CN106399160B - A kind of acid soil vigor remediation microbial inoculum and preparation method thereof - Google Patents
A kind of acid soil vigor remediation microbial inoculum and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of acid soil vigor remediation microbial inoculum.The effective component of the microbial inoculum is streptomyces fradiae (Streptomyces Fradiae) and bacillus licheniformis (Bacillus Licheniformis).It is preferred that effective component is the bacillus licheniformis CLIMAX-VBL of streptomyces fradiae CLIMAX-VSF and deposit number the CGMCC No.12499 of deposit number CGMCC No.5133.The present invention also provides the preparation methods of microbial inoculum.This product is made of high-activity microorganism, basic materials (such as sodium metasilicate and potassium fulvate) and high-quality organic matter, it can be effectively improved and improve acid soil, significant Crop Improvement rhizosphere soil micro-ecological environment, enable microorganism rapid field planting and forms dominant microflora, nutritional ingredient is provided for plant growth, is created favorable conditions.
Description
Technical field
The present invention relates to agricultural microorganism preparation and soil fertilizer fields, specifically, being related to a kind of acid soil vigor
Remediation microbial inoculum and preparation method thereof.
Background technique
Agricultural production needs soil to provide the nutriment and living environment of plant growth.The soil life complicated as one
State system, for many years because of the use of unreasonable fertilizer and pesticide, so that soil acidification, hardened, micro organism quantity decline, organic matter
Content decline, soil-borne disease are got worse, so that the healthy growth to crops has an adverse effect.The study found that passing through
The microbial flora and its nutritional ingredient progress that input soil is reasonably combined, pH value is adjusted to 8-9, root of the crop will be obviously improved
Border soil microenvironment, significant rehabilitating soil vigor promote crop yield and quality.
Summary of the invention
The object of the present invention is to provide a kind of novel acid soil vigor remediation microbial inoculums and preparation method thereof.
In order to achieve the object of the present invention, the present invention provides a kind of acid soil vigor remediation microbial inoculum, and effective component is not
Family name streptomycete (Streptomyces Fradiae) and bacillus licheniformis (Bacillus Licheniformis).Preferably,
The effective component of the remediation microbial inoculum is the streptomyces fradiae (Streptomyces that deposit number is CGMCC No.5133
Fradiae) bacillus licheniformis (the Bacillus that CLIMAX-VSF and deposit number are CGMCC No.12499
Licheniformis)CLIMAX-VBL。
Wherein, streptomyces fradiae CLIMAX-VSF is isolated from the soil of high-yield field, which very significantly resists
Disease, fixing nitrogen, dissolving phosphor and dissolving potassium and secretion auxin performance.In China Committee for Culture Collection of Microorganisms's common micro-organisms
The heart carries out preservation, and preservation accession designation number is CGMCC No.5133, and the deposit date is on August 12nd, 2011.
Bacillus licheniformis (Bacillus Licheniformis) CLIMAX-VBL is divided from crop root part soil
From, purification, rejuvenation and obtain, which can significantly improve crop yield and anti-adversity ability, significant decrease crop disease.Bacterial strain
CLIMAX-VBL has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address Beijing southern exposure
The institute 3 of area North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.12499,
Preservation date on May 25th, 2016.
Acid soil vigor remediation microbial inoculum provided by the invention, the component containing following parts by weight: streptomyces fradiae microbial inoculum
5-10 parts, 5-10 parts of bacillus licheniformis agent, 10-20 parts of potassium fulvate, 20-40 parts of humic acid miberal powder, leached tea oil slag 10-20
Part, 5-10 parts of cigarette stalk powder, 3-6 parts of powdery sodium silicate, 2-5 parts of monohydrate zinc sulphate, 1-3 parts and zeolite powder 10-20 parts of borax.
Preferably, containing 8 parts of streptomyces fradiae microbial inoculum, 8 parts of bacillus licheniformis agent, 15 parts of potassium fulvate in microbial inoculum,
30 parts of humic acid miberal powder, 15 parts of leached tea oil slag, 8 parts of cigarette stalk powder, 5 parts of powdery sodium silicate, 3 parts of monohydrate zinc sulphate, 2 parts of borax and zeolite
15 parts of powder.
