KR102259326B1 - Formulation including Bacillus mesonae and antioxidant material of plant or plant production promoting method using thereof - Google Patents

Abstract

According to an embodiment of the present invention, there is provided a liquid formulation for enhancing antioxidant substances that can be treated in tomatoes or strawberries including Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP).

Description

Liquid formulation containing Bacillus mesonae strain and method of promoting antioxidant material or production of plants using the same {Formulation including Bacillus mesonae and antioxidant material of plant or plant production promoting method using thereof}

The present invention relates to a liquid formulation containing a Bacillus mesonae strain and a method for increasing the antioxidant material or production of plants using the same, and a liquid formulation containing the Bacillus mesonae strain, and an antioxidant material of a plant using the liquid formulation It relates to a method of enhancing the content and yield of

In order to replace chemical fertilizers and increase the production of crops, research on microbial agents for promoting plant growth has been actively conducted. For example, it is known that microorganisms such as rhizomes symbiotic in the legume family, Pseudomonas genus, Azotobacter genus, Bacillus genus, Azosperylum, etc. help plants grow and increase production.

Currently, both farmers and consumers prefer products that are differentiated from crops such as general vegetables or fruits, and interest in vegetables or fruits containing a large amount of functional substances is increasing. Accordingly, research for enhancing functional substances in crops is also gradually increasing. Therefore, there is a need to develop a microbial agent having a functional ingredient enhancing activity in order to create added value as well as a simple increase in crop production.

1. Korean Patent Publication No. 10-2013-0032100 (2013.04.01)

The technical problem to be solved by the present invention is to provide a liquid formulation of a strain excellent in the effect of enhancing the antioxidant functional components of plants.

In addition, the technical problem to be solved by the present invention is to provide a liquid formulation for enhancing the content of antioxidant functional components of plants using the strain, and a method for enhancing the functionality of plants using the same.

According to an embodiment of the present invention, there is provided a liquid formulation for enhancing antioxidant substances that can be treated in tomatoes or strawberries including Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP).

In one embodiment of the present invention, the tomato may be a jujube cherry tomato or a ripe tomato.

In one embodiment of the present invention, the antioxidant material may be at least one of polyphenol, lycopene, and beta-carotene.

In one embodiment of the present invention, the antioxidant material may be an antioxidant enzyme. The antioxidant enzyme may be at least one of catalase, superoxide dismutase, and glutathione peroxidase.

One embodiment of the present invention provides a liquid formulation for improving production that can be processed on tomatoes, strawberries, and cucumbers, including Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP).

An embodiment of the present invention includes a method for enhancing antioxidant substances of tomatoes or strawberries, wherein the antioxidant substances of tomatoes or strawberries are Bacillus mesonae (Bacillus mesonae) H20-5 strain (Accession No.: KACC 81041BP) containing a liquid formulation The content can be enhanced by preparing and treating the liquid preparation on tomatoes or strawberries. In one embodiment of the present invention, the liquid agent may be irrigated on the plant.

One embodiment of the present invention includes a method of increasing the production of at least one of tomatoes, strawberries, and cucumbers, and the production is Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP) Prepare a liquid containing And it can be enhanced by treating at least one of tomatoes, strawberries, and cucumbers with the liquid agent.

The liquid formulation of Bacillus mesonae of the present invention is remarkably excellent in the effect of increasing the commercial yield of cherry tomatoes, ripe tomatoes, strawberries and cucumber plants. Therefore, by treating the plant with the liquid formulation of Bacillus mesonae of the present invention, it is possible to remarkably improve the value and marketability of the plant.

