CN108863663A - A kind of tobacco fertilizer special for organic and preparation method thereof - Google Patents
A kind of tobacco fertilizer special for organic and preparation method thereof Download PDFInfo
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- CN108863663A CN108863663A CN201810847699.3A CN201810847699A CN108863663A CN 108863663 A CN108863663 A CN 108863663A CN 201810847699 A CN201810847699 A CN 201810847699A CN 108863663 A CN108863663 A CN 108863663A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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Abstract
A kind of tobacco fertilizer special for organic and preparation method thereof, is related to fertilizer field, solves the problems, such as that existing tobacco-growing soil chronic administration chemical fertilizer causes soil organic matter content to fall sharply, soil physical property deteriorates to influence the quality and yield of tobacco leaf.A kind of tobacco fertilizer special for organic of the invention includes following component according to parts by weight：55~60 parts of corn stover；2~3 parts of calcium superphosphate；1~2 part of Diammonium phosphate (DAP)；10~15 parts of potassium fulvate；1~2 part of bacillus subtilis；1.5~3 parts of saccharomycete；8~10 parts of trace element mixture；15~20 parts of water；Trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride.Many advantages, such as tobacco fertilizer special for organic of the invention has simple process compared with traditional organic fertilizer, and raw material sources are extensive, low in cost, and fertilizer efficiency is good, and cigarette quality is good, tobacco production is high.
Description
Technical field
The present invention relates to technical field of fertilizers, and in particular to a kind of tobacco fertilizer special for organic and preparation method thereof.
Background technique
Tobacco (scientific name：Nicotiana tabacum L.) belong to tubular flower subject, Solanaceae is annual or limited perennial grass
This plant, base portion slightly lignifying.Inflorescence basidixed, it is coniform, it spends more；Capsule ovate or square round shape, length are approximately equal to persistent calyx.Summer and autumn
Season yields positive results.It is distributed mainly on South America, South Asia, China.Some islands of the tobacco originating from U.S. state, Oceania and the South Pacific Ocean
Small island.66 kinds are had now been found that, by only 2 kinds of culture and utility, i.e. Nicotiana tabacum (N.tabacum.L.) is called safflower cigarette
Grass and makhorka (Nrustica L.).
Tobacco is aerobic more plant, maintains root function by oxygen.Soil permeability and structural and determine oxygen
Supply situation.Since nicotine is especially formed at the positions such as young root and Gen Mao in tobacco root, keep good soil logical
Gas is conducive to the synthesis of nicotine.Soil aeration is bad, then soil is obstructed for hypoxgia, root system respiration, the formation of new root
It will be reduced with activity, directly affect nicotine synthesis.Soil aeration situation and water regime have an effect on absorption of the plant to nutrient.
It ventilates good environment, root oxygen supply can be made in order, and the CO that breathing can be promoted to generate2It scatters and disappears from rhizosphere.Rhizosphere soil
The content of middle oxygen is high, can promote the aerobic respiration of cigarette strain root system, be conducive to absorption of the plant to nutrient.Moisture is raw to cigarette strain
It is long, especially have a significant impact to the growth of root system, also the same absorption for influencing nutrient indirectly.Tobacco is to K+Use have one
Provisioning request.To K+For, water shortage can both reduce it from grogs to the migration rate of root table, can also weaken the absorbability of root system.
Ground moistening is kept, alleviating During Flooding And Draining Alternating Process is to improve K+The important measures of content validity.
