CN108059575A - A kind of bio-feritlizer and preparation method - Google Patents
A kind of bio-feritlizer and preparation method Download PDFInfo
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- CN108059575A CN108059575A CN201810082553.4A CN201810082553A CN108059575A CN 108059575 A CN108059575 A CN 108059575A CN 201810082553 A CN201810082553 A CN 201810082553A CN 108059575 A CN108059575 A CN 108059575A
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- bacterium
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- culture substrate
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to fertilizer preparing technical fields, disclose a kind of bio-feritlizer and preparation method, and the component of the bio-feritlizer is made of culture substrate and carrier matrix；Culture substrate is by glucose, yeast extract, beef extract, peptone, one grade fermemtation bacterium, second order fermentation bacterium, three grade fermemtation bacterium；Carrier matrix is made of soya-bean cake, rapeseed cake, wheat bran；Mass ratio is 1: 2: 3；A kind of preparation method is disclosed simultaneously.Present invention employs syntaxial system can be formed with most crops, promote the arbuscular mycorrhizal fungi of plant growth；Why rhizobium fertilizer can generate stable effect, be that it can form syntaxial system with plant, arbuscular mycorrhizal fungi is the another kind of microorganism that syntaxial system can be formed with various plants, it can promote plant growth, improve crop yield, have stable effect.
Description
Technical field
The invention belongs to fertilizer preparing technical field more particularly to disclose a kind of bio-feritlizer and preparation method.
Background technology
Nearly 1.4 hundred million hectares of China's cultivated area, national population about 1,300,000,000,0.1 hectare of per capita area of cultivated farmland are only people from world
The 37.5% of equal 0.27 hectare of cultivated area.On existing soil, to meet the needs of people are growing to agricultural product, carry
High multiple crop index event so makes total output improve.There is certain difficulty in large arable land again, and practical ways are exactly to carry
High yield per unit area.
Fertilizer is that alternatively fertilizer is basis for the survival of mankind to a certain extent for the grain of crop.Chronic administration
Chemical fertilizer pollutes underground water, soil hardening, and reduces soil fertility and pollute the environment, air it is muddy and
To people's body is damaged, disease increases other various factors.
From nineteen ninety China propose pollution-free food concept advocate production pollution-free food through into complete set production and
Management system is a complete quality control system engineering, by agronomy, ecology, environmental science, nutrition, hygiene etc.
Production, processing, transport, the sale that the principle of subject applies to food are complete nuisanceless, free of contamination so as to form one
High-quality production-supply-marketing system is from the whole-course quality control in " soil to dining table ", and its main purpose is to safeguard ecological environment and control
Harmful content in agricultural products processed.
In conclusion problem existing in the prior art is：
Existing bio-feritlizer does not utilize the compost powder of strong adsorption force full of nutrition to make culture base adsorbent；Do not use energy
Syntaxial system is formed with most crops, promotes the arbuscular mycorrhizal fungi of plant growth；Do not utilize microbionation agent and clump branch bacterium
Mycorrhiza fungi Inoculant interaction, reaching has good synergy；It does not select, develop some new strains, further carry
Its high validity and accommodation；And existing bio-feritlizer is environmentally friendly poor.
The content of the invention
In view of the problems of the existing technology, the present invention provides a kind of bio-feritlizer and preparation methods.
The present invention is achieved in that a kind of bio-feritlizer, and the component of the bio-feritlizer is by culture substrate and carrier base
Matter forms；
The culture substrate is by glucose, yeast extract, beef extract, peptone, one grade fermemtation bacterium, second order fermentation bacterium, three-level
Zymophyte；The one grade fermemtation bacterium, second order fermentation bacterium, living bacteria count >=0.50 hundred million/g of three grade fermemtation bacterium；
The carrier matrix is made of soya-bean cake, rapeseed cake, wheat bran；Mass ratio is 1: 2: 3.
The soya-bean cake, rapeseed cake, wheat bran are crushed as 60 mesh and sieved.
Culture substrate and carrier matrix are respectively by mass parts：1 part and 15 parts；The glucose, yeast extract, beef extract,
Peptone is 1: 1.5: 5: 2 in mass ratio.
