CN107778095A - A kind of environment-friendly type maize straw fertilizer and preparation method thereof - Google Patents
A kind of environment-friendly type maize straw fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN107778095A CN107778095A CN201711304821.4A CN201711304821A CN107778095A CN 107778095 A CN107778095 A CN 107778095A CN 201711304821 A CN201711304821 A CN 201711304821A CN 107778095 A CN107778095 A CN 107778095A
- Authority
- CN
- China
- Prior art keywords
- parts
- maize straw
- environment
- fertilizer
- friendly type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of fertilizer, and in particular to a kind of environment-friendly type maize straw fertilizer and preparation method thereof, the environment-friendly type maize straw fertilizer are made up of following raw material by following weight proportions：70~80 parts of corn stalk powder；2~3 parts of calcium superphosphate；2~4 parts of Diammonium phosphate (DAP)；2~3 parts of potassium fulvate；12 parts of bacillus subtilis；1.5 3 parts of saccharomycete；8 10 parts of trace element；15 20 parts of water.Environment-friendly type maize straw fertilizer provided by the invention is the synthesis containing a variety of nutrients such as trace element, nitrogenous fertilizer, potash fertilizer and phosphate fertilizer, and the balanced various nutrition of fertilizer energy of the present invention, so that nutrient preferably absorbs, and serve the effect of regulation soil balanced nutrients.A kind of preparation method for environment-friendly type maize straw fertilizer that the present invention also provides, this method is simple to operate, and obtained fertilizer can make crop yield, environmentally safe, energy improved soil physicochemical character.
Description
Technical field
The present invention relates to a kind of fertilizer, and in particular to a kind of environment-friendly type maize straw fertilizer and preparation method thereof.
Background technology
Microbial manure refers to a kind of microorganism containing work and can obtain specific fertilizer effect in use, can increase plant
Produce amount or the biological agent for improving quality.Many national agricultural experts test proof for many years in the world, use legume inoculation
The legumes such as soybean, peanut can improve symbiotic nitrogen fixation efficiency, there is effect of increasing production really, rationally dress seed or apply using other bacterial manure
With microbial manure, also there is effect of increasing production to non-legume crops, and the effect for thering is chemical fertilizer not reach.
But be often at present single making microbial manure in production, although environmentally friendly, fertilizer efficiency is unsatisfactory.Also have
Small part producer is mixed microbial manure and other fertilizer, and preferably nutrient can be provided for plant, and ensure plant has
Effect growth.But with the increase of inorganic fertilizer amount of application, it is bad to there is soil texture destruction, content of organic matter decline etc.
Phenomenon.
In order to overcome above-mentioned drawback, a kind of energy activating soil is sought after in cultivation of fields, the usage amount of chemical fertilizer is reduced, reduces
Pollution to environment, the active microorganism composite fertilizer containing multiple nutritional components are sharp to improve absorption of the crops to organic fertilizer
With making crops stable and high yields and turn into nuisanceless green product.
The content of the invention
It is an object of the invention to provide it is a kind of can activating soil, reduce the usage amount of chemical fertilizer, reduce pollution to environment, contain
The active microorganism composite fertilizer for having multiple nutritional components absorbs to improve crops to organic fertilizer, make crops stable yields,
High yield simultaneously turns into nuisanceless green product, and then provides a kind of environment-friendly type maize straw fertilizer and preparation method thereof.
To achieve these goals, technical scheme is specific as follows：
A kind of environment-friendly type maize straw fertilizer, it is made up of following raw material by following weight proportions：
70~80 parts of corn stalk powder；
2~3 parts of calcium superphosphate；
2~4 parts of Diammonium phosphate (DAP)；
2~3 parts of potassium fulvate；
Bacillus subtilis 1-2 parts；
Saccharomycete 1.5-3 parts；
Micro- 8-10 parts；
Water 15-20 parts.
In the above-mentioned technical solutions, the environment-friendly type maize straw fertilizer is by following weight proportion groups by following raw material
Into：
70 parts of corn stalk powder；
3 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1 part of bacillus subtilis；
2 parts of saccharomycete；
10 parts of trace element；
20 parts of water.
In the above-mentioned technical solutions, the environment-friendly type maize straw fertilizer is by following weight proportion groups by following raw material
Into：
75 parts of corn stalk powder；
3 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1.5 parts of bacillus subtilis；
1.5 parts of saccharomycete；
8 parts of trace element；
15 parts of water.
In the above-mentioned technical solutions, the environment-friendly type maize straw fertilizer is by following weight proportion groups by following raw material
Into：
70 parts of corn stalk powder；
2 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1 part of bacillus subtilis；
1.5 parts of saccharomycete；
8 parts of trace element；
15 parts of water.
