CN110150324A - A kind of compound nitragin, preparation method and application - Google Patents
A kind of compound nitragin, preparation method and application Download PDFInfo
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- CN110150324A CN110150324A CN201910594838.0A CN201910594838A CN110150324A CN 110150324 A CN110150324 A CN 110150324A CN 201910594838 A CN201910594838 A CN 201910594838A CN 110150324 A CN110150324 A CN 110150324A
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- nitragin
- compound
- vesicular
- spore
- rhizobium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
The present invention provides a kind of compound nitragins comprising rhizobium, vesicular-arbuscular mycorrhiza bacterium and thick spore Verticillium dahliae.Wherein, rhizobium have the function of fixed nitrogen getting fat；Vesicular-arbuscular mycorrhiza bacterium has the function of promoting crop root growth and enhances nutrient absorption, it is used in combination with rhizobium can promote the breeding of rhizobium to increase and strengthen the nitrogen fixation effect of rhizobium；Thick spore Verticillium dahliae can prevent and treat root nematode disease, effectively play root guard effect.The compound nitragin has fixed nitrogen getting fat, root guard of taking root, and prevents and treats root insect pest, promotes plant growth, realizes the effect that crops are substantially increased production.
Description
Technical field
The present invention relates to field of agricultural microbial technology, and in particular to a kind of compound nitragin and is answered at preparation method
With.
Background technique
Containing there are about 80% nitrogen in air, but general crops can not directly utilize, and most of crops are only logical
The symbiotic azotification with rhizobium is crossed, the utilizable ammoniacal nitrogen of crop the molecular nitrogen in air can be just converted into.
After germination takes root, rhizobium invade root from root hair, form the root nodule with nitrogen fixing capacity under certain condition.Solid
Under the action of nitrogen enzyme, molecular nitrogen is converted ammoniacal nitrogen by the bacteroid in root nodule.At the same time, each root nodule is exactly one micro-
Nitrogen is continuously conveyed to plant and utilized by type nitrogen fertilizer plant.Environment is not had by the nitrogenous fertilizer that rhizobium generate crops
Pollution does not have nitrogen discharge without long-distance transport, in use process, and manually apply the turnover rate of chemical nitrogen fertilizer commonly greater than
50%.
However, the Indigenous Rhizobia quantity around root system is seldom, it is difficult to meet plant growth for non-fixed nitrogen class crop
Needs, require supplementation with nitragin.It is found in production practices, single supplement nitragin effect is not significant, and reason is mainly
Crop root especially root gross density is inadequate, and the object of rhizobium intrusion is less or crop root is encroached on by nematode in soil, leads
Too poor etc. reasons of root condition are caused to cause.Therefore it needs to provide a kind of microbial inoculum containing rhizobium, has and promote crop root
The effects of growth, enhancing nitrogen fixation effect.
In consideration of it, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of compound nitragin.
The second object of the present invention is to provide the preparation method of the compound nitragin.
The third object of the present invention is to provide the application of compound nitragin in agricultural production.
To achieve the above object, technical scheme is as follows:
The present invention relates to a kind of compound nitragins, including rhizobium, vesicular-arbuscular mycorrhiza bacterium and thick spore Verticillium dahliae.
Preferably, the compound nitragin includes nitragin, vesicular-arbuscular mycorrhiza spore microbial inoculum and thick spore Verticillium dahliae
Agent.
Preferably, the compound nitragin includes following components according to parts by weight:
Preferably, the compound nitragin is made of following components according to parts by weight:
Preferably, in the compound nitragin, effective bacterium number of rhizobium is hundred million/g of 10-20, vesicular-arbuscular mycorrhiza bacterium
Effective bacterium number be hundred million/g of 1-2, effective bacterium number of thick spore wheel branch bacillus is hundred million/g of 2-5.
Preferably, the vesicular-arbuscular mycorrhiza spore microbial inoculum is prepared by high cell density fermentation.
Preferably, contain following component in every 1 high-density fermentation liquid: fructose 6g, natural plant gum 8g, glycine 0.2g, dried meat
Propylhomoserin 0.05g, maltose 10g, magnesium sulfate 0.6g, potassium chloride 0.08g, inositol 0.05g, ammonium molybdate 0.0002g, niacin
0.0006g, copper sulphate 0.0002g, boric acid 0.005g, remainder are pure water.
