CN109761668A - A kind of liquid fertilizer and preparation method thereof promoting winter jujube tree root development - Google Patents
A kind of liquid fertilizer and preparation method thereof promoting winter jujube tree root development Download PDFInfo
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Abstract
The invention discloses a kind of liquid fertilizers and preparation method thereof for promoting winter jujube tree root development.The liquid fertilizer includes 1000 parts of active liquid fertilizer substrate preparation；22 ~ 38 parts of nitrogen, 11 ~ 21 parts of potassium, 1 ~ 10 part of phosphorus, calcium 2 ~ 9, magnesium 1 ~ 8,5 ~ 15 parts of boron, 0.1 ~ 8 part of zinc, 0.1 ~ 3 part of molybdenum, 0.1 ~ 3 part of iron；Contain 14 ~ 28 parts of proline, 8 ~ 14 parts of glycine, 8 ~ 19 parts of leucine, 12 ~ 21 parts of methionine, 6 ~ 15 parts of tyrosine, 7 ~ 18 parts of tryptophan, 8 ~ 16 parts of alanine in the liquid fertilizer；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/L；The liquid fertilizer has significant facilitation to winter jujube root amount, Root Distribution and root class group Chengdu.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid for promoting winter jujube tree root development
Body fertilizer and preparation method thereof.
Background technique
Winter jujube is a late-maturing fresh food excellent variety for stingless jujube tree, and the Zizyphus jujuba Mill kind that the quality generally acknowledged is best.
Winter-jujube fruit also contains the various trace elements such as more VitAVitE, potassium, sodium, iron, copper, there is holding capillary
It is unimpeded, prevent the increased function of vascular wall brittleness, it is effective in cure for hypertension, Atheromatosis disease, have the effect of anti-cancer.
Nutritive value is the hat of all kinds of fruits, there is the title of " all kinds of fruits king ".Big, subcircular in fruit, exactly likes griggles, and 14.5 grams of Mean Fruit Weight,
Maximum fruit weighs 46 grams, and uniform in size neat, fruit face is smooth, pericarp crisp fritter, and the white ripe phase is in light green color, after by it is light brown transmit it is reddish brown
Color.Pulp matter is thin, and tender and crisp succulence, dense sweet tea is slightly sour, very palatable, and soluble solid content crisp ripe phase is 34%~38%, complete
It is ripe before up to 40%~42%, quality extremely on.Fruit stone oval, average to weigh 0.6 gram or so, core line is shallow, vertical stripe, kernel-containing rate pole
Height, up to 100%.
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
The root system of winter jujube tree is not only the absorption organ of nutrient and water, and the nutrient situation that can experience soil is produced
Raw chemical signal, and these signals are transmitted to overground part, so that the physiological activity of overground part organ is controlled, as stomata is opened
It closes, the extension of blade, the development of fruit etc..Therefore, a healthy root system is established and keeps, for winter jujube tree overground part
The normal development of stem, leaf, fruit is very important, even more important especially for the seedling just transplanted.Conventional fertilizer application measure
Although plant nutrient can be supplied to, only any nutrient " knowing " needed for root system, is needed how many, when is needed, and
It actively obtains and helps them.Therefore, promote root development be Efficient Cultivation basis.
Summary of the invention
The problem of the technical problem to be solved by the present invention is to overcome existing winter jujube root growths provides a kind of promotion winter jujube tree
Liquid fertilizer of root development and preparation method thereof.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer promoting winter jujube tree root development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；22~38 parts of nitrogen, 11~21 parts of potassium, 1~10 part of phosphorus, calcium 2~9, magnesium 1
~8,5~15 parts of boron, 0.1~8 part of zinc, 0.1~3 part of molybdenum, 0.1~3 part of iron；
Contain 14~28 parts of proline, 8~14 parts of glycine, 8~19 parts of leucine, methionine 12 in the liquid fertilizer
~21 parts, 6~15 parts of tyrosine, 7~18 parts of tryptophan, 8~16 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content
>=0.6mg/g, the content of organic matter >=120g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation.
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, into
Row mashing, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~
7.6。
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
5 building, the building of compound the 59th of state city martyr Road 100.
Further, the liquid fertilizer for promoting winter jujube tree root development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；24~30 parts of nitrogen, 13~16 parts of potassium, 5~8 parts of phosphorus, calcium 3~6, magnesium 2~
5,8~12 parts of boron, 0.5~4 part of zinc, 0.6~2 part of molybdenum, 0.8~2 part of iron；Contain proline 16~22 in the liquid fertilizer
Part, 10~12 parts of glycine, 12~16 parts of leucine, 15~17 parts of methionine, 7~10 parts of tyrosine, 9~12 parts of tryptophan,
11~14 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/L.
