CN103435395A - Drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and preparation method thereof - Google Patents

Drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and preparation method thereof Download PDF

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CN103435395A
CN103435395A CN 201310157959 CN201310157959A CN103435395A CN 103435395 A CN103435395 A CN 103435395A CN 201310157959 CN201310157959 CN 201310157959 CN 201310157959 A CN201310157959 A CN 201310157959A CN 103435395 A CN103435395 A CN 103435395A
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drip
fertilizer
biological fertilizer
preparation method
salt solution
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CN 201310157959
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武占省
彭艳杰
郭丽娜
鲁建江
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石河子大学
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Abstract

The invention relates to a drip-irrigation type salt stress-alleviating and disease-preventing biological bacterial fertilizer and a preparation method thereof. The drip-irrigation type salt stress-relieving and disease-preventing biological bacterial fertilizer comprises nutrition carriers, additives, microelements and functional microorganisms, wherein the nutrition carriers comprise two or more of humic acid, glutamic acid, urea, corn flour, potassium sulphate, potassium chloride and diammonium hydrogen phosphate; the additives comprise two or more of bentonite, sodium carboxymethyl cellulose, sodium alginate an polyvinyl alcohol; the microelements comprise one or two of boric acid an ammonium molybdate; and the functional microorganisms is formed by combining salt stress-alleviating growth-promoting bacteria and disease-preventing growth-promoting bacteria, wherein the salt stress-alleviating growth-promoting bacteria is one or two selected from pseudomonas putida and azotobacter chroococcum; and the disease-preventing growth-promoting bacteria is one or two selected from bacillus subtilis and bacillus megatherium. The biological bacterial fertilizer is in a liquid dosage form, has relatively high effective viable count and relatively long quality guarantee period. The effective viable count of the biological bacterial fertilizer is higher than or equal to 10<10> cfu/mL; and total nutrient content of (N+P2O5+K2O) is higher than or equal to 12%.

Description

一种滴灌剂型解盐防病生物菌肥及其制备方法 Solutions of salts of one kind of drip preventing and biological fertilizer formulation and preparation method

技术领域 FIELD

[0001] 本发明涉及的是微生物领域,具体的说是一种滴灌剂型解盐防病生物菌肥及其制备方法。 [0001] The present invention relates to the field of microbiology, specifically a prophylactic Salt Solution Drip biological fertilizer formulation and preparation method.

背景技术 Background technique

[0002] 新疆地处我国西北内陆,由于强烈的地面蒸发和作物蒸腾促使地下水中的盐分向上运移,从而引起土壤中盐分的累积;同时,不适当的农业灌溉和水利设施,引起土壤盐溃化,从而影响农业种植。 [0002] Xinjiang is located in the Inland Northwest China, due to the strong surface evaporation and plant transpiration prompted salinity groundwater migrated upward, causing the accumulation of salts in the soil; at the same time, inappropriate irrigation and water conservancy facilities, causing soil salt collapse, thereby affecting farming. 新疆其中近80个县(市)都面临严重的土壤盐溃化问题,以新疆南部地区尤为突出,如喀什地区的盐溃化耕地约占到地区耕地面积的48.72%。 Xinjiang, of which nearly 80 counties (cities) are facing serious problems of soil salinization, especially to southern Xinjiang, such as salt Kashgar collapse of arable land accounts for about 48.72% of the arable land area. 近年来,各种类型的次生盐碱化日益严重,造成作物缺苗、断垄、产量下降,严重制约了新疆农业生产的发展。 In recent years, various types of secondary salinization growing, resulting in crop Deficiency, broken ridge, production fell, seriously hampered the development of agricultural production in Xinjiang. 因此,需要从新的角度研究土壤盐溃化的解决措施,其中利用生物技术改良盐碱地,缓解植物盐胁迫已日益受到重视。 Therefore, the need for a new perspective to study and solve measure soil salinization, where the use of biotechnology improve saline, salt stress relieve the plant has received increasing attention.

[0003] 另外,由于新疆早春低温、温度变化幅度大,常引起苗期立枯病普遍发生,为害严重。 [0003] Further, since the low Xinjiang in early spring, the temperature variation amplitude, often caused by Rhizoctonia widespread, serious damage. 由立枯病病原Rhizictonia solani Kuhn引起的棉苗烂根病一般发病率为27%-75%,严重的达90%以上,造成缺苗断垄,大大影响作物产量和质量。 The pathogen Rhizoctonia solani Kuhn induced Cotton Rhizictonia rot incidence of disease is generally 27% to 75%, more than 90% of severely, causing ridge sprout deficiency, greatly affect the crop yield and quality. 化学防治是主要的防治措施,但存在污染环境,易造成农药残留,易使病原菌产生抗药性,破坏生态平衡等问题,通常难以达到满意的防治效果。 Chemical control is the main control measures, but there is pollution of the environment, could easily lead to pesticide residues, easy to make pathogens resistant, destroy the ecological balance and other issues, it is often difficult to achieve satisfactory control effect. 因此,需要寻找新途径对病害防治进行研究,其中生物防治在防治土传病害方面已成为研究热点。 Therefore, the need to find new ways to study disease control, biological control which has become a hot topic in the fight against soil-borne diseases.