1 is a phylogenetic analysis diagram comparing the 16s rRNA base sequence of the strain of the present invention with other Bacillus strains.
2 to 4 are graphs comparing by measuring the antioxidant enzyme activity of Bacillus mesonae strain and Bacillus balismotis, Figure 2 is the degree of CAT activation, Figure 3 is the degree of SOD activation, Figure 4 is the degree of GPx activation will show
5 to 7 are graphs comparing the contents of lycopene, beta-carotene, and polyphenols measured when a liquid formulation containing a Bacillus mesonae strain and a Bacillus balismotis strain, respectively, is treated on a tomato.
8 is a graph showing the content of polyphenols in the cherry tomatoes of the control group and the cherry tomatoes treated with a liquid formulation containing the Bacillus mesonae strain.
9 is a graph showing the content of polyphenols in strawberries treated with a liquid formulation containing a Bacillus mesonae strain and strawberries in a control group.
10 is a graph showing the content of lycopene in the cherry tomatoes of the control group and the cherry tomatoes treated with a liquid formulation containing the Bacillus mesonae strain.
11 is a graph showing the content of lycopene in strawberries treated with a liquid formulation containing a Bacillus mesonae strain and a control strawberry.

In the present invention, various modifications may be made and various forms may be applied, and specific embodiments will be illustrated in the drawings and described in detail in the text. However, this is not intended to limit the present invention to a specific form disclosed, it should be understood to include all changes, equivalents, and substitutes included in the spirit and scope of the present invention.

The present invention relates to a liquid preparation containing Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP) and a method for enhancing the antioxidant content and yield of plants using the same.

Bacillus mesonae H20-5 strain according to an embodiment of the present invention is characterized in that it has 16S rRNA of the nucleotide sequence shown in SEQ ID NO: 1.

1 is a phylogenetic analysis diagram comparing the 16s rRNA base sequence of the strain of the present invention with other Bacillus strains.

As used herein, "plant" refers to any living organism belonging to the plant kingdom (i.e., any genus/species within the plant kingdom), such as, but not limited to, trees, herbs, shrubs, grasses, vines, ferns, mosses and green algae. does not For example, plants in which antioxidant substances or yields are enhanced by a composition comprising a Bacillus mesonae strain or a Bacillus mesonae strain according to an embodiment of the present invention may include tomatoes, strawberries, cucumbers, and the like. Here, the tomatoes may include, for example, jujube cherry tomatoes, ripe tomatoes, and the like.

Hereinafter, for convenience of description, the Bacillus mesonae strain or composition comprising the same according to an embodiment of the present invention will be described as an example applied to tomatoes, strawberries, and cucumbers.

In one embodiment of the present invention, the antioxidant substances of plants may include lycopene, polyphenols, beta-carotene, and the like.

Lycopene is a kind of carotenoid pigment and is one of the phytochemicals found in red plants such as tomatoes and strawberries. Lycopene is the most common carotenoid in the human body and one of the most potent carotenoid antioxidants. Lycopene has the effect of discharging free radicals generated in cell metabolism out of the body, and its antioxidant action is twice that of beta-kiritin and 100 times that of vitamin E. It has the strongest antioxidant action among those found in food. In addition, lycopene is known to have a great preventive effect on prostate and breast cancer, which occurs when there is a problem with hormone metabolism.

Polyphenols are one of the representative antioxidants, and they play a role in preventing oxidation and aging of cells or tissues by removing free radicals in the human body. There are thousands of types of polyphenols, and many flavonoids in fruits, isoflavones in soybeans, and catechins in green tea are types of polyphenols. In addition, polyphenols are known to have an effect of preventing inflammation in the body and protecting cells in the body, and most of them are consumed as food.

Beta-carotene is a carotene present in a plant living body, and as a precursor of vitamin A, one beta-carotene molecule is converted into two vitamin A molecules as needed in the living body, thereby helping human intake of nutrients. Beta-carotene is one of the antioxidants that prevents the oxidation or aging of cells in the human body, and it is known that the intake of plants containing beta-carotene significantly reduces the incidence of lung cancer.

The present invention provides a composition for enhancing the functional component of a plant liquid formulation containing the Bacillus mesonae strain. The composition may include the Bacillus mesonae strain itself, its culture medium, formulation, or both as an active ingredient. The functional component may be an antioxidant component such as lycopene, beta-carotene, and polyphenol.

