CN112501052A - Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof - Google Patents
Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof Download PDFInfo
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Abstract
The invention discloses a compound microbial inoculum for improving saline-alkali soil and application thereof. The raw materials comprise the following components in parts by weight: 5-10 parts of composite microbial inoculum, 1-10 parts of brown sugar, 10-20 parts of urea, 60-84 parts of soluble starch and 1000 parts of clear water 800-. The compound microorganism bacterium liquid and the chemical fertilizer are applied in a matching way, when the chemical fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid and the chemical fertilizer are uniformly mixed and then applied to the soil in a drip irrigation or flood irrigation mode, and the application amount of the compound microorganism bacterium liquid is 75-300kg/hm each time2. The invention can reduce the salt content and pH value of the saline-alkali soil, promote the growth of crops, improve the yield of the crops and improve the soil structure and ecological environment of the saline-alkali soil.
Description
Technical Field
The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a compound microbial liquid for improving saline-alkali soil and application thereof.
Background
With the development of economy and the progress of technology, the problems of continuous decrease of farmland resources, deterioration of farmland quality and slow increase of agricultural yield in agriculture in China are increasingly prominent. However, the saline soil and alkaline earth area of China is about 3-4 hundred million acres, which is equivalent to one fifth of the existing cultivated land of China, so that the great development and utilization of the improved saline-alkali soil have great significance for increasing the cultivated land area of China and the sustainable development of agriculture.
At present, biological improvement in the saline-alkali soil improvement method has the characteristics of high salt rejection rate, durability and stability, and microorganisms are applied to saline-alkali soil to improve the micro-ecological environment of the soil, so that the soil property of the saline-alkali soil is improved, and the method has important significance for improving the saline-alkali soil.
Disclosure of Invention
The invention aims to provide a compound microbial liquid for improving saline-alkali soil and application thereof, and the compound microbial liquid can reduce the salt content and pH value of the saline-alkali soil.
On one hand, the invention provides a compound microbial inoculum for improving saline-alkali soil, which is prepared from raw materials consisting of a compound microbial inoculum, brown sugar, urea, soluble starch and clear water.
Preferably, the complex microorganism liquid is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation at the temperature of 22-28 ℃ for 48 hours.
Preferably, the raw materials comprise, by weight, 5-10 parts of the complex microbial inoculum, 1-10 parts of brown sugar, 10-20 parts of urea, 60-84 parts of soluble starch and 1000 parts of clean water 800-.
Preferably, the raw materials comprise, by weight, 5 parts of the complex microbial inoculum, 3 parts of brown sugar, 10 parts of urea, 67 parts of soluble starch and 800 parts of clear water.
Preferably, the raw materials comprise 7 parts of the complex microbial inoculum, 1 part of brown sugar, 14 parts of urea, 60 parts of soluble starch and 870 parts of clear water by weight.
Preferably, the raw materials comprise 8 parts of the complex microbial inoculum, 10 parts of brown sugar, 17 parts of urea, 78 parts of soluble starch and 930 parts of clear water by weight.
Preferably, the raw materials comprise, by weight, 10 parts of the complex microbial inoculum, 7 parts of brown sugar, 20 parts of urea, 84 parts of soluble starch and 1000 parts of clean water.
Preferably, the complex microbial inoculum comprises aspergillus glaucus, cyanobacteria flavipes, polyporus roseus, lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis.
Preference is given toThe bacteria content of the aspergillus glaucus is more than or equal to 4.0 multiplied by 109cfu/g, the bacterial content of the yellow blue shaped bacteria is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 5.0 multiplied by 109cfu/g, the bacterium content of the bacillus subtilis is more than or equal to 2.0 multiplied by 1010cfu/g。
On the other hand, the invention also provides the application of the compound microorganism bacterium liquid, when a chemical fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid and the chemical fertilizer are uniformly mixed, then the mixture is dissolved in clear water and then applied to soil, and the application amount of the compound microorganism bacterium liquid is 75-300 kg/hm/every time2。
The compound microorganism bacterium liquid can reduce the salt content and the pH value of the saline-alkali soil, promote the growth of crops, improve the yield of the crops and improve the soil structure and the ecological environment of the saline-alkali soil.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention mixes brown sugar, urea, soluble starch and clear water and adopts beneficial microorganisms to ferment and prepare the compound microorganism bacterial liquid for improving saline-alkali soil. The bacterial liquid can reduce the salt content and pH value of saline-alkali soil, promote the growth of crops, increase the yield of crops and improve the soil structure and ecological environment of the saline-alkali soil.