With the continuous improvement of people's living standards, the smoking of harmful health of people will receive a variety of severeer limits surely
System.Purposes of the tobacco in terms of cigarette can greatly weaken surely.But tobacco is many latent in terms of developing food products and drug resource
It has been found to and utilizes in purposes, future is long-range.Tobacco will not still lose the personal value of Important Economic crop.Tobacco leaf is rich
Containing protein, for flue-cured tobacco 10% or so, suncured tabacco and burley tobaccos may be up to 20%.Tobacco power of regeneration is strong, can repeatedly receive within 1 year
It obtains, yield of tobacco is high, extracts albumen using fresh tobacco leaf, per mu yield can be more than soybean, and it is even more impossible to compare for other crops.Tobacco leaf mentions
The albumen taken can make numerous food, there is extensive use, and remaining tobacco leaf can still function as the raw material of cigarette.Cigarette is extracted from tobacco leaf
Medicine, which is made, in alkali can prevent and treat the sufferer of people, and pesticide, which is made, can prevent and treat crop pests, and prospect is also considerable.Tobacco is to write
The mode crop of name, can be used as bioreactor for the anticancer of other crops, anti-AIDS and the base for being beneficial to health of people
Because import tobacco, give full expression to it, then extracted using biotechnology, can be processed into promote health and cure diseases " miracle is wonderful
Medicine ".If American scientist successfully turns out antibody tobacco, therefrom extract anticancer and it is antiviral it is dry scratch element, have to lung cancer good
Good therapeutic effect.Human body gene is injected tobacco plant by Sweden scientist, proposes that hematoglobin protein is living from the tobacco leaf of harvest
Agent can cure heart disease etc..It is therefore seen that the wholesome potential use of tobacco is unlike the economy risen as cigarette
It acts on inferior.
Chinese tobacco-growing soil unilateral attention chemical fertilizer for a long time, causes soil organic matter content to fall sharply, soil physical property
Deteriorate, the especially decline of aggregate quality and quantity, soil aeration situation is degenerated.Research has shown that the physical behavior of soil to cigarette
The quality and yield of leaf has an important influence, wherein controlling major part especially with the balance between aeration status and water regime
Quality element.And fertilizer used in agricultural production at present is mostly organic fertilizer and chemical fertilizer.Lavishment chemical fertilizer gives agricultural production band
A series of adverse effects are carried out, for example soil nutrient is out of proportion, assessing index, permeability is deteriorated, humus content drop
Low, soil hardening is serious, and so as to cause disease-resistant crops worm decline, ecological environmental pollution is on the rise.
Summary of the invention
Soil organic matter content is caused to fall sharply in order to solve existing tobacco-growing soil chronic administration chemical fertilizer, soil physical property
Shape deteriorates the problem of influencing the quality and yield of tobacco leaf, and the present invention provides a kind of tobacco fertilizer special for organic and its preparation side
Method.
Used technical solution is as follows in order to solve the technical problem by the present invention：
A kind of tobacco fertilizer special for organic of the invention includes following component according to parts by weight：
55~60 parts of corn stover；2~3 parts of calcium superphosphate；1~2 part of Diammonium phosphate (DAP)；10~15 parts of potassium fulvate；Withered grass
1~2 part of bacillus；1.5~3 parts of saccharomycete；8~10 parts of trace element mixture；15~20 parts of water；
The trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride, copper molybdate, nitric acid
Weight ratio between zinc, line borate, ferric sulfate and manganese chloride is 1:1.3:1.5:2:1.5.
As preferred embodiment, a kind of tobacco fertilizer special for organic of the invention, includes with the following group according to parts by weight
Part：
58 parts of corn stover；2.5 parts of calcium superphosphate；1.5 parts of Diammonium phosphate (DAP)；13 parts of potassium fulvate；Bacillus subtilis 1.5
Part；2 parts of saccharomycete；9 parts of trace element mixture；18 parts of water；
The trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride.
As preferred embodiment, effective bacterium number in the bacillus subtilis and saccharomycete is 8~1,000,000,000
Head/m.
The present invention also provides a kind of preparation methods of tobacco fertilizer special for organic, include the following steps：
Step 1: bacillus subtilis and saccharomycete are expanded culture respectively, after filters pressing, flash evaporation drying, according to
Proportion is uniformly mixed, and mixed microorganism yeast powder is made；
Step 2: drying after corn stover is dried, drying temperature is 50~70 DEG C, is crushed after drying, mistake
Corn stalk powder is made in sieve；
Step 3: by corn stalk powder and peroxophosphoric acid made from mixed microorganism yeast powder made from step 1, step 2
Calcium, Diammonium phosphate (DAP), potassium fulvate, copper molybdate, zinc nitrate, line borate, ferric sulfate, manganese chloride and water are uniformly mixed, and tobacco is made
Fertilizer special for organic.
