CN109956802A - A kind of liquid fertilizer and preparation method thereof that fruit corn yield can be improved - Google Patents
A kind of liquid fertilizer and preparation method thereof that fruit corn yield can be improved Download PDFInfo
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- CN109956802A CN109956802A CN201711426600.4A CN201711426600A CN109956802A CN 109956802 A CN109956802 A CN 109956802A CN 201711426600 A CN201711426600 A CN 201711426600A CN 109956802 A CN109956802 A CN 109956802A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a kind of liquid fertilizer and preparation method thereof that fruit corn yield can be improved, which includes 1000 parts of active liquid fertilizer substrate preparation, 5 ~ 15 parts of chitosan oligosaccharide, 80 ~ 120 parts of urea, poly- 10 ~ 20 parts of asparagus fern door propylhomoserin, phenylalanine-3,4-quinone ~ 10 part, 130 ~ 168 parts of potassium sulfate, 80 ~ 105 parts of calcium superphosphate, 1 ~ 10 part of boric acid, 0.1 ~ 5 part of calcium gluconate, 1 ~ 10 part of white vitriol；The liquid fertilizer has not only effectively facilitated the flowers and fruits growth of fruit corn, has selectivity to the production-increasing function of different corns while improving yield, the most significant to the production-increasing function of fruit corn.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid that fruit corn yield can be improved
Body fertilizer and preparation method thereof.
Background technique
Fruit corn is a kind of super-sweet corn for being suitble to eat it raw, and green stick stage thin skin, juice is more, matter is crisp and sweet tea, can directly be given birth to
Eat, for very thin epidermis once stinging brokenly, the juice of faint scent overflows tooth cheek, eat it raw it is ripe eat all special sweet tea, especially crisp, as fruit,
Therefore it is referred to as " fruit corn ".Fruit corn belongs to one of corn, is the developed countries such as American-European, South Korea and Japan
One of main vegetables, because that it is with nutrition abundant, sweet tea, fresh, crisp, tender characteristic is deep by each stratum consumers.Production
In corn can be divided into conventional corn, super-sweet corn and reinforced corn three classes, super-sweet corn is high, suitable due to sugar content
Suitable picking time is long and is planted extensively.Corn also has cultivation in China.Plant is shorter, and tillering ability is stronger.On fruit ear bract
There is boot leaf.Seed is yellowish or white, and embryo is larger, and milk stage is tender in fact, is rich in water-soluble polysaccharide, vitamin A, vitamin C, rouge
Fat and protein etc..
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
Fruit corn often occurs blooming less, ear less, the phenomenon of inane fruit etc., seriously affected the yield of fruit corn with
And fruit quality, in order to reduce plant body in cultivation lack nutrition storage, occur earing less, inane fruit phenomena such as, Ying Jiaqiang
The supplement of nutrient is planted, the application of the nutritious fertilizer in cultivation directly affects fruit development and yield.
Summary of the invention
The technical problem to be solved by the present invention is to overcome ear less in existing fruit corn cultivation, inane fruit it is more, provide
A kind of liquid fertilizer and preparation method thereof that fruit corn yield can be improved.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer that fruit corn yield can be improved, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation, 5~15 parts of chitosan oligosaccharide, 80~120 parts of urea, poly- asparagus fern door propylhomoserin 10~
20 parts, phenylalanine-3,4-quinone~10 part, 130~168 parts of potassium sulfate, 80~105 parts of calcium superphosphate, 1~10 part of boric acid, gluconic acid
0.1~5 part of calcium, 1~10 part of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=
0.4mg/g, the content of organic matter are 120~165g/L.
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain
The culture medium of " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, chitosan oligosaccharide 8~
12 parts, 90~110 parts of urea, poly- 12~18 parts of asparagus fern door propylhomoserin, 5~8 parts of phenylalanine, 145~153 parts of potassium sulfate, excessively phosphorus
90~100 parts of sour calcium, 3~8 parts of boric acid, 0.5~3 part of calcium gluconate, 2~8 parts of white vitriol；It is total in the liquid fertilizer
Nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 120~135g/L.
