CN102432399A - Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof - Google Patents
Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof Download PDFInfo
- Publication number
- CN102432399A CN102432399A CN2011102749410A CN201110274941A CN102432399A CN 102432399 A CN102432399 A CN 102432399A CN 2011102749410 A CN2011102749410 A CN 2011102749410A CN 201110274941 A CN201110274941 A CN 201110274941A CN 102432399 A CN102432399 A CN 102432399A
- Authority
- CN
- China
- Prior art keywords
- microbial inoculum
- biological organic
- organic fertilizer
- liquid preparation
- complex microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 88
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 61
- 240000007594 Oryza sativa Species 0.000 title claims abstract description 45
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 45
- 235000009566 rice Nutrition 0.000 title claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 239000000203 mixture Substances 0.000 claims abstract description 36
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 31
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 28
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 27
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 24
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 24
- 239000000126 substance Substances 0.000 claims abstract description 12
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 10
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 10
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 10
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 9
- 239000002068 microbial inoculum Substances 0.000 claims description 81
- 244000005700 microbiome Species 0.000 claims description 46
- 239000003795 chemical substances by application Substances 0.000 claims description 38
- 241000196324 Embryophyta Species 0.000 claims description 24
- 241000186660 Lactobacillus Species 0.000 claims description 20
- 229940039696 lactobacillus Drugs 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 19
- 230000004151 fermentation Effects 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 15
- 210000003608 fece Anatomy 0.000 claims description 15
- 230000000813 microbial effect Effects 0.000 claims description 15
- 239000010871 livestock manure Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 235000012054 meals Nutrition 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 241000195940 Bryophyta Species 0.000 claims description 10
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 10
- 235000012343 cottonseed oil Nutrition 0.000 claims description 9
- 239000003415 peat Substances 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 235000019764 Soybean Meal Nutrition 0.000 claims description 4
- 239000004455 soybean meal Substances 0.000 claims description 4
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 3
- 244000105624 Arachis hypogaea Species 0.000 claims description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 3
- 235000018262 Arachis monticola Nutrition 0.000 claims description 3
- 235000019779 Rapeseed Meal Nutrition 0.000 claims description 3
- 235000020232 peanut Nutrition 0.000 claims description 3
- 239000004456 rapeseed meal Substances 0.000 claims description 3
- 241000272525 Anas platyrhynchos Species 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 230000008635 plant growth Effects 0.000 claims 1
- 239000006041 probiotic Substances 0.000 claims 1
- 230000000529 probiotic effect Effects 0.000 claims 1
- 235000018291 probiotics Nutrition 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 50
- 239000002689 soil Substances 0.000 abstract description 24
- 239000002054 inoculum Substances 0.000 abstract description 13
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 230000035558 fertility Effects 0.000 abstract description 5
- 229910052700 potassium Inorganic materials 0.000 abstract description 5
- 235000013619 trace mineral Nutrition 0.000 abstract description 4
- 239000011573 trace mineral Substances 0.000 abstract description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 239000011591 potassium Substances 0.000 abstract description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000002969 morbid Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 33
- 230000001580 bacterial effect Effects 0.000 description 16
- 239000000843 powder Substances 0.000 description 11
- 230000004913 activation Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 238000010899 nucleation Methods 0.000 description 10
- 239000001888 Peptone Substances 0.000 description 9
- 108010080698 Peptones Proteins 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 9
- 238000012856 packing Methods 0.000 description 9
- 235000019319 peptone Nutrition 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 239000007787 solid Substances 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241001052560 Thallis Species 0.000 description 4
- 244000269722 Thea sinensis Species 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 4
- 239000006071 cream Substances 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000002594 sorbent Substances 0.000 description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical class [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 244000061458 Solanum melongena Species 0.000 description 3
- 235000002597 Solanum melongena Nutrition 0.000 description 3
- 229910021536 Zeolite Inorganic materials 0.000 description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000010457 zeolite Substances 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 235000019483 Peanut oil Nutrition 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 239000000312 peanut oil Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 229960004249 sodium acetate Drugs 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- JJEJDZONIFQNHG-UHFFFAOYSA-N [C+4].N Chemical compound [C+4].N JJEJDZONIFQNHG-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000012773 agricultural material Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000011091 sodium acetates Nutrition 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- PODWXQQNRWNDGD-UHFFFAOYSA-L sodium thiosulfate pentahydrate Chemical compound O.O.O.O.O.[Na+].[Na+].[O-]S([S-])(=O)=O PODWXQQNRWNDGD-UHFFFAOYSA-L 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- -1 subtilis Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Abstract
The invention discloses a biological organic fertilizer for improving the stress resistance and the output of paddy rice and a preparation method thereof. The biological organic fertilizer comprises the following components: an organic mixture adsorbed with a composite microbe liquid preparation, an organic substance, a Bacillus subtilis inoculum, a Bacillus licheniformis inoculum, a Saccharomyces cerevisiae inoculum, a Rhodopseudomonas palustris inoculum, Lactobacillus plantarum and Lactobacillus acidophilus. The biological organic fertilizer of the invention is rich in beneficial microbe florae and major and trace elements needed by the growth of various crops, so the ecological florae and the trace elements of soil can be supplemented and adjusted, phosphor dissolving and potassium releasing capabilities of soil are enhanced, effective nutrients can be strongly decomposed, converted and synthesized, the fertilizer utilization rate is improved, physical and chemical properties of soil are improved, the granular structure formation is promoted, hardened and morbid soil is restored, the soil fertility is increased, various soil-borne diseases are inhibited, the growth and the fructification of the crops are promoted, and the stress resistance and the output of the crops are substantially improved.
Description
Technical field
The present invention relates to a kind of biological organic fertilizer and preparation method thereof, relate in particular to a kind of biological organic fertilizer that improves paddy rice anti contravariance property and output, the invention still further relates to the preparation method of this biological organic fertilizer, belong to biological organic fertilizer and preparation field thereof.
Background technology
Paddy rice accounts for 40% of total output of grain as the first food crop of China.Rice Production is not only being undertaken the important task of guaranteeing Chinese grain security, also bears and realizes that kind of a grain synergy, rice farming increase income and push forward comprehensively the magnificent mission of new countryside construction.Though China also faces new challenges having obtained remarkable achievement aspect Rice Production and the scientific research at present.At first be that the population growth is not eased with the contradiction that reduces of ploughing.It is predicted that to the year two thousand thirty, Chinese population will reach 1,600,000,000, will further increase the demand of grain.
Because the various places Economic development needs, a large amount of farmlands are taken by all kinds of development areas, cause cultivated area constantly to reduce in recent years.2003, the rice in China cultivated area once slided into 3.9 hundred million mu down, and was more young more than 100,000,000 mu than 1976.Secondly, the depth development of paddy rice is seriously checked in environmental change.In recent years, because climatic anomaly, some geographic paddy rice setting percentages constantly descended, the poorest only less than 10%, had had a strong impact on the enthusiasm that the peasant plants grain.Be that early warning system is unsound at last, disease and pest prevention and control system disappearance.Add irrational use agricultural chemicals, rice pest also is the trend that lays particular stress on generation.