[0004] 近年来研究表明,根际有益微生物可以促进作物的生长发育,提高产量,更可帮助作物耐受一些盐碱、干旱、重金属等逆境条件。 [0004] In recent years, studies have shown that rhizosphere beneficial microorganisms can promote the growth of crops, increase production, but also help crops withstand some of salinity, drought, heavy metals and other adverse conditions. 因此,利用根围促生菌帮助作物提高对土壤盐溃化耐受性,开发具有良好促生作用的微生物制剂已日益受到关注。 Thus, with the help rhizosphere PGPR increase plant resistance to crushing of soil salts, microbial preparation having good development promoting effect has been a growing concern. 此外,在植物根际存在着许多对作物病害有良好控制效果的根际有益微生物,而且可以通过直接和间接两方面的作用对植物病害进行控制,同时增加植株抗逆性和生长势。 In addition, there are many beneficial microorganisms in the rhizosphere have a good control effect on crop diseases in the plant rhizosphere, and plant diseases can be controlled by both the indirect and direct effects, while increasing the plant resistance and vigor. 因此,采用生物方法解决这些问题是目前的发展趋势。 Therefore, the use of biological methods to solve these problems is the current trend.

[0005] 然而,目前对于微生物肥料功能也比较单一,以及菌株与营养载体系统的筛选研究也较缺乏。 [0005] However, the microbial fertilizer function is relatively simple, as well as screening and nutrition research strains carrier systems are more scarce. 此外,对于滴灌模式应用的微生物肥料的研究目前仍属空白,且国外也尚未有同类竞争性产品。 In addition, the study of microbial fertilizer for drip irrigation mode applications this is still blank, and also abroad have not yet competing products. 本发明一种滴灌剂型的解盐防病生物菌肥,适应于新疆气候环境和适合于滴灌模式下应用的解盐防病功能微生物肥料产品,通过滴灌施入方式将菌肥施入到植物根际,使活菌体更容易地定殖于植物根际,促进菌体与作物的互作效应,有效提高菌体在作物根际定殖率、存活量和持效性,充分发挥菌体的效能,解决了以往微生物肥料普遍存在的持效性差、稳定性低、应用效果不明显等问题。 One kind of drip dosage form salts of the present invention prevent disease biological fertilizer solutions, adapted to the climate and Xinjiang salt solution suitable for use in disease prevention function drip mode microbial fertilizer products, applied by drip irrigation fertilizer was applied to the embodiment to the plant roots occasion, make it easier to live bacteria to colonize the rhizosphere, to promote interaction with the effector cells of crops, improve the rhizosphere bacterial colonization, survival, and the amount of residual effect, full of bacteria performance, solves the microbial fertilizer difference prevailing holding effect, low stability, the effect is not obvious application problems. 因此,本发明专利开发的适于新疆滴灌模式的生物菌肥及制备方法,必将具有更大的应用潜力和广阔的市场前景。 Thus, biological fertilizer and preparation methods suitable Drip Irrigation development mode of the present invention patent, will have a greater application potential and broad market prospect.

发明内容 SUMMARY

[0006] 本发明的目的在于提供一种有效活菌数高、成本低、适用于滴灌种植模式应用的,对植物具有缓解盐胁迫、防治病害及促进生长等功效的生物菌肥及其制备方法。 [0006] The object of the present invention is to provide a highly effective number of viable, low cost, pattern suitable for drip irrigation applications, relieving plant salt stress, disease prevention and biological fertilizer and preparation method of promoting the growth and other effects . [0007] 本发明的目的是通过下述技术方案实现的。 Objective [0007] The present invention is achieved by the following technical solutions.

[0008] 本发明的一种滴灌型解盐防病生物菌肥及其制备方法,它包含营养载体、助剂、微量元素和功能微生物;其中,生物菌肥中有效菌含量≥ 1010cfu/ml,总养分(Ν+Ρ205+Κ20)≥12%,且保存半年本发明的菌肥中有效活菌含量≥108cfu/ml。 [0008] A drip type biological fertilizer prophylactic salt solution and a preparation method of the present invention, which comprises nutrition carriers, adjuvants, trace elements and functional microorganism; wherein the biological fertilizer effective bacterial content ≥ 1010cfu / ml, total nutrient (Ν + Ρ205 + Κ20) ≥12%, and effective fertilizer saving half of viable cells of the present invention ≥108cfu / ml.