As preferred embodiment, in step 2, drying temperature is 60 DEG C.
As preferred embodiment, in step 1, bacillus subtilis and saccharomycete are expanded culture specific
Process is as follows：
Step 1: strain is inoculated in solid slope culture medium, 32~38h of activation culture under the conditions of 35 DEG C, it is made
Activated seed；
It is 180~220r/ in revolving speed Step 2: activated seed made from step 1 is inoculated in fluid nutrient medium
Under conditions of min, temperature are 35 DEG C, first order seed is made in 32~38h of shaken cultivation；
Step 3: first order seed made from step 2 is inoculated in fluid nutrient medium in the ratio that percent by volume is 9%
In, 45% or more oxygen dissolved, tank presses 0.45Mpa, seed tank culture 35h under the conditions of 32~38 DEG C, and secondary seed is made；
Step 4: secondary seed made from step 3 is inoculated in fluid nutrient medium in the ratio that percent by volume is 9%
In, 45% or more oxygen dissolved, tank presses 0.45Mpa, seed tank culture 48h under the conditions of 32~38 DEG C, and zymocyte liquid is made.
As preferred embodiment, the component and dosage of the solid slope culture medium are as follows：1L distilled water；The Portugal 20g
Grape sugar；15g peptone；5g sodium chloride；0.5g beef extract；20g agar.
As preferred embodiment, the component and dosage of the fluid nutrient medium are as follows：Casein peptone 17g；Soybean egg
White peptone 3g；Sodium chloride 5g；Dipotassium hydrogen phosphate 2.5g；Glucose 2.5g；Lecithin 1g；Tween 80 7g；The fluid nutrient medium
PH is 7.2 ± 0.2.
The beneficial effects of the invention are as follows：The present invention uses corn stover, calcium superphosphate, Diammonium phosphate (DAP), potassium fulvate, withered grass
A kind of tobacco fertilizer special for organic is made in bacillus, saccharomycete, trace element mixture etc..Tobacco fertilizer special for organic of the invention
Compared with traditional organic fertilizer, there is simple process, raw material sources are extensive, and low in cost, fertilizer efficiency is good, and cigarette quality is good, tobacco produces
Measure many advantages, such as high.Tobacco fertilizer special for organic is applied, soil aeration can greatly be improved, be conducive to the synthesis of nicotine.
Specific embodiment
A kind of tobacco fertilizer special for organic of the invention includes following component according to parts by weight：
55~60 parts of corn stover；2~3 parts of calcium superphosphate；1~2 part of Diammonium phosphate (DAP)；10~15 parts of potassium fulvate；Withered grass
1~2 part of bacillus；1.5~3 parts of saccharomycete；8~10 parts of trace element mixture (copper, zinc, calcium, iron, manganese)；Water 15~20
Part.
Trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride, copper molybdate, zinc nitrate,
Weight ratio between line borate, ferric sulfate and manganese chloride is 1:1.3:1.5:2:1.5.
Effective bacterium number in bacillus subtilis and saccharomycete is 8~1,000,000,000/mL.
The component and dosage of solid slope culture medium are as follows：1L distilled water；20g glucose；15g peptone；5g chlorination
Sodium；0.5g beef extract；20g agar.
The component and dosage of fluid nutrient medium are as follows：Casein peptone 17g；Soy peptone 3g；Sodium chloride 5g；Phosphoric acid hydrogen two
Potassium 2.5g；Glucose 2.5g；Lecithin 1g；Tween 80 7g；The pH of the fluid nutrient medium is 7.2 ± 0.2.
Corn stover：Dry matter 88.8%, 3.47 thermies/kg of total energy, crude fibre 33.4%, calcium 0.31%, phosphorus 0.1%.
Lysine 0.06%, methionine 0.03%, cystine 0.03%, stalk passes through microbial action in the soil, slowly decomposes, releases
Release minerals nutrient therein, for crop absorption utilization；Organic matter, the humus formed when decomposition can be again micro- life in soil
Object and geobiont provide raw material, so that soil texture be made to be effectively improved, promote soil fertility.Soil Microorganism existence
Active, the soil system ecological balance is stablized, and fertility will consolidate raising.