Streptomyces fradiae and the viable count of bacillus licheniformis point in acid soil vigor remediation microbial inoculum provided by the invention
Not Wei 5,000,000,000/gram or more and 200,000,000/gram or more.
Acid soil vigor remediation microbial inoculum provided by the invention, can be prepared as follows: prepare Freund chain respectively
Mould microbial inoculum and bacillus licheniformis agent, then mixed in proportion with other ingredients to get.
The streptomyces fradiae microbial inoculum can be prepared as follows:
S11, streptomyces fradiae seed liquor is prepared；
The solid multiplication culture of S12, streptomyces fradiae: the seed liquor of step S11 is linked into solid multiplication culture medium,
Cultivated under 30 ± 2 DEG C of sterile aerobic conditions 7-10 days to viable count be 5,000,000,000/gram or more；The solid multiplication culture medium contains
There are 20-40 parts of 50-70 parts of wheat bran, 3-6 parts of brown sugar, cottonseed cake or soya-bean cake, 1-3 parts of magnesium sulfate, 1-3 parts of sodium chloride, calcium carbonate 1-4
Part, pH7.0-7.5；
S13, the culture medium after Multiplying culture is dried to get streptomyces fradiae microbial inoculum.
The bacillus licheniformis agent can be prepared as follows:
S21, the bacillus licheniformis being stored in freeze-drying pipe is linked into the solid slope of broth bouillon, in 30-
It is cultivated 24-48 hours at 32 DEG C；
In S22, picking colony access PDA solid slope, cultivated 3-7 days at 30-32 DEG C；
S23, picking colony are linked into the fluid nutrient medium by sterilizing, under the conditions of 30-32 DEG C, 150-200r/min
Culture 36-48 hours, obtains the fermentation liquid of bacillus licheniformis；
Wherein, the fluid nutrient medium contains the component of following parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, peptone 20
Part, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid is mixed with solid absorbent by the weight ratio of 1:4-8, carries out viable count detection after dry, i.e.,
?.
Wherein, the solid absorbent is at least one of turf, zeolite or calcium carbonate etc..
Powdery microbial inoculum product is made by the above process, can plough under soil with site preparation, or is applied or pouring root application in Stage of Top Dressing punching.
The present invention also provides the biological organic bacterial manure for containing the acid soil vigor remediation microbial inoculum.
The present invention also provides application of the acid soil vigor remediation microbial inoculum in preparation biological organic bacterial manure.
The present invention further provides the acid soil vigor remediation microbial inoculums in rehabilitating soil vigor and to improve soil fertilizer
Application in power.
Streptomyces fradiae CLIMAX-VSF generates Multiple Classes of Antibiotics, through many years of field application, even to bacillary, fungoid
It is that Viral plant disease has good prevention and control effect.In addition to this, which has outstanding fixed nitrogen, phosphorus decomposing, potassium decomposing
Can, the various plants auxin including GA3, IAA, CTK can be secreted, there is obvious make to crop nutrient absorption level is promoted
With；Bacillus licheniformis CLIMAX-VBL can be to ingredients such as exocytosis restraining epiphyte peptide, chitinases, to various plants soil-borne disease
Evil (especially fungal disease) has good prevention and control to act on.Both the above strain passes through a large amount of combine experiments, it was demonstrated that compound system
The sterile intermediate antagonism of agent, and the holding time reaches commercial requirements, properties of product obtain further adding in terms of anti-soil-borne disease
By force.On the basis of streptomyces fradiae CLIMAX-VSF, by the addition of bacillus licheniformis CLIMAX-VBL, is further promoted and made
Object resists soil-borne disease performance, and the stability of preparation further enhances (bacterial strain CLIMAX-VBL is more resistant to high temperature), by Freund chain
The composite bacteria agent of mould CLIMAX-VSF and bacillus licheniformis CLIMAX-VBL preparation has culture fertility, improves chemical fertilizer
Utilization rate inhibits absorption of the crop to nitrate nitrogen, heavy metal and pesticide, has significant purification and rehabilitating soil effect.
The invention has the following advantages that
(1) this product is made of high-activity microorganism, basic materials (such as sodium metasilicate and potassium fulvate) and high-quality organic matter,
It can be effectively improved and improve acid soil, significant Crop Improvement rhizosphere soil micro-ecological environment enables microorganism rapid field planting simultaneously
Dominant microflora is formed, nutritional ingredient is provided for plant growth, creates favorable conditions.