In one embodiment, the plant that can be treated with the composition for enhancing the antioxidant functional component of the plant may be a tomato (jujube cherry tomatoes, ripe tomatoes, etc.), strawberries, cucumbers, etc., but is not limited thereto. In one embodiment, the composition for enhancing the antioxidant functional component of the plant may be treated on tomatoes or strawberries, and the content of antioxidants such as lycopene, polyphenols, and beta-carotene in tomatoes or strawberries may be remarkably enhanced. However, the plant to which the Bacillus mesonae strain or composition comprising the same according to an embodiment of the present invention is applied may be variously changed in addition to tomatoes, strawberries, and cucumbers. However, it will be understood that there is a difference in the degree of enhancement of antioxidant substances and yields that can be obtained from a Bacillus mesonae strain or a composition comprising the same depending on the species.

In an embodiment, the liquid formulation or composition for promoting plant growth according to the present invention may further include any one or more selected from the group consisting of a plant growth regulator, a plant growth stimulator, and a plant nutrient.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1. Method for preparing a liquid formulation of Bacillus mesonae strain

After culturing the strain of the present invention in tryptic soy agar at 28° C. for 24 hours, a single colony was cultured in 100 ml tryptic soy broth at 28° C., 150 rpm for 48 hours with shaking, and the concentration of the culture medium compared to the control was OD ₆₀₀ = It was adjusted to 0.2 and used as a liquid formulation (TP-H20-5).

Example 2. Increase in antioxidant enzyme activity in tomato plants by treatment with liquid formulations containing Bacillus mesonae strains

This example is to confirm whether the Bacillus mesonae strain activates the antioxidant enzyme of tomato, and whether the strain of Bacillus genus has the same effect. When plants receive various stresses while growing, they are accompanied by oxidative stress caused by active oxygen. At this time, antioxidant enzymes are involved in the reaction mechanism to reduce active oxygen.

The strains tested in this example were Bacillus mesonae H20-5 and Bacillus vallismortis BS07M (Bacillus vallismortis).

Bacillus ballismotis is known as a strain that promotes plant growth, and this content is disclosed in Korean Patent Publication No. 10-2013-0032100 (2013.04.01). In this experiment, an experiment was performed using a strain corresponding to the Bacillus ballismotis (name of deposit institution: Korea Research Institute of Bioscience and Biotechnology; accession number: KCTC11991BP; accession date: 20110728) known in the above patent document. The liquid formulation containing the strain of Bacillus balismotis was prepared in substantially the same manner as the liquid formulation containing the strain of Bacillus mesonae.

In this experiment, grown adult tomatoes were treated with a liquid formulation of Bacillus balismotis strain BS07M, and a liquid formulation of Bacillus mesonae H20-5 strain.

At the time of strain treatment, tomatoes were drenched using liquid preparations of Bacillus mesonae strain and Bacillus vallismottis strain. After the tomato seedlings were planted in the soil for plant cultivation, 1 liter of liquid formulation per 200 pyeong was diluted 500 times and treated with drip irrigation twice at intervals of 2 weeks.

The control group is a conventional cultivation case treated with water without Bacillus mesonae strain and Bacillus ballismotis strain, and the remaining conditions are experimental results of tomatoes maintained the same as BS07M and H20-5.

In this experimental example, in order to measure the enzyme activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx), plant tissue (1 g) from tomato It was sampled and used after quenching in liquid nitrogen. For the CAT activity assay, after grinding the sampled plants in 5 ml of a solution of 1 mM EDTA in cold 50 mM potassium phosphate buffer (pH = 7.0), 4 ^o C for 15 minutes at 12000 rpm centrifuged.

CAT enzyme activity was measured with a catalase assay kit (item No. 707002, Cayman Chemical Company, USA) using the supernatant after centrifugation.

To measure the activity of SOD, the sampled plant tissue was ground in 5 ml of a cold solution of 20 mM HEPES buffer (pH = 7.2), 1 mM EGTA, 210 mM mannitol, and 70 mM sucrose mixed at 2000 rpm at ^{4 ° C.} centrifuged for min. The SOD enzyme activity was measured with a superoxide dismutase assay kit (item No. 706002, Cayman Chemical Company, USA) using the supernatant after centrifugation. GPx activity was measured with a glutathione peroxidate assay kit (item No. 703102, Cayman Chemical Company, USA). This experiment was repeated 5 times each over 2 times.

