CN112586511A - Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method - Google Patents
Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/34—Aspergillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
Abstract
The invention discloses a seed dressing agent for promoting the germination of seeds of crops planted in saline-alkali soil and a seed treatment method. The seed treatment method of the present invention comprises: step 1, firstly diluting the seed dressing agent with clear water, and then uniformly spraying the diluted seed dressing agent on the surface of seeds; step 2, scattering an inert porous material on the surface of the seeds, and then uniformly mixing the seeds and the inert porous material; and 3, exposing the seeds for 2-3 hours and then sowing. The seed dressing agent can improve the seed germination rate of crops planted in saline-alkali soil, promote the growth of the crops and improve the yield of the crops.
Description
Technical Field
The invention belongs to the technical field of saline-alkali soil improvement, and particularly relates to a seed dressing agent for promoting the germination of crop seeds planted in saline-alkali soil and a seed treatment method.
Background
Saline-alkali soil is a general term for saline soil and alkaline earth. The saline-alkali soil is high in salinity, plants lose good soil environment and nutrition conditions by changing the physical and chemical properties of the soil, and the normal absorption and metabolism functions of the crops are influenced by directly damaging crop cells through the soil solution.
The biological improvement of saline-alkali soil is mainly to improve the physical and chemical properties of soil and the microclimate of soil by introducing, screening and planting salt-tolerant plants, thereby achieving the purposes of reducing the evaporation of soil moisture and inhibiting the salt return of soil.
The beneficial microorganisms can generate a large amount of physiological active substances and nutritional ingredients required by crops in life activities, and the physiological active substances and the nutritional ingredients can promote the germination of crop seeds and make the crops obtain the nutrients required by growth, thereby promoting the growth of the crops and improving the product quality of the crops and the ecological environment thereof.
Therefore, the seed dressing agent containing beneficial microorganisms is developed and applied to crops planted in saline-alkali soil, so that the growth of the crops is promoted, and the seed dressing agent has important significance for improving the saline-alkali soil.
Disclosure of Invention
The invention aims to provide a seed dressing agent for promoting the germination of seeds of crops planted in saline-alkali soil and a seed treatment method, so that the germination rate of the seeds of the crops planted in the saline-alkali soil is improved, and the growth of the crops is promoted.
In one aspect, the invention provides a seed dressing agent for promoting seed germination of crops planted in saline-alkali soil, which comprises aspergillus glaucus, verticillium flavum, stenotrophospora roseum, trichoderma harzianum, verticillium chlamydosporium, rhodospirillum rubrum, lactobacillus plantarum, saccharomyces cerevisiae and bacillus megaterium.
Preferably, the seed dressing agent comprises 1-10 parts of the aspergillus glaucus, 5-15 parts of the verticillium chrysosporium, 1-10 parts of the polyporus pinus, 10-20 parts of the trichoderma harzianum, 5-10 parts of the verticillium chlamydosporium, 10-20 parts of the rhodospirillum rubrum, 5-15 parts of the lactobacillus plantarum, 1-10 parts of the saccharomyces cerevisiae and 5-15 parts of the bacillus megaterium by weight.
Preferably, the seed dressing agent comprises 1 part by weight of aspergillus glaucus, 15 parts by weight of ceruloplasmin, 6 parts by weight of stenotrophospora rosea, 10 parts by weight of trichoderma harzianum, 5 parts by weight of verticillium chlamydosporia, 20 parts by weight of rhodospirillum rubrum, 9 parts by weight of lactobacillus plantarum, 7 parts by weight of saccharomyces cerevisiae and 11 parts by weight of bacillus megaterium.
Preferably, the seed dressing agent comprises 7 parts by weight of aspergillus glaucus, 11 parts by weight of ceruloplasmin, 10 parts by weight of stenotrophosporium roseum, 16 parts by weight of trichoderma harzianum, 8 parts by weight of verticillium chlamydosporium, 14 parts by weight of rhodospirillum rubrum, 15 parts by weight of lactobacillus plantarum, 10 parts by weight of saccharomyces cerevisiae and 5 parts by weight of bacillus megaterium.