(2) two kinds of effective components -- streptomyces fradiae CLIMAX-VSF and bacillus licheniformis CLIMAX-VBL be not mutually short of money
Anti-, the two is used in compounding, and has significant synergistic effect.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
Unless otherwise noted, heretofore described " part " refers to " parts by weight ".
Streptomyces fradiae microbial inoculum involved in following embodiment can be prepared as follows:
S11, streptomyces fradiae CLIMAX-VSF seed liquor is prepared；
The solid multiplication culture of S12, streptomyces fradiae: the seed liquor of step S11 is linked into solid multiplication culture medium,
Cultivated under 30 ± 2 DEG C of sterile aerobic conditions 7-10 days to viable count be 5,000,000,000/gram or more；The solid multiplication culture medium contains
There are 20-40 parts of 50-70 parts of wheat bran, 3-6 parts of brown sugar, cottonseed cake or soya-bean cake, 1-3 parts of magnesium sulfate, 1-3 parts of sodium chloride, calcium carbonate 1-4
Part, pH7.0-7.5；
S13, the culture medium after Multiplying culture is dried to get streptomyces fradiae CLIMAX-VSF microbial inoculum.
Bacillus licheniformis agent involved in following embodiment can be prepared as follows:
S21, the solid that the bacillus licheniformis CLIMAX-VBL being stored in freeze-drying pipe is linked into broth bouillon are oblique
In face, cultivated 24-48 hours at 30-32 DEG C；
In S22, picking colony access PDA solid slope, cultivated 3-7 days at 30-32 DEG C；
S23, picking colony are linked into the fluid nutrient medium by sterilizing, under the conditions of 30-32 DEG C, 150-200r/min
Culture 36-48 hours, obtains the fermentation liquid of bacillus licheniformis；
Wherein, the fluid nutrient medium contains the component of following parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, peptone 20
Part, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid is mixed with solid absorbent by the weight ratio of 1:6, viable count detection is carried out after dry to get ground
Clothing bacillus CLIMAX-VBL microbial inoculum.
Wherein, the solid absorbent is turf and/or at least one of zeolite or calcium carbonate.
1 acid soil vigor remediation microbial inoculum of embodiment and preparation method thereof
Contain 8 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum in acid soil vigor remediation microbial inoculum provided in this embodiment,
8 parts of bacillus licheniformis CLIMAX-VBL microbial inoculum, 15 parts of potassium fulvate, 30 parts of humic acid miberal powder, 15 parts of leached tea oil slag, cigarette stalk powder 8
Part, 5 parts of powdery sodium silicate, 3 parts of monohydrate zinc sulphate, 2 parts and 15 parts of zeolite powder of borax.Wherein, streptomyces fradiae and lichens gemma
The viable count of bacillus is respectively 5,000,000,000/gram or more and 200,000,000/gram or more.
Remediation microbial inoculum in embodiment 1-4 can be prepared as follows: prepare streptomyces fradiae microbial inoculum and ground respectively
Clothing gemma bacillus agent, then mixed in proportion with other ingredients to get.
2 acid soil vigor remediation microbial inoculum of embodiment and preparation method thereof
Contain 5 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum in acid soil vigor remediation microbial inoculum provided in this embodiment,
5 parts of bacillus licheniformis CLIMAX-VBL microbial inoculum, 10 parts of potassium fulvate, 20 parts of humic acid miberal powder, 10 parts of leached tea oil slag, cigarette stalk powder 5
Part, 3 parts of powdery sodium silicate, 2 parts of monohydrate zinc sulphate, 1 part and 10 parts of zeolite powder of borax.Wherein, streptomyces fradiae and lichens gemma
The viable count of bacillus is respectively 5,000,000,000/gram or more and 200,000,000/gram or more.