In a specific embodiment of the invention, the compound microbial inoculum for improving the saline-alkali soil is prepared from raw materials consisting of a compound microbial inoculum, brown sugar, urea, soluble starch and clear water. The specific preparation process comprises the following steps: step 1, preparing a complex microbial inoculum; step 2, preparing a mixed material; and 3, fermenting the mixed material.
In the step 1 of preparing the composite microbial agent, Aspergillus glaucens (Aspergillus glauceus) powder, Talaromyces flavus (Talaromyces flavus) powder, Trichosporon roseum (Trichosporon roseum) powder, Lactobacillus plantarum (Lactobacillus plantarum) powder, Saccharomyces cerevisiae (Saccharomyces cerevisiae) powder and Bacillus subtilis powder are selected, and then the six bacteria powders are compounded according to the proportioning relationship of the weight to obtain the composite microbial agent, preferably 10-20 parts of the Aspergillus glaucens powder, 5-15 parts of the Aspergillus flavus powder, 10-20 parts of the polyporus pinus roseus powder, 15-25 parts of the Lactobacillus plantarum powder, 10-20 parts of the Saccharomyces cerevisiae powder and 15-25 parts of the Bacillus subtilis powder. On one hand, lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then thalli are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the content of Lactobacillus plantarum powder is more than or equal to 1.0 × 1011cfu/g, the bacteria content of the saccharomyces cerevisiae powder is more than or equal to 5.0 multiplied by 109cfu/g and the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g. On the other hand, the aspergillus glaucus, the rhizopus lutescens and the polyporus pinkistrotus are respectively fermented in corresponding solid culture media to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the bacteria content of the Aspergillus glaucus powder is not less than 4.0 × 109cfu/g, the bacterial content of the yellow blue shaped bacteria powder is more than or equal to 1.0 multiplied by 109The bacterium content of cfu/g and the powder of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g。
In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, and then all the raw materials are mixed and stirred uniformly. The weight ratio of each component in the raw materials is respectively optimized as follows: 5-10 parts of composite microbial inoculum, 1-10 parts of brown sugar, 10-20 parts of urea, 60-84 parts of soluble starch and 1000 parts of clear water 800-.
In the step 3 of fermenting the mixed material, the initial pH of the mixed material is natural, and then the mixed material is subjected to facultative fermentation for 48 hours at the temperature of 22-28 ℃, and the fermented product is used as the compound microorganism bacterial solution.
The invention also provides application of the compound microorganism bacterium liquid, and the compound microorganism bacterium liquid is applied together with a chemical fertilizer when improving saline-alkali soil. When the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid and the fertilizer are uniformly mixed, then dissolved in clear water and applied to soil, and the compound microorganism bacterium liquid is preferably applied to the soil in a drip irrigation or flood irrigation mode. The application amount of the compound microorganism liquid is preferably 75-300kg/hm2When the solid compound microbial foliar fertilizer is preferably continuously applied for more than 3 years, the solid compound microbial foliar fertilizer has better effect on improving medium and mild saline-alkali soil.
To help better understand the technical scheme of the present invention, the following examples are provided to illustrate the preparation process and application method of the complex microbial liquid of the present invention.
