lime carbonate; Under 30 ± 2 ℃ aseptic aerobic conditions, cultivate;, viable count carries out the growth hormone horizontal checkout when being 1,000,000,000/gram at solid multiplication); Find that its secreted plant hormone level is higher, the result sees the following form.
Table 1 streptomyces fradiae excretory growth hormone kind and level
| 1,000,000,000/gram | |
| Plant hormones regulators,gibberellins GA3 | 103.37mg/kg |
| Indolylacetic acid IAA | 4.82mg/kg |
| Phytokinin CTK | 2.61mg/kg |
2. anti-microbial property
Test through inhibition zone: through in petridish, inserting CLIMAX-VSF bacterial classification and pathogenic bacteria, test its white space diameter, big more this bacterium of explanation of diameter range is strong more to the inhibition ability of pathogenic bacteria:
The anti-microbial activity of table 2 streptomyces fradiae CLIMAX-VSF
| Pathogenic bacteria | Bacteriostatic diameter (mm) |
| The root rotof flax bacterium | 29 |
| Verticillium dahliae | 20.5 |
| Cotton-wilt fusarium | 20 |
| The cotton anthracnose bacterium | 27 |
| The cucumber phytophthora mycosis | 28 |
| Folium Mali pumilae zonate spot bacterium | 19 |
| The willow anthrax bacteria | 35.5 |
| The bacterial wilt of peanut bacterium | 27 |
| The sweet potato bacterial wilt bacterium | 33.5 |
| Watermelon anthrax bacteria | 39.5 |
3. it is active to separate the nitrogen phosphorus decomposing
Follow the tracks of plant through long-term field test through streptomyces fradiae CLIMAX-VSF seed treatment and soil inoculation; Compare at lean soil, not fertilising state; Find that it has tangible effect with insoluble phosphorus dissolved effect in the soil and air fixed nitrogen; It is compared with the contrast blank will increase production 16.7% and 37.2% respectively, and the supply that demonstrates nitrogen element and phosphoric all obviously is better than the contrast blank.
The preparation of the former bacteria agent of embodiment 3 streptomyces fradiae CLIMAX-VSF
The present invention adopts liquid-solid combined ferment culture process to carry out the cultivation of streptomyces fradiae microbial inoculum, is linked into the solid culture multiplication culture again through first liquid multiplication culture, and formed microbial inoculum has very strong bioenzyme activity, and concrete steps comprise:
1) seed preparation and liquid multiplication culture: the freeze-drying pipe that will preserve streptomyces fradiae CLIMAX-VSF earlier carries out activation.Concrete grammar is to be linked into earlier in the inclined-plane of Gause I substratum to cultivate 15 days at 30 ± 2 ℃; Behind the bacterium colony that secures good health; It is inserted in the PDA substratum 30 ℃ cultivated 5 days; Select then grow healthy bacterial classification be linked into through sterilization by in 20 parts of sucrose, 5 parts in sodium-chlor, 20 parts of peptones, 5 parts in sal epsom, 3 parts of potassium primary phosphates, 1000 parts of liquid nutrient mediums of forming of water; And then 150r/min, 30 ± 2 ℃ cultivated 3 days, obtains the streptomyces fradiae seed liquor.
2) solid multiplication is cultivated: the streptomyces fradiae seed liquor is linked into by in 60 parts in wheat bran, 5 parts in brown sugar, 30 parts of cottonseed cakes, 1.5 parts in sal epsom, 1.5 parts in sodium-chlor, 2 parts of solid culture mediums (pH7.5) through high-temperature sterilization more than 120 ℃ of forming of lime carbonate, under 30 ± 2 ℃ aseptic aerobic conditions, cultivated 7 days.
3) drying treatment: after solid multiplication is cultivated and finished it is put in kiln and carries out Air drying.It is 8,000,000,000/g that dried streptomyces fradiae detects living bacteria count through colony counting method, and it is preserved as the former bacteria preparation packing of streptomyces fradiae warehouse-in.
Embodiment 4 contains the seed treatment agent of streptomyces fradiae CLIMAX-VSF
30 parts of the former bacteria preparations of streptomyces fradiae, 5 parts in carrier (zeolite powder), 20 parts of medical stones, 10 parts in the zinc sulfate of embodiment 3 preparations, above raw material thorough mixing evenly promptly gets.
Embodiment 5 contains the seed treatment agent of streptomyces fradiae CLIMAX-VSF
(wilkinite: Attapulgite 1: 1) 30 parts, 5 parts of medical stones, 40 parts of agricultural rare-earths, above raw material thorough mixing evenly promptly gets for 90 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, carrier.