3 acid soil vigor remediation microbial inoculum of embodiment and preparation method thereof
Contain 10 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum in acid soil vigor remediation microbial inoculum provided in this embodiment,
10 parts of bacillus licheniformis CLIMAX-VBL microbial inoculum, 20 parts of potassium fulvate, 40 parts of humic acid miberal powder, 20 parts of leached tea oil slag, cigarette stalk powder
10 parts, 6 parts of powdery sodium silicate, 5 parts of monohydrate zinc sulphate, 3 parts and 20 parts of zeolite powder of borax.Wherein, streptomyces fradiae and lichens bud
The viable count of spore bacillus is respectively 5,000,000,000/gram or more and 200,000,000/gram or more.
The application of 4 acid soil vigor remediation microbial inoculum of embodiment
Contain streptomyces fradiae CLIMAX-VSF and bacillus licheniformis in acid soil vigor remediation microbial inoculum of the invention
CLIMAX-VBL is verified by field trial, has very ideal using effect.
Wherein, streptomyces fradiae CLIMAX-VSF is that a kind of safety is very high, can be used for biological husbantry and bio-pharmaceuticals
Strain, the secretion various plants auxin such as GA3, IAA, CTK, and have significant controlling disease performance, can prevent and treat including
More than 30 Major Diseases such as damping-off, verticillium wilt, black spot, downy mildew, root rot, viral mosaic disease, the bacterium is simultaneously to line
Worm has inhibiting effect, has good volume increase and biological control performance.
Bacillus licheniformis CLIMAX-VBL is nontoxic to people and animals, and the speed of growth is fast, and nutritional need is simple, heat-resisting and anti-
Inverse property is strong, can be to ingredients such as exocytosis restraining epiphyte peptide, chitinases, to various plants soil-borne disease (especially fungoid
Disease) there are good prevention and control to act on.The bacterium is adaptable, performance stablize, have good promotion plant growth, culture fertility,
It improves chemical fertilizer utilization rate, inhibit absorption of the crop to nitrate nitrogen, heavy metal and pesticide, there is significant purification and repair soil
Earth effect.
Both the above microbial bacteria belongs to the safe biologic bacterium that biohazard degree is four classes and (under normal conditions will not
Cause the microorganism of mankind or animal disease), which analyzes by laboratory research, each other without Antagonistic reaction, can have
Effect improves and conditioning acid soil, significant Crop Improvement rhizosphere soil micro-ecological environment enable microorganism rapid field planting and formed
Dominant microflora provides nutritional ingredient for plant growth, creates favorable conditions.
Field trial control is as follows:
1, it cucumber: is applied per acre using the cave acid soil vigor remediation microbial inoculum 40kg of embodiment 1, output increased 19.2-
26.7%, cucumber fusarium axysporum mitigates 35.2-47.6% than compareing the plot not used, 5-7 days mature ahead of time.Dramatically increase kind
Plant benefit.
2, it green pepper: is applied per acre using the cave acid soil vigor remediation microbial inoculum 40kg of embodiment 1, output increased 16.1-
24.3%, Pepper Phytophthora Blight mitigates 44.5-59.4% than compareing the plot not used, 3-5 days mature ahead of time.
3, grape: the acid soil vigor remediation microbial inoculum 80kg ditch spread of embodiment 1, output increased 20.9- are used per acre
30.4%, 5-7 days mature ahead of time, the bright-coloured gloss of fruit color, sugariness increases by 2 degree or more, storage endurance.
The acid soil vigor remediation microbial inoculum of embodiment 2 and 3 has the effect of similar.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of acid soil vigor remediation microbial inoculum, which is characterized in that effective component be deposit number be CGMCC No.5133 not
The lichens gemma bar that family name streptomycete (Streptomyces Fradiae) CLIMAX-VSF and deposit number are CGMCC No.12499
Bacterium (Bacillus Licheniformis) CLIMAX-VBL；
The microbial inoculum contains the component of following parts by weight: 5-10 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum, bacillus licheniformis
5-10 parts of CLIMAX-VBL microbial inoculum, 10-20 parts of potassium fulvate, 20-40 parts of humic acid miberal powder, 10-20 parts of leached tea oil slag, cigarette stalk powder 5-
10 parts, 3-6 parts of powdery sodium silicate, 2-5 parts of monohydrate zinc sulphate, 1-3 parts and zeolite powder 10-20 parts of borax；
Wherein, the viable count of streptomyces fradiae CLIMAX-VSF and bacillus licheniformis CLIMAX-VBL be respectively 5,000,000,000/gram with
Upper and 200,000,000/gram or more.
