CN109761671A - A kind of liquid fertilizer and preparation method thereof promoting winter jujube flower bud development - Google Patents
A kind of liquid fertilizer and preparation method thereof promoting winter jujube flower bud development Download PDFInfo
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- CN109761671A CN109761671A CN201711101421.3A CN201711101421A CN109761671A CN 109761671 A CN109761671 A CN 109761671A CN 201711101421 A CN201711101421 A CN 201711101421A CN 109761671 A CN109761671 A CN 109761671A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a kind of liquid fertilizers and preparation method thereof for promoting winter jujube flower bud development.The liquid fertilizer includes 1000 parts of active liquid fertilizer substrate preparation；10 ~ 20 parts of nitrogen, 5 ~ 10 parts of potassium, 0.5 ~ 5 part of phosphorus, 1 ~ 6 part of calcium, 1 ~ 6 part of magnesium, 2 ~ 10 parts of boron, 0.1 ~ 3 part of zinc, 0.1 ~ 3 part of molybdenum, 0.1 ~ 3 part of iron；Contain 11 ~ 20 parts of proline, 12 ~ 22 parts of glycine, 15 ~ 23 parts of leucine, 6 ~ 15 parts of lysine, 10 ~ 18 parts of tryptophan, 2 ~ 10 parts of alanine, 1 ~ 10 part of methionine, 5 ~ 13 parts of tyrosine in the liquid fertilizer；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/L；Liquid fertilizer of the invention has selectivity to the bud differentiation of different jujube trees, the most significant to the bud differentiation effect of winter jujube.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid for promoting winter jujube flower bud development
Fertilizer and preparation method thereof.
Background technique
Winter jujube is a late-maturing fresh food excellent variety for stingless jujube tree, and the Zizyphus jujuba Mill kind that the quality generally acknowledged is best.
Winter-jujube fruit also contains the various trace elements such as more VitAVitE, potassium, sodium, iron, copper, there is holding capillary
It is unimpeded, prevent the increased function of vascular wall brittleness, it is effective in cure for hypertension, Atheromatosis disease, have the function of anti-cancer
Effect.Nutritive value is the hat of all kinds of fruits, there is the title of " all kinds of fruits king ".Big in fruit, subcircular exactly likes griggles, Mean Fruit Weight 14.5
Gram, maximum fruit weighs 46 grams, and uniform in size neat, fruit face is smooth, pericarp crisp fritter, and the white ripe phase is in light green color, after transmitted by light brown
Ember.Pulp matter is thin, and tender and crisp succulence, dense sweet tea is slightly sour, very palatable, soluble solid content crisp ripe phase be 34%~
38%, up to 40%~42% before complete ripeness, quality extremely on.Fruit stone oval, average to weigh 0.6 gram or so, core line is shallow, taeniae
Line, kernel-containing rate is high, up to 100%.
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Chain
Mould is widely present in soil, is Filamentous gram-positive bacterium, mainly passes through sporogenesis, most streptomycetes
It is harmless.Streptomycete is the main source for generating various antibiotic, and the antibiotic derived from streptomycete includes erythromycin, four
Ring element, streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for chain
The existing research of mould focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
Bud differentiation refers to that axis growing point is changed into the mistake for differentiating inflorescence or flower by mitogenetic blade, axillary bud out
Journey, bud differentiation are the physiology and morphological landmarks changed from nutrient growth to reproductive growth.Bud differentiation directly influences plant
Yield positive results, therefore flower bud differentiation period has directly established the basis of fruiting period and harvest time to the nutritional supplementation of plant.
Summary of the invention
The problem of the technical problem to be solved by the present invention is to overcome existing winter jujube root growths provides a kind of promotion winter jujube
Liquid fertilizer of flower bud development and preparation method thereof.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer promoting winter jujube flower bud development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；10~20 parts of nitrogen, 5~10 parts of potassium, 0.5~5 part of phosphorus, 1~6 part of calcium, magnesium
1~6 part, 2~10 parts of boron, 0.1~3 part of zinc, 0.1~3 part of molybdenum, 0.1~3 part of iron；
Contain 11~20 parts of proline, 12~22 parts of glycine, 15~23 parts of leucine, bad ammonia in the liquid fertilizer
Sour 6~15 parts, 10~18 parts of tryptophan, 2~10 parts of alanine, 1~10 part of methionine, 5~13 parts of tyrosine；Total nitrogen content
>=2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/L；The active liquid fertilizer substrate preparation is by chain
Mould category bacterial strain " TY-001# " fermentation obtains；
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation,
It is beaten, filtered；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is
7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is Guangdong province, China
Xianlie Middle Road, Guangzhou City 100 five building, Institute of Micro-biology, province laboratory building.