Another object of the present invention is to provide a kind of preparation method of bio-feritlizer to include：
Culture substrate is prepared, level-one, two level, three grade fermemtation bacterium add in glucose, beef extract, peptone；It goes out by high pressure
Bacterium pot 121 DEG C of autoclave sterilizations be cooled to 28 DEG C it is spare；
The preparation of carrier matrix, soya-bean cake, rapeseed cake, wheat bran are screened, and are crushed, after crossing the sieve of 60 mesh sizes, according to 1: 2: 3
: 1 ratio enters in high-temp solid reaction agitator tank, adds in the trace element needed for plant and is mixed evenly, and stirring is equal
Even material into 121 celsius temperature high pressure sterilizations 2 it is small when；
Recirculated water in high-temp solid reaction agitator tank internal layer is standby by the 28 of the culture substrate cooling to have sterilized row degree Celsius
With；
Liquid bacterium solution is added in into solid reflection stirring by cultured liquid bacterium solution by measuring tank according to 25% ratio
It is stirred in tank 30 minutes；
Add in trace element stir evenly rear accumulated solids fermentation 48 it is small when；The solid biofertilizer fermented and mycorhiza are true
Bacterium separately mix be sent into conveyer belt into metering packing case by defined measuring requirement metering packing into packing material.
Further, after being packaged into packing material, detection warehousing after passing, for sale.
Further, the cultural method of liquid bacterium solution includes：Former 28 DEG C of cultures of strain inclined plane, isoclinic face covers with lawn, by bacterium
Tongue carries out 50 liters of shaking flask activation culture, is connected in 100 liters of the culture substrate level liquid culture medium of preparation and carries out level-one expansion training
Support 24 it is small when；Level-one expansion cultivated bacterium number reach after 800,000,000 transferring in preparation culture substrate two level expand culture medium
Continue in 1000 liters expand culture 24 it is small when；Cultured bacterium solution automatic transfer expands culture medium in the culture substrate three-level of preparation
It is cultivated when progress 24 is small in 5000 liters；It is spare to cultivate successful bacterium solution storage.
Advantages of the present invention and good effect are：
Make culture base adsorbent with the compost powder of strong adsorption force full of nutrition.
Nutrition is the essential factor of microbial growth, and bio-feritlizer routine production method is will to have bred
Bacterial adsorption on lignite, lignite have preferable absorption property but lack nutrition.The important innovations point of composite bio-fertilizer it
When will by fermentation reactor system, all kinds of grouts fermentates based on dregs of beans, and add suitable nitrogen, phosphorus, potassium, calcium, zinc,
The elements such as manganese, iron, magnesium are used as adsorbent, and since they are full of nutrition, microorganism can continue to breed and tie up in this environment
Its survival is held, thus its living bacteria count is still kept in the longer term, meanwhile, this adsorbent full of nutrition is applied with strain
After entering soil, contribute to field planting and transition of the strain in new environment, particularly in poor soil, can more play this work
With.
Syntaxial system can be formed with most crops by employing, and promote the arbuscular mycorrhizal fungi of plant growth.
Why rhizobium fertilizer can generate stable effect, be that it can form syntaxial system, arbuscular mycorrhiza with plant
Fungi is the another kind of microorganism that syntaxial system can be formed with various plants, it can promote plant growth, improve crop yield,
With stable effect.The present invention, which has been found out, a set of to be produced arbuscular mycorrhizal fungi Inoculant by host plant and has efficacious prescriptions
Method can be produced on a large scale.Therefore, composite bio-fertilizer of the invention, which adds this, can bring the true of stablizing effect
Bacterium Inoculant.
Microbionation agent has good synergy with arbuscular mycorrhizal fungi Inoculant interaction.
Rhizobium and the double inoculations of mycorrhizal fungi are carried out when planting legume can bring higher effect of increasing production wide
To confirm, experimental study in recent years also demonstrate some azotobacterias, growth-promoting bacterium can be generated with mycorrhizal fungi interaction it is good
Synergy.2000 and 2,001 two the present invention Suizhou City Zengdu District paddy drought seedling and transplanted with mud to crop field, cotton
The field trial of the crops such as flower feeding block seedlings raising, corn, peanut, cowpea, tobacco confirms this result again.Singly apply bacterium
Fertilizer singly applies the processing of mycorrhizal fungi and Matrix controls ratio, and effect of increasing production reaches the level of signifiance, and carries out the processing of double inoculations, then reaches
Extremely significant effect of increasing production is arrived.Therefore, the technology path that the present invention uses is production microbionation agent and mycorrhizal fungi inoculation
The NEW TYPE OF COMPOSITE life fertilizer of agent joint compounding.This also has found an improvement way singly to apply the defects of bacterial fertilizer effect is unstable
Footpath, this measure are most prominent and most important innovations of the invention.
The present invention selects, develops some new strains.
Some has been selected to have the rhizobium of the different cross inoculation groups of growth-promoting functions, the stronger bacterial strain of nitrogen fixing capacity and infectivity
Can well arbuscular mycorrhizal fungi of high fertility etc. be resistant to again, after these strains are added to this composite bacterial fertilizer, can further improved
Its validity and accommodation.
Product of the present invention is good for the environment.