Preferably, the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, chitosan oligosaccharide 10
Part, 98 parts of urea, poly- 15 parts of asparagus fern door propylhomoserin, 6 parts of phenylalanine, 148 parts of potassium sulfate, 92 parts of calcium superphosphate, 6 parts of boric acid, Portugal
2 parts of grape Calciofon, 6 parts of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/
G, the content of organic matter are 128~132g/L.
Further, the weight ratio of N, P, K are 8~15:5:12~16, preferably 13:5:14 in the liquid fertilizer.
Further, the ratio between the boric acid, calcium gluconate, white vitriol number are 3:1:3.
A kind of preparation method of the liquid fertilizer that fruit corn yield can be improved, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. chitosan oligosaccharide, urea, poly- asparagus fern door ammonia the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Acid, phenylalanine, potassium sulfate, calcium superphosphate, boric acid, calcium gluconate, white vitriol obtain the liquid fertilizer.
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.5g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer of the invention can effectively facilitate corn fruit development, ensure that corn Fruit is full, can be significant
Reduce inane fruit, flat fruit.
Liquid fertilizer of the invention increases 66.7% to the flower number of fruit corn, fruit-setting rate 80.0%, relative comparison
Group increases 60.0%, and tool increases significantly.The single fresh fruit weight of the fruit corn of fluid present invention fertilizer has been used also to increase
46.6%, realize that per mu yield increases nearly three times, effect is considerable.However, flower number of the liquid fertilizer to black waxy corn
Amount and the facilitation effect of fruit-setting rate all do not play substantive effect, to the fruit retention rate of black waxy corn, per mu yield and fruit product
Matter increases only more small effect.Liquid fertilizer of the invention has very significant effect to the flower and fruit protecting of fruit corn
Fruit, to the flower and fruit protecting of the corn of different cultivars selectivity with higher.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
The embodiment 2 a kind of preparation and application of the liquid fertilizer that fruit corn yield can be improved
A kind of preparation method for the liquid fertilizer that fruit corn yield can be improved the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. the configuration of liquid fertilizer: into 1000 parts of active liquid fertilizer substrate preparations add 5 parts of chitosan oligosaccharide, 80 parts of urea,
Poly- 10 parts of asparagus fern door propylhomoserin, phenylalanine-3,4-quinone part, 130 parts of potassium sulfate, 80 parts of calcium superphosphate, 1 part of boric acid, calcium gluconate 0.1
Part, 1 part of white vitriol obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 120g/L.
The embodiment 3 a kind of preparation and application of the liquid fertilizer that fruit corn yield can be improved
A kind of preparation method for the liquid fertilizer that fruit corn yield can be improved the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. the configuration of liquid fertilizer: into 1000 parts of active liquid fertilizer substrate preparations add 8 parts of chitosan oligosaccharide, 90 parts of urea,
Poly- 12 parts of asparagus fern door propylhomoserin, 5 parts of phenylalanine, 145 parts of potassium sulfate, 90 parts of calcium superphosphate, 3 parts of boric acid, calcium gluconate 0.5
Part, 2 parts of white vitriol obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 123g/L.
The embodiment 4 a kind of preparation and application of the liquid fertilizer that fruit corn yield can be improved
A kind of preparation method for the liquid fertilizer that fruit corn yield can be improved the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. 10 parts of chitosan oligosaccharide, urea 98 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Part, poly- 15 parts of asparagus fern door propylhomoserin, 6 parts of phenylalanine, 148 parts of potassium sulfate, 92 parts of calcium superphosphate, 6 parts of boric acid, calcium gluconate 2
Part, 6 parts of white vitriol obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 130g/L.