Preferably, the liquid fertilizer for promoting winter jujube tree root development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；28 parts of nitrogen, 14 parts of potassium, 7 parts of phosphorus, 6 parts of calcium, 4 parts of magnesium, 10 parts of boron, zinc 2
Part, 0.8 part of molybdenum, 1.1 parts of iron；
Contain 20 parts of proline, 11 parts of glycine, 14 parts of leucine, 16 parts of methionine, tyrosine in the liquid fertilizer
8 parts, 10 parts of tryptophan, 13 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=
120g/L。
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Further, the nitrogen, phosphorus, potassium portion rate be 4:1:2.
Further, the methionine, tyrosine, the ratio of tryptophan are 2:1:1~2.
A kind of preparation method of the liquid fertilizer promoting winter jujube tree root development, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C
After it, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. proline, glycine, leucine, egg the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
One or more of propylhomoserin, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron obtain described
Liquid fertilizer；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
5 building, the building of compound the 59th of state city martyr Road 100.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.1g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
S1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
S2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer of the invention can effectively facilitate the root growth of winter jujube, and be conducive to improve the quality and rush of fruit
Nutrition is absorbed into fruit tree.
Liquid fertilizer of the invention increases 36.25% to total root item number of winter jujube, root amount, Root Distribution and root class composition
All relative comparison groups increase significantly.Compared with the control group, tree height increases 21.5%, and crown diameter increases 42.6%, young sprout
Quantity increases 16.7%, and tree body leaf color is dark green, and chlorophyll content increases obvious.However, rush of the liquid fertilizer to the variety of the Chinese date
Substantive effect is not all played into effect, the Root Distribution and root class composition and Growth status to the variety of the Chinese date only have more small
Effect.Liquid fertilizer of the invention has very significant effect to the strong plantlets and rootage of winter jujube, the life to the jujube tree of different cultivars
Root strong sprout selectivity with higher.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
The present invention is further illustrated below in conjunction with specification and specific embodiment, but embodiment does not appoint the present invention
The restriction of what form.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, side
Method and equipment.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, 220 revs/min on oscillation shaking table, 25 DEG C of conditions
Lower culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is Xianlie Mid Road, Guangzhou City, Guangdong Province, China 100
Number 5 building, the building of compound the 59th.
The embodiment 2 a kind of preparation and application of the liquid fertilizer for promoting winter jujube tree root development
It is a kind of promote winter jujube tree root development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron obtain the liquid
Body fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
The liquid fertilizer of winter jujube tree root development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；28 parts of nitrogen, 14 parts of potassium, 7 parts of phosphorus, 6 parts of calcium, 4 parts of magnesium, 10 parts of boron, zinc 2
Part, 0.8 part of molybdenum, 1.1 parts of iron；In liquid fertilizer containing 20 parts of proline, 11 parts of glycine, 14 parts of leucine, 16 parts of methionine,
8 parts of tyrosine, 10 parts of tryptophan, 13 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, organic matter
Content >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe are respectively derived from ammonium dihydrogen phosphate and amino acid and molybdic acid
Ammonium, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The embodiment 3 a kind of preparation and application of the liquid fertilizer for promoting winter jujube tree root development
It is a kind of promote winter jujube tree root development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g dregs of beans to carry out pre fermentation, being put into fiberizer and be beaten, filter, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
Pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 4 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material
At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days.
