CN106399160A - Acid soil activity remediation inoculant and preparation method thereof - Google Patents
Acid soil activity remediation inoculant and preparation method thereof Download PDFInfo
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- CN106399160A CN106399160A CN201610799727.XA CN201610799727A CN106399160A CN 106399160 A CN106399160 A CN 106399160A CN 201610799727 A CN201610799727 A CN 201610799727A CN 106399160 A CN106399160 A CN 106399160A
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- bacillus licheniformis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention provides an acid soil activity remediation inoculant. The inoculant takes streptomyces fradiae and bacillus licheniformis as active ingredients. Preferably, the streptomyces fradiae CLIMAX-VSF with preservation number as CGMCC No.5133 and the bacillus licheniformis CLIMAX-VBL with preservation number as CGMCC No.12499 are taken as the active ingredients. The invention also provides a preparation method of the inoculant. The product (the inoculant), which consists of high-activity microorganisms, alkaline raw materials (such as sodium silicate and potassium fulvic acid) and high-quality organic matters, can effectively improve and condition acid soil and significantly improve micro-ecological environment of crop rhizosphere soil, so that the microorganisms can be rapidly planted and a dominant flora can be formed; therefore, favorable conditions are created for offering nutrient ingredients to the growth of plants.
Description
Technical field
The present invention relates to agricultural microorganism preparation and soil fertilizer field, specifically, it is related to a kind of acid ground vigor
Remediation microbial inoculum and preparation method thereof.
Background technology
Agricultural production needs soil to provide nutrient substance and the living environment of plant growth.Soil is as a complicated life
State system, for many years because irrational fertilizer and pesticide is using so that soil acidification, hardened, micro organism quantity declines, organic
Content declines, soil-borne disease is increasingly serious etc., thus having a negative impact to the healthy growth of crops.Research finds, passes through
The microorganism species of input soil and its nutritional labeling are carried out reasonably combined, pH value is adjusted to 8-9, root of the crop will be obviously improved
Border soil microenvironment, notable rehabilitating soil vigor, lifting crop yield and quality.
Content of the invention
It is an object of the invention to provide a kind of new acid ground vigor remediation microbial inoculum and preparation method thereof.
In order to realize the object of the invention, the present invention provides a kind of acid ground vigor remediation microbial inoculum, and its effective ingredient is not
Family name streptomycete (Streptomyces Fradiae) and Bacillus licheniformis (Bacillus Licheniformis).Preferably,
The effective ingredient of described remediation microbial inoculum is the streptomyces fradiae (Streptomyces of CGMCC No.5133 for preserving number
Fradiae) CLIMAX-VSF and preserving number are the Bacillus licheniformis (Bacillus of CGMCC No.12499
Licheniformis)CLIMAX-VBL.
Wherein, streptomyces fradiae CLIMAX-VSF is to be isolated from the soil of high-yield field, and this bacterial strain very significantly resists
Disease, fixing nitrogen, dissolving phosphor and dissolving potassium and secretion auxin performance.In China Committee for Culture Collection of Microorganisms's common micro-organismss
The heart carries out preservation, and preservation accession designation number is CGMCC No.5133, and preservation date is August in 2011 12.
Bacillus licheniformis (Bacillus Licheniformis) CLIMAX-VBL is to divide from crop root part soil
From, purification, rejuvenation and obtain, this bacterial strain can significantly improve crop yield and anti-adversity ability, significantly reduces crop pest.Bacterial strain
CLIMAX-VBL has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address Beijing is exposed to the sun
Area North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.12499,
Preservation date on May 25th, 2016.
The acid ground vigor remediation microbial inoculum that the present invention provides, the component containing following weight portion：Streptomyces fradiae microbial inoculum
5-10 part, bacillus licheniformis agent 5-10 part, potassium fulvate 10-20 part, humic acid breeze 20-40 part, leached tea oil slag 10-20
Part, cigarette stalk powder 5-10 part, powdery sodium silicate 3-6 part, zinc sulfate monohydrate 2-5 part, Borax 1-3 part and zeolite powder 10-20 part.
Preferably, in microbial inoculum contain 8 parts of streptomyces fradiae microbial inoculum, 8 parts of bacillus licheniformis agent, 15 parts of potassium fulvate,
30 parts of humic acid breeze, 15 parts of leached tea oil slag, 8 parts of cigarette stalk powder, 5 parts of powdery sodium silicate, 3 parts of zinc sulfate monohydrate, 2 parts of Borax and zeolite
15 parts of powder.
In the acid ground vigor remediation microbial inoculum that the present invention provides, the viable count of streptomyces fradiae and Bacillus licheniformis divides
Not Wei 5,000,000,000/gram more than and 200,000,000/gram more than.