Further, the liquid fertilizer for promoting winter jujube flower bud development, by the material composition of following parts by weight: activity
1000 parts of Liquid Fertilizer substrate preparation；13~18 parts of nitrogen, 6~8 parts of potassium, 1~3 part of phosphorus, 2~5 parts of calcium, 2~4 parts of magnesium, boron 5~8
Part, 0.8~1.2 part of zinc, 0.8~1.5 part of molybdenum, 0.6~1.3 part of iron；
Contain 14~16 parts of proline, 15~19 parts of glycine, 17~20 parts of leucine, bad ammonia in the liquid fertilizer
Sour 8~11 parts, 13~16 parts of tryptophan, 4~7 parts of alanine, 2~8 parts of methionine, 7~11 parts of tyrosine；Total nitrogen content >=
2.8mg/g, amino nitrogen content >=0.6mg/g, the content of organic matter >=120g/L.
Preferably, the liquid fertilizer for promoting winter jujube flower bud development, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation；15 parts of nitrogen, 7.5 parts of potassium, 1.5 parts of phosphorus, 3 parts of calcium, 3 parts of magnesium, 6 parts of boron, zinc
1 part, 1 part of molybdenum, 0.8 part of iron；
Contain 15 parts of proline, 16 parts of glycine, 18 parts of leucine, 10 parts of lysine, tryptophan in the liquid fertilizer
14 parts, 6 parts of alanine, 6 parts of methionine, 8 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, has
Machine matter content >=120g/L.
Preferably, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Further, the nitrogen, phosphorus, potassium ratio be 10:1:5.
Further, the zinc, molybdenum, iron ratio be 5:5:4.
A kind of preparation method for the liquid fertilizer promoting winter jujube flower bud development, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then
After sugar is added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C
After it, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. the configuration of liquid fertilizer: toward the active liquid fertilizer substrate preparation addition proline, glycine, leucine,
One of methionine, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron are several
Kind, obtain the liquid fertilizer；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is Guangdong province, China
Xianlie Middle Road, Guangzhou City 100 five building, Institute of Micro-biology, province laboratory building.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.1g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
S1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~
7 days, bacterial strain after activation is made；
S2. strain inoculated is 25~30 DEG C, revolution in temperature into fluid nutrient medium after taking step S1 activation obtained
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；
White granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer of the invention is capable of the bud differentiation of conspicuousness raising winter jujube, can quickly supplement winter jujube growth institute
The nutrient needed promotes flowers and fruits growth, is conducive to the growth and development of plant.Apply the winter jujube plant of liquid fertilizer of the invention
New-tip length increases 15.8%, and young sprout rugosity increases 9.5%.In terms of bud rate, bud of the liquid fertilizer to winter jujube
Rate promotion increases 11.8%, and flower number increases 13.1%, achieves unexpected technical effect, and tree body leaf color is dark green,
Chlorophyll content increases obvious.Liquid fertilizer of the invention has very significant effect to the bud differentiation of winter jujube.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but embodiment does not do any form to the present invention
Restriction.Unless stated otherwise, the present invention uses reagent, method and apparatus for the art conventional reagent, method and are set
It is standby.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to
1 liter, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15~
25g, water are settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C are transferred to
It is saved in refrigerator.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, 220 revs/min on oscillation shaking table, 25 DEG C of conditions
Lower culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow
Best surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid culture
Sodium chloride dosage in base and fluid nutrient medium all increases by 1 gram, until the sodium chloride in solid medium and fluid nutrient medium is used
Amount reaches 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore gram dye
Color is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
The embodiment 2 a kind of preparation and application of the liquid fertilizer for promoting winter jujube flower bud development
It is a kind of promote winter jujube flower bud development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, it will
Filtrate is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration sugared in culture medium
100g/L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain
To liquid fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
The liquid fertilizer of winter jujube flower bud development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；15 parts of nitrogen, 7.5 parts of potassium, 1.5 parts of phosphorus, 3 parts of calcium, 3 parts of magnesium, 6 parts of boron, zinc
1 part, 1 part of molybdenum, 0.8 part of iron；
Contain 15 parts of proline, 16 parts of glycine, 18 parts of leucine, 10 parts of lysine, tryptophan in the liquid fertilizer
14 parts, 6 parts of alanine, 6 parts of methionine, 8 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, has
Machine matter content >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe be respectively derived from ammonium dihydrogen phosphate and amino acid and
Ammonium molybdate, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The embodiment 3 a kind of preparation and application of the liquid fertilizer for promoting winter jujube flower bud development
It is a kind of promote winter jujube flower bud development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g dregs of beans to carry out pre fermentation, being put into fiberizer and be beaten, filter, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration sugared in culture medium
100g/L, pH 7.5；
Pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 4 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, inside fermentation raw material
When temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days.
