CN109761689A - A kind of liquid fertilizer and preparation method thereof effectively improving sweet precious Watermelon Fruit quality - Google Patents

Abstract

The invention discloses a kind of liquid fertilizers and preparation method thereof for effectively improving sweet precious Watermelon Fruit quality, which includes 1000 parts of active liquid fertilizer substrate preparation；22 ~ 38 parts of nitrogen, 22 ~ 36 parts of potassium, 10 ~ 20 parts of phosphorus, 11 ~ 20 parts of sulphur, 0.1 ~ 5 part of calcium, 6 ~ 15 parts of boron, 0.05 ~ 3 part of zinc, 0.05 ~ 3 part of manganese, 0.05 ~ 3 part of magnesium；Contain 0.5 ~ 6 part of diethyl aminoethyl hexanoate, 22 ~ 35 parts of glycine, 20 ~ 30 parts of proline, 6 ~ 16 parts of lysine, 7 ~ 18 parts of phenylalanine in the liquid fertilizer；The fruit quality that the liquid fertilizer not only effectively facilitates sweet precious watermelon improves, and increases fruit yield, while having selectivity to the effect of different melon vines, the most significant to the effect of sweet precious watermelon.

Description

A kind of liquid fertilizer and preparation method thereof effectively improving sweet precious Watermelon Fruit quality

Technical field

The invention belongs to the technical fields of liquid fertilizer, effectively improve sweet precious Watermelon Fruit product more particularly, to one kind Liquid fertilizer of matter and preparation method thereof.

Background technique

Sweet treasured watermelon also known as platform are black, young fruit period fruit face bottle green, and green color stripe is indistinctly presented, and there is white in mature consequence face Fruit powder.Single melon weighs 6~7 jin, and big person is up to 12 jin or so.Skin depth about 1.2~1.5, storage tolerance.Plant strain growth is medium, and branch is more, Fruit setting power is strong, is medium variety.Melon pulp large red, quality is fine and close, and sweet, matter is good.Seed is small, light brown, but quantity is more.Cause The easy branch of the kind, fruit setting is more, so being that macronutrient is needed to supply in cultivation.

Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto- Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline, Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..

Partially to fertilize, especially inclined nitrogen fertilizer application easily lacks honey along with land occupation condition is poor for traditional fertilising habit happiness The elements such as the big phosphorus potassium calcium of precious watermelon vine body demand, are easier to cause the shortage of micronutrient boron, zinc, iron, magnesium, are influencing plant just Normal growth and development causes sweet precious Watermelon Fruit small, and phenomena such as berry decayed fruit, dehiscent fruit, fruit russeting occurs in low output.

Summary of the invention

The problem of quality of watermelon precious the technical problem to be solved by the present invention is to overcome existing honey, one kind is provided and effectively improves honey The liquid fertilizer and preparation method thereof of precious Watermelon Fruit quality.

Above-mentioned purpose of the present invention is achieved through the following technical solutions:

A kind of liquid fertilizer effectively improving sweet precious Watermelon Fruit quality, by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；22~38 parts of nitrogen, 41~58 parts of potassium, 1~10 part of phosphorus, 8~16 parts of sulphur, calcium 0.1~5 part, 8~16 parts of boron, 0.01~3 part of zinc, 0.01~3 part of magnesium；

Contain 0.5~10 part of diethyl aminoethyl hexanoate, 30~41 parts of glycine, 1~12 part of proline, tryptophan in the liquid fertilizer 21~32 parts, 3~12 parts of aspartic acid；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >= 120g/L。

The active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation.

The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, into Row mashing, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~ 7.6。

The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017 The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide 5 building, the building of compound the 59th of state city martyr Road 100.