Preferably, the preparation process the following steps are included:
The pH value of the high density fermentation liquid is adjusted to 6.5 or so, 121 DEG C of sterilizings after twenty minutes, 25 DEG C is cooled to, passes through
Sterile working accesses Vesicular arbuscular mycorriza spore, and then at 24~26 DEG C, revolving speed is 50~80rpm/min, ventilating ratio 1:
0.3 (V/Vm), tank pressure are cultivated 15 days under the conditions of being 0.03~0.05MPa, obtain the vesicular-arbuscular mycorrhiza bacterium spore microbial inoculum.
The invention further relates to the preparation methods of the compound nitragin, comprising the following steps: the conical double ribbon of Xiang Yunhang
It revolves and diatomite and mannitol is added in mixing machine, then sequentially add nitragin, thick spore Verticillium dahliae agent and vesicular-arbuscular mycorrhiza
Spore microbial inoculum stops stirring after mixing, is then packed and stored.
Preferably, the mixing temperature of conical twin-screw mixer is 15~25 DEG C.
Preferably, incorporation time is 15~30 minutes.
The invention further relates to the application of the compound nitragin in agricultural production.
Beneficial effects of the present invention:
The present invention provides a kind of compound nitragins comprising rhizobium, vesicular-arbuscular mycorrhiza bacterium and thick spore Verticillium dahliae.
Wherein, rhizobium have the function of fixed nitrogen getting fat；Vesicular-arbuscular mycorrhiza bacterium, which has, promotes crop root growth and enhancing nutrition to inhale
The effect of receipts, it is used in combination with rhizobium can promote the breeding of rhizobium to increase and strengthen the nitrogen fixation effect of rhizobium；
Thick spore Verticillium dahliae can prevent and treat root nematode disease, effectively play root guard effect.The compound nitragin has fixed nitrogen getting fat, shield of taking root
Root prevents and treats root insect pest, promotes plant growth, realizes the effect that crops are substantially increased production.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
The present embodiments relate to a kind of compound nitragins, including rhizobium, vesicular-arbuscular mycorrhiza bacterium and thick spore wheel branch
Bacterium.
Wherein, rhizobium (Rhizobium) refer mainly to the bacterium that root nodule and energy fixed nitrogen are formed with the symbiosis of legume crop root,
Refer generally to rhizobium and Bradyrhizobium；Two categories belong to Rhizobiales order.Rhizobia cell is in the shape of a rod, amphitrichous and pod
Film, flagellum are normal movable organ, no gemma, Gram-negative, category aerobic-type.The thallus form lived in root nodule
It is varied, there are the types such as pyriform, clavate type or T, X, Y, the thallus of this deformation becomes bacteroid；Bacteroid is in leguminous plant body
Inside not growth and breeding, but can have good action to leguminous plant with the common fixed nitrogen of leguminous plant.Rhizobium invade host Gen Nei,
The certain cells for stimulating root cortex and pericycle, cause the intensive growth of these cells, and the local bulkiness of root is made to form root nodule；
Rhizobium settle down in root, and plant supplies rhizobium with mineral nutriment and the energy, and rhizobium fix the nitrogen that dissociates in atmosphere, to plant
Object provides nitrogen nutriment, and the two is at equilibrium in antagonism parasitism and shows symbiosis.
Vesicle-arbuscular mycorrhiza is also known as arbuscular mycorrhiza (Vesicalar-Arbuscular), i.e. VA mycorhiza, is Endogone
(Endogonaceae) syntaxial system that some fungi and plant roots is formed.The outer mycelia of a small number of roots of vesicular-arbuscular mycorrhiza has thickness
Two kinds of tiny branch mycelia of the thick mycelia of wall and thin-walled, can also form thin-walled folliculus, chlamydospore, engagement on thick mycelia
Spore etc..Relationship with plant includes following several respects: 1. photosynthesis of plant provides carbon source and energy for the growth and development of fungi
Source.2. arbuscular mycorrhiza increases the range of root circle, absorption of the root system to moisture is increased, improves the drought-resistant ability of plant, is improved
Plant nutrient condition.3. arbuscular mycorrhiza can expand crop root absorption region, the suction that nutriment is absorbed from the soil liquid is improved
Yield.4. promoting the nitrogen-fixing bacteria of rhizosphere microorganism, phosphobacteria growth, and there is good shadow to the dross of symbiotic nitrogen fixation microorganism
It rings.