The embodiment 5 a kind of preparation and application of the liquid fertilizer that fruit corn yield can be improved
A kind of preparation method for the liquid fertilizer that fruit corn yield can be improved the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. 15 parts of chitosan oligosaccharide, urea 120 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Part, poly- 20 parts of asparagus fern door propylhomoserin, 10 parts of phenylalanine, 168 parts of potassium sulfate, 105 parts of calcium superphosphate, 10 parts of boric acid, gluconic acid
5 parts of calcium, 10 parts of white vitriol obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 155g/L.
The comparative example 1 a kind of preparation and application of the liquid fertilizer that fruit corn yield can be improved
A kind of preparation method for the liquid fertilizer that fruit corn yield can be improved the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S4. 10 parts of chitosan oligosaccharide, urea 130 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Part, poly- 15 parts of asparagus fern door propylhomoserin, 6 parts of phenylalanine, 148 parts of potassium sulfate, 71 parts of calcium superphosphate, 10 parts of boric acid, calcium gluconate 2
Part, 2 parts of white vitriol obtain liquid fertilizer.
The application effect appraisal of the liquid fertilizer of fruit corn yield can be improved:
Nutrient needed for liquid fertilizer can quickly supplement the growth of fruit rattan promotes melon and fruit growth.It is prepared by the present invention to verify
The liquid fertilizer actual effect that large-scale demonstration is applied on fruit corn, applicant's selection are tested on fruit corn
Demonstration.
1 materials and methods
1.1 for examination place 1 be located in Zixing City fruit corn base, 12 mu of testing ground area, soil fertility level compared with
Uniformly.
It is located in Jianning city black waxy corn base for examination place 2,12 mu of testing ground area, soil fertility level is more equal
It is even.
1.2 for studying 1 fruit corn of object, rattan line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
For studying 2 black waxy corn of object, rattan line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
1.3 for examination fertilizer embodiment 2~5 made from fruit corn flower and fruit protecting liquid fertilizer, product forms are liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group: every month, which pours, after plantation applies once for trying fertilizer, 500mL/ mus each, dilutes 200 times.
2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.Each group randomly selects 10 plants of corns, counts the average value of corn flowers and fruits quantity, and measurement is protected
Flower and fruit situation.
2 results and analysis
Influence of 2.1 different disposals to fruit-setting rate
As shown in Table 1, the liquid fertilizer that prepared by the present embodiment 2~5 increases the flower number relative comparison group of fruit corn
66.7%, fruit-setting rate is up to 80.0%, relative comparison group increases 60.0%, and tool increases significantly.However, the liquid fertilizer
The facilitation effect of flower quantity and fruit-setting rate to black waxy corn does not all play substantive effect.As it can be seen that liquid of the invention
Body fertilizer has selectivity to the flower and fruit protecting of different corns, the most significant to the flower and fruit protecting effect of fruit corn.
The fruit-setting rate result of the different corn quality different disposals of table 1
| Processing | Fruit ear flower number/piece | Fruit ear bear fruit number/ | Fruit-setting rate/% |
| Fruit corn experimental group | 20 | 16 | 80.0 |
| Fruit corn control group | 12 | 6 | 50.0 |
| Fruit corn comparative example group | 15 | 10 | 66.7 |
| Black waxy corn experimental group | 15 | 11 | 73.3 |
| Black waxy corn control group | 10 | 6 | 60.0 |
| Black waxy corn comparative example group | 14 | 10 | 71.4 |
The influence of 2.2 pairs of per mu yields
Statistics strain number per acre, random 10 points of samplings, count fruiting number, claim fresh fruit weight, calculates fruiting number, single fresh fruit weight
Average value calculates theoretical per mu yield.
Through calculating, 2000 plants of corns are planted per acre, extract 10 plants of corn fruiting numbers, single fresh fruit counterpoise, fruit ear number and mu
The average value of yield such as table 2.As shown in Table 2, the single fresh fruit weight of the fruit corn of the liquid fertilizer has been used to increase compared to the control group
Add 46.4%, has realized that per mu yield increases nearly three times, effect is considerable.However, fruit retention of the liquid fertilizer to black waxy corn
Rate and per mu yield increase only more small effect.As it can be seen that liquid fertilizer of the invention has the flower and fruit protecting of fruit corn
Very significant effect, to the corn of different cultivars selectivity with higher.