Preferably, the seed dressing agent comprises, by weight, 10 parts of aspergillus glaucus, 5 parts of albuterococcus, 1 part of stenotrophospora rosea, 20 parts of trichoderma harzianum, 10 parts of verticillium chlamydosporium, 10 parts of rhodospirillum rubrum, 5 parts of lactobacillus plantarum, 1 part of saccharomyces cerevisiae, and 15 parts of bacillus megaterium.
In another aspect, the present invention also provides a method for treating seeds, comprising the steps of:
step 1, firstly diluting the seed dressing agent by using clear water, and then uniformly spraying the diluted seed dressing agent on the surface of seeds;
step 2, throwing an inert porous material to the surface of the seeds, and then uniformly mixing the seeds and the inert porous material;
and 3, exposing the seeds for 2-3 hours and then sowing.
Preferably, the dilution factor of the seed dressing agent is 5-10 times.
Preferably, the weight ratio of the seed dressing agent to the seed is 1: 500.
Preferably, the weight ratio of the inert porous material to the seeds is from 1:500 to 2: 500.
Preferably, the inert porous material is at least one of ceramic powder, zeolite powder or charcoal powder.
The seed dressing agent can improve the seed germination rate of crops planted in saline-alkali soil, promote the growth of the crops and improve the yield of the crops.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention relates to a seed dressing agent for promoting germination of crop seeds, which is prepared by mixing Aspergillus glauces (Aspergillus glauces) powder, Talaromyces flavus (Talaromyces flavus) powder, Trichosporon roseum (Trichoderma roseum) powder, Trichoderma harzianum (Trichoderma harzianum) powder, Verticillium chlamydosporium (Verticillum) powder, Rhodospirillum rubrum (Rhodospirillum rubrum) powder, Lactobacillus plantarum (Lactobacillus plantarum) powder, Saccharomyces cerevisiae (Saccharomyces cerevisiae) powder and Bacillus megaterium (Bacillus megatherium) powder according to a certain proportion and then mixing. The seed dressing agent can improve the seed germination rate of crops planted in saline-alkali soil, promote the growth of the crops and improve the yield of the crops.
The seed dressing agent preferably comprises nine kinds of bacterial powder in the following proportion by weight: 1-10 parts of aspergillus glaucus powder, 5-15 parts of bluish yellow fungus powder, 1-10 parts of polyporus pinus powder, 10-20 parts of trichoderma harzianum powder,5-10 parts of verticillium chlamydosporium powder, 10-20 parts of rhodospirillum rubrum powder, 5-15 parts of lactobacillus plantarum powder, 1-10 parts of saccharomyces cerevisiae powder and 5-15 parts of bacillus megaterium powder. Nine bacterial powders can be prepared by self, on one hand, rhodospirillum rubrum, lactobacillus plantarum, saccharomyces cerevisiae and bacillus megaterium are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids respectively, and then the bacteria are separated from the fermented culture and dried and concentrated to prepare single-strain solid bacterial powders. The bacterial content of each single bacterial strain powder is respectively as follows: the content of Rhodospirillum rubrum is not less than 1.0 × 1010cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.5 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 3.0 multiplied by 109cfu/g, the bacteria content of the bacillus megaterium is more than or equal to 2.0 multiplied by 1010cfu/g. On the other hand, aspergillus glaucus, rhizoctonia rosea, trichoderma harzianum and verticillium chlamydosporium are fermented in corresponding solid culture media to obtain solid cultures, and then the solid cultures are dried and crushed to prepare single-strain solid bacterial powder. The bacterial content of each single bacterial strain powder is respectively as follows: the bacteria content of Aspergillus glaucus is not less than 5.0 × 109cfu/g, the bacterial content of the yellow blue fungus is more than or equal to 1.5 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 2.5 multiplied by 109cfu/g, the bacterial content of Trichoderma harzianum is more than or equal to 4.5 multiplied by 109cfu/g, the bacterial content of Verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g。
In the process of saline-alkali soil improvement, seeds can be treated according to the following method before sowing:
step 1, firstly diluting the seed dressing agent by using clear water, and then uniformly spraying the diluted seed dressing agent on the surface of seeds;
step 2, throwing an inert porous material to the surface of the seeds, and then uniformly mixing the seeds and the porous material;
and 3, exposing the seeds for 2-3 hours and then sowing.