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron obtain the liquid
Body fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
The liquid fertilizer of winter jujube tree root development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；24 parts of nitrogen, 12 parts of potassium, 6 parts of phosphorus, 3 parts of calcium, 2 parts of magnesium, 8 parts of boron, zinc 0.5
Part, 0.6 part of molybdenum, 0.8 part of iron；In liquid fertilizer containing 16 parts of proline, 10 parts of glycine, 12 parts of leucine, 15 parts of methionine,
7.5 parts of tyrosine, 9 parts of tryptophan, 11 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g are organic
Matter content >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe are respectively derived from ammonium dihydrogen phosphate and amino acid and molybdenum
Sour ammonium, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The embodiment 4 a kind of preparation and application of the liquid fertilizer for promoting winter jujube tree root development
It is a kind of promote winter jujube tree root development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain Liquid Fertilizer
Material；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
The liquid fertilizer of winter jujube tree root development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；24 parts of nitrogen, 12 parts of potassium, 6 parts of phosphorus, 2 parts of calcium, 1 part of magnesium, 5 parts of boron, zinc 1
Part, 0.1 part of molybdenum, 0.1 part of iron；Contain 14 parts of proline, 8 parts of glycine, 8 parts of leucine, methionine 12 in the liquid fertilizer
Part, 6 parts of tyrosine, 7 parts of tryptophan, 8 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g are organic
Matter content 120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe are respectively derived from ammonium dihydrogen phosphate and amino acid and molybdic acid
Ammonium, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The embodiment 5 a kind of preparation and application of the liquid fertilizer for promoting winter jujube tree root development
It is a kind of promote winter jujube tree root development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain Liquid Fertilizer
Material；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
The liquid fertilizer of winter jujube tree root development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；36 parts of nitrogen, 18 parts of potassium, 9 parts of phosphorus, 9 parts of calcium, 8 parts of magnesium, 15 parts of boron, zinc 8
Part, 3 parts of molybdenum, 3 parts of iron；In the liquid fertilizer containing 28 parts of proline, 14 parts of glycine, 19 parts of leucine, 20 parts of methionine,
10 parts of tyrosine, 18 parts of tryptophan, 16 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g are organic
Matter content 140g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe are respectively derived from ammonium dihydrogen phosphate and amino acid and molybdic acid
Ammonium, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The comparative example 1 a kind of preparation and application of the liquid fertilizer for promoting winter jujube tree root development
It is a kind of promote winter jujube tree root development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation.
S4. proline, glycine, leucine, egg the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
One or more of propylhomoserin, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron obtain described
Liquid fertilizer；
The liquid fertilizer of winter jujube tree root development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；22 parts of nitrogen, 11 parts of potassium, 10 parts of phosphorus, 5 parts of calcium, 5 parts of magnesium, 10 parts of boron, zinc 5
Part, 1 part of molybdenum, 1 part of iron；In the liquid fertilizer containing 20 parts of proline, 10 parts of glycine, 12 parts of leucine, 20 parts of methionine,
15 parts of tyrosine, 7 parts of tryptophan, 16 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, organic matter
Content 130g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe be respectively derived from ammonium dihydrogen phosphate and amino acid and ammonium molybdate,
Ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
Promote the application effect appraisal of the liquid fertilizer of winter jujube tree root development:
Nutrient needed for liquid fertilizer can quickly supplement fruit tree growth promotes winter jujube tree root Stem nematode.For the verifying present invention
The liquid fertilizer of the preparation actual effect that large-scale demonstration is applied on winter jujube fruit tree, applicant's selection are tried in winter jujube
Test demonstration.
1 materials and methods
1.1 are located in Zixing City winter jujube base for examination place 1, and 12 mu of testing ground area, soil fertility level is more uniform.
It is located in Jianning city variety of the Chinese date base for examination place 2,12 mu of testing ground area, soil fertility level is more uniform.
1.2 for studying 1 winter jujube of object, First Year after transplanting, seeding row spacing 4m*4m, the growing way golden mean of the Confucian school and consistent.
For studying 2 variety of the Chinese date of object, First Year after transplanting, seeding row spacing 4m*4m, the growing way golden mean of the Confucian school and consistent.
1.3 for examination fertilizer embodiment 2~5 made from winter jujube tree root development liquid fertilizer, product forms are liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group: it pours within every 3 months after transplanting and applies once for trying fertilizer, it is 500mL/ mus each, dilute 200 times.
2. control group (CK).Per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.After transplanting 1 year it is expired when, each processing randomly selects 5 plants of jujube trees, and observation tree body is raw
Long situation, root amount and root class composition, acquire average value.
2 results and analysis
Influence of 2.1 different disposals to root system
As shown in Table 1, the liquid fertilizer that prepared by the present embodiment 2~5 increases 36.25% to total root item number of winter jujube, root
Amount, Root Distribution and root class group Chengdu relative comparison group increase significantly.However, the liquid fertilizer to the root amount of the variety of the Chinese date,
Substantive effect is not played in Root Distribution and root class group Chengdu.As it can be seen that liquid fertilizer of the invention takes root to different jujube trees
Strong sprout has selectivity, the most significant to the strong plantlets and rootage effect of winter jujube.
Table 1
The influence of 2.2 pairs of trunk infusions growth
As shown in Table 2, which has good facilitation to the trunk infusion growth of winter jujube, is conducive to tree body and supports
The accumulation divided.Wherein compared with the control group, winter jujube tree height increases 21.5%, and crown diameter increases 42.6%, and young sprout quantity increases
16.7%, tree body leaf color is dark green, and chlorophyll content increases obvious.However, the liquid fertilizer to the plant strain growth of the variety of the Chinese date only
Play the role of more small.As it can be seen that liquid fertilizer of the invention has very significant effect to the strong plantlets and rootage of winter jujube, to difference
The jujube tree of kind selectivity with higher.
