CN109761701A - A kind of liquid fertilizer and preparation method thereof effectively facilitating sweet precious watermelon growth of flower bud - Google Patents

Abstract

The invention discloses a kind of liquid fertilizers and preparation method thereof for effectively facilitating sweet precious watermelon growth of flower bud, which includes 1000 parts of active liquid fertilizer substrate preparation；32 ~ 48 parts of nitrogen, 5 ~ 16 parts of potassium, 11 ~ 23 parts of phosphorus, 10 ~ 20 parts of sulphur, 5 ~ 12 parts of boron, 3 ~ 9 parts of zinc, 0.1 ~ 5 part of manganese, 0.1 ~ 5 part of magnesium；Contain 0.1 ~ 5 part of diethyl aminoethyl hexanoate, 20 ~ 32 parts of glycine, 9 ~ 15 parts of methionine, 1 ~ 10 part of lysine, 10 ~ 18 parts of phenylalanine in the liquid fertilizer；The liquid fertilizer has not only effectively facilitated the flower bud development of sweet precious watermelon, while having selectivity to the bud differentiation of different melon vines, the most significant to the bud differentiation effect of sweet precious watermelon.

Description

A kind of liquid fertilizer and preparation method thereof effectively facilitating sweet precious watermelon growth of flower bud

Technical field

The invention belongs to the technical fields of liquid fertilizer, and it is raw to effectively facilitate sweet precious watermelon bud more particularly, to one kind Long liquid fertilizer and preparation method thereof.

Background technique

Sweet treasured watermelon also known as platform are black, young fruit period fruit face bottle green, and green color stripe is indistinctly presented, and there is white in mature consequence face Fruit powder.Single melon weighs 6~7 jin, and big person is up to 12 jin or so.Skin depth about 1.2~1.5, storage tolerance.Plant strain growth is medium, and branch is more, Fruit setting power is strong, is medium variety.Melon pulp large red, quality is fine and close, and sweet, matter is good.Seed is small, light brown, but quantity is more.Cause The easy branch of the kind, fruit setting is more, so being that macronutrient is needed to supply in cultivation.

Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto- Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline, Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..

Sweet treasured watermelon vine bud differentiation refers to that axis growing point mitogenetic blade, axillary bud out are changed into and differentiates inflorescence or flower Piece process, bud differentiation is the physiology and morphological landmarks changed from nutrient growth to reproductive growth.Bud differentiation directly affects To yielding positive results for plant, therefore flower bud differentiation period has directly established the base of fruiting period and harvest time to the nutritional supplementation of plant Plinth.Often occur that bud differentiation is slow, and branch is few during sweet precious growth of watermelon, causes result few, fruit is small.

Summary of the invention

The problem of watermelon bud differentiation precious the technical problem to be solved by the present invention is to overcome existing honey, provides a kind of effectively rush Into the liquid fertilizer and preparation method thereof of sweet precious watermelon growth of flower bud.

Above-mentioned purpose of the present invention is achieved through the following technical solutions:

A kind of liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud, by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；32~48 parts of nitrogen, 5~16 parts of potassium, 11~23 parts of phosphorus, 10~20 parts of sulphur, 5~12 parts of boron, 3~9 parts of zinc, 0.1~5 part of manganese, 0.1~5 part of magnesium；

Contain 0.1~5 part of diethyl aminoethyl hexanoate, 20~32 parts of glycine, 9~15 parts of methionine, lysine 1 in the liquid fertilizer ~10 parts, 10~18 parts of phenylalanine；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >= 120g/L。

The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation.

The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, into Row mashing, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~ 7.6。

The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017 The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

Further, the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud, by the substance group of following parts by weight At:

1000 parts of active liquid fertilizer substrate preparation；36~44 parts of nitrogen, 8~12 parts of potassium, 15~18 parts of phosphorus, 13~17 parts of sulphur, 8~10 parts of boron, 4~7 parts of zinc, 0.5~1.5 part of manganese, 0.5~1.5 part of magnesium；

Contain 1~3 part of diethyl aminoethyl hexanoate, 25~28 parts of glycine, 11~13 parts of methionine, lysine 3 in the liquid fertilizer ~8 parts, 13~15 parts of phenylalanine；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >= 120g/L。

Preferably, the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud, by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；40 parts of nitrogen, 10 parts of potassium, 16 parts of phosphorus, 15 parts of sulphur, 9 parts of boron, 6 parts of zinc, manganese 1 Part, 1 part of magnesium；

Contain 2 parts of diethyl aminoethyl hexanoate, 26 parts of glycine, 12 parts of methionine, 5 parts of lysine, phenylalanine in the liquid fertilizer 14 parts；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

Further, the nitrogen, potassium, phosphorus portion rate be 4:1:1~2.