Embodiment 6 contains the seed treatment agent of streptomyces fradiae CLIMAX-VSF
60 parts of the former bacteria preparations of streptomyces fradiae, 10 parts in carrier (wilkinite), 10 parts of medical stones, 20 parts of the agricultural rare-earths of embodiment 3 preparations, 5 parts in zinc sulfate, above raw material thorough mixing evenly promptly gets.
Embodiment 7 contains the bacterial manure of streptomyces fradiae CLIMAX-VSF
2 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, 100 parts of fertilizers, 10 parts of humic acid powder, urea by 10 parts in nitrogen, diammonium phosphate by 20 parts in phosphorus, Repone K by 20 parts in potassium, the above-mentioned raw materials thorough mixing is promptly got the bacterial manure that contains streptomyces fradiae CLIMAX-VSF after evenly.
Embodiment 8 contains the bacterial manure of streptomyces fradiae CLIMAX-VSF
30 parts of the former bacteria preparations of streptomyces fradiae, 30 parts of fertilizers, 50 parts of the humic acid powder of embodiment 3 preparation promptly get the bacterial manure that contains streptomyces fradiae CLIMAX-VSF with the above-mentioned raw materials thorough mixing after evenly.
Embodiment 9 contains the bacterial manure of streptomyces fradiae CLIMAX-VSF
15 parts of the former bacteria preparations of streptomyces fradiae of embodiment 3 preparation, 50 parts of fertilizers, 30 parts of humic acid powder, ammonium chloride by 10 parts in nitrogen, superphosphate of lime by 10 parts in phosphorus, vitriolate of tartar by 10 parts in potassium, the above-mentioned raw materials thorough mixing is promptly got the bacterial manure that contains streptomyces fradiae CLIMAX-VSF after evenly.
Embodiment 10 contains the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 is carried out intermittent type and stirs according to adding water at 1: 50; Stirred 5 hours; Stirring velocity is 60rpm, then it is filtered the streptomyces fradiae filtered liq that the back obtains to be rich in thalline and compositions such as plurality of enzymes and growth hormone, the following prescription of preparation:
2) earlier with 300 parts of aminoacids solutions (available from Baoding fragrant derived food ltd that distinguishes the flavor of), 200 parts of potassium primary phosphates, 10 parts of boric acid, 60 parts of manganese sulfate monohydrates, 10 parts of Zinc Sulphate Heptahydrates, 20 parts in urea, 0.001 part in tensio-active agent.Above composition mixed in retort 30 minutes at 60 ℃, treated that temperature is reduced to below 25 ℃ to add 50 parts of streptomyces fradiae filtered liqs, and stirring at normal temperature was processed the sprinkling irrigation agent in 20 minutes again.
Embodiment 11 contains the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 is carried out intermittent type and stirs according to adding water at 1: 200; Stirred 8 hours; Stirring velocity is 70rpm, then it is filtered the streptomyces fradiae filtered liq that the back obtains to be rich in thalline and compositions such as plurality of enzymes and growth hormone, the following prescription of preparation:
2) earlier with 600 parts of aminoacids solutions (available from Baoding fragrant derived food ltd that distinguishes the flavor of), 10 parts of potassium primary phosphates, 50 parts of boric acid, 10 parts of manganese sulfate monohydrates, 50 parts of Zinc Sulphate Heptahydrates, 150 parts in urea, 50 parts of agricultural rare-earths, 0.002 part in tensio-active agent.Above composition mixed in retort 60 minutes at 60 ℃, treated that temperature is reduced to below 25 ℃ to add 400 parts of streptomyces fradiae filtered liqs, and stirring at normal temperature was processed the sprinkling irrigation agent in 5 minutes again.
Embodiment 12 contains the sprinkling irrigation agent of streptomyces fradiae CLIMAX-VSF
1) the former bacteria preparation of streptomyces fradiae CLIMAX-VSF of embodiment 3 is carried out intermittent type and stirs according to adding water at 1: 100; Stirred 6 hours; Stirring velocity is 60rpm, then it is filtered the streptomyces fradiae filtered liq that the back obtains to be rich in thalline and compositions such as plurality of enzymes and growth hormone, the following prescription of preparation:
2) earlier with aminoacids solution (available from Baoding distinguish the flavor of 400 parts in fragrant derived food ltd, 100 parts of potassium primary phosphates, 30 parts of boric acid, 30 parts of manganese sulfate monohydrates, 25 parts of Zinc Sulphate Heptahydrates, 80 parts in urea, 20 parts of agricultural rare-earths, 0.2 part in tensio-active agent.Above composition mixed in retort 40 minutes at 80 ℃, treated that temperature is reduced to below 25 ℃ to add 200 parts of streptomyces fradiae filtered liqs, and stirring at normal temperature was processed the sprinkling irrigation agent in 10 minutes again.