Calcium superphosphate：Also known as normal superphosphate, abbreviation general calcium are with sulfuric acid decomposition phosphorus ore phosphate fertilizer directly obtained.It is main
Wanting useful constituent is the hydrate Ca (H of calcium dihydrogen phosphate2PO4)2·H2O and phosphoric acid free on a small quantity, also contain dead plaster
Component (useful to a lack of sulfur soil).Calcium superphosphate contains effective P2O514%~20% (wherein 80%~95% is dissolved in water), belongs to
Water soluble quick-acting phosphate fertilizer.Grey or greyish white toner material (or particle), can directly make phosphate fertilizer.It can also make the ingredient of compound fertilizer making.
Diammonium phosphate (DAP)：Diammonium phosphate (DAP) is a kind of quick-acting fertilizer of high concentration, is suitable for various crops and soil, particularly suitable
The crop of phosphorus is needed in nitrophilous, solid content is less after dissolution, is applicable in various crops to the needs of nitrogen and phosphorus element, is particularly suitable for
The area of drought, applicable any soil and most crop need the crop of phosphorus especially suitable for happiness ammonium, make base manure or
Top dressing, it is preferably deep to apply.Curing agent as urea-formaldehyde resin adhesive, is made into 20% aqueous solution, and curing rate is most slow.It also serves as
Additive flame retardant.If a small amount of DAP is added in nature rubber latex, the magnesium ion in latex can be effectively eliminated, will not be dropped
The tensile strength of Heveatex after low-sulfur.
Potassium fulvate is a kind of pure natural minerals activity potassium element fertilizer, potassium fulvate include microelement, rare earth element,
The multiple nutritional components such as plant growth regulator, viral inhibitors, make nutrient it is more sufficient, supply it is more reasonable, so as to avoid work
Object due to lacking element caused by various physiological disturbances generation, keep the more vigorous leaf color of crop plant type more dark green, energy resistant to lodging
Power is stronger.Potassium fulvate can timely supplement the nutrient being lost in soil, make soil activating, have vitality, reduce soil
Nutrient continuous cropping disease caused by taken in excess in earth, product can replace the identical potassium sulfate of content or potassium chloride and sulphur completely
Sour potassium magnesium, and natural, environmental protection.
The subtilin that generates during bacillus subtilis (Bacillus subtilis) thalli growth, polymyxins,
Nystatin, gramicidins isoreactivity substance, these active materials have the conditioned pathogen of pathogenic bacteria or autogenous infection bright
Aobvious inhibiting effect.Bacillus subtilis consumes rapidly the free oxygen in environment, causes enteron aisle hypoxemia, promotes raw beneficial to anaerobic bacteria
It is long, and the organic acids such as lactic acid are generated, enteron aisle pH value is reduced, other pathogenic bacteria is inhibited to grow indirectly.Stimulate animal immune organ
Growth and development, activate T, bone-marrow-derived lymphocyte, improve immunoglobulin and antibody level, enhance cellular immunity and humoral immunity function
Can, improve herd immunity.Bacillus subtilis thallus itself synthesizes the enzymes such as alpha-amylase, protease, lipase, cellulase
Class plays a role together with the intracorporal digestive enzymes of animal in alimentary canal, it is a variety of to synthesize vitamin B1, B2, B6, niacin etc.
B family vitamin improves the activity of interferon and macrophage in animal body.
Saccharomycete (Saccharomyces) is fungal organism, generally has very high nutritive value, especially contains more egg
White matter, B family vitamin, nucleic acid and minerals, while can also generate some healthcare function active materials.Vitamin B complex is controllable
The metabolic function of human body keeps normal effects on neural system.Vitamin B2 and vitamin B6 are critically important vitamin to skin.Dimension
Raw element B12 prevents anaemia, and plays the role of promoting the synthesis of enteral vitamin, so the people not strong to intestines or liver function
There is the effect of enhancing physical strength.
Trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride.
1, copper molybdate：Molybdenum element mainly influences nitrogen metabolism in plant, and tool promotes nitrogen fixation.Crop molybdenum lacks
Usually show：Blade edge it is dried-up curling circlewise, cup-shaped.Crop different symptoms are also different, and cotton lacks molybdenum, and branch point vein loses
Green, fruit abscission rate is serious；Wheat lacks molybdenum, and leaf chlorosis, it is poor to be in the milk, and in mature evening, seed is not plump.