The acid ground vigor remediation microbial inoculum that the present invention provides, can be prepared as follows：Prepare Freund chain respectively
Mould bacteria agent and bacillus licheniformis agent, are then mixed with other dispensings in proportion, obtain final product.
Described streptomyces fradiae microbial inoculum can be prepared as follows：
S11, prepare streptomyces fradiae seed liquor；
S12, the solid multiplication culture of streptomyces fradiae：The seed liquor of step S11 is linked in solid multiplication culture medium,
Under 30 ± 2 DEG C of aseptic aerobic conditions culture 7-10 days to viable count be 5,000,000,000/gram more than；Described solid multiplication culture medium contains
There are Testa Tritici 50-70 part, brown sugar 3-6 part, cottonseed cake or soybean cake 20-40 part, magnesium sulfate 1-3 part, sodium chloride 1-3 part, Calcium Carbonate 1-4
Part, pH7.0-7.5；
S13, the culture medium after enrichment culture is dried, obtains final product streptomyces fradiae microbial inoculum.
Described bacillus licheniformis agent can be prepared as follows：
S21, the Bacillus licheniformis being stored in lyophilizing pipe are linked in the solid slope of broth bouillon, in 30-
24-48 hour is cultivated at 32 DEG C；
S22, picking colony access in PDA solid slope, cultivate 3-7 days at 30-32 DEG C；
S23, picking colony are linked in the fluid medium of sterilizing, under the conditions of 30-32 DEG C, 150-200r/min
Culture 36-48 hour, obtains the fermentation liquid of Bacillus licheniformis；
Wherein, described fluid medium contains the component of following weight portion：20 parts of sucrose, 5 parts of sodium chloride, peptone 20
Part, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid and solid absorbent are pressed 1:The weight of 4-8, than mixing, carries out viable count detection, that is, after being dried
?.
Wherein, described solid absorbent is at least one in turf, zeolite or Calcium Carbonate etc..
Make powdery microbial inoculum product by the above process, soil can be ploughed under with site preparation, or apply or pouring root administration in Stage of Top Dressing punching.
The present invention also provides the biological organic bacterial manure containing described acid ground vigor remediation microbial inoculum.
The present invention also provides application in preparation biological organic bacterial manure for the described acid ground vigor remediation microbial inoculum.
The present invention further provides described acid ground vigor remediation microbial inoculum in rehabilitating soil vigor and improves soil fertilizer
Application in power.
Streptomyces fradiae CLIMAX-VSF produces Multiple Classes of Antibiotics, through many years of field application, to bacillary, fungoids even
It is that Viral plant disease has good prevention and control effect.In addition, this bacterial strain has outstanding fixed nitrogen, phosphorus decomposing, potassium decomposing
Can, the various plants auxin including GA3, IAA, CTK can be secreted, to lifting crop alimentary absorption level, there is obvious work
With；Bacillus licheniformis CLIMAX-VBL can be to compositions such as exocytosiss antifungal peptide, chitinases, to various plants soil-borne disease
Evil (particularly fungal disease) has good prevention and control to act on.Both the above strain is through a large amount of combine experiments it was demonstrated that compound make
The aseptic middle antagonism of agent, and the holding time reach commercial requirements, properties of product obtain in terms of anti-soil-borne disease further plus
By force.On the basis of streptomyces fradiae CLIMAX-VSF, by the addition of Bacillus licheniformis CLIMAX-VBL, lifting further is made
Thing resists soil-borne disease performance, and the stability of preparation further enhances (bacterial strain CLIMAX-VBL is more resistant to high temperature), by Freund chain
The composite bacteria agent capable of mycete CLIMAX-VSF and Bacillus licheniformis CLIMAX-VBL preparation has culture fertility, improves chemical fertilizer
Utilization rate, the suppression absorption to nitrate nitrogen, heavy metal and pesticide for the crop, have significant purification and rehabilitating soil effect.
The present invention has advantages below：
(1) this product is made up of high-activity microorganism, basic materials (as sodium silicate and potassium fulvate) and high-quality Organic substance,
Can be effectively improved and nurse one's health acid ground, notable Crop Improvement rhizosphere soil microecological environment, enable microorganism rapid field planting simultaneously
Form dominant microflora, provide nutritional labeling for plant growing, create favorable conditions.
(2) two kinds of effective ingredient -- streptomyces fradiae CLIMAX-VSF and Bacillus licheniformis CLIMAX-VBL is not mutually short of money
Anti-, the two compounding use, there is significant synergism.