[0009] 所述的一种滴灌剂型解盐防病生物菌肥,其特征在于包括:以肥料总体质量为100% 计: [0009] A drip dosage of the prophylactic biological fertilizer salt solution, characterized by comprising: overall quality fertilizer as 100%:

[0010]营养载体 10% -40% ; [0010] 10% -40% nutrient carrier;

[0011]助剂 5%-15%; [0011] 5% -15% additives;

[0012] 微量元素 1%-2%; [0012] Trace Elements 1% -2%;

[0013] 微生物发酵液 50%-80%。 [0013] microbial broth 50% -80%.

[0014] 所述营养载体包含腐殖酸、谷氨酸、尿素、玉米粉、硫酸钾、氯化钾、磷酸氢二铵中的两种或几种;助剂包含膨润土、羧甲基纤维素钠、海藻酸钠、聚乙烯醇中的两种或几种;微量元素包含硼酸、钥酸铵中的一种或两种。 [0014] The carrier comprises two or more nutritional humic acid, glutamic acid, urea, corn flour, potassium sulfate, potassium chloride, diammonium phosphate in; aid comprises bentonite, carboxymethyl cellulose sodium, sodium alginate, polyvinyl alcohol, two or more; trace element comprises boric acid, ammonium key in one or both.

[0015] 所述功能微生物包含解盐促生菌和防病促生菌,其中解盐促生菌包含恶臭假单胞菌(Pseudomonas putida保藏号ATCC17485)、圆褐固氮菌(Azotobacter chroococcum保藏号为ACCC10098)中的一种或两种,所述两种囷株购于中国农业微生物囷种保减管理中心。 [0015] The salt solution comprises a functional microorganism promoting bacteria and disease prevention PGPR, wherein the salt solution contains Pseudomonas putida promoting bacteria (Pseudomonas putida Accession No. ATCC17485), Azotobacter brown circle (Azotobacter chroococcum Accession No. ACCC10098) of one or both of the two lines available from China Agricultural granary granary microbial species Save security management center. 这些微生物可以降低盐胁迫下植株对Na+的绝对吸收量,增加对K+、Ca2+和Fe2+的吸收,有效地解除次生盐溃化对作物的胁迫,具有溶磷和固氮能力,有力作物对营养元素的吸收;防病促生菌包含枯草芽孢杆菌(Bacillus subtilis-NCD-2保藏号为CGMCC1019),蜡状芽孢杆菌(Bacillus cereus保藏号为CGMCC4991)中的一种或两种,所述两种菌株分别购于中国微生物菌种保藏管理委员会普通微生物中心。 These microorganisms may reduce the absolute absorption plant of the Na + salt stress, increased, Ca2 uptake of K + + and Fe2 +, and effectively relieve secondary salinization stress on the crop, having dissolved phosphorus and nitrogen fixation capability, strong crop nutrients absorption; prophylactic PGPR containing Bacillus subtilis (Bacillus subtilis-NCD-2 accession No. CGMCC1019), Bacillus cereus (Bacillus cereus accession No. CGMCC4991) of one or both of the two strains They were purchased in China Culture Collection Committee general Microbiology Center. 这些微生物对作物苗期病害,如根腐病、枯萎病及黄萎病害等有很好的预防、防治作用,可促进植株生长及对营养物质的吸收。 These microorganisms seedling diseases of crops, such as root rot, Fusarium and Verticillium wilt disease and the like have a good damage prevention, control, promotes plant growth and uptake of nutrients.

[0016] 本发明的一种滴灌剂型解盐防病生物菌肥及其制备方法,具体实现步骤如下: [0016] A dosage form of the present invention drip irrigation solution prophylactic biological fertilizer salts and its preparation method, the specific steps are as follows:

[0017] 步骤一,将低温保存的功能微生物活化、液体培养种子液,备用;其中包括: [0017] Step one, the activated functional microorganism cryopreservation, liquid seed liquid culture standby; which comprises:

[0018] ⑴NA培养基的配制 [0018] ⑴NA medium formulation

[0019]将NaCl (5.0g),蛋白胨(10.0g),牛肉膏(3.0g),H2O(1000ml)进行混合,配置NA培养基;若是固体NA培养基,则加入1.8%的琼脂;灭菌,备用; [0019] The NaCl (5.0g), peptone (10.0 g), beef extract (3.0g), H2O (1000ml) were mixed medium disposed NA; NA if the solid medium, 1.8% agar is added; Sterilization ,spare;

[0020] (2)菌株的活化 [0020] (2) activation of strain

[0021 ] 将低温保存的有益微生物在NA培养基上划线,28-32 °C下恒温培养2-3天;挑取单菌落,在新的NA固体培养基上划线培养,如此2-3次; [0021] The cryopreserved beneficial microorganisms on NA medium chain line, incubated at 28-32 ° C 2-3 days; single colony was picked, streaked on a solid medium NA new, so 2- 3 times;