In the above-mentioned technical solutions, the environment-friendly type maize straw fertilizer is by following weight proportion groups by following raw material
Into：
80 parts of corn stalk powder；
3 parts of calcium superphosphate；
4 parts of Diammonium phosphate (DAP)；
3 parts of potassium fulvate；
2 parts of bacillus subtilis；
3 parts of saccharomycete；
10 parts of trace element；
20 parts of water.
In the above-mentioned technical solutions, the trace element is made up of copper, zinc, calcium, iron and manganese.
In the above-mentioned technical solutions, the trace element is by copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride group
Into.
A kind of preparation method of environment-friendly type maize straw fertilizer, comprises the following steps：
Step 1, culture is enlarged to bacillus subtilis and saccharomycete respectively, after press filtration, flash evaporation drying, according to withered
The weight proportion of careless bacillus and saccharomycete mixes, and mixed microorganism yeast powder is made；
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, are carried out after drying with high speed disintegrator
Crush, sieving, that is, corn stalk powder is made；
Step 3, match somebody with somebody according to the weight of corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, trace element and water
It is well mixed than weighing each raw material, and by the aforementioned base materials weighed with mixed microorganism yeast powder made from step (1), you can system
Obtain environment-friendly type maize straw fertilizer.
In the above-mentioned technical solutions, culture is enlarged to bacillus subtilis or saccharomycete to comprise the following steps that：
(1), bacillus subtilis or yeast seeds are inoculated in solid slope culture medium, in 35 DEG C of activation cultures
32-38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, in 180-220r/min rotating speed bar
Under part, 35 DEG C of shaken cultivation 32-38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved
More than 45%, tank pressure 0.45Mpa, under the conditions of 32-38 DEG C of temperature, seed tank culture 35h, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 32-38 DEG C of temperature, bacillus subtilis or saccharomycete is made
Zymocyte liquid；
The composition of the fluid nutrient medium is：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef
Cream+20g agar.
The beneficial effects of the invention are as follows：
Environment-friendly type maize straw fertilizer provided by the invention using this renewable resource of maize straw as fertilizer source,
The kind of fertilizer is added, expands the source of fertilizer.On the one hand the advantage that maize straw source is wide, stock number is big is make use of,
On the other hand using vegetable fertilizer source to soil nutrient, soil physico-chemical property and soil fertility have the notable spy of improving effect
Point, the chemically composited fertilizer in part is substituted, the effect of moisturizing and loose soil property are played in soil, changes soil hardening, hardening etc.
Phenomenon, agricultural sustainable development is realized, reduce soil pollution, and environmental protection.Bacillus subtilis used and saccharomycete it is mixed
Microbial manure can be provided for plant by closing microbial inoculum, and bacillus subtilis microbial inoculum can effectively prevent and treat the powdery mildew of plant, rice blast, red
Mildew, banded sclerotial blight, anthracnose, downy mildew, bacterial wilt, gray mold etc., while soil texture can be promoted to be formed, improve hole shape
Condition；The cationic absorbent performance of soil is improved, increases the fertilizer supply and preservation ability of soil；Strengthen soil buffer system, improvement is acid
Soil；Promote the activity of soil beneficial microorganism, while play a part of increasing production, increase income, growth promoting, raising yield；Product can be improved
Matter, high-yield early-maturing, improve pol, improve ground temperature；Can be improved the soil fertility, retain water and nutrients, formation soil granular structure, improved soil
Physicochemical character；Cold-resistant, drought resisting, disease-resistant, degeneration-resistant effect can be played, so as to greatly improve the survival rate of plant.
Environment-friendly type maize straw fertilizer provided by the invention is containing a variety of nutrients such as trace element, nitrogenous fertilizer, potash fertilizer and phosphate fertilizer
Synthesis, and the fertilizer of the present invention can balanced various nutrition so that nutrient preferably absorbs, and serves regulation
The effect of soil balanced nutrients.
Environment-friendly type maize straw fertilizer provided by the invention becomes Activities of Some Plants rubbish using corn stalk powder as primary raw material
Waste be changed into values, this part of resource of effective recycling, can improved soil environment, reduce environmental pollution and pressure.
The preparation method of environment-friendly type maize straw fertilizer provided by the invention, it is simple to operate, and also obtained fertilizer can make
Crop yield, environmentally safe, energy improved soil physicochemical character.
