CN113979797A - Microbial fermentation fertilizer for adjusting crop resistance - Google Patents
Microbial fermentation fertilizer for adjusting crop resistance Download PDFInfo
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- CN113979797A CN113979797A CN202110811961.0A CN202110811961A CN113979797A CN 113979797 A CN113979797 A CN 113979797A CN 202110811961 A CN202110811961 A CN 202110811961A CN 113979797 A CN113979797 A CN 113979797A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a microbial fermented fertilizer for regulating crop resistance, which consists of 0.05-0.1 wt% of astragalus polysaccharide and the balance of compost leavening, wherein the compost leavening is prepared by uniformly mixing bacterial fermentation liquor and fermentation substrate in a mass ratio of (4-7): 80-90, composting and fermenting for 9-12 days; the fermentation substrate comprises, by mass, 5-10 parts of angelica sinensis dregs, 5-10 parts of starwort roots dregs, 5-10 parts of pinellia ternate dregs, 30-50 parts of bean dregs, 30-50 parts of cottonseed meal and a proper amount of water; the bacterial fermentation liquid is a mixture of bacillus amyloliquefaciens fermentation liquid and rhodopseudomonas palustris fermentation liquid. The invention is prepared by adding astragalus polysaccharide into compost products such as traditional Chinese medicine residues and the like, and the active ingredients, strains and the astragalus polysaccharide in the traditional Chinese medicine residues can be fully exerted under the synergistic effect of all the components, so that the resistance of crops can be effectively adjusted, the growth of the crops can be promoted, and the yield of the crops can be improved.
Description
Technical Field
The invention relates to a microbial fermented fertilizer, in particular to a microbial fermented fertilizer for regulating crop resistance.
Background
The traditional Chinese medicine industry is one of the traditional Chinese industries, and a great amount of Chinese patent medicines are produced every year and a considerable amount of Chinese medicine residues are also produced. If the traditional Chinese medicine residues are randomly stacked, the environment can be polluted, especially in rainy season, the traditional Chinese medicine residues are flushed by rainwater, and the serious pollution is caused to underground water and surface water. On one hand, the herb residue is discarded as waste, and on the other hand, the herb residue usually contains abundant primary biomass such as protein, sugar, cellulose, mineral substances, etc. Because the existing traditional Chinese medicine extraction method and process are relatively single, part of medicinal components in the traditional Chinese medicine are still remained in the medicine dregs, and the residual primary and secondary substances enable the medicine dregs to still have high recycling value.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a microbial fermentation fertilizer for regulating crop resistance, which is prepared by composting traditional Chinese medicine residues and the like, can fully play the role of active ingredients in the traditional Chinese medicine residues, effectively regulates the crop resistance, promotes the crop growth and improves the crop yield.
The invention provides a microbial fermented fertilizer for regulating crop resistance, which consists of 0.05-0.1 wt% of astragalus polysaccharide and the balance of compost leavening, wherein the compost leavening is prepared by uniformly mixing bacterial fermentation liquor and fermentation substrate in a mass ratio of (4-7): 80-90, and performing compost fermentation for 9-12 days; the fermentation substrate comprises, by mass, 5-10 parts of angelica sinensis dregs, 5-10 parts of starwort roots dregs, 5-10 parts of pinellia ternate dregs, 30-50 parts of bean dregs, 30-50 parts of cottonseed meal and a proper amount of water; the bacterial fermentation liquid is a mixture of bacillus amyloliquefaciens fermentation liquid and rhodopseudomonas palustris fermentation liquid.
Preferably, the water content of the fermentation substrate is 40-50%.
Preferably, the total viable count of the bacterial fermentation liquor is more than or equal to 3 multiplied by 107cfu/ml, and the ratio of viable count of Bacillus amyloliquefaciens to viable count of Rhodopseudomonas palustris is (1-10): 1-10.
Further preferably, the ratio of the number of viable bacteria of the bacillus amyloliquefaciens and the rhodopseudomonas palustris in the bacterial fermentation liquid is (3-7): 5-8.
The culture medium for fermentation culture is not particularly limited herein, and the bacillus amyloliquefaciens may be any culture medium as long as it can perform fermentation culture on the bacillus amyloliquefaciens, and the rhodopseudomonas palustris may be any culture medium as long as it can perform fermentation culture on the bacillus licheniformis, and those skilled in the art can select the culture medium according to the strain property.