2. microbial inoculum according to claim 1, which is characterized in that the microbial inoculum contains the component of following parts by weight: Freund chain
8 parts of mould CLIMAX-VSF microbial inoculum, 8 parts of bacillus licheniformis CLIMAX-VBL microbial inoculum, 15 parts of potassium fulvate, humic acid miberal powder 30
Part, 15 parts of leached tea oil slag, 8 parts of cigarette stalk powder, 5 parts of powdery sodium silicate, 3 parts of monohydrate zinc sulphate, 2 parts and 15 parts of zeolite powder of borax.
3. the preparation method of microbial inoculum as claimed in claim 1 or 2, which is characterized in that prepare streptomyces fradiae CLIMAX-VSF respectively
Microbial inoculum and bacillus licheniformis CLIMAX-VBL microbial inoculum, then mixed in proportion with other ingredients to get.
4. according to the method described in claim 3, it is characterized in that, the streptomyces fradiae microbial inoculum is made as follows
It is standby:
S11, streptomyces fradiae CLIMAX-VSF seed liquor is prepared；
The solid multiplication culture of S12, streptomyces fradiae CLIMAX-VSF: the seed liquor of step S11 is linked into solid multiplication culture
In base, cultivated under 30 ± 2 DEG C of sterile aerobic conditions 7-10 days to viable count be 5,000,000,000/gram or more；The solid multiplication
Culture medium contain 20-40 parts of 50-70 parts of wheat bran, 3-6 parts of brown sugar, cottonseed cake or soya-bean cake, 1-3 parts of magnesium sulfate, 1-3 parts of sodium chloride,
1-4 parts of calcium carbonate, pH7.0-7.5；
S13, the culture medium after Multiplying culture is dried to get streptomyces fradiae CLIMAX-VSF microbial inoculum.
5. the method according to claim 3 or 4, which is characterized in that the bacillus licheniformis CLIMAX-VBL microbial inoculum is pressed
As prepared by under type:
S21, the bacillus licheniformis CLIMAX-VBL being stored in freeze-drying pipe is linked into the solid slope of broth bouillon,
It is cultivated 24-48 hours at 30-32 DEG C；
In S22, picking colony access PDA solid slope, cultivated 3-7 days at 30-32 DEG C；
S23, picking colony are linked into the fluid nutrient medium by sterilizing, are cultivated under the conditions of 30-32 DEG C, 150-200r/min
36-48 hours, obtain the fermentation liquid of bacillus licheniformis CLIMAX-VBL；
Wherein, the fluid nutrient medium contains the component of following parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, sulphur
5 parts of sour magnesium, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid is mixed with solid absorbent by the weight ratio of 1:4-8, carried out after dry viable count detection to get；
Wherein, the solid absorbent is at least one of turf, zeolite or calcium carbonate.
6. containing the biological organic bacterial manure of microbial inoculum as claimed in claim 1 or 2.
7. application of the microbial inoculum as claimed in claim 1 or 2 in rehabilitating soil vigor and in increasing soil fertility.
8. application of the microbial inoculum as claimed in claim 1 or 2 in preparation biological organic bacterial manure.
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| CN109749961B (en) * | 2019-02-20 | 2022-10-04 | 金正大生态工程集团股份有限公司 | Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof |
| CN111699776A (en) * | 2020-06-18 | 2020-09-25 | 南京中泰平祥化工科技有限公司 | Construction method for recovering vegetation by improving acid heavy metal mine in-situ matrix |
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|---|---|---|---|---|
| CN1990432A (en) * | 2005-12-28 | 2007-07-04 | 郭宗恒 | Processing method for humic acidic ammonium complex blending fertilizer |
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN102876576A (en) * | 2012-09-24 | 2013-01-16 | 湖南泰谷生物科技有限责任公司 | Acidic soil conditioner, and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990432A (en) * | 2005-12-28 | 2007-07-04 | 郭宗恒 | Processing method for humic acidic ammonium complex blending fertilizer |
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN102876576A (en) * | 2012-09-24 | 2013-01-16 | 湖南泰谷生物科技有限责任公司 | Acidic soil conditioner, and preparation method and application thereof |
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