Biological organic fertilizer is meant microorganisms with specific functions and serve as one type of fertilizer that has microbial fertilizer and fertilizer effect concurrently of originating and being composited through harmless treatment, the organic materials that becomes thoroughly decomposed with plant and animal residues (like feces of livestock and poultry, agricultural crop straw etc.) mainly.
Biological organic fertilizer can effectively nurse one's health soil, activate that mikrobe enlivens rate in the soil, overcome soil compaction, increase the soil air permeability.Biological organic fertilizer is increase soil fertility reducing fertilizer amount or progressively substituting under the situation of chemical fertilizer, makes food crop, cash crop, greengrocery, melon and fruit class increase production and improve quality of agricultural product significantly.Biological organic fertilizer can also enhancing crop disease resistance and resistance, alleviate disease and soil-borne disease that crop is caused because of continuous cropping, reduces sickness rate; Control to mosaic disease, balck shank, anthrax etc. all has effect preferably, and enhancing crop is to the integrated campaign ability of poor environment simultaneously.
The prior biological organic fertilizer especially is still waiting raising in the aspects such as output and resistance of paddy rice improving crop, and a kind of biological organic fertilizer that can more effectively improve paddy rice anti contravariance property and output need be provided.
Summary of the invention
One of the object of the invention provides a kind of biological organic fertilizer that can effectively improve paddy rice anti contravariance property and output;
Two of the object of the invention provides a kind of method for preparing biological organic fertilizer;
Three of the object of the invention is that above-mentioned biological organic fertilizer is applied to the paddy rice plantation;
Above-mentioned purpose of the present invention realizes through following technical scheme:
A kind of biological organic fertilizer that improves paddy rice anti contravariance property and output comprises following component:
Be adsorbed with the organic mixture of complex microorganism liquid preparation; Organic substance; Bacillus subtilis (Bacillus subtilis) microbial inoculum, Bacillus licheniformis (Bacillus licheniformis) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum; Rhodopseudomonas palustris (Rhodopseudomonas palustris) microbial inoculum, plant lactobacillus (Lactobacillus plantarum) microbial inoculum and Lactobacterium acidophilum microbial inoculum (Lactobacillus acidophilus).
In order to reach better technique effect; Preferably, the weight part of each component is: be adsorbed with organic mixture 8-10 part of complex microorganism liquid preparation, organic substance 30-50 part; Subtilis (Bacillus subtilis) microbial inoculum 10-20 part; Bacillus licheniformis (Bacillus licheniformis) microbial inoculum 7-10 part, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum 7-12 part, Rhodopseudomonas palustris (Rhodopseudomona spalustris) microbial inoculum 4-9; Part, plant lactobacillus microbial inoculum (Lactobacillus plantarum) 4-9 and Lactobacterium acidophilum microbial inoculum (Lactobacillus acidophilus) 4-9 part.
Preferred; The weight part of each component is: be adsorbed with 10 parts of the organic mixtures of complex microorganism liquid preparation, and organic 40 parts, 15 parts of bacillus subtilis microbial agents; 8 parts of bacillus licheniformis agents; 8 parts of yeast saccharomyces cerevisiae bacteria agents, 5 parts of Rhodopseudomonas palustris microbial inoculums, plant lactobacillus microbial inoculum 5 and 4 parts of Lactobacterium acidophilum microbial inoculums (Lactobacillus acidophilus).
Wherein, the described organic mixture that is adsorbed with the complex microorganism liquid preparation prepares according to following method:
(1) preparation complex microorganism liquid preparation: plant lactobacillus (Lactobacillus plantarum) microbial inoculum, Lactobacterium acidophilum (Lactobacillus acidophilus) microbial inoculum and the water part by weight according to 30-50: 30-50: 10-30 is mixed, promptly get; Preferably, plant lactobacillus microbial inoculum, Lactobacterium acidophilum microbial inoculum and the water part by weight according to 40: 40: 20 is mixed;
(2) the complex microorganism liquid preparation is adsorbed in the organic mixture, promptly gets the organic mixture that is adsorbed with the complex microorganism liquid preparation; Preferably, described organic mixture preferably is made up of dregs of beans, pomace, the peat composed of rotten mosses;
In the described organic mixture that is adsorbed with the complex microorganism liquid preparation, the weight part of each component preferably: complex microorganism liquid preparation 2-4 part, dregs of beans 40-60 part, pomace 25-35 part, peat composed of rotten mosses 30-45 part.
Effective bacterium number of the above-mentioned prepared organic mixture that is adsorbed with the complex microorganism liquid preparation that obtains is more than or equal to 3.0 * 10
9The cfu/ gram.
Described organic substance can be various crop seed grouts (pressing the resulting by products in oil back), for example, can be in cottonseed meal, soybean meal, peanut meal or the rapeseed meal any one or multiple, be preferably cottonseed meal or soybean meal; Described organic substance also can be the animal excrement of various fermentation maturities, for example, can be in the animal excrement such as pig manure, chicken manure, duck excrement or sheep excrement of fermentation maturity any one or multiple.
Plant lactobacillus microbial inoculum described in the present invention, Lactobacterium acidophilum microbial inoculum, subtilis, microbial inoculum; Bacillus licheniformis, microbial inoculum, yeast saccharomyces cerevisiae bacteria agent or Rhodopseudomonas palustris microbial inoculum all can prepare according to the conventional bacterial preparation process in this area;
The used microbial strains of the present invention (can available from China Committee for Culture Collection of Microorganisms common micro-organisms center) and other raw material all can be bought from market and obtain, and its specification meets national sector standard.
Another object of the present invention provides a kind of method for preparing above-mentioned biological organic fertilizer.
Preferably, a kind of method for preparing biological organic fertilizer of the present invention may further comprise the steps:
1) preparation subtilis (Bacillus subtilis) microbial inoculum; Bacillus licheniformis (Bacillus Licheniformis Capsules) microbial inoculum; S. cervisiae (Saccharomyces cerevisiae Hansen) microbial inoculum and Rhodopseudomonas palustris (Rhodopseudomonas palustris) microbial inoculum, plant lactobacillus microbial inoculum (Lactobacillus plantarum) and Lactobacterium acidophilum microbial inoculum (Lactobacillus acidophilus).
2) organic substance is pulverized back and the organic mixture that is adsorbed with the complex microorganism liquid preparation; Bacillus subtilis (Bacillus subtilis) microbial inoculum; Bacillus licheniformis (Bacillus licheniformis) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum and Rhodopseudomonas palustris (Rhodopseudomonas palustris) microbial inoculum mix, dry, packing promptly gets.