[0022] (3)功能微生物的培养 [0022] (3) a microorganism culture function

[0023] 将活化后的功能微生物,接入到玉米粉、豆柏液体培养基(玉米粉50g,豆柏粉16g, K2HP042g, MnSO40.2g, NaC15g, H2OlOOOml)中,在28-32°C、150-220rpm 的条件下扩大培养至微生物数量达到1012cfu/ml以上,即培养至对数生长期,备用。 [0023] The activation function of the microorganism, access to corn flour, soybean Bo liquid medium (corn flour 50g, Parkinson soy flour 16g, K2HP042g, MnSO40.2g, NaC15g, H2OlOOOml), in a 28-32 ° C, culturing under 150-220rpm expand the number of microorganisms to reach 1012cfu / ml, i.e. cultured to logarithmic growth phase, alternate.

[0024] 步骤二,取组分质量百分比分别为10% -40%的营养载体,5%-15%的助剂,1% -2%的微量元素充分混合;灭菌,备用; [0024] Step two, take components mass percentage were 10% -40% of the nutritional support, 5% -15% additives, 1% -2% of trace elements mixed; sterilization standby;

[0025] 步骤三,将步骤一的菌液与步骤二的营养载体、助剂、微量元素混合均匀,其中,功能微生物的质量百分比为50% -80%,即获得一种滴灌剂型解盐防病生物菌肥。 [0025] Step three, the bacterial suspension of step a two step nutrient carrier, adjuvant, mixed trace elements, wherein the functional microorganism mass percentage is 50% -80%, i.e., to obtain an anti-drip dosage salt solution disease bio-fertilizer.

[0026] 经上述步骤制得的一种解盐防病生物菌肥,其成品指标如下:[0027] pH:6.8 -7.6 ; [0026] Following the above steps gave a solution of bio-fertilizer salts disease prevention, the finished indicators are as follows: [0027] pH: 6.8 -7.6;

[0028]总养分(Ν+Ρ205+Κ20)≥ 12% ; [0028] Total nutrient (Ν + Ρ205 + Κ20) ≥ 12%;

[0029] 菌肥中的有效活菌数大于101Qcfu/ml ; [0029] The effective number of viable cells is greater than fertilizer 101Qcfu / ml;

[0030] 室温下密封储存半年后,菌肥中的有效活菌数可大于108cfu/ml ; [0030] After six months storage at room temperature sealed, an effective number of viable cells in the fertilizer may be greater than 108cfu / ml;

[0031]杂菌率≤10% ; [0031] bacteria of ≤10%;

[0032] 固形物粒径≤0.1mm ; [0032] ≤0.1mm solid particle;

[0033] 与现有发明专利技术相比,本发明专利的技术创新与有益效果: [0033] Compared with the prior art patented inventions, patents and technical innovation of the present invention, the beneficial effects:

[0034] 1、本发明的生物菌肥具有缓解盐胁迫、防治苗期根腐病、枯萎病等病害等功效,并且能够促进种子发芽和作物生长,增加作物产量,提高作物品质,降低农业生产成本; [0034] 1, the biological fertilizer according to the present invention, salt stress relieving, against seedling root rot, blight and other diseases like effect, and can promote seed germination and plant growth, increasing crop yields, improving crop quality, reduced agricultural production cost;

[0035] 2、本发明的生物菌肥属于液体剂型,适于新疆滴灌种植模式下应用,通过滴灌方式将菌肥施入到作物根际,有效提高菌体定殖数量,充分发挥菌体功效,实现易于定殖,效果保证的技术突破和创新; [0035] 2, the present invention belongs to the biological fertilizer liquid dosage forms suitable for drip irrigation applications in Xinjiang mode, applied by drip irrigation fertilizer into the rhizosphere, effectively increase the number of bacterial colonization, bacterial effect full , easy to achieve colonization, the effect is to ensure that technological breakthroughs and innovations;

[0036] 3、本发明的生物菌肥,原创性的筛选有机营养载体和助剂载体系统技术,为菌体提供了生长所需的各种养分,确保有效活菌数高、保质期长及稳定性好。 [0036] 3, the biological fertilizer according to the present invention, the original screening organic nutrients carriers and adjuvants vector system technology, provided for the cells of nutrients required for growth, the effective number of viable cells to ensure high, stable and long shelf life good. 解决了以往生物肥料使用效果稳定性差的问题。 It solves the poor stability of bio-fertilizer use performance issues.