Further, the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality, by the substance group of following parts by weight At:

1000 parts of active liquid fertilizer substrate preparation；25~32 parts of nitrogen, 46~53 parts of potassium, 2~5 parts of phosphorus, 11~14 parts of sulphur, 0.5~2 part of calcium, 10~13 parts of boron, 0.1~1 part of zinc, 0.1~1 part of magnesium；

In the liquid fertilizer containing 3~6 parts of diethyl aminoethyl hexanoate, 33~38 parts of glycine, proline-4~8 part, tryptophan 24~ 28 parts, 6~9 parts of aspartic acid；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/ L。

Preferably, the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality, by the material composition of following parts by weight:

1000 parts of active liquid fertilizer substrate preparation；30 parts of nitrogen, 51 parts of potassium, 3 parts of phosphorus, 12 parts of sulphur, 1 part of calcium, 12 parts of boron, zinc 0.5 part, 0.5 part of magnesium；

Contain 5 parts of diethyl aminoethyl hexanoate, 35 parts of glycine, 5 parts of proline, 26 parts of tryptophan, aspartic acid in the liquid fertilizer 8 parts；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

Further, the nitrogen, phosphorus, potassium portion rate be 10:1:15~18.

Further, the ratio between the calcium, boron, sulphur are 1:12:12.

Further, the ratio of the diethyl aminoethyl hexanoate, glycosides propylhomoserin and tryptophan is 1:7:5~7.

Further, the source of the sulphur includes sulphur, calcium sulfate, potassium sulfate, vulcanized sodium, ammonium sulfide, liquid titanium dioxide Sulphur.

Further, the source of the calcium includes calcium sulfate, calcium lactate, calcium gluconate, calcium citrate.

A kind of preparation method of the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality, comprising the following steps:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, the continuing fermentation 7~8 at 20~28 DEG C After it, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. diethyl aminoethyl hexanoate, glycine, proline, color the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation One or more of propylhomoserin, aspartic acid or nitrogen, potassium, phosphorus, sulphur, calcium, boron, zinc, magnesium obtain the liquid fertilizer；

The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017 The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide 5 building, the building of compound the 59th of state city martyr Road 100.

Further, the pre-fermented step are as follows:

S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e. It can；

S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；

S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.

Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.

Preferably, its amino-acid nitrogen content >=4.2g/L after the active liquid fertilizer substrate preparation is stored 15 days.

Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is 165g:250L。

Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge, Show there is condensed milk shape gloss；Spore Gram's staining is positive.

A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:

Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7 It, is made bacterial strain after activation；

Y2. strain inoculated is into fluid nutrient medium after taking step S1 activation obtained, temperature is 25~30 DEG C, revolution is Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.

Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, Water is settled to 1 liter, and pH is 7.4~7.6.

Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.

Compared with prior art, the invention has the following advantages:

The fruit quality that liquid fertilizer of the invention can effectively facilitate sweet precious watermelon improves, and is conducive to the growth of melon vine Development can effectively prevent sweet precious watermelon shedding, dehiscent fruit.

The precious Watermelon Fruit transverse diameter growth rate of honey for applying liquid fertilizer of the invention is up to 420.4%, is higher by than control group 30.3%.Single fresh fruit increases 28.1% again, realizes that per mu yield growth rate is up to 99.7%, effect is considerable.The liquid Body fertilizer improves a lot to the fruit quality of sweet precious watermelon, and soluble solid content increases 31.3%.However, the liquid Body fertilizer, which increases the fruit diameter growth rate, per mu yield and fruit quality of early spring carbuncle melon, only has lesser effect.As it can be seen that of the invention Liquid fertilizer there is very significant effect to the raising fruit quality of sweet precious watermelon, fruit is improved to the watermelon of different cultivars Quality fruit selectivity with higher.

Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train It supports.

Specific embodiment

The present invention is further illustrated below in conjunction with specification and specific embodiment, but embodiment does not appoint the present invention The restriction of what form.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, side Method and equipment.

Unless stated otherwise, following embodiment agents useful for same and material are commercially available.

The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)

1. experimental material and condition

(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.

(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1 It rises, pH 7.5.

Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar, Water is settled to 1 liter, pH 7.5.

Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).

(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation Equal operations carry out in superclean bench.

(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.

2. strain isolation and screening

(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.

(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to It is saved in case.