Table 1 is a comparison by measuring the antioxidant enzyme activity of Bacillus mesonae strains and Bacillus balismotis. 2 to 4 are graphs comparing by measuring the antioxidant enzyme activity of Bacillus mesonae strain and Bacillus balismotis, Figure 2 is the degree of CAT activation, Figure 3 is the degree of SOD activation, Figure 4 is the degree of GPx activation will show 1 to 3 , an asterisk indicated on the bar graph indicates a significant difference (P<0.05), and a portion indicated with a bar indicates the standard error.

In Table 1, BS07M is an experimental result of a tomato treated with a Bacillus ballismotis strain liquid formulation, H20-5 is an experimental result of a tomato treated with a Bacillus mesonae strain, and the control group is a Bacillus mesonae strain and Bacillus It is a conventional cultivation case treated with water without a ballismotis strain, and the remaining conditions are the experimental results of tomatoes maintained the same as BS07M and H20-5.

Treatment CAT activity
(nmol/min/mg protein) SOD activity
(U/mg protein) GPx activity
(nmol/min/mg protein) Control 4.39 ± 0.74a 0.83 ± 0.02 15.48 ± 1.16 BS07M 4.61 ± 1.50 0.92 ± 0.03 15.56 ± 2.18 TP-H20-5 6.15 ± 0.68* 1.16 ± 0.11* 19.73 ± 3.31*

Referring to Table 1, it was confirmed that the activity of SOD, CAT, GPx was remarkably increased in the tomato treated with the liquid formulation containing the Bacillus mesonae strain as a result of the experiment. Through this, the liquid formulation containing the Bacillus mesonae strain is expected to directly and/or indirectly control the content of active oxygen in the body by increasing the activity of antioxidant enzymes when treated with tomatoes. In contrast, in the case of tomatoes treated with a liquid formulation containing a Bacillus balismotis strain, although it was observed that the activity of SOD, CAT, and GPx increased even in trace amounts, the difference was not significant compared to the control group. Through this, it can be confirmed that even the strains of the genus Basalus show different degrees of antioxidant activity.

Example 3. Effect of increasing the content of tomato lycopene, beta-carotene, and polyphenols by treatment with Bacillus mesonae strain

This example is to confirm whether the content of antioxidants lycopene, beta-carotene, and polyphenols in tomatoes increases when the liquid formulation of the Bacillus mesonae strain is treated on the tomato.

The strains tested in this example were Bacillus mesonae H20-5 and Bacillus vallismortis BS07M (Bacillus vallismortis) the same as in Example 2. The liquid formulation containing the strain of Bacillus balismotis was prepared in substantially the same manner as the liquid formulation containing the strain of Bacillus mesonae.

This experiment was performed by the greenhouse test method, which is the same as the farmhouse test. In this experiment, grown adult tomatoes were treated with Bacillus balismotis strain BS07M liquid formulation, and Bacillus mesonae strain.

At the time of strain treatment, tomatoes were drenched using liquid preparations of Bacillus mesonae strain and Bacillus balismotis strain. After the tomato seedlings were planted in the soil for plant cultivation, 1 liter of liquid formulation per 200 pyeong was diluted 500 times and treated with drip irrigation twice at intervals of 2 weeks.

To evaluate the lycopene content, 0.2 g of the freeze-dried sample was extracted with 10 ml of acetone-hexane (4:6) solution, and the extracted sample was centrifuged at 3000 rpm for 5 minutes at 4° C. to collect the supernatant. The collected supernatant was quantified by the Nagata and Yamashita (1992) method by measuring absorbance at 663 nm, 645 nm, 505 nm, and 453 nm using a spectrophotometer.

After freeze-drying the fruit, beta-carotene was extracted with a 4:6 mixture of acetone-hexane (acetone-hexan; 4:6), ^{centrifuged at 4 o} C at 3000 rpm for 5 minutes, and absorbance 663 nm, 645 nm, 505 nm , measured at 453 nm and evaluated as β-carotene (mg/100g dry weight) = 0.216xA663-1.22xA645-0.304xA505+0.452xA453.