Specific embodiment
Following examples are used for the present invention is described, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional meanses that are well known to those skilled in the art of technological means used, raw materials used be commercial goods.
Unless otherwise noted, heretofore described " part " refers to " weight portion ".
The streptomyces fradiae microbial inoculum being related in following examples can be prepared as follows：
S11, prepare streptomyces fradiae CLIMAX-VSF seed liquor；
S12, the solid multiplication culture of streptomyces fradiae：The seed liquor of step S11 is linked in solid multiplication culture medium,
Under 30 ± 2 DEG C of aseptic aerobic conditions culture 7-10 days to viable count be 5,000,000,000/gram more than；Described solid multiplication culture medium contains
There are Testa Tritici 50-70 part, brown sugar 3-6 part, cottonseed cake or soybean cake 20-40 part, magnesium sulfate 1-3 part, sodium chloride 1-3 part, Calcium Carbonate 1-4
Part, pH7.0-7.5；
S13, the culture medium after enrichment culture is dried, obtains final product streptomyces fradiae CLIMAX-VSF microbial inoculum.
The bacillus licheniformis agent being related in following examples can be prepared as follows：
S21, the solid that the Bacillus licheniformis CLIMAX-VBL being stored in lyophilizing pipe is linked into broth bouillon are oblique
In face, cultivate 24-48 hour at 30-32 DEG C；
S22, picking colony access in PDA solid slope, cultivate 3-7 days at 30-32 DEG C；
S23, picking colony are linked in the fluid medium of sterilizing, under the conditions of 30-32 DEG C, 150-200r/min
Culture 36-48 hour, obtains the fermentation liquid of Bacillus licheniformis；
Wherein, described fluid medium contains the component of following weight portion：20 parts of sucrose, 5 parts of sodium chloride, peptone 20
Part, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid and solid absorbent are pressed 1：6 weight, than mixing, carries out viable count detection after being dried, obtains final product ground
Clothing bacillus cereuss CLIMAX-VBL microbial inoculum.
Wherein, described solid absorbent is at least one in turf and/or zeolite or Calcium Carbonate.
Embodiment 1 acid ground vigor remediation microbial inoculum and preparation method thereof
8 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum is contained in the acid ground vigor remediation microbial inoculum that the present embodiment provides,
8 parts of Bacillus licheniformis CLIMAX-VBL microbial inoculum, 15 parts of potassium fulvate, 30 parts of humic acid breeze, 15 parts of leached tea oil slag, cigarette stalk powder 8
Part, 5 parts of powdery sodium silicate, 3 parts of zinc sulfate monohydrate, 2 parts of Borax and 15 parts of zeolite powder.Wherein, streptomyces fradiae and lichens spore
The viable count of bacillus is respectively 5,000,000,000/gram above with 200,000,000/gram more than.
Remediation microbial inoculum in embodiment 1-4 can be prepared as follows：Prepare streptomyces fradiae microbial inoculum and ground respectively
Clothing gemma bacillus agent, is then mixed with other dispensings in proportion, obtains final product.
Embodiment 2 acid ground vigor remediation microbial inoculum and preparation method thereof
5 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum is contained in the acid ground vigor remediation microbial inoculum that the present embodiment provides,
5 parts of Bacillus licheniformis CLIMAX-VBL microbial inoculum, 10 parts of potassium fulvate, 20 parts of humic acid breeze, 10 parts of leached tea oil slag, cigarette stalk powder 5
Part, 3 parts of powdery sodium silicate, 2 parts of zinc sulfate monohydrate, 1 part of Borax and 10 parts of zeolite powder.Wherein, streptomyces fradiae and lichens spore
The viable count of bacillus is respectively 5,000,000,000/gram above with 200,000,000/gram more than.
Embodiment 3 acid ground vigor remediation microbial inoculum and preparation method thereof
10 parts of streptomyces fradiae CLIMAX-VSF microbial inoculum is contained in the acid ground vigor remediation microbial inoculum that the present embodiment provides,
10 parts of Bacillus licheniformis CLIMAX-VBL microbial inoculum, 20 parts of potassium fulvate, 40 parts of humic acid breeze, 20 parts of leached tea oil slag, cigarette stalk powder
10 parts, 6 parts of powdery sodium silicate, 5 parts of zinc sulfate monohydrate, 3 parts of Borax and 20 parts of zeolite powder.Wherein, streptomyces fradiae and lichens bud
The viable count of spore bacillus is respectively 5,000,000,000/gram above with 200,000,000/gram more than.
The application of embodiment 4 acid ground vigor remediation microbial inoculum
Streptomyces fradiae CLIMAX-VSF and Bacillus licheniformis are contained in the acid ground vigor remediation microbial inoculum of the present invention
CLIMAX-VBL, through field test checking, has very good using effect.