In the method, the dilution factor of the seed-dressing agent is preferably 5-10 times, the weight ratio of the seed-dressing agent to the seeds is preferably 1:500, and the weight ratio of the inert porous material to the seeds is preferably 1:500-2:500, wherein the inert porous material is preferably any one or combination of two or more of ceramic powder, zeolite powder or charcoal powder.
To assist in a better understanding of the technical solution of the present invention, the following examples are provided to illustrate the preparation process of the seed dressing agent and the treatment method of the seed according to the present invention.
Example one
The seed dressing agent comprises, by weight, 1 part of aspergillus glaucus powder, 15 parts of albedonia powder, 6 parts of pinkish polyporus roseus powder, 10 parts of trichoderma harzianum powder, 5 parts of verticillium chlamydosporium powder, 20 parts of rhodospirillum rubrum powder, 9 parts of lactobacillus plantarum powder, 7 parts of saccharomyces cerevisiae powder and 11 parts of bacillus megaterium powder. The bacterial contents of the nine bacterial powders are respectively as follows: the bacteria content of Aspergillus glaucus is not less than 5.0 × 109cfu/g, the bacterial content of the yellow blue fungus is more than or equal to 1.5 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 2.5 multiplied by 109cfu/g, the bacterial content of Trichoderma harzianum is more than or equal to 4.5 multiplied by 109cfu/g, the bacterial content of Verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of Rhodospirillum rubrum is more than or equal to 1.0 × 1010cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.5 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 3.0 multiplied by 109cfu/g, the bacteria content of the bacillus megaterium is more than or equal to 2.0 multiplied by 1010cfu/g。
The seed dressing agent is prepared by the following steps.
Step 1, weighing raw materials of the bacterial powder, and weighing aspergillus glaucus bacterial powder, verticillium flavum bacterial powder, pinkish polyporus bacterial powder, trichoderma harzianum bacterial powder, verticillium chlamydosporium bacterial powder, rhodospirillum rubrum bacterial powder, lactobacillus plantarum bacterial powder, saccharomyces cerevisiae bacterial powder and bacillus megaterium bacterial powder respectively according to a proportion relation.
And 2, mixing the raw material components, adding the bacterial powder weighed in the step 1 together, stirring and uniformly mixing to obtain the seed dressing agent 1.
The seed treatment method 1 comprises the following steps:
1) according to the amount of 1kg of seed dressing agent used for every 500kg of seeds, firstly diluting the seed dressing agent 1 by 5 times by using clear water, and then uniformly spraying the diluted seed dressing agent 1 on the surfaces of the seeds;
2) then according to the amount of 1kg of inert porous material used for every 500kg of seeds, ceramic powder is scattered on the surfaces of the seeds, and the seeds and the ceramic powder are mixed and stirred uniformly;
3) and (3) exposing the seeds for 2 hours, and then sowing the seeds in the saline-alkali soil.
Example two
The seed dressing agent comprises, by weight, 7 parts of aspergillus glaucus powder, 11 parts of albedonia powder, 10 parts of pinkish polyporus roseus powder, 16 parts of trichoderma harzianum powder, 8 parts of verticillium chlamydosporium powder, 14 parts of rhodospirillum rubrum powder, 15 parts of lactobacillus plantarum powder, 10 parts of saccharomyces cerevisiae powder and 5 parts of bacillus megaterium powder. The bacterial contents of the nine bacterial powders are respectively as follows: the bacteria content of Aspergillus glaucus is not less than 5.0 × 109cfu/g, the bacterial content of the yellow blue fungus is more than or equal to 1.5 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 2.5 multiplied by 109cfu/g, the bacterial content of Trichoderma harzianum is more than or equal to 4.5 multiplied by 109cfu/g, the bacterial content of Verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of Rhodospirillum rubrum is more than or equal to 1.0 × 1010cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.5 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 3.0 multiplied by 109cfu/g, the bacteria content of the bacillus megaterium is more than or equal to 2.0 multiplied by 1010cfu/g。
The seed dressing agent is prepared by the following steps.