In order to evaluate the polyphenol content, extraction was performed by extracting 0.5 g of a freeze-dried sample with 20 ml of 50% methanol and stirring for 1 hour with 20 ml of 70% acetone according to the method of Vasco et al. (2008). A second extraction was performed. . After centrifuging the extract at 4000 rpm for 15 minutes, 50 ml of distilled water was added in a volumetric flask to prepare an extract to be used for analysis. 0.5 ml of the extract was mixed with 0.5 ml of Folin-Cioaltew reagent, stirred for 3 minutes, and 10 ml of sodium carbonate solution (pure 75 g/L) was added. The final volume was adjusted to 25 ml with distilled water and reacted at room temperature for 1 hour. Thereafter, the absorbance was measured at a wavelength of 750 nm using a spectrophotometer. As a standard curve, gallic acid was used.

Tables 2 to 4 compare the contents of lycopene, beta-carotene, and polyphenols by measuring the contents of lycopene, beta-carotene, and polyphenols when a liquid formulation containing a Bacillus mesonae strain and a Bacillus balismotis strain, respectively, is treated on a tomato. 5 to 7 are graphs showing Tables 2 to 4, respectively. 5 to 7 , an asterisk indicated on the bar graph indicates a significant difference (P<0.05), and a portion indicated with a bar indicates the standard error.

In Tables 2 to 4, BS07M is the experimental result of the tomato treated with the Bacillus balismotis strain liquid formulation, H20-5 is the experimental result of the tomato treated with the Bacillus mesonae strain, and the control group is Bacillus mesona. As a conventional cultivation case treated with water without strain and Bacillus ballismotis strain, the remaining conditions are the experimental results of tomatoes maintained the same as BS07M and H20-5.

Referring to Tables 2 to 4, and FIGS. 5 to 7, as a result of the experiment, in the case of tomatoes treated with a liquid formulation containing a Bacillus mesonae strain, lycopene, beta-carotene, and polyphenols all significantly increased in content Confirmed. Through this, it can be confirmed that the content of antioxidants, in particular, lycopene, beta-carotene, and polyphenols, is remarkably increased when the liquid formulation containing the Bacillus mesonae strain is treated on tomatoes.

In contrast, in the tomato treated with the liquid formulation containing the Bacillus balismotis strain, in the case of lycopene, although there is a significant increase in the content compared to the control, compared to the tomato treated with the liquid formulation containing the Bacillus mesonae strain The increase was not large. In addition, in the tomato treated with the liquid formulation containing the Bacillus balismotis strain, in the case of beta-carotene and polyphenol, a significant increase in content compared to the control was not observed. Through this, it was confirmed that even the strains of the genus Basalus had different antioxidant-increasing effects.

treatment Mean±SE LSD Control 145.38±3.78 C BS07M 168.09±3.62 B TP-H20-5 185.13±1.91 A

treatment Mean±SE LSD Control 36.51±0.73 B BS07M 38.43±1.47 B TP-H20-5 41.35±0.70 A

treatment Mean±SE LSD Control 22.1±0.15 B BS07M 22.9±0.34 B TP-H20-5 23.2±0.3 A

Example 4: Effect of increasing polyphenol content of cherry tomatoes and strawberries by liquid formulation containing Bacillus mesonae strain

This example was carried out to confirm whether the antioxidant content in each cherry tomato and strawberry was enhanced when the liquid formulation containing the Bacillus mesonae strain was treated on the cherry tomatoes and strawberries.

Using a liquid formulation of the Bacillus mesonae strain, each of the cherry tomatoes and strawberries were drenched. After planting the jujube cherry tomato seedlings in the soil cultivated in facilities, 1 liter of liquid formulation per 200 pyeong was diluted 500 times and treated with drip irrigation twice at intervals of 2 weeks.

At the same time, the same amount of water was treated in conventional (control) cultivation.

Jujube cherry tomatoes and strawberries were harvested from December 23, 2018 to March 30, 2019, and from December 20, 2018 to February 28, 2019, from 2 months after the liquid formulation was processed, respectively. Cherry tomato fruit was randomly sampled in 4 replicates and strawberries in 5 replicates.