2, zinc nitrate：Zn-ef ficiency is making the content in object, generally the 100000 of dry matter/several to a few millionths.
In crop organ, with terminal bud zinc content highest, leaf takes second place, and stem is minimum.Zinc is the constituent of a variety of enzymes in plant, zinc and
The formation of chlorophyll is related, and if crop zinc-deficiency can cause leaf chlorosis, zinc is also to the cold resistance of enhancing crop, heat resistance, drought-enduring
Property, salt-resistance have good effect.Application of zinc fertilizer can promote crop growth, change the ratio of seed and stalk, increase and make
The economic flow rate of object.Zinc has mobility in plant, and the tender organ of children, crop zinc-deficiency are shown as multilist more now：Plant is small, blade
Chlorosis, there is grayish green or yellowish-white spot, and root growth is undeveloped.
3, the boron content of Different Crop is widely different, and dicotyledonous crops are significantly higher than monocot crops, wherein again with pulse family
Crop and crop in cruciferae highest, and gramineous crop is minimum.In crop Different Organs, the resultant of boron element is also different,
The content highest of leaf, flower take second place, and minimum in the organs such as stem, root, fruit, seed.Boron can reinforce the photosynthesis of crop, promote
Into the normal operation of carbohydrate, promote the normal development of crop propagating organ, is conducive to blossom and bear fruit.Apply suitable boron
Fertilizer can accelerate Floral development, increase pollen quantity, promote the sprouting of pollen grain and the growth of pollen tube.Crop boron deficiency top is raw
The abnormal or growth arrest of long point, spire is lopsided, shrinkage, and chlorosis between vein, lower blade thickeies, and leaf color is deepened, and plant is short and small.
Although the boron element in line borate is not the composition of various organic matters in crop body, certain important lifes of crop can be reinforced
Manage function.Major function has：
1. promoting cell elongation and tissue differentiation.
2. accelerating enzyme metabolism and lignin being formed.
3. carbohydrate transport and protein metabolism.
4. promoting root growth and development.
5. enhancing crop anti-adversity.
6. promoting early ripening of the crops modification.
7. promoting pollen germination and pollen tube growth.
4, ferric sulfate：Although iron is not the ingredient of chlorophyll, when ferro element subalimentation, can make the synthesis of chlorophyll by
To obstruction, blade occurs chlorosis phenomenon, influences the formation of photosynthesis and carbohydrate.Since iron in crop body cannot be once again
It utilizes, therefore symptom is since spire, when crop iron deficiency, mainly chlorophyll is destroyed, chlorosis between vein, pear tree iron deficiency, leaf
Piece chlorosis yellow.
5, manganese chloride：Distribution of the manganese in crop organ, usually most with blade, followed by stalk, is seed again, green
Color part is more than non-green part.Manganese is the constituent of chloroplaset, is nutrient necessary to maintain chloroplast structure, can promote
Into the photosynthesis of crop.Cotton applies manganese fertilizer, not only mitigates fruit abscission rate phenomenon, but also it is aobvious to make to harvest level-one unginned cotton earlier
Work increases.Manganese cannot recycle in crop body, and plant manganese deficiency symptom shows spire first：The chlorophyll of blade is reduced, leaf
Chlorosis between arteries and veins, and vein and vein nearby still maintain green.
Fluid nutrient medium, the equivalent of solid medium are the liquid culture mediums of microorganism or animal and plant cells.It has
The advantages of carrying out ventilation culture, shaken cultivation.Under quiescent conditions, it around thallus or culture cell, is formed through feeding
The obstacle divided, the intake of nutrient are hindered.Since this obstruction and increase can be eliminated in ventilation or under conditions of oscillation
Oxygen-supplying amount improves output so being conducive to cell growth.