The second aspect of the present invention provides a method for preparing a microbial fermented fertilizer according to the first aspect of the present invention, comprising the following steps: (1) respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to culture solution for liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation solution and rhodopseudomonas palustris fermentation solution, and then mixing to obtain bacterial fermentation solution; mixing radix Angelicae sinensis residue, radix Stellariae residue, rhizoma Pinelliae residue, bean dregs, cotton seed meal and water to obtain fermentation substrate; (2) uniformly mixing the bacterial fermentation liquor and the fermentation substrate, and performing compost fermentation for 9-12 days to obtain compost fermentation products; (3) and uniformly mixing the compost fermentation product with astragalus polysaccharide to obtain the composition.
The culture medium for seed culture is not particularly limited herein, and the Bacillus amyloliquefaciens may be any culture medium as long as it can culture seeds of Bacillus amyloliquefaciens, and the Rhodopseudomonas palustris may be any culture medium as long as it can culture seeds of Bacillus licheniformis, and those skilled in the art can select the culture medium according to the nature of the strain.
The culture medium for fermentation culture is not particularly limited herein, and the bacillus amyloliquefaciens may be any culture medium as long as it can perform fermentation culture on the bacillus amyloliquefaciens, and the rhodopseudomonas palustris may be any culture medium as long as it can perform fermentation culture on the bacillus licheniformis, and those skilled in the art can select the culture medium according to the strain property.
The third aspect of the invention provides the application of the microbial fermented fertilizer of the first aspect of the invention in regulating crop resistance, promoting crop growth and improving crop yield.
Preferably, the microbial fermentation fertilizer is used as a base fertilizer when crops are planted.
When the microbial fermented fertilizer is used, the microbial fermented fertilizer can be used independently or together with other fertilizers, the base fertilizer is turned over and cultivated after the surface of the land is spread, and the microbial fermented fertilizer can also be used as the base fertilizer for furrow application during sowing, wherein the using amount of the microbial fermented fertilizer per mu is 7-8 kg.
The active ingredients of the microbial fermentation fertilizer comprise bacillus amyloliquefaciens and fermentation metabolites thereof, rhodopseudomonas palustris and fermentation metabolites thereof, astragalus polysaccharides, products of Chinese angelica dregs fermented and metabolized by the bacillus amyloliquefaciens and the rhodopseudomonas palustris, products of starwort dregs fermented and metabolized by the bacillus amyloliquefaciens and the rhodopseudomonas palustris, products of pinellia ternate dregs fermented and metabolized by the bacillus amyloliquefaciens and the rhodopseudomonas palustris and the like, and the active ingredients belong to different action mechanisms.
Detailed Description
The invention will be better understood by reference to the following examples. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
In the present invention, the culture medium for seed culture is not particularly limited, and the bacillus amyloliquefaciens may be any culture medium capable of seed culture of bacillus amyloliquefaciens, or rhodopseudomonas palustris may be any culture medium capable of seed culture of bacillus licheniformis, and those skilled in the art can select the culture medium according to the nature of the strain.
In the specific embodiment of the present invention, the culture medium for fermentation culture is not particularly limited, and the bacillus amyloliquefaciens may be any culture medium as long as it can perform fermentation culture on the bacillus amyloliquefaciens, and the rhodopseudomonas palustris may be any culture medium as long as it can perform fermentation culture on the bacillus licheniformis, and those skilled in the art can select the culture medium according to the strain property.
Example 1
Respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to the culture solution to carry out liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation liquor and rhodopseudomonas palustris fermentation liquor, and then mixing to obtain bacterial fermentation liquor, wherein the viable count of the bacillus amyloliquefaciens in the bacterial fermentation liquor is 3 multiplied by 107cfu/ml, viable count of Rhodopseudomonas palustris 8X 107cfu/ml。
According to the mass parts, 10 parts of angelica sinensis dregs, 10 parts of starwort roots dregs, 10 parts of pinellia ternate dregs, 30 parts of bean dregs and 50 parts of cottonseed meal are taken, a proper amount of water is added and mixed uniformly to obtain a fermentation substrate, and the water content of the fermentation substrate is controlled to be within a range of 40-50%.
And (4) taking 4kg of bacterial fermentation liquor and 90kg of fermentation substrate, uniformly mixing, and carrying out compost fermentation for 9d to obtain compost fermentation product. And then adding astragalus polysaccharide into the compost fermentation product, and uniformly mixing to obtain the microbial fermentation fertilizer, wherein the content of the astragalus polysaccharide in the microbial fermentation fertilizer is 0.1 wt%.