Test Example 1 seed treatment agent
Before rice growing, with the seed treatment agent of embodiment 4～6 to the rice paddy seed processing of soaking seed.The seed rice of every mu of consumption uses 20 grams to add and soaks seed after 5 kg of water stir, and is after planting investigating discovery about 2 weeks, compares with the clear water seed soaking, and rice shoot is healthy and strong, and plant height, root length increase, and grown in field is vigorous, and setting percentage and thousand seed weight are improved.Output on average increases by 10.5%, meanwhile, also has the bakanae disease of control effect through field observation.
Test Example 2 bacterial manure
In the base fertilizer phase, the bacterial manure of 80 kilograms of embodiment 7 of every mu of even ditch spread of grape.Identical with embodiment 7 prescription, but the contrast that does not contain the former bacteria preparation of streptomyces fradiae compares, and can pluck in advance 5 days after using, amount of increase in production reaches 18.1% when plucking simultaneously, and significantly improves the commodity performance, fruit look bright-coloured gloss, and sugariness increases more than 2 degree.Embodiment 7 and 8 bacterial manure also have similar effects.
Test Example 3 sprinkling irrigation agent
Behind the slow seedling of tomato field planting, respectively spray with fast growing period and combine root to irritate the sprinkling irrigation agent of embodiment 10, wherein, each 200 milliliters spray according to water distribution in 1: 75, in conjunction with 5 kilograms of every mu of usefulness according to irritating root at 1: 200 than water.Find through tracking investigation, compare with the clear water contrast, after the use, leaf viral disease and virus disease disease index decline 40-70%; Average raising the output 15-20% reach as high as 33%, and the stature of tomato is even; The fruit look healthy, and single fruit representation work increases, and has good economic benefit.Embodiment 10 and 11 sprinkling irrigation agent also have similar effects.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (7)
1. the new bacterial strain CLIMAX-VSF of a streptomyces fradiae (Streptomyces fradiae), its preserving number is CGMCC No.5133.
2. the microbial inoculum that contains the said bacterial strain of claim 1.
3. microbial inoculum according to claim 2, wherein the viable count of streptomyces fradiae CLIMAX-VSF is more than 5,000,000,000/gram.
4. according to claim 2 or 3 described microbial inoculums, it is characterized in that, prepare as follows:
1) preparation streptomyces fradiae CLIMAX-VSF seed liquor;
2) solid multiplication of streptomyces fradiae CLIMAX-VSF is cultivated: the seed liquor of step 1) be linked in the solid multiplication substratum 30 ± 2 ℃ aseptic aerobic conditions cultivate down 7～10 days to viable count be more than 5,000,000,000/gram; Described solid multiplication substratum contains 50～70 parts in wheat bran, 3～6 parts in brown sugar, cottonseed cake or 20～40 parts of soya-bean cakes, 1～3 part in sal epsom, 1～3 part in sodium-chlor, 1～4 part in lime carbonate, pH7.0～7.5;
3) substratum behind the step 3) multiplication culture is carried out drying, promptly get the former bacteria agent of streptomyces fradiae CLIMAX-VSF.
5. contain the seed treatment agent of the said bacterial strain of claim 1, it is characterized in that, contain the component of following weight part:
The described microbial inoculum 30-90 of claim 4 part;
Carrier 5-30 part;
Medical stone 5-20 part;
Agricultural rare-earth 0-40 part;
Zinc sulfate 0-10 part.
6. contain the bacterial manure of the said bacterial strain of claim 1, it is characterized in that, contain the component of following weight part:
The described microbial inoculum 0.2-30 of claim 4 part;
Fertilizer 30-100 part;
Humic acid powder 10-50 part;
N-p-k element 0-50 part.
7. contain the sprinkling irrigation agent of the said bacterial strain of claim 1, it is characterized in that, contain following weight part ground component:
50～400 parts of streptomyces fradiae CLIMAX-VSF filtered liqs;
300～600 parts of aminoacids solutions;
10～200 parts of potassium primary phosphates;
10～50 parts of boric acid;
10～60 parts of manganese sulfate monohydrates;
10～50 parts of Zinc Sulphate Heptahydrates;
20～150 parts in urea;
0～50 part of agricultural rare-earth
0.001～0.2 part in tensio-active agent,
Wherein, streptomyces fradiae CLIMAX-VSF filtered liq is according to 1: 50～200 the mixed filtrating of after-filtration gained that stirs with the described microbial inoculum of claim 4 and water.
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