Thick wall verticillium sp (thick spore Verticillium dahliae) is a kind of facultative parasite, can rely on one's own efforts in the soil and seek saprophytic life
It is living, and the stronger plant line of the anchorages such as spherical cyst nematode, cyst nematode and root-knot nematode that can parasitize plant rhizosphere area
Worm.Although thick spore Verticillium dahliae can also infect the epiblem of plant, it will not further infect the dimension tube layer inside root, so not
It can cause a disease in plant.Since thick spore Verticillium dahliae is pure biological agent, phytotoxicity is basically will not produce to crop, peace is compared to people and animals
Entirely.The noresidue in environment, crop, no pollution to the environment after application.
Further, compound nitragin includes nitragin, vesicular-arbuscular mycorrhiza spore microbial inoculum and thick spore Verticillium dahliae agent.
Wherein, nitragin and thick spore Verticillium dahliae agent can directly be bought.Nitragin refers to rhizobium for production strain
Manufactured microorganism formulation product, it is capable of fixing the nitrogen in air, a large amount of nitrogenous fertilizer is provided for host plant, to reach
The purpose of volume increase, the Rhizobium Inoculation when not planting green manure or new clearage plantation leguminous green manure for many years, can ensure that leguminous green manure is raw
It is long good.Nitragin can be prepared by microbial fermentation processes.
The effective component of thick spore Verticillium dahliae agent is thick spore Verticillium dahliae spore, is made of microorganism fermentation process, at present state
Interior existing Luliang Mei Deng company production.
Currently, the country there is no commercially available vesicular-arbuscular mycorrhiza spore microbial inoculum.The vesicular-arbuscular mycorrhiza spore that the present invention uses
Microbial inoculum is prepared by the following method to obtain:
(1) strain source and cultural method:
Anhui Suzhou City Bian He rhizoma imperatae nearby is acquired, screening is isolated and obtains arbuscular mycorrhizal fungi spore as fermentation
Bacterial strain produces microbial inoculum by high cell density fermentation.
(2) high density fermentation liquid ingredient:
Contain following component in every 1 high-density fermentation liquid: fructose 6g, natural plant gum 8g, glycine 0.2g, proline
0.05g, maltose 10g, magnesium sulfate 0.6g, potassium chloride 0.08g, inositol 0.05g, ammonium molybdate 0.0002g, niacin 0.0006g, sulphur
Sour copper 0.0002g, boric acid 0.005g, remainder are pure water.
(3) incubation step:
The pH value of above-specified high density fermentation liquid is adjusted to 6.5 or so, 121 DEG C of sterilizings after twenty minutes, 25 DEG C is cooled to, passes through
Sterile working accesses Vesicular arbuscular mycorriza spore, and is cultivated in stainless steel fermentation tank.At 24~26 DEG C, revolving speed is
50~80rpm/min, ventilating ratio 1:0.3 (V/Vm), tank pressure are cultivated 15 days under the conditions of being 0.03~0.05MPa.
Diatomite is added by the 8% of fermentating liquid volume, high speed centrifugation is carried out after being uniformly mixed, removes supernatant and obtains
Concentrated phase is dried to get vesicular-arbuscular mycorrhiza bacterium spore microbial inoculum is arrived with vacuum drier.
In one embodiment of the invention, which includes following components according to parts by weight:
Wherein, mannitol is the isomer of D-sorbite, soluble easily in water, is the solid of white clear, there is similar sugarcane
The sweet taste of sugar.It can be obtained by being extracted from kelp, act on and provide nutritious carbon sourc for rhizobial growth.Diatomite is a kind of siliceous
Rock is mainly made of the remains of ancient times diatom.Its chemical component is with SiO2Based on.It, can be effective with porous structure
The microorganism in microbial inoculum is adsorbed, prevents from being lost.
