CN107935788A - A kind of soil remediation biofermentation fertilizer - Google Patents
A kind of soil remediation biofermentation fertilizer Download PDFInfo
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- CN107935788A CN107935788A CN201711497820.6A CN201711497820A CN107935788A CN 107935788 A CN107935788 A CN 107935788A CN 201711497820 A CN201711497820 A CN 201711497820A CN 107935788 A CN107935788 A CN 107935788A
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- soil remediation
- fertilizer
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- biofermentation
- soil
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention discloses a kind of soil remediation biofermentation fertilizer, the soil remediation biofermentation fertilizer is by dipotassium hydrogen phosphate, urea and material composition containing organic matter, and the total content of organic matter is >=44% in the biofermentation fertilizer, N P and K total content >=17.1%；The material containing organic matter is pumpkin, humic acid, microbial strain culture, beans root powder and plaster；The plaster is clay, calcium and magnesium carbonate and the natural mixture of shell residue.The present invention improves soil ecology balance using microorganism fungus kind culture and the multiple-microorganism bacterium fixed nitrogen in legume, adjusting acid soil while supplying crop nutrition.
Description
Technical field
The present invention relates to the technical field of fertilizer, more particularly, to a kind of soil remediation biofermentation fertilizer.
Background technology
Soil acidification is a kind of form of expression caused by the imbalance of soil rational structure, and soil acidity increase causes pH value to drop
Low formation acid soil, highly acid, a kind of extremely strong acid balance phenomenon.The pH value of soil declines, it is caused more than
Disease and pest will hinder the growth of plant.And strong acid environment can also accelerate toxic metals filtering out and permeate to surrounding water
Enter in underground water and flee hither and thither, endanger environment and human health.
The acid-base value of soil is vital to plant growth, and most of crops all can be in neutral or subacidity soil
Grow vigorously, but in order to devote exclusive attention to output, traditional agriculture liming, the excrement that makes a fire, apply family's fertilizer etc. small missing it is chronic accumulative, cause
Arable soil rational structure is unbalance.Since the planting technology of chemical fertilizer too relies on, long-term chemical fertilizer of largely applying is even more to cause soil
The one of the major reasons of hardened acidifying, and cause Chinese soil to be acidified hardened arch-criminal.
The breeding of beneficial bacterium in soil can be effectively facilitated using high-quality organic matter fertilizer, is improved, there is provided or promote in soil
Nutrient necessary to beneficial bacterium generation crops, plays an important role crop development, and the ecosystem in soil is risen
To good improvement result.
But in existing organic biological ferment fertilizer, if livestock and poultry manure is often with more various viruses, parasitism
Worm's ovum, foul smell etc., some organic biological ferments fertilizer such as weeds often with various pest and disease damage infectious agents and seed etc., so
If general organic biological ferment fertilizer is not only unable to rehabilitating soil acid-base balance, can also reduce fruit without the processing of science
The resistance of vegetable.
The content of the invention
The technical problem to be solved in the present invention is the technical deficiency for overcoming existing biofermentation fertilizer, there is provided a kind of soil remediation
Biofermentation fertilizer.
Another technical problems to be solved of the present invention are to provide the preparation method of the soil remediation biofermentation fertilizer.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
A kind of soil remediation biofermentation fertilizer is provided, it is described by dipotassium hydrogen phosphate, urea and material composition containing organic matter
The total content of organic matter is >=44% in biofermentation fertilizer, N P and K total content >=17.1%；The material containing organic matter is
Pumpkin, humic acid, microbial strain culture, beans root powder and plaster；
The microbial strain culture is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY-001# "
Culture medium is：After taking soybean to be soaked or taking dregs of beans to carry out pre fermentation, it is beaten, is filtered；Then toward addition sugar in filtrate
Afterwards, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, Classification And Nomenclature Streptomyces., its deposit number are GDMCC No.：60152, preservation address is that Guangdong province, China is wide
Five building, Institute of Micro-biology of the province laboratory building of state city martyr Road 100.
Preferably, the plaster is clay, calcium and magnesium carbonate and the natural mixture of shell residue.
Preferably, after the beans root powder plucks fruit by legume, remaining, stem, leaf, beanpod crush after drying
Arrive.
Preferably, the humic acid uses lignite weathering humic acid.