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain
To liquid fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
The liquid fertilizer of winter jujube flower bud development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；18 parts of nitrogen, 9 parts of potassium, 1.8 parts of phosphorus, 5 parts of calcium, 4 parts of magnesium, 8 parts of boron, zinc
1.5 parts, 1.5 parts of molybdenum, 1.2 parts of iron；Contain 16 parts of proline in the liquid fertilizer, 19 parts of glycine, 20 parts of leucine, rely
11 parts of propylhomoserin, 16 parts of tryptophan, 7 parts of alanine, 8 parts of methionine, 11 parts of tyrosine；Total nitrogen content >=2.8mg/g, ammonia nitrogen
Content >=0.6mg/g, the content of organic matter >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe are respectively derived from di(2-ethylhexyl)phosphate
Hydrogen ammonium and amino acid and ammonium molybdate, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, molybdic acid
Ammonium, ferric sulfate.
The embodiment 4 a kind of preparation and application of the liquid fertilizer for promoting winter jujube flower bud development
It is a kind of promote winter jujube flower bud development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, it will
Filtrate is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration sugared in culture medium
100g/L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain
To liquid fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
The liquid fertilizer of winter jujube flower bud development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；10 parts of nitrogen, 5 parts of potassium, 1 part of phosphorus, 1 part of calcium, 1 part of magnesium, 2 parts of boron, 1 part of zinc,
1 part of molybdenum, 0.8 part of iron；
Contain 11 parts of proline, 12 parts of glycine, 15 parts of leucine, 6 parts of lysine, tryptophan in the liquid fertilizer
10 parts, 2 parts of alanine, 1 part of methionine, 5 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, has
Machine matter content >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe be respectively derived from ammonium dihydrogen phosphate and amino acid and
Ammonium molybdate, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The embodiment 5 a kind of preparation and application of the liquid fertilizer for promoting winter jujube flower bud development
It is a kind of promote winter jujube flower bud development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, it will
Filtrate is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration sugared in culture medium
100g/L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain
To liquid fertilizer；
Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
The liquid fertilizer of winter jujube flower bud development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；20 parts of nitrogen, 10 parts of potassium, 2 parts of phosphorus, 6 parts of calcium, 6 parts of magnesium, 10 parts of boron, zinc 3
Part, 3 parts of molybdenum, 2.4 parts of iron；
Contain 20 parts of proline, 22 parts of glycine, 23 parts of leucine, 15 parts of lysine, tryptophan in the liquid fertilizer
18 parts, 10 parts of alanine, 10 parts of methionine, 13 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g,
The content of organic matter >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe be respectively derived from ammonium dihydrogen phosphate and amino acid with
And ammonium molybdate, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
The comparative example 1 a kind of preparation and application of the liquid fertilizer for promoting winter jujube flower bud development
It is a kind of promote winter jujube flower bud development liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, it will
Filtrate is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration sugared in culture medium
100g/L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation.