Embodiment
The present invention provides a kind of environment-friendly type maize straw fertilizer, is made up of following raw material by following weight proportions：
70~80 parts of corn stalk powder；2~3 parts of calcium superphosphate；2~4 parts of Diammonium phosphate (DAP)；2~3 parts of potassium fulvate；Withered grass
Bacillus 1-2 parts；Saccharomycete 1.5-3 parts；Micro- 8-10 parts；Water 15-20 parts.
Preferably：The environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：Maize straw
It is 75 parts of powder, 3 parts of calcium superphosphate, 2 parts of Diammonium phosphate (DAP), 2 parts of potassium fulvate, 1.5 parts of bacillus subtilis, 1.5 parts of saccharomycete, micro
8 parts of element, 15 parts of water；Or the environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：Corn stalk
It is 70 parts of stalk powder, 2 parts of calcium superphosphate, 2 parts of Diammonium phosphate (DAP), 2 parts of potassium fulvate, 1 part of bacillus subtilis, 1.5 parts of saccharomycete, micro
8 parts of element, 15 parts of water；Or the environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：Corn stalk
80 parts of stalk powder, 3 parts of calcium superphosphate, 4 parts of Diammonium phosphate (DAP), 3 parts of potassium fulvate, 2 parts of bacillus subtilis, 3 parts of saccharomycete, micro member
Plain 10 parts, 20 parts of water.Most preferably described environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：Corn
70 parts of powder of straw；3 parts of calcium superphosphate；2 parts of Diammonium phosphate (DAP)；2 parts of potassium fulvate；1 part of bacillus subtilis；2 parts of saccharomycete；It is micro
10 parts of element；20 parts of water.
It is preferred that the trace element is made up of copper, zinc, calcium, iron and manganese.Most preferably described trace element is by copper molybdate, nitric acid
Zinc, line borate, ferric sulfate and manganese chloride composition.
The effect of part material used, is as follows：
Potassium fulvate：Potassium fulvate is a kind of pure natural mineral matter activity potassium element fertilizer, potassium fulvate include trace element,
The multiple nutritional components such as rare earth element, plant growth regulator, viral inhibitors, make nutrient it is more sufficient, supply it is more reasonable, so as to
Avoid crop because lack element and caused by various physiological disturbances generation, make the more vigorous leaf color of crop plant type more dark green,
Lodging tolerance is stronger.Potassium fulvate can timely supplement the nutrient being lost in soil, make soil activating, have vitality,
Reduce nutrient continuous cropping disease caused by taken in excess in soil, product can replace content identical potassium sulfate or chlorine completely
Change potassium and potassium magnesium sulfate, and naturally, environmental protection.
Saccharomycete：Saccharomycete (Saccharomyces) is fungal organism, more chaotic in classification, mainly because of its form
Caused by differing.By JLodder yeast classification, the Saccharomycetaceae (Saccha of the category Ascomycetes of ascospore can be formed
Romycetaceae), also referred to as true yeast such as Dbaly yeast (SDebaryomyces).Also some yeast do not form spore, category
In Fungi imperfecti, desert stalk spore mesh, Cryptococcaceae (Cryptococcaceae), such as Candida (Candidaspp).Yeast
Bacterium typically has very high nutritive value, particularly contains compared with polyprotein, many B family vitamins, nucleic acid and mineral matter, while
Some healthcare function active materials can be produced.Vitamin B complex can control the metabolic function of human body, keep normal effects on neural system.
Vitamin B2 and vitamin B6 are critically important vitamin to skin.Vitamin B12 prevents anaemia, and has promotion intestines
The effect of interior vitamin synthesis, so the people not strong to intestines or liver function has the effect of enhancing muscle power.In addition, someone reports, ferment
Female bacterium SH2 fermentation culture mediums are analyzed with gel chromatography column G-75, when Protein elution separation is carried out at 280nm wavelength, are found
There are 2 Protein elution peaks to have humidification to interferon potency wherein, and then prove that this thing is that (albumen is nucleoprotein than sugar
3：1), but it is not DNA or RNA.If this experiment information is further confirmed, saccharomycetes to make fermentation culture can be explained
There is enhancing interferon potency, so as to there is enhancing body's immunity.
Bacillus subtilis：Caused subtilin, polymyxins, mildew making during bacillus subtilis thalli growth
Element, gramicidins isoreactivity material, these active materials have obvious suppression to the conditioned pathogen of pathogenic bacteria or autogenous infection
Make and use.Bacillus subtilis consumes rapidly the free oxygen in environment, causes enteron aisle hypoxemia, promotes beneficial to anaerobism bacteria growing, and
The organic acids such as lactic acid are produced, enteron aisle pH value is reduced, suppresses other pathogenic bacteria growings indirectly.The growth of stimulating animal immune organ
Development, activation T, bone-marrow-derived lymphocyte, improves immunoglobulin and antibody level, strengthens cellular immunity and humoral immune function, improves
Herd immunity.The enzymes such as bacillus subtilis thalline itself synthesis alpha-amylase, protease, lipase, cellulase, are disappearing
Change and together played a role with the digestive enzymes in animal body in road, a variety of B races dimension lifes such as vitamin B1, B2, B6, nicotinic acid can be synthesized
Element, improve the activity of interferon and macrophage in animal body.