Biological organic fertilizer method of use of the present invention:, spread fertilizer over the fields best results can be used for base manure aspect the paddy rice plantation and topdressing two kinds of methods.Bio-organic fertilizer of the present invention can be turned over after other fertilizer evenly spreads fertilizer over the fields the surface, soil and make base manure, also can do the base manure ditch spread at seeding time, every mu of ground consumption 4-8 kilogram.
Biological organic fertilizer of the present invention is rich in beneficial microbe colony, and the environmental compatibility of this flora is strong, is prone to give play to the population advantage, and trophic function is strong, and rhizosphere is short living effective, and fertilizer efficiency is high; Be rich in organic and inorganic nutrient, volume is little, be convenient to use, and can satisfy large-scale production and request for utilization; Biological organic fertilizer of the present invention is rich in physiologically active substance; Safe and harmless.
Biological organic fertilizer of the present invention is rich in various crop and grows necessary in a large number and micro-, can replenish and adjust soil ecology flora and trace element, strengthen to dissolve the phosphorus ability of dissolving potassium; Powerful decomposition, conversion, synthetic effective part of supporting are improved utilization rate of fertilizer, improve the physicochemical character of soil; Promote crumb structure to form; Improve plant physiology, improve resistance and suppress various soil-borne diseases, repair and harden and ill soil; Unusual effects such as culture fertility improve crop yield and the final purpose that improves farmers' income thereby reach.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment with form or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall in protection scope of the present invention the details of technical scheme of the present invention.
The preparation of preparation embodiment 1 subtilis (Bacillus subtilis) microbial inoculum
Subtilis (Bacillus subtilis) is available from Chinese agriculture microbial strains preservation administrative center, goods number: ACCC01031;
1, Bacillus subtilis strain is inoculated in the substratum (the formulated substratum that provides by supplier) cultivates; With storing in 4 ℃ of refrigerators of medium slant seed bacterial strain; Take out activation twice before using, the bacterial strain after the activation is changeed 250 milliliters of eggplant bottle nutrient agar mediums cultivate, culture temperature is 37 ℃; Wash with aqua sterilisa during 48 hours well-growns, as the inoculum of seeding tank;
2, in 1 ton fermentor tank, add substratum, it consists of: Semen Maydis powder 3%, soybean cake powder 3%, bran powder 3%, K
2HPO
42.15%, KH
2PO
40.8%, MgSO
40.075%, peanut oil 0.3%.Initial pH7.5; To producing a jar inoculation, begin fermentation from seeding tank.Fermentation condition: culture temperature is 37 ℃, and ventilation is 1: 1.1, and tank pressure is 0.05mpa/cm
2, stirring velocity is 220rpm, inoculates after 20 hours and whenever takes a sample once at a distance from 4 hours; Observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach 90% or more after, the liquid fermenting completion; In zymocyte liquid, add conventional sorbent material and weighting agent, get bacillus subtilis microbial agent.
The preparation of preparation embodiment 2 Bacillus licheniformis (Bacillus subtilis) microbial inoculum
Bacillus licheniformis (Bacillus subtilis) is available from Chinese agriculture microbial strains preservation administrative center, goods number: ACCC01031;
1, the Bacillus licheniformis bacterial classification inoculation is cultivated in substratum (the formulated substratum that provides by supplier); With storing in 4 ℃ of refrigerators of medium slant seed bacterial strain; Take out activation twice before using, the bacterial strain after the activation is changeed 250 milliliters of eggplant bottle nutrient agar mediums cultivate, culture temperature is 37 ℃; Wash with aqua sterilisa during 48 hours well-growns, as the inoculum of seeding tank.
2, in 1 ton fermentor tank, add substratum, it consists of: Semen Maydis powder 3%, soybean cake powder 3%, bran powder 3%, K
2HPO
42.15%, KH
2PO
40.8%, MgSO
40.075%, peanut oil 0.3%.Initial pH7.5.To producing a jar inoculation, begin fermentation from seeding tank.Fermentation condition: culture temperature is 37 ℃, and ventilation is 1: 1.1, and tank pressure is 0.05mpa/cm
2, stirring velocity is 220rpm, inoculates after 20 hours and whenever takes a sample once at a distance from 4 hours; Observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach 90% or more after, the liquid fermenting completion; In zymocyte liquid, add conventional sorbent material and weighting agent, get bacillus licheniformis agent.
The preparation of preparation embodiment 3 S. cervisiaes (Saccharomyces cerevisiae) microbial inoculum
S. cervisiae (Saccharomyces cerevisiae) is available from Guangdong Microbes Inst culture presevation administrative center, goods number: GIM 2.39;
1, the S. cervisiae bacterial classification inoculation is cultivated in substratum (the formulated substratum that provides by supplier); Medium slant seed bacterial strain added behind the MO store in 4 ℃ of refrigerators, take out activation twice before using, the bacterial strain after the activation is changed in 250 milliliters of eggplant bottles cultivate; Culture temperature is 28-30 ℃; Cultivated 48 hours, and with sterile saline it was washed when strain growth is good, eluate is the inoculum of seeding tank.
2, in 1 ton fermentor tank, add substratum, wort is generally 10～15 ripple woodss, transfers pH to 6.4.To producing a jar inoculation, inoculum size is 50kg, begins fermentation from seeding tank.Fermentation condition: culture temperature is 28 ℃, and tank pressure is 0.05mpa/cm
2, stirring velocity is 220rpm, ventilating ratio 1: 1.1; Inoculate after 20 hours and whenever took a sample once, observe the thalli growth situation, whether pollution is arranged, after liquid fermenting is accomplished at a distance from 4 hours; In zymocyte liquid, add conventional sorbent material and weighting agent XG 550, get the yeast saccharomyces cerevisiae bacteria agent.
The preparation of preparation embodiment 4 Rhodopseudomonas palustriss (Rhodopseudomonas palustris) microbial inoculum
Rhodopseudomonas palustris (Rhodopseudomonas palustris) is available from Guangdong Microbes Inst culture presevation administrative center, goods number: GIM1.167;
1, the Rhodopseudomonas palustris bacterial classification inoculation is cultivated in substratum (the formulated substratum that provides by supplier); Medium slant seed bacterial strain added behind the MO store in 4 ℃ of refrigerators; Take out twice of activation before using; Change the bacterial strain after the activation over to 500 milliliters of vial illumination liquid culture, culture temperature is 30 ℃, cultivates 96 hours.Change 5L vial illumination cultivation 96 hours afterwards over to as secondary seed solution.