S4. the configuration of liquid fertilizer: toward the active liquid fertilizer substrate preparation addition proline, glycine, leucine,
One of methionine, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron are several
Kind, obtain the liquid fertilizer；
The liquid fertilizer of winter jujube flower bud development by following parts by weight material composition:
1000 parts of active liquid fertilizer substrate preparation；10 parts of nitrogen, 10 parts of potassium, 5 parts of phosphorus, 1 part of calcium, 1 part of magnesium, 2 parts of boron, zinc 1
Part, 1 part of molybdenum, 1 part of iron；
Contain 11 parts of proline, 12 parts of glycine, 15 parts of leucine, 6 parts of lysine, tryptophan in the liquid fertilizer
10 parts, 2 parts of alanine, 1 part of methionine, 5 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, has
Machine matter content >=120g/L.Wherein N, P, K, Ca, B, Mg, Zn, Mo, Fe be respectively derived from ammonium dihydrogen phosphate and amino acid and
Ammonium molybdate, ammonium dihydrogen phosphate, potassium sulfate, calcium gluconate, boric acid, magnesium sulfate, zinc chloride, ammonium molybdate, ferric sulfate.
Promote the application effect appraisal of the liquid fertilizer of winter jujube flower bud development:
Nutrient needed for liquid fertilizer can quickly supplement fruit tree growth promotes flowers and fruits growth.It is prepared by the present invention to verify
The liquid fertilizer actual effect that large-scale demonstration is applied on fruit tree, applicant's selection have carried out experiment and demonstration in winter jujube.
1 materials and methods
1.1 are located in Zixing City winter jujube base for examination place 1, and 12 mu of testing ground area, soil fertility level is more equal
It is even.
It is located in Jianning city variety of the Chinese date base for examination place 2,12 mu of testing ground area, soil fertility level is more uniform.
1.2 for studying 1 winter jujube of object, First Year after transplanting, seeding row spacing 4m*4m, the growing way golden mean of the Confucian school and consistent.
For studying 2 variety of the Chinese date of object, First Year after transplanting, seeding row spacing 4m*4m, the growing way golden mean of the Confucian school and consistent.
1.3 for examination fertilizer embodiment 2 made from winter jujube flower bud development liquid fertilizer, product forms are liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group.It pours within 1 month before florescence and applies primary, 500mL/ mus each, 200 times of dilution；Full-bloom stage sprays 1 time, often
It is 500mL/ mus secondary, dilute 200 times.
2. control group (CK).Per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: it is carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other
The same processing of measures of fertilizer is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.10 plants of jujube tree measurement bud differentiation situations are randomly selected, average value is acquired.
2 results and analysis
2.1 different disposals are to young sprout growth, the influence of bud rate and flower number
As shown in Table 1, after applying the liquid fertilizer, the new-tip length and rugosity of winter jujube are significantly higher than control group and right
Ratio group, length increases 15.8% compared to the control group, and rugosity increases 9.5%.In terms of bud rate, the liquid fertilizer pair
The bud rate promotion of winter jujube increases 11.8%, and flower number increases 13.1%, achieves unexpected technical effect.So
And the liquid fertilizer does not all play substantive effect to the growth of flower bud of the variety of the Chinese date.As it can be seen that liquid fertilizer pair of the invention
The bud differentiation of different jujube trees has selectivity, the most significant to the bud differentiation effect of winter jujube.