附图说明 BRIEF DESCRIPTION

[0037] 图1为解盐防病生物菌肥滴灌棉苗的效果图; [0037] FIG. 1 is a solution of a salt prophylactic biological fertilizer drip FIG Cotton effect;

[0038] 图2为空白对照效果图。 [0038] FIG. 2 is a placebo effect FIG.

具体实施方式 detailed description

[0039] 本发明的一种滴灌剂型解盐防病生物菌肥,以腐殖酸、谷氨酸、尿素、玉米粉、硫酸钾、氯化钾和磷酸氢二铵中的两种或几种作为营养载体,以膨润土、羧甲基纤维素钠、海藻酸钠、聚乙烯醇中的两种或几种作为助剂,以硼酸、钥酸铵中的一种或两种作为微量元素,以功能微生物解盐促生菌和生防菌中的各一种或两种菌株的发酵液,进行各成分的复配以开发解盐防病生物菌肥。 [0039] A dosage form of the present invention drip irrigation salt solution prophylactic biological fertilizer, humic acid, glutamic acid, urea, corn flour, potassium sulfate, potassium chloride, and diammonium phosphate in two or more as a nutritional carrier, bentonite, sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol as an adjuvant two or more, boric acid, ammonium key in one or both of trace elements as to Solutions of salts of functional microorganism promoting bacteria and Bacillus subtilis in the fermentation broth of one or two kinds of each strain, together with the development of multiplexed prophylactic biological fertilizer salt solutions of each component.

[0040] 为了充分说明本技术方案的特性以及实施本技术方案的方式,下面给出实施例,但本发明并不限于此。 [0040] In order to fully illustrate the technical solutions characteristic of the present embodiment and the embodiment of the present technology, embodiments given below, but the present invention is not limited thereto.

[0041] 制备实施例1 [0041] Preparation Example 1

[0042] 各成分的质量比如下: [0042] The mass of each component is as follows:

[0043] [0043]

Figure CN103435395AD00061

[0044] 将营养载体、助剂、微量元素中的各组分按照上述比例进行混合,在121°C条件下灭菌20分钟,备用。 [0044] The nutritional carriers, adjuvants, trace elements in the components in the above ratio were mixed, sterilized at 121 ° C for 20 min, the standby. 在无菌条件下,将事先培养好的功能微生物按照上述比例添加到上述溶液中,即获得液体剂型生物菌肥。 Under sterile conditions, in advance according to the above develop good functional microorganism ratio is added to the solution, i.e., the liquid dosage forms biological fertilizer obtained. 其中,ph值为6.8-7.6,总养分奸?205+1(20≥12.1%,菌肥中的有效活菌数大于101(lCfU/ml,且室温下保存半年后菌肥中的有效活菌数可达108cfu/ml,杂菌率≤ 10%。 Wherein, ph value of 6.8-7.6, the total nutrient rape? 205 + 1 (20≥12.1%, the effective number of viable cells in the fertilizer is greater than 101 (lCfU / ml, viable and effective fertilizer after six months of storage at room temperature number up to 108cfu / ml, bacteria of ≤ 10%.

[0045] 制备实施例2 [0045] Preparation Example 2

[0046] 各成分的质量比如下: [0046] The mass of each component is as follows:

[0047] [0047]

Figure CN103435395AD00062

[0048]玉米粉浓度 6% [0048] The concentration of 6% corn flour

硫酸钾 2% Potassium sulfate 2%

氯化钾 4% Potassium chloride 4%

磷酸氢二铵 3% Diammonium phosphate 3%

膨润土浓度 4% Bentonite concentration of 4%

海藻酸钠浓度 4% Concentration of 4% sodium alginate

硼酸浓度 0.2% Boric acid concentration of 0.2%

钥酸铵浓度 1.8% Key ammonium concentration of 1.8%

菌体发酵液 65% Bacterial fermentation broth 65%

[0049] 将营养载体、助剂、微量元素中的各组分按照上述比例进行混合,在121°C条件下灭菌20分钟,备用。 [0049] The nutritional carriers, adjuvants, trace elements in the components in the above ratio were mixed, sterilized at 121 ° C for 20 min, the standby. 在无菌条件下,将事先培养好的功能微生物按照上述比例添加到上述溶液中,即获得液体剂型生物菌肥。 Under sterile conditions, in advance according to the above develop good functional microorganism ratio is added to the solution, i.e., the liquid dosage forms biological fertilizer obtained. 其中,PH值为6.8-7.6,总养分Ν+Ρ205+Κ20≥13.06%,菌肥中的有效活菌数大于101(lCfU/ml,且室温下保存半年后菌肥中的有效活菌数可达108cfu/ml,杂菌率≤ 10%。 Wherein, PH value of 6.8-7.6, the total nutrient Ν + Ρ205 + Κ20≥13.06%, the effective number of viable cells in the fertilizer is greater than 101 (lCfU / ml, and six months after the effective number of viable cells in the fertilizer may be stored at room temperature of 108cfu / ml, bacteria of ≤ 10%.