Example 2
Respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to the culture solution to carry out liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation liquor and rhodopseudomonas palustris fermentation liquor, and then mixing to obtain bacterial fermentation liquor, wherein the viable count of the bacillus amyloliquefaciens in the bacterial fermentation liquor is 7 multiplied by 107cfu/ml, viable count of Rhodopseudomonas palustris 5X 107cfu/ml。
According to the mass parts, 5 parts of angelica sinensis dregs, 10 parts of starwort roots dregs, 5 parts of pinellia ternate dregs, 50 parts of bean dregs and 30 parts of cottonseed meal are added with a proper amount of water and mixed uniformly to obtain a fermentation substrate, and the water content of the fermentation substrate is controlled to be within a range of 40-50%. And (3) taking 7kg of bacterial fermentation liquor and 80kg of fermentation substrate, uniformly mixing, and carrying out compost fermentation for 12d to obtain compost fermentation product. And then adding astragalus polysaccharide into the compost fermentation product, and uniformly mixing to obtain the microbial fermentation fertilizer, wherein the content of the astragalus polysaccharide in the microbial fermentation fertilizer is 0.05 wt%.
Example 3
Respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to the culture solution to carry out liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation liquor and rhodopseudomonas palustris fermentation liquor, and then mixing to obtain bacterial fermentation liquor, wherein the viable count of the bacillus amyloliquefaciens in the bacterial fermentation liquor is 1 multiplied by 107cfu/ml, viable count of Rhodopseudomonas palustris 1X 108cfu/ml。
According to the mass parts, 10 parts of angelica sinensis dregs, 5 parts of starwort roots dregs, 5 parts of pinellia ternate dregs, 50 parts of bean dregs and 50 parts of cottonseed meal are taken, a proper amount of water is added and mixed uniformly to obtain a fermentation substrate, and the water content of the fermentation substrate is controlled to be 40-50%.
And (3) taking 5kg of bacterial fermentation liquor and 85kg of fermentation substrate, uniformly mixing, and carrying out compost fermentation for 10d to obtain compost fermentation product. And then adding astragalus polysaccharide into the compost fermentation product, and uniformly mixing to obtain the microbial fermentation fertilizer, wherein the content of the astragalus polysaccharide in the microbial fermentation fertilizer is 0.08 wt%.
Example 4
Respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to the culture solution to carry out liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation liquor and rhodopseudomonas palustris fermentation liquor, and then mixing to obtain bacterial fermentation liquor, wherein the viable count of the bacillus amyloliquefaciens in the bacterial fermentation liquor is 1 multiplied by 108cfu/ml, viable count of Rhodopseudomonas palustris 1X 107cfu/ml。
According to the mass parts, 10 parts of angelica sinensis dregs, 10 parts of starwort roots dregs, 5 parts of pinellia ternate dregs, 30 parts of bean dregs and 30 parts of cottonseed meal are taken, a proper amount of water is added and mixed uniformly to obtain a fermentation substrate, and the water content of the fermentation substrate is controlled to be within a range of 40-50%.
And (3) taking 7kg of bacterial fermentation liquor and 90kg of fermentation substrate, uniformly mixing, and carrying out compost fermentation for 11d to obtain compost fermentation product. And then adding astragalus polysaccharide into the compost fermentation product, and uniformly mixing to obtain the microbial fermentation fertilizer, wherein the content of the astragalus polysaccharide in the microbial fermentation fertilizer is 0.1 wt%.
Comparative example 1
Essentially the same as example 1, except that no astragalus polysaccharide was added.
Comparative example 2
Essentially the same as in example 1, except that the bacterial broth was a bacillus amyloliquefaciens broth, and no rhodopseudomonas palustris broth was added, i.e., no rhodopseudomonas palustris was included.
Comparative example 3
Essentially the same as in example 1, except that the bacterial broth was a rhodopseudomonas palustris broth, with no addition of rhodopseudomonas palustris broth, i.e., no bacillus amyloliquefaciens.
Comparative example 4
The same as example 1, except that no angelica sinensis residue was added to the fermentation substrate.
Comparative example 5
The same as example 1, except that the fermentation substrate was not added with bupleurum scorzonerifolium dregs.
Comparative example 6
The same as example 1, except that no pinellia ternate residue was added to the fermentation substrate.