The sampled fruits were freeze-dried under reduced pressure (0.100mbar) at -40°C for 7 days, and the freeze-dried fruits were pulverized with a mortar and filtered through 250μm (opening micrometer, tyler equivalent 60 mesh) and the pulverized particles were the same size. was prepared and used for analysis.

For polyphenol extraction, 0.5 g of a freeze-dried sample was extracted with 20 ml of 50% methanol according to the method of Vasco et al. (2008), followed by a second extraction by stirring with 20 ml of 70% acetone for 1 hour. After centrifuging the extract at 4000 rpm for 15 minutes, 50 ml of distilled water was added in a volumetric flask to prepare an extract to be used for analysis. 0.5 ml of the extract was mixed with 0.5 ml of Folin-Cioaltew reagent, stirred for 3 minutes, and 10 ml of sodium carbonate solution (pure 75 g/L) was added. The final volume was adjusted to 25 ml with distilled water and reacted at room temperature for 1 hour. Thereafter, the absorbance was measured at a wavelength of 750 nm using a spectrophotometer. As a standard curve, gallic acid was used.

8 is a graph showing the content of polyphenols in cherry tomatoes treated with a liquid formulation (represented as TP-H20-5) containing Bacillus mesonae strains and the cherry tomatoes of the control, FIG. It is a graph showing the content of polyphenols in strawberries treated with a liquid formulation (represented by TP-H20-5) containing Bacillus mesonae strains and strawberries in the control group.

Referring to FIGS. 8 and 9 , it can be confirmed that cherry tomatoes and strawberries treated with a liquid formulation (represented by TP-H20-5) containing the Bacillus mesonae strain exhibit a significantly higher polyphenol content compared to the control group. have.

In particular, referring to FIG. 8, as a result of measuring the polyphenol content in the date cherry tomatoes, the polyphenol content of the date cherry tomatoes treated with the liquid formulation containing the Bacillus mesonae strain was 1.43±0.06 mM/g (dry weight). ), on the other hand, the polyphenol content of Jujube cherry tomatoes during conventional cultivation (control) is 1.20±0.02mM/g (dry weight), and when a liquid formulation containing Bacillus mesonae strain is treated, the polyphenol content is higher than that of conventional culture. It increased by 19.17%.

Referring to FIG. 9 , the polyphenol content of the strawberry treated with the liquid formulation containing the Bacillus mesonae strain is 2.14±0.08mM/ g (dry weight), whereas the polyphenol content of conventionally grown (control) strawberries was 1.88±0.03mM/g (dry weight), and 13.83% polyphenol compared to conventional when treated with a liquid formulation containing Bacillus mesonae strain The phenol content increased.

Example 5: Effect of increasing lycopene content of cherry tomatoes and strawberries by liquid formulation containing Bacillus mesonae strain

It was sampled and freeze-dried in the same manner as described in Example 4, and used to measure the lycopene content. 0.2 g of the freeze-dried sample was extracted with 10 ml of acetone-hexane (4:6) solution, and the extracted sample was centrifuged at 3000 rpm for 5 minutes at 4° C. to collect the supernatant. The collected supernatant was quantified by the Nagata and Yamashita (1992) method by measuring absorbance at 663 nm, 645 nm, 505 nm, and 453 nm using a spectrophotometer.

Figure 10 is a graph showing the content of lycopene in the date cherry tomatoes treated with a liquid formulation (represented by TP-H20-5) containing the Bacillus mesonae strain and the control date cherry tomatoes, Figure 11 is Bacillus It is a graph showing the content of lycopene in strawberries treated with a liquid formulation (represented by TP-H20-5) containing the Mesonae strain and strawberries in the control group.

10 and 11 , it can be seen that cherry tomatoes and strawberries treated with a liquid formulation (represented by TP-H20-5) containing the Bacillus mesonae strain exhibit a significantly higher lycopene content compared to the control group. .

Referring to FIG. 10, as a result of measuring the lycopene content in the cherry tomatoes, the lycopene content of the cherry tomatoes treated with the liquid formulation containing the Bacillus mesonae strain was 3.13±0.03 μg/g (dry weight), whereas The lycopene content in conventional cultivation (control) was 2.69±0.02 μg/g (dry weight), and when the liquid formulation containing the Bacillus mesonae strain was treated, the lycopene content increased by 16.36% compared to the conventional one.