Preferably, calculated according to organic matter total content in fermentation fertilizer, the content of organic matter >=1.2% in the beans root powder, institute
State organic matter total content >=24% in microbial strain culture, organic matter total content >=3% in the humic acid.
Preferably, it is made of in proportion following each component according to mass fraction meter, the soil remediation biofermentation fertilizer：
Preferably, it is made of in proportion following each component according to mass fraction meter, the soil remediation biofermentation fertilizer：
Present invention simultaneously provides the preparation method of the soil remediation biofermentation fertilizer, comprise the following steps：
S1. pumpkin is crushed plus water, high temperature sterilization postcooling, addition bio-fermentation agent is fermented to obtain pumpkin fermenting
Material；Prepare microbial strain culture；Humic acid, beans root powder, plaster are pulverized and sieved, is mixed to get and is pulverized and mixed material；
S2. adjust pH value by pumpkin fermenting material, microbial strain culture and after being pulverized and mixed material mixing, then with urea, phosphoric acid hydrogen two
Potassium mixes in proportion, is granulated up to biofermentation fertilizer.
Preferably, the bio-fermentation agent for lactic acid bacteria, saccharomycete, photosynthetic bacteria, bacillus category, filamentous fungi, plant
One or more in thing lactobacillus, EM microbial bacterial agents.
Preferably, the humic acid powder was broken to 20~30 mesh sieves；Beans root powder was crushed to 10~20 mesh sieves；Plaster crushes
To 10~20 mesh sieves excessively.Preferably, the ratio between the bio-fermentation agent and pumpkin number are 1:15~20.
Preferably, the technological parameter to ferment described in step S1 is：Fermentation temperature is 55~60 DEG C, fermentation time be 10~
14 days, every turning in 4~7 days was once.
Preferably, it is that adjusting to pH value is 5~8 that pH value is adjusted described in step S2；Further, it is preferable to using mass percent
The sulfuric acid or quick lime that concentration is 5~10% adjust pH value.
The soil remediation biofermentation fertilizer is powdery or graininess.
The soil remediation biofermentation fertilizer can be advantageously applied in acid soil as Chinese cabbage, sorghum, wheat etc.
Fruits and vegetables, the base manure of crops or top dressing.
It is further preferred that the preparation method of the microbial strain culture comprises the following steps：
S01. culture medium is prepared：After taking soybean to be soaked or taking dregs of beans to carry out pre fermentation, it is beaten, is filtered；Then
After adding sugar into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S02. it is inoculated with：The liquid spawn of streptomyces bacterial strain " TY-001# " is taken, is inoculated into culture medium, is stirred evenly；
S03. ferment：During the fermentation toward being continually fed into oxygen in culture medium, the continuing fermentation 7~8 at 20~28 DEG C
After it, zymotic fluid is obtained；
S04. fermentation is terminated：Zymotic fluid loading sealing is stood 15 days, up to microbial strain culture.
Further, foregoing pre-fermented step is：
S011. toward in dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
Can；
S012. dregs of beans surface is covered in film, left no gaps, timing starts pre fermentation process；
S013. the pre fermentation time is 3~5 days；After starting fermentation 2 days, by thermometer insertion dregs of beans depths, in fermentation raw material
During portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2~3 days.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprises the following steps：
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
My god, bacterial strain after activation is made；
Y2. inoculation is into fluid nutrient medium after taking the obtained activation of step Y1, temperature be 25~30 DEG C, revolution be
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, obtains bacterium solution.
Preferably, described every liter of component of fluid nutrient medium is as follows：Soybean, dregs of beans 100g is taken to wear into soya-bean milk filtering, waste；In vain
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, described every liter of component of fluid nutrient medium is as follows：Beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, described every liter of component of solid medium is as follows：Beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the advantages that：
The invention by microbial fermentation combination legume, acid soil can be repaired by adding plaster and preparing
Biofermentation fertilizer, plaster can quick of short duration adjusting acid soil, belong to natural materials, therefore safety and environmental protection, and microorganism
Both the microbial strain culture prepared and legume combination of fermenting produce multiple-microorganism bacterium, can persistently improve soil acid-base and put down
Weigh, bacterium is one kind biology that quantity is maximum in soil, its quantity and kind are also maximum, most in rhizosphere circle.It is although native
Many bacteriums have the correction effect of different degree on pollutant in earth, but in rhizosphere circle, because plant root exudation
The supply of thing and other nutriments, its vital movement is unusual active, also extremely obvious to the correction effect of acid soil.At the same time
Microorganism has life, and suitable suitable growing environment, effective guarantee soil ecology balance can be adjusted according to soil regime.
