CN109956837A - A kind of how anti-compound fertilizer of tea seed cake and its preparation method and application - Google Patents
A kind of how anti-compound fertilizer of tea seed cake and its preparation method and application Download PDFInfo
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- CN109956837A CN109956837A CN201711427633.0A CN201711427633A CN109956837A CN 109956837 A CN109956837 A CN 109956837A CN 201711427633 A CN201711427633 A CN 201711427633A CN 109956837 A CN109956837 A CN 109956837A
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- tea seed
- seed cake
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
Abstract
The invention discloses how anti-compound fertilizers of a kind of tea seed cake and its preparation method and application.The how anti-compound fertilizer of the tea seed cake is made of compound fertilizer with the material mixing containing organic matter, and the total content of the organic matter is 28 ~ 33%, and the total content of N, P, K are 11~15%；The substance containing organic matter is tea seed cake, humic acid, microbial strain culture, amino acid；The how anti-compound fertilizer of the tea seed cake is particularly suitable for base manure or the top dressing of the crops such as field crop and various gourd, fruit and vegetables, it is all greatly improved using the nutritional benefit element in the yield and quality and fruit of rear field crop and various gourd, fruit and vegetables, unexpected technical effect is achieved to the growth and development of field crop and various gourd, fruit and vegetables.
Description
Technical field
The present invention relates to the technical fields of compound fertilizer, more particularly, to a kind of how anti-compound fertilizer of tea seed cake and its preparation
Methods and applications.
Background technique
Chemical fertilizer is because cost is relatively low, efficiency is higher by universal use.But application chemical fertilizer can not only make
The physico chemical factor of soil degenerates, and hinders the breeding of septic biology in soil, threaten their existence, disorder soil
The ecosystem, consequence are that harmful parasitic organism is easy actively, to improve their population densities, to be then easy to more
Morbidity insect pest, and earth is made to pull knot, soil fertility reduces.
Although urea is organic matter, but it is a kind of nitrogenous fertilizer, and ingredient is single.Urea water after edaphon acts on
Solution belongs to quick-acting fertilizer at a kind of ingredient of ammonium carbonate.Urea will not bring organic matter to soil, will not promote microbial reproduction,
The physicochemical property and bioactivity that soil will not be improved, can reduce soil physico-chemical property and bioactivity, make soil after a large amount of uses
Earth is hardened.
The breeding of beneficial bacterium in soil can be effectively facilitated by applying high-quality organic matter fertilizer, improved, provided or promote in soil
Beneficial bacterium generates nutrient necessary to crops, plays an important role to crop development, and rises to the ecosystem in soil
To good improvement result.
But in existing organic fertilizer, if livestock and poultry manure is often with more various viruses, parasitic ovum, evil
Stink etc., some organic fertilizers such as weeds etc. are often with various pest and disease damage infectious agents and seed etc., so general organic fertilizer is such as
Fruit can all be labeled with greatly discount without scientific processing, the good result of final organic fertilizer.Organic matter type is more numerous
It is more, between the different growing stages of different plants or same plant, the content of organic matter of the organic fertilizer needed,
The type of nutrient is different；And in existing general organic fertilizer compatibility, it is also possible to contain certain for being not suitable for certain plant
A little ingredients, such as sea fertilizer etc. are often with higher to the harmful substance of growth of tea plant (such as chloride ion).Therefore, to realize certain
The specific plantation of kind and cultivation purpose, the organic fertilizer for summarizing to obtain specific composition for Journal of Sex Research have great importance.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the technical deficiencies of the how anti-compound fertilizer of existing tea seed cake, provide a kind of tea seed cake
Mostly anti-compound fertilizer.
Another technical problems to be solved of the present invention are to provide the preparation method of the how anti-compound fertilizer of the tea seed cake.
There are one the applications that the technical issues that need to address are to provide the how anti-compound fertilizer of the tea seed cake by the present invention.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of how anti-compound fertilizer of tea seed cake is provided, is made of compound fertilizer and the material mixing containing organic matter, in the compound fertilizer
The total content of organic matter is 28~33%, and the total content of N, P, K are 11~15%；The substance containing organic matter is by tea seed cake, corruption
Phytic acid, microbial strain culture, amino acid mix；
The microbial strain culture is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY-001# "
Culture medium are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then sugar is added into filtrate
Afterwards, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Preferably, the humic acid uses one of lignite weathering humic acid, fulvic acid, ulmic acid, humic acid；
Preferably, according to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
Preferably, according to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
Preferably, the compound fertilizer be containing urea, Diammonium phosphate (DAP), potassium sulfate chemical fertilizer, containing N, P, K total content be 8.7%.