Wherein, streptomyces fradiae CLIMAX-VSF is that a kind of safety is very high, can be used for biological husbantry and bio-pharmaceuticals
Strain, it is secreted the various plants auxin such as GA3, IAA, CTK, and possesses significant controlling disease performance, can prevent and treat including
Damping-off, verticillium wilt, melasma, downy mildew, root rot, viral mosaic disease etc. more than 30 plant Major Diseases, and this bacterium is simultaneously to line
Worm has inhibitory action, has good volume increase and Biological control performance.
Bacillus licheniformis CLIMAX-VBL is nontoxic to people and animals, fast growth, and nutritional need is simple, heat-resisting and anti-
Inverse property is strong, and it can be to compositions such as exocytosiss antifungal peptide, chitinases, to various plants soil-borne disease (particularly fungoids
Disease) there are good prevention and control to act on.This bacterium strong adaptability, stable performance, there is good promotion plant growth, culture fertility,
Improve chemical fertilizer utilization rate, the suppression absorption to nitrate nitrogen, heavy metal and pesticide for the crop, there is significant purification and reparation soil
Earth acts on.
Both the above microbial bacteria belongs to the safe biologic bacterium that biohazard degree is four classes and (under normal circumstances will not
Cause the microorganism of the mankind or Animal diseases), this two kinds of bacterium are analyzed through laboratory research, each other no Antagonistic reaction, can have
Effect is improved and conditioning acid ground, notable Crop Improvement rhizosphere soil microecological environment, enables microorganism rapid field planting and is formed
Dominant microflora, provides nutritional labeling for plant growing, creates favorable conditions.
Field test comparison is as follows：
1st, Fructus Cucumidis sativi：Every mu of acid ground vigor remediation microbial inoculum 40kg using embodiment 1 spreads manuer in holes, output increased 19.2-
26.7%, cucumber fusarium axysporum does not have the plot using to mitigate 35.2-47.6% than comparison, ripe 5-7 days ahead of time.Dramatically increase kind
Plant benefit.
2nd, Capsicum annuum L.：Every mu of acid ground vigor remediation microbial inoculum 40kg using embodiment 1 spreads manuer in holes, output increased 16.1-
24.3%, Pepper Phytophthora Blight does not have the plot using to mitigate 44.5-59.4% than comparison, ripe 3-5 days ahead of time.
3rd, Fructus Vitis viniferae：Every mu of acid ground vigor remediation microbial inoculum 80kg ditch spread using embodiment 1, output increased 20.9-
30.4%, ripe 5-7 days ahead of time, the bright-coloured gloss of fruit color, sugariness increases by more than 2 degree, storage endurance.
Embodiment 2 has similar effect with 3 acid ground vigor remediation microbial inoculum.
Although, above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. a kind of acid ground vigor remediation microbial inoculum is it is characterised in that effective ingredient is streptomyces fradiae (Streptomyces
) and Bacillus licheniformis (Bacillus Licheniformis) Fradiae.
2. microbial inoculum according to claim 1 it is characterised in that effective ingredient for preserving number be CGMCC No.5133 not
Family name streptomycete (Streptomyces Fradiae) CLIMAX-VSF and preserving number are the lichens spore bar of CGMCC No.12499
Bacterium (Bacillus Licheniformis) CLIMAX-VBL.
3. microbial inoculum according to claim 1 and 2 is it is characterised in that contain the component of following weight portion：Streptomyces fradiae bacterium
Agent 5-10 part, bacillus licheniformis agent 5-10 part, potassium fulvate 10-20 part, humic acid breeze 20-40 part, leached tea oil slag 10-20
Part, cigarette stalk powder 5-10 part, powdery sodium silicate 3-6 part, zinc sulfate monohydrate 2-5 part, Borax 1-3 part and zeolite powder 10-20 part；
Preferably, described microbial inoculum contains the component of following weight portion：8 parts of streptomyces fradiae microbial inoculum, bacillus licheniformis agent 8
Part, 15 parts of potassium fulvate, 30 parts of humic acid breeze, 15 parts of leached tea oil slag, 8 parts of cigarette stalk powder, 5 parts of powdery sodium silicate, zinc sulfate monohydrate 3
Part, 2 parts of Borax and 15 parts of zeolite powder.
4. the microbial inoculum according to any one of claim 1-3 is it is characterised in that wherein streptomyces fradiae and Bacillus licheniformis
Viable count be respectively 5,000,000,000/gram above with 200,000,000/gram more than.