The result situation and per mu yield of the different corn quality different disposals of table 2
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (8)
1. a kind of liquid fertilizer that fruit corn yield can be improved, which is characterized in that the liquid fertilizer is by following material composition:
1000 parts of active liquid fertilizer substrate preparation, 5 ~ 15 parts of chitosan oligosaccharide, 80 ~ 120 parts of urea, poly- 10 ~ 20 parts of asparagus fern door propylhomoserin, phenylpropyl alcohol ammonia
Sour 3 ~ 10 parts, 130 ~ 168 parts of potassium sulfate, 80 ~ 105 parts of calcium superphosphate, 1 ~ 10 part of boric acid, 0.1 ~ 5 part of calcium gluconate, seven water sulphur
1 ~ 10 part of sour zinc；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are
120~165g/L;
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY-
The culture medium of 001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then into filtrate
After sugar is added, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that the liquid fertilizer by
Following material composition: 1000 parts of active liquid fertilizer substrate preparation, 8 ~ 12 parts of chitosan oligosaccharide, 90 ~ 110 parts of urea, poly- asparagus fern door propylhomoserin
12 ~ 18 parts, 5 ~ 8 parts of phenylalanine, 145 ~ 153 parts of potassium sulfate, 90 ~ 100 parts of calcium superphosphate, 3 ~ 8 parts of boric acid, calcium gluconate
0.5 ~ 3 part, 2 ~ 8 parts of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g,
The content of organic matter is 120 ~ 135g/L.
3. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that the liquid fertilizer by
Following material composition: 1000 parts of active liquid fertilizer substrate preparation, 10 parts of chitosan oligosaccharide, 98 parts of urea, poly- 15 parts of asparagus fern door propylhomoserin, benzene
6 parts of alanine, 148 parts of potassium sulfate, 92 parts of calcium superphosphate, 6 parts of boric acid, 2 parts of calcium gluconate, 6 parts of white vitriol；The liquid
Total nitrogen content >=2.5mg/g in body fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are 128 ~ 132g/L.
4. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that in the liquid fertilizer
N, the weight ratio of P, K are 8 ~ 15:5:12 ~ 16, preferably 13:5:14.
5. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that the boric acid, grape
The ratio between Calciofon, white vitriol number are 3:1:3.
6. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that in the liquid spawn
Every milliliter has 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is 165g:250L.
7. a kind of preparation method for the liquid fertilizer that fruit corn yield can be improved, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. the configuration of liquid fertilizer: toward the active liquid fertilizer substrate preparation addition chitosan oligosaccharide, urea, poly- asparagus fern door propylhomoserin,
Phenylalanine, potassium sulfate, calcium superphosphate, boric acid, calcium gluconate, white vitriol obtain the liquid fertilizer.
8. the liquid fertilizer of fruit corn yield can be improved according to claim 1, which is characterized in that it is characterized in that, institute
State its amino-acid nitrogen content >=4.5g/L after active liquid fertilizer substrate preparation is stored 15 days.
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| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
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| CN106431629A (en) * | 2016-08-31 | 2017-02-22 | 广德明凯蔬果种植家庭农场 | Adjusting foliar fertilizer |
| CN106676040A (en) * | 2016-12-16 | 2017-05-17 | 吉林农业大学 | Streptomyces griseoplanus, application thereof and microbial agent |
| CN107353127A (en) * | 2017-08-23 | 2017-11-17 | 佛山推启农业研究院（普通合伙） | A kind of microorganism Water soluble fertilizer for preventing and treating corn fungal disease |
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| CN106676040A (en) * | 2016-12-16 | 2017-05-17 | 吉林农业大学 | Streptomyces griseoplanus, application thereof and microbial agent |
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