Zinc：Content of the zinc in object is made, generally the 100000 of dry/several to a few millionths.In crop organ
In, with terminal bud zinc content highest, leaf takes second place, and stem is minimum.Zinc is the shape of the constituent of a variety of enzymes in plant, zinc and chlorophyll
Into relevant, as crop zinc-deficiency can cause leaf chlorosis, zinc also has to cold resistance, heat resistance, drought tolerance, the salt-resistance for strengthening crop
Good effect.Application of zinc fertilizer can promote crop growth, change the ratio of seed and stalk, increase the economic production of crop
Amount.Zinc has mobility in plant, shows the tender organ of children more, and crop zinc-deficiency is shown as more：Plant is small, leaf chlorosis, there is ash
Green or yellowish-white spot, root growth are undeveloped.Application of zinc fertilizer volume increase significantly has：Corn, rice, cotton, wheat, beet, Portugal
Grape etc..Experiment and demonstration shows that zinc fertilizer can not only improve crop yield, and can significantly improve crop quality.
Manganese：Distribution of the manganese in crop organ, it is generally most with blade, it is seed again next to that stalk, green portion
More than non-green part.Manganese is the constituent of chloroplaset, is to maintain nutrient necessary to chloroplast structure, can promote crop
Photosynthesis.Cotton applies manganese fertilizer, not only mitigates fruit abscission rate phenomenon, and make harvest one-level unginned cotton significantly increases earlier
It is more.Manganese can not recycle in crop body, and plant manganese deficiency symptom shows spire first：The chlorophyll of blade is reduced, vein it
Between chlorosis, and vein and vein nearby remain in that green.
Boron element：Boron element is not the constituent of various organic matters in crop body, but can strengthen some important lifes of crop
Manage function.Major function has：1. cell elongation and tissue is promoted to break up.2. accelerate enzyme metabolism and lignin is formed.3. carbon aquation
Compound transports and protein metabolism.4. promote root growth and development.5. strengthen crop anti-adversity.6. promote early ripening of the crops modification.
7. promote pollen germination and pollen tube growth.
The boron content of Different Crop is widely different, and dicotyledonous crops are significantly higher than monocot crops, wherein made with pulse family again
Thing and crop in cruciferae highest, and gramineous crop is minimum.In crop Different Organs, the resultant of boron element is also different, leaf
The content highest of son, flower take second place, and minimum in the organ such as stem, root, fruit, seed.Boron can strengthen the photosynthesis of crop, promote
The normal operation of carbohydrate, promote the normal development of crop propagating organ, be advantageous to blossom and bear fruit.Using appropriate boron fertilizer
Floral development can be accelerated, increase pollen quantity, promote the sprouting of pollen grain and the growth of pollen tube.
Crop boron deficiency apical growing point is abnormal or growth arrest, spire deformity, shrinkage, chlorosis between vein, lower blade
Thicken, leaf color is deepened, and plant is short and small.
Molybdenum：Nitrogen metabolism is mainly influenceed in plant, tool promotes nitrogen fixation.Crop molybdenum, which lacks, generally to be shown：
Blade edge it is dried-up curling circlewise, cup-shaped.Crop different symptoms are also different, and cotton lacks molybdenum, branch point vein chlorosis, fruit abscission rate
Seriously；Wheat lacks molybdenum, and leaf chlorosis, grouting is poor, and in ripe evening, seed is not plump.
Iron：Although iron is not the composition of chlorophyll, it during ferro element subalimentation, can be hindered the synthesis of chlorophyll
Hinder, chlorosis phenomenon occurs for blade, influences the formation of photosynthesis and carbohydrate.Because iron can not be sharp once again in crop body
With therefore symptom is since spire, and during crop iron deficiency, mainly chlorophyll is destroyed, chlorosis between vein, pear tree iron deficiency, blade
Chlorosis yellow.