Invention also provides the preparation methods of the how anti-compound fertilizer of the tea seed cake, comprising the following steps:
S1. it is fermented to obtain tea seed cake fermentation material after bio-fermentation agent being added into tea seed cake；Humic acid crushed
Sieve, obtains crushed material；Prepare microbial strain culture；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, adjust pH value after again with compound fertilizer, amino
Acid-mixed is closed, and is packed or is packed after being granulated to obtain the final product.
The bio-fermentation agent is aspergillus niger microbial inoculum, bacillus subtilis, lactobacillus plantarum, one in EM microbial bacterial agent
Kind.The how anti-compound fertilizer of tea seed cake is graininess or powdery.
Preferably, the moisture content of tea seed cake described in step S1 is adjusted to 40~50% or so；
Preferably, the additional amount of bio-fermentation agent described in step S1 is according to tea seed cake: the portion rate of bio-fermentation agent be 10~
20:1 is determined.
Preferably, the process conditions of fermentation described in step S1 are as follows: fermentation temperature is 55~60 DEG C, fermentation time is 10~14
It, every turning in 4~7 days is primary.
Preferably, humic acid powder was broken to partial size described in step S1 is 30~100 meshes.
Preferably, described in step S2 tune pH value be adjusted to pH value be 5.5~8；Further, it is preferable to using technical hydrochloric acid
Or quick lime is adjusted to pH value；It is preferred that use mass percent concentration for 5~10% sulphur acid for adjusting pH value.
It is further preferred that the preparation method of the microbial strain culture the following steps are included:
S01. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then
After sugar is added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S02. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S03. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C
After it, fermentation liquid is obtained；
S04. it terminates fermentation: fermentation liquid loading sealing is stood 15 days to get microbial strain culture.
Further, aforementioned pre-fermented step are as follows:
S011. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S012. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S013. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
The how anti-compound fertilizer of tea seed cake prepared by the present invention is rich in the more resistant activity nutrients of tea seed cake, amino acid, Tea Saponin, can promote
Grain Growth Situation is healthy and strong, and enhancing crop cold resistance, resistant to lodging, anti-continuous cropping, saline-alkali tolerance energy are conducive to prevent and treat underground pests, except low
Pesticide investment, improves commodity value and competitive advantage.
A small amount of organic matter combined humic acid can effectively improve soil, abolish hardened, improve Water and fertilizer retention ability of soil, make soil
Ground the more is planted the more fertile, high crop yield stable yields；It retains and conserve soil moisture, dissolving phosphor and dissolving potassium function stimulates crop root growth, blocks soil-borne disease；
Microelement is able to satisfy the needs of healthy growth of crops and good quality and high output in N P and K abundant, effectively prevents and bright
The aobvious generation for reducing physiological disturbance realizes field crop and various gourd, fruit and vegetable products is high-quality, high yield.
Compound fertilizer prepared by the present invention combines organic-inorganic, and utilization rate of fertilizer is high, reduces agriculture production cost, improves agriculture
People's income；Fertilizer property is stablized, and few, pollution of the reduction fertilising to environment is lost.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The preferred preparation of 1 microbial strain culture of embodiment
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.6.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.6.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 30cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.6.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
Microbial strain culture the preparation method is as follows:
S01. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；
Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH 7.6；
Pre-fermented step are as follows:
S011. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S012. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S013. the pre fermentation time is 4 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, inside fermentation raw material
When temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days.
S02. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium obtained by step S01,
It stirs evenly；There are 200,000,000 viable bacterias for every milliliter in liquid spawn；The ratio of liquid spawn and culture medium is 165g:250L
S03. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid (microbial strain culture) is obtained.