Example one
The raw materials of the compound microbial inoculum of the embodiment comprise, by weight, 5 parts of compound microbial inoculum, 3 parts of brown sugar, 10 parts of urea, 67 parts of soluble starch and 800 parts of clear water. The composite microbial agent is prepared by mixing 10 parts of aspergillus glaucus powder, 15 parts of bluish yellow fungus powder, 13 parts of pinkish polyporus fungus powder, 25 parts of plant lactobacillus fungus powder, 14 parts of saccharomyces cerevisiae powder and 15 parts of bacillus subtilis powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of the Aspergillus glaucus powder is not less than 4.0 × 109cfu/g, the bacterial content of the yellow blue shaped bacteria powder is more than or equal to 1.0 multiplied by 109The bacterium content of cfu/g and the powder of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterial content of the lactobacillus plantarum bacterial powder is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae powder is more than or equal to 5.0 multiplied by 109cfu/g and the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g。
The compound microbial liquid is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, albedonia fuscosporium powder, pinkish polyporus powder, lactobacillus plantarum powder, saccharomyces cerevisiae powder and bacillus subtilis powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, urea, soluble starch and clear water together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking the fermented product as the compound microbial liquid 1.
The application method of the compound microorganism bacterium liquid 1 comprises the following steps: when the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid 1 and the fertilizer are uniformly mixed, then dissolved in clear water, and applied to the soil in a drip irrigation mode. The application amount of the compound microorganism liquid 1 is 75kg/hm2。
Example two
The raw materials of the compound microbial inoculum of the embodiment comprise 7 parts of compound microbial inoculum, 1 part of brown sugar, 14 parts of urea, 60 parts of soluble starch and 870 parts of clear water by weight. The composite microbial agent is prepared by mixing 13 parts of aspergillus glaucus powder, 11 parts of bluish yellow fungus powder, 10 parts of pinkish polyporus frondosus powder, 19 parts of lactobacillus plantarum fungus powder, 17 parts of saccharomyces cerevisiae fungus powder and 18 parts of bacillus subtilis powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of the Aspergillus glaucus powder is not less than 4.0 × 109cfu/g, the bacterial content of the yellow blue shaped bacteria powder is more than or equal to 1.0 multiplied by 109The bacterium content of cfu/g and the powder of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterial content of the lactobacillus plantarum bacterial powder is more than or equal to 1.0 multiplied by 1011cfu/g, content of saccharomyces cerevisiae powderThe bacterial amount is not less than 5.0 × 109cfu/g and the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g。
The compound microbial liquid is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, albedonia fuscosporium powder, pinkish polyporus powder, lactobacillus plantarum powder, saccharomyces cerevisiae powder and bacillus subtilis powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, urea, soluble starch and clear water together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking the fermented product as the compound microorganism bacterium liquid 2.
The application method of the compound microorganism bacterium liquid 2 comprises the following steps: when the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid 2 and the fertilizer are uniformly mixed, then dissolved in clear water, and applied to the soil in a drip irrigation mode. The application amount of the compound microorganism liquid 2 is 150kg/hm2。
EXAMPLE III
The raw materials of the compound microbial inoculum of the embodiment comprise, by weight, 8 parts of compound microbial inoculum, 10 parts of brown sugar, 17 parts of urea, 78 parts of soluble starch and 930 parts of clear water. The composite microbial agent is prepared by mixing 17 parts of aspergillus glaucus powder, 8 parts of bluish yellow fungus powder, 16 parts of pinkish polyporus frondosus powder, 15 parts of plant lactobacillus fungus powder, 20 parts of saccharomyces cerevisiae powder and 25 parts of bacillus subtilis powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of the Aspergillus glaucus powder is not less than 4.0 × 109cfu/g, the bacterial content of the yellow blue shaped bacteria powder is more than or equal to 1.0 multiplied by 109The bacterium content of cfu/g and the powder of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterial content of the lactobacillus plantarum bacterial powder is more than or equal to 1.0 multiplied by 1011cfu/g, brewingThe content of wine yeast powder is not less than 5.0 × 109cfu/g and the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g。
The compound microbial liquid is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, albedonia fuscosporium powder, pinkish polyporus powder, lactobacillus plantarum powder, saccharomyces cerevisiae powder and bacillus subtilis powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, urea, soluble starch and clear water together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking the fermented product as the compound microbial liquid 3.
The application method of the compound microorganism bacterium liquid 3 comprises the following steps: when the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid 3 and the fertilizer are uniformly mixed, then dissolved in clear water, and applied to the soil in a drip irrigation mode. The application amount of the compound microorganism liquid 3 is 225kg/hm2。
Example four
The raw materials of the compound microbial inoculum of the embodiment comprise, by weight, 10 parts of compound microbial inoculum, 7 parts of brown sugar, 20 parts of urea, 84 parts of soluble starch and 1000 parts of clear water. The composite microbial agent is prepared by mixing 20 parts of aspergillus glaucus powder, 5 parts of bluish yellow fungus powder, 20 parts of pinkish polyporus fungus powder, 22 parts of plant lactobacillus fungus powder, 10 parts of saccharomyces cerevisiae powder and 21 parts of bacillus subtilis powder. The bacteria content of the six bacteria powder is respectively as follows: the bacteria content of the Aspergillus glaucus powder is not less than 4.0 × 109cfu/g, the bacterial content of the yellow blue shaped bacteria powder is more than or equal to 1.0 multiplied by 109The bacterium content of cfu/g and the powder of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterial content of the lactobacillus plantarum bacterial powder is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae powder is more than or equal to 5.0 multiplied by 109cfu/g and the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g。
The compound microbial liquid is prepared by the following steps.
Step 1, preparing a composite microbial inoculum, and uniformly mixing aspergillus glaucus powder, albedonia fuscosporium powder, pinkish polyporus powder, lactobacillus plantarum powder, saccharomyces cerevisiae powder and bacillus subtilis powder to obtain the composite microbial inoculum.
And 2, mixing the raw materials to prepare a mixed material, adding the composite microbial inoculum, brown sugar, urea, soluble starch and clear water together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃, and taking the fermented product as the compound microbial liquid 4.
The application method of the compound microorganism bacterium liquid 4 comprises the following steps: when the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid 4 and the fertilizer are uniformly mixed, then dissolved in clear water, and applied to the soil in a drip irrigation mode. The application amount of the compound microorganism liquid 4 is 300kg/hm2。
In order to help better understand the technical scheme of the invention, a test example of rice planting is provided below for illustrating the application effect of the invention.
The first test example: effect of compound microorganism bacterium liquid on saline-alkali soil improvement and influence on rice growth
The same saline-alkali field is selected, the basic physicochemical property of field soil is pH value 8.87, the total salt content of the soil of a plough layer is 8.10g/kg, the alkalization degree is 30.24%, the organic matter content is 16.82g/kg, the total nitrogen content is 1.05g/kg, the quick-acting phosphorus content is 20.03mg/kg, the quick-acting potassium content is 185mg/kg, and the alkaline hydrolysis nitrogen content is 32.10 mg/kg. 5 groups of experimental designs, including 4 experimental groups and 1 control group, each group of experimental designs 3 experimental cells, each area of 30m2All test cells are randomly distributed.
The test group is applied with fertilizer and the compound microorganism bacterium liquid prepared by the invention. In particular toThe fertilizer application mode is that urea, calcium superphosphate and potassium sulfate are used as base fertilizers to be applied to soil, and the application amount of the three fertilizers is 135kg/hm of urea respectively2Calcium superphosphate 90kg/hm2And potassium sulfate 90kg/hm2. Applying urea once at seedling stage, tillering stage and jointing stage of rice, wherein the urea applying amount is 22.5kg/hm245kg/hm at tillering stage2And the jointing-pulling booting period is 22.5kg/hm2. The application mode of the urea is that the urea is dissolved in clear water and then applied to soil in a drip irrigation mode. The compound microbial inoculum is applied for 4 times and respectively used as a base fertilizer, a rice seedling stage additional fertilizer, a rice tillering stage additional fertilizer and a rice jointing and booting stage additional fertilizer. When the compound microbial inoculum is applied each time, the compound microbial inoculum is uniformly mixed with urea, then dissolved in clear water and applied to soil in a drip irrigation mode. In 4 test groups, the application amount of the compound microorganism liquid 1 is 75kg/hm2The application amount of the compound microorganism liquid 2 is 150kg/hm2The application amount of the compound microbial liquid 3 is 225kg/hm2The application amount of the compound microorganism liquid 4 is 300kg/hm2. The control group does not apply the compound microorganism bacterial liquid, and the fertilizer application mode is the same as that of the test group.
Donggao No. 4 is selected as a test variety according to 60kg/hm in middle ten days of 4 months2Sowing the seeds at the sowing rate, performing the same conventional management on each district, and harvesting in the last 9 th of month. After harvesting the rice, the yield of each cell is counted, and the average cell yield of each group is calculated. And simultaneously, taking a soil sample to measure the pH, the total salt content and the alkalization degree of the soil in each cell, and calculating the average pH, the average total salt content and the average alkalization degree of each group of soil. The results are shown in Table 1.
TABLE 1
As can be seen from the data in Table 1, the pH, total salt content and alkalization degree of the rice planting soil of the 4 groups applied with the compound microbial inoculum are all obviously lower than those of the control, and the rice yield of the cell is obviously higher than that of the control. Therefore, the prepared compound microbial bacteria solution 1-compound microbial bacteria solution 4 can obviously improve saline-alkali soil, reduce the pH value and the salt content of the soil, promote the growth of rice and improve the yield of the rice.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (10)
1. A compound microorganism bacterium solution for improving saline-alkali soil is prepared from a compound bacterium agent, brown sugar, urea, soluble starch and clear water.
2. The complex microbial liquid of claim 1, wherein: the compound microbial liquid is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation for 48 hours at the temperature of 22-28 ℃.
3. The complex microbial liquid of claim 1, wherein: the raw materials comprise, by weight, 5-10 parts of the complex microbial inoculum, 1-10 parts of brown sugar, 10-20 parts of urea, 60-84 parts of soluble starch and 1000 parts of clean water.
4. The complex microbial broth of claim 3, wherein: the raw materials comprise, by weight, 5 parts of the complex microbial inoculum, 3 parts of brown sugar, 10 parts of urea, 67 parts of soluble starch and 800 parts of clear water.
5. The complex microbial broth of claim 3, wherein: the raw materials comprise 7 parts of the compound microbial inoculum, 1 part of brown sugar, 14 parts of urea, 60 parts of soluble starch and 870 parts of clear water by weight.
6. The complex microbial broth of claim 3, wherein: the raw materials comprise 8 parts of the compound microbial inoculum, 10 parts of brown sugar, 17 parts of urea, 78 parts of soluble starch and 930 parts of clear water by weight.
7. The complex microbial broth of claim 3, wherein: the raw materials comprise, by weight, 10 parts of the complex microbial inoculum, 7 parts of brown sugar, 20 parts of urea, 84 parts of soluble starch and 1000 parts of clear water.
8. The complex microbial broth of any one of claims 1 to 7, wherein: the compound microbial inoculum comprises aspergillus glaucus, ceruloplasmin, stenotrophospora roseoflavus, lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis.
9. The complex microbial broth of claim 8, wherein: the bacteria content of the aspergillus glaucus is more than or equal to 4.0 multiplied by 109cfu/g, the bacterial content of the yellow blue shaped bacteria is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 3.0 multiplied by 109cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.0 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 5.0 multiplied by 109cfu/g, the bacterium content of the bacillus subtilis is more than or equal to 2.0 multiplied by 1010cfu/g。
10. Use of the complex microbial broth of any one of claims 1 to 9, characterized in that: when the fertilizer is applied to crops planted in saline-alkali soil each time, the compound microorganism bacterium liquid and the fertilizer are uniformly mixed, then dissolved in clear water and applied to soil, and the application amount of the compound microorganism bacterium liquid is 75-300kg/hm2。
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