2, in 1 ton fermentor tank, add substratum, it consists of: 1.145 kilograms of sodium-acetates, 0.055 kilogram of peptone, 0.6 kilogram of sodium hydrogencarbonate, 0.4 kilogram in Sulfothiorine, 0.3 kilogram in sodium-chlor, 0.1 kilogram in sal epsom, 0.05 kilogram of potassium primary phosphate.With filtering 0.1N phosphoric acid adjust pH 7.0.Illumination 30001ux.To producing a jar inoculation, inoculum size is 50kg, begins fermentation from seeding tank.Fermentation condition: culture temperature is 30 ℃; Inoculate after 20 hours and whenever took a sample once, observe the thalli growth situation, whether pollution is arranged, after liquid fermenting is accomplished at a distance from 4 hours; In zymocyte liquid, add conventional sorbent material and weighting agent XG 550, get the Rhodopseudomonas palustris microbial inoculum.
The preparation of preparation embodiment 5 plant lactobacillus microbial inoculums
(1) plant lactobacillus bacterial classification (Lactobacillus plantarum) (available from Chinese agriculture microbial strains preservation administrative center, numbering ACCC11016) is inoculated in (Carnis Bovis seu Bubali cream 3 grams of cultivation in the beef tea peptone slant medium; Peptone 5～10 grams; The NaC15 gram is settled to 1000 milliliters with the dissolved in distilled water mentioned component, adjust pH to 7); Beef tea protein culture medium inclined-plane seed bacterial strain added behind the MO store in 4 ℃ of refrigerators, take out twice of activation before using.
(2) preparation solid spawn
1 part of peptone, 0.5 part of yeast powder, 0.2 part of ammonium citrate, 2 parts of glucose, 0.054 part in sal epsom; 1 part of Carnis Bovis seu Bubali cream, 0.2 part of potassium hydrogenphosphate, 0.5 part of sodium-acetate, 0.025 part of manganous sulfate, 1.5 parts in agar; 93.021 parts in water, natural pH value divides to be filled to test tube, sterilizes 20 minutes for 15 pounds.The streak inoculation lactobacterium plantarum strain, 37 ℃ of cultivations.
(3) preparation second class inoculum (liquid)
(1) culture medium prescription does not only add agar with the solid spawn substratum, natural pH value, and packing of 500ml saline bottle and tool plug were sterilized 20 minutes for 15 pounds, connect bacteria suspension 3～5% weight percents of above-mentioned solid spawn, 30～37 ℃ of constant temperature culture;
(2) in fermentor tank, add substratum, following ingredients is dissolved in the tap water of purification: glucose 5%, peptone 0.2%, yeast extract paste 0.2%, sal epsom 0.01%, manganous sulfate 0.01%, potassium hydrogenphosphate 0.01%, 0.2%, 116 ℃ of autoclaving of sodium-chlor; (3) inoculate to fermentor tank from seeding tank, inoculum size is 10%, begins fermentation; Fermentation condition: culture temperature is 37 ℃, and tank pressure is 0.05mpa/cm
2, regularly stirring, stirring velocity is 220rpm, pH reduces to fermentation in 3.5 o'clock and accomplishes, and gets the plant lactobacillus microbial inoculum.
The preparation of preparation embodiment 6 Lactobacterium acidophilum microbial inoculums
(1) with Lactobacterium acidophilum bacterial classification (Lactobacillus acidophilus) (available from available from Chinese common micro-organisms culture presevation administrative center; Bacterium numbering CGMCC1.1854) is inoculated in cultivation in the beef tea peptone slant medium (Carnis Bovis seu Bubali cream 3 grams, peptone 5～10 grams, NaCl 5 grams; Be settled to 1000 milliliters with the dissolved in distilled water mentioned component; Adjust pH to 7), beef tea protein culture medium inclined-plane seed bacterial strain added behind the MO stores in 4 ℃ of refrigerators, take out twice of activation before using.
(2) preparation solid spawn
1 part of peptone, 0.5 part of yeast powder, 0.2 part of ammonium citrate, 2 parts of glucose, 0.054 part in sal epsom; 1 part of Carnis Bovis seu Bubali cream, 0.2 part of potassium hydrogenphosphate, 0.5 part of sodium-acetate, 0.025 part of manganous sulfate, 1.5 parts in agar; 93.021 parts in water, natural pH value divides to be filled to test tube, sterilizes 20 minutes for 15 pounds.The streak inoculation lactobacterium plantarum strain, 37 ℃ of cultivations.
(3) preparation second class inoculum (liquid)
(1) culture medium prescription does not only add agar with the solid spawn substratum, natural pH value, and packing of 500ml saline bottle and tool plug were sterilized 20 minutes for 15 pounds, connect bacteria suspension 3～5% weight percents of above-mentioned solid spawn, 30～37 ℃ of constant temperature culture.；
(2) in fermentor tank, add substratum; Following ingredients is dissolved in the tap water of purification: glucose 5%; Peptone 0.2%, yeast extract paste 0.2%, sal epsom 0.01%, manganous sulfate 0.01%, potassium hydrogenphosphate 0.01%, sodium-chlor 0.2%, 116 autoclaving;
(3) inoculate to fermentor tank from seeding tank, inoculum size is 10%, begins fermentation; Fermentation condition: culture temperature is 37 ℃, and tank pressure is 0.05mpa/cm
2, regularly stirring, stirring velocity is 220rpm, pH reduces to fermentation in 3.5 o'clock and accomplishes, and gets the plant lactobacillus microbial inoculum.
Preparation embodiment 7 is adsorbed with the preparation of the organic mixture of complex microorganism liquid preparation
(1) preparation complex microorganism liquid preparation: plant lactobacillus microbial inoculum, Lactobacterium acidophilum microbial inoculum, water are mixed according to 40: 40: 20 part by weight, obtain the complex microorganism liquid preparation;
(2) the complex microorganism liquid preparation is adsorbed in zeolite powder, dregs of beans in the organic mixture that the pomace and the peat composed of rotten mosses are formed, promptly gets; Wherein, the weight part of each component is: 4 parts of complex microorganism liquid preparations, 60 parts of dregs of beans, 35 parts of pomaces, 45 parts of the peats composed of rotten mosses.
Preparation embodiment 8 is adsorbed with the preparation of the organic mixture of complex microorganism liquid preparation
(1) preparation complex microorganism liquid preparation: plant lactobacillus microbial inoculum, Lactobacterium acidophilum microbial inoculum, water are mixed according to 30: 50: 20 part by weight, obtain the complex microorganism liquid preparation;
(2) the complex microorganism liquid preparation is adsorbed in zeolite powder, dregs of beans in the organic mixture that the pomace and the peat composed of rotten mosses are formed, promptly gets; Wherein, the weight part of each component is: complex microorganism liquid preparation 2, dregs of beans 40, pomace 25, the peat composed of rotten mosses 30.