The present invention also provides a kind of preparation methods of tobacco fertilizer special for organic, include the following steps：
Step 1: being expanded culture respectively to bacillus subtilis and saccharomycete, to bacillus subtilis and saccharomycete
Expand culture that detailed process is as follows：
(1) strain is inoculated in solid slope culture medium, 32~38h of activation culture under the conditions of 35 DEG C, activation is made
Seed；
(2) activated seed made from step (1) is inoculated in fluid nutrient medium, revolving speed be 180~220r/min,
Under conditions of temperature is 35 DEG C, first order seed is made in 32~38h of shaken cultivation；
(3) first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 35h under the conditions of 32~38 DEG C, and secondary seed is made；
(4) secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 48h under the conditions of 32~38 DEG C, and zymocyte liquid is made.
Zymocyte liquid is uniformly mixed according to the proportion after filters pressing, flash evaporation drying, and mixed microorganism yeast powder is made.
It is dried Step 2: being sent into drying tower after corn stover is dried, drying temperature is 50~70 DEG C, is sent after drying
Enter high speed disintegrator to be crushed, be sieved, corn stalk powder is made.
Step 3: by corn stalk powder and peroxophosphoric acid made from mixed microorganism yeast powder made from step 1, step 2
Calcium, Diammonium phosphate (DAP), potassium fulvate, copper molybdate, zinc nitrate, line borate, ferric sulfate, manganese chloride and water are uniformly mixed, and tobacco is made
Fertilizer special for organic.
The preparation of 1 tobacco fertilizer special for organic of embodiment
Step 1: being expanded culture respectively to bacillus subtilis and saccharomycete, to bacillus subtilis and saccharomycete
Expand culture that detailed process is as follows：
(1) strain is inoculated in solid slope culture medium, activation culture 35h under the conditions of 35 DEG C, activated seed is made；
(2) activated seed made from step (1) is inoculated in fluid nutrient medium, revolving speed is 200r/min, temperature is
Under conditions of 35 DEG C, first order seed is made in shaken cultivation 35h；
(3) first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 35h under the conditions of 35 DEG C, and secondary seed is made；
(4) secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 48h under the conditions of 35 DEG C, and zymocyte liquid is made.
It is mixed to weigh bacillus subtilis 1.5kg, saccharomycete 2kg after filters pressing, flash evaporation drying according to the proportion for zymocyte liquid
It closes uniformly, mixed microorganism yeast powder is made.
Step 2: drying drying tower is sent into after the sunning of 58kg corn stover, drying temperature is 60 DEG C, is sent after drying
Enter high speed disintegrator to be crushed, be sieved, corn stalk powder is made.
Step 3: by corn stalk powder and 2.5kg made from mixed microorganism yeast powder made from step 1, step 2
Calcium superphosphate, 1.5kg Diammonium phosphate (DAP), 13kg potassium fulvate, trace element mixture 9kg (copper molybdate, zinc nitrate, line borate, sulphur
Sour iron and manganese chloride), 18kg water be uniformly mixed, be made tobacco fertilizer special for organic.
The preparation of 2 tobacco fertilizer special for organic of embodiment
Step 1: being expanded culture respectively to bacillus subtilis and saccharomycete, to bacillus subtilis and saccharomycete
Expand culture that detailed process is as follows：
(1) strain is inoculated in solid slope culture medium, activation culture 32h under the conditions of 35 DEG C, activated seed is made；
(2) activated seed made from step (1) is inoculated in fluid nutrient medium, revolving speed is 180r/min, temperature is
Under conditions of 35 DEG C, first order seed is made in shaken cultivation 38h；
(3) first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 35h under the conditions of 32 DEG C, and secondary seed is made；
(4) secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 48h under the conditions of 38 DEG C, and zymocyte liquid is made.
Zymocyte liquid weighs bacillus subtilis 1kg, saccharomycete 3kg mixing after filters pressing, flash evaporation drying according to the proportion
Uniformly, mixed microorganism yeast powder is made.
Step 2: drying drying tower is sent into after the sunning of 60kg corn stover, drying temperature is 50 DEG C, is sent after drying
Enter high speed disintegrator to be crushed, be sieved, corn stalk powder is made.