Further, the ratio between the sulphur, boron, zinc are 5:3:2.

Further, the ratio of the lysine and methionine is 1:2~3.

Further, the source of the sulphur includes sulphur, calcium sulfate, potassium sulfate, vulcanized sodium, ammonium sulfide, liquid titanium dioxide Sulphur.

A kind of preparation method of the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud, comprising the following steps:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C After it, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. the configuration of liquid fertilizer: toward active liquid fertilizer substrate preparation addition diethyl aminoethyl hexanoate, glycine, methionine, rely One or more of propylhomoserin, phenylalanine or nitrogen, potassium, phosphorus, sulphur, boron, zinc, manganese, magnesium obtain the liquid fertilizer；

The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017 The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

Further, the pre-fermented step are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e. It can；

S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.

Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.

Preferably, its amino-acid nitrogen content >=4.2g/L after the active liquid fertilizer substrate preparation is stored 15 days.

Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is 165g:250L。

Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge, Show there is condensed milk shape gloss；Spore Gram's staining is positive.

A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:

Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7 It, is made bacterial strain after activation；

Y2. strain inoculated is 25~30 DEG C, revolution in temperature into fluid nutrient medium after taking step S1 activation obtained Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.

Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.

Compared with prior art, the invention has the following advantages:

Liquid fertilizer of the invention can effectively facilitate the bud differentiation of sweet precious watermelon, and root growth significantly improves, and has Conducive to the quality of fruit and the nutrient growth of fruit tree.

Liquid fertilizer of the invention increases the new-tip length and rugosity all remarkable effects, length of sweet precious watermelon 27.3%, rugosity increases 20.3%.In terms of bud rate, which increases the bud rate promotion of sweet precious watermelon 15.7%, flower number increases 40.0%, achieves unexpected technical effect.However, the liquid fertilizer is to early spring carbuncle The new-tip length and bud rate of watermelon all do not play substantive effect.As it can be seen that liquid fertilizer of the invention is to different watermelons Bud differentiation has selectivity, the most significant to the bud differentiation effect of sweet precious watermelon, while to the strong plantlets and rootage of sweet precious watermelon With very significant effect, to the melon vine of different cultivars selectivity with higher.Streptomyces bacterial strain provided by the invention " TY-001# ", the bacterial strain can adapt to aerobic fementation culture under hyperosmosis.

Specific embodiment

The present invention is further illustrated below in conjunction with specific embodiment, but embodiment does not do any form to the present invention Restriction.Unless stated otherwise, the present invention uses reagent, method and apparatus for the art conventional reagent, method and are set It is standby.

Unless stated otherwise, following embodiment agents useful for same and material are commercially available.

The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)

1. experimental material and condition

(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.

(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1 It rises, pH 7.5.

Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15~ 25g, water are settled to 1 liter, pH 7.5.

Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).

(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation Equal operations carry out in superclean bench.

(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.

2. strain isolation and screening

(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.

(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to It is saved in case.

(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, 220 revs/min on oscillation shaking table, 25 DEG C of conditions Lower culture 5 days.

(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.

(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most Good surviving colonies access solid medium squamous subculture.

(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.

(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains (>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".

Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar 100g is settled to 1000mL, pH 7.5.

3. the identification of bacterial strain " TY-001# "

(1) Morphological Identification

Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining It is positive.

Morphological Identification shows that the bacterial strain belongs to streptomyces.

In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017 Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro- Biological five building, institute's laboratory building.