The present invention also provides a kind of preparation method of environment-friendly type maize straw fertilizer, comprises the following steps：
Step 1, culture is enlarged to bacillus subtilis and saccharomycete respectively, after press filtration, flash evaporation drying, according to withered
The weight proportion of careless bacillus and saccharomycete mixes, and mixed microorganism yeast powder is made；
Culture is enlarged to bacillus subtilis or saccharomycete to comprise the following steps that：
(1), bacillus subtilis or yeast seeds are inoculated in solid slope culture medium, in 35 DEG C of activation cultures
32-38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, in 180-220r/min rotating speed bar
Under part, 35 DEG C of shaken cultivation 32-38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved
More than 45%, tank pressure 0.45Mpa, under the conditions of 32-38 DEG C of temperature, seed tank culture 35h, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 32-38 DEG C of temperature, bacillus subtilis or saccharomycete is made
Zymocyte liquid；
The composition of the fluid nutrient medium is：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef
Cream+20g agar.
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, are carried out after drying with high speed disintegrator
Crush, sieving, that is, corn stalk powder is made；
Step 3, match somebody with somebody according to the weight of corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, trace element and water
It is well mixed than weighing each raw material, and by the aforementioned base materials weighed with mixed microorganism yeast powder made from step (1), you can system
Obtain environment-friendly type maize straw fertilizer.
Example 1,
The environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：70 parts of corn stalk powder,
3 parts of calcium superphosphate, 2 parts of Diammonium phosphate (DAP), 2 parts of potassium fulvate, 1 part of bacillus subtilis, 2 parts of saccharomycete, micro- (molybdic acid
Copper, zinc nitrate, line borate, ferric sulfate and manganese chloride) 10 parts, the copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride
Weight proportion be 1:3:1:1:1.5,20 parts of water；
Step 1, bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces) are carried out respectively
Expand culture, after press filtration, flash evaporation drying, mixed by above-mentioned weight proportion, mixed microorganism yeast powder is made；
Bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces) be enlarged culture by
Following steps operate：
(1), strain is inoculated in solid slope culture medium, in 35 DEG C of activation culture 32h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, under 180r/min speed conditions,
35 DEG C of shaken cultivation 32h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 35h under the conditions of 32 DEG C of temperature, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 32 DEG C of temperature, zymocyte liquid is made.
The composition of culture medium：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g fine jades
Fat.
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, high speed disintegrator is carried out after drying and is entered
Row crushes, sieving.Corn stalk powder is made.
Step 3, according to corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, micro- (copper molybdate, nitric acid
Zinc, line borate, ferric sulfate and manganese chloride) and the weight proportion of water weigh each raw material, and by the aforementioned base materials weighed and step (1)
Obtained mixed microorganism yeast powder is well mixed, you can environment-friendly type maize straw fertilizer is made.
Example 2,
The environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：75 parts of corn stalk powder,
3 parts of calcium superphosphate, 2 parts of Diammonium phosphate (DAP), 2 parts of potassium fulvate, 1.5 parts of bacillus subtilis, 1.5 parts of saccharomycete, micro- (molybdenum
Sour copper, zinc nitrate, line borate, ferric sulfate and manganese chloride) 8 parts, the copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride
Weight proportion be 1:3:1:1:2), 15 parts of water；
Step 1, bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces) are carried out respectively
Expand culture, after press filtration, flash evaporation drying, mixed by above-mentioned weight proportion, mixed microorganism yeast powder is made；
It is equal that culture is enlarged to bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces)
Operate as follows：
(1), strain is inoculated in solid slope culture medium, in 35 DEG C of activation culture 38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, under 220r/min speed conditions,
35 DEG C of shaken cultivation 38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 35h under the conditions of 38 DEG C of temperature, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 38 DEG C of temperature, zymocyte liquid is made.
The composition of culture medium：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g fine jades
Fat.
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, high speed disintegrator is carried out after drying and is entered
Row crushes, and sieving, that is, corn stalk powder is made.
Step 3, according to corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, micro- (copper molybdate, nitric acid
Zinc, line borate, ferric sulfate and manganese chloride) and the weight proportion of water weigh each raw material, and by the aforementioned base materials weighed and step (1)
Obtained mixed microorganism yeast powder is well mixed, you can environment-friendly type maize straw fertilizer is made.
Embodiment 3
The environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：70 parts of corn stalk powder,
2 parts of calcium superphosphate, 2 parts of Diammonium phosphate (DAP), 2 parts of potassium fulvate, 1 part of bacillus subtilis, 1.5 parts of saccharomycete, micro- (molybdic acid
Copper, zinc nitrate, line borate, ferric sulfate and manganese chloride) 8 parts, the copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride
Weight proportion is 1:3:1:1:2,15 parts of water；
Step 1, bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces) are carried out respectively
Expand culture, after press filtration, flash evaporation drying, mixed by above-mentioned weight proportion, mixed microorganism yeast powder is made；
It is equal that culture is enlarged to bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces)
Operate as follows：
(1), strain is inoculated in solid slope culture medium, in 35 DEG C of activation culture 38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, under 220r/min speed conditions,
35 DEG C of shaken cultivation 38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 35h under the conditions of 38 DEG C of temperature, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 38 DEG C of temperature, zymocyte liquid is made.