Referring to FIG. 11 , the lycopene content in strawberries was 17.74±0.001 ng/g (dry weight) when the liquid formulation containing the Bacillus mesonae strain was treated, whereas the lycopene content in conventional cultivation (control) was 8.97±0.001 When the liquid formulation containing the Bacillus mesonae strain as ng/g (dry weight) was treated, the lycopene content was increased by 97.77% compared to conventional practice.

Example 6: Effect of increasing yield of facility-cultivated crops (jujube cherry tomatoes, ripe tomatoes, strawberries, cucumbers) by a liquid formulation containing a Bacillus mesonae strain

The liquid formulation containing the Bacillus mesonae strain was irrigated with those described in Example 4, respectively, at 2 week intervals after the jujube cherry tomatoes, ripe tomatoes, strawberries, and cucumbers were planted.

Table 5 shows the results of comparison with the control group (conventional cultivation, control) by measuring the yields of jujube cherry tomatoes, strawberries, ripe tomatoes, and cucumbers in facility cultivation fields.

process Yield (kg), (increase %) Jujube Cherry Tomato ripe tomatoes Strawberry cucumber Control 1695 6050 114.3 1522 TP-H20-5 2058.5 (21.4%) 6610 (9.3%) 242.2 (120.6%) 1742 (14.5%)

Referring to Table 5, as a result of evaluating the total yield of Jujube cherry tomatoes during the harvest season (December 23 to March 30, 2018), the total yield per 200 pyeong in conventional cultivation was 1695 kg, whereas Bacillus mesonae strain In the case of treatment with liquid formulations containing , the total yield per 200 pyeong was 2058.5 kg, which increased by 21.4% compared to the conventional one. As a result of evaluating the total yield of ripe tomatoes during the harvest period (February 15-April 29, 2019), the total yield per 200 pyeong was 6050 kg in conventional cultivation, whereas the total yield per 200 pyeong was 6610 kg when treated. It increased by 9.3% compared to the conventional one.

As a result of evaluating the total harvest of strawberries during the harvest period (December 20, 2018 - February 28, 2019), the total yield per 150 pyeong was 114.3 kg in conventional cultivation, whereas the total yield per 150 pyeong was 252.2 kg As a result, it increased by 120.6% compared to conventional practice.

As a result of evaluating the total yield of cucumbers during the harvesting period (October 21, 2018 - November 28, 2018), the total yield per 200 pyeong was 1522 kg in conventional cultivation, whereas the total yield per 200 pyeong was 1742 kg in the case of treatment. As a result, it increased by 14.5% compared to conventional practice.

In conclusion, it was confirmed that the microbial liquid formulation containing the Bacillus mesonae strain of the present invention significantly enhances the antioxidant functional ingredients-lycopene and polyphenol content of crops such as cherry tomatoes and strawberries, and also It was confirmed that harvest yields were significantly increased compared to conventional crops, such as jujube cherry tomatoes, ripe tomatoes, strawberries and cucumbers, which are planted crops.

Results data of all the above experiments were analyzed for variation using SAS software (SAS Institute, Cary, NC, USA). Significant differences according to the treatment means of each sample were determined using the Least Significance Difference (LSD) Test at P < 0.05.

Although the above has been described with reference to the preferred embodiment of the present invention, those skilled in the art or those having ordinary knowledge in the technical field will not depart from the spirit and technical scope of the present invention described in the claims to be described later. It will be understood that various modifications and variations of the present invention can be made without departing from the scope thereof.

Accordingly, the technical scope of the present invention should not be limited to the contents described in the detailed description of the specification, but should be defined by the claims.