The embodiment 2 a kind of preparation and application of the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud

A kind of preparation method for the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 2 parts of diethyl aminoethyl hexanoate, glycine 26 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 12 parts of methionine, 5 parts of lysine, 14 parts of phenylalanine, 40 parts of nitrogen, 10 parts of potassium, 16 parts of phosphorus, 15 parts of sulphur, 9 parts of boron, 6 parts of zinc, 1 part of manganese, 1 part of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.Wherein N, P, K, S, B, Zn, Mn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, boric acid, zinc chloride, MnEDTA, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

The embodiment 3 a kind of preparation and application of the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud

A kind of preparation method for the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud the following steps are included:

S1. it prepares culture medium: after taking 1000g dregs of beans to carry out pre fermentation, being put into fiberizer and be beaten, filter, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

Pre-fermented step are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e. It can；

S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 4 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, temperature inside fermentation raw material At >=40 DEG C of degree, pre fermentation dregs of beans is inside and outside overturn once, continues fermentation 2 days；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 0.1 part of diethyl aminoethyl hexanoate, glycine the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations 20 parts, 15 parts of methionine, 7 parts of lysine, 10 parts of phenylalanine, 32 parts of nitrogen, 8 parts of potassium, 16 parts of phosphorus, sulphur 20,12 parts of boron, 8 parts of zinc, 5 parts of manganese, 5 parts of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L；Wherein N, P, K, S, B, Zn, Mn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, boric acid, zinc chloride, MnEDTA, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

The embodiment 4 a kind of preparation and application of the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud

A kind of preparation method for the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 0.1 part of diethyl aminoethyl hexanoate, glycine the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations 20 parts, 9 parts of methionine, 3 parts of lysine, 10 parts of phenylalanine, 32 parts of nitrogen, 8 parts of potassium, 11 parts of phosphorus, 10 parts of sulphur, 6 parts of boron, 4 parts of zinc, 0.1 part of manganese, 0.1 part of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.Wherein N, P, K, S, B, Zn, Mn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, boric acid, zinc chloride, MnEDTA, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

The embodiment 5 a kind of preparation and application of the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud

A kind of preparation method for the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 5 parts of diethyl aminoethyl hexanoate, glycine 32 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 15 parts of methionine, 7.5 parts of lysine, 18 parts of phenylalanine, 48 parts of nitrogen, 12 parts of potassium, 23 parts of phosphorus, 20 parts of sulphur, 12 parts of boron, zinc 8 parts, 5 parts of manganese, 5 parts of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.Wherein N, P, K, S, B, Zn, Mn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, boric acid, zinc chloride, MnEDTA, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

A kind of preparation for the liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud of comparative example 1

A kind of preparation method for the liquid fertilizer effectively facilitating sweet precious watermelon growth of flower bud the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation.

S4. 3 parts of diethyl aminoethyl hexanoate, glycine 23 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 10 parts of methionine, 5 parts of lysine, 15 parts of phenylalanine, 36 parts of nitrogen, 10 parts of potassium, 23 parts of phosphorus, 10 parts of sulphur, 10 parts of boron, zinc 5 Part, 3 parts of manganese, 3 parts of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.

Effectively facilitate the application effect appraisal of the liquid fertilizer of sweet precious watermelon growth of flower bud:

Nutrient needed for liquid fertilizer can quickly supplement the growth of fruit rattan promotes the precious watermelon vine bud differentiation of honey.Originally for verifying The actual effect of liquid fertilizer large-scale demonstration application on sweet precious watermelon fruit rattan of preparation is invented, applicant selects in sweet Bao Xi Experiment and demonstration has been carried out on melon.

1 materials and methods

1.1 are located in the precious watermelon base of Zixing City's honey for examination place 1,12 mu of testing ground area, soil fertility level compared with Uniformly.

For examination place 2 be located in Jianning city early spring carbuncle watermelon base, 12 mu of testing ground area, soil fertility level compared with Uniformly.

1.2 for studying the precious watermelon of the honey of object 1, rattan line-spacing 0.6m*1m, the growing way golden mean of the Confucian school and consistent.

For studying object 2 early spring carbuncle watermelon, rattan line-spacing 0.6m*1m, the growing way golden mean of the Confucian school and consistent.

1.3 for trying the liquid fertilizer that fertilizer is the precious watermelon bud differentiation of honey made from embodiment 2~5, and product forms are liquid Body.

1.4 it is each for examination place carry out 3 test process:

1. experimental group: it pours within every 3 months after plantation and applies once for trying fertilizer, it is 500mL/ mus each, dilute 200 times.

2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing ①。

3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied The same processing of fertile measure is 1..

Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre It for cell, is repeated 3 times, management is consistent.Each group randomly selects 10 plants of melon vine measurement bud differentiation situations, acquires average value.

2 results and analysis

2.1 different disposals are to young sprout growth, the influence of bud rate and flower number

As shown in Table 1, after applying the liquid fertilizer, the new-tip length and rugosity of watermelon are significantly higher than control group, length 27.3% is increased, rugosity increases 20.3%.In terms of bud rate, which increases the bud rate of watermelon 15.7%, flower number increases 40.0%, achieves unexpected technical effect.However, the liquid fertilizer is to early spring carbuncle The growth of flower bud of watermelon does not all play substantive effect.As it can be seen that the bud differentiation of liquid fertilizer of the invention to different melon vines It is the most significant to the bud differentiation effect of sweet precious watermelon with selectivity.

Table 1

The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification, Equivalent replacement, improvement etc. should be included within scope of the invention.

Claims (10)

1. a kind of liquid fertilizer for effectively facilitating sweet precious watermelon growth of flower bud, which is characterized in that by the substance group of following parts by weight At:

1000 parts of active liquid fertilizer substrate preparation；32 ~ 48 parts of nitrogen, 5 ~ 16 parts of potassium, 11 ~ 23 parts of phosphorus, 10 ~ 20 parts of sulphur, 5 ~ 12 parts of boron, 3 ~ 9 parts of zinc, 0.1 ~ 5 part of manganese, 0.1 ~ 5 part of magnesium；

In the liquid fertilizer containing 0.1 ~ 5 part of diethyl aminoethyl hexanoate, 20 ~ 32 parts of glycine, 9 ~ 15 parts of methionine, 1 ~ 10 part of lysine, 10 ~ 18 parts of phenylalanine；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

2. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that by following heavy Measure the material composition of part:

1000 parts of active liquid fertilizer substrate preparation；36 ~ 44 parts of nitrogen, 8 ~ 12 parts of potassium, 15 ~ 18 parts of phosphorus, 13 ~ 17 parts of sulphur, 8 ~ 10 parts of boron, 4 ~ 7 parts of zinc, 0.5 ~ 1.5 part of manganese, 0.5 ~ 1.5 part of magnesium；

Contain 1 ~ 3 part of diethyl aminoethyl hexanoate, 25 ~ 28 parts of glycine, 11 ~ 13 parts of methionine, 3 ~ 8 parts of lysine, benzene in the liquid fertilizer 13 ~ 15 parts of alanine；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

3. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that by following heavy Measure the material composition of part:

1000 parts of active liquid fertilizer substrate preparation；40 parts of nitrogen, 10 parts of potassium, 16 parts of phosphorus, 15 parts of sulphur, 9 parts of boron, 6 parts of zinc, 1 part of manganese, magnesium 1 part；

Contain 2 parts of diethyl aminoethyl hexanoate, 26 parts of glycine, 12 parts of methionine, 5 parts of lysine, 14 parts of phenylalanine in the liquid fertilizer； Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

4. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that the activity Liquid Fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；

The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, beaten Slurry, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

5. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that the nitrogen, Potassium, phosphorus portion rate be 4:1:1 ~ 2.

6. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that the sulphur, The ratio between boron, zinc are 5:3:2.

7. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that the bad ammonia The ratio of acid and methionine is 1:2 ~ 3.

8. effectively facilitating the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 1, which is characterized in that the sulphur Source includes sulphur, calcium sulfate, potassium sulfate, vulcanized sodium, ammonium sulfide, liquid sulfur dioxide.

9. effectively facilitating the preparation method of the liquid fertilizer of sweet precious watermelon growth of flower bud described in one kind, which is characterized in that including with Lower step:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain To fermentation liquid；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. the configuration of liquid fertilizer: toward active liquid fertilizer substrate preparation addition diethyl aminoethyl hexanoate, glycine, methionine, rely ammonia One or more of sour, phenylalanine or nitrogen, potassium, phosphorus, sulphur, boron, zinc, manganese, magnesium, obtain the liquid fertilizer；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.

10. effectively facilitating the preparation method of the liquid fertilizer of sweet precious watermelon growth of flower bud according to claim 9, feature exists In its amino-acid nitrogen content >=4.2g/L after the active liquid fertilizer substrate preparation is stored 15 days.