The composition of culture medium：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g fine jades
Fat.
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, high speed disintegrator is carried out after drying and is entered
Row crushes, and sieving, that is, corn stalk powder is made.
Step 3, according to corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, micro- (copper molybdate, nitric acid
Zinc, line borate, ferric sulfate and manganese chloride) and the weight proportion of water weigh each raw material, and by the aforementioned base materials weighed and step (1)
Obtained mixed microorganism yeast powder is well mixed, you can environment-friendly type maize straw fertilizer is made.
Embodiment 4
The environment-friendly type maize straw fertilizer is made up of following raw material by following weight proportions：80 parts of corn stalk powder,
3 parts of calcium superphosphate, 4 parts of Diammonium phosphate (DAP), 3 parts of potassium fulvate, 2 parts of bacillus subtilis, 3 parts of saccharomycete, micro- (molybdic acid
Copper, zinc nitrate, line borate, ferric sulfate and manganese chloride) 10 parts, the copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride
Weight proportion be 1.5:4:1.5:1:2,20 parts of water；
Step 1, bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces) are carried out respectively
Expand culture, after press filtration, flash evaporation drying, mixed by above-mentioned weight proportion, mixed microorganism yeast powder is made；
It is equal that culture is enlarged to bacillus subtilis (Bacillus subtilis), saccharomycete (Saccharomyces)
Operate as follows：
(1), strain is inoculated in solid slope culture medium, in 35 DEG C of activation culture 38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, under 220r/min speed conditions,
35 DEG C of shaken cultivation 38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 35h under the conditions of 38 DEG C of temperature, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
More than 45%, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 38 DEG C of temperature, zymocyte liquid is made.
The composition of culture medium：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g fine jades
Fat.
Step 2, after maize straw is dried, 60 DEG C of drying are carried out into drying tower, high speed disintegrator is carried out after drying and is entered
Row crushes, and sieving, that is, corn stalk powder is made.
Step 3, according to corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, micro- (copper molybdate, nitric acid
Zinc, line borate, ferric sulfate and manganese chloride) and the weight proportion of water weigh each raw material, and by the aforementioned base materials weighed and step (1)
Obtained mixed microorganism yeast powder is well mixed, you can environment-friendly type maize straw fertilizer is made.
The field experiment of embodiment 5：
1st, experiment material
It is land mine watermelon for examination variety of watermelon.
Experimental group：The compound microorganism ferments organic fertilizer prepared using 1-4 of the embodiment of the present invention.
Control group：Using common farm manure.
2nd, experimental method
2.1 experimental design
Test and carried out in 2016 in Yushu City agricultural technology extension center, be divided into the progress of 5th area, wherein 4th area are experiment
Area, it is designated as testing 1st area, 2nd area of experiment, 3rd area of experiment, 4th area of experiment respectively, 1st area is check plot.1-4 areas are wherein tested to apply respectively
Compound microorganism ferments organic fertilizer prepared by 1-4 of the embodiment of the present invention, common farm manure is applied in check plot.By point of various fertilizer
Watermelon Yong Yu not be planted, the amount of application of various fertilizer is identical (the amount of application administration according to 100 kgs/acre), and planting density is identical,
100 watermelons are planted respectively.The result of the test of various fertilizer is as shown in table 1：
Table 1：Plantation watermelon applies different fertilizer contrast table
| Test 1st area | Test 2nd area | Test 3rd area | Test 4th area | Check plot | |
| Using fertilizer | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Common farm manure |
| Survival rate | 99% | 98% | 96% | 97% | 80% |
| Blade condition | It is excellent | It is excellent | It is excellent | It is excellent | It is good |
| Receive watermelon number | 2200 | 2150 | 2100 | 2120 | 1750 |
| Yield kg/ mus | 4230 | 4185 | 4150 | 4130 | 2980 |
As shown in Table 1：From the survival rate of watermelon, blade condition, receive watermelon number and per mu yield relatively from the point of view of, test block is equal
Better than check plot, and the effect of embodiment 1 is best.Using environment-friendly type maize straw fertilizer provided by the invention plantation watermelon with applying
Common farm nanure planting watermelon is compared, and its per mu yield highest can improve 41.9%.