(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, 220 revs/min on oscillation shaking table, 25 DEG C of conditions Lower culture 5 days.

(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.

(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most Good surviving colonies access solid medium squamous subculture.

(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.

(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains (>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".

Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar 100g is settled to 1000mL, pH 7.5.

3. the identification of bacterial strain " TY-001# "

(1) Morphological Identification

Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining It is positive.

Morphological Identification shows that the bacterial strain belongs to streptomyces.

In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017 Kind collection, deposit number are GDMCC No.:60152, and preservation address is Xianlie Mid Road, Guangzhou City, Guangdong Province, China 100 Number 5 building, the building of compound the 59th.

The embodiment 2 a kind of preparation and application of the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality

A kind of preparation method for the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 5 parts of diethyl aminoethyl hexanoate, glycine 35 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 5 parts of proline, 26 parts of tryptophan, 8 parts of aspartic acid, 30 parts of nitrogen, 51 parts of potassium, 3 parts of phosphorus, 12 parts of sulphur, 1 part of calcium, 12 parts of boron, 0.5 part of zinc, 0.5 part of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.Wherein N, P, K, S, Ca, B, Zn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, calcium gluconate, boric acid, zinc chloride, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The embodiment 3 a kind of preparation and application of the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality

A kind of preparation method for the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 4.5 parts of diethyl aminoethyl hexanoate, glycine the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations 31.5 parts, 1 part of proline, 22.5 parts of tryptophan, 3 parts of aspartic acid, 24 parts of nitrogen, 41 parts of potassium, 2.4 parts of phosphorus, 9 parts of sulphur, calcium 0.75 Part, 9 parts of boron, 0.01 part of zinc, 0.01 part of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, Amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.Wherein N, P, K, S, Ca, B, Zn, Mg are respectively derived from phosphoric acid Ammonium dihydrogen and amino acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, calcium gluconate, boric acid, zinc chloride, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The embodiment 4 a kind of preparation and application of the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality

A kind of preparation method for the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 6 parts of diethyl aminoethyl hexanoate, glycine 42 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 5 parts of proline, 30 parts of tryptophan, 9 parts of aspartic acid, 30 parts of nitrogen, 45 parts of potassium, 3 parts of phosphorus, 15 parts of sulphur, 1.25 parts of calcium, boron 15 Part, 1 part of zinc, 1 part of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.9mg/g in gained liquid fertilizer, amino nitrogen content >= 0.78mg/g, the content of organic matter >=130g/L.Wherein N, P, K, S, Ca, B, Zn, Mg are respectively derived from ammonium dihydrogen phosphate and amino Acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, calcium gluconate, boric acid, zinc chloride, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The embodiment 5 a kind of preparation and application of the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality

A kind of preparation method for the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；

S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.

S5. 5 parts of diethyl aminoethyl hexanoate, glycine 35 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 12 parts of proline, 32 parts of tryptophan, 12 parts of aspartic acid, 38 parts of nitrogen, 57 parts of potassium, 3.8 parts of phosphorus, 16 parts of sulphur, 1.33 parts of calcium, 16 parts of boron, 3 parts of zinc, 3 parts of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g, ammonia nitrogen contain in gained liquid fertilizer Amount >=0.68mg/g, the content of organic matter >=140g/L.Wherein N, P, K, S, Ca, B, Zn, Mg be respectively derived from ammonium dihydrogen phosphate and Amino acid, ammonium dihydrogen phosphate, potassium sulfate, potassium sulfate, calcium gluconate, boric acid, zinc chloride, magnesium sulfate.

Streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

The comparative example 1 a kind of preparation and application of the liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality

A kind of preparation method for the liquid fertilizer effectively improving sweet precious Watermelon Fruit quality the following steps are included:

S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium L, pH 7.5；

S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C After supervention ferment 7 days, fermentation liquid is obtained；

S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation.