Preparation embodiment 9, be adsorbed with the preparation of the organic mixture of complex microorganism liquid preparation
(1) preparation complex microorganism liquid preparation: plant lactobacillus microbial inoculum, Lactobacterium acidophilum microbial inoculum, water are mixed according to 50: 30: 20 part by weight, obtain the complex microorganism liquid preparation;
(2) the complex microorganism liquid preparation is adsorbed in zeolite powder, dregs of beans in the organic mixture that the pomace and the peat composed of rotten mosses are formed, promptly gets; Wherein, the weight part of each component is: complex microorganism liquid preparation 3, dregs of beans 50, pomace 30, the peat composed of rotten mosses 40.
The preparation of embodiment 1 biological organic fertilizer
Take by weighing each raw material (unit: kg): the organic mixture that is adsorbed with the complex microorganism liquid preparation 10 that preparation embodiment 7 is prepared by following weight; Cottonseed meal 60, bacillus subtilis microbial agent 13, bacillus licheniformis agent 10; Yeast saccharomyces cerevisiae bacteria agent 11, Rhodopseudomonas palustris microbial inoculum 6;
Cottonseed meal pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The preparation of embodiment 2 biological organic fertilizers
Take by weighing each raw material (unit: kg): the organic mixture that is adsorbed with the complex microorganism liquid preparation 8 that preparation embodiment 8 is prepared by following weight; Soybean meal 40, bacillus subtilis microbial agent 10, bacillus licheniformis agent 10; Yeast saccharomyces cerevisiae bacteria agent 8, Rhodopseudomonas palustris microbial inoculum 5;
Cottonseed meal pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The preparation of embodiment 3 biological organic fertilizers
Take by weighing each raw material (unit: kg): the organic mixture that is adsorbed with the complex microorganism liquid preparation 9 that preparation embodiment 9 is prepared by following weight; Peanut meal 70, bacillus subtilis microbial agent 26, bacillus licheniformis agent 15; Yeast saccharomyces cerevisiae bacteria agent 12, Rhodopseudomonas palustris microbial inoculum 15;
Cottonseed meal pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The preparation of embodiment 4 biological organic fertilizers
Take by weighing each raw material (unit: kg): the organic mixture 8 that is adsorbed with the complex microorganism liquid preparation by following weight; The mixture 50 of cottonseed meal and rapeseed meal, bacillus subtilis microbial agent 18, bacillus licheniformis agent 12; Yeast saccharomyces cerevisiae bacteria agent 10, Rhodopseudomonas palustris microbial inoculum 12;
Cottonseed meal pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The preparation of embodiment 5 biological organic fertilizers
Take by weighing each raw material (unit: kg): the organic mixture 8.5 that is adsorbed with the complex microorganism liquid preparation by following weight; The pig manure of fermentation maturity (drying) 60, bacillus subtilis microbial agent 15, bacillus licheniformis agent 10; Yeast saccharomyces cerevisiae bacteria agent 12, Rhodopseudomonas palustris microbial inoculum 8;
Pig manure after drying pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The preparation of embodiment 6 biological organic fertilizers
Take by weighing each raw material (unit: kg): the organic mixture 9.5 that is adsorbed with the complex microorganism liquid preparation by following weight; The chicken manure of fermentation maturity (drying) 60, bacillus subtilis microbial agent 15, bacillus licheniformis agent 10; Yeast saccharomyces cerevisiae bacteria agent 12, Rhodopseudomonas palustris microbial inoculum 8;
Chicken manure after drying pulverized the back mixes with above-mentioned four kinds of microbial inoculums, dry, packing promptly gets.
The effect test of Test Example 1 biological organic fertilizer of the present invention on the paddy rice of reclamation area, Heilungkiang
One, test materials
1, supplies the prepared biological organic fertilizer of examination fertilizer: embodiment of the invention 1-6.
2, contrast fertilizer: Granted publication number be that CN 101642772B, denomination of invention are the biological organic fertilizer of " a kind of microbe soil restoration agent and preparation method thereof ".
3, trial crops: paddy rice.
Two, test place and time
Test place: more than 30 farm, reclamation area, Heilungkiang and R&D institution.
Test period: year September in April, 2006-2009.
Three, TP
Be rice soil experimental field, soil fertility is higher, is fit to the paddy rice plantation.Test is made as 6 treatment group and 1 control group:
Handle 1 group: use the prepared biological organic fertilizer of the embodiment of the invention 1;
Handle 2 groups: use the prepared biological organic fertilizer of the embodiment of the invention 2;
Handle 3 groups: use the prepared biological organic fertilizer of the embodiment of the invention 3;
Handle 4 groups: use the prepared biological organic fertilizer of the embodiment of the invention 4;
Handle 5 groups: use the prepared biological organic fertilizer of the embodiment of the invention 5;
Handle 6 groups: use the prepared biological organic fertilizer of the embodiment of the invention 6;
Control group: use Granted publication number biological organic fertilizer for " CN 101642772B ".
Each treatment group is provided with twice repetition.The accumulative total test area is 250,000 mu, 684.6 kilograms of average yield per mus.Be treated to 4 kilograms every mu biological organic fertilizer of the present invention and base manure and puddle and evenly be sprinkled into the rice field, soaked field then discharges water.Sowing mid-April, rice transplanting mid-May.
Four, result and analysis
Table 1 is the rice yield constituent element and the yield result of biological organic fertilizer of the present invention.Visible by table 1: compare with the contrast of not applying fertilizer, the paddy rice of biological organic fertilizer treatment group of the present invention is for improving paddy rice anti contravariance property, promoting to tiller that positive effect is arranged.Through the paddy rice that biological organic fertilizer of the present invention is handled, 4.7 of its leaf age average out to, contrast increases by 0.6 leaf age, root is long to be to increase 2cm than control group by 10cm, plant height contrast increase about 5cm and the leaf look dark green.
Table 1
Test-results shows that biological organic fertilizer of the present invention can significantly improve the output of crop, and effect of increasing production is obvious, wherein, handle 1 group particularly outstanding on the effect that improves rice yield than control group and other 5 treatment group.
The effect of Test Example 2 biological organic fertilizers of the present invention on Hunan Research Centre for Hybrid Rice paddy rice
Test
One, test materials
1, supplies the prepared biological organic fertilizer of examination fertilizer: embodiment of the invention 1-6.
2, contrast fertilizer: the biological organic fertilizer of using " Granted publication number for CN 101642772B ".
3, trial crops: paddy rice.
Two, test place and time
Test place: base, Huaihua Lu Yang town.
Test period: in May, 2010-2010 year September.
Three, TP
Experimental field soil property is glued earth, and soil fertility is medium.