Containing abundant rhizobium in beans root powder, this bacterium can be secreted in the soil around the Dan Daogen areas of surplus, there is provided can
The nitrogen of dissolving is utilized to plant；On the other hand, these rhizobium can obtain energy from plant, and then produce nitrogen fixation.
Legume is all soil conditioner, can provide nutrient, and the nitrogen content of its leaf is higher by 25% than other plants.Soil acidification rises
Because there is the phosphate fertilizer for being not dissolved in water in soil, rhizobium can go out from special soil microorganism and bacteria distribution, have by secretion
Phosphate fertilizer not soluble in water in soil, is transformed into soluble form by machine acid, and thus the acidity of soil can just be reversed.
Rich in abundant organic matter and trace element in biofermentation fertilizer prepared by the present invention, there is more than 95% nitrogen to be with
Organic state is present in soil.In addition, organic matter is also the important next of phosphorus in soil, Calcium Magnesium Sulphur and trace element
Source.So the soil more than organic matter, nutrient content is also just more.Humic acid in organic matter, can strengthen plant respiration, improve thin
Born of the same parents' permeability of the membrane, strengthens the absorption to nutriment, while the vitamin in organic matter and some hormones can promote plant
Growth and development.
The present invention use the fermentation material after pumpkin fermenting as raw material, and the carrotene enriched, amino acid, more is rich in pumpkin
Sugar and various trace elements, abundant nutrient is provided for resistance building, and foster element during to yield positive results, which absorbs, escorts
Escort.The present invention can alleviate the unsalable situation of many places pumpkin, promote the plantation and marketing of pumpkin.
The humic acid of proper ratio is added in fertilizer prepared by the present invention, humus is the main cementing of soil agreegate
Agent, can promote the formation of satisfactory texture, can also increase soil heat absorption capacity, increase soil fertility, and it is elastic to create suitable soil
Degree.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
Support.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
Limit.Unless stated otherwise, the reagent of the invention used, method and apparatus is the art conventional reagent, methods and apparatus.
Unless stated otherwise, following embodiments agents useful for same and material are purchased in market.
The preferred preparation of 1 microbial strain culture of embodiment
1. experiment material and condition
(1) pedotheque collected in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows：Beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
Rise, pH 7.4.
Every liter of component of solid medium is as follows：Beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15g, water are fixed
Hold to 1 liter, pH 7.4.
Culture medium dispenses, high-pressure sterilizing pot sterilizing (121 DEG C, 30min).
(3) aseptic technique：All vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Carried out Deng operation in superclean bench.
(4) strain culturing condition：It is placed in 25 ± 1 DEG C of illumination insulating box (14L：Culture in 10D), after bacterium colony is formed, turns
PDA inclined-planes are moved on to, then is transferred in 5 DEG C of refrigerators and preserves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid mediums, then 5 DEG C of ice are transferred to
Preserved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on vibration shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) cultivate 5 days after T0 liquid bacterias, be positioned over 20 watts of ultraviolet lamp 20cm at irradiate 2 it is small when.
(5) it will be cultivated 5 days in the T0 liquid bacterias access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) some processes all over step (2)~(5) are repeated to circulate.In each circulation all over repetitive process, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and preserves.
(7) the osmophilic strain bacterial strain T0 filtered out by more than, carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in zymotic fluid (by formol titration measure ammonia nitrogen standard detection) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows：Take soybean 100g to wear into soya-bean milk filtering, abandon bean dregs；White granulated sugar
100g, is settled to 1000mL, pH 7.4.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature is：Bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, its deposit number is GDMCC No.：60152, preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Five building, biological institute's laboratory building.
