CN109956778A - A kind of liquid fertilizer resistant to lodging and preparation method thereof - Google Patents
A kind of liquid fertilizer resistant to lodging and preparation method thereof Download PDFInfo
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- CN109956778A CN109956778A CN201711427042.3A CN201711427042A CN109956778A CN 109956778 A CN109956778 A CN 109956778A CN 201711427042 A CN201711427042 A CN 201711427042A CN 109956778 A CN109956778 A CN 109956778A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of liquid fertilizer and preparation method thereof resistant to lodging, which includes 1000 parts of active liquid fertilizer substrate preparation, multiple 0.1 ~ 5 part of phthalein nucleic acid, 12 ~ 28 parts of Water-soluble silicon fertilizer, 52 ~ 70 parts of urea, amino acid 1 ~ 10 part, 52 ~ 72 parts of potassium sulfate, 10 ~ 25 parts of calcium superphosphate, 1 ~ 10 part of boric acid, 0.1 ~ 5 part of white vitriol；Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are 110 ~ 145g/L.The liquid fertilizer can significantly improve corn rate resistant to lodging, prevent corn lodging, improve the per mu yield of corn.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid fertilizer and its system resistant to lodging
Preparation Method.
Background technique
Fruit corn is a kind of super-sweet corn for being suitble to eat it raw, and green stick stage thin skin, juice is more, matter is crisp and sweet tea, can directly be given birth to
Eat, for very thin epidermis once stinging brokenly, the juice of faint scent overflows tooth cheek, eat it raw it is ripe eat all special sweet tea, especially crisp, as fruit,
Therefore it is referred to as " fruit corn ".Fruit cornBelong to one of corn, is the developed countries such as American-European, South Korea and Japan
One of main vegetables, because that it is with nutrition abundant, sweet tea, fresh, crisp, tender characteristic is deep by each stratum consumers.Production
In corn can be divided into conventional corn, super-sweet corn and reinforced corn three classes, super-sweet corn is high, suitable due to sugar content
Suitable picking time is long and is planted extensively.Corn also has cultivation in China.Plant is shorter, and tillering ability is stronger.On fruit ear bract
HaveBoot leaf.Seed is yellowish or white, and embryo is larger, and milk stage is tender in fact, is rich in water-soluble polysaccharide, vitamin A, vitamin C, rouge
Fat and protein etc..
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
Often there is phenomena such as lodging, inane fruit in fruit corn mesoderm growing early stage, leads to low fruit yields, perishable, mainly
Since deficiency of applying fertilizer in cultivation causes each allelotaxis of plant bad, generation is resistant to lodging, has seriously affected the yield of fruit,
Limit the growing and cultivation of fruit corn.
Summary of the invention
The problem of the technical problem to be solved by the present invention is to overcome the lodging of existing fruit corn, low output provides a kind of anti-
Liquid fertilizer of lodging and preparation method thereof.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer resistant to lodging, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation, multiple 0.1~5 part of phthalein nucleic acid, 12~28 parts of Water-soluble silicon fertilizer, urea 52~
70 parts, amino acid 1~10 part, 52~72 parts of potassium sulfate, 10~25 parts of calcium superphosphate, 1~10 part of boric acid, white vitriol 0.1
~5 parts, total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter is 110~
145g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain
The culture medium of " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Nitrogen exists in the form of amino acid in liquid fertilizer in the present invention is easier to plant absorption utilization, Water-soluble silicon fertilizer
It can quickly be absorbed by plant, effectively prevent plant resistant to lodging, while the high-absorbility of plant pair ammino acid liquid fertilizer, effectively prevent more
Pollution of the remaining fertilizer to environment.
Preferably, the liquid fertilizer is by following material composition:
1000 parts of active liquid fertilizer substrate preparation, multiple 1~3 part of phthalein nucleic acid, 15~22 parts of Water-soluble silicon fertilizer, urea 59~66
Part, 3~8 parts of amino acid, 58~68 parts of potassium sulfate, 13~21 parts of calcium superphosphate, 3~8 parts of boric acid, 1~3 part of white vitriol；
Total nitrogen content >=2.5mg/g in the liquid fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter are 120~135g/L.
Preferably, the liquid fertilizer is by following material composition:
1000 parts of active liquid fertilizer substrate preparation, multiple 2 parts of phthalein nucleic acid, 19 parts of Water-soluble silicon fertilizer, 64 parts of urea, amino acid 6
Part, potassium sulfate 64,18 parts of calcium superphosphate, 6 parts of boric acid, 2 parts of white vitriol, total nitrogen content >=2.5mg/ in the liquid fertilizer
G, amino nitrogen content >=0.4mg/g, the content of organic matter are 126~131g/L.
Further, the weight ratio of N, P, K are 4~8:1:4~8, preferably 6:1:6 in the liquid fertilizer.