5. microbial inoculum described in any one of claim 1-4 preparation method it is characterised in that prepare respectively streptomyces fradiae microbial inoculum and
Bacillus licheniformis agent, is then mixed with other dispensings in proportion, obtains final product.
6. method according to claim 5 is it is characterised in that described streptomyces fradiae microbial inoculum is made as follows
Standby：
S11, prepare streptomyces fradiae seed liquor；
S12, the solid multiplication culture of streptomyces fradiae：The seed liquor of step S11 is linked in solid multiplication culture medium, 30 ±
Under 2 DEG C of aseptic aerobic conditions culture 7-10 days to viable count be 5,000,000,000/gram more than；Described solid multiplication culture medium contains wheat
Bran 50-70 part, brown sugar 3-6 part, cottonseed cake or soybean cake 20-40 part, magnesium sulfate 1-3 part, sodium chloride 1-3 part, Calcium Carbonate 1-4 part,
pH7.0-7.5；
S13, the culture medium after enrichment culture is dried, obtains final product streptomyces fradiae microbial inoculum.
7. the method according to claim 5 or 6 is it is characterised in that described bacillus licheniformis agent enters as follows
Row preparation：
S21, the Bacillus licheniformis being stored in lyophilizing pipe are linked in the solid slope of broth bouillon, at 30-32 DEG C
Lower culture 24-48 hour；
S22, picking colony access in PDA solid slope, cultivate 3-7 days at 30-32 DEG C；
S23, picking colony are linked in the fluid medium of sterilizing, cultivate under the conditions of 30-32 DEG C, 150-200r/min
36-48 hour, obtains the fermentation liquid of Bacillus licheniformis；
Wherein, described fluid medium contains the component of following weight portion：20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, sulfur
5 parts of sour magnesium, 3 parts of potassium dihydrogen phosphate and 1000 parts of water；
S24, fermentation liquid and solid absorbent are pressed 1:The weight of 4-8, than mixing, carries out viable count detection after being dried, obtains final product；
Wherein, described solid absorbent is at least one in turf, zeolite or Calcium Carbonate.
8. the biological organic bacterial manure containing microbial inoculum described in any one of claim 1-4.
9. application in rehabilitating soil vigor and in increasing soil fertility for the microbial inoculum described in any one of claim 1-4.
10. application in preparation biological organic bacterial manure for the microbial inoculum described in any one of claim 1-4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107417471A (en) * | 2017-09-06 | 2017-12-01 | 汪建利 | Fertilizer saves half, the soil fertility activator and preparation method thereof of the crop not underproduction |
| CN109749961A (en) * | 2019-02-20 | 2019-05-14 | 金正大生态工程集团股份有限公司 | A kind of microbial bacterial agent and the preparation method and application thereof for repairing garlic continuous cropping soil |
| CN111699776A (en) * | 2020-06-18 | 2020-09-25 | 南京中泰平祥化工科技有限公司 | Construction method for recovering vegetation by improving acid heavy metal mine in-situ matrix |
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| CN1990432A (en) * | 2005-12-28 | 2007-07-04 | 郭宗恒 | Processing method for humic acidic ammonium complex blending fertilizer |
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN102876576A (en) * | 2012-09-24 | 2013-01-16 | 湖南泰谷生物科技有限责任公司 | Acidic soil conditioner, and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1990432A (en) * | 2005-12-28 | 2007-07-04 | 郭宗恒 | Processing method for humic acidic ammonium complex blending fertilizer |
| CN102329754A (en) * | 2011-09-29 | 2012-01-25 | 北京可力美施生物科技有限公司 | Novel strain of streptomyces fradiae and application thereof |
| CN102876576A (en) * | 2012-09-24 | 2013-01-16 | 湖南泰谷生物科技有限责任公司 | Acidic soil conditioner, and preparation method and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107417471A (en) * | 2017-09-06 | 2017-12-01 | 汪建利 | Fertilizer saves half, the soil fertility activator and preparation method thereof of the crop not underproduction |
| CN109749961A (en) * | 2019-02-20 | 2019-05-14 | 金正大生态工程集团股份有限公司 | A kind of microbial bacterial agent and the preparation method and application thereof for repairing garlic continuous cropping soil |
| CN109749961B (en) * | 2019-02-20 | 2022-10-04 | 金正大生态工程集团股份有限公司 | Microbial agent for repairing garlic continuous cropping soil and preparation method and application thereof |
| CN111699776A (en) * | 2020-06-18 | 2020-09-25 | 南京中泰平祥化工科技有限公司 | Construction method for recovering vegetation by improving acid heavy metal mine in-situ matrix |
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| CN106399160B (en) | 2019-08-16 |
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