Further, which is made of following components according to parts by weight:
In one embodiment of the invention, effective bacterium number of rhizobium is hundred million/g of 10-20, bubble in the compound nitragin
Effective bacterium number of capsule arbuscular mycorrhizal fungus is hundred million/g of 1-2, and effective bacterium number of thick spore wheel branch bacillus is hundred million/g of 2-5.
The invention further relates to the preparation methods of the compound nitragin, comprising the following steps: the conical double ribbon of Xiang Yunhang revolves
The diatomite and mannitol of above-mentioned usage ratio are added in mixing machine, then sequentially add nitragin, thick spore Verticillium dahliae agent and
Vesicular-arbuscular mycorrhiza spore microbial inoculum stops stirring after mixing, is then packed and stored.
In one embodiment of the invention, the mixing temperature of conical twin-screw mixer is 15~25 DEG C.Temperature is excessively high
It will lead to strain inactivation, temperature is too low, is difficult to be uniformly mixed.It is stirred under the low or first gear of conical twin-screw mixer mixed
It closes.
In one embodiment of the invention, since diatomite and mannitol do not have bioactivity, in whipping process not
Easy in inactivation, in addition both substances can play the role of filler simultaneously, therefore first be added in whipping process.All substances are all
After addition, incorporation time is that can discharge for 15~30 minutes.
The invention further relates to the application of compound nitragin in agricultural production.As soybean, wheat, tobacco leaf, vegetable
The base manure use of dish, Rice Cropping, can make crop yield 10%~40%, save fertilizer 20%~60%.
Preparation example
According to the formula in following operating procedure and table 1, compound nitragin 1~5 is prepared.
Wherein, thick spore Verticillium dahliae agent is purchased from: the micro- source of students Biotechnology Co., Ltd in Yunnan, English name are
Verticiliumchlamydosporiumzk7；Nitragin is purchased from: Leading Bio-agricultural Co., Ltd. (former Qinhuangdao
Leading development in science and technology Co., Ltd)；Vesicular-arbuscular mycorrhiza spore microbial inoculum is prepared by the method recorded in specific embodiment
It arrives.
Table 1
The preparation process of above-mentioned compound nitragin the following steps are included:
(1) nitragin, vesicular-arbuscular mycorrhiza spore microbial inoculum, thick spore Verticillium dahliae are successively weighed by the component ratio in table 1
Agent, mannitol and diatomite；
(2) start conical twin-screw mixer, be adjusted to low or first gear operation, open cooling water, control mixing machine temperature 20
DEG C or so；
(3) diatomite and mannitol are first added into double worm mixer, then sequentially adds nitragin, thick spore wheel branch
Microbial inoculum and vesicular-arbuscular mycorrhiza spore microbial inoculum, mixed on low speed 15 minutes；
(4) stop stirring, put mixture and enter packing machine feed bin.Packed with aluminium foil bag, specification be 500g/ bags, every 10
One case of bag, is packed and stored.
Embodiment
(1) soybean in field is tested
Test carries out in Zhenan County Hui Gu, Xiuzhou City, Anhui Province Yongqiao District agricultural university experimental plot.Soil fertility is medium, cultivation condition,
Water and fertilizer management is consistent.In soybean seedling, the compound nitragin that 5kg preparation example 1 obtains is applied in root cave per acre, is covered after applying with soil
Lid prevents sunlight from irradiating and keeping microbial inoculum humidity.Control group carries out conventional fertilizer application, does not apply bacteria agent.According to following formula
It calculates rate of growth and saves fertilizer rate:
Rate of growth=(compound nitragin administration group yield-control group yield)/control group yield × 100%
Save fertilizer rate=(compound nitragin administration group rate of fertilizer-control group rate of fertilizer)/control group fertilizer
Expect amount of application × 100%
It is calculated, which is 22.5% to the average rate of growth of soybean, and saving fertilizer rate is 35.0%.
(2) wheat field trial
When spring wheat is turned green, the compound nitragin that 8kg preparation example 2 obtains is applied in root cave per acre, is covered after applying with soil
Lid prevents sunlight from irradiating and keeping microbial inoculum humidity.Control group carries out conventional fertilizer application, does not apply bacteria agent.
It is calculated, which is 17.2% to the average rate of growth of wheat, and saving fertilizer rate is 31.7%.