The preparation of the how anti-compound fertilizer of 2 tea seed cake of embodiment
According to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
The how anti-compound fertilizer of tea seed cake is prepared by following preparation step:
S1. it after containing EM microbial bacterial agent is added in tea seed cake, ferments 10 days, turns under the conditions of being 55 DEG C in fermentation temperature within 5 days
Heap is primary, obtains tea seed cake fermentation material；Microbial strain culture is prepared by the method and step in embodiment 1；Humic acid is subjected to powder
Being broken to partial size is 30 meshes, obtains crushed material；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, using 5% sulfuric acid tune pH value to 6.5
It mixes, packs after granulation up to the how anti-compound fertilizer of tea seed cake with compound fertilizer, amino acid again afterwards.
The preparation of the how anti-compound fertilizer of 3 tea seed cake of embodiment
According to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
The how anti-compound fertilizer of tea seed cake is prepared by following preparation step:
S1. it after containing EM microbial bacterial agent is added in tea seed cake, ferments 14 days, turns under the conditions of being 55 DEG C in fermentation temperature within 7 days
Heap is primary, obtains tea seed cake fermentation material；Microbial strain culture is prepared by the method and step in embodiment 1；Humic acid is subjected to powder
Being broken to partial size is 30 meshes, obtains crushed material；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, using 5% sulfuric acid tune pH value to 6.5
It mixes, packs after granulation up to the how anti-compound fertilizer of tea seed cake with compound fertilizer, amino acid again afterwards.
The preparation of the how anti-compound fertilizer of 4 tea seed cake of embodiment
According to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
The how anti-compound fertilizer of tea seed cake is prepared by following preparation step:
S1. it after containing EM microbial bacterial agent is added in tea seed cake, ferments 10 days, turns under the conditions of being 55 DEG C in fermentation temperature within 5 days
Heap is primary, obtains tea seed cake fermentation material；Microbial strain culture is prepared by the method and step in embodiment 1；Humic acid is subjected to powder
Being broken to partial size is 30 meshes, obtains crushed material；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, using 5% sulfuric acid tune pH value to 6.5
It mixes, packs after granulation up to the how anti-compound fertilizer of tea seed cake with compound fertilizer, amino acid again afterwards.
The preparation of the how anti-compound fertilizer of 5 tea seed cake of embodiment
According to mass fraction meter, the how anti-compound fertilizer of tea seed cake is made of in proportion following each component:
The how anti-compound fertilizer of tea seed cake is prepared by following preparation step:
S1. it after containing EM microbial bacterial agent is added in tea seed cake, ferments 10 days, turns under the conditions of being 55 DEG C in fermentation temperature within 5 days
Heap is primary, obtains tea seed cake fermentation material；Microbial strain culture is prepared by the method and step in embodiment 1；Humic acid is subjected to powder
Being broken to partial size is 30 meshes, obtains crushed material；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, using 5% sulfuric acid tune pH value to 6.5
It mixes, packs after granulation up to the how anti-compound fertilizer of tea seed cake with compound fertilizer, amino acid again afterwards.
6 application test of embodiment
1. promoting the application effect appraisal of field crop and various gourd, fruit and vegetables:
Nutrient needed for fertilizer of the present invention can quickly supplement field crop and the growth of various gourd, fruit and vegetables, promotes field crop
And various gourd, fruit and vegetable growths.To verify fertilizer large-scale demonstration on field crop and various gourd, fruit and vegetables prepared by the present invention
The actual effect of application, applicant's selection have carried out experiment and demonstration on corn.
1 materials and methods
1.1 are located in Zixing City corn base for examination place 1, and 10 mu of testing ground area, soil fertility level is more uniform.
1.2 for studying 1 corn of object, plant line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
1.3 mixed organic manure for trying to improve corn fruit quality made from fertilizer embodiment 2~5, product forms are powder
Shape or particle.
1.4 carry out each 2 mu of each processing group of 5 test process for examination place:
1. 2 groups of embodiment: soil is irrigated into bottom water, by embodiment 2 prepare for examination fertilizer with 80kg/ it is per mu it is even apply,
Hereafter fertilising in every 15 days is primary, applies 5kg urea and 5kg potassium sulfate per acre and applies to water leaching.
2. 3 groups of embodiment: soil is irrigated into bottom water, by embodiment 3 prepare for examination fertilizer with 80kg/ it is per mu it is even apply,
Hereafter fertilising in every 15 days is primary, applies 5kg urea and 5kg potassium sulfate per acre and applies to water leaching.