Further, the amino acid is proline, in lysine, tryptophan, tyrosine, phenylalanine, aspartic acid
One or more.Further, the Water-soluble silicon fertilizer is sodium metasilicate, potassium silicate, crosses in sodium disilicate and sodium metasilicate
It is a kind of.
A kind of preparation method of the liquid fertilizer resistant to lodging, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. multiple phthalein nucleic acid, Water-soluble silicon fertilizer, urine the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Element, amino acid, potassium sulfate, calcium superphosphate, boric acid, white vitriol obtain the liquid fertilizer.
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.5g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Liquid fertilizer of the invention can effectively prevent fruit resistant to lodging, significantly improve the fruit quality of fruit corn, fastly
Nutrient needed for speed supplement fruit corn growth, promotes flowers and fruits growth, is conducive to the growth and development of plant.Apply liquid of the invention
The corn seedling of body fertilizer is without lodging phenomenon, and mature plant lodging rate is 0.06%, compared to the control group 9.3%, anti-fall
Volt effect is significant.Nitrogen exists in the form of amino acid in liquid fertilizer in the present invention is easier to plant absorption utilization, water-soluble
Siliceous fertilizer can quickly be absorbed by plant, effectively prevent plant resistant to lodging, while the high-absorbility of plant pair ammino acid liquid fertilizer, effectively anti-
Only pollution of the extra fertilizer to environment.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
The embodiment 2 a kind of preparation and application of liquid fertilizer resistant to lodging
A kind of preparation method of liquid fertilizer resistant to lodging the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 0.1 part of phthalein nucleic acid, silicic acid the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
12 parts of sodium, 52 parts of urea, 1 part of lysine, potassium sulfate 52,10 parts of calcium superphosphate, 1 part of boric acid, 0.1 part of white vitriol obtain liquid
Body fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 110g/
L。
The embodiment 3 a kind of preparation and application of liquid fertilizer resistant to lodging
A kind of preparation method of liquid fertilizer resistant to lodging the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 1 part of phthalein nucleic acid, water solubility the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
15 parts of siliceous fertilizer, 59 parts of urea, 1 part of lysine, 1 part of tryptophan, 1 part of aspartic acid, 58 parts of potassium sulfate, 13 parts of calcium superphosphate, boron
3 parts, 1 part of white vitriol of acid obtains liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 121g/L.
The embodiment 4 a kind of preparation and application of liquid fertilizer resistant to lodging
A kind of preparation method of liquid fertilizer resistant to lodging the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 2 parts of phthalein nucleic acid, water solubility the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
19 parts of siliceous fertilizer, 64 parts of urea, 3 parts of lysine, 3 parts of tryptophan, potassium sulfate 64,18 parts of calcium superphosphate, 6 parts of boric acid, seven water sulfuric acid
2 parts of zinc obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, organic matter
Content is 129g/L.
The embodiment 5 a kind of preparation and application of liquid fertilizer resistant to lodging
A kind of preparation method of liquid fertilizer resistant to lodging the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 5 parts of phthalein nucleic acid, water solubility the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
28 parts of siliceous fertilizer, 70 parts of urea, 4 parts of lysine, 4 parts of tryptophan, 2 parts of aspartic acid, potassium sulfate 72,25 parts of calcium superphosphate, boric acid
10 parts, 5 parts of white vitriol obtain liquid fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=
0.4mg/g, content of organic matter 143g/L.
The comparative example 1 a kind of preparation and application of liquid fertilizer resistant to lodging
A kind of preparation method of liquid fertilizer resistant to lodging the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S4. multiple 2 parts of phthalein nucleic acid, water solubility the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
19 parts of siliceous fertilizer, 64 parts of urea, 6 parts of proline, potassium sulfate 64,41 parts of calcium superphosphate, 6 parts of boric acid, 2 parts of white vitriol obtain liquid
Body fertilizer；Total nitrogen content >=2.5mg/g in gained liquid fertilizer, amino nitrogen content >=0.4mg/g, content of organic matter 135g/
L。
The application effect appraisal of liquid fertilizer resistant to lodging:
Nutrient needed for Amino acid liquid fertilizer can quickly supplement fruit plant strain growth effectively prevent resistant to lodging, promotion fruit
Corn growth improves fruit corn fruit mouthfeel and quality.It is big on fruit corn to verify liquid fertilizer prepared by the present invention
The actual effect of area Demonstration Application, applicant's selection have carried out experiment and demonstration on fruit corn.
1 materials and methods
1.1 for examination place 1 be located in Zixing City fruit corn base, 12 mu of testing ground area, soil fertility level compared with
Uniformly.
1.2 for studying 1 fruit corn of object, plant line-spacing 0.8m*0.4m, the growing way golden mean of the Confucian school and consistent.