Also, environment-friendly type maize straw fertilizer provided by the invention is a variety of containing trace element, nitrogenous fertilizer, potash fertilizer and phosphate fertilizer etc.
The synthesis of nutrient, and the balanced various nutrition of fertilizer energy of the present invention so that nutrient preferably absorbs, and serves
Adjust the effect of soil balanced nutrients.
Environment-friendly type maize straw fertilizer provided by the invention becomes Activities of Some Plants rubbish using corn stalk powder as primary raw material
Waste be changed into values, this part of resource of effective recycling, can improved soil environment, reduce environmental pollution and pressure.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of environment-friendly type maize straw fertilizer, it is characterised in that be made up of following raw material by following weight proportions：
70~80 parts of corn stalk powder；
2~3 parts of calcium superphosphate；
2~4 parts of Diammonium phosphate (DAP)；
2~3 parts of potassium fulvate；
Bacillus subtilis 1-2 parts；
Saccharomycete 1.5-3 parts；
Micro- 8-10 parts；
Water 15-20 parts.
2. environment-friendly type maize straw fertilizer according to claim 1, it is characterised in that be by following weight by following raw material
Proportioning composition：
70 parts of corn stalk powder；
3 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1 part of bacillus subtilis；
2 parts of saccharomycete；
10 parts of trace element；
20 parts of water.
3. environment-friendly type maize straw fertilizer according to claim 1, it is characterised in that be by following weight by following raw material
Proportioning composition：
75 parts of corn stalk powder；
3 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1.5 parts of bacillus subtilis；
1.5 parts of saccharomycete；
8 parts of trace element；
15 parts of water.
4. environment-friendly type maize straw fertilizer according to claim 1, it is characterised in that be by following weight by following raw material
Proportioning composition：
70 parts of corn stalk powder；
2 parts of calcium superphosphate；
2 parts of Diammonium phosphate (DAP)；
2 parts of potassium fulvate；
1 part of bacillus subtilis；
1.5 parts of saccharomycete；
8 parts of trace element；
15 parts of water.
5. environment-friendly type maize straw fertilizer according to claim 1, it is characterised in that be by following weight by following raw material
Proportioning composition：
80 parts of corn stalk powder；
3 parts of calcium superphosphate；
4 parts of Diammonium phosphate (DAP)；
3 parts of potassium fulvate；
2 parts of bacillus subtilis；
3 parts of saccharomycete；
10 parts of trace element；
20 parts of water.
6. the environment-friendly type maize straw fertilizer according to claim 1-5 any one, it is characterised in that the trace element
It is made up of copper, zinc, calcium, iron and manganese.
7. the environment-friendly type maize straw fertilizer according to claim 1-5 any one, it is characterised in that the trace element
It is made up of copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride.
8. environment-friendly type maize straw fertilizer according to claim 7, it is characterised in that the copper molybdate, zinc nitrate, boric acid
The weight proportion of calcium, ferric sulfate and manganese chloride is 1~1.5:3~4:1~1.5:1:1.5~2.
9. the preparation method of the environment-friendly type maize straw fertilizer described in claim 1-5 any one, it is characterised in that including with
Lower step：
Step 1, culture is enlarged to bacillus subtilis and saccharomycete respectively, after press filtration, flash evaporation drying, according to withered grass bud
The weight proportion of spore bacillus and saccharomycete mixes, and mixed microorganism yeast powder is made；
Step 2, after maize straw are dried, 60 DEG C of drying are carried out into drying tower, are crushed after drying with high speed disintegrator,
Sieving, that is, corn stalk powder is made；
Step 3, claim according to the weight proportion of corn stalk powder, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, trace element and water
Each raw material is taken, and the aforementioned base materials weighed are well mixed with mixed microorganism yeast powder made from step (1), you can ring is made
Guarantor's type maize straw fertilizer.