Test design: test is divided into test group and control group; Test group is made as 6 treatment group and 1 control group;
Handle 1 group: use the embodiment of the invention 1 prepared biological organic fertilizer and add conventional fertilizer application;
Handle 2 groups: use the embodiment of the invention 2 prepared biological organic fertilizers and add conventional fertilizer application;
Handle 3 groups: use the embodiment of the invention 3 prepared biological organic fertilizers and add conventional fertilizer application;
Handle 4 groups: use the embodiment of the invention 4 prepared biological organic fertilizers and add conventional fertilizer application;
Handle 5 groups: use the embodiment of the invention 5 prepared biological organic fertilizers and add conventional fertilizer application;
Handle 6 groups: use the embodiment of the invention 6 prepared biological organic fertilizers and add conventional fertilizer application;
Control group: use contrast fertilizer and add conventional fertilizer application.
Described conventional fertilizer application applying method: for using sufficient base fertilizer, every mu execute carbon ammonium 30-40 kilogram before the arable land by conventional high yield fertilising, 50 kilograms in deep placement phosphate fertilizer, farmyard manure 50 loads, 1 kilogram of zinc fertilizer.Rice shoot tri-leaf period, every mu imposes urea 5-6 kilogram, and 40% composite fertilizer makes tillering fertilizer for 20 kilograms.See that after a week every mu of seedling imposes 5 kilograms of urea, potash fertilizer 5-6 kilogram is made balance fertilizer.Dry field ascites is fertile, and every mu chases after composite fertilizer's 5-10 kilogram of 40% and compares.Conventional fertilizer can be bought at Agricultural Materials shop.
Each treatment group is provided with twice repetition.
The plot, experimental plot is upright, and the area of each treatment group or control group is respectively 2.6 mu, and physical features is open, and is sunny.This experiment is chosen 3 shops at each Da Qu diagonal lines, and meter 20 root and stem of certain plants of whenever counting are looked into number of productive ear, ask its MV; Secondly, press every of MV picked at random 3 root and stem of certain plants appearance nearby.At last, the appearance of each point is counted separately and is carried out species test calculating fringe kernel structure.A species test treating water spike of rice, grain, setting percentage, thousand seed weight etc. are measured output, investigation and analysis output structure respectively during harvesting.
Four, test-results and analysis
Can find out that from table 2 using biological organic fertilizer of the present invention in paddy rice can effectively improve paddy rice thousand seed weight common fertilization method, every mu of actual output increases significantly.Wherein, handle 1 group particularly outstanding on the effect that improves paddy rice thousand seed weight and every mu of actual output than control group and other 5 treatment group.
Table 2
The effect test of Test Example 3 biological organic fertilizers of the present invention on Hunan Research Centre for Hybrid Rice paddy rice
One, test materials
1, supplies the prepared biological organic fertilizer of examination fertilizer: embodiment of the invention 1-6.
2, contrast fertilizer: use Granted publication number biological organic fertilizer for " CN 101642772B ".
3, trial crops: paddy rice.
Two, test place and time
Test place: breeding center, lichee ditch Hunan, Sanya, Hainan Province.
Test period: year July in January, 2010-2010.
Three, TP
This experiment soil type is the rice soil that oceanic sediment is grown, silty loam, and soil layer is shallow, arable layer 13cm; Soil test item: 1. organic content: 5.09 (2.10); 2. inorganic nutrients content M (0.17), P (0.12), K (0.15); 3.pH value: 6.5; 4. middle trace element: 0.001000049 (0.08); 5. useful microbe YY40%, harmful microorganism YH960%; 6. soil granular shape: ō-10 * 5.334 (2.50).
The experimental plot area is 0.78 mu, test kind " dark two excellent 5814 ";
Test is provided with treatment group and control group, and treatment group is used the prepared biological organic fertilizer of embodiment of the invention 1-6 and added conventional fertilizer application; The biological organic fertilizer that control group is used " Granted publication number for CN 101642772B " adds conventional fertilizer application.
Every sub-district area 60m
2, field, sub-district stalk is used film wrapped, with antiseep.Test-results sees table 3 and table 4 for details.
Table 3 biological organic fertilizer test seedling fringe record cartogram
Table 4 biological organic fertilizer test output tab structure
Four, test-results and analysis
Can find out that from table 3 and table 4 biological organic fertilizer of the present invention is applied in the cultivation of super hybridization rice performance better.Mainly show: it is fast to turn green after the transplanting, the field investigation theoretical yield, and every total grain panicle number, real grain number, setting percentage and thousand seed weight all have obvious increase than contrast, and actual output contrasts fertilizer has remarkable increase.
Claims (10)
1. biological organic fertilizer that improves crop anti-adversity and output comprises following component:
Be adsorbed with the organic mixture of complex microorganism liquid preparation, organic substance, subtilis (
Bacillus subtilis) microbial inoculum, Bacillus licheniformis (
Bacillus licheniformis) microbial inoculum, S. cervisiae (
Saccharomyces cerevisiae) microbial inoculum, Rhodopseudomonas palustris (
Rhodopseudomonas palustris) microbial inoculum, the plant lactobacillus agent (
Lactobacillus plantarum) and the Lactobacterium acidophilum agent (
Lactobacillus acidophilus).
2. according to the described biological organic fertilizer of claim 1, it is characterized in that the weight part of each component is: the organic mixture 8-10 part that is adsorbed with the complex microorganism liquid preparation; Organic substance 30-50 part; Bacillus subtilis microbial agent 10-20 part, bacillus licheniformis agent 7-12 part, yeast saccharomyces cerevisiae bacteria agent 7-12 part; Rhodopseudomonas palustris microbial inoculum 4-9 part, plant lactobacillus microbial inoculum 4-9 and Lactobacterium acidophilum microbial inoculum 4-9.
3. according to the described biological organic fertilizer of claim 2, it is characterized in that the weight part of each component is: 10 parts of organic mixtures that are adsorbed with the complex microorganism liquid preparation; 35 parts of organic substances, 15 parts of bacillus subtilis microbial agents, 8 parts of bacillus licheniformis agents; 8 parts of yeast saccharomyces cerevisiae bacteria agents, 5 parts of Rhodopseudomonas palustris microbial inoculums, 5 parts of plant lactobacillus microbial inoculums; 4 parts of Lactobacterium acidophilum microbial inoculums.
4. according to the described biological organic fertilizer of claim 1, it is characterized in that the described organic mixture that is adsorbed with the complex microorganism liquid preparation prepares according to following method:
(1) preparation complex microorganism liquid preparation: plant lactobacillus (Lactobacillus plantarum) microbial inoculum, Lactobacterium acidophilum (Lactobacillus acidophilus) microbial inoculum and the water part by weight according to 30-50:30-50:10-30 is mixed, promptly get; Preferably, thing probiotic lactobacillus microbial inoculum, Lactobacterium acidophilum microbial inoculum, water are mixed according to the part by weight of 40:40:20;
(2) the complex microorganism liquid preparation is adsorbed in the organic mixture, promptly gets the organic mixture that is adsorbed with the complex microorganism liquid preparation; Preferably, described organic mixture preferably is made up of dregs of beans, pomace, the peat composed of rotten mosses; In the described organic mixture that is adsorbed with the complex microorganism liquid preparation, the weight part of each component preferably: complex microorganism liquid preparation 2-4 part, dregs of beans 40-60 part, pomace 25-35 part, peat composed of rotten mosses 30-45 part.