S4. 10 parts of diethyl aminoethyl hexanoate, glycine 41 configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations Part, 6 parts of proline, 30 parts of tryptophan, 12 parts of aspartic acid, 30 parts of nitrogen, 40 parts of potassium, 5 parts of phosphorus, 9 parts of sulphur, 3 parts of calcium, 9 parts of boron, zinc 2 parts, 2 parts of magnesium, obtain the liquid fertilizer；Total nitrogen content >=2.7mg/g in gained liquid fertilizer, amino nitrogen content >= 0.68mg/g, the content of organic matter >=120g/L.

Effectively improve the application effect appraisal of the liquid fertilizer of sweet precious Watermelon Fruit quality:

Nutrient needed for Amino acid liquid fertilizer can quickly supplement the growth of fruit rattan promotes melon and fruit growth, improves Watermelon Fruit Mouthfeel and quality.For the practical effect for verifying liquid fertilizer prepared by the present invention large-scale demonstration application on sweet precious watermelon fruit rattan Fruit, applicant's selection have carried out experiment and demonstration on sweet precious watermelon.

1 materials and methods

1.1 are located in the precious watermelon base of Zixing City's honey for examination place 1,12 mu of testing ground area, soil fertility level compared with Uniformly.

For examination place 2 be located in Jianning city early spring carbuncle watermelon base, 12 mu of testing ground area, soil fertility level compared with Uniformly.

1.2 for studying the precious watermelon of the honey of object 1, rattan line-spacing 0.6m*1m, the growing way golden mean of the Confucian school and consistent.

For studying object 2 early spring carbuncle watermelon, rattan line-spacing 0.6m*1m, the growing way golden mean of the Confucian school and consistent.

1.3 for examination fertilizer be the liquid fertilizer that sweet precious Watermelon Fruit quality is effectively improved made from embodiment 2~5, product Dosage form is liquid.

1.4 it is each for examination place carry out 3 test process:

1. experimental group: it pours within every 3 months after plantation and applies once for trying fertilizer, it is 500mL/ mus each, dilute 200 times.

2. control group (CK): per acre every time with the progress of same experimental group equivalent clear water same time, other measures of fertilizer are the same as processing ①。

3. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied The same processing of fertile measure is 1..

Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre It for cell, is repeated 3 times, management is consistent.Determine that 10 plants of melon vines are adjusted when the first fringe fruit is expanded and before harvesting in each processing at random Look into the average value of fruit size, statistics per mu yield, each processing fruit quality of detection.

2 results and analysis

The influence of 2.1 pairs of fruit transverse diameters

As shown in Table 1, the precious Watermelon Fruit transverse diameter growth rate of honey for applying the liquid fertilizer is up to 420.4%, compares control group It has been higher by 30.3%, has been higher by 13.2% than comparative example group.However, comparing early spring carbuncle watermelon it is found that applying the Liquid Fertilizer Expect smaller for the facilitation of the transverse diameter ratio of fruit.

1 fruit transverse diameter measurement result of table

The influence of 2.2 pairs of per mu yields

Strain number, random 10 points of samplings count total fruiting number, claim fresh fruit weight, calculate total fruiting number, single fresh fruit statistics per acre The average value of weight calculates theoretical per mu yield.

Through calculating, 1000 plants of watermelon vines, the average value of fruiting number, single fresh fruit weight and per mu yield such as table 2 are planted per acre.By Table 2 is it is found that used the precious single fresh fruit of watermelon of the honey of the liquid fertilizer to increase 28.1% again, realization per mu yield growth rate is up to 99.7%, effect is considerable.However, the liquid fertilizer is only more small to fruit retention rate and the per mu yield increase of early spring carbuncle Effect.As it can be seen that liquid fertilizer of the invention, which increases the fruit of sweet precious watermelon, has very significant effect, to different cultivars Watermelon selectivity with higher.

Table 2

The influence of 2.3 pairs of soluble solid contents

It squeezes the juice after taking several fresh fruits to remove the peel at random, measures soluble fixture content, experimental group and control group respectively measure 5 It is secondary, it is averaged.Experimental result is as shown in table 3.