Step 1, weighing raw materials of the bacterial powder, and weighing aspergillus glaucus bacterial powder, verticillium flavum bacterial powder, pinkish polyporus bacterial powder, trichoderma harzianum bacterial powder, verticillium chlamydosporium bacterial powder, rhodospirillum rubrum bacterial powder, lactobacillus plantarum bacterial powder, saccharomyces cerevisiae bacterial powder and bacillus megaterium bacterial powder respectively according to a proportion relation.
And 2, mixing the raw material components, adding the bacterial powder weighed in the step 1 together, stirring and uniformly mixing to obtain the seed dressing agent 2.
The seed treatment method 2 comprises the following steps:
1) according to the amount of 1kg of seed dressing agent used for every 500kg of seeds, firstly diluting the seed dressing agent 2 by 7 times by using clear water, and then uniformly spraying the diluted seed dressing agent 2 on the surfaces of the seeds;
2) then, according to the amount of 1.4kg of inert porous material used for every 500kg of seeds, zeolite powder is scattered on the surfaces of the seeds, and the seeds and the zeolite powder are mixed and stirred uniformly;
3) seeds are exposed for 2.5 hours and then sowed in saline-alkali soil.
EXAMPLE III
The seed dressing agent comprises, by weight, 10 parts of aspergillus glaucus powder, 5 parts of albedonia powder, 1 part of pinkish polyporus pinus powder, 20 parts of trichoderma harzianum powder, 10 parts of verticillium chlamydosporium powder, 10 parts of rhodospirillum rubrum powder, 5 parts of lactobacillus plantarum powder, 1 part of saccharomyces cerevisiae powder and 15 parts of bacillus megaterium powder. The bacterial contents of the nine bacterial powders are respectively as follows: the bacteria content of Aspergillus glaucus is not less than 5.0 × 109cfu/g, the bacterial content of the yellow blue fungus is more than or equal to 1.5 multiplied by 109cfu/g, the bacterium content of the polyporus pinus is more than or equal to 2.5 multiplied by 109cfu/g, the bacterial content of Trichoderma harzianum is more than or equal to 4.5 multiplied by 109cfu/g, the bacterial content of Verticillium chlamydosporium is more than or equal to 1.0 multiplied by 109cfu/g, the bacterium content of Rhodospirillum rubrum is more than or equal to 1.0 × 1010cfu/g, the bacterium content of the lactobacillus plantarum is more than or equal to 1.5 multiplied by 1011cfu/g, the bacteria content of the saccharomyces cerevisiae is more than or equal to 3.0 multiplied by 109cfu/g, the bacteria content of the bacillus megaterium is more than or equal to 2.0 multiplied by 1010cfu/g。
The seed dressing agent is prepared by the following steps.
Step 1, weighing raw materials of the bacterial powder, and weighing aspergillus glaucus bacterial powder, verticillium flavum bacterial powder, pinkish polyporus bacterial powder, trichoderma harzianum bacterial powder, verticillium chlamydosporium bacterial powder, rhodospirillum rubrum bacterial powder, lactobacillus plantarum bacterial powder, saccharomyces cerevisiae bacterial powder and bacillus megaterium bacterial powder respectively according to a proportion relation.
And 2, mixing the raw material components, adding the bacterial powder weighed in the step 1 together, stirring and uniformly mixing to obtain the seed dressing agent 3.