The preparation method of microbial strain culture is as follows：
S01. culture medium is prepared：After taking soybean to be soaked or taking dregs of beans to carry out pre fermentation, it is beaten, mistake
Filter；Then toward after adding sugar in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH
For 7.4~7.6；
Pre-fermented step is：
S011. toward in dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
Can；
S012. dregs of beans surface is covered in film, left no gaps, timing starts pre fermentation process；
S013. the pre fermentation time is 4 days；After starting fermentation 2 days, by thermometer insertion dregs of beans depths, inside fermentation raw material
During temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days.
S02. it is inoculated with：The liquid spawn of streptomyces bacterial strain " TY-001# " is taken, is inoculated into culture medium obtained by step S01,
Stir evenly；There are 200,000,000 viable bacterias for every milliliter in liquid spawn；The ratio of liquid spawn and culture medium is 165g:250L
S03. ferment：Oxygen pump is opened, during the fermentation toward being continually fed into oxygen in fermenter, and according in fermenter
Independent Slurry bubble situation, adds suitable defoamer.Fermenter is covered with ventilated membrane, in order to avoid falling into debris, is held at 25 DEG C
After supervention ferment 7 days, zymotic fluid (microbial strain culture) is obtained.
The preparation of 2 soil remediation biofermentation fertilizer of embodiment
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Beans root powder was crushed to 12 mesh sieves；Plaster, which was crushed to 12 mesh sieves and is mixed to get, is pulverized and mixed material；
S2. use mass percent dense being pulverized and mixed after material mixing after pumpkin fermenting material, microbial strain culture and sieving
Spend and mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value, is granulated up to biofermentation fertilizer.
The preparation of 3 soil remediation biofermentation fertilizer of embodiment
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Beans root powder was crushed to 12 mesh sieves；Plaster, which was crushed to 12 mesh sieves and is mixed to get, is pulverized and mixed material；
S2. use mass percent dense being pulverized and mixed after material mixing after pumpkin fermenting material, microbial strain culture and sieving
Spend and mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value, is granulated up to biofermentation fertilizer.
The preparation of 4 soil remediation biofermentation fertilizer of embodiment
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Beans root powder was crushed to 12 mesh sieves；Plaster, which was crushed to 12 mesh sieves and is mixed to get, is pulverized and mixed material；
S2. use mass percent dense being pulverized and mixed after material mixing after pumpkin fermenting material, microbial strain culture and sieving
Spend and mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value, is granulated up to biofermentation fertilizer.
The preparation of 5 soil remediation biofermentation fertilizer of embodiment
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Beans root powder was crushed to 12 mesh sieves；Plaster, which was crushed to 12 mesh sieves and is mixed to get, is pulverized and mixed material；
S2. use mass percent dense being pulverized and mixed after material mixing after pumpkin fermenting material, microbial strain culture and sieving
Spend and mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value, is granulated up to biofermentation fertilizer.
The preparation of 1 soil remediation biofermentation fertilizer of comparative example
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Humic acid powder was broken to 20 mesh sieves；Beans root powder was crushed to 12 mesh sieves；Plaster
It was crushed to 12 mesh sieves and was mixed to get and is pulverized and mixed material；
S2. by pumpkin fermenting material and sieving after being pulverized and mixed material mixing after use mass percent concentration for 5% sulphur
Acid for adjusting pH value mixes in proportion to 7, then with urea, dipotassium hydrogen phosphate, is granulated up to biofermentation fertilizer.
The preparation of 2 soil remediation biofermentation fertilizer of comparative example
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Beans root powder was crushed to 12 mesh sieves；S2. by being pulverized and mixed after pumpkin fermenting material, microbial strain culture and sieving
Mass percent concentration is used to be mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value after material mixing
Close, be granulated up to biofermentation fertilizer.
Comparative example 3
According to mass fraction meter, the soil remediation biofermentation fertilizer is made of in proportion following each component：
The preparation method of soil remediation biofermentation fertilizer of the present invention is as follows：
S1. by pumpkin crushed 10 mesh sieves, add equal volume water stir evenly, it is cold after 105 DEG C of high temperature sterilization 20min
But to room temperature, add 0.6 part of EM microbial bacterial agent and be stirred, seal and ferment 14 days under the conditions of 55 DEG C, wherein every 7 days turn over
Heap one time fermentation material, obtains pumpkin fermenting material；Microbial strain culture is prepared by 1 the method for embodiment；Humic acid is crushed
To 20 mesh sieves excessively；Plaster, which was crushed to 12 mesh sieves and is mixed to get, is pulverized and mixed material；
S2. use mass percent dense being pulverized and mixed after material mixing after pumpkin fermenting material, microbial strain culture and sieving
Spend and mixed in proportion to 7, then with urea, dipotassium hydrogen phosphate for 5% sulphur acid for adjusting pH value, is granulated up to biofermentation fertilizer.