1.3 for liquid fertilizer resistant to lodging made from examination fertilizer embodiment 2~5, and product forms are liquid.
1.4 it is each for examination place carry out 3 test process:
1. experimental group: every month, which pours, after plantation applies once for trying fertilizer, 500mL/ mus each, dilutes 200 times.
2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing
①。
3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
It for cell, is repeated 3 times, management is consistent.100 square metres of fruit corn plant is randomly selected in each processing in picking front and back system
It counts fruiting sum and anti-fall voltage is total, calculate rate resistant to lodging.
2 results and analysis
The influence of 2.1 pairs of corn rates resistant to lodging
Through calculating, every 100 square meter plants 300 plants of corns and has used the fruit corn of the liquid fertilizer to fall as shown in Table 1
Volt rate only 0.06%, and control group is up to 9.3%；The lodging rate in seedling period is zero, and effect resistant to lodging is considerable, anti-fall
Volt effect is far longer than comparative example group.
Fruiting number of the table 1 to fruit corn plant different disposal, anti-fall voltage situation result
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (8)
1. a kind of liquid fertilizer resistant to lodging, which is characterized in that the liquid fertilizer is by following material composition: active liquid fertilizer base
1000 parts of bottom preparation, multiple 0.1 ~ 5 part of phthalein nucleic acid, 12 ~ 28 parts of Water-soluble silicon fertilizer, 52 ~ 70 parts of urea, amino acid 1 ~ 10 part, sulfuric acid
52 ~ 72 parts of potassium, 10 ~ 25 parts of calcium superphosphate, 1 ~ 10 part of boric acid, 0.1 ~ 5 part of white vitriol, total nitrogen content in the liquid fertilizer
>=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 110 ~ 145g/L；
The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；Streptomyces bacterial strain " TY-
The culture medium of 001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, be beaten, filtered；Then into filtrate
After sugar is added, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
2. liquid fertilizer resistant to lodging according to claim 1, which is characterized in that the liquid fertilizer is by following substance group
At: 1000 parts of active liquid fertilizer substrate preparation, multiple 1 ~ 3 part of phthalein nucleic acid, 15 ~ 22 parts of Water-soluble silicon fertilizer, 59 ~ 66 parts of urea, amino
Sour 3 ~ 8 parts, 58 ~ 68 parts of potassium sulfate, 13 ~ 21 parts of calcium superphosphate, 3 ~ 8 parts of boric acid, 1 ~ 3 part of white vitriol；The liquid fertilizer
Middle total nitrogen content >=2.5mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter are 120 ~ 135g/L.
3. liquid fertilizer resistant to lodging according to claim 1, which is characterized in that the liquid fertilizer is by following substance group
At: 1000 parts of active liquid fertilizer substrate preparation, multiple 2 parts of phthalein nucleic acid, 19 parts of Water-soluble silicon fertilizer, 64 parts of urea, 6 parts of amino acid, sulphur
Sour potassium 64,18 parts of calcium superphosphate, 6 parts of boric acid, 2 parts of white vitriol, total nitrogen content >=2.5mg/g in the liquid fertilizer, ammonia
Base nitrogen content >=0.4mg/g, the content of organic matter are 126 ~ 131g/L.
4. liquid fertilizer resistant to lodging according to claim 1, which is characterized in that the weight of N, P, K in the liquid fertilizer
Than for 4 ~ 8:1:4 ~ 8, preferably 6:1:6.
5. liquid fertilizer resistant to lodging according to claim 1, which is characterized in that the amino acid be proline, lysine,
One or more of tryptophan, tyrosine, phenylalanine, aspartic acid.
6. liquid fertilizer resistant to lodging according to claim 1, which is characterized in that the Water-soluble silicon fertilizer is sodium metasilicate, silicon
Sour potassium crosses one of sodium disilicate and sodium metasilicate.
7. a kind of preparation method of liquid fertilizer resistant to lodging, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. the configuration of liquid fertilizer: toward the active liquid fertilizer substrate preparation add multiple phthalein nucleic acid, Water-soluble silicon fertilizer, urea,
Amino acid, potassium sulfate, calcium superphosphate, boric acid, white vitriol obtain the liquid fertilizer.
8. the preparation method of liquid fertilizer resistant to lodging according to claim 7, which is characterized in that the active liquid fertilizer base
Its amino-acid nitrogen content >=4.5g/L after bottom preparation is stored 15 days.
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| Application Number | Priority Date | Filing Date | Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611671A (en) * | 2022-09-26 | 2023-01-17 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611671A (en) * | 2022-09-26 | 2023-01-17 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
| CN115611671B (en) * | 2022-09-26 | 2023-10-27 | 安徽省农业科学院作物研究所 | Lodging-resistant fertilizer for wheat and preparation method thereof |
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Application publication date: 20190702 |