5. according to the described biological organic fertilizer of claim 1, it is characterized in that: described organic substance is selected from any one or the multiple mixture of forming according to any mass ratio in the animal excrement of cottonseed meal, soybean meal, peanut meal, rapeseed meal or fermentation maturity.
6. according to the described biological organic fertilizer of claim 5, it is characterized in that: described animal excrement comprise pig manure, duck excrement, chicken manure or sheep excrement.
7. method for preparing any one described biological organic fertilizer of claim 1-6 may further comprise the steps: organism is pulverized back and the organic mixture that is adsorbed with the complex microorganism liquid preparation, subtilis (
Bacillus subtilis) microbial inoculum, Bacillus licheniformis (
Bacillus licheniformis) microbial inoculum, S. cervisiae
(Saccharomyces cerevisiae)Microbial inoculum, Rhodopseudomonas palustris
(Rhodopseudomonas palustris)Microbial inoculum, plant lactobacillus (
Lactobacillus plantarum) microbial inoculum, the Lactobacterium acidophilum microbial inoculum (
Lactobacillus acidophilus) mix, drying, promptly get.
8. any one described biological organic fertilizer of claim 1-6 is improving crop anti-adversity, promoting plant growth and is improving the application in the crop yield.
9. according to the described application of claim 8, it is characterized in that: described crop is a paddy rice.
10. according to the described application of claim 8, it is characterized in that: when paddy rice is planted, use it for the base manure of paddy rice or topdress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102749410A CN102432399B (en) | 2011-09-16 | 2011-09-16 | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102749410A CN102432399B (en) | 2011-09-16 | 2011-09-16 | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102432399A true CN102432399A (en) | 2012-05-02 |
| CN102432399B CN102432399B (en) | 2013-06-12 |
Family
ID=45980795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102749410A Active CN102432399B (en) | 2011-09-16 | 2011-09-16 | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102432399B (en) |
Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845270A (en) * | 2012-09-29 | 2013-01-02 | 重庆翠京元有机农业专业合作社 | Organic rice cultivation method |
| CN102965319A (en) * | 2012-11-30 | 2013-03-13 | 山东省林业科学研究院 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
| CN104402650A (en) * | 2014-10-29 | 2015-03-11 | 黑龙江盛瑞康生物科技开发有限公司 | Microbial inoculant powder for rice and corn plantation |
| CN106699252A (en) * | 2016-11-16 | 2017-05-24 | 江苏益客食品有限公司 | Biological organic fertilizer and preparation method and application thereof |
| CN106954639A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of preparation of complex microorganism bacterium solution and its application process |
| CN106954640A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of method of utilization microbial control trees disease |
| CN107047159A (en) * | 2017-03-01 | 2017-08-18 | 陈来凤 | A kind of method for culturing seedlings of paddy rice |
| CN107445711A (en) * | 2017-09-15 | 2017-12-08 | 黑龙江宝基农业科技股份有限公司 | Bio-organic fertilizer made using high calcium selenium-riched vegetable bud and preparation method thereof |
| CN108794173A (en) * | 2018-06-29 | 2018-11-13 | 锦州义隆经贸有限公司 | A kind of rice oil chaff biomass organic fertilizer |
| CN109896900A (en) * | 2019-04-26 | 2019-06-18 | 沈阳华生生物科技开发有限公司 | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer |
| CN110862278A (en) * | 2019-12-06 | 2020-03-06 | 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) | Facultative anaerobe biological fertilizer |
| CN111575203A (en) * | 2020-05-18 | 2020-08-25 | 上海净业农业科技发展有限公司 | Agricultural compound microbial agent and preparation method thereof |
| CN111705017A (en) * | 2020-06-24 | 2020-09-25 | 南通大学 | Bacterial manure preparation, preparation method and application thereof |
| CN112174718A (en) * | 2020-09-29 | 2021-01-05 | 东北农业大学 | Microbial preparation for promoting rooting and lodging resistance of rice and application thereof |
| CN112501052A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof |
| CN112500220A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112500216A (en) * | 2020-11-02 | 2021-03-16 | 东北农业大学 | Bio-organic fertilizer with crop rooting promoting function and preparation method thereof |
| CN112794749A (en) * | 2021-01-08 | 2021-05-14 | 东北农业大学 | Bio-organic fertilizer for improving yield and quality of potatoes and preparation method thereof |
| CN113248317A (en) * | 2021-06-10 | 2021-08-13 | 东北农业大学 | Preparation method of leaf fertilizer for promoting rice maturity and improving rice yield |
| CN113308382A (en) * | 2021-05-14 | 2021-08-27 | 海南大学 | Biocontrol bacterium preparation for enhancing stress resistance of crops |
| CN113749118A (en) * | 2021-08-06 | 2021-12-07 | 林猷宪 | Agricultural preparation with functions of killing insects, killing mites, sterilizing and resisting rice lodging |
| CN113979797A (en) * | 2021-07-19 | 2022-01-28 | 海南大学 | Microbial fermentation fertilizer for adjusting crop resistance |
| CN114231458A (en) * | 2021-12-24 | 2022-03-25 | 青岛尚德生物技术有限公司 | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent |
| CN115124389A (en) * | 2022-01-29 | 2022-09-30 | 哈尔滨皮加诺生物科技有限公司 | Preparation method of microbial agent composition for rice cultivation |
| CN116445371A (en) * | 2023-06-13 | 2023-07-18 | 青冈县龙农生物科技开发有限公司 | Microbial fermentation inoculant, and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322697A (en) * | 2000-05-10 | 2001-11-21 | 山东龙凤制造有限公司 | Preparation of multiple-biological organic and inorganic fertilizer |
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101429059A (en) * | 2008-12-11 | 2009-05-13 | 张庆国 | Green organic agronomic crop microorganism bacterial manure and method of producing the same |
| CN101642772A (en) * | 2009-08-26 | 2010-02-10 | 王颖 | Microbe soil restoration agent and preparation method thereof |
| CN101935236A (en) * | 2010-09-27 | 2011-01-05 | 上海创博生态工程有限公司 | Method for preparing acid organic fertilizer by orientation fermentation of organic waste |
-
2011
- 2011-09-16 CN CN2011102749410A patent/CN102432399B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322697A (en) * | 2000-05-10 | 2001-11-21 | 山东龙凤制造有限公司 | Preparation of multiple-biological organic and inorganic fertilizer |
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101429059A (en) * | 2008-12-11 | 2009-05-13 | 张庆国 | Green organic agronomic crop microorganism bacterial manure and method of producing the same |