The seed treatment method 3 comprises the following steps:
1) according to the amount of 1kg of seed dressing agent used for every 500kg of seeds, firstly diluting the seed dressing agent 3 by 10 times with clear water, and then uniformly spraying the diluted seed dressing agent 3 on the surfaces of the seeds;
2) then, according to the amount of 2.0kg of inert porous material used for every 500kg of seeds, ceramic powder, zeolite powder and charcoal powder are scattered on the surfaces of the seeds, and the seeds and the three kinds of porous material powder are uniformly mixed, wherein the weight ratio of the ceramic powder to the zeolite powder to the charcoal powder is 1:1: 1;
3) and (3) exposing the seeds for 3 hours, and then sowing the seeds in the saline-alkali soil.
In order to help better understand the technical scheme of the invention, the following provides a test example of rice germination and rice planting for illustrating the application effect of the invention.
The first test example: influence of seed dressing agent on rice seed germination under salt stress
Selecting Changbai No. 9 as a test variety, weighing 144g of seeds, randomly dividing the seeds into 4 groups including 3 test groups and 1 control group, and dressing the rice seeds of the test groups with a seed dressing agent in a specific treatment mode: respectively weighing 0.07g of each of the seed-dressing agent 1-the seed-dressing agent 3, and then respectively adding 3 groups of the seed-dressing agents into clear water for dilution, wherein the seed-dressing agent 1 is diluted by 5 times, the seed-dressing agent 2 is diluted by 7 times, and the seed-dressing agent 3 is diluted by 10 times. Then, the diluted seed dressing agents of the groups are sprayed on the surfaces of the seeds of each test group, and the seeds are uniformly mixed and dried in a shade. The control group replaced the seed dressing agent with an equal amount of clear water and the remaining treatments were identical to the test groups.
300 seeds are randomly selected from each group of treated seeds, are evenly divided into 3 parts and are respectively sowed in 3 germination boxes, and 5ml of NaCl solution with the concentration of 50mmol/L is added into each germination box. The germination test was carried out in a 25 ℃ incubator, and the germination condition and the number of the germinated seeds were observed and recorded every day, and the germination standard was that the bud length was equal to half the seed length or the root length. After 7 days, the germination rate was calculated, and after 10 days, 15 pellets were randomly taken from each treatment, and the root length and number thereof were measured. The average germination percentage, average root length and average number of roots of each group were calculated. The results are shown in Table 1.
TABLE 1
As can be seen from the data in Table 1, the germination rate, root length and root number of the rice seeds of the 3 groups to which the seed dressing agent was applied were significantly higher than those of the control. Therefore, under the condition of salt stress, the prepared seed dressing agent 1-seed dressing agent 3 can obviously improve the germination rate of rice seeds and promote the germination of the rice seeds.
Test example two: influence of seed dressing agent on growth of rice planted in saline-alkali soil
The method is characterized in that the same saline-alkali field block is selected in the white city of Jilin province, the basic physicochemical property of field soil is that the pH value is 9.59, the total salt content of the soil of a plough layer is 1.88%, the organic matter content is 0.65%, the total nitrogen content is 0.31g/kg, the quick-acting phosphorus content is 9.25mg/kg, the quick-acting potassium content is 110mg/kg, and the alkaline hydrolysis nitrogen content is 18.45 mg/kg. 4 groups of experimental designs, including 3 experimental groups and 1 control group, each group of experimental designs 3 experimental cells, each area of the experimental cells is 30m2All test cells are randomly distributed.