Mycorrhizal fungi mycelia in composite bio-fertilizer can absorb heavy metal ion, can reduce heavy metal in soil ion pair
The pollution of crops；Product of the present invention contains the photosynthetic bacteria strong to multiple pollutant degradation capability.The fertilizer of the present invention is one
It is a to be again good for the environment to plant offer abundant nutrition, it can be as the means of production of pollution-free food production recommendation.This hair
The compost powder adsorbent of replacement lignite used in bright product, is also Agricultural Integration Leading Enterprises --- large-scale poultry place, pig raising
The excrement of field, harmless treatment, elimination pollution, promotion value have found effective approach.
Bio-feritlizer of the present invention, nutritional ingredient is balanced, and rich in various trace elements, it can by the microorganism species of addition
Nutrient is persistently provided to absorb for vegetation period.And there are the microbial bacterial agent in the present invention other harmful bacterias of inhibition to carry
High soil fertility effect.And effectively improve the micro-ecological environment of soil.
Description of the drawings
Fig. 1 is the preparation method flow chart of bio-feritlizer provided in an embodiment of the present invention.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described the application principle of the present invention.
Bio-feritlizer provided in an embodiment of the present invention, the component of the bio-feritlizer is by culture substrate and carrier matrix group
Into；
The culture substrate is by glucose, yeast extract, beef extract, peptone, one grade fermemtation bacterium, second order fermentation bacterium, three-level
Zymophyte；The one grade fermemtation bacterium, second order fermentation bacterium, living bacteria count >=0.50 hundred million/g of three grade fermemtation bacterium；
The carrier matrix is made of soya-bean cake, rapeseed cake, wheat bran；Mass ratio is 1: 2: 3.
The soya-bean cake, rapeseed cake, wheat bran are crushed as 60 mesh and sieved.
Culture substrate and carrier matrix are respectively by mass parts：1 part and 15 parts；The glucose, yeast extract, beef extract,
Peptone is 1: 1.5: 5: 2 in mass ratio.
Shown in Fig. 1, the preparation method of bio-feritlizer provided in an embodiment of the present invention, including：
According to bacterial strain need prepare culture substrate.Main raw material(s) used in culture medium：Glucose, yeast extract, beef
Cream, peptone；Level-one, two level, three grade fermemtation add in glucose, beef extract, peptone；By 121 DEG C of height of high-pressure sterilizing pot
Warm high pressure sterilization be cooled to 28 DEG C it is spare.
The cultural method of liquid bacterium solution includes：Former 28 DEG C of cultures of strain inclined plane, isoclinic face cover with lawn, lawn are shaken
Bottle 50 liters of activation culture, be connected in 100 liters of the culture substrate level liquid culture medium of preparation carry out level-one expand culture 24 it is small when；
Level-one expansion has been cultivated bacterium number and reached expands 1000 liters of culture medium relaying after 800,000,000 in the culture substrate two level in preparation of transferring
It is continuous expand culture 24 it is small when；Cultured bacterium solution automatic transfer in preparation culture substrate three-level expand 5000 liters of culture medium in into
Row 24 is cultivated when small；It is spare to cultivate successful bacterium solution storage.
The preparation of carrier matrix.The main raw material(s) of matrix is soya-bean cake, rapeseed cake, wheat bran carry out screening operation, and crushing reaches
It can enter according to 1: 2: 3 ratio in high-temp solid reflection agitator tank, add in micro- needed for plant after sieve by 60 mesh sizes
Secondary element is mixed evenly, the material stirred evenly into 121 celsius temperature high pressure sterilizations 2 it is small when.Cycling in internal layer
Water is spare by 28 degrees Celsius of the culture substrate cooling to have sterilized.By cultured liquid bacterium solution by measuring tank according to 25%
Liquid bacterium solution is added in solid reaction agitator tank and is stirred 30 minutes by ratio.It adds in appropriate trace element and stirs evenly rear heap
When product solid fermentation 48 is small.The solid biofertilizer fermented separately mixes with mycorrhizal fungi and is sent into conveyer belt to metering packing case
In by defined measuring requirement metering packing into packing material.It is combined as finished product.Detect warehousing after passing, for sale.
Strain of the present invention is purchased from market.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of preparation method of bio-feritlizer, which is characterized in that the preparation method of the bio-feritlizer includes：
Culture substrate is prepared, level-one, two level, three grade fermemtation bacterium add in glucose, beef extract, peptone；By high-pressure sterilizing pot
121 DEG C of autoclave sterilizations be cooled to 28 DEG C it is spare；
The preparation of carrier matrix, soya-bean cake, rapeseed cake, wheat bran are screened, and are crushed, after crossing the sieve of 60 mesh sizes, according to 1: 2: 3: 1
Ratio is poured into high-temp solid reaction agitator tank, is added in the trace element needed for plant and is mixed evenly, what is stirred evenly
Material into 121 celsius temperature high pressure sterilizations 2 it is small when；
Recirculated water in high-temp solid reaction agitator tank internal layer is spare by 28 degrees Celsius of the culture substrate cooling to have sterilized；
Cultured liquid bacterium solution is added in liquid bacterium solution in solid reaction agitator tank according to 25% ratio by measuring tank
It is stirred 30 minutes；
Add in trace element stir evenly rear accumulated solids fermentation 48 it is small when；The solid biofertilizer fermented and mycorrhizal fungi are another
Row mixing is sent into conveyer belt and defined measuring requirement metering packing is pressed into metering packing case into packing material.