[0050] 制备实施例3 [0050] Preparation Example 3

[0051] 各成分的质量比如下: [0051] The mass of each component is as follows:

[0052] [0052]

腐殖酸浓度 7% Humic acid concentration of 7%

尿素浓度 2% Urea concentration of 2%

玉米粉浓度 5% Concentration of 5% corn flour

硫酸钾 3% Potassium 3%

氯化钾 3% Potassium chloride 3%

磷酸氢二铵 4% Diammonium phosphate 4%

膨润土浓度 2% Bentonite concentration of 2%

[0053] [0053]

Figure CN103435395AD00081

[0054] 将营养载体、助剂、微量元素中的各组分按照上述比例进行混合,在121°C条件下灭菌20分钟,备用。 [0054] The nutritional carriers, adjuvants, trace elements in the components in the above ratio were mixed, sterilized at 121 ° C for 20 min, the standby. 在无菌条件下,将事先培养好的功能微生物按照上述比例添加到上述溶液中,即获得液体剂型生物菌肥。 Under sterile conditions, in advance according to the above develop good functional microorganism ratio is added to the solution, i.e., the liquid dosage forms biological fertilizer obtained. 其中,PH值为6.8〜7.6,总养分Ν+Ρ205+Κ20≤14.02%,菌肥中的有效活菌数大于1010CfU/ml,且室温下保存半年后菌肥中的有效活菌数可达108cfu/ml,杂菌率≤10%。 Wherein, PH value of 6.8~7.6, the total nutrient Ν + Ρ205 + Κ20≤14.02%, the effective number of viable cells is greater than fertilizer 1010CfU / ml, and stored at room temperature for six months after the effective number of viable cells in the fertilizer up 108cfu / ml, bacteria of ≤10%.

[0055] 制备实施例4 Example 4 [0055] Preparation of

[0056] 各成分的质量比如下: [0056] The mass of each component is as follows:

[0057] [0057]

Figure CN103435395AD00082

[0058] 将营养载体、助剂、微量元素中的各组分按照上述比例进行混合,在121°C条件下灭菌20分钟,备用。 [0058] The nutritional carriers, adjuvants, trace elements in the components in the above ratio were mixed, sterilized at 121 ° C for 20 min, the standby. 在无菌条件下,将事先培养好的功能微生物按照上述比例添加到上述溶液中,即获得液体剂型生物菌肥。 Under sterile conditions, in advance according to the above develop good functional microorganism ratio is added to the solution, i.e., the liquid dosage forms biological fertilizer obtained. 其中,PH值为6.8〜7.6,总养分Ν+Ρ205+Κ2014.98%,菌肥中的有效活菌数大于1010CfU/ml,且室温下保存半年后菌肥中的有效活菌数可达108cfu/ml,杂菌率≤10%。 Wherein, PH value of 6.8~7.6, the total nutrient Ν + Ρ205 + Κ2014.98%, the effective number of viable cells is greater than fertilizer 1010CfU / ml, and stored at room temperature for six months after the effective number of viable cells in the fertilizer up 108cfu / ml, bacteria of ≤10%.

[0059] 为了考察用本发明解盐防病生物菌肥滴灌的棉花生长情况,在大田环境条件下进行田间试验,在播种前对试验地的主要离子水平进行了测定,耕作土壤的总盐含量为 [0059] In order to examine the present invention, with the salt solution prophylactic biological fertilizer drip cotton growth, field trials under ambient field conditions to test the main ion levels were measured before sowing, the total salt content of the soil tillage for

0.3-0.7%。 0.3-0.7%.

[0060] 试验实施例1 [0060] Test Example 1

[0061] 将本发明的制备实施例3的解盐防病菌肥,按常规方式播种,将本发明稀释10-20倍随水滴灌每亩用量5-10公斤,施肥3-4次。 [0061] Solutions for preventing and fertilizer salts prepared in Example 3 of the present invention, diluted 10-20 times in a conventional manner by sowing with the present invention, the water drip 5-10 kg per acre fertilization 3-4 times. 出苗30天后,调查种子出苗率、发病率,并计算病情指数和防病效果,见表I。 30 days after emergence, seed germination rate survey, the incidence of, and the disease index and control effect is calculated, see Table I.

[0062] 表I解盐防病生物菌肥对棉花出苗及苗期病害的影响作用 [0062] TABLE I Solution influence on Cotton Seedling Emergence and disease prevention diseases biological fertilizer salts

[0063] [0063]

Figure CN103435395AD00091

[0064] 从表I中的结果可见,棉花种子在用本发明解盐防病生物菌肥滴灌处理后,出苗率和防病效果都有明显提高,而发病率降低。 [0064] From the results can be seen in Table I, the cotton seeds in the salt solution is treated with prophylactic biological fertilizer according to the present invention drip irrigation, seedling emergence and disease prevention effect has improved significantly, but reduced the incidence.