Test example 1: application effect test of microbial fermentation fertilizer on rice
The same batch of rice 'Shenliangyou 5814' is adopted to be cultivated in rice soil developed by ocean sediments in Hainan, 1 mu of land is cultivated in each embodiment/comparative example, the microbial fermented fertilizer prepared in the embodiment 1 is adopted in the experimental group, the land surface is spread according to the use amount of 8 kg/mu, then the cultivation base fertilizer is turned over, the microbial fermented fertilizer prepared in the comparative examples 1-6 is adopted in the comparative group, the land surface is spread according to the use amount of 8 kg/mu, then the cultivation base fertilizer is turned over, the blank group adopts clear water to replace the base fertilizer, and then the cultivation is carried out according to the conventional cultivation method of the rice. Specific data are shown in table 1 (averaged).
TABLE 1
| Thousand Kernel weight (g) | Yield (kg/mu) | |
| Experimental group 1 | 31.52 | 712.3 |
| Control group 1 | 26.98 | 658.1 |
| Control group 2 | 27.53 | 663.9 |
| Control group 3 | 27.28 | 681.0 |
| Control group 4 | 27.14 | 671.4 |
| Control group 5 | 27.66 | 675.2 |
| Control group 6 | 27.43 | 669.4 |
| Blank group | 26.54 | 634.3 |
Test results show that the microbial fermentation fertilizer can obviously improve the resistance of crops, promote the growth of the crops and improve the yield of the crops.
The results of the same tests using the microbial fermentation fertilizers of examples 2-4 were similar to those of example 1.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (7)
1. A microbial fermented fertilizer for regulating crop resistance is characterized by comprising 0.05-0.1 wt% of astragalus polysaccharide and the balance of compost leavening, wherein the compost leavening is prepared by uniformly mixing bacterial fermentation liquor and fermentation substrate in a mass ratio of (4-7): 80-90, composting and fermenting for 9-12 days;
the fermentation substrate comprises, by mass, 5-10 parts of angelica sinensis dregs, 5-10 parts of starwort roots dregs, 5-10 parts of pinellia ternate dregs, 30-50 parts of bean dregs, 30-50 parts of cottonseed meal and a proper amount of water;
the bacterial fermentation liquid is a mixture of bacillus amyloliquefaciens fermentation liquid and rhodopseudomonas palustris fermentation liquid.
2. The microbial fermented fertilizer according to claim 1, wherein the water content of the fermentation substrate is 40-50%.
3. The microbial fermented fertilizer according to claim 1, wherein the total viable count of the bacterial fermented liquid is not less than 3 x 107cfu/ml, and Bacillus amyloliquefaciens and swamp redThe ratio of viable count of Pseudomonas is (1-10): 1-10.
4. The microbial fermented fertilizer according to claim 3, wherein the ratio of viable bacteria number of the bacillus amyloliquefaciens and the rhodopseudomonas palustris in the bacterial fermented liquid is (3-7): 5-8.
5. A method for preparing a microbial fermented fertilizer according to any one of claims 1-4, comprising the steps of:
(1) respectively inoculating bacillus amyloliquefaciens and rhodopseudomonas palustris to culture solution for liquid seed culture and liquid fermentation culture to obtain bacillus amyloliquefaciens fermentation solution and rhodopseudomonas palustris fermentation solution, and then mixing to obtain bacterial fermentation solution;
mixing radix Angelicae sinensis residue, radix Stellariae residue, rhizoma Pinelliae residue, bean dregs, cotton seed meal and water to obtain fermentation substrate;
(2) uniformly mixing the bacterial fermentation liquor and the fermentation substrate, and performing compost fermentation for 9-12 days to obtain compost fermentation products;
(3) and uniformly mixing the compost fermentation product with astragalus polysaccharide to obtain the composition.
6. Use of the microbial fermented fertilizer of any one of claims 1-4 for regulating crop resistance, promoting crop growth and increasing crop yield.
7. The use according to claim 6, wherein the microbial fermentation fertilizer is used as a base fertilizer at the time of crop planting.
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Cited By (1)
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| CN116769662A (en) * | 2023-06-16 | 2023-09-19 | 云南省农业科学院农业环境资源研究所 | Pseudomonas putida, microbial inoculum and biological preparation and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116769662A (en) * | 2023-06-16 | 2023-09-19 | 云南省农业科学院农业环境资源研究所 | Pseudomonas putida, microbial inoculum and biological preparation and application thereof |
| CN116769662B (en) * | 2023-06-16 | 2024-01-23 | 云南省农业科学院农业环境资源研究所 | Pseudomonas putida, microbial inoculum and biological preparation and application thereof |
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