10. the preparation method of environment-friendly type maize straw fertilizer according to claim 9, it is characterised in that to withered grass gemma
Bacillus or saccharomycete are enlarged comprising the following steps that for culture：
(1), bacillus subtilis or yeast seeds are inoculated in solid slope culture medium, in 35 DEG C of activation culture 32-
38h, activated seed is made；
(2), activated seed made from step (1) is inoculated in fluid nutrient medium, under 180-220r/min speed conditions,
35 DEG C of shaken cultivation 32-38h, first order seed is made；
(3), first order seed made from step (2) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved 45%
More than, tank pressure 0.45Mpa, under the conditions of 32-38 DEG C of temperature, seed tank culture 35h, secondary seed is made；
(4), secondary seed made from step (3) is inoculated in fluid nutrient medium by percent by volume for 9%, oxygen dissolved 45%
More than, tank pressure 0.45Mpa, seed tank culture 48h under the conditions of 32-38 DEG C of temperature, bacillus subtilis or saccharomycetes to make fermentation is made
Bacterium solution；
The composition of the fluid nutrient medium is：1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extracts+
20g agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711304821.4A CN107778095A (en) | 2017-12-11 | 2017-12-11 | A kind of environment-friendly type maize straw fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711304821.4A CN107778095A (en) | 2017-12-11 | 2017-12-11 | A kind of environment-friendly type maize straw fertilizer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107778095A true CN107778095A (en) | 2018-03-09 |
Family
ID=61430071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711304821.4A Pending CN107778095A (en) | 2017-12-11 | 2017-12-11 | A kind of environment-friendly type maize straw fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107778095A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383585A (en) * | 2018-05-31 | 2018-08-10 | 江苏盛禾资源环境科技有限责任公司 | A kind of epipremnum aureum fertilizer |
| CN108863663A (en) * | 2018-07-27 | 2018-11-23 | 吉林省新农科生态农业科技有限公司 | A kind of tobacco fertilizer special for organic and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642726A (en) * | 2013-11-28 | 2014-03-19 | 天津海友佳音生物科技股份有限公司 | Corn straw fermentation microorganism agent and preparation method and fermentation method thereof |
| CN106631513A (en) * | 2017-01-09 | 2017-05-10 | 漯河市兴捷生物科技有限公司 | Corn or wheat straw converted organic bacterial manure and preparation method thereof |
-
2017
- 2017-12-11 CN CN201711304821.4A patent/CN107778095A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642726A (en) * | 2013-11-28 | 2014-03-19 | 天津海友佳音生物科技股份有限公司 | Corn straw fermentation microorganism agent and preparation method and fermentation method thereof |
| CN106631513A (en) * | 2017-01-09 | 2017-05-10 | 漯河市兴捷生物科技有限公司 | Corn or wheat straw converted organic bacterial manure and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 赵丹宁: "《百度百科》", 24 April 2017 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383585A (en) * | 2018-05-31 | 2018-08-10 | 江苏盛禾资源环境科技有限责任公司 | A kind of epipremnum aureum fertilizer |
| CN108863663A (en) * | 2018-07-27 | 2018-11-23 | 吉林省新农科生态农业科技有限公司 | A kind of tobacco fertilizer special for organic and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105294284B (en) | A kind of preparation method of stalk fermentation bio-feritlizer | |
| CN102910988B (en) | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer | |
| CN101914447B (en) | Complex microbial inoculum 707 and preparation method and application thereof | |
| AU2008331378B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN103571766B (en) | Liquid microbial leaf fertilizer, and production method and applications thereof | |
| CN102229510B (en) | Formula of microbial composite fertilizer | |
| CN109485498A (en) | A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer | |
| CN107043280A (en) | A kind of biological compound fertilizer and preparation method thereof | |
| CN102491853A (en) | High-efficiency hardening-resisting continuous-cropping-resisting soil-modifying biological agent | |
| CN101974428A (en) | Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof | |
| CN105481580A (en) | Novel organic fertilizer for kiwifruits and preparation method thereof | |
| CN103772011B (en) | The liquid bacterial manure of a kind of cereal special composite biological | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN104130068B (en) | A kind of composite multi-functional biological foliage fertilizer | |
| CN104788237B (en) | A kind of wheat selenium-rich zinc-iron microbial fermentation fertilizer and its preparation method and application | |
| CN108017446A (en) | The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium | |
| CN108863550A (en) | A kind of dedicated disease prevention growth-promoting composite microbic bacterial fertilizer of tea tree and preparation method thereof | |
| CN112159776B (en) | Complex enzyme preparation for improving fertility of cultivated land and preparation method thereof | |
| CN107324940A (en) | A kind of dragon fruit synergy fertilizer and preparation method thereof | |
| CN109928794A (en) | A kind of selenium-rich passion fruit fertilizer special for organic and preparation method thereof | |
| CN105198512B (en) | A kind of method that double-microbial fermentation prepares gamma-polyglutamic acid organic fertilizer | |
| CN107778095A (en) | A kind of environment-friendly type maize straw fertilizer and preparation method thereof | |
| CN108821858A (en) | The disease-resistant dedicated bacterial manure of dragon fruit, preparation method and applications | |
| CN104817367A (en) | Preparation method of selenium-rich special biological organic fertilizer for cauliflower | |
| CN104926457B (en) | A kind of potato selenium-rich zinc-iron microbial fermentation fertilizer and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180309 |