As shown in Table 3, which improves a lot to the fruit quality of watermelon, and soluble solid content is compared Control group increases 31.3%, increases 11% compared to comparative example group.However, simultaneously to the fruit quality facilitation effect of early spring carbuncle It is unobvious.

Table 3

Processing	Soluble solid content/%
		Sweet treasured watermelon experimental group	15.1
Sweet treasured watermelon control group	11.5
		Sweet treasured watermelon comparative example group	13.6
Early spring carbuncle watermelon experimental group	10.6
		Early spring carbuncle watermelon control group	9.9
Early spring carbuncle watermelon comparative example group	10.2

The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification, Equivalent replacement, improvement etc. should be included within scope of the invention.

Claims (10)

1. a kind of liquid fertilizer for effectively improving sweet precious Watermelon Fruit quality, which is characterized in that by the substance group of following parts by weight At: 1000 parts of active liquid fertilizer substrate preparation；22~38 parts of nitrogen, 41~58 parts of potassium, 1~10 part of phosphorus, 8~16 parts of sulphur, calcium 0.1~ 5 parts, 8~16 parts of boron, 0.01~3 part of zinc, 0.01~3 part of magnesium；

In the liquid fertilizer containing 0.5~10 part of diethyl aminoethyl hexanoate, 30~41 parts of glycine, 1~12 part of proline, tryptophan 21~ 32 parts, 3~12 parts of aspartic acid；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/ L。

2. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that by following heavy Measure the material composition of part:

1000 parts of active liquid fertilizer substrate preparation；25~32 parts of nitrogen, 46~53 parts of potassium, 2~5 parts of phosphorus, 11~14 parts of sulphur, calcium 0.5 ~2 parts, 10~13 parts of boron, 0.1~1 part of zinc, 0.1~1 part of magnesium；

Contain 3~6 parts of diethyl aminoethyl hexanoate, 33~38 parts of glycine, proline-4~8 part, tryptophan 24~28 in the liquid fertilizer Part, 6~9 parts of aspartic acid；Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

3. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that by following heavy Measure the material composition of part:

1000 parts of active liquid fertilizer substrate preparation；30 parts of nitrogen, 51 parts of potassium, 3 parts of phosphorus, 12 parts of sulphur, 1 part of calcium, 12 parts of boron, 0.5 part of zinc, 0.5 part of magnesium；

Contain 5 parts of diethyl aminoethyl hexanoate, 35 parts of glycine, 5 parts of proline, 26 parts of tryptophan, 8 parts of aspartic acid in the liquid fertilizer； Total nitrogen content >=2.7mg/g, amino nitrogen content >=0.68mg/g, the content of organic matter >=120g/L.

4. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that the activity Liquid Fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；

The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, beaten Slurry, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

5. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that the nitrogen, Phosphorus, potassium portion rate be 10:1:15~18.

6. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that the calcium, The ratio between boron, sulphur are 1:12:12.

7. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that the amine is fresh The ratio of ester, glycosides propylhomoserin and tryptophan is 1:7:5~7.

8. effectively improving the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 1, which is characterized in that the sulphur Source includes sulphur, calcium sulfate, potassium sulfate, vulcanized sodium, ammonium sulfide, liquid sulfur dioxide.

9. effectively improving the preparation method of the liquid fertilizer of sweet precious Watermelon Fruit quality described in one kind, which is characterized in that including with Lower step:

S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filtrate After middle addition sugar, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；

S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；

S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20~28 DEG C after continuing fermentation 7~8 days, Obtain fermentation liquid；

S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；

S5. diethyl aminoethyl hexanoate, glycine, proline, color ammonia the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation One or more of sour, aspartic acid or nitrogen, potassium, phosphorus, sulphur, calcium, boron, zinc, magnesium, obtain the liquid fertilizer；

The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city 5 building, the building of compound the 59th of martyr Road 100.

10. effectively improving the preparation method of the liquid fertilizer of sweet precious Watermelon Fruit quality according to claim 9, feature exists In its amino-acid nitrogen content >=4.2g/L after the active liquid fertilizer substrate preparation is stored 15 days.