Young sprout growth, bud rate and the flower number situation result of the different winter jujube tree quality different disposals of table 1
| Processing | New-tip length, cm | Young sprout rugosity, cm | Bud rate % | Flower number/piece |
| Winter jujube experimental group | 82.7 | 0.81 | 90.1 | 2095 |
| Winter jujube control group | 71.4 | 0.74 | 80.6 | 1852 |
| Winter jujube comparative example group | 75.7 | 0.76 | 85.4 | 1901 |
| Variety of the Chinese date experimental group | 72.8 | 0.62 | 73.2 | 1796 |
| Variety of the Chinese date control group | 72.4 | 0.57 | 66.7 | 1713 |
| Variety of the Chinese date comparative example group | 72.5 | 0.60 | 71.0 | 1753 |
Claims (10)
1. a kind of liquid fertilizer for the liquid fertilizer winter jujube flower bud development for promoting winter jujube flower bud development, which is characterized in that by following
The material composition of parts by weight:
1000 parts of active liquid fertilizer substrate preparation；10 ~ 20 parts of nitrogen, 5 ~ 10 parts of potassium, 0.5 ~ 5 part of phosphorus, 1 ~ 6 part of calcium, 1 ~ 6 part of magnesium, boron 2
~ 10 parts, 0.1 ~ 3 part of zinc, 0.1 ~ 3 part of molybdenum, 0.1 ~ 3 part of iron；
In the liquid fertilizer containing 11 ~ 20 parts of proline, 12 ~ 22 parts of glycine, 15 ~ 23 parts of leucine, 6 ~ 15 parts of lysine,
10 ~ 18 parts of tryptophan, 2 ~ 10 parts of alanine, 1 ~ 10 part of methionine, 5 ~ 13 parts of tyrosine；Total nitrogen content >=2.8mg/g, ammonia nitrogen
Content >=0.6mg/g, the content of organic matter >=120g/L；The active liquid fertilizer substrate preparation is by streptomyces bacterial strain " TY-
001# " fermentation obtains；
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, beaten
Slurry, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. promoting the liquid fertilizer of winter jujube flower bud development according to claim 1, which is characterized in that by the object of following parts by weight
Matter composition:
1000 parts of active liquid fertilizer substrate preparation；13 ~ 18 parts of nitrogen, 6 ~ 8 parts of potassium, 1 ~ 3 part of phosphorus, 2 ~ 5 parts of calcium, 2 ~ 4 parts of magnesium, boron 5 ~ 8
Part, 0.8 ~ 1.2 part of zinc, 0.8 ~ 1.5 part of molybdenum, 0.6 ~ 1.3 part of iron；
In the liquid fertilizer containing 14 ~ 16 parts of proline, 15 ~ 19 parts of glycine, 17 ~ 20 parts of leucine, 8 ~ 11 parts of lysine,
13 ~ 16 parts of tryptophan, 4 ~ 7 parts of alanine, 2 ~ 8 parts of methionine, 7 ~ 11 parts of tyrosine；Total nitrogen content >=2.8mg/g, ammonia nitrogen contain
Amount >=0.6mg/g, the content of organic matter >=120g/L.
3. promoting the liquid fertilizer of winter jujube flower bud development according to claim 1, which is characterized in that by the object of following parts by weight
Matter composition:
1000 parts of active liquid fertilizer substrate preparation；15 parts of nitrogen, 7.5 parts of potassium, 1.5 parts of phosphorus, 3 parts of calcium, 3 parts of magnesium, 6 parts of boron, 1 part of zinc,
1 part of molybdenum, 0.8 part of iron；
In the liquid fertilizer containing 15 parts of proline, 16 parts of glycine, 18 parts of leucine, 10 parts of lysine, 14 parts of tryptophan,
6 parts of alanine, 6 parts of methionine, 8 parts of tyrosine；Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.6mg/g, organic matter contain
Amount >=120g/L.
4. promoting the liquid fertilizer of winter jujube flower bud development according to claim 1, which is characterized in that the pre-fermented step
Are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans clenching of the hand reaches the state for dripping into line；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3 ~ 5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material
At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2 ~ 3 days.
5. according to claim 1 promote winter jujube flower bud development liquid fertilizer, which is characterized in that the nitrogen, phosphorus, potassium ratio
Example is 10:1:5.
6. according to claim 1 promote winter jujube flower bud development liquid fertilizer, which is characterized in that the zinc, molybdenum, iron ratio
Example is 5:5:4.
7. a kind of preparation method of the liquid fertilizer for promoting winter jujube flower bud development, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. proline, glycine, leucine, egg ammonia the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
One or more of acid, tyrosine, tryptophan, alanine, lysine or nitrogen, phosphorus, potassium, calcium, magnesium, boron, zinc, molybdenum, iron, obtain
The liquid fertilizer；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017,
Classification naming is Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China
City martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
8. promoting the preparation method of the liquid fertilizer of winter jujube flower bud development according to claim 7, which is characterized in that the work
Property Liquid Fertilizer substrate preparation store 15 days after its amino-acid nitrogen content >=4.1g/L.
9. promoting the preparation method of the liquid fertilizer of winter jujube flower bud development according to claim 7, which is characterized in that the step
In rapid S1 plus water is settled to concentration sugared in culture medium as 100g/L.
10. any one of claim 1 to 9 liquid fertilizer for promoting winter jujube flower bud development cultivates Process of Flower Bud Differentiation in winter jujube
In application.
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