3. 4 groups of embodiment: soil is irrigated into bottom water, by embodiment 4 prepare for examination fertilizer with 80kg/ it is per mu it is even apply,
Hereafter fertilising in every 15 days is primary, applies 5kg urea and 5kg potassium sulfate per acre and applies to water leaching.
4. 5 groups of embodiment: soil is irrigated into bottom water, by embodiment 5 prepare for examination fertilizer with 80kg/ it is per mu it is even apply,
Hereafter fertilising in every 15 days is primary, applies 5kg urea and 5kg potassium sulfate per acre and applies to water leaching.
5. control group (CK): being replaced every time with the similar outer production fertilizer of same experimental group equivalent for trying fertilizer (Sichuan Lei Nanong per acre
Crop composite chemical fertilizer) same time progress, the same processing of other measures of fertilizer is 1..
Embodiment group and control group are managed by local conventional cultivation technology.Each processing is tested with per acre for cell,
Each processing group randomly selects 10 plants of corn measurement growth and development states, repeats to choose 3 times, acquires average value.
2 results and analysis
The influence of 2.1 pairs of fruits nutrition substances
As shown in Table 1, protein highest content in the corn of the mixed organic manure is applied to increase compared to the control group
39.7%, amino acid highest content increases 52.9% compared to the control group, and starch highest content increases compared to the control group
23.3%, highest sugar content increases 37.5% compared to the control group, and highest fat content increases 67.7% compared to the control group.
Table 1
The influence of 2.2 pairs of per mu yields
Statistics strain number per acre, random 10 points of samplings, count fruiting number, claim fresh fruit weight, calculates fruiting number, single fresh fruit weight
Average value calculates theoretical per mu yield, as shown in table 2.
Through calculating, 2000 plants of corns are planted per acre, extract 10 plants of corn fruiting numbers, single fresh fruit counterpoise, fruit ear number and mu
The average value of yield such as table 2.As shown in Table 2, the single fresh fruit weight of the corn of the mixed organic manure has been used to increase compared to the control group
About 66.7%, realize that per mu yield increases about 80%, effect is considerable.
Table 2
| Processing | Fruit ear fruiting number/ | Fresh fruit counterpoise/kg | Per mu yield/kg |
| 2 groups of embodiment | 12 | 0.35 | 840 |
| 3 groups of embodiment | 16 | 0.41 | 1312 |
| 4 groups of embodiment | 13 | 0.37 | 962 |
| 5 groups of embodiment | 15 | 0.39 | 1170 |
| Control group | 9 | 0.32 | 576 |
The influence of 2.3 pairs of soluble solid contents
It takes several fresh fruit fruits to squeeze the juice at random, measures soluble fixture content, experimental group and control group respectively measure 5
It is secondary, it is averaged.Experimental result is as shown in table 3.
As shown in Table 3, which improves a lot to the fruit quality of corn, soluble solid content phase
25% is increased for control group.
Table 3
| Processing | Soluble solid content/% |
| 2 groups of embodiment | 14.2 |
| 3 groups of embodiment | 15.5 |
| 4 groups of embodiment | 14.6 |
| 5 groups of embodiment | 14.9 |
| Control group | 12.2 |
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (10)
1. a kind of how anti-compound fertilizer of tea seed cake, which is characterized in that be made of compound fertilizer and the material mixing containing organic matter, the compound
The total content of organic matter is 28 ~ 33% in fertilizer, and the total content of N, P, K are 11~15%；The substance containing organic matter is by tea seed cake, corruption
Phytic acid, microbial strain culture and amino acid mix；
The microbial strain culture is obtained by streptomyces bacterial strain " TY-001# " fermentation；The culture of streptomyces bacterial strain " TY-001# "
Base are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then after sugar being added into filtrate, add
Water is settled to concentration >=100g/L sugared in culture medium, and pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. the how anti-compound fertilizer of tea seed cake according to claim 1, which is characterized in that the humic acid uses lignite weathering humic
One of acid, fulvic acid, ulmic acid, humic acid.
3. the how anti-compound fertilizer of tea seed cake according to claim 2, which is characterized in that according to mass fraction meter, the tea seed cake is more
Anti- compound fertilizer is made of in proportion following each component:
10 ~ 14 parts of compound fertilizer；
3 ~ 8 parts of amino acid；
1 ~ 5 part of tea seed cake；
12 ~ 18 parts of humic acid；
23 ~ 28 parts of microbial strain culture.