Application test
1. the application effect appraisal of soil remediation biofermentation fertilizer：
Fertilizer of the present invention can provide all kinds of nutrients needed for crop growth, while effectively repair acid soil, improve soil
The ecological balance in earth.The fertilizer actual effect that large-scale demonstration is applied on crops prepared for the verification present invention, application
People's selection has carried out experiment and demonstration on Chinese cabbage.
Materials and methods
1.1 are located in Zixing City acid soil Chinese cabbage planting base for examination place 1,14 mu of testing ground area, soil fertilizer
Power level is more uniform.
1.2 for studying thing Chinese cabbage, seeding row spacing 0.4m*0.5m, the growing way golden mean of the Confucian school and consistent.
Soil remediation made from 1.3 solid fertilizers prepared for examination fertilizer for embodiment 2~4,1~3 either method of comparative example
Biofermentation fertilizer, product forms are graininess.
1.4 carry out 7 test process, 2 mu of experiment ground areas of each experiment process point for examination place：
1. 1 group of embodiment：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by embodiment 2 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
2. 2 groups of embodiment：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by embodiment 3 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
3. 3 groups of embodiment：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by embodiment 4 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
4. 4 groups of embodiment：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by embodiment 5 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
5. 1 group of comparative example：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by comparative example 1 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
6. 2 groups of comparative example：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by comparative example 2 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
7. 3 groups of comparative example：Soil is poured into water of revealing the exact details, moves into Chinese cabbage seed, by comparative example 3 prepare for examination fertilizer with
80kg/ it is per mu it is even apply, hereafter fertilising every month once, applies 5kg urea and 5kg potassium sulfates and water leaching is applied per acre.
Embodiment group and comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre to be small
Area, randomly selects 1 plant of plant measure Chinese cabbage quality status, repeats to choose 3 measure Chinese cabbage upgrowth situations, tries to achieve average value.
As a result with analysis
The influence of 2.1 pairs of Chinese cabbage yield and soil
3200 Chinese cabbages are planted per acre, and height to single Chinese cabbage, measured again, calculates per mu yield, at the same time also
Corresponding soils pH value is detected, as shown in Table 1, using single height of the Chinese cabbage of the rehabilitating soil organic fertilizer compared to pair
Ratio group adds about 20%, and single weight adds nearly half compared to comparative example group, and per mu yield also increases about 50%.
Table 1
The foregoing is merely the preferred embodiment of the present invention, is not intended to limit the invention, for those skilled in the art
For member, the invention may be variously modified and varied.Any modification within the spirit and principles of the invention, being made,
Equivalent substitution, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of soil remediation biofermentation fertilizer, it is characterised in that by dipotassium hydrogen phosphate, urea and material group containing organic matter
Into the total content of organic matter is >=44% in the biofermentation fertilizer, N P and K total content >=17.1%；The thing containing organic matter
Matter is pumpkin, humic acid, microbial strain culture, beans root powder and plaster；
The microbial strain culture is obtained by streptomyces bacterial strain " TY-001# " fermentation；The culture of streptomyces bacterial strain " TY-001# "
Base is：After taking soybean to be soaked or taking dregs of beans to carry out pre fermentation, it is beaten, is filtered；Then toward after adding sugar in filtrate, add
Water is settled to concentration >=100g/L sugared in culture medium, and pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., its deposit number is GDMCC No.：60152, preservation address is Guangzhou, Guangdong Province, China city
Five building, Institute of Micro-biology of the province laboratory building of martyr Road 100.
2. soil remediation biofermentation fertilizer according to claim 1, it is characterised in that the humic acid uses lignite weathering
Humic acid.
3. soil remediation biofermentation fertilizer according to claim 1, it is characterised in that always contain according to organic matter in fermentation fertilizer
Amount calculates, the content of organic matter >=1.2% in the beans root powder, organic matter total content >=24% in the microbial strain culture, the corruption
Organic matter total content >=3% in phytic acid.