Name of deposit institution: Agricultural Biotechnology Research Institute

Accession number: KACC81041

Deposit date: 20170109

<110> REPUBLIC OF KOREA(MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Bacillus mesonae strain promoting tolerance of plants and use thereof <130> P18-104 <150> KR 10-2017-0046958 <151> 2017-04-11 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1519 <212> DNA <213> Unknown <220> <223> 16S rRNA of Bacillus mesonae H20-5 <400> 1 ttttgagttt tagaatctgg tcaggacgaa cgctggcggc gtgcctaata catgcaagtc 60 gagcgaactt gcgggagctt gctcccaaaa gttagcggcg gacgggtgag taacacgtgg 120 gcaacctgcc tgtaagactg ggataacttc gggaaaccgg agctaatacc ggataattct 180 tatcaacaca tgttggtaag ctgaaagtcg gtttcggctg acacttacag atgggcccgc 240 ggcgcattag ctagttggtg aggtaacggc tcaccaaggc aacgatgcgt agccgacctg 300 agagggtgat cggccacact gggactgaga cacggcccag actcctacgg gaggcagcag 360 tagggaatct tccacaatgg acgaaagtct gatggagcaa cgccgcgtga gcgatgaagg 420 ccttcgggtc gtaaagctct gttgttaggg aagaacaagt atcggagtaa ctgccggtac 480 cttgacggta cctaaccaga aagccacggc taactacgtg ccagcagccg cggtaatacg 540 taggtggcaa gcgttgtccg gaattattgg gcgtaaagcg cgcgcaggcg gtcctttaag 600 tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt ggaaactggg ggacttgagt 660 gcagaagagg aaagcggaat tccacgtgta gcggtgaaat gcgtagagat gtggaggaac 720 accagtggcg aaggcggctt tctggtctgt aactgacgct gaggcgcgaa agcgtgggga 780 gcaaacagga tagataccc tggtagtcca cgccgtaaac gatgagtgct aagtgttaga 840 gggtttccgc cctttagtgc tgcagctaac gcattaagca ctccgcctgg ggagtacggc 900 cgcaaggctg aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt 960 taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctctgaca ctcctagaga 1020 taggactttc cccttcgggg gacagagtga caggtggtgc atggttgtcg tcagctcgtg 1080 tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttgatcttag ttgccagcat 1140 tcagttgggc actctaaggt gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc 1200 aaatcatcat gccccttatg acctgggcta cacacgtgct acaatggatg gtacaaaggg 1260 ctgcaaaacc gcaaggtcta gccaatccca taaaaccatt ctcagttcgg attgcaggct 1320 gcaactcgcc tgcatgaagc cggaatcgct agtaatcgcg gatcagcatg ccgcggtgaa 1380 tacgttcccg ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag 1440 tcggtggggt aaccgtaagg agccagccgc ctaaggtggg acagatgatt ggggtgaagt 1500 cgtaacaggg tagccgtaa 1519

Claims (11)

A liquid formulation for enhancing antioxidant substances that can be treated in strawberries containing Bacillus mesonae H20-5 strain (Accession No.: KACC 81041BP). delete According to claim 1, wherein the antioxidant is at least one of polyphenol, lycopene, beta-carotene liquid formulation for enhancing antioxidant substances. The liquid formulation for enhancing an antioxidant according to claim 1, wherein the antioxidant is an antioxidant enzyme. The method of claim 4,
The antioxidant enzyme is at least one of catalase, superoxide dismutase, and glutathione peroxidase. Bacillus mesonae (Bacillus mesonae) containing H20-5 strain (Accession No.: KACC 81041BP), a liquid formulation for improving production that can be processed on strawberries and cucumbers. Preparing a solution containing Bacillus mesonae (Bacillus mesonae) H20-5 strain (Accession No.: KACC 81041BP); and
A method for enhancing antioxidant substances in strawberries, comprising the step of treating the liquid preparation on strawberries. The method of claim 7,
The liquid agent is an antioxidant enhancement method that is treated with irrigation. The method of claim 7,
The antioxidant is at least one of polyphenol, lycopene, and beta-carotene. Preparing a solution containing Bacillus mesonae (Bacillus mesonae) H20-5 strain (Accession No.: KACC 81041BP); and
A method for increasing production of at least one of strawberries and cucumbers, comprising the step of treating the liquid agent on at least one of strawberries and cucumbers. delete

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