The test groups applied fertilizers and seed dressings prepared according to the invention. The specific fertilization mode is that urea, calcium superphosphate and potassium sulfate are used as base fertilizers to be applied to soil, and the fertilization amounts of the three fertilizers are 320kg/hm of urea respectively2Calcium superphosphate 330kg/hm2And potassium sulfate 130kg/hm2. Respectively applying urea once in tillering stage and joint-pulling and booting stage of rice, wherein urea application amount is 150kg/hm2And 50kg/hm2. The specific application mode of the 3 seed dressings is as follows: according to the amount of 1kg of seed-dressing agent used for every 500kg of seeds, firstly diluting the seed-dressing agent by using clear water, then uniformly spraying the diluted seed-dressing agent on the surface of the seeds, then throwing inert porous material on the surface of the seeds, uniformly mixing the seeds and the inert porous material, then exposing and drying the seeds, and sowing the seeds. Wherein the seed dressing agent is diluted by 1 time by 5 times, the ceramic powder is applied according to the amount of 1.0kg of porous material applied to every 500kg of seeds, and the seeds are exposed for 2 hours; 2, diluting the seed dressing agent by 7 times, applying zeolite powder according to the amount of 1.4kg of porous material matched with each 500kg of seeds, and exposing the seeds for 2.5 hours; the seed dressing agent 3 was diluted 10-fold and applied in an amount of 2.0kg of the porous material per 500kg of seedsThe mixture of ceramic powder, zeolite powder and charcoal powder, the weight ratio of the three porous materials is 1:1:1, and the seeds are exposed for 3 hours. The control group was not applied with seed dressing, and the fertilizer was applied in the same manner as the test group.
Selecting Changbai No. 9 as test variety, and measuring the length of the test variety in the middle ten days of 4 months according to 65kg/hm2Sowing the seeds at the sowing rate, performing the same conventional management on each district, and harvesting in the last 9 th of month. The emergence rate of each plot was investigated at 15d of rice seeding. The seedling rate of each cell was investigated at the 3-leaf stage of rice, and at the same time, 10 seedlings were pulled from each cell, and the plant height, root number, and stem base width of the seedlings were investigated. After harvesting the rice, the yield of each cell was counted. And calculating the average rate of emergence, the average seedling rate, the average plant height, the average root number, the average stem base width and the average cell rice yield of each group. The results are shown in Table 2.
TABLE 2
As can be seen from the data in Table 2, the emergence rate, seedling rate, plant height, root number, stem base width and plot rice yield of the 3 groups of rice plants to which the seed dressing agent was applied were significantly higher than the control. Therefore, the prepared seed dressing agent 1-seed dressing agent 3 can obviously promote the germination of rice seeds and the growth of rice plants, and improve the yield of rice.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (10)
1. A seed dressing agent for promoting the germination of seeds of crops planted in saline-alkali soil comprises aspergillus glaucus, stramenopile, stenocarpella rosea, trichoderma harzianum, verticillium chlamydosporium, rhodospirillum rubrum, lactobacillus plantarum, saccharomyces cerevisiae and bacillus megaterium.
2. The seed dressing agent according to claim 1, characterized in that: the seed dressing agent comprises 1-10 parts of aspergillus glaucus, 5-15 parts of cyanobacteria, 1-10 parts of stenotrophospora rosea, 10-20 parts of trichoderma harzianum, 5-10 parts of verticillium chlamydosporium, 10-20 parts of rhodospirillum rubrum, 5-15 parts of lactobacillus plantarum, 1-10 parts of saccharomyces cerevisiae and 5-15 parts of bacillus megaterium by weight.
3. The seed dressing agent according to claim 2, characterized in that: the seed dressing agent comprises 1 part of aspergillus glaucus, 15 parts of albolabris, 6 parts of trichoderma roseum, 10 parts of trichoderma harzianum, 5 parts of verticillium chlamydosporium, 20 parts of rhodospirillum rubrum, 9 parts of lactobacillus plantarum, 7 parts of saccharomyces cerevisiae and 11 parts of bacillus megaterium by weight.
4. The seed dressing agent according to claim 2, characterized in that: the seed dressing agent comprises 7 parts of aspergillus glaucus, 11 parts of albuginea, 10 parts of stenotrophospora rosea, 16 parts of trichoderma harzianum, 8 parts of verticillium chlamydosporium, 14 parts of rhodospirillum rubrum, 15 parts of lactobacillus plantarum, 10 parts of saccharomyces cerevisiae and 5 parts of bacillus megaterium by weight.