4. soil remediation biofermentation fertilizer according to claim 1, it is characterised in that according to mass fraction meter, by following
Each component forms in proportion：
10 ~ 16 parts of dipotassium hydrogen phosphate；
12 ~ 18 parts of urea；
10 ~ 16 parts of pumpkin；
8 ~ 16 parts of humic acid；
30 ~ 40 parts of microbial strain culture；
8 ~ 14 parts of beans root powder；
8 ~ 14 parts of plaster.
5. soil remediation biofermentation fertilizer according to claim 1, it is characterised in that according to mass fraction meter, by following
Each component forms in proportion：
14 parts of dipotassium hydrogen phosphate；
15 parts of urea；
14 parts of pumpkin；
12 parts of humic acid；
36 parts of microbial strain culture；
12 parts of beans root powder；
12 parts of plaster.
6. soil remediation biofermentation fertilizer according to claim 1, it is characterised in that the beans root powder is plucked by legume
Remaining is gone after fruit, stem, leaf crush after drying and obtain.
7. a kind of preparation method of soil remediation biofermentation fertilizer, it is characterised in that comprise the following steps：
S1. pumpkin is crushed plus water, high temperature sterilization postcooling, addition bio-fermentation agent is fermented to obtain pumpkin fermenting material；It is accurate
Standby microbial strain culture；Humic acid, beans root powder, plaster are pulverized and sieved, is mixed to get and is pulverized and mixed material；
S2. pH value is adjusted by pumpkin fermenting material, microbial strain culture and after being pulverized and mixed material mixing, then is pressed with urea, dipotassium hydrogen phosphate
Ratio mixes, and is granulated up to biofermentation fertilizer.
8. soil remediation biofermentation fertilizer according to claim 7, it is characterised in that the bio-fermentation agent is lactic acid
Bacterium, saccharomycete, photosynthetic bacteria, bacillus category, the one or more in filamentous fungi, lactobacillus plantarum, EM microbial bacterial agents.
9. soil remediation biofermentation according to claim 7 fertilizer, it is characterised in that the humic acid powder was broken to 20 ~
30 mesh sieves；Beans root powder was crushed to 10 ~ 20 mesh sieves；Plaster was crushed to 10 ~ 20 mesh sieves.
10. soil remediation biofermentation fertilizer according to claim 7, it is characterised in that the bio-fermentation agent and pumpkin
The ratio between number is 1:15~20.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108456041A (en) * | 2018-06-06 | 2018-08-28 | 佛山科学技术学院 | A kind of reparation fermentation fertilizer rich in trace element |
| CN111543230A (en) * | 2020-03-30 | 2020-08-18 | 同济大学 | Large-scale blueberry planting method suitable for mountainous regions |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103274866A (en) * | 2013-06-17 | 2013-09-04 | 山东亿丰源生物科技股份有限公司 | Organic inorganic compound fertilizer containing biological germs and preparation method thereof |
| CN107235808A (en) * | 2017-08-02 | 2017-10-10 | 合肥浦邦农业科技有限公司 | A kind of organic fertilizer and preparation method thereof |
| CN107353127A (en) * | 2017-08-23 | 2017-11-17 | 佛山推启农业研究院（普通合伙） | A kind of microorganism Water soluble fertilizer for preventing and treating corn fungal disease |
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2017
- 2017-12-29 CN CN201711497820.6A patent/CN107935788A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103274866A (en) * | 2013-06-17 | 2013-09-04 | 山东亿丰源生物科技股份有限公司 | Organic inorganic compound fertilizer containing biological germs and preparation method thereof |
| CN107235808A (en) * | 2017-08-02 | 2017-10-10 | 合肥浦邦农业科技有限公司 | A kind of organic fertilizer and preparation method thereof |
| CN107353127A (en) * | 2017-08-23 | 2017-11-17 | 佛山推启农业研究院（普通合伙） | A kind of microorganism Water soluble fertilizer for preventing and treating corn fungal disease |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108456041A (en) * | 2018-06-06 | 2018-08-28 | 佛山科学技术学院 | A kind of reparation fermentation fertilizer rich in trace element |
| CN111543230A (en) * | 2020-03-30 | 2020-08-18 | 同济大学 | Large-scale blueberry planting method suitable for mountainous regions |
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