[0065] 试验实施例2 [0065] Test Example 2

[0066]为了考察解盐防病生物菌肥对棉花植物生物量(株高、鲜重、干重、单株叶面积、叶面积指数)的影响,见表2。 [0066] In order to examine the salt solution to prevent and biological fertilizer cotton plant biomass (plant height, fresh weight, dry weight, leaf area, leaf area index) Effect of Table 2.

[0067] 表2解盐防病生物菌肥对棉花生物量及产量的影响作用 [0067] Table 2 Effects of salt solution prophylactic effect biological fertilizer cotton and biomass yield

[0068] [0068]

Figure CN103435395AD00092

[0069] 从表2中的结果可见,棉花种子在用本发明解盐防病生物菌肥滴灌处理后,棉花植物生物量(株高、鲜重、干重、单株叶面积、叶面积指数)都有很大程度的提高。 [0069] apparent from the results in Table 2, after the cotton seeds treated with a drip preventing and biological fertilizer according to the present invention, salt solution, cotton plant biomass (plant height, fresh weight, dry weight, leaf area, leaf area index ) has largely improved.

[0070] 试验实施例3 [0070] Test Example 3

[0071]为了考察解盐防病生物菌肥对棉花的增产(单株结铃数、单铃重、亩株数、籽棉产量)的影响,见表3。 [0071] In order to examine the salt solution to prevent and biological fertilizer cotton yield (the number of bolls per plant, boll weight, acres of trees, seed cotton yield) effects, see Table 3.

[0072] 表3解盐防病生物菌肥对棉花增产作用 [0072] Table 3 Biological Fertilizer Solutions of salts prophylactic effect on cotton yield

[0073] [0073]

Figure CN103435395AD00093

[0074] 从表3中的结果可见,棉花种子在用本发明解盐防病生物菌肥滴灌处理后,棉花植物的单株结铃数、单铃重、亩株数、籽棉产量都明显增加。 [0074] As seen from Table 3 the results, cotton seeds after treatment with salt solutions of the present invention, disease prevention and drip biological fertilizer, the number of cotton bolls per plant of plants, boll weight, acres of trees, were significantly increased seed cotton yield.

Claims (8)