4. the how anti-compound fertilizer of tea seed cake according to claim 1, which is characterized in that according to mass fraction meter, the tea seed cake is more
Anti- compound fertilizer is made of in proportion following each component:
12 parts of compound fertilizer；
5 parts of amino acid；
4 parts of tea seed cake；
15 parts of humic acid；
26 parts of microbial strain culture.
5. the how anti-compound fertilizer of tea seed cake according to claim 1, which is characterized in that the compound fertilizer is containing urea, di(2-ethylhexyl)phosphate
The chemical fertilizer of ammonium, potassium sulfate is 8.7% containing N, P, K total content.
6. the preparation method of any one of claim 1 to the 5 how anti-compound fertilizer of tea seed cake, which comprises the following steps:
S1. it is fermented to obtain tea seed cake fermentation material after bio-fermentation agent being added into tea seed cake；Humic acid is pulverized and sieved, is obtained
To crushed material；Prepare microbial strain culture；
S2. tea seed cake fermentation material, microbial strain culture and crushed material are mixed in proportion, adjust pH value after again with compound fertilizer, amino acid-mixed
It closes, packs or packed after being granulated to obtain the final product.
7. the how anti-compound fertilizer of tea seed cake according to claim 6, which is characterized in that be broken to humic acid powder described in step S1
Partial size is 30 ~ 100 meshes.
8. the how anti-compound fertilizer of tea seed cake according to claim 1, which is characterized in that the bio-fermentation agent is black-koji mould
One of agent, bacillus subtilis, lactobacillus plantarum, EM microbial bacterial agent.
9. the how anti-compound fertilizer of tea seed cake according to claim 1, which is characterized in that the microbial strain culture
Preparation method the following steps are included:
S01. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S02. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S03. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S04. it terminates fermentation: fermentation liquid loading sealing is stood 15 days to get microbial strain culture.
10. the application of any one of claim 1 to the 9 how anti-compound fertilizer of tea seed cake, which is characterized in that be applied to field crop and
The base fertilizer of various gourd, fruit and vegetables or top dressing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112939674A (en) * | 2021-04-02 | 2021-06-11 | 江西省农业科学院土壤肥料与资源环境研究所 | Special fertilizer for cash crops and preparation method thereof |
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
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| CN103030477A (en) * | 2012-12-24 | 2013-04-10 | 冷登书 | Nutrient soil special for desertification control and preparation method thereof |
| CN103288554A (en) * | 2013-06-27 | 2013-09-11 | 苏州禾润农业科技有限公司 | Organic microbial fertilizer for lettuces and preparation method thereof |
| CN103787746A (en) * | 2014-01-10 | 2014-05-14 | 武汉盛大神农生物工程有限公司 | Preparation for reducing soil-borne disease, promoting rooting and resisting continuous cropping |
| CN103964946A (en) * | 2014-04-18 | 2014-08-06 | 湖南泰谷生物科技股份有限公司 | Special compound microbial fertilizer for paddy rice and preparation method thereof |
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| CN101456777A (en) * | 2008-12-30 | 2009-06-17 | 仲元(北京)绿色生物技术开发有限公司 | Soil fertility amendment and preparation method thereof |
| CN103030477A (en) * | 2012-12-24 | 2013-04-10 | 冷登书 | Nutrient soil special for desertification control and preparation method thereof |
| CN103288554A (en) * | 2013-06-27 | 2013-09-11 | 苏州禾润农业科技有限公司 | Organic microbial fertilizer for lettuces and preparation method thereof |
| CN103787746A (en) * | 2014-01-10 | 2014-05-14 | 武汉盛大神农生物工程有限公司 | Preparation for reducing soil-borne disease, promoting rooting and resisting continuous cropping |
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| CN112939674A (en) * | 2021-04-02 | 2021-06-11 | 江西省农业科学院土壤肥料与资源环境研究所 | Special fertilizer for cash crops and preparation method thereof |
| CN114436705A (en) * | 2022-03-18 | 2022-05-06 | 河南省驻科生物科技有限公司 | Liquid fertilizer for increasing yield and preventing and treating plant diseases and insect pests and preparation method thereof |
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