5. The seed dressing agent according to claim 2, characterized in that: the seed dressing agent comprises 10 parts of aspergillus glaucus, 5 parts of albuterol, 1 part of trichoderma roseum, 20 parts of trichoderma harzianum, 10 parts of verticillium chlamydosporium, 10 parts of rhodospirillum rubrum, 5 parts of lactobacillus plantarum, 1 part of saccharomyces cerevisiae and 15 parts of bacillus megaterium by weight.
6. A method of treating seeds, comprising: the method comprises the following steps:
step 1, firstly diluting the seed dressing agent of any one of claims 1 to 5 by using clear water, and then uniformly spraying the diluted seed dressing agent on the surface of seeds;
step 2, throwing an inert porous material to the surface of the seeds, and then uniformly mixing the seeds and the inert porous material;
and 3, exposing the seeds for 2-3 hours and then sowing.
7. The method of claim 6, wherein: the dilution times of the seed dressing agent are 5-10 times.
8. The method of claim 6, wherein: the weight ratio of the seed dressing agent to the seeds is 1: 500.
9. The method of claim 6, wherein: the weight ratio of the inert porous material to the seeds is 1:500-2: 500.
10. Method for the treatment of seeds according to any one of claims 6 to 9, characterized in that: the inert porous material is at least one of ceramic powder, zeolite powder or charcoal powder.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349223A (en) * | 2021-07-01 | 2021-09-07 | 黑龙江佳星农业科技开发有限公司 | Plant microorganism seed dressing agent and use method thereof |
Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5985795A (en) * | 1996-10-04 | 1999-11-16 | Akio Suganuma | Antimicrobial composition |
| CN101974428A (en) * | 2010-09-30 | 2011-02-16 | 北京意科乐生态科技有限公司 | Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof |
| CN102524307A (en) * | 2011-12-21 | 2012-07-04 | 中国林业科学研究院森林生态环境与保护研究所 | Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof |
| CN105802863A (en) * | 2016-06-01 | 2016-07-27 | 东北农业大学 | Trichoderma harzianum fermentation process, wettable powder and application thereof |
| CN106365912A (en) * | 2016-08-31 | 2017-02-01 | 山东胜伟园林科技有限公司 | Microbial organic fertilizer capable of improving saline-alkali soil and preparation method thereof |
| CN106701608A (en) * | 2015-08-03 | 2017-05-24 | 佛山市绿葵环保科技有限公司 | Microecological modulator |
| CN107619334A (en) * | 2016-07-21 | 2018-01-23 | 济南航晨生物科技有限公司 | A kind of special ocean type disease-resistant compound microbial fertilizer of the red sage root |
| CN108017445A (en) * | 2017-12-05 | 2018-05-11 | 菏泽金正大生态工程有限公司 | A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof |
| CN108203333A (en) * | 2016-12-17 | 2018-06-26 | 营口富里实业有限公司 | A kind of microbial manure in suitable salt-soda soil |
| CN110272833A (en) * | 2019-08-05 | 2019-09-24 | 海南大学 | Trichoderma harzianum HL119 and its application |
| CN110511875A (en) * | 2019-05-29 | 2019-11-29 | 东北农业大学 | A kind of compounding biocontrol agent and the preparation method and application thereof |
| CN111205132A (en) * | 2020-02-16 | 2020-05-29 | 甘肃省农业科学院旱地农业研究所 | Potato seed dressing agent and preparation method thereof |
| CN111548975A (en) * | 2020-06-17 | 2020-08-18 | 潍坊市华滨生物科技有限公司 | Enzyme microorganism seed dressing agent and preparation and use methods thereof |
| CN111676177A (en) * | 2020-07-23 | 2020-09-18 | 中国科学院成都生物研究所 | Germination growth-promoting bacterium composition and application thereof |
| CN111908969A (en) * | 2020-08-14 | 2020-11-10 | 哈尔滨艾加生物技术有限公司 | Microbial fermentation fertilizer for preventing root rot of rice seedlings in cold regions and preparation method and use method thereof |