  1. 1.一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:它包含营养载体、助齐U、微量元素和功能微生物。 A drip prophylactic dosage biological fertilizer salt solution and a preparation method, which is characterized in that: it contains nutrient carriers, homogeneous U, trace elements and functional microorganism. 制备的生物菌肥具有缓解盐胁迫、防治土传病害和促进作物生长等功效,生物菌肥中有效菌含量≥101(lCfu/ml,总养分(Ν+Ρ205+Κ20)≥12%,固形物粒径(0.1mm,且保存半年后菌肥中有效活菌含量≥108cfu/ml。 Biological fertilizer produced salt stress relieving, preventing soil-borne diseases and promoting plant growth and other effects, the content of bacteria effective biological fertilizer ≥101 (lCfu / ml, total nutrient (Ν + Ρ205 + Κ20) ≥12%, solids (0.1mm, and six months after storage of viable cells effective fertilizer ≥108cfu / ml diameter.
  2. 2.根据权利要求书I所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于包括:以肥料总体质量为100%计: 营养载体 10% -40% ; 助剂 5%-15%; 微量元素 1% -2% ; 微生物发酵液 50%-80%。 2. A dosage form according to one of drip preventing and biological fertilizer salt solution and a preparation method according to claims I, characterized by comprising: overall quality fertilizer as 100%: 10% -40% nutrient carrier; adjuvant 5% -15%; trace elements 1-2%; microbial broth 50% -80%.
  3. 3.根据权利要求1、2所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:所述营养载体包含腐殖酸、谷氨酸、尿素、玉米粉、硫酸钾、氯化钾、磷酸氢二铵中的两种或几种;助剂包含膨润土、羧甲基纤维素钠、海藻酸钠、聚乙烯醇中的两种或几种;微量元素包含硼酸、钥酸铵中的一种或两种。 3. A dosage form according to one of drip preventing and biological fertilizer salt solution and a preparation method according to claim 1, wherein: the nutritional support comprising humic acid, glutamic acid, urea, corn flour, sulfuric acid of two or more, potassium chloride, diammonium phosphate in; aid comprises bentonite, sodium carboxymethylcellulose, sodium alginate, polyvinyl alcohol, two or more; trace element comprises boric acid, one or both of ammonium key.
  4. 4.根据权利要求1所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:所述功能微生物包含解盐促生菌和防病促生菌,其中解盐促生菌包含恶臭假单胞菌(Pseudomonas putida 保藏号ATCC17485)、圆褐固氮菌(Azotobacter chroococcum保藏号为ACCC10098)中的一种或者两种;防病促生菌包含枯草芽孢杆菌(Bacillussubtilis-NCD-2保藏号为CGMCC1019)、蜡状芽孢杆菌(Bacillus cereus保藏号为CGMCC4991)中的一种或两种。 A dosage form according prophylactic biological fertilizer salt solution and drip method of preparation as claimed in claim, wherein: the functional microbial solution comprises a salt promoting bacteria and disease prevention PGPR, wherein the salt solution to promote probiotic comprising Pseudomonas putida (Pseudomonas putida accession No. ATCC17485), Azotobacter brown circle (Azotobacter chroococcum accession No. ACCC10098) of one or both of; prophylactic PGPR comprising Bacillus subtilis (Bacillussubtilis-NCD- 2 accession number CGMCC1019), Bacillus cereus (Bacillus cereus accession No. CGMCC4991) of one or both.
  5. 5.根据权利要求4所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:各功能微生物发酵液的体积比为,恶臭假单胞菌:圆褐固氮菌:枯草芽孢杆菌:蜡状芽孢杆菌为I-2: O-2: I-3: O-3。 5. A dosage form according to one of drip preventing and biological fertilizer salt solution and a preparation method according to claim 4, wherein: each function of the microbial broth volume ratio of Pseudomonas putida: Azotobacter brown circle: Bacillus subtilis: Bacillus cereus for the I-2: O-2: I-3: O-3.
  6. 6.一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于按以下步骤或次序进行配置: ①将营养载体中的两种或几种加水溶解后,再与助剂中的两种或几种的水分散液混合,并加入微量元素中的一种或两种,121°C灭菌20min,备用。 ① The nutrition of two or more carrier dissolved in water, and then with the aid of: bio-fertilizer salts disease prevention and preparation method of a drip irrigation solution dosage form, characterized in that the configuration or order of the following steps mixing two or more aqueous dispersion, and adding one or both of trace elements, 121 ° C sterilization 20min, standby. ②在调节反应釜中将①中的营养载体、助剂、微量元素与功能微生物发酵液机械搅拌混合得成品。 ② ① adjusting nutritional support in the reaction kettle, adjuvants, trace elements and functional microbial broth was stirred mechanically mixing products.
  7. 7.根据权利要求1所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:解盐防病生物菌肥是一种液体剂型,生物菌肥中有效菌含量> lO'fu/ml,总养分(Ν+Ρ205+Κ20)≥12%,固形物粒径≥0.1mm,且保存半年后菌肥中有效活菌含量≥IO8Cfu/ml ο 7. A dosage form according to one drip salt solution and a preparation method prophylactic biological fertilizer according to claim 1, wherein: the salt solution is a prophylactic biological fertilizer liquid dosage forms, effective biological fertilizer bacteria content> lO'fu / ml, the total nutrient (Ν + Ρ205 + Κ20) ≥12%, solids particle ≥0.1mm, and effective fertilizer saving half of viable cells ≥IO8Cfu / ml ο
  8. 8.根据权利要求7所述的一种滴灌剂型解盐防病生物菌肥及其制备方法,其特征在于:开发的生物菌肥适用于滴灌种植模式下的棉花、加工番茄及小麦等作物,有效缓解土壤盐溃化,防治苗期土传病害、促进作物生长、增产效果显著,且滴灌应用过程无堵塞现象。 8. The dosage form as claimed in claim 7, one kind of drip preventing and biological fertilizer salt solution and a preparation method, wherein: Biological Fertilizer Development applied to cotton under drip irrigation mode, processing tomato and wheat and other crops, effectively alleviate soil salinization, soil-borne diseases control seedling, promote crop growth, yield significant results, and drip irrigation application process without clogging.
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CN104671997A (en) * 2015-01-30 2015-06-03 姜明光 Biological liquid compound fertilizer containing monosilicic acid, N, P and K
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CN101698627A (en) * 2009-11-25 2010-04-28 北京理工大学 Multifunctional microbial fertilizer and preparation method thereof
CN102399839A (en) * 2011-05-13 2012-04-04 中国海洋大学生物工程开发有限公司 Production process of bacillus psychrosaccharolyticus extracellular polysaccharide and application thereof
CN102899264A (en) * 2012-06-25 2013-01-30 石河子大学 Microbial fertilizer and its preparation method and use

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CN104262057A (en) * 2014-09-29 2015-01-07 石河子大学 Rhizosphere acid fertilizer and preparation method thereof
CN104817363A (en) * 2014-10-09 2015-08-05 北京绿可欣生物科技有限公司 Humic acid water-soluble fertilizer, and preparation and application methods thereof
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