| CN101642772A (en) * | 2009-08-26 | 2010-02-10 | 王颖 | Microbe soil restoration agent and preparation method thereof |
| CN101935236A (en) * | 2010-09-27 | 2011-01-05 | 上海创博生态工程有限公司 | Method for preparing acid organic fertilizer by orientation fermentation of organic waste |
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845270A (en) * | 2012-09-29 | 2013-01-02 | 重庆翠京元有机农业专业合作社 | Organic rice cultivation method |
| CN102965319A (en) * | 2012-11-30 | 2013-03-13 | 山东省林业科学研究院 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
| CN102965319B (en) * | 2012-11-30 | 2013-12-11 | 山东省林业科学研究院 | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof |
| CN104402650A (en) * | 2014-10-29 | 2015-03-11 | 黑龙江盛瑞康生物科技开发有限公司 | Microbial inoculant powder for rice and corn plantation |
| CN106954640A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of method of utilization microbial control trees disease |
| CN106954639A (en) * | 2016-01-08 | 2017-07-18 | 康源绿洲生物科技（北京）有限公司 | A kind of preparation of complex microorganism bacterium solution and its application process |
| CN106699252A (en) * | 2016-11-16 | 2017-05-24 | 江苏益客食品有限公司 | Biological organic fertilizer and preparation method and application thereof |
| CN107047159A (en) * | 2017-03-01 | 2017-08-18 | 陈来凤 | A kind of method for culturing seedlings of paddy rice |
| CN107445711A (en) * | 2017-09-15 | 2017-12-08 | 黑龙江宝基农业科技股份有限公司 | Bio-organic fertilizer made using high calcium selenium-riched vegetable bud and preparation method thereof |
| CN108794173A (en) * | 2018-06-29 | 2018-11-13 | 锦州义隆经贸有限公司 | A kind of rice oil chaff biomass organic fertilizer |
| CN109896900A (en) * | 2019-04-26 | 2019-06-18 | 沈阳华生生物科技开发有限公司 | A kind of preparation and application of complex micro organism fungicide and biological organic complex fertilizer |
| CN110862278A (en) * | 2019-12-06 | 2020-03-06 | 曲靖市土壤肥料工作站(曲靖市土壤肥料测试中心) | Facultative anaerobe biological fertilizer |
| CN111575203A (en) * | 2020-05-18 | 2020-08-25 | 上海净业农业科技发展有限公司 | Agricultural compound microbial agent and preparation method thereof |
| CN111705017A (en) * | 2020-06-24 | 2020-09-25 | 南通大学 | Bacterial manure preparation, preparation method and application thereof |
| CN112174718A (en) * | 2020-09-29 | 2021-01-05 | 东北农业大学 | Microbial preparation for promoting rooting and lodging resistance of rice and application thereof |
| CN112500216A (en) * | 2020-11-02 | 2021-03-16 | 东北农业大学 | Bio-organic fertilizer with crop rooting promoting function and preparation method thereof |
| CN112500220A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof |
| CN112501052A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof |
| CN112794749A (en) * | 2021-01-08 | 2021-05-14 | 东北农业大学 | Bio-organic fertilizer for improving yield and quality of potatoes and preparation method thereof |
| CN113308382A (en) * | 2021-05-14 | 2021-08-27 | 海南大学 | Biocontrol bacterium preparation for enhancing stress resistance of crops |
| CN113248317A (en) * | 2021-06-10 | 2021-08-13 | 东北农业大学 | Preparation method of leaf fertilizer for promoting rice maturity and improving rice yield |
| CN113979797A (en) * | 2021-07-19 | 2022-01-28 | 海南大学 | Microbial fermentation fertilizer for adjusting crop resistance |
| CN113749118A (en) * | 2021-08-06 | 2021-12-07 | 林猷宪 | Agricultural preparation with functions of killing insects, killing mites, sterilizing and resisting rice lodging |
| CN114231458A (en) * | 2021-12-24 | 2022-03-25 | 青岛尚德生物技术有限公司 | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent |
| CN115124389A (en) * | 2022-01-29 | 2022-09-30 | 哈尔滨皮加诺生物科技有限公司 | Preparation method of microbial agent composition for rice cultivation |
| CN115124389B (en) * | 2022-01-29 | 2023-10-20 | 哈尔滨皮加诺生物科技有限公司 | Preparation method of microbial agent composition for rice cultivation |
| CN116445371A (en) * | 2023-06-13 | 2023-07-18 | 青冈县龙农生物科技开发有限公司 | Microbial fermentation inoculant, and preparation method and application thereof |
| CN116445371B (en) * | 2023-06-13 | 2023-10-03 | 青冈县龙农生物科技开发有限公司 | Microbial fermentation inoculant, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102432399B (en) | 2013-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN103819237B (en) | Tobacco special microorganism is fertile and preparation method thereof | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN101168491B (en) | Microorganism soil repairing agent and its preparation method and application | |
| CN100572337C (en) | A kind of whole-fertilizer compound fertilizer and preparation method thereof | |
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN101863702B (en) | Special multifunctional fertilizer for peanut and preparation method thereof | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN104761379A (en) | Novel multifunctional compound microbial fertilizer and preparation method thereof | |
| CN105198672A (en) | Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof | |
| CN103113167A (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN102491802A (en) | Tea seed cake biological organic fertilizer and production method thereof | |
| CN101130465A (en) | Grain type humic acid compound biological fertilizer and manufacturing method therefor | |
| CN103449886A (en) | Composite microbial fertilizer for paddy | |
| CN102344812A (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN109180316A (en) | A kind of rice Special compound microbial fertilizer and preparation method thereof | |
| CN105777229A (en) | Organic fermented fertilizer for raising selenium content of vegetables, production method and cultivation method | |
| CN102786934A (en) | Probiotic soil conditioner and production method thereof | |
| CN106520131A (en) | Saline alkali soil improver and preparation method thereof | |
| CN106673866A (en) | Bacillus subtilis microbial fertilizer and preparation method thereof | |
| CN102731213A (en) | Fertilizer for cultivation of tobacco, preparation method and application thereof | |
| CN105198509A (en) | Special biological organic fertilizer for cotton and preparation method of special biological organic fertilizer | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240129 Address after: 071200 No.1 Central Street, Modern Traditional Chinese Medicine Industrial Park, Anguo City, Baoding City, Hebei Province Patentee after: Hebei Tongyuan Baicao Biotechnology Co.,Ltd. Country or region after: China Address before: 611, Yingu Building, No. 9 North Fourth Ring West Road, Haidian District, Beijing, 100080 Patentee before: BEIJING PINGANFU BIOLOG TECHNOLOGY RES INST Co.,Ltd. Country or region before: China |
|
| TR01 | Transfer of patent right |