Step 3: by corn stalk powder made from mixed microorganism yeast powder made from step 1, step 2 and 2kg mistake
Calcium phosphate, 1kg Diammonium phosphate (DAP), 15kg potassium fulvate, trace element mixture 8kg (copper molybdate, zinc nitrate, line borate, ferric sulfate
And manganese chloride), 10kg water be uniformly mixed, be made tobacco fertilizer special for organic.
The preparation of 3 tobacco fertilizer special for organic of embodiment
Step 1: being expanded culture respectively to bacillus subtilis and saccharomycete, to bacillus subtilis and saccharomycete
Expand culture that detailed process is as follows：
(1) strain is inoculated in solid slope culture medium, activation culture 38h under the conditions of 35 DEG C, activated seed is made；
(2) activated seed made from step (1) is inoculated in fluid nutrient medium, revolving speed is 220r/min, temperature is
Under conditions of 35 DEG C, first order seed is made in shaken cultivation 32h；
(3) first order seed made from step (2) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 35h under the conditions of 38 DEG C, and secondary seed is made；
(4) secondary seed made from step (3) is inoculated in fluid nutrient medium by 9% (percent by volume), oxygen dissolved
45% or more, tank presses 0.45Mpa, seed tank culture 48h under the conditions of 32 DEG C, and zymocyte liquid is made.
It is mixed to weigh bacillus subtilis 2kg, saccharomycete 1.5kg after filters pressing, flash evaporation drying according to the proportion for zymocyte liquid
It closes uniformly, mixed microorganism yeast powder is made.
Step 2: drying drying tower is sent into after the sunning of 55kg corn stover, drying temperature is 70 DEG C, is sent after drying
Enter high speed disintegrator to be crushed, be sieved, corn stalk powder is made.
Step 3: by corn stalk powder made from mixed microorganism yeast powder made from step 1, step 2 and 3kg mistake
Calcium phosphate, 2kg Diammonium phosphate (DAP), 10kg potassium fulvate, trace element mixture 10kg (copper molybdate, zinc nitrate, line borate, sulfuric acid
Iron and manganese chloride), 15kg water be uniformly mixed, be made tobacco fertilizer special for organic.
Compliance test result test
As a comparison using tobacco fertilizer special for organic obtained in embodiment 1-3 and using commercially available chemical fertilizer, it applies
It is as follows with method：It is applied to before transplanting in tobacco ground, after transplanting using fertilizer lid, in growth period again to tobacco top dressing.
Influence of the different fertilizer to cigarette quality and yield is as shown in the table.
| Yield (kg/666.7m2) | Quality | |
| Embodiment 1 | 158.3 | Blade is big, takes root that thick, color is good |
| Embodiment 2 | 157.1 | Blade is big, takes root that thick, color is good |
| Embodiment 3 | 156.5 | Blade is big, takes root that thick, color is good |
| Commercially available chemical fertilizer | 130.5 | Blade is moderate, it is general to take root, color is shallower |
According to test result it is found that apply tobacco fertilizer special for organic of the invention can significantly improve tobacco yield and
Quality, yield are up to 158.3kg/666.7m2, compare existing commercially available chemical fertilizer, output increased 16.61~17.37%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of tobacco fertilizer special for organic, which is characterized in that according to parts by weight include following component：
55~60 parts of corn stover；2~3 parts of calcium superphosphate；1~2 part of Diammonium phosphate (DAP)；10~15 parts of potassium fulvate；Withered grass gemma
1~2 part of bacillus；1.5~3 parts of saccharomycete；8~10 parts of trace element mixture；15~20 parts of water；
The trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride, copper molybdate, zinc nitrate,
Weight ratio between line borate, ferric sulfate and manganese chloride is 1:1.3:1.5:2:1.5.
2. a kind of tobacco fertilizer special for organic according to claim 1, which is characterized in that according to parts by weight include with the following group
Part：
58 parts of corn stover；2.5 parts of calcium superphosphate；1.5 parts of Diammonium phosphate (DAP)；13 parts of potassium fulvate；1.5 parts of bacillus subtilis；
2 parts of saccharomycete；9 parts of trace element mixture；18 parts of water；
The trace element mixture includes copper molybdate, zinc nitrate, line borate, ferric sulfate and manganese chloride.