(3) tobacco leaf field trial
In tobacco leaf Seedling Stage, the compound nitragin that 8kg preparation example 3 obtains is applied in root cave per acre, with soil covering after applying, is prevented
Only sunlight irradiation and holding microbial inoculum humidity.Control group carries out conventional fertilizer application, does not apply bacteria agent.
It is calculated, which is 18.2% to the average rate of growth of tobacco leaf, and saving fertilizer rate is 55.6%.
(4) vegetables field trial
Before intensive shadow on lung sowing, vegetable nursery bed applies compound nitragin that 6kg preparation example 4 obtains as base manure per acre, turns over
It transplants seedlings after soil field planting.Control group carries out conventional fertilizer application, does not apply bacteria agent.
It is calculated, which is 20.7% to the average rate of growth of intensive shadow on lung, saves fertilizer rate and is
43.5%.
(5) rice field trial
Before rice growing, the composite bacteria agent for taking 5.5Kg preparation example 5 to obtain is dissolved in 20Kg water, dip in carry out after seedling root it is slotting
Seedling plantation.Control group carries out conventional fertilizer application, does not apply bacteria agent.
It is calculated, which is 30.5% to the average rate of growth of rice, and saving fertilizer rate is 55%.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of compound nitragin, which is characterized in that including rhizobium, vesicular-arbuscular mycorrhiza bacterium and thick spore Verticillium dahliae.
2. compound nitragin according to claim 1, which is characterized in that including nitragin, vesicular-arbuscular mycorrhiza spore
Daughter bacteria agent and thick spore Verticillium dahliae agent.
3. compound nitragin according to claim 2, which is characterized in that the compound nitragin includes by weight
The following components of number meter:
4. compound nitragin according to claim 3, which is characterized in that the compound nitragin is by parts by weight
The following components of meter forms:
5. compound nitragin according to any one of claims 1 to 4, which is characterized in that the compound nitragin
In, effective bacterium number of rhizobium is hundred million/g of 10-20, and effective bacterium number of vesicular-arbuscular mycorrhiza bacterium is hundred million/g of 1-2, thick spore wheel branch bacillus
Effective bacterium number be hundred million/g of 2-5.
6. the compound nitragin according to claim 2 to 4 any one, which is characterized in that the vesicular-arbuscular mycorrhiza
Spore microbial inoculum is prepared by high cell density fermentation.
7. compound nitragin according to claim 6, which is characterized in that containing following in every 1 high-density fermentation liquid
Ingredient: fructose 6g, natural plant gum 8g, glycine 0.2g, proline 0.05g, maltose 10g, magnesium sulfate 0.6g, potassium chloride 0.08g,
Inositol 0.05g, ammonium molybdate 0.0002g, niacin 0.0006g, copper sulphate 0.0002g, boric acid 0.005g, remainder are pure water.
8. compound nitragin according to claim 6, which is characterized in that the preparation process the following steps are included:
The pH value of the high density fermentation liquid is adjusted to 6.5 or so, 121 DEG C of sterilizings after twenty minutes, 25 DEG C are cooled to, by sterile
Operation access Vesicular arbuscular mycorriza spore, then at 24~26 DEG C, revolving speed is 50~80rpm/min, ventilating ratio 1:0.3
(V/Vm), tank pressure is cultivated 15 days under the conditions of being 0.03~0.05MPa, obtains the vesicular-arbuscular mycorrhiza bacterium spore microbial inoculum.
9. according to claim 1 to the preparation method of any one of 8 compound nitragins, which is characterized in that including following step
It is rapid:
Diatomite and mannitol are added into the conical twin-screw mixer of operation, then sequentially adds nitragin, thick spore wheel
Branch microbial inoculum and vesicular-arbuscular mycorrhiza spore microbial inoculum stop stirring after mixing, are then packed and stored.
10. the application of any one of claim 1 to the 8 compound nitragin in agricultural production.
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| CN111280188B (en) * | 2020-02-12 | 2021-04-27 | 河北环境工程学院 | Composition for promoting leguminous plant root nodule generation and method for promoting leguminous plant root nodule generation |
| CN116199538A (en) * | 2022-11-30 | 2023-06-02 | 乐施康农业科技有限公司 | Microbial agent for promoting peanut growth and preparation method thereof |
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