Table 2
Claims (10)
1. a kind of liquid fertilizer for promoting winter jujube tree root development, which is characterized in that by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；22~38 parts of nitrogen, 11~21 parts of potassium, 1~10 part of phosphorus, calcium 2~9, magnesium 1~8, boron
5~15 parts, 0.1~8 part of zinc, 0.1~3 part of molybdenum, 0.1~3 part of iron；
Contain 14~28 parts of proline, 8~14 parts of glycine, 8~19 parts of leucine, methionine 12~21 in the liquid fertilizer
Part, 6~15 parts of tyrosine, 7~18 parts of tryptophan, 8~16 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=
0.6mg/g, the content of organic matter >=120g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, beaten
Slurry, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
2. promoting the liquid fertilizer of winter jujube tree root development according to claim 1, which is characterized in that by following parts by weight
Material composition:
1000 parts of active liquid fertilizer substrate preparation；24~30 parts of nitrogen, 13~16 parts of potassium, 5~8 parts of phosphorus, calcium 3~6, magnesium 2~5, boron 8
~12 parts, 0.5~4 part of zinc, 0.6~2 part of molybdenum, 0.8~2 part of iron；
In the liquid fertilizer containing 16~22 parts of proline, 10~12 parts of glycine, 12~16 parts of leucine, methionine 15~
17 parts, 7~10 parts of tyrosine, 9~12 parts of tryptophan, 11~14 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content
>=0.6mg/g, the content of organic matter >=120g/L.
3. promoting the liquid fertilizer of winter jujube tree root development according to claim 1, which is characterized in that by following parts by weight
Material composition:
1000 parts of active liquid fertilizer substrate preparation；28 parts of nitrogen, 14 parts of potassium, 7 parts of phosphorus, 6 parts of calcium, 4 parts of magnesium, 10 parts of boron, 2 parts of zinc, molybdenum
0.8 part, 1.1 parts of iron；
In the liquid fertilizer containing 20 parts of proline, 11 parts of glycine, 14 parts of leucine, 16 parts of methionine, 8 parts of tyrosine,
10 parts of tryptophan, 13 parts of alanine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/
L。
4. promoting the liquid fertilizer of winter jujube tree root development according to claim 1, which is characterized in that the pre-fermented step
Suddenly are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans clenching of the hand reaches the state for dripping into line；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material
At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
5. promoting the liquid fertilizer of winter jujube tree root development according to claim 1, which is characterized in that the nitrogen, phosphorus, potassium
Portion rate is 4:1:2.
6. promoting the liquid fertilizer of winter jujube tree root development according to claim 1, which is characterized in that the methionine, network
Propylhomoserin, tryptophan ratio be 2:1:1~2.
7. a kind of preparation method of the liquid fertilizer for promoting winter jujube tree root development, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filtrate
After middle addition sugar, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20~28 DEG C after continuing fermentation 7~8 days,
Obtain fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron obtain the liquid
Body fertilizer；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
5 building, the building of compound the 59th of martyr Road 100.
8. promoting the preparation method of the liquid fertilizer of winter jujube tree root development according to claim 7, which is characterized in that described
Its amino-acid nitrogen content >=4.1g/L after active liquid fertilizer substrate preparation is stored 15 days.
9. promoting the preparation method of the liquid fertilizer of winter jujube tree root development according to claim 7, which is characterized in that described
There are 200,000,000 viable bacterias for every milliliter in liquid spawn；The ratio of the liquid spawn and culture medium is 165g:250L.
10. any one of claim 1 to 9 liquid fertilizer the answering in winter jujube cultivation for promoting winter jujube tree root development
With.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN104098399A (en) * | 2014-07-24 | 2014-10-15 | 句容市天王镇戴庄有机农业专业合作社 | Special fruit tree liquid fertilizer and preparation method thereof |
| CN106495826A (en) * | 2016-09-23 | 2017-03-15 | 深圳市三盛环保科技有限公司 | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer |
-
2017
- 2017-11-09 CN CN201711097075.6A patent/CN109761668A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN104098399A (en) * | 2014-07-24 | 2014-10-15 | 句容市天王镇戴庄有机农业专业合作社 | Special fruit tree liquid fertilizer and preparation method thereof |
| CN106495826A (en) * | 2016-09-23 | 2017-03-15 | 深圳市三盛环保科技有限公司 | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer |
Non-Patent Citations (1)
| Title |
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| 李颖等: "《现代微生物研究技术》", 31 October 2008, 中国农业大学出版社 * |
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