3. a kind of tobacco fertilizer special for organic according to claim 1, which is characterized in that the bacillus subtilis and yeast
Effective bacterium number in bacterium is 8~1,000,000,000/mL.
4. the method for preparing a kind of tobacco fertilizer special for organic described in any one of claims 1 to 3, which is characterized in that packet
Include following steps：
Step 1: being expanded culture respectively to bacillus subtilis and saccharomycete, after filters pressing, flash evaporation drying, according to the proportion
It is uniformly mixed, mixed microorganism yeast powder is made；
Step 2: drying after corn stover is dried, drying temperature is 50~70 DEG C, is crushed, is sieved after drying, is made
Obtain corn stalk powder；
Step 3: by corn stalk powder made from mixed microorganism yeast powder made from step 1, step 2 and calcium superphosphate, phosphorus
Sour diammonium, potassium fulvate, copper molybdate, zinc nitrate, line borate, ferric sulfate, manganese chloride and water are uniformly mixed, and are made that tobacco is dedicated to be had
Machine fertilizer.
5. the preparation method according to claim 4, which is characterized in that in step 2, drying temperature is 60 DEG C.
6. the preparation method according to claim 4, which is characterized in that in step 1, to bacillus subtilis and saccharomycete
Expand culture that detailed process is as follows：
Step 1: strain is inoculated in solid slope culture medium, 32~38h of activation culture under the conditions of 35 DEG C, activation is made
Seed；
It is 180~220r/min, temperature in revolving speed Step 2: activated seed made from step 1 is inoculated in fluid nutrient medium
Under conditions of degree is 35 DEG C, first order seed is made in 32~38h of shaken cultivation；
Step 3: first order seed made from step 2 is inoculated in fluid nutrient medium in the ratio that percent by volume is 9%, it is molten
45% or more oxygen, tank press 0.45Mpa, seed tank culture 35h under the conditions of 32~38 DEG C, and secondary seed is made；
Step 4: secondary seed made from step 3 is inoculated in fluid nutrient medium in the ratio that percent by volume is 9%, it is molten
45% or more oxygen, tank press 0.45Mpa, seed tank culture 48h under the conditions of 32~38 DEG C, and zymocyte liquid is made.
7. preparation method according to claim 6, which is characterized in that the component and dosage of the solid slope culture medium are such as
Under：1L distilled water；20g glucose；15g peptone；5g sodium chloride；0.5g beef extract；20g agar.
8. preparation method according to claim 6, which is characterized in that the component and dosage of the fluid nutrient medium are as follows：
Casein peptone 17g；Soy peptone 3g；Sodium chloride 5g；Dipotassium hydrogen phosphate 2.5g；Glucose 2.5g；Lecithin 1g；Tween
807g；The pH of the fluid nutrient medium is 7.2 ± 0.2.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810847699.3A CN108863663A (en) | 2018-07-27 | 2018-07-27 | A kind of tobacco fertilizer special for organic and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810847699.3A CN108863663A (en) | 2018-07-27 | 2018-07-27 | A kind of tobacco fertilizer special for organic and preparation method thereof |
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Citations (3)
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|---|---|---|---|---|
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| CN107778095A (en) * | 2017-12-11 | 2018-03-09 | 吉林省利泽生物科技有限公司 | A kind of environment-friendly type maize straw fertilizer and preparation method thereof |
| CN107815317A (en) * | 2017-11-08 | 2018-03-20 | 长春枫晟农牧科技发展有限公司 | A kind of microbe soil conditioner and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614452A (en) * | 2013-11-13 | 2014-03-05 | 烟台市喜旺食品有限公司 | Composite culture medium and method for quickly detecting total bacterial count by using same |
| CN107815317A (en) * | 2017-11-08 | 2018-03-20 | 长春枫晟农牧科技发展有限公司 | A kind of microbe soil conditioner and preparation method thereof |
| CN107778095A (en) * | 2017-12-11 | 2018-03-09 | 吉林省利泽生物科技有限公司 | A kind of environment-friendly type maize straw fertilizer and preparation method thereof |
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| 文天雁: "《有机肥与中微量元素配施对烤烟产量及品质的影响》", 《山地农业生物学报》 * |
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Application publication date: 20181123 |