2. the preparation method of bio-feritlizer as described in claim 1, which is characterized in that after being packaged into packing material, detection is qualified
It is put in storage afterwards, is for sale.
3. the preparation method of bio-feritlizer as described in claim 1, which is characterized in that the cultural method of liquid bacterium solution includes：It is former
28 DEG C of cultures of strain inclined plane, isoclinic face cover with lawn, and lawn is carried out 50 liters of shaking flask activation culture, is connected to the culture substrate of preparation
When progress level-one expansion culture 24 is small in 100 liters of level liquid culture medium；Level-one expansion has been cultivated to be carried out after bacterium number reaches 800,000,000
Transfer in preparation culture substrate two level expand 1000 liters of culture medium in continue expand culture 24 it is small when；Cultured bacterium solution is automatic
It transfers when progress 24 is small in culture substrate three-level 5000 liters of the culture medium of expansion of preparation and cultivates；It is standby to cultivate successful bacterium solution storage
With.
4. bio-feritlizer prepared by a kind of preparation method of bio-feritlizer as described in claim 1, which is characterized in that the biology
The component of fertilizer is made of culture substrate and carrier matrix；
The culture substrate is by glucose, yeast extract, beef extract, peptone, one grade fermemtation bacterium, second order fermentation bacterium, three grade fermemtation
Bacterium；The one grade fermemtation bacterium, second order fermentation bacterium, living bacteria count >=0.50 hundred million/g of three grade fermemtation bacterium；
The carrier matrix is made of soya-bean cake, rapeseed cake, wheat bran；Mass ratio is 1: 2: 3.
5. bio-feritlizer as claimed in claim 4, which is characterized in that the soya-bean cake, rapeseed cake, wheat bran are crushed as 60 mesh and mistake
Sieve.
6. bio-feritlizer as claimed in claim 4, which is characterized in that culture substrate and carrier matrix are respectively by mass parts：1 part
With 15 parts；The glucose, yeast extract, beef extract, peptone are 1: 1.5: 5: 2 in mass ratio.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810082553.4A CN108059575A (en) | 2018-01-23 | 2018-01-23 | A kind of bio-feritlizer and preparation method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150324A (en) * | 2019-07-03 | 2019-08-23 | 安徽新熙盟生物科技有限公司 | A kind of compound nitragin, preparation method and application |
| CN110407648A (en) * | 2019-08-12 | 2019-11-05 | 上海植物园 | A kind of acidic microbial organic fertilizer and its preparation method and application |
| CN110437011A (en) * | 2019-08-12 | 2019-11-12 | 上海植物园 | A kind of azalea special bio fertilizer and its production method |
| CN110437001A (en) * | 2019-08-16 | 2019-11-12 | 中国科学院生态环境研究中心 | Radix Glycyrrhizae special bio organic composite fertilizer and application |
| CN115504839A (en) * | 2022-09-27 | 2022-12-23 | 苏州农业职业技术学院 | Microbial compound bacterial fertilizer and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101659570A (en) * | 2008-08-28 | 2010-03-03 | 湖北正佳微生物工程股份有限公司 | Preparation method of biofertilizer |
-
2018
- 2018-01-23 CN CN201810082553.4A patent/CN108059575A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101659570A (en) * | 2008-08-28 | 2010-03-03 | 湖北正佳微生物工程股份有限公司 | Preparation method of biofertilizer |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150324A (en) * | 2019-07-03 | 2019-08-23 | 安徽新熙盟生物科技有限公司 | A kind of compound nitragin, preparation method and application |
| CN110407648A (en) * | 2019-08-12 | 2019-11-05 | 上海植物园 | A kind of acidic microbial organic fertilizer and its preparation method and application |
| CN110437011A (en) * | 2019-08-12 | 2019-11-12 | 上海植物园 | A kind of azalea special bio fertilizer and its production method |
| CN110437001A (en) * | 2019-08-16 | 2019-11-12 | 中国科学院生态环境研究中心 | Radix Glycyrrhizae special bio organic composite fertilizer and application |
| CN115504839A (en) * | 2022-09-27 | 2022-12-23 | 苏州农业职业技术学院 | Microbial compound bacterial fertilizer and preparation method thereof |
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