| CN111943760A (en) * | 2020-08-26 | 2020-11-17 | 山西农大绿荣科技有限公司 | Seed dressing agent for nitrogen-fixing bacterial manure of lichens |
| CN112457138A (en) * | 2020-12-21 | 2021-03-09 | 东北农业大学 | Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof |
| CN112501052A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof |
-
2020
- 2020-11-21 CN CN202011315433.8A patent/CN112586511B/en active Active
Patent Citations (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5985795A (en) * | 1996-10-04 | 1999-11-16 | Akio Suganuma | Antimicrobial composition |
| CN101974428A (en) * | 2010-09-30 | 2011-02-16 | 北京意科乐生态科技有限公司 | Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof |
| CN102524307A (en) * | 2011-12-21 | 2012-07-04 | 中国林业科学研究院森林生态环境与保护研究所 | Compound microorganism bacterium agent for preventing and treating meloidogyne and preparation method thereof |
| CN106701608A (en) * | 2015-08-03 | 2017-05-24 | 佛山市绿葵环保科技有限公司 | Microecological modulator |
| CN105802863A (en) * | 2016-06-01 | 2016-07-27 | 东北农业大学 | Trichoderma harzianum fermentation process, wettable powder and application thereof |
| CN107619334A (en) * | 2016-07-21 | 2018-01-23 | 济南航晨生物科技有限公司 | A kind of special ocean type disease-resistant compound microbial fertilizer of the red sage root |
| CN106365912A (en) * | 2016-08-31 | 2017-02-01 | 山东胜伟园林科技有限公司 | Microbial organic fertilizer capable of improving saline-alkali soil and preparation method thereof |
| CN108203333A (en) * | 2016-12-17 | 2018-06-26 | 营口富里实业有限公司 | A kind of microbial manure in suitable salt-soda soil |
| CN108017445A (en) * | 2017-12-05 | 2018-05-11 | 菏泽金正大生态工程有限公司 | A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof |
| CN110511875A (en) * | 2019-05-29 | 2019-11-29 | 东北农业大学 | A kind of compounding biocontrol agent and the preparation method and application thereof |
| CN110272833A (en) * | 2019-08-05 | 2019-09-24 | 海南大学 | Trichoderma harzianum HL119 and its application |
| CN111205132A (en) * | 2020-02-16 | 2020-05-29 | 甘肃省农业科学院旱地农业研究所 | Potato seed dressing agent and preparation method thereof |
| CN111548975A (en) * | 2020-06-17 | 2020-08-18 | 潍坊市华滨生物科技有限公司 | Enzyme microorganism seed dressing agent and preparation and use methods thereof |
| CN111676177A (en) * | 2020-07-23 | 2020-09-18 | 中国科学院成都生物研究所 | Germination growth-promoting bacterium composition and application thereof |
| CN111908969A (en) * | 2020-08-14 | 2020-11-10 | 哈尔滨艾加生物技术有限公司 | Microbial fermentation fertilizer for preventing root rot of rice seedlings in cold regions and preparation method and use method thereof |
| CN111943760A (en) * | 2020-08-26 | 2020-11-17 | 山西农大绿荣科技有限公司 | Seed dressing agent for nitrogen-fixing bacterial manure of lichens |
| CN112501052A (en) * | 2020-11-05 | 2021-03-16 | 东北农业大学 | Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof |
| CN112457138A (en) * | 2020-12-21 | 2021-03-09 | 东北农业大学 | Microbial preparation for improving soil fertility of saline-alkali soil and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| MANDIRIZA G ET AL: "Seed health and germination of Brassica spp. from seed companies in South Africa", 《ACTA HORTICULTURAE》 * |
| VANOVA E. P. ET AL: "A NEW NATURAL STIMULATOR 4-HYDROXYPHENETHYL ALCOHOL EFFECTS ON AMARANTH SEEDS GERMINATION AND PLANT PRODUCTIVITY", 《SEL"SKOKHOZYAISTVENNAYA BIOLOGIYA》 * |
| 李健: "高效耐盐菌的筛选鉴定及特性研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349223A (en) * | 2021-07-01 | 2021-09-07 | 黑龙江佳星农业科技开发有限公司 | Plant